Csor.00g159670 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g159670
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
DescriptionEukaryotic aspartyl protease family protein
LocationCsor_Chr15: 11028361 .. 11029740 (-)
RNA-Seq ExpressionCsor.00g159670
SyntenyCsor.00g159670
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSsinglepolypeptidestart_codonstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAACAACAATGCTCTCCGTTTCCCCTTTCTTCCATTTCATTCTTCTCCTCTTCTCTCTCGCCGATCTCCTCAACGCCGCCGCCGCCGCCGACTACCTGAAGCTCCCATTGCTCCACAAGAACCCCTTCTCCTCCCCTTCCCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTGCCCTCCGCCGCCGCCCTAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCCTCCACCGGCTCCGGCCAGTACTTCGTCGACCTCCGCATTGGCACTCCTCCCCAGAGCCTCCTCCTCGTCGCCGATACTGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCTTTCCTCCCCCGTCACTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTCCCCCACGCTCCTCCCCACCTCTGTAACCACACGCGCCTCCACTCCCCCTGCCGCTTCCTCTACACTTACGCCGACGGCTCCACCTCCTCCGGCTTCTTCTCCAAGGAAACCACGACGTTGAAGACCCTCTCTGGCTCCGAGACCCGTCTTAAAGATCTGTCGTTCGGGTGCGGCTTTCGGATCTCGGGTCCGAGCGTTTCGGGCGCCCAATTTAATGGAGCACGTGGTGTCATGGGATTGGGCAGAGGTCCGATCTCCTTCTCCACTCAACTCGGCCGCCGATTTGGCAATAAATTTTCCTATTGTCTTATGGATTACACACTCTCTCCGCCGCCCACCAGCTACCTAATGATCGGTGGCGGCCTCCGTAGCCTCCCCGTGACCAATGCCACAAAAATCAGCTACACCCCATTGCTAATTAACCCTCTGTCACCAACATTCTACTACATTGCCGTCAAGAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCGATCGTGTGGGCCATCAACGAGCAAGGAAACGGCGGGACGGTGGTGGATTCGGGAACGACGCTGACGTATCTGGCAGAGGAGGCGTACAAGGAGGTGCTGAAGGCGATGAGACAGCGAGTGAAGCTACCGGCTGCAGCAGAGTTGACTCCGGGATTCGATCTGTGCGTGAACGTATCAAACGAATCGCAGCGGCCGAGTCTACCACGAGTGAGATTCCAGCTGGGAAATGGTGCAGTGTTTGCGCCGCCGGCGAGGAACTACTTTCTGGATACGGAGGAGGGGGTGATGTGCTTGGCGATCCGAGCAGTGGAAGGCGGAAATGGGTTCTCGGTGATTGGGAATCTGATGCAACAAGGATTCCTGCTGGAGTTCGACAAGGAGGCGTCGAGGCTGGGCTTCTCAAGGCGGGGCTGCGGCCTTCCTTGA

mRNA sequence

ATGCAAACAACAATGCTCTCCGTTTCCCCTTTCTTCCATTTCATTCTTCTCCTCTTCTCTCTCGCCGATCTCCTCAACGCCGCCGCCGCCGCCGACTACCTGAAGCTCCCATTGCTCCACAAGAACCCCTTCTCCTCCCCTTCCCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTGCCCTCCGCCGCCGCCCTAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCCTCCACCGGCTCCGGCCAGTACTTCGTCGACCTCCGCATTGGCACTCCTCCCCAGAGCCTCCTCCTCGTCGCCGATACTGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCTTTCCTCCCCCGTCACTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTCCCCCACGCTCCTCCCCACCTCTGTAACCACACGCGCCTCCACTCCCCCTGCCGCTTCCTCTACACTTACGCCGACGGCTCCACCTCCTCCGGCTTCTTCTCCAAGGAAACCACGACGTTGAAGACCCTCTCTGGCTCCGAGACCCGTCTTAAAGATCTGTCGTTCGGGTGCGGCTTTCGGATCTCGGGTCCGAGCGTTTCGGGCGCCCAATTTAATGGAGCACGTGGTGTCATGGGATTGGGCAGAGGTCCGATCTCCTTCTCCACTCAACTCGGCCGCCGATTTGGCAATAAATTTTCCTATTGTCTTATGGATTACACACTCTCTCCGCCGCCCACCAGCTACCTAATGATCGGTGGCGGCCTCCGTAGCCTCCCCGTGACCAATGCCACAAAAATCAGCTACACCCCATTGCTAATTAACCCTCTGTCACCAACATTCTACTACATTGCCGTCAAGAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCGATCGTGTGGGCCATCAACGAGCAAGGAAACGGCGGGACGGTGGTGGATTCGGGAACGACGCTGACGTATCTGGCAGAGGAGGCGTACAAGGAGGTGCTGAAGGCGATGAGACAGCGAGTGAAGCTACCGGCTGCAGCAGAGTTGACTCCGGGATTCGATCTGTGCGTGAACGTATCAAACGAATCGCAGCGGCCGAGTCTACCACGAGTGAGATTCCAGCTGGGAAATGGTGCAGTGTTTGCGCCGCCGGCGAGGAACTACTTTCTGGATACGGAGGAGGGGGTGATGTGCTTGGCGATCCGAGCAGTGGAAGGCGGAAATGGGTTCTCGGTGATTGGGAATCTGATGCAACAAGGATTCCTGCTGGAGTTCGACAAGGAGGCGTCGAGGCTGGGCTTCTCAAGGCGGGGCTGCGGCCTTCCTTGA

Coding sequence (CDS)

ATGCAAACAACAATGCTCTCCGTTTCCCCTTTCTTCCATTTCATTCTTCTCCTCTTCTCTCTCGCCGATCTCCTCAACGCCGCCGCCGCCGCCGACTACCTGAAGCTCCCATTGCTCCACAAGAACCCCTTCTCCTCCCCTTCCCAAGCCCTCTCCTCCGACACCCACCGCCTCTCCCTCCTCTTCTCTGCCCTCCGCCGCCGCCCTAACCCCACTCTCAAATCCCCTCTCATCTCCGGCGCCTCCACCGGCTCCGGCCAGTACTTCGTCGACCTCCGCATTGGCACTCCTCCCCAGAGCCTCCTCCTCGTCGCCGATACTGGCAGCGACCTCGTCTGGGTCAAATGCTCCGCCTGCCGCAACTGCTCCCACCACCCTCCCTCCTCTGCTTTCCTCCCCCGTCACTCCTCCTCCTTCTCCCCTTTCCACTGCTTCGACCCCCACTGCCGCCTCCTCCCCCACGCTCCTCCCCACCTCTGTAACCACACGCGCCTCCACTCCCCCTGCCGCTTCCTCTACACTTACGCCGACGGCTCCACCTCCTCCGGCTTCTTCTCCAAGGAAACCACGACGTTGAAGACCCTCTCTGGCTCCGAGACCCGTCTTAAAGATCTGTCGTTCGGGTGCGGCTTTCGGATCTCGGGTCCGAGCGTTTCGGGCGCCCAATTTAATGGAGCACGTGGTGTCATGGGATTGGGCAGAGGTCCGATCTCCTTCTCCACTCAACTCGGCCGCCGATTTGGCAATAAATTTTCCTATTGTCTTATGGATTACACACTCTCTCCGCCGCCCACCAGCTACCTAATGATCGGTGGCGGCCTCCGTAGCCTCCCCGTGACCAATGCCACAAAAATCAGCTACACCCCATTGCTAATTAACCCTCTGTCACCAACATTCTACTACATTGCCGTCAAGAGCATCACCGTCGACGGCGTGAAATTACCCATCAACCCGATCGTGTGGGCCATCAACGAGCAAGGAAACGGCGGGACGGTGGTGGATTCGGGAACGACGCTGACGTATCTGGCAGAGGAGGCGTACAAGGAGGTGCTGAAGGCGATGAGACAGCGAGTGAAGCTACCGGCTGCAGCAGAGTTGACTCCGGGATTCGATCTGTGCGTGAACGTATCAAACGAATCGCAGCGGCCGAGTCTACCACGAGTGAGATTCCAGCTGGGAAATGGTGCAGTGTTTGCGCCGCCGGCGAGGAACTACTTTCTGGATACGGAGGAGGGGGTGATGTGCTTGGCGATCCGAGCAGTGGAAGGCGGAAATGGGTTCTCGGTGATTGGGAATCTGATGCAACAAGGATTCCTGCTGGAGTTCGACAAGGAGGCGTCGAGGCTGGGCTTCTCAAGGCGGGGCTGCGGCCTTCCTTGA

Protein sequence

MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Homology
[Close]
BLAST of Csor.00g159670 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 6.0e-61
Identity = 151/436 (34.63%), Postives = 224/436 (51.38%), Query Frame = 0 

Query: 32  DYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPNPTL---------KSPLISGAS 91
           D++     +K P    S  L  D+ R+  + +   + P   +          S ++SG S
Sbjct: 77  DHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLS 136

Query: 92  TGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPF 151
            GSG+YF  L +GTP + + +V DTGSD+VW++C+ CR C +      F PR S +++  
Sbjct: 137 QGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPRKSKTYATI 196

Query: 152 HCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLSGSETRL 211
            C  PHCR L  A    CN  R    C +  +Y DGS + G FS ET T +       R+
Sbjct: 197 PCSSPHCRRLDSAG---CNTRR--KTCLYQVSYGDGSFTVGDFSTETLTFR-----RNRV 256

Query: 212 KDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSP 271
           K ++ GCG    G       F GA G++GLG+G +SF  Q G RF  KFSYCL+D + S 
Sbjct: 257 KGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASS 316

Query: 272 PPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLP-INPIVW 331
            P+S +     +  +         +TPLL NP   TFYY+ +  I+V G ++P +   ++
Sbjct: 317 KPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLF 376

Query: 332 AINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQ 391
            +++ GNGG ++DSGT++T L   AY  +  A R   K    A     FD C ++SN ++
Sbjct: 377 KLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNE 436

Query: 392 RPSLPRVRFQLGNGAVFAPPARNYFLDTE-EGVMCLAIRAVEGGNGFSVIGNLMQQGFLL 451
              +P V      GA  + PA NY +  +  G  C A     G  G S+IGN+ QQGF +
Sbjct: 437 -VKVPTVVLHF-RGADVSLPATNYLIPVDTNGKFCFAFAGTMG--GLSIIGNIQQQGFRV 484

Query: 452 EFDKEASRLGFSRRGC 457
            +D  +SR+GF+  GC
Sbjct: 497 VYDLASSRVGFAPGGC 484

[Close]
BLAST of Csor.00g159670 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 214.9 bits (546), Expect = 1.9e-54
Identity = 130/374 (34.76%), Postives = 197/374 (52.67%), Query Frame = 0 

Query: 84  GSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFH 143
           G G+Y +++ IGTP  S   + DTGSDL+W +C  C  C    P+  F P+ SSSFS   
Sbjct: 92  GDGEYLMNVAIGTPDSSFSAIMDTGSDLIWTQCEPCTQC-FSQPTPIFNPQDSSSFSTLP 151

Query: 144 CFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLSGSETRLK 203
           C   +C+ L   P   CN    ++ C++ Y Y DGST+ G+ + ET T +T S     + 
Sbjct: 152 CESQYCQDL---PSETCN----NNECQYTYGYGDGSTTQGYMATETFTFETSS-----VP 211

Query: 204 DLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSPP 263
           +++FGCG    G      Q NGA G++G+G GP+S  +QLG     +FSYC+  Y  S P
Sbjct: 212 NIAFGCGEDNQG----FGQGNGA-GLIGMGWGPLSLPSQLG---VGQFSYCMTSYGSSSP 271

Query: 264 PTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPINPIVWAI 323
            T  L +G     +P  + +    T L+ + L+PT+YYI ++ ITV G  L I    + +
Sbjct: 272 ST--LALGSAASGVPEGSPS----TTLIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQL 331

Query: 324 NEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQRP 383
            + G GG ++DSGTTLTYL ++AY  V +A   ++ LP   E + G   C    ++    
Sbjct: 332 QDDGTGGMIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTV 391

Query: 384 SLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEFD 443
            +P +  Q  +G V     +N  +   EGV+CLA+ +     G S+ GN+ QQ   + +D
Sbjct: 392 QVPEISMQF-DGGVLNLGEQNILISPAEGVICLAMGS-SSQLGISIFGNIQQQETQVLYD 436

Query: 444 KEASRLGFSRRGCG 458
            +   + F    CG
Sbjct: 452 LQNLAVSFVPTQCG 436

[Close]
BLAST of Csor.00g159670 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 208.0 bits (528), Expect = 2.3e-52
Identity = 129/375 (34.40%), Postives = 192/375 (51.20%), Query Frame = 0 

Query: 84  GSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPFH 143
           G G+Y ++L IGTP Q    + DTGSDL+W +C  C  C  +  +  F P+ SSSFS   
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQCQPCTQC-FNQSTPIFNPQGSSSFSTLP 150

Query: 144 CFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLSGSETRLK 203
           C    C+ L  + P   N+      C++ Y Y DGS + G    ET T  ++S     + 
Sbjct: 151 CSSQLCQAL--SSPTCSNNF-----CQYTYGYGDGSETQGSMGTETLTFGSVS-----IP 210

Query: 204 DLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSPP 263
           +++FGCG    G      Q NGA G++G+GRGP+S  +QL      KFSYC+     S P
Sbjct: 211 NITFGCGENNQG----FGQGNGA-GLVGMGRGPLSLPSQLD---VTKFSYCMTPIGSSTP 270

Query: 264 PTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPINPIVWAI 323
             S L++G    S+   +      T L+ +   PTFYYI +  ++V   +LPI+P  +A+
Sbjct: 271 --SNLLLGSLANSVTAGSPN----TTLIQSSQIPTFYYITLNGLSVGSTRLPIDPSAFAL 330

Query: 324 N-EQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQR 383
           N   G GG ++DSGTTLTY    AY+ V +    ++ LP     + GFDLC    ++   
Sbjct: 331 NSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVVNGSSSGFDLCFQTPSDPSN 390

Query: 384 PSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGGNGFSVIGNLMQQGFLLEF 443
             +P       +G     P+ NYF+    G++CLA+ +     G S+ GN+ QQ  L+ +
Sbjct: 391 LQIPTFVMHF-DGGDLELPSENYFISPSNGLICLAMGS--SSQGMSIFGNIQQQNMLVVY 435

Query: 444 DKEASRLGFSRRGCG 458
           D   S + F+   CG
Sbjct: 451 DTGNSVVSFASAQCG 435

[Close]
BLAST of Csor.00g159670 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 204.1 bits (518), Expect = 3.3e-51
Identity = 154/465 (33.12%), Postives = 226/465 (48.60%), Query Frame = 0 

Query: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKN-----PFSSPSQALSSDT 60
           MQ T+LS        LLL ++AD +   +    ++L L H++     P S     + +D 
Sbjct: 23  MQKTLLSC---LITTLLLITVADSMKDTS----VRLKLAHRDTLLPKPLSRIEDVIGADQ 82

Query: 61  HRLSLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVK 120
            R SL+  + +R     +K  L SG   G+ QYF ++R+GTP +   +V DTGS+L WV 
Sbjct: 83  KRHSLI--SRKRNSTVGVKMDLGSGIDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVN 142

Query: 121 CSACRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTY 180
           C              F    S SF    C    C++       L       +PC + Y Y
Sbjct: 143 CR--YRARGKDNRRVFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRY 202

Query: 181 ADGSTSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRG 240
           ADGS + G F+KET T+   +G   RL     GC       S +G  F GA GV+GL   
Sbjct: 203 ADGSAAQGVFAKETITVGLTNGRMARLPGHLIGC-----SSSFTGQSFQGADGVLGLAFS 262

Query: 241 PISFSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISY---TPLLI 300
             SF++     +G KFSYCL+D+  +   ++YL+ G        + +TK ++   TPL +
Sbjct: 263 DFSFTSTATSLYGAKFSYCLVDHLSNKNVSNYLIFGS-------SRSTKTAFRRTTPLDL 322

Query: 301 NPLSPTFYYIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLK 360
             + P FY I V  I++    L I   VW  +    GGT++DSGT+LT LA+ AYK+V+ 
Sbjct: 323 TRI-PPFYAINVIGISLGYDMLDIPSQVW--DATSGGGTILDSGTSLTLLADAAYKQVVT 382

Query: 361 AM-RQRVKLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEE 420
            + R  V+L          + C + ++      LP++ F L  GA F P  ++Y +D   
Sbjct: 383 GLARYLVELKRVKPEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAP 442

Query: 421 GVMCLAIRAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGC 457
           GV CL   +  G    +VIGN+MQQ +L EFD  AS L F+   C
Sbjct: 443 GVKCLGFVSA-GTPATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

[Close]
BLAST of Csor.00g159670 vs. ExPASy Swiss-Prot
Match: Q9LHE3 (Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASPG2 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-50
Identity = 140/441 (31.75%), Postives = 220/441 (49.89%), Query Frame = 0 

Query: 36  LPLLHKNPFSSPS---------QALSSDTHRLSLLFSALRRRPNPT---------LKSPL 95
           L LLH++ F S +           +  DT R+S +   +  +  P+           S +
Sbjct: 61  LRLLHRDRFPSVTYRNHHHRLHARMRRDTDRVSAILRRISGKVIPSSDSRYEVNDFGSDI 120

Query: 96  ISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSS 155
           +SG   GSG+YFV + +G+PP+   +V D+GSD+VWV+C  C+ C +      F P  S 
Sbjct: 121 VSGMDQGSGEYFVRIGVGSPPRDQYMVIDSGSDMVWVQCQPCKLC-YKQSDPVFDPAKSG 180

Query: 156 SFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLSG 215
           S++   C    C  + ++  H          CR+   Y DGS     ++K T  L+TL+ 
Sbjct: 181 SYTGVSCGSSVCDRIENSGCH-------SGGCRYEVMYGDGS-----YTKGTLALETLTF 240

Query: 216 SETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMD 275
           ++T +++++ GCG R  G       F GA G++G+G G +SF  QL  + G  F YCL+ 
Sbjct: 241 AKTVVRNVAMGCGHRNRG------MFIGAAGLLGIGGGSMSFVGQLSGQTGGAFGYCLV- 300

Query: 276 YTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLPIN 335
            +     T  L+   G  +LPV      S+ PL+ NP +P+FYY+ +K + V GV++P+ 
Sbjct: 301 -SRGTDSTGSLVF--GREALPV----GASWVPLVRNPRAPSFYYVGLKGLGVGGVRIPLP 360

Query: 336 PIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMR-QRVKLPAAAELTPGFDLCVNV 395
             V+ + E G+GG V+D+GT +T L   AY       + Q   LP A+ ++  FD C ++
Sbjct: 361 DGVFDLTETGDGGVVMDTGTAVTRLPTAAYVAFRDGFKSQTANLPRASGVSI-FDTCYDL 420

Query: 396 SNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEE-GVMCLAIRAVEGGNGFSVIGNLMQ 455
           S       +P V F    G V   PARN+ +  ++ G  C A  A     G S+IGN+ Q
Sbjct: 421 SG-FVSVRVPTVSFYFTEGPVLTLPARNFLMPVDDSGTYCFAFAA--SPTGLSIIGNIQQ 470

Query: 456 QGFLLEFDKEASRLGFSRRGC 457
           +G  + FD     +GF    C
Sbjct: 481 EGIQVSFDGANGFVGFGPNVC 470

[Close]
BLAST of Csor.00g159670 vs. NCBI nr
Match: KAG6579697.1 (Aspartic proteinase NANA, chloroplast, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 922 bits (2382), Expect = 0.0
Identity = 459/459 (100.00%), Postives = 459/459 (100.00%), Query Frame = 0 

Query: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60
           MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL
Sbjct: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60

Query: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120
           LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180

Query: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240
           SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240

Query: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300
           TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY
Sbjct: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300

Query: 301 YIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360
           YIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL
Sbjct: 301 YIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360

Query: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRA 420
           PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRA
Sbjct: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRA 420

Query: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Csor.00g159670 vs. NCBI nr
Match: KAG7017139.1 (Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 914 bits (2362), Expect = 0.0
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0 

Query: 5   MLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 64
           MLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA
Sbjct: 1   MLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSA 60

Query: 65  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 124
           LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH
Sbjct: 61  LRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSH 120

Query: 125 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 184
           HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF
Sbjct: 121 HPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 180

Query: 185 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 244
           FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG
Sbjct: 181 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 240

Query: 245 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 304
           RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV
Sbjct: 241 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 300

Query: 305 KSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 364
           KSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA
Sbjct: 301 KSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 360

Query: 365 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGG 424
           ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGG
Sbjct: 361 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGG 420

Query: 425 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 421 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 455

[Close]
BLAST of Csor.00g159670 vs. NCBI nr
Match: XP_023549997.1 (aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 914 bits (2361), Expect = 0.0
Identity = 454/459 (98.91%), Postives = 458/459 (99.78%), Query Frame = 0 

Query: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60
           MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL
Sbjct: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60

Query: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120
           LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180

Query: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240
           SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240

Query: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300
           TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY
Sbjct: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300

Query: 301 YIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360
           YIAVKSITVDGVKLPINP VWAI+EQGNGGTVVDSGTTLTYLAEEAYKEVLKA+RQRVKL
Sbjct: 301 YIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKL 360

Query: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRA 420
           PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFL+TEEGVMCL+IRA
Sbjct: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLETEEGVMCLSIRA 420

Query: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Csor.00g159670 vs. NCBI nr
Match: XP_022928946.1 (aspartyl protease family protein 2-like [Cucurbita moschata])

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 451/459 (98.26%), Postives = 455/459 (99.13%), Query Frame = 0 

Query: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60
           MQTTMLSVSPFFH ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL
Sbjct: 1   MQTTMLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60

Query: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120
           LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180

Query: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240
           SSGFFSKETTTLKTL+GSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS
Sbjct: 181 SSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240

Query: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300
           TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLR LPVTNATKISYTPLLINPLSPTFY
Sbjct: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFY 300

Query: 301 YIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360
           YIAVKSITVDGVKLPINP +WAI+EQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL
Sbjct: 301 YIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360

Query: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRA 420
           PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVF PPARNYFL+TEEGVMCLAIRA
Sbjct: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRA 420

Query: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Csor.00g159670 vs. NCBI nr
Match: XP_022969957.1 (aspartyl protease family protein 2 [Cucurbita maxima])

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 449/459 (97.82%), Postives = 454/459 (98.91%), Query Frame = 0 

Query: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60
           MQTTMLSVS FFH ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL
Sbjct: 1   MQTTMLSVSTFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60

Query: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120
           LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180

Query: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240
           SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240

Query: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300
           TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY
Sbjct: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300

Query: 301 YIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360
           YIAVKSITVDGVKLPINP VWAI+EQGNGGTVVDSGTTLTYLAEEAYKEVLKA+RQRVKL
Sbjct: 301 YIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKL 360

Query: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRA 420
           PAAAELTPGFDLCVNVS ESQRPSLPRVRF++GNGAVFAPPARNYFL+T EGVMCLAIRA
Sbjct: 361 PAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRA 420

Query: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Csor.00g159670 vs. ExPASy TrEMBL
Match: A0A6J1ELQ4 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111435697 PE=3 SV=1)

HSP 1 Score: 908 bits (2346), Expect = 0.0
Identity = 451/459 (98.26%), Postives = 455/459 (99.13%), Query Frame = 0 

Query: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60
           MQTTMLSVSPFFH ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL
Sbjct: 1   MQTTMLSVSPFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60

Query: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120
           LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180

Query: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240
           SSGFFSKETTTLKTL+GSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS
Sbjct: 181 SSGFFSKETTTLKTLTGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240

Query: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300
           TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLR LPVTNATKISYTPLLINPLSPTFY
Sbjct: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRRLPVTNATKISYTPLLINPLSPTFY 300

Query: 301 YIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360
           YIAVKSITVDGVKLPINP +WAI+EQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL
Sbjct: 301 YIAVKSITVDGVKLPINPTLWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360

Query: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRA 420
           PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVF PPARNYFL+TEEGVMCLAIRA
Sbjct: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFPPPARNYFLETEEGVMCLAIRA 420

Query: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Csor.00g159670 vs. ExPASy TrEMBL
Match: A0A6J1HXS2 (aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 PE=3 SV=1)

HSP 1 Score: 902 bits (2330), Expect = 0.0
Identity = 449/459 (97.82%), Postives = 454/459 (98.91%), Query Frame = 0 

Query: 1   MQTTMLSVSPFFHFILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60
           MQTTMLSVS FFH ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL
Sbjct: 1   MQTTMLSVSTFFHLILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSL 60

Query: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120
           LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR
Sbjct: 61  LFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACR 120

Query: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180
           NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST
Sbjct: 121 NCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGST 180

Query: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240
           SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS
Sbjct: 181 SSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFS 240

Query: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300
           TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY
Sbjct: 241 TQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFY 300

Query: 301 YIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKL 360
           YIAVKSITVDGVKLPINP VWAI+EQGNGGTVVDSGTTLTYLAEEAYKEVLKA+RQRVKL
Sbjct: 301 YIAVKSITVDGVKLPINPTVWAIDEQGNGGTVVDSGTTLTYLAEEAYKEVLKAVRQRVKL 360

Query: 361 PAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRA 420
           PAAAELTPGFDLCVNVS ESQRPSLPRVRF++GNGAVFAPPARNYFL+T EGVMCLAIRA
Sbjct: 361 PAAAELTPGFDLCVNVSKESQRPSLPRVRFRVGNGAVFAPPARNYFLETVEGVMCLAIRA 420

Query: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP
Sbjct: 421 VEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459

[Close]
BLAST of Csor.00g159670 vs. ExPASy TrEMBL
Match: A0A0A0KNH6 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G174650 PE=3 SV=1)

HSP 1 Score: 790 bits (2040), Expect = 1.78e-286
Identity = 396/460 (86.09%), Postives = 419/460 (91.09%), Query Frame = 0 

Query: 6   LSVSPFFHFILLL-FSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRLS 65
           LS+SPFF  ILL  F L  L N      AA AD+LKLPLLHK PFSSPSQ+LSSDTHRLS
Sbjct: 4   LSISPFFLLILLFSFFLTHLPNPNATAVAAPADFLKLPLLHKPPFSSPSQSLSSDTHRLS 63

Query: 66  LLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSAC 125
           LLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSAC
Sbjct: 64  LLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSAC 123

Query: 126 RNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGS 185
           RNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAP HLCNHTRLHSPCRFLY+YADGS
Sbjct: 124 RNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPHHLCNHTRLHSPCRFLYSYADGS 183

Query: 186 TSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISF 245
            SSGFFSKETTTLK+LSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG ISF
Sbjct: 184 LSSGFFSKETTTLKSLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSISF 243

Query: 246 STQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTF 305
           S+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLP+TNATKISYTPL INPLSPTF
Sbjct: 244 SSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPLTNATKISYTPLQINPLSPTF 303

Query: 306 YYIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVK 365
           YYI + SIT+DGVKLPINP VW I+EQGNGGTVVDSGTTLTYL + AY+EVLK++R+RVK
Sbjct: 304 YYITIHSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRVK 363

Query: 366 LPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIR 425
           LP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVFAPP RNYFL+TEEGVMCLAIR
Sbjct: 364 LPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAIR 423

Query: 426 AVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           AVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 424 AVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]
BLAST of Csor.00g159670 vs. ExPASy TrEMBL
Match: A0A1S3CSZ8 (aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 SV=1)

HSP 1 Score: 790 bits (2040), Expect = 1.78e-286
Identity = 396/461 (85.90%), Postives = 417/461 (90.46%), Query Frame = 0 

Query: 5   MLSVSPFFHFI-LLLFSLADLLN-----AAAAADYLKLPLLHKNPFSSPSQALSSDTHRL 64
           MLS+SPFF  I L  F L  L N      AAAAD+LKLPLLHK PFSSPSQ+LSSDTHRL
Sbjct: 3   MLSISPFFLLIPLFFFFLTHLSNPNATAVAAAADFLKLPLLHKPPFSSPSQSLSSDTHRL 62

Query: 65  SLLFSALRRRPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSA 124
           SLLFS    RPNPTLKSPLISGASTGSGQYFVD+R+GTPPQSLLLVADTGSDLVWVKCSA
Sbjct: 63  SLLFS----RPNPTLKSPLISGASTGSGQYFVDIRLGTPPQSLLLVADTGSDLVWVKCSA 122

Query: 125 CRNCSHHPPSSAFLPRHSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADG 184
           CRNCSHHPPSSAF PRHSSSFSPFHCFDPHCRLLPHAPPH CNHT LHSPCRFLY+YADG
Sbjct: 123 CRNCSHHPPSSAFFPRHSSSFSPFHCFDPHCRLLPHAPPHHCNHTLLHSPCRFLYSYADG 182

Query: 185 STSSGFFSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPIS 244
           S SSGFFSKETTTLKTLSGSE  LK LSFGCGFRISGPSVSGAQFNGARGVMGLGRG IS
Sbjct: 183 SLSSGFFSKETTTLKTLSGSEIHLKGLSFGCGFRISGPSVSGAQFNGARGVMGLGRGSIS 242

Query: 245 FSTQLGRRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPT 304
           FS+QLGRRFGNKFSYCLMDYTLSPPPTS+LMIGGGL SLPV NATKISYTPL INPLSPT
Sbjct: 243 FSSQLGRRFGNKFSYCLMDYTLSPPPTSFLMIGGGLHSLPVNNATKISYTPLQINPLSPT 302

Query: 305 FYYIAVKSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV 364
           FYYI + SIT+DGVKLPINP VW I+EQGNGGTVVDSGTTLTYL + AY+EVLK++R+RV
Sbjct: 303 FYYITINSITIDGVKLPINPAVWEIDEQGNGGTVVDSGTTLTYLTKTAYEEVLKSVRRRV 362

Query: 365 KLPAAAELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAI 424
           KLP AAELTPGFDLCVN S ES+RPSLPR+RF+LG GAVFAPP RNYFL+TEEGVMCLAI
Sbjct: 363 KLPNAAELTPGFDLCVNASGESRRPSLPRLRFRLGGGAVFAPPPRNYFLETEEGVMCLAI 422

Query: 425 RAVEGGNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 459
           RAVE GNGFSVIGNLMQQGFLLEFDKE SRLGF+RRGCGLP
Sbjct: 423 RAVESGNGFSVIGNLMQQGFLLEFDKEESRLGFTRRGCGLP 459

[Close]
BLAST of Csor.00g159670 vs. ExPASy TrEMBL
Match: A0A6J1EPZ3 (aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC111436585 PE=3 SV=1)

HSP 1 Score: 748 bits (1930), Expect = 8.65e-270
Identity = 372/447 (83.22%), Postives = 400/447 (89.49%), Query Frame = 0 

Query: 15  ILLLFSLADLLNAAAAADYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPNPTLK 74
           +LLL SLADL NA  +  YLK PLLH NPFSSPSQALSSDTHRLSLLFSA   R +PTLK
Sbjct: 12  LLLLSSLADLSNAIPS-QYLKFPLLHTNPFSSPSQALSSDTHRLSLLFSA--HRHSPTLK 71

Query: 75  SPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPR 134
           SPLISGASTGSGQYFV+L +GTPPQSLLLV DTGSDLVWVKCS CRNCSHHPPSSAF PR
Sbjct: 72  SPLISGASTGSGQYFVNLHLGTPPQSLLLVVDTGSDLVWVKCSPCRNCSHHPPSSAFFPR 131

Query: 135 HSSSFSPFHCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKT 194
           HSSSFSPFHCFDPHCRLLPH P H CNHT LHSPC FLY+YAD S SSGFFSK+ TT  T
Sbjct: 132 HSSSFSPFHCFDPHCRLLPHPPSHRCNHTHLHSPCSFLYSYADSSLSSGFFSKDVTTFNT 191

Query: 195 LSGS--ETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFS 254
            SG+  +TRL DLSFGCGFRISGPSVSGA+F GARGVMGLGRGPISFS+QLG RFGN FS
Sbjct: 192 FSGTHTQTRLNDLSFGCGFRISGPSVSGARFTGARGVMGLGRGPISFSSQLGHRFGNTFS 251

Query: 255 YCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGV 314
           YCLMDYTLSPPPTSYLMIGGGLRSLPVTNA+KISYTPL INPLSPTFYYI VKSITVDGV
Sbjct: 252 YCLMDYTLSPPPTSYLMIGGGLRSLPVTNASKISYTPLQINPLSPTFYYIVVKSITVDGV 311

Query: 315 KLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDL 374
           KLPINP VWAI+EQGNGGTVVDSGTTLTYLAE AY+EVLKAMR+RVKLP A +L+PGFDL
Sbjct: 312 KLPINPKVWAIDEQGNGGTVVDSGTTLTYLAEAAYEEVLKAMRRRVKLPRALQLSPGFDL 371

Query: 375 CVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGGNGFSVIGN 434
           CVN S+ES+  SLP++RF++G G VFAPPARNYF++TEEGVMCLAIR V+ GNGFSVIGN
Sbjct: 372 CVNASSESRMRSLPQIRFRVGGGGVFAPPARNYFVETEEGVMCLAIRPVDSGNGFSVIGN 431

Query: 435 LMQQGFLLEFDKEASRLGFSRRGCGLP 459
           LMQQGFLLEFD+E SR+GFSRRGCGLP
Sbjct: 432 LMQQGFLLEFDREKSRMGFSRRGCGLP 455

[Close]
BLAST of Csor.00g159670 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 598.6 bits (1542), Expect = 4.3e-171
Identity = 300/456 (65.79%), Postives = 360/456 (78.95%), Query Frame = 0 

Query: 11  FFHFILLLFSLADLLNAAAAAD---YLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRR 70
           FF    L   L    N AA ++   YLKLPLL K+PF SP+QAL+ DT RL   F +LRR
Sbjct: 6   FFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLH--FLSLRR 65

Query: 71  RPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 130
           +P P +KSP++SGA++GSGQYFVDLRIG PPQSLLL+ADTGSDLVWVKCSACRNCSHH P
Sbjct: 66  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 125

Query: 131 SSAFLPRHSSSFSPFHCFDPHCRLLP---HAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 190
           ++ F PRHSS+FSP HC+DP CRL+P    AP  +CNHTR+HS C + Y YADGS +SG 
Sbjct: 126 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSLTSGL 185

Query: 191 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 250
           F++ETT+LKT SG E RLK ++FGCGFRISG SVSG  FNGA GVMGLGRGPISF++QLG
Sbjct: 186 FARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSFNGANGVMGLGRGPISFASQLG 245

Query: 251 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 310
           RRFGNKFSYCLMDYTLSPPPTSYL+IG G   +     +K+ +TPLL NPLSPTFYY+ +
Sbjct: 246 RRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDGI-----SKLFFTPLLTNPLSPTFYYVKL 305

Query: 311 KSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 370
           KS+ V+G KL I+P +W I++ GNGGTVVDSGTTL +LAE AY+ V+ A+R+RVKLP A 
Sbjct: 306 KSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 365

Query: 371 ELTPGFDLCVNVSNESQ-RPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEG 430
            LTPGFDLCVNVS  ++    LPR++F+   GAVF PP RNYF++TEE + CLAI++V+ 
Sbjct: 366 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 425

Query: 431 GNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 460
             GFSVIGNLMQQGFL EFD++ SRLGFSRRGC LP
Sbjct: 426 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 452

[Close]
BLAST of Csor.00g159670 vs. TAIR 10
Match: AT3G25700.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 407.1 bits (1045), Expect = 1.8e-113
Identity = 229/456 (50.22%), Postives = 274/456 (60.09%), Query Frame = 0 

Query: 11  FFHFILLLFSLADLLNAAAAAD---YLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRR 70
           FF    L   L    N AA ++   YLKLPLL K+PF SP+QAL+ DT RL   F +LRR
Sbjct: 6   FFLCSFLSLFLLPPSNIAAVSNHNKYLKLPLLRKSPFPSPTQALALDTRRLH--FLSLRR 65

Query: 71  RPNPTLKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPP 130
           +P P +KSP++SGA++GSGQYFVDLRIG PPQSLLL+ADTGSDLVWVKCSACRNCSHH P
Sbjct: 66  KPIPFVKSPVVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKCSACRNCSHHSP 125

Query: 131 SSAFLPRHSSSFSPFHCFDPHCRLLP---HAPPHLCNHTRLHSPCRFLYTYADGSTSSGF 190
           ++ F PRHSS+FSP HC+DP CRL+P    AP  +CNHTR+HS C + Y YADGS +SG 
Sbjct: 126 ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAP--ICNHTRIHSTCHYEYGYADGSLTSGL 185

Query: 191 FSKETTTLKTLSGSETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLG 250
           F++ETT+LKT SG E RLK ++FGCGFRISG SVS                         
Sbjct: 186 FARETTSLKTSSGKEARLKSVAFGCGFRISGQSVS------------------------- 245

Query: 251 RRFGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAV 310
                                                                       
Sbjct: 246 ------------------------------------------------------------ 305

Query: 311 KSITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAA 370
                                 GNGGTVVDSGTTL +LAE AY+ V+ A+R+RVKLP A 
Sbjct: 306 ----------------------GNGGTVVDSGTTLAFLAEPAYRSVIAAVRRRVKLPIAD 350

Query: 371 ELTPGFDLCVNVSNESQ-RPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEG 430
            LTPGFDLCVNVS  ++    LPR++F+   GAVF PP RNYF++TEE + CLAI++V+ 
Sbjct: 366 ALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIETEEQIQCLAIQSVDP 350

Query: 431 GNGFSVIGNLMQQGFLLEFDKEASRLGFSRRGCGLP 460
             GFSVIGNLMQQGFL EFD++ SRLGFSRRGC LP
Sbjct: 426 KVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCALP 350

[Close]
BLAST of Csor.00g159670 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 241.5 bits (615), Expect = 1.3e-63
Identity = 154/392 (39.29%), Postives = 214/392 (54.59%), Query Frame = 0 

Query: 73  LKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 132
           L + L SG + GSG+YF+D+ +GTPP+   L+ DTGSDL W++C  C +C  H     + 
Sbjct: 145 LIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQCLPCYDC-FHQNGMFYD 204

Query: 133 PRHSSSFSPFHCFDPHCRLLPHA-PPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTT 192
           P+ S+SF    C DP C L+    PP  C     +  C + Y Y D S ++G F+ ET T
Sbjct: 205 PKTSASFKNITCNDPRCSLISSPDPPVQCESD--NQSCPYFYWYGDRSNTTGDFAVETFT 264

Query: 193 --LKTLSG--SETRLKDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRF 252
             L T  G  SE ++ ++ FGCG    G       F+GA G++GLGRGP+SFS+QL   +
Sbjct: 265 VNLTTTEGGSSEYKVGNMMFGCGHWNRG------LFSGASGLLGLGRGPLSFSSQLQSLY 324

Query: 253 GNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSI 312
           G+ FSYCL+D   +   +S L+ G     L  TN    S+     N +  TFYYI +KSI
Sbjct: 325 GHSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHTNLNFTSFVNGKENSVE-TFYYIQIKSI 384

Query: 313 TVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVK--LPAAAE 372
            V G  L I    W I+  G+GGT++DSGTTL+Y AE AY+ +     +++K   P   +
Sbjct: 385 LVGGKALDIPEETWNISSDGDGGTIIDSGTTLSYFAEPAYEIIKNKFAEKMKENYPIFRD 444

Query: 373 LTPGFDLCVNVSN-ESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGG 432
             P  D C NVS  E     LP +     +G V+  PA N F+   E ++CLAI      
Sbjct: 445 F-PVLDPCFNVSGIEENNIHLPELGIAFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKS 504

Query: 433 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGC 457
             FS+IGN  QQ F + +D + SRLGF+   C
Sbjct: 505 T-FSIIGNYQQQNFHILYDTKRSRLGFTPTKC 524

[Close]
BLAST of Csor.00g159670 vs. TAIR 10
Match: AT1G01300.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 236.5 bits (602), Expect = 4.3e-62
Identity = 151/436 (34.63%), Postives = 224/436 (51.38%), Query Frame = 0 

Query: 32  DYLKLPLLHKNPFSSPSQALSSDTHRLSLLFSALRRRPNPTL---------KSPLISGAS 91
           D++     +K P    S  L  D+ R+  + +   + P   +          S ++SG S
Sbjct: 77  DHIDALSSNKTPDELFSSRLQRDSRRVKSIATLAAQIPGRNVTHAPRPGGFSSSVVSGLS 136

Query: 92  TGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFLPRHSSSFSPF 151
            GSG+YF  L +GTP + + +V DTGSD+VW++C+ CR C +      F PR S +++  
Sbjct: 137 QGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQCAPCRRC-YSQSDPIFDPRKSKTYATI 196

Query: 152 HCFDPHCRLLPHAPPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETTTLKTLSGSETRL 211
            C  PHCR L  A    CN  R    C +  +Y DGS + G FS ET T +       R+
Sbjct: 197 PCSSPHCRRLDSAG---CNTRR--KTCLYQVSYGDGSFTVGDFSTETLTFR-----RNRV 256

Query: 212 KDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRRFGNKFSYCLMDYTLSP 271
           K ++ GCG    G       F GA G++GLG+G +SF  Q G RF  KFSYCL+D + S 
Sbjct: 257 KGVALGCGHDNEG------LFVGAAGLLGLGKGKLSFPGQTGHRFNQKFSYCLVDRSASS 316

Query: 272 PPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKSITVDGVKLP-INPIVW 331
            P+S +     +  +         +TPLL NP   TFYY+ +  I+V G ++P +   ++
Sbjct: 317 KPSSVVFGNAAVSRI-------ARFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLF 376

Query: 332 AINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRVKLPAAAELTPGFDLCVNVSNESQ 391
            +++ GNGG ++DSGT++T L   AY  +  A R   K    A     FD C ++SN ++
Sbjct: 377 KLDQIGNGGVIIDSGTSVTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNE 436

Query: 392 RPSLPRVRFQLGNGAVFAPPARNYFLDTE-EGVMCLAIRAVEGGNGFSVIGNLMQQGFLL 451
              +P V      GA  + PA NY +  +  G  C A     G  G S+IGN+ QQGF +
Sbjct: 437 -VKVPTVVLHF-RGADVSLPATNYLIPVDTNGKFCFAFAGTMG--GLSIIGNIQQQGFRV 484

Query: 452 EFDKEASRLGFSRRGC 457
            +D  +SR+GF+  GC
Sbjct: 497 VYDLASSRVGFAPGGC 484

[Close]
BLAST of Csor.00g159670 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 226.9 bits (577), Expect = 3.4e-59
Identity = 146/392 (37.24%), Postives = 211/392 (53.83%), Query Frame = 0 

Query: 73  LKSPLISGASTGSGQYFVDLRIGTPPQSLLLVADTGSDLVWVKCSACRNCSHHPPSSAFL 132
           L + L SG + GSG+YF+D+ +G+PP+   L+ DTGSDL W++C  C +C     + AF 
Sbjct: 155 LVATLESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQCLPCYDCFQQ--NGAFY 214

Query: 133 -PRHSSSFSPFHCFDPHCRLLPHA-PPHLCNHTRLHSPCRFLYTYADGSTSSGFFSKETT 192
            P+ S+S+    C D  C L+    PP  C     +  C + Y Y D S ++G F+ ET 
Sbjct: 215 DPKASASYKNITCNDQRCNLVSSPDPPMPCKSD--NQSCPYYYWYGDSSNTTGDFAVETF 274

Query: 193 TLK-TLSGSETRL---KDLSFGCGFRISGPSVSGAQFNGARGVMGLGRGPISFSTQLGRR 252
           T+  T +G  + L   +++ FGCG    G       F+GA G++GLGRGP+SFS+QL   
Sbjct: 275 TVNLTTNGGSSELYNVENMMFGCGHWNRG------LFHGAAGLLGLGRGPLSFSSQLQSL 334

Query: 253 FGNKFSYCLMDYTLSPPPTSYLMIGGGLRSLPVTNATKISYTPLLINPLSPTFYYIAVKS 312
           +G+ FSYCL+D       +S L+ G     L   N    S+     N L  TFYY+ +KS
Sbjct: 335 YGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSHPNLNFTSFVAGKEN-LVDTFYYVQIKS 394

Query: 313 ITVDGVKLPINPIVWAINEQGNGGTVVDSGTTLTYLAEEAYKEVLKAMRQRV--KLPAAA 372
           I V G  L I    W I+  G GGT++DSGTTL+Y AE AY+ +   + ++   K P   
Sbjct: 395 ILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKNKIAEKAKGKYPVYR 454

Query: 373 ELTPGFDLCVNVSNESQRPSLPRVRFQLGNGAVFAPPARNYFLDTEEGVMCLAIRAVEGG 432
           +  P  D C NVS       LP +     +GAV+  P  N F+   E ++CLA+      
Sbjct: 455 DF-PILDPCFNVSG-IHNVQLPELGIAFADGAVWNFPTENSFIWLNEDLVCLAMLGTP-K 514

Query: 433 NGFSVIGNLMQQGFLLEFDKEASRLGFSRRGC 457
           + FS+IGN  QQ F + +D + SRLG++   C
Sbjct: 515 SAFSIIGNYQQQNFHILYDTKRSRLGYAPTKC 532

The following BLAST results are available for this feature:
BLAST of Csor.00g159670 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9LNJ36.0e-6134.63Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ... [more]
Q766C21.9e-5434.76Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S... [more]
Q766C32.3e-5234.40Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S... [more]
Q9LTW43.3e-5133.12Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE... [more]
Q9LHE32.1e-5031.75Protein ASPARTIC PROTEASE IN GUARD CELL 2 OS=Arabidopsis thaliana OX=3702 GN=ASP... [more]
BLAST of Csor.00g159670 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG6579697.10.0100.00Aspartic proteinase NANA, chloroplast, partial [Cucurbita argyrosperma subsp. so... [more]
KAG7017139.10.0100.00Aspartic proteinase nepenthesin-2, partial [Cucurbita argyrosperma subsp. argyro... [more]
XP_023549997.10.098.91aspartyl protease family protein 2 [Cucurbita pepo subsp. pepo][more]
XP_022928946.10.098.26aspartyl protease family protein 2-like [Cucurbita moschata][more]
XP_022969957.10.097.82aspartyl protease family protein 2 [Cucurbita maxima][more]
BLAST of Csor.00g159670 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1ELQ40.098.26aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
A0A6J1HXS20.097.82aspartyl protease family protein 2 OS=Cucurbita maxima OX=3661 GN=LOC111469001 P... [more]
A0A0A0KNH61.78e-28686.09Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G17465... [more]
A0A1S3CSZ81.78e-28685.90aspartyl protease family protein 2 OS=Cucumis melo OX=3656 GN=LOC103504612 PE=3 ... [more]
A0A6J1EPZ38.65e-27083.22aspartyl protease family protein 2-like OS=Cucurbita moschata OX=3662 GN=LOC1114... [more]
BLAST of Csor.00g159670 vs. TAIR 10
Match NameE-valueIdentityDescription
AT3G25700.14.3e-17165.79Eukaryotic aspartyl protease family protein [more]
AT3G25700.21.8e-11350.22Eukaryotic aspartyl protease family protein [more]
AT2G42980.11.3e-6339.29Eukaryotic aspartyl protease family protein [more]
AT1G01300.14.3e-6234.63Eukaryotic aspartyl protease family protein [more]
AT3G59080.13.4e-5937.24Eukaryotic aspartyl protease family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001461Aspartic peptidase A1 familyPRINTSPR00792PEPSINcoord: 428..443
score: 18.99
coord: 331..342
score: 41.24
coord: 94..114
score: 51.9
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 277..457
e-value: 8.0E-52
score: 177.6
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 66..271
e-value: 1.0E-46
score: 161.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 82..456
IPR032861Xylanase inhibitor, N-terminalPFAMPF14543TAXi_Ncoord: 88..272
e-value: 5.6E-49
score: 166.8
IPR032799Xylanase inhibitor, C-terminalPFAMPF14541TAXi_Ccoord: 299..452
e-value: 3.3E-37
score: 127.7
NoneNo IPR availablePANTHERPTHR47967:SF72BNAA02G27600D PROTEINcoord: 18..457
NoneNo IPR availablePANTHERPTHR47967OS07G0603500 PROTEIN-RELATEDcoord: 18..457
IPR033121Peptidase family A1 domainPROSITEPS51767PEPTIDASE_A1coord: 88..452
score: 41.398293
IPR034161Pepsin-like domain, plantCDDcd05476pepsin_A_like_plantcoord: 87..456
e-value: 5.68014E-89
score: 270.288

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g159670.m01Csor.00g159670.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004190 aspartic-type endopeptidase activity