Csor.00g194700 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g194700
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	Cytochrome P450
Location	Csor_Chr01: 1934949 .. 1935726 (-)
RNA-Seq Expression	Csor.00g194700
Synteny	Csor.00g194700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATATGATACTCTCTCTTTCTACTGGCTTACCCCTAGAAGAATCAGGAAGAGGATGGCCGAGCAAGGCGTTTATGGCCCCGACCCACGACCCTTCACTGGAAACATCCTCGATGTCGTAGAGCTTGTGAAGAAATCCACGGCTAACGACATGAGCTCCATCAGTCATGACATCGTTGATCGTTTGTTACCCTATTACTCTGCATGGACAAAGCAATACGGTACTACTAACGCTTTTATCCTGTTTAAAAGAGTTCATGATTCATTGGTTAGAAAAATATCTAGTATGATTGAGGATATTAAGCCTTTCATTACCTATAGGTACCATATTTGGATCTTTGTTAGACAAATATCTATTATCAATCTTTGCGTGACATTTACTTATTGTTAATTTTATTTTGTAATTTATTTGATTTTGTAAATAATTATAAACAGAGAACTTCATTCACTCTAATTAAGATGTGGTGGTTTTAGTAAATATTTTTGTAAACTACGAATAAACAATTAACCCGTAAACTTAAACTTATTCAGGAAAGAGGTTTATATATTGGAACGGGATGGAGCCGAGGCTGTGTTTGACGGAGATGGAGTTGGTGAAGGAAGTATTGGGGAAGGGCAGCAATGTGTGTGGGAGGTCATGGATGCAAAGACAAGGGACCAAACACTTCATAGGGAAGGGATTGTTGATGGCAAATGGAGAAGCTTGGCATCATCAACGTCACCTGATTTCACCTGCGTTTAGAGGGGAAAGGCTCAAGGTTCATATTCAAACCTGA

mRNA sequence

ATGGCATATGATACTCTCTCTTTCTACTGGCTTACCCCTAGAAGAATCAGGAAGAGGATGGCCGAGCAAGGCGTTTATGGCCCCGACCCACGACCCTTCACTGGAAACATCCTCGATGTCGTAGAGCTTGTGAAGAAATCCACGGCTAACGACATGAGCTCCATCAGTCATGACATCGTTGATCGTTTGTTACCCTATTACTCTGCATGGACAAAGCAATACGGAAAGAGGTTTATATATTGGAACGGGATGGAGCCGAGGCTGTGTTTGACGGAGATGGAGTTGGTGAAGGAAGTATTGGGGAAGGGCAGCAATGTGTGTGGGAGGTCATGGATGCAAAGACAAGGGACCAAACACTTCATAGGGAAGGGATTGTTGATGGCAAATGGAGAAGCTTGGCATCATCAACGTCACCTGATTTCACCTGCGTTTAGAGGGGAAAGGCTCAAGGTTCATATTCAAACCTGA

Coding sequence (CDS)

ATGGCATATGATACTCTCTCTTTCTACTGGCTTACCCCTAGAAGAATCAGGAAGAGGATGGCCGAGCAAGGCGTTTATGGCCCCGACCCACGACCCTTCACTGGAAACATCCTCGATGTCGTAGAGCTTGTGAAGAAATCCACGGCTAACGACATGAGCTCCATCAGTCATGACATCGTTGATCGTTTGTTACCCTATTACTCTGCATGGACAAAGCAATACGGAAAGAGGTTTATATATTGGAACGGGATGGAGCCGAGGCTGTGTTTGACGGAGATGGAGTTGGTGAAGGAAGTATTGGGGAAGGGCAGCAATGTGTGTGGGAGGTCATGGATGCAAAGACAAGGGACCAAACACTTCATAGGGAAGGGATTGTTGATGGCAAATGGAGAAGCTTGGCATCATCAACGTCACCTGATTTCACCTGCGTTTAGAGGGGAAAGGCTCAAGGTTCATATTCAAACCTGA

Protein sequence

MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIGKGLLMANGEAWHHQRHLISPAFRGERLKVHIQT

Homology

[Close]

BLAST of Csor.00g194700 vs. ExPASy Swiss-Prot
Match: Q9FF18 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.1e-59
Identity = 102/148 (68.92%), Postives = 122/148 (82.43%), Query Frame = 0


Query: 3   YDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDR 62
           YDT+S YWLTPRRI+K M +QGV GP PRP TGNIL++  +V +S + D  SI HDIV R
Sbjct: 22  YDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQSASKDCDSIHHDIVGR 81

Query: 63  LLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIG 122
           LLP+Y AW+KQYGKRFI WNG +PRLCLTE EL+KE+L K + V GRSW+Q+QGTK+FIG
Sbjct: 82  LLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRSWLQQQGTKNFIG 141

Query: 123 KGLLMANGEAWHHQRHLISPAFRGERLK 151
           +GLLMANG+ WHHQRHL +PAF GERLK
Sbjct: 142 RGLLMANGQDWHHQRHLAAPAFTGERLK 169

[Close]

BLAST of Csor.00g194700 vs. ExPASy Swiss-Prot
Match: Q9ZW95 (Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 6.3e-55
Identity = 94/148 (63.51%), Postives = 120/148 (81.08%), Query Frame = 0


Query: 3   YDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDR 62
           YD++  Y+LTPRRI+K M  QG+ GP PR  TGNI+D+ +++  S +ND SSI H+IV R
Sbjct: 23  YDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSASNDCSSIHHNIVPR 82

Query: 63  LLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIG 122
           LLP+Y +W+KQYGKRFI WNG EPRLCLTE E++KE+L K + V G+SW+Q+QGTK FIG
Sbjct: 83  LLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQQGTKGFIG 142

Query: 123 KGLLMANGEAWHHQRHLISPAFRGERLK 151
           +GLLMANGEAWHHQRH+ +PAF  +RLK
Sbjct: 143 RGLLMANGEAWHHQRHMAAPAFTRDRLK 170

[Close]

BLAST of Csor.00g194700 vs. ExPASy Swiss-Prot
Match: Q9ASR3 (Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 5.8e-24
Identity = 49/139 (35.25%), Postives = 82/139 (58.99%), Query Frame = 0


Query: 13  PRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDRLLPYYSAWTK 72
           P  + +R+  QG+ GP+ R F GN+ ++ ++ ++S  + +   S+DI  R+LP+Y  W  
Sbjct: 31  PFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKRESHLSILDPSSNDIFPRILPHYQKWMS 90

Query: 73  QYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIGKGLLMANGEA 132
           QYG+ F+YWNG EPR+C+++ EL K +L        +S  + +  K    KGL+   G  
Sbjct: 91  QYGETFLYWNGTEPRICISDPELAKTMLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGAD 150

Query: 133 WHHQRHLISPAFRGERLKV 152
           W   R +++PAF  +RLK+
Sbjct: 151 WVRHRRILNPAFSIDRLKI 169

[Close]

BLAST of Csor.00g194700 vs. ExPASy Swiss-Prot
Match: Q8LIF2 (Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 4.6e-21
Identity = 53/143 (37.06%), Postives = 80/143 (55.94%), Query Frame = 0


Query: 10  WLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMS-SISHDIVDRLLPYYS 69
           W  PRR+ +  A QGV GP  R F G+ +++V L+  + +  M    SHDI+ R+LP+Y 
Sbjct: 40  WWRPRRVERHFAAQGVRGPGYRFFVGSSIELVRLMVDAASRPMEPPTSHDILPRVLPFYH 99

Query: 70  AWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIGKGLLMA 129
            W K YG   + W G  PRL ++E EL++EVL   ++   R +        F G GL   
Sbjct: 100 HWRKLYGPMHLIWFGRTPRLVVSEPELIREVLLTRADHFDR-YEAHPMICQFEGYGLSNL 159

Query: 130 NGEAWHHQRHLISPAFRGERLKV 152
           +GE W  +R +++PAF  E L++
Sbjct: 160 HGERWARRRRVLTPAFHTENLRM 181

[Close]

BLAST of Csor.00g194700 vs. ExPASy Swiss-Prot
Match: F4IK45 (Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1)

HSP 1 Score: 102.1 bits (253), Expect = 6.0e-21
Identity = 49/145 (33.79%), Postives = 81/145 (55.86%), Query Frame = 0


Query: 10  WLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDRLLPYYSA 69
           W+  RR +K    QG+ GP  R   GN+ ++ ++  ++    +   S+DIV R+LP+   
Sbjct: 89  WMLSRRFKK----QGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQ 148

Query: 70  WTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIGKGLLMAN 129
           W  QYG+ F+YW G +PRLC+++ EL K++L        +S  + +  K   G GL+  N
Sbjct: 149 WKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILK-LSGNGLIFVN 208

Query: 130 GEAWHHQRHLISPAFRGERLKVHIQ 155
           G  W   R +++PAF  ++LK+  Q
Sbjct: 209 GLDWVRHRRILNPAFSMDKLKLMTQ 228

[Close]

BLAST of Csor.00g194700 vs. NCBI nr
Match: KAG6607036.1 (Cytokinin hydroxylase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 331 bits (848), Expect = 1.18e-114
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV
Sbjct: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF
Sbjct: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLKVHIQT 155
           IGKGLLMANGEAWHHQRHLISPAFRGERLKVHIQT
Sbjct: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLKVHIQT 155

[Close]

BLAST of Csor.00g194700 vs. NCBI nr
Match: XP_023524594.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 309 bits (791), Expect = 2.52e-104
Identity = 144/150 (96.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           +AYDTLSFYWLTPRRIRKRMAEQGVYGP+PRPFTGNILDVVELVK+STANDMSSISHDIV
Sbjct: 21  IAYDTLSFYWLTPRRIRKRMAEQGVYGPNPRPFTGNILDVVELVKESTANDMSSISHDIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGK SNVCGRSWMQRQGTKHF
Sbjct: 81  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKSSNVCGRSWMQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IG GLLMANGE WHHQRHLISPAFRGERLK
Sbjct: 141 IGNGLLMANGENWHHQRHLISPAFRGERLK 170

[Close]

BLAST of Csor.00g194700 vs. NCBI nr
Match: XP_022948950.1 (cytokinin hydroxylase-like [Cucurbita moschata])

HSP 1 Score: 315 bits (806), Expect = 3.72e-103
Identity = 147/150 (98.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV
Sbjct: 21  MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLPYYS WTKQYGKRFIYWNGM+ RLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF
Sbjct: 81  DRLLPYYSTWTKQYGKRFIYWNGMDSRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IGKGLLMANGEAWHHQRHLISPAFRGERLK
Sbjct: 141 IGKGLLMANGEAWHHQRHLISPAFRGERLK 170

[Close]

BLAST of Csor.00g194700 vs. NCBI nr
Match: XP_023523398.1 (cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 309 bits (791), Expect = 8.29e-101
Identity = 144/150 (96.00%), Postives = 147/150 (98.00%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           +AYDTLSFYWLTPRRIRKRMAEQGVYGP+PRPFTGNILDVVELVK+STANDMSSISHDIV
Sbjct: 21  IAYDTLSFYWLTPRRIRKRMAEQGVYGPNPRPFTGNILDVVELVKESTANDMSSISHDIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGK SNVCGRSWMQRQGTKHF
Sbjct: 81  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKSSNVCGRSWMQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IG GLLMANGE WHHQRHLISPAFRGERLK
Sbjct: 141 IGNGLLMANGENWHHQRHLISPAFRGERLK 170

[Close]

BLAST of Csor.00g194700 vs. NCBI nr
Match: XP_022997665.1 (cytokinin hydroxylase-like [Cucurbita maxima])

HSP 1 Score: 306 bits (785), Expect = 5.46e-100
Identity = 141/150 (94.00%), Postives = 146/150 (97.33%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           +AYDTLSFYWLTPRRIRKRMA+QGVYGPDPRPFTGNILDVVELVK+S ANDMSS SHDIV
Sbjct: 21  IAYDTLSFYWLTPRRIRKRMAKQGVYGPDPRPFTGNILDVVELVKESMANDMSSTSHDIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKE+LGK SNVCGRSWMQRQGTKHF
Sbjct: 81  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEILGKSSNVCGRSWMQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IG GLLMANGEAWHHQRHL+SPAFRGERLK
Sbjct: 141 IGNGLLMANGEAWHHQRHLVSPAFRGERLK 170

[Close]

BLAST of Csor.00g194700 vs. ExPASy TrEMBL
Match: A0A6J1GBC3 (cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111452447 PE=3 SV=1)

HSP 1 Score: 315 bits (806), Expect = 1.80e-103
Identity = 147/150 (98.00%), Postives = 148/150 (98.67%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV
Sbjct: 21  MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLPYYS WTKQYGKRFIYWNGM+ RLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF
Sbjct: 81  DRLLPYYSTWTKQYGKRFIYWNGMDSRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IGKGLLMANGEAWHHQRHLISPAFRGERLK
Sbjct: 141 IGKGLLMANGEAWHHQRHLISPAFRGERLK 170

[Close]

BLAST of Csor.00g194700 vs. ExPASy TrEMBL
Match: A0A6J1KAH1 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111492562 PE=3 SV=1)

HSP 1 Score: 306 bits (785), Expect = 2.64e-100
Identity = 141/150 (94.00%), Postives = 146/150 (97.33%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           +AYDTLSFYWLTPRRIRKRMA+QGVYGPDPRPFTGNILDVVELVK+S ANDMSS SHDIV
Sbjct: 21  IAYDTLSFYWLTPRRIRKRMAKQGVYGPDPRPFTGNILDVVELVKESMANDMSSTSHDIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKE+LGK SNVCGRSWMQRQGTKHF
Sbjct: 81  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEILGKSSNVCGRSWMQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IG GLLMANGEAWHHQRHL+SPAFRGERLK
Sbjct: 141 IGNGLLMANGEAWHHQRHLVSPAFRGERLK 170

[Close]

BLAST of Csor.00g194700 vs. ExPASy TrEMBL
Match: A0A1S3CGC0 (cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1)

HSP 1 Score: 258 bits (660), Expect = 1.95e-81
Identity = 115/150 (76.67%), Postives = 132/150 (88.00%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           +AYDT+S YWLTPRRIRK+M +QGV GP PRP  GNILDV  L+ KSTA DMSSI H+IV
Sbjct: 21  IAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAALLAKSTAKDMSSIDHNIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLP+Y+ WTKQYGKRFIYWNGMEPRLCL E EL+KEVL K SNVCGRSW+QRQGTKHF
Sbjct: 81  DRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKNSNVCGRSWLQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IGKGLLMANGE W+HQRH+++P+F G++LK
Sbjct: 141 IGKGLLMANGEDWYHQRHIVAPSFMGDKLK 170

[Close]

BLAST of Csor.00g194700 vs. ExPASy TrEMBL
Match: A0A0A0L6Y6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1)

HSP 1 Score: 258 bits (659), Expect = 2.75e-81
Identity = 115/150 (76.67%), Postives = 132/150 (88.00%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           +AYDT+S YWLTPRRIRK+M +QGV GP PRP  GNILDV  L+ KSTA DMSSI H+IV
Sbjct: 21  IAYDTISCYWLTPRRIRKKMEKQGVRGPVPRPLVGNILDVAALLGKSTAKDMSSIDHNIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLP+Y+ WTKQYGKRFIYWNGMEPRLCL E EL+KEVL K SNVCGRSW+QRQGTKHF
Sbjct: 81  DRLLPHYTTWTKQYGKRFIYWNGMEPRLCLAETELIKEVLSKNSNVCGRSWLQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IGKGLLMANGE W+HQRH+++P+F G++LK
Sbjct: 141 IGKGLLMANGEDWYHQRHIVAPSFMGDKLK 170

[Close]

BLAST of Csor.00g194700 vs. ExPASy TrEMBL
Match: A0A6J1J2Y1 (cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1)

HSP 1 Score: 254 bits (648), Expect = 1.05e-79
Identity = 116/150 (77.33%), Postives = 131/150 (87.33%), Query Frame = 0


Query: 1   MAYDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIV 60
           ++YDT SFY LTPRRIRK M +QGV GP PR   GNILDVV LV KSTANDMSSI HDIV
Sbjct: 21  ISYDTFSFYCLTPRRIRKTMEQQGVRGPKPRLLIGNILDVVGLVAKSTANDMSSIDHDIV 80

Query: 61  DRLLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHF 120
           DRLLP+Y+ WTKQYGKRFIYWNG EPRLCL E EL+KE+LGK S+VCGRSW+QRQGTKHF
Sbjct: 81  DRLLPHYTIWTKQYGKRFIYWNGTEPRLCLAETELIKELLGKKSSVCGRSWLQRQGTKHF 140

Query: 121 IGKGLLMANGEAWHHQRHLISPAFRGERLK 150
           IGKGLLMANGE W+HQRH+++PAF G++LK
Sbjct: 141 IGKGLLMANGEDWYHQRHIVAPAFMGDKLK 170

[Close]

BLAST of Csor.00g194700 vs. TAIR 10
Match: AT5G38450.1 (cytochrome P450, family 735, subfamily A, polypeptide 1 )

HSP 1 Score: 230.7 bits (587), Expect = 7.9e-61
Identity = 102/148 (68.92%), Postives = 122/148 (82.43%), Query Frame = 0


Query: 3   YDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDR 62
           YDT+S YWLTPRRI+K M +QGV GP PRP TGNIL++  +V +S + D  SI HDIV R
Sbjct: 22  YDTISCYWLTPRRIKKIMEQQGVTGPKPRPLTGNILEISAMVSQSASKDCDSIHHDIVGR 81

Query: 63  LLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIG 122
           LLP+Y AW+KQYGKRFI WNG +PRLCLTE EL+KE+L K + V GRSW+Q+QGTK+FIG
Sbjct: 82  LLPHYVAWSKQYGKRFIVWNGTDPRLCLTETELIKELLMKHNGVSGRSWLQQQGTKNFIG 141

Query: 123 KGLLMANGEAWHHQRHLISPAFRGERLK 151
           +GLLMANG+ WHHQRHL +PAF GERLK
Sbjct: 142 RGLLMANGQDWHHQRHLAAPAFTGERLK 169

[Close]

BLAST of Csor.00g194700 vs. TAIR 10
Match: AT1G67110.1 (cytochrome P450, family 735, subfamily A, polypeptide 2 )

HSP 1 Score: 214.9 bits (546), Expect = 4.5e-56
Identity = 94/148 (63.51%), Postives = 120/148 (81.08%), Query Frame = 0


Query: 3   YDTLSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDR 62
           YD++  Y+LTPRRI+K M  QG+ GP PR  TGNI+D+ +++  S +ND SSI H+IV R
Sbjct: 23  YDSICCYFLTPRRIKKFMERQGITGPKPRLLTGNIIDISKMLSHSASNDCSSIHHNIVPR 82

Query: 63  LLPYYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIG 122
           LLP+Y +W+KQYGKRFI WNG EPRLCLTE E++KE+L K + V G+SW+Q+QGTK FIG
Sbjct: 83  LLPHYVSWSKQYGKRFIMWNGTEPRLCLTETEMIKELLTKHNPVTGKSWLQQQGTKGFIG 142

Query: 123 KGLLMANGEAWHHQRHLISPAFRGERLK 151
           +GLLMANGEAWHHQRH+ +PAF  +RLK
Sbjct: 143 RGLLMANGEAWHHQRHMAAPAFTRDRLK 170

[Close]

BLAST of Csor.00g194700 vs. TAIR 10
Match: AT2G46960.2 (cytochrome P450, family 709, subfamily B, polypeptide 1 )

HSP 1 Score: 112.1 bits (279), Expect = 4.1e-25
Identity = 49/139 (35.25%), Postives = 82/139 (58.99%), Query Frame = 0


Query: 13  PRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDRLLPYYSAWTK 72
           P  + +R+  QG+ GP+ R F GN+ ++ ++ ++S  + +   S+DI  R+LP+Y  W  
Sbjct: 31  PFVLTRRLKNQGISGPNYRIFYGNLSEIKKMKRESHLSILDPSSNDIFPRILPHYQKWMS 90

Query: 73  QYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIGKGLLMANGEA 132
           QYG+ F+YWNG EPR+C+++ EL K +L        +S  + +  K    KGL+   G  
Sbjct: 91  QYGETFLYWNGTEPRICISDPELAKTMLSNKLGFFVKSKARPEAVKLVGSKGLVFIEGAD 150

Query: 133 WHHQRHLISPAFRGERLKV 152
           W   R +++PAF  +RLK+
Sbjct: 151 WVRHRRILNPAFSIDRLKI 169

[Close]

BLAST of Csor.00g194700 vs. TAIR 10
Match: AT2G46950.1 (cytochrome P450, family 709, subfamily B, polypeptide 2 )

HSP 1 Score: 102.1 bits (253), Expect = 4.2e-22
Identity = 49/145 (33.79%), Postives = 81/145 (55.86%), Query Frame = 0


Query: 10  WLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDRLLPYYSA 69
           W+  RR +K    QG+ GP  R   GN+ ++ ++  ++    +   S+DIV R+LP+   
Sbjct: 89  WMLSRRFKK----QGISGPKYRILYGNLREIRKMKNEAKLMVLDPNSNDIVPRVLPHLQQ 148

Query: 70  WTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIGKGLLMAN 129
           W  QYG+ F+YW G +PRLC+++ EL K++L        +S  + +  K   G GL+  N
Sbjct: 149 WKSQYGETFLYWQGTDPRLCISDHELAKQILSNKFVFFSKSKTKPEILK-LSGNGLIFVN 208

Query: 130 GEAWHHQRHLISPAFRGERLKVHIQ 155
           G  W   R +++PAF  ++LK+  Q
Sbjct: 209 GLDWVRHRRILNPAFSMDKLKLMTQ 228

[Close]

BLAST of Csor.00g194700 vs. TAIR 10
Match: AT2G26710.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 100.1 bits (248), Expect = 1.6e-21
Identity = 50/146 (34.25%), Postives = 78/146 (53.42%), Query Frame = 0


Query: 6   LSFYWLTPRRIRKRMAEQGVYGPDPRPFTGNILDVVELVKKSTANDMSSISHDIVDRLLP 65
           +S  W  PR+I +  ++QG+ GP    F GN+ ++V ++ K++++ M   SH+I+ R+L 
Sbjct: 28  MSLLWWRPRKIEEHFSKQGIRGPPYHFFIGNVKELVGMMLKASSHPM-PFSHNILPRVLS 87

Query: 66  YYSAWTKQYGKRFIYWNGMEPRLCLTEMELVKEVLGKGSNVCGRSWMQRQGTKHFIGKGL 125
           +Y  W K YG  F+ W G   RL + + +L++E+  K               K   G GL
Sbjct: 88  FYHHWRKIYGATFLVWFGPTFRLTVADPDLIREIFSKSEFY--EKNEAHPLVKQLEGDGL 147

Query: 126 LMANGEAWHHQRHLISPAFRGERLKV 152
           L   GE W H R +ISP F  E LK+
Sbjct: 148 LSLKGEKWAHHRKIISPTFHMENLKL 170

The following BLAST results are available for this feature:

BLAST of Csor.00g194700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FF18	1.1e-59	68.92	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A1 PE=1 SV=1	[more]
Q9ZW95	6.3e-55	63.51	Cytokinin hydroxylase OS=Arabidopsis thaliana OX=3702 GN=CYP735A2 PE=1 SV=1	[more]
Q9ASR3	5.8e-24	35.25	Cytochrome P450 709B1 OS=Arabidopsis thaliana OX=3702 GN=CYP709B1 PE=2 SV=1	[more]
Q8LIF2	4.6e-21	37.06	Cytochrome P450 734A5 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP734A5 PE=2 ...	[more]
F4IK45	6.0e-21	33.79	Cytochrome P450 709B2 OS=Arabidopsis thaliana OX=3702 GN=CYP709B2 PE=2 SV=1	[more]

BLAST of Csor.00g194700 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6607036.1	1.18e-114	100.00	Cytokinin hydroxylase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023524594.1	2.52e-104	96.00	cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022948950.1	3.72e-103	98.00	cytokinin hydroxylase-like [Cucurbita moschata]	[more]
XP_023523398.1	8.29e-101	96.00	cytokinin hydroxylase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022997665.1	5.46e-100	94.00	cytokinin hydroxylase-like [Cucurbita maxima]	[more]

BLAST of Csor.00g194700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GBC3	1.80e-103	98.00	cytokinin hydroxylase-like OS=Cucurbita moschata OX=3662 GN=LOC111452447 PE=3 SV...	[more]
A0A6J1KAH1	2.64e-100	94.00	cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111492562 PE=3 SV=1	[more]
A0A1S3CGC0	1.95e-81	76.67	cytokinin hydroxylase-like OS=Cucumis melo OX=3656 GN=LOC103500156 PE=3 SV=1	[more]
A0A0A0L6Y6	2.75e-81	76.67	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G271920 PE=3 SV=1	[more]
A0A6J1J2Y1	1.05e-79	77.33	cytokinin hydroxylase-like OS=Cucurbita maxima OX=3661 GN=LOC111482203 PE=3 SV=1	[more]

BLAST of Csor.00g194700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G38450.1	7.9e-61	68.92	cytochrome P450, family 735, subfamily A, polypeptide 1	[more]
AT1G67110.1	4.5e-56	63.51	cytochrome P450, family 735, subfamily A, polypeptide 2	[more]
AT2G46960.2	4.1e-25	35.25	cytochrome P450, family 709, subfamily B, polypeptide 1	[more]
AT2G46950.1	4.2e-22	33.79	cytochrome P450, family 709, subfamily B, polypeptide 2	[more]
AT2G26710.1	1.6e-21	34.25	Cytochrome P450 superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001128	Cytochrome P450	PFAM	PF00067	p450	coord: 66..147 e-value: 7.4E-10 score: 38.1
IPR036396	Cytochrome P450 superfamily	GENE3D	1.10.630.10	Cytochrome P450	coord: 8..155 e-value: 3.2E-19 score: 70.8
IPR036396	Cytochrome P450 superfamily	SUPERFAMILY	48264	Cytochrome P450	coord: 24..152
None	No IPR available	PANTHER	PTHR24282	CYTOCHROME P450 FAMILY MEMBER	coord: 2..151
None	No IPR available	PANTHER	PTHR24282:SF186	CYTOCHROME P450 FAMILY PROTEIN	coord: 2..151

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g194700.m01	Csor.00g194700.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0004497	monooxygenase activity
molecular_function	GO:0016705	oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen