Csor.00g196570 (gene) Silver-seed gourd (wild; sororia) v1

BLAST of Csor.00g196570 vs. ExPASy Swiss-Prot
Match: O24146 (4-coumarate--CoA ligase 2 OS=Nicotiana tabacum OX=4097 GN=4CL2 PE=1 SV=1)

HSP 1 Score: 798.5 bits (2061), Expect = 4.7e-230
Identity = 401/535 (74.95%), Postives = 450/535 (84.11%), Query Frame = 0

Query: 8   DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAARKAAS 67
           D IFRSKLPDIYIP HLPLHSYC  E ++E   + CLIN    + +TY+DV+L +RK A+
Sbjct: 9   DIIFRSKLPDIYIPNHLPLHSYCF-ENISEFSSRPCLINGANKQIYTYADVELNSRKVAA 68

Query: 68  GLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKAKL 127
           GL+K GI   D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+ KQAK S AK+
Sbjct: 69  GLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKI 128

Query: 128 IITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEISPDDVVAL 187
           I+TQ+ +  KVK+   +  D KI+ +DS P GCL F+ L Q  +H+IP VEI PDDVVAL
Sbjct: 129 IVTQACHVNKVKDYAFE-NDVKIICIDSAPEGCLHFSVLTQANEHDIPEVEIQPDDVVAL 188

Query: 188 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIYSLNSVLL 247
           PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY  ++DV+LCVLPLFHIYSLNSVLL
Sbjct: 189 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNSVLL 248

Query: 248 CGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSSIRMIK 307
           CGLR GA ILIM KF+I S L+L+++Y+V+I P VPPIVLAIAKSP ++ YDLSS+R + 
Sbjct: 249 CGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVRTVM 308

Query: 308 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 367
           SG APLGKELEDTVRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACGTVVRN
Sbjct: 309 SGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTVVRN 368

Query: 368 AEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTGDIGLIDD 427
           AE+KIVD + G SLPRN  GEICIRGDQIMKGYLN+PEATA TID EGWL+TGDIG IDD
Sbjct: 369 AEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGYIDD 428

Query: 428 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKL 487
           DDELFIVDRLKELIKYKGFQVAPAELEALLL HP ISDAAVVPMKDEQAGEVPVAFVV+ 
Sbjct: 429 DDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRS 488

Query: 488 KNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAADFP 543
             S +TEDE+K FISKQV+FYKRI RVFF+DAIPKSPSGKILRK+LRAKLAA  P
Sbjct: 489 NGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLP 541

BLAST of Csor.00g196570 vs. ExPASy Swiss-Prot
Match: O24145 (4-coumarate--CoA ligase 1 OS=Nicotiana tabacum OX=4097 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 8.9e-229
Identity = 394/540 (72.96%), Postives = 448/540 (82.96%), Query Frame = 0

Query: 3   TETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAA 62
           T+   D IFRSKLPDIYIPKHLPLHSYC  E ++E   + CLIN    + +TY++V+L  
Sbjct: 9   TKQSGDLIFRSKLPDIYIPKHLPLHSYCF-ENISEFSSRPCLINGANDQIYTYAEVELTC 68

Query: 63  RKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKG 122
           RK A GL KLGI + D IM+LLPNSPEFVFAF+GASYLGAI T ANP FT AE+ KQAK 
Sbjct: 69  RKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKA 128

Query: 123 SKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEISPD 182
           S AK+IITQS +  KVK+   +  D K++ +DS P GCL F++L Q  +HEIP V+I PD
Sbjct: 129 SSAKIIITQSCFVGKVKDYASE-NDVKVICIDSAPEGCLHFSELTQSDEHEIPEVKIQPD 188

Query: 183 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIYSL 242
           DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGEN NLY  ++DV++CVLPLFHIYSL
Sbjct: 189 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSL 248

Query: 243 NSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSS 302
           NS+LLCGLR GA ILIM KF+I   L+L++KY+VSI P VPPIVLAIAKSP ++ YDLSS
Sbjct: 249 NSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDSYDLSS 308

Query: 303 IRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 362
           +R + SG APLGKELED VR KFP A LGQGYGMTEAGPVL M LAFAKEPF +K GACG
Sbjct: 309 VRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACG 368

Query: 363 TVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTGDI 422
           TVVRNAE+KIVD + G SLPRN PGEICIRGDQIMKGYLN+PEAT  TID EGWLHTGDI
Sbjct: 369 TVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWLHTGDI 428

Query: 423 GLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 482
           G ID+DDELFIVDRLKELIKYKGFQVAPAE+EALLL HP ISDAAVVPMKDEQAGEVPVA
Sbjct: 429 GFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAGEVPVA 488

Query: 483 FVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAADFP 542
           FVV+   S +TEDE+K FISKQV+FYKR+ RVFF++ +PKSPSGKILRK+LRA+LAA  P
Sbjct: 489 FVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARLAAGVP 546

BLAST of Csor.00g196570 vs. ExPASy Swiss-Prot
Match: O24540 (4-coumarate--CoA ligase OS=Vanilla planifolia OX=51239 GN=4CL PE=3 SV=1)

HSP 1 Score: 785.8 bits (2028), Expect = 3.2e-226
Identity = 401/541 (74.12%), Postives = 451/541 (83.36%), Query Frame = 0

Query: 4   ETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAAR 63
           E   D IFRSKLPDIYIPK+LPLHSYC  E +++   + CLIN  T E FTY+DV+L +R
Sbjct: 9   EQKKDIIFRSKLPDIYIPKNLPLHSYCF-ENISKFSSRPCLINGATDEIFTYADVELISR 68

Query: 64  KAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGS 123
           +  SGL KLGI +GD IM+LLPNSPEFVFAFLGAS++G+I T ANPFFT+ E+ KQAK S
Sbjct: 69  RVGSGLSKLGIKQGDTIMILLPNSPEFVFAFLGASFIGSISTMANPFFTSTEVIKQAKAS 128

Query: 124 KAKLIITQSSYCEKVKEITEQLPDAKIMTVDS-----PPLGCLSFADLIQGGDHEIPAVE 183
            AKLIITQ  Y +KVK+   +    KI+++D+          L F++L    ++E+P VE
Sbjct: 129 NAKLIITQGCYVDKVKDYACE-NGVKIISIDTTTTADDAANILHFSELTGADENEMPKVE 188

Query: 184 ISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFH 243
           ISPD VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY  +DDV+LCVLPLFH
Sbjct: 189 ISPDGVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYMHSDDVLLCVLPLFH 248

Query: 244 IYSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKY 303
           IYSLNSVLLCGLRAG+ ILIM KFEI   L+L++KY+V+I P VPPIVLAIAKS  ++ Y
Sbjct: 249 IYSLNSVLLCGLRAGSGILIMQKFEIVPFLELIQKYKVTIGPFVPPIVLAIAKSTVVDNY 308

Query: 304 DLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKP 363
           DLSS+R + SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEPF +K 
Sbjct: 309 DLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKS 368

Query: 364 GACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLH 423
           GACGTVVRNAE+KIVD E G SLPRN PGEICIRGDQIMKGYLN+PEATA TID EGWLH
Sbjct: 369 GACGTVVRNAEMKIVDPETGSSLPRNHPGEICIRGDQIMKGYLNDPEATARTIDKEGWLH 428

Query: 424 TGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGE 483
           TGDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE AGE
Sbjct: 429 TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPCISDAAVVPMKDEAAGE 488

Query: 484 VPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 540
           VPVAFVVK     +TEDEIKQFISKQV+FYKRINRVFF++AIPK+PSGKILRK+LRA+LA
Sbjct: 489 VPVAFVVKSNGHNITEDEIKQFISKQVIFYKRINRVFFVEAIPKAPSGKILRKDLRARLA 547

BLAST of Csor.00g196570 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 9.2e-226
Identity = 392/536 (73.13%), Postives = 453/536 (84.51%), Query Frame = 0

Query: 8   DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAARKAAS 67
           D IFRSKLPDIYIPKHLPLH+YC  E ++++G ++CLIN  TGE+FTYS V+L +RK AS
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 69

Query: 68  GLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKAKL 127
           GL KLGI +GD IMLLLPNSPE+ FAFLGASY GAI T ANPFFT+AE+ KQ K S+AKL
Sbjct: 70  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 129

Query: 128 IITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEISPDDVVAL 187
           IITQ+ Y +KVK+   +  + +I+ +D  P  CL F+ L++  + E+P V I+ DDVVAL
Sbjct: 130 IITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVINSDDVVAL 189

Query: 188 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIYSLNSVLL 247
           PYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY  ++DV++C+LPLFHIYSLN+VL 
Sbjct: 190 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLC 249

Query: 248 CGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSSIRMIK 307
           CGLRAG TILIM KF+I   L+L++KY+V+I P VPPIVLAIAKSP ++KYDLSS+R + 
Sbjct: 250 CGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVM 309

Query: 308 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 367
           SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVVRN
Sbjct: 310 SGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRN 369

Query: 368 AEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTGDIGLIDD 427
           AE+KIVD E   SLPRN  GEICIRGDQIMKGYLN+PE+T  TID EGWLHTGDIG IDD
Sbjct: 370 AEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDD 429

Query: 428 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKL 487
           DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV+ 
Sbjct: 430 DDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRT 489

Query: 488 KNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA-DFP 543
                TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRA++A+ D P
Sbjct: 490 NGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARIASGDLP 543

BLAST of Csor.00g196570 vs. ExPASy Swiss-Prot
Match: P14913 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL2 PE=2 SV=1)

HSP 1 Score: 783.9 bits (2023), Expect = 1.2e-225
Identity = 393/536 (73.32%), Postives = 451/536 (84.14%), Query Frame = 0

Query: 8   DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAARKAAS 67
           D IFRSKLPDIYIPKHLPLH+YC  E ++++G ++CLIN  TGE+FTYS V+L +RK AS
Sbjct: 10  DLIFRSKLPDIYIPKHLPLHTYCF-ENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 69

Query: 68  GLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKAKL 127
           GL KLGI +GD IMLLLPNSPE+ FAFLGASY GAI T ANPFFT+AE+ KQ K S AKL
Sbjct: 70  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASLAKL 129

Query: 128 IITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEISPDDVVAL 187
           IITQ+ Y +KVK+   +  + +I+ +D  P  CL F+ L++  + E+P V I  DDVVAL
Sbjct: 130 IITQACYVDKVKDYAAE-KNIQIICIDDAPQDCLHFSKLMEADESEMPEVVIDSDDVVAL 189

Query: 188 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIYSLNSVLL 247
           PYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY  ++DV++C+LPLFHIYSLN+VL 
Sbjct: 190 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAVLC 249

Query: 248 CGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSSIRMIK 307
           CGLRAG TILIM KF+I   L+L++KY+V+I P VPPIVLAIAKSP ++KYDLSS+R + 
Sbjct: 250 CGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVRTVM 309

Query: 308 SGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVVRN 367
           SG APLGKELED VRAKFP A LGQGYGMTEAGPVL M LAFAKEP+ +K GACGTVVRN
Sbjct: 310 SGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGTVVRN 369

Query: 368 AEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTGDIGLIDD 427
           AE+KIVD E   SLPRN  GEICIRGDQIMKGYLN+PE+T  TID EGWLHTGDIG IDD
Sbjct: 370 AEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIGFIDD 429

Query: 428 DDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVVKL 487
           DDELFIVDRLKE+IKYKGFQVAPAELEALLLTHP ISDAAVVPM DE+AGEVPVAFVV+ 
Sbjct: 430 DDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAFVVRT 489

Query: 488 KNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA-DFP 543
                TE+EIKQF+SKQVVFYKRI RVFF+DAIPKSPSGKILRK+LRAK+A+ D P
Sbjct: 490 NGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRAKIASGDLP 543

BLAST of Csor.00g196570 vs. NCBI nr
Match: KAG6606850.1 (4-coumarate--CoA ligase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1075 bits (2780), Expect = 0.0
Identity = 545/545 (100.00%), Postives = 545/545 (100.00%), Query Frame = 0

Query: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60
           MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL
Sbjct: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60

Query: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120
           AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA
Sbjct: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120

Query: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180
           KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS
Sbjct: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180

Query: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240

Query: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300
           SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL
Sbjct: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300

Query: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360
           SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA
Sbjct: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360

Query: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTG 420
           CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTG
Sbjct: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTG 420

Query: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480
           DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540
           VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD
Sbjct: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540

Query: 541 FPIPN 545
           FPIPN
Sbjct: 541 FPIPN 545

BLAST of Csor.00g196570 vs. NCBI nr
Match: XP_023524772.1 (4-coumarate--CoA ligase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1066 bits (2757), Expect = 0.0
Identity = 541/545 (99.27%), Postives = 544/545 (99.82%), Query Frame = 0

Query: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60
           MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGH+TCLINAVTGESFTYSDVDL
Sbjct: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVDL 60

Query: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120
           AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA
Sbjct: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120

Query: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180
           KGSKAKLIITQSSY EKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS
Sbjct: 121 KGSKAKLIITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180

Query: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240

Query: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300
           SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL
Sbjct: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300

Query: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360
           SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA
Sbjct: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360

Query: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTG 420
           CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+EGWLHTG
Sbjct: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHTG 420

Query: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480
           DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDE+AGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEEAGEVP 480

Query: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540
           VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD
Sbjct: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540

Query: 541 FPIPN 545
           FPIPN
Sbjct: 541 FPIPN 545

BLAST of Csor.00g196570 vs. NCBI nr
Match: XP_022998425.1 (4-coumarate--CoA ligase 1-like [Cucurbita maxima])

HSP 1 Score: 1058 bits (2735), Expect = 0.0
Identity = 537/545 (98.53%), Postives = 540/545 (99.08%), Query Frame = 0

Query: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60
           MA ETVHD IFRSKLPDIYIPKHLPLHSYCLQEKMAEIGH+TCLINAVTGESFTYSDVDL
Sbjct: 1   MANETVHDIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVDL 60

Query: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120
           AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA
Sbjct: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120

Query: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180
           KGSKAKL ITQSSY EKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS
Sbjct: 121 KGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180

Query: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240

Query: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300
           SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL
Sbjct: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300

Query: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360
           SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA
Sbjct: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360

Query: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTG 420
           CGTVVRNAEIKIVDTENGGSLPRNTPGEICI+GDQIMKGYLNNPEATAATIDNEGWLHTG
Sbjct: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHTG 420

Query: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480
           DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540
           VAFVVKLKNS+VTEDEIKQFISKQVVFYKRINRVFFI AIPKSPSGKILRKELRAKLAAD
Sbjct: 481 VAFVVKLKNSKVTEDEIKQFISKQVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLAAD 540

Query: 541 FPIPN 545
           FPIPN
Sbjct: 541 FPIPN 545

BLAST of Csor.00g196570 vs. NCBI nr
Match: XP_022948656.1 (4-coumarate--CoA ligase 1-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 538/544 (98.90%), Postives = 541/544 (99.45%), Query Frame = 0

Query: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60
           MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGH+TCLINAVTGESFTYSDVDL
Sbjct: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVDL 60

Query: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120
           AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA
Sbjct: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120

Query: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180
           KGSKAKLIITQSSY EKVKEITEQL DAKIMTVDSP LGCLSFADLIQGGDH+IPAVEIS
Sbjct: 121 KGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEIS 180

Query: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240

Query: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300
           SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL
Sbjct: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300

Query: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360
           SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA
Sbjct: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360

Query: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTG 420
           CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+EGWLHTG
Sbjct: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHTG 420

Query: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480
           DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540
           VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD
Sbjct: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540

Query: 541 FPIP 544
           FPIP
Sbjct: 541 FPIP 544

BLAST of Csor.00g196570 vs. NCBI nr
Match: XP_022948655.1 (4-coumarate--CoA ligase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1047 bits (2708), Expect = 0.0
Identity = 538/559 (96.24%), Postives = 541/559 (96.78%), Query Frame = 0

Query: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60
           MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGH+TCLINAVTGESFTYSDVDL
Sbjct: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVDL 60

Query: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120
           AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA
Sbjct: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120

Query: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIP----- 180
           KGSKAKLIITQSSY EKVKEITEQL DAKIMTVDSP LGCLSFADLIQGGDH+IP     
Sbjct: 121 KGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPVSFAD 180

Query: 181 ----------AVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYR 240
                     AVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYR
Sbjct: 181 LIQGGDHKIPAVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYR 240

Query: 241 NDDVILCVLPLFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPI 300
           NDDVILCVLPLFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPI
Sbjct: 241 NDDVILCVLPLFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPI 300

Query: 301 VLAIAKSPDLEKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTM 360
           VLAIAKSPDLEKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTM
Sbjct: 301 VLAIAKSPDLEKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTM 360

Query: 361 GLAFAKEPFPMKPGACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPE 420
           GLAFAKEPFPMKPGACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPE
Sbjct: 361 GLAFAKEPFPMKPGACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPE 420

Query: 421 ATAATIDNEGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISD 480
           ATAATID+EGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISD
Sbjct: 421 ATAATIDSEGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISD 480

Query: 481 AAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPS 540
           AAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPS
Sbjct: 481 AAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPS 540

Query: 541 GKILRKELRAKLAADFPIP 544
           GKILRKELRAKLAADFPIP
Sbjct: 541 GKILRKELRAKLAADFPIP 559

BLAST of Csor.00g196570 vs. ExPASy TrEMBL
Match: A0A6J1KCG9 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111493060 PE=4 SV=1)

HSP 1 Score: 1058 bits (2735), Expect = 0.0
Identity = 537/545 (98.53%), Postives = 540/545 (99.08%), Query Frame = 0

Query: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60
           MA ETVHD IFRSKLPDIYIPKHLPLHSYCLQEKMAEIGH+TCLINAVTGESFTYSDVDL
Sbjct: 1   MANETVHDIIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVDL 60

Query: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120
           AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA
Sbjct: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120

Query: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180
           KGSKAKL ITQSSY EKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS
Sbjct: 121 KGSKAKLTITQSSYYEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180

Query: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240

Query: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300
           SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL
Sbjct: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300

Query: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360
           SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA
Sbjct: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360

Query: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTG 420
           CGTVVRNAEIKIVDTENGGSLPRNTPGEICI+GDQIMKGYLNNPEATAATIDNEGWLHTG
Sbjct: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIKGDQIMKGYLNNPEATAATIDNEGWLHTG 420

Query: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480
           DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540
           VAFVVKLKNS+VTEDEIKQFISKQVVFYKRINRVFFI AIPKSPSGKILRKELRAKLAAD
Sbjct: 481 VAFVVKLKNSKVTEDEIKQFISKQVVFYKRINRVFFIGAIPKSPSGKILRKELRAKLAAD 540

Query: 541 FPIPN 545
           FPIPN
Sbjct: 541 FPIPN 545

BLAST of Csor.00g196570 vs. ExPASy TrEMBL
Match: A0A6J1G9T7 (4-coumarate--CoA ligase 1-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)

HSP 1 Score: 1057 bits (2734), Expect = 0.0
Identity = 538/544 (98.90%), Postives = 541/544 (99.45%), Query Frame = 0

Query: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60
           MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGH+TCLINAVTGESFTYSDVDL
Sbjct: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVDL 60

Query: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120
           AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA
Sbjct: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120

Query: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEIS 180
           KGSKAKLIITQSSY EKVKEITEQL DAKIMTVDSP LGCLSFADLIQGGDH+IPAVEIS
Sbjct: 121 KGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPAVEIS 180

Query: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240
           PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY
Sbjct: 181 PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIY 240

Query: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300
           SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL
Sbjct: 241 SLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDL 300

Query: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360
           SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA
Sbjct: 301 SSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGA 360

Query: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTG 420
           CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID+EGWLHTG
Sbjct: 361 CGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDSEGWLHTG 420

Query: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480
           DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP
Sbjct: 421 DIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVP 480

Query: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540
           VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD
Sbjct: 481 VAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAAD 540

Query: 541 FPIP 544
           FPIP
Sbjct: 541 FPIP 544

BLAST of Csor.00g196570 vs. ExPASy TrEMBL
Match: A0A6J1GAH7 (4-coumarate--CoA ligase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111452267 PE=4 SV=1)

HSP 1 Score: 1047 bits (2708), Expect = 0.0
Identity = 538/559 (96.24%), Postives = 541/559 (96.78%), Query Frame = 0

Query: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDL 60
           MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGH+TCLINAVTGESFTYSDVDL
Sbjct: 1   MATETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHRTCLINAVTGESFTYSDVDL 60

Query: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120
           AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA
Sbjct: 61  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 120

Query: 121 KGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIP----- 180
           KGSKAKLIITQSSY EKVKEITEQL DAKIMTVDSP LGCLSFADLIQGGDH+IP     
Sbjct: 121 KGSKAKLIITQSSYYEKVKEITEQLSDAKIMTVDSPSLGCLSFADLIQGGDHKIPVSFAD 180

Query: 181 ----------AVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYR 240
                     AVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYR
Sbjct: 181 LIQGGDHKIPAVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYR 240

Query: 241 NDDVILCVLPLFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPI 300
           NDDVILCVLPLFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPI
Sbjct: 241 NDDVILCVLPLFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPI 300

Query: 301 VLAIAKSPDLEKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTM 360
           VLAIAKSPDLEKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTM
Sbjct: 301 VLAIAKSPDLEKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTM 360

Query: 361 GLAFAKEPFPMKPGACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPE 420
           GLAFAKEPFPMKPGACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPE
Sbjct: 361 GLAFAKEPFPMKPGACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPE 420

Query: 421 ATAATIDNEGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISD 480
           ATAATID+EGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISD
Sbjct: 421 ATAATIDSEGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISD 480

Query: 481 AAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPS 540
           AAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPS
Sbjct: 481 AAVVPMKDEQAGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPS 540

Query: 541 GKILRKELRAKLAADFPIP 544
           GKILRKELRAKLAADFPIP
Sbjct: 541 GKILRKELRAKLAADFPIP 559

BLAST of Csor.00g196570 vs. ExPASy TrEMBL
Match: A0A6J1F599 (4-coumarate--CoA ligase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111442288 PE=4 SV=1)

HSP 1 Score: 972 bits (2514), Expect = 0.0
Identity = 491/546 (89.93%), Postives = 516/546 (94.51%), Query Frame = 0

Query: 1   MATETV-HDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVD 60
           MA E + +DFIFRSKLPDIYIP HLPLHSYCL E +A+IGH+TCLIN+VTGESFTY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINSVTGESFTYHDVD 60

Query: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           LAARK ASGL KLGIA+GDVIML+L NSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVIMLILQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180
           AKGS AKLI+TQSSY EKVKEITE+LPD KIMTVDSPP GCLSFADLIQ  + E+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLYYRN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLLQLV+K++VSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHT 420
           ACGTVVRNAE+KIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAE+EALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAEVEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLAA
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAA 540

Query: 541 DFPIPN 545
            FP  N
Sbjct: 541 GFPNSN 546

BLAST of Csor.00g196570 vs. ExPASy TrEMBL
Match: A0A6J1J912 (4-coumarate--CoA ligase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482411 PE=4 SV=1)

HSP 1 Score: 968 bits (2503), Expect = 0.0
Identity = 490/546 (89.74%), Postives = 514/546 (94.14%), Query Frame = 0

Query: 1   MATETV-HDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVD 60
           MA E + +DFIFRSKLPDIYIP HLPLHSYCL E +A+IGH+TCLINAVT ESFTY DVD
Sbjct: 1   MAVEALQNDFIFRSKLPDIYIPNHLPLHSYCLHENLAKIGHRTCLINAVTAESFTYHDVD 60

Query: 61  LAARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQ 120
           LAARK ASGL KLGIA+GDVI+LLL NSPEFVFAFLGASY GAIMTAANPFFTAAEIAKQ
Sbjct: 61  LAARKVASGLNKLGIAQGDVILLLLQNSPEFVFAFLGASYRGAIMTAANPFFTAAEIAKQ 120

Query: 121 AKGSKAKLIITQSSYCEKVKEITEQLPDAKIMTVDSPPLGCLSFADLIQGGDHEIPAVEI 180
           AKGS AKLI+TQSSY EKVKEITE+LPD KIMTVDSPP GCLSFADLIQ  + E+P VEI
Sbjct: 121 AKGSNAKLIVTQSSYYEKVKEITEELPDVKIMTVDSPPDGCLSFADLIQADEREMPTVEI 180

Query: 181 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 240
           +PDDVVALPYSSGTTGLPKGVMLTH+GLVTSVAQQVDGENPNLYYRN+DVILCVLPLFHI
Sbjct: 181 NPDDVVALPYSSGTTGLPKGVMLTHRGLVTSVAQQVDGENPNLYYRNEDVILCVLPLFHI 240

Query: 241 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 300
           YSLNSVLLCGLRAG TILIMPKFEIGSLLQLV+K++VSIAPIVPPIVLAIAKSPDL+KYD
Sbjct: 241 YSLNSVLLCGLRAGTTILIMPKFEIGSLLQLVQKFKVSIAPIVPPIVLAIAKSPDLDKYD 300

Query: 301 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360
           LSSIR+IK GGAPLGKELED+VRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG
Sbjct: 301 LSSIRVIKCGGAPLGKELEDSVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 360

Query: 361 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHT 420
           ACGTVVRNAE+KIVDTENG SLPRNTPGEICIRGDQIMKGYLNNPEATAATID +GWLHT
Sbjct: 361 ACGTVVRNAEMKIVDTENGCSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDKDGWLHT 420

Query: 421 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 480
           GDIG IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHP ISDAAVVPMKDE+AGEV
Sbjct: 421 GDIGFIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPAISDAAVVPMKDEEAGEV 480

Query: 481 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLAA 540
           PVAFVV+LKNSEVTEDEIKQFISKQVVFYKRI R F IDAIPKSPSGKILRKELRAKLA 
Sbjct: 481 PVAFVVRLKNSEVTEDEIKQFISKQVVFYKRIKRAFMIDAIPKSPSGKILRKELRAKLAT 540

Query: 541 DFPIPN 545
            FPI N
Sbjct: 541 GFPISN 546

BLAST of Csor.00g196570 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 756.9 bits (1953), Expect = 1.1e-218
Identity = 376/536 (70.15%), Postives = 450/536 (83.96%), Query Frame = 0

Query: 7   HDFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAARKAA 66
           +D IFRS+LPDIYIP HLPLH Y + E ++E   + CLIN  TGE +TY+DV + +RK A
Sbjct: 19  NDVIFRSRLPDIYIPNHLPLHDY-IFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLA 78

Query: 67  SGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKAK 126
           +GL+ LG+ + DV+M+LLPNSPE V  FL AS++GAI T+ANPFFT AEI+KQAK S AK
Sbjct: 79  AGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAK 138

Query: 127 LIITQSSYCEKVKEITEQLPDAKIMTVDSP--PLGCLSFADLIQGGDHEIPAV--EISPD 186
           LI+TQS Y +K+K +  Q     I+T DS   P  CL F++L Q  +  + ++  +ISP+
Sbjct: 139 LIVTQSRYVDKIKNL--QNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDSIPEKISPE 198

Query: 187 DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIYSL 246
           DVVALP+SSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+  DDVILCVLP+FHIY+L
Sbjct: 199 DVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYAL 258

Query: 247 NSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSS 306
           NS++LC LR GATILIMPKFEI  LL+ +++ +V++A +VPPIVLAIAKSP+ EKYDLSS
Sbjct: 259 NSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSS 318

Query: 307 IRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACG 366
           +RM+KSG APLGKELED + AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K GACG
Sbjct: 319 VRMVKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACG 378

Query: 367 TVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTGDI 426
           TVVRNAE+KI+D + G SLPRN PGEICIRG+QIMKGYLN+P ATA+TID +GWLHTGD+
Sbjct: 379 TVVRNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDV 438

Query: 427 GLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVA 486
           G IDDDDELFIVDRLKELIKYKGFQVAPAELE+LL+ HP I+D AVV MK+E AGEVPVA
Sbjct: 439 GFIDDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVA 498

Query: 487 FVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 539
           FVV+ K+S ++EDEIKQF+SKQVVFYKRIN+VFF D+IPK+PSGKILRK+LRA+LA
Sbjct: 499 FVVRSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARLA 551

BLAST of Csor.00g196570 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 754.6 bits (1947), Expect = 5.5e-218
Identity = 383/539 (71.06%), Postives = 441/539 (81.82%), Query Frame = 0

Query: 8   DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAARKAAS 67
           D IFRSKLPDIYIP HL LH Y  Q  ++E   + CLIN  TG  +TYSDV + +R+ A+
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIAA 81

Query: 68  GLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKAKL 127
             +KLG+ + DV+MLLLPN PEFV +FL AS+ GA  TAANPFFT AEIAKQAK S  KL
Sbjct: 82  NFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKL 141

Query: 128 IITQSSYCEKVKEITEQLPDAKIMTVDSP-----PLGCLSFADLIQG---GDHEIPAVEI 187
           IIT++ Y +K+K +        I+ +D       P GCL F +L Q        I +VEI
Sbjct: 142 IITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 201

Query: 188 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 247
           SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+ +DDVILCVLP+FHI
Sbjct: 202 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 261

Query: 248 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 307
           Y+LNS++LCGLR GA ILIMPKFEI  LL+L+++ +V++AP+VPPIVLAIAKS + EKYD
Sbjct: 262 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 321

Query: 308 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 367
           LSSIR++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K G
Sbjct: 322 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 381

Query: 368 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHT 427
           ACGTVVRNAE+KIVD + G SL RN PGEICIRG QIMKGYLNNP ATA TID +GWLHT
Sbjct: 382 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 441

Query: 428 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 487
           GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 442 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 501

Query: 488 PVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKLA 539
           PVAFVVK K+SE++ED++KQF+SKQVVFYKRIN+VFF ++IPK+PSGKILRK+LRAKLA
Sbjct: 502 PVAFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKLA 558

BLAST of Csor.00g196570 vs. TAIR 10
Match: AT1G51680.3 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 699.5 bits (1804), Expect = 2.1e-201
Identity = 356/506 (70.36%), Postives = 409/506 (80.83%), Query Frame = 0

Query: 8   DFIFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAARKAAS 67
           D IFRSKLPDIYIP HL LH Y  Q  ++E   + CLIN  TG  +TYSDV + +R+ A+
Sbjct: 22  DVIFRSKLPDIYIPNHLSLHDYIFQ-NISEFATKPCLINGPTGHVYTYSDVHVISRQIAA 81

Query: 68  GLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKAKL 127
             +KLG+ + DV+MLLLPN PEFV +FL AS+ GA  TAANPFFT AEIAKQAK S  KL
Sbjct: 82  NFHKLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKL 141

Query: 128 IITQSSYCEKVKEITEQLPDAKIMTVDSP-----PLGCLSFADLIQG---GDHEIPAVEI 187
           IIT++ Y +K+K +        I+ +D       P GCL F +L Q        I +VEI
Sbjct: 142 IITEARYVDKIKPLQND-DGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEI 201

Query: 188 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHI 247
           SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLY+ +DDVILCVLP+FHI
Sbjct: 202 SPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHI 261

Query: 248 YSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYD 307
           Y+LNS++LCGLR GA ILIMPKFEI  LL+L+++ +V++AP+VPPIVLAIAKS + EKYD
Sbjct: 262 YALNSIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYD 321

Query: 308 LSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPG 367
           LSSIR++KSG APLGKELED V AKFP A LGQGYGMTEAGPVL M L FAKEPFP+K G
Sbjct: 322 LSSIRVVKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSG 381

Query: 368 ACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHT 427
           ACGTVVRNAE+KIVD + G SL RN PGEICIRG QIMKGYLNNP ATA TID +GWLHT
Sbjct: 382 ACGTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHT 441

Query: 428 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEV 487
           GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ HP I+D AVV MK+E AGEV
Sbjct: 442 GDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEV 501

Query: 488 PVAFVVKLKNSEVTEDEIKQFISKQV 506
           PVAFVVK K+SE++ED++KQF+SKQV
Sbjct: 502 PVAFVVKSKDSELSEDDVKQFVSKQV 525

BLAST of Csor.00g196570 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 692.2 bits (1785), Expect = 3.4e-199
Identity = 344/532 (64.66%), Postives = 430/532 (80.83%), Query Frame = 0

Query: 10  IFRSKLPDIYIPKHLPLHSYCLQEKMAEIGHQTCLINAVTGESFTYSDVDLAARKAASGL 69
           IFRSKLPDI IP HLPLH+YC  EK++ +  + CLI   TG+S+TY +  L  R+ ASGL
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCF-EKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGL 93

Query: 70  YKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQAKGSKAKLII 129
           YKLGI KGDVIM+LL NS EFVF+F+GAS +GA+ T ANPF+T+ E+ KQ K S AKLII
Sbjct: 94  YKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLII 153

Query: 130 TQSSYCEKVKEITEQLPDAKIMTVDSP-PLGCLSFADLIQGGDHEIP---AVEISPDDVV 189
           T S Y +K+K + E L    ++T D P P  CL F+ LI   D   P    V+I  DD  
Sbjct: 154 THSQYVDKLKNLGENL---TLITTDEPTPENCLPFSTLIT-DDETNPFQETVDIGGDDAA 213

Query: 190 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCVLPLFHIYSLNSV 249
           ALP+SSGTTGLPKGV+LTHK L+TSVAQQVDG+NPNLY +++DVILCVLPLFHIYSLNSV
Sbjct: 214 ALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSV 273

Query: 250 LLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSPDLEKYDLSSIRM 309
           LL  LR+GAT+L+M KFEIG+LL L++++RV+IA +VPP+V+A+AK+P +  YDLSS+R 
Sbjct: 274 LLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF 333

Query: 310 IKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEPFPMKPGACGTVV 369
           + SG APLGKEL+D++R + P+A+LGQGYGMTEAGPVL+M L FAKEP P K G+CGTVV
Sbjct: 334 VLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVV 393

Query: 370 RNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDNEGWLHTGDIGLI 429
           RNAE+K+V  E   SL  N PGEICIRG QIMK YLN+PEAT+ATID EGWLHTGDIG +
Sbjct: 394 RNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYLNDPEATSATIDEEGWLHTGDIGYV 453

Query: 430 DDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKDEQAGEVPVAFVV 489
           D+DDE+FIVDRLKE+IK+KGFQV PAELE+LL+ H  I+DAAVVP  DE AGEVPVAFVV
Sbjct: 454 DEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVV 513

Query: 490 KLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKELRAKL 538
           +   +++TE+++K++++KQVVFYKR+++VFF+ +IPKSPSGKILRK+L+AKL
Sbjct: 514 RSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

BLAST of Csor.00g196570 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 665.6 bits (1716), Expect = 3.4e-191
Identity = 328/544 (60.29%), Postives = 414/544 (76.10%), Query Frame = 0

Query: 4   ETVHDFIFRSKLPDIYIPKHLPLHSYCLQEKMAE---IGHQTCLINAVTGESFTYSDVDL 63
           E  HDFIFRSKLPDI+IP HLPL  Y  Q    +       TC+I+  TG   TY+DV  
Sbjct: 22  EPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYADVQT 81

Query: 64  AARKAASGLYKLGIAKGDVIMLLLPNSPEFVFAFLGASYLGAIMTAANPFFTAAEIAKQA 123
             R+ A+G+++LGI  GDV+MLLLPNSPEF  +FL  +YLGA+ T ANPF+T  EIAKQA
Sbjct: 82  NMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEIAKQA 141

Query: 124 KGSKAKLIITQSSYCEKVKEITEQ-------LPDAKIMTVDSPPLGCLSFADLIQGGDHE 183
           K S AK+IIT+    +K+  +            D     V S   GC+SF +L Q  + E
Sbjct: 142 KASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQADETE 201

Query: 184 IPAVEISPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYYRNDDVILCV 243
           +   +ISP+D VA+PYSSGTTGLPKGVM+THKGLVTS+AQ+VDGENPNL +  +DVILC 
Sbjct: 202 LLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTANDVILCF 261

Query: 244 LPLFHIYSLNSVLLCGLRAGATILIMPKFEIGSLLQLVEKYRVSIAPIVPPIVLAIAKSP 303
           LP+FHIY+L++++L  +R GA +LI+P+FE+  +++L+++Y+V++ P+ PP+VLA  KSP
Sbjct: 262 LPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVVLAFIKSP 321

Query: 304 DLEKYDLSSIRMIKSGGAPLGKELEDTVRAKFPKAVLGQGYGMTEAGPVLTMGLAFAKEP 363
           + E+YDLSS+R++ SG A L KELED VR KFP A+ GQGYGMTE+G V    LAFAK P
Sbjct: 322 ETERYDLSSVRIMLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTV-AKSLAFAKNP 381

Query: 364 FPMKPGACGTVVRNAEIKIVDTENGGSLPRNTPGEICIRGDQIMKGYLNNPEATAATIDN 423
           F  K GACGTV+RNAE+K+VDTE G SLPRN  GEIC+RG Q+MKGYLN+PEATA TID 
Sbjct: 382 FKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDPEATARTIDK 441

Query: 424 EGWLHTGDIGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLTHPVISDAAVVPMKD 483
           +GWLHTGDIG +DDDDE+FIVDRLKELIK+KG+QVAPAELEALL++HP I DAAVV MKD
Sbjct: 442 DGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKD 501

Query: 484 EQAGEVPVAFVVKLKNSEVTEDEIKQFISKQVVFYKRINRVFFIDAIPKSPSGKILRKEL 538
           E A EVPVAFV + + S++TED++K +++KQVV YKRI  VFFI+ IPK+ SGKILRK+L
Sbjct: 502 EVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDL 561