Csor.00g073480 (gene) Silver-seed gourd (wild; sororia) v1

Overview


Name	Csor.00g073480
Type	gene
Organism	Cucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Description	aspartic proteinase NANA, chloroplast-like
Location	Csor_Chr02: 4175447 .. 4177119 (-)
RNA-Seq Expression	Csor.00g073480
Synteny	Csor.00g073480

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintronterminalstop_codon

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGCCGATTTCTCATCTTTTAATCCTTTTCTTCGTCTTCTTCTCTCCTCTCACCGTCGCAGTCGCCGATCAAAGCAACGCCAATAATCTCAAACAAGAAAGCGATGCCAATAATGAAGAACAAGAATTCGTGAGGCTAGATCTGATACACCGCCACCATCCGGAAGTGGTTAAAAGGATTGATGACGAAATTAAGGTGGATACTGTCGAGGATCGCATCAAGGATATTCGCTATCACGATCAAAACCGCCTCCGAGCCATCTCCGCCCACCTGAATTGGACCAAAGTTGTGGAGAATGCGGAGGAGAAGGAGAAGGAGGTTTCGGGTTCGAATCTGTCGCAGACGCCAATAGGATTGAAAATATACCCCGGCGCTGATTTCGGTAGCGGTGAATTTTTCGTGCAATTGAAAGTCGGAACGCCGCCGCAGACGTTCACACTAATTGCAGATACCGGAAGTGACCTATTGTGGACGAAATGCAGATTCCGGCGGTGCAGGGGAGATTGCAGCCACCTCTCTCCGATGCATAAGATGCGTAACAAAATGAGAGGGAGATTCAGATACGCGCTTTATGCGAATCAGTCGTCTTCTTTCTCCCCAATCCCTTGTTCCTCCAAGCAGTGCATCGATGATTTCCCTGATCTCGGCGGCCAACCCGATTGTCCAACCCCTAACACCCCCTGTTCCTATACCTACAGGTATTAATCATTATTATTATTATTATTTTTTTTAAATAAAATTTTTGGTGGGGACCATTATAACAATAAATGGATGTTGGTTGGTTTGTTTTGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATACAGCTACACAGGTGGGGAGCGTGCGAGTGGAATATTCGCAAACGAGACGGTAACGGTAAGACTAACAAACGGAAAAGAAAAGCAACTGAAGGACATTCTATTCGGCTGCACAGAAGAAGTTGAAGTCACCAACTTCATGAAGGGAGCCGATGGCCTCATTGGCTTAGGCTCTAGCATCTACTCCTTCGTCTACAAAGCCGCCGAAAACAACATCGGCGGCGGCTTCTCCTACTGCCTCGCCGATCACCACCGCAACATAACCGCCATTAGCTACTTCGTCTTCGGCACTCCTTCCCCCAAGACCTTCTCCGCCACCACCTCCTCTCCCATCGGCCCCCCCTCCACCACCAAACTCTTCACCGGCGGCCAATACAGCTGCTACTACGGTGTCCAACTGATCGGAATCTCCGTCGACGACCAGATCCTTAACATCCCCCGTCACGTCTGGAACATCAAGTCCGGGTGCGGCACCATCTTGGACACCGGCACCAGCCTGACGATGCTGACGGCACCGGCTCACGATGCGGTGATAGAAGCGATGGCTCCGAAGATCGCGAAATTCGGAAGAATGGAAAAGCAGAGGAACTTCGAACTTTGCTTCAATGACACTGAGTGGAATTTTGGTATGTCGCCGAAGCTTGGATTCCATTTCGAAGGTGGGGCGGTGTTCGAACCGCCGGATAGGAGCTACATCGTTTCGGCGTCATACCAATGTAGCTGTATTGCCATAACTTCTCTGCCATTTCCGTCAATCAATATCTTAGGGAATATTATTCAGCAAACTTACTTTTGGCAATTTGATTTACTTAAGGGATCCGTCACTTTTGCTCCCTCCGATTGCGCCTAG

mRNA sequence

ATGTCGCCGATTTCTCATCTTTTAATCCTTTTCTTCGTCTTCTTCTCTCCTCTCACCGTCGCAGTCGCCGATCAAAGCAACGCCAATAATCTCAAACAAGAAAGCGATGCCAATAATGAAGAACAAGAATTCGTGAGGCTAGATCTGATACACCGCCACCATCCGGAAGTGGTTAAAAGGATTGATGACGAAATTAAGGTGGATACTGTCGAGGATCGCATCAAGGATATTCGCTATCACGATCAAAACCGCCTCCGAGCCATCTCCGCCCACCTGAATTGGACCAAAGTTGTGGAGAATGCGGAGGAGAAGGAGAAGGAGGTTTCGGGTTCGAATCTGTCGCAGACGCCAATAGGATTGAAAATATACCCCGGCGCTGATTTCGGTAGCGGTGAATTTTTCGTGCAATTGAAAGTCGGAACGCCGCCGCAGACGTTCACACTAATTGCAGATACCGGAAGTGACCTATTGTGGACGAAATGCAGATTCCGGCGGTGCAGGGGAGATTGCAGCCACCTCTCTCCGATGCATAAGATGCGTAACAAAATGAGAGGGAGATTCAGATACGCGCTTTATGCGAATCAGTCGTCTTCTTTCTCCCCAATCCCTTGTTCCTCCAAGCAGTGCATCGATGATTTCCCTGATCTCGGCGGCCAACCCGATTGTCCAACCCCTAACACCCCCTGTTCCTATACCTACAGCTACACAGGTGGGGAGCGTGCGAGTGGAATATTCGCAAACGAGACGGTAACGGTAAGACTAACAAACGGAAAAGAAAAGCAACTGAAGGACATTCTATTCGGCTGCACAGAAGAAGTTGAAGTCACCAACTTCATGAAGGGAGCCGATGGCCTCATTGGCTTAGGCTCTAGCATCTACTCCTTCGTCTACAAAGCCGCCGAAAACAACATCGGCGGCGGCTTCTCCTACTGCCTCGCCGATCACCACCGCAACATAACCGCCATTAGCTACTTCGTCTTCGGCACTCCTTCCCCCAAGACCTTCTCCGCCACCACCTCCTCTCCCATCGGCCCCCCCTCCACCACCAAACTCTTCACCGGCGGCCAATACAGCTGCTACTACGGTGTCCAACTGATCGGAATCTCCGTCGACGACCAGATCCTTAACATCCCCCGTCACGTCTGGAACATCAAGTCCGGGTGCGGCACCATCTTGGACACCGGCACCAGCCTGACGATGCTGACGGCACCGGCTCACGATGCGGTGATAGAAGCGATGGCTCCGAAGATCGCGAAATTCGGAAGAATGGAAAAGCAGAGGAACTTCGAACTTTGCTTCAATGACACTGAGTGGAATTTTGGTATGTCGCCGAAGCTTGGATTCCATTTCGAAGGTGGGGCGGTGTTCGAACCGCCGGATAGGAGCTACATCGTTTCGGCGTCATACCAATGTAGCTGTATTGCCATAACTTCTCTGCCATTTCCGTCAATCAATATCTTAGGGAATATTATTCAGCAAACTTACTTTTGGCAATTTGATTTACTTAAGGGATCCGTCACTTTTGCTCCCTCCGATTGCGCCTAG

Coding sequence (CDS)

ATGTCGCCGATTTCTCATCTTTTAATCCTTTTCTTCGTCTTCTTCTCTCCTCTCACCGTCGCAGTCGCCGATCAAAGCAACGCCAATAATCTCAAACAAGAAAGCGATGCCAATAATGAAGAACAAGAATTCGTGAGGCTAGATCTGATACACCGCCACCATCCGGAAGTGGTTAAAAGGATTGATGACGAAATTAAGGTGGATACTGTCGAGGATCGCATCAAGGATATTCGCTATCACGATCAAAACCGCCTCCGAGCCATCTCCGCCCACCTGAATTGGACCAAAGTTGTGGAGAATGCGGAGGAGAAGGAGAAGGAGGTTTCGGGTTCGAATCTGTCGCAGACGCCAATAGGATTGAAAATATACCCCGGCGCTGATTTCGGTAGCGGTGAATTTTTCGTGCAATTGAAAGTCGGAACGCCGCCGCAGACGTTCACACTAATTGCAGATACCGGAAGTGACCTATTGTGGACGAAATGCAGATTCCGGCGGTGCAGGGGAGATTGCAGCCACCTCTCTCCGATGCATAAGATGCGTAACAAAATGAGAGGGAGATTCAGATACGCGCTTTATGCGAATCAGTCGTCTTCTTTCTCCCCAATCCCTTGTTCCTCCAAGCAGTGCATCGATGATTTCCCTGATCTCGGCGGCCAACCCGATTGTCCAACCCCTAACACCCCCTGTTCCTATACCTACAGCTACACAGGTGGGGAGCGTGCGAGTGGAATATTCGCAAACGAGACGGTAACGGTAAGACTAACAAACGGAAAAGAAAAGCAACTGAAGGACATTCTATTCGGCTGCACAGAAGAAGTTGAAGTCACCAACTTCATGAAGGGAGCCGATGGCCTCATTGGCTTAGGCTCTAGCATCTACTCCTTCGTCTACAAAGCCGCCGAAAACAACATCGGCGGCGGCTTCTCCTACTGCCTCGCCGATCACCACCGCAACATAACCGCCATTAGCTACTTCGTCTTCGGCACTCCTTCCCCCAAGACCTTCTCCGCCACCACCTCCTCTCCCATCGGCCCCCCCTCCACCACCAAACTCTTCACCGGCGGCCAATACAGCTGCTACTACGGTGTCCAACTGATCGGAATCTCCGTCGACGACCAGATCCTTAACATCCCCCGTCACGTCTGGAACATCAAGTCCGGGTGCGGCACCATCTTGGACACCGGCACCAGCCTGACGATGCTGACGGCACCGGCTCACGATGCGGTGATAGAAGCGATGGCTCCGAAGATCGCGAAATTCGGAAGAATGGAAAAGCAGAGGAACTTCGAACTTTGCTTCAATGACACTGAGTGGAATTTTGGTATGTCGCCGAAGCTTGGATTCCATTTCGAAGGTGGGGCGGTGTTCGAACCGCCGGATAGGAGCTACATCGTTTCGGCGTCATACCAATGTAGCTGTATTGCCATAACTTCTCTGCCATTTCCGTCAATCAATATCTTAGGGAATATTATTCAGCAAACTTACTTTTGGCAATTTGATTTACTTAAGGGATCCGTCACTTTTGCTCCCTCCGATTGCGCCTAG

Protein sequence

MSPISHLLILFFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKRIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQTPIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYTGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFVYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQYSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA

Homology

[Close]

BLAST of Csor.00g073480 vs. ExPASy Swiss-Prot
Match: Q9LTW4 (Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE=1 SV=1)

HSP 1 Score: 278.9 bits (712), Expect = 1.2e-73
Identity = 166/452 (36.73%), Postives = 248/452 (54.87%), Query Frame = 0


Query: 69  TVEDRIKD--IRYHDQNRLRAISAHLNWTKVVENAEEKEKE-VSGSNLSQTPIGLKIYPG 128
           TV D +KD  +R    +R   +   L+  + V  A++K    +S    S   + + +  G
Sbjct: 39  TVADSMKDTSVRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLISRKRNSTVGVKMDLGSG 98

Query: 129 ADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMRNKMRG 188
            D+G+ ++F +++VGTP + F ++ DTGS+L W  CR+ R RG  +              
Sbjct: 99  IDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVNCRY-RARGKDN-------------- 158

Query: 189 RFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYTGGERASGIF 248
             R    A++S SF  + C ++ C  D  +L     CPTP+TPCSY Y Y  G  A G+F
Sbjct: 159 --RRVFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVF 218

Query: 249 ANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFVYKAAENNIG 308
           A ET+TV LTNG+  +L   L GC+      +F +GADG++GL  S +SF    A +  G
Sbjct: 219 AKETITVGLTNGRMARLPGHLIGCSSSFTGQSF-QGADGVLGLAFSDFSFT-STATSLYG 278

Query: 309 GGFSYCLADHHRNITAISYFVFGTP-SPKT-FSATTSSPIGPPSTTKLFTGGQYSCYYGV 368
             FSYCL DH  N    +Y +FG+  S KT F  TT     P   T++        +Y +
Sbjct: 279 AKFSYCLVDHLSNKNVSNYLIFGSSRSTKTAFRRTT-----PLDLTRI------PPFYAI 338

Query: 369 QLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIAKFGRM 428
            +IGIS+   +L+IP  VW+  SG GTILD+GTSLT+L   A+  V+  +A  + +  R+
Sbjct: 339 NVIGISLGYDMLDIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRV 398

Query: 429 EKQR-NFELCFNDTE-WNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLPF 488
           + +    E CF+ T  +N    P+L FH +GGA FEP  +SY+V A+    C+   S   
Sbjct: 399 KPEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSAGT 458

Query: 489 PSINILGNIIQQTYFWQFDLLKGSVTFAPSDC 514
           P+ N++GNI+QQ Y W+FDL+  +++FAPS C
Sbjct: 459 PATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of Csor.00g073480 vs. ExPASy Swiss-Prot
Match: Q766C2 (Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 4.0e-37
Identity = 126/425 (29.65%), Postives = 193/425 (45.41%), Query Frame = 0


Query: 96  KVVENAEEKEKEVSGSNLSQTPIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSD 155
           + ++  E + + ++    S + I   +Y     G GE+ + + +GTP  +F+ I DTGSD
Sbjct: 63  RAIKRGERRMRSINAMLQSSSGIETPVYA----GDGEYLMNVAIGTPDSSFSAIMDTGSD 122

Query: 156 LLWTKCRFRRCRGDCSHLSPMHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPD 215
           L+WT+C    C    S  +P+   ++              SSSFS +PC S+ C D    
Sbjct: 123 LIWTQC--EPCTQCFSQPTPIFNPQD--------------SSSFSTLPCESQYCQD---- 182

Query: 216 LGGQPDCPTPNTPCSYTYSYTGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEV 275
               P     N  C YTY Y  G    G  A ET T   ++     + +I FGC E+ + 
Sbjct: 183 ---LPSETCNNNECQYTYGYGDGSTTQGYMATETFTFETSS-----VPNIAFGCGEDNQG 242

Query: 276 TNFMKGADGLIGLGSSIYSFVYKAAENNIG-GGFSYCLADHHRNITAISYFVFGTPSPKT 335
                GA GLIG+G    S       + +G G FSYC+              +G+ SP T
Sbjct: 243 FGQGNGA-GLIGMGWGPLSL-----PSQLGVGQFSYCMTS------------YGSSSPST 302

Query: 336 F---SATTSSPIGPPSTTKLFTGGQYSCYYGVQLIGISVDDQILNIPRHVWNIKSG--CG 395
               SA +  P G PSTT L        YY + L GI+V    L IP   + ++     G
Sbjct: 303 LALGSAASGVPEGSPSTT-LIHSSLNPTYYYITLQGITVGGDNLGIPSSTFQLQDDGTGG 362

Query: 396 TILDTGTSLTMLTAPAHDAVIEAMAPKIAKFGRMEKQRNFELCFND-TEWNFGMSPKLGF 455
            I+D+GT+LT L   A++AV +A   +I      E       CF   ++ +    P++  
Sbjct: 363 MIIDSGTTLTYLPQDAYNAVAQAFTDQINLPTVDESSSGLSTCFQQPSDGSTVQVPEISM 422

Query: 456 HFEGGAVFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTF 514
            F+GG V    +++ ++S +    C+A+ S     I+I GNI QQ     +DL   +V+F
Sbjct: 423 QFDGG-VLNLGEQNILISPAEGVICLAMGSSSQLGISIFGNIQQQETQVLYDLQNLAVSF 435

[Close]

BLAST of Csor.00g073480 vs. ExPASy Swiss-Prot
Match: Q6XBF8 (Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1)

HSP 1 Score: 151.4 bits (381), Expect = 2.8e-35
Identity = 112/385 (29.09%), Postives = 170/385 (44.16%), Query Frame = 0


Query: 130 SGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMRNKMRGRFRY 189
           SGE+ + + +GTPP     IADTGSDLLWT+C    C    + + P+   +         
Sbjct: 87  SGEYLMNVSIGTPPFPIMAIADTGSDLLWTQC--APCDDCYTQVDPLFDPKT-------- 146

Query: 190 ALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYTGGERASGIFANET 249
                 SS++  + CSS QC      L  Q  C T +  CSY+ SY       G  A +T
Sbjct: 147 ------SSTYKDVSCSSSQC----TALENQASCSTNDNTCSYSLSYGDNSYTKGNIAVDT 206

Query: 250 VTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFVYKAAENNIGGGFS 309
           +T+  ++ +  QLK+I+ GC        F K   G++GLG    S + K   ++I G FS
Sbjct: 207 LTLGSSDTRPMQLKNIIIGCGHN-NAGTFNKKGSGIVGLGGGPVSLI-KQLGDSIDGKFS 266

Query: 310 YCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQYSCYYGVQLIGIS 369
           YCL          S   FGT +  + S   S+P        L        +Y + L  IS
Sbjct: 267 YCLVPLTSKKDQTSKINFGTNAIVSGSGVVSTP--------LIAKASQETFYYLTLKSIS 326

Query: 370 VDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIAKFGRMEKQRNF 429
           V  + +           G   I+D+GT+LT+L    +  + +A+A  I    + + Q   
Sbjct: 327 VGSKQIQYSGSDSESSEG-NIIIDSGTTLTLLPTEFYSELEDAVASSIDAEKKQDPQSGL 386

Query: 430 ELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLPFPSINILGN 489
            LC++ T       P +  HF+G  V      ++ V  S    C A      PS +I GN
Sbjct: 387 SLCYSAT--GDLKVPVITMHFDGADVKLDSSNAF-VQVSEDLVCFAFRG--SPSFSIYGN 435

Query: 490 IIQQTYFWQFDLLKGSVTFAPSDCA 515
           + Q  +   +D +  +V+F P+DCA
Sbjct: 447 VAQMNFLVGYDTVSKTVSFKPTDCA 435

[Close]

BLAST of Csor.00g073480 vs. ExPASy Swiss-Prot
Match: Q9LNJ3 (Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 SV=1)

HSP 1 Score: 143.3 bits (360), Expect = 7.7e-33
Identity = 120/417 (28.78%), Postives = 175/417 (41.97%), Query Frame = 0


Query: 107 EVSGSNLSQTP----IGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCR 166
           ++ G N++  P        +  G   GSGE+F +L VGTP +   ++ DTGSD++W +C 
Sbjct: 112 QIPGRNVTHAPRPGGFSSSVVSGLSQGSGEYFTRLGVGTPARYVYMVLDTGSDIVWLQC- 171

Query: 167 FRRCRGDCSHLSPMHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQC--IDDFPDLGGQP 226
              CR   S   P+   R              +S +++ IPCSS  C  +D         
Sbjct: 172 -APCRRCYSQSDPIFDPR--------------KSKTYATIPCSSPHCRRLD-------SA 231

Query: 227 DCPTPNTPCSYTYSYTGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMK 286
            C T    C Y  SY  G    G F+ ET+T R       ++K +  GC  + E      
Sbjct: 232 GCNTRRKTCLYQVSYGDGSFTVGDFSTETLTFR-----RNRVKGVALGCGHDNE--GLFV 291

Query: 287 GADGLIGLGSSIYSFVYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTS 346
           GA GL+GLG    SF  +   +     FSYCL D   +           PS   F     
Sbjct: 292 GAAGLLGLGKGKLSFPGQTG-HRFNQKFSYCLVDRSAS---------SKPSSVVFGNAAV 351

Query: 347 SPIGPPSTTKLFTGGQYSCYYGVQLIGISV-DDQILNIPRHVWNIK--SGCGTILDTGTS 406
           S I     T L +  +   +Y V L+GISV   ++  +   ++ +      G I+D+GTS
Sbjct: 352 SRIA--RFTPLLSNPKLDTFYYVGLLGISVGGTRVPGVTASLFKLDQIGNGGVIIDSGTS 411

Query: 407 LTMLTAPAHDAVIEAMAPKIAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFE 466
           +T L  PA+ A+ +A         R      F+ CF+ +  N    P +  HF G  V  
Sbjct: 412 VTRLIRPAYIAMRDAFRVGAKTLKRAPDFSLFDTCFDLSNMNEVKVPTVVLHFRGADVSL 471

Query: 467 PPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 515
           P     I   +    C A        ++I+GNI QQ +   +DL    V FAP  CA
Sbjct: 472 PATNYLIPVDTNGKFCFAFAG-TMGGLSIIGNIQQQGFRVVYDLASSRVGFAPGGCA 485

[Close]

BLAST of Csor.00g073480 vs. ExPASy Swiss-Prot
Match: Q766C3 (Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.9e-31
Identity = 117/392 (29.85%), Postives = 167/392 (42.60%), Query Frame = 0


Query: 129 GSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMRNKMRGRFR 188
           G GE+ + L +GTP Q F+ I DTGSDL+WT+C  + C    +  +P+   +        
Sbjct: 91  GDGEYLMNLSIGTPAQPFSAIMDTGSDLIWTQC--QPCTQCFNQSTPIFNPQG------- 150

Query: 189 YALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYTGGERASGIFANE 248
                  SSSFS +PCSS+ C          P C   N  C YTY Y  G    G    E
Sbjct: 151 -------SSSFSTLPCSSQLC-----QALSSPTC--SNNFCQYTYGYGDGSETQGSMGTE 210

Query: 249 TVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFVYKAAENNIGGGF 308
           T+T    +     + +I FGC E  +      GA GL+G+G    S   +         F
Sbjct: 211 TLTFGSVS-----IPNITFGCGENNQGFGQGNGA-GLVGMGRGPLSLPSQLDVTK----F 270

Query: 309 SYCLADHHRNITAISYFVFGTPSPKTF---SATTSSPIGPPSTTKLFTGGQYSCYYGVQL 368
           SYC+               G+ +P      S   S   G P+TT L    Q   +Y + L
Sbjct: 271 SYCMTP------------IGSSTPSNLLLGSLANSVTAGSPNTT-LIQSSQIPTFYYITL 330

Query: 369 IGISVDDQILNIPRHVWNIKSGCGT---ILDTGTSLTMLTAPAHDAVIEAMAPKIAKFGR 428
            G+SV    L I    + + S  GT   I+D+GT+LT     A+ +V +    +I     
Sbjct: 331 NGLSVGSTRLPIDPSAFALNSNNGTGGIIIDSGTTLTYFVNNAYQSVRQEFISQINLPVV 390

Query: 429 MEKQRNFELCFNDTEWNFGMS-PKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLPF 488
                 F+LCF        +  P    HF+GG + E P  +Y +S S    C+A+ S   
Sbjct: 391 NGSSSGFDLCFQTPSDPSNLQIPTFVMHFDGGDL-ELPSENYFISPSNGLICLAMGS-SS 434

Query: 489 PSINILGNIIQQTYFWQFDLLKGSVTFAPSDC 514
             ++I GNI QQ     +D     V+FA + C
Sbjct: 451 QGMSIFGNIQQQNMLVVYDTGNSVVSFASAQC 434

[Close]

BLAST of Csor.00g073480 vs. NCBI nr
Match: KAG6605363.1 (Aspartic proteinase NANA, chloroplast, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1060 bits (2740), Expect = 0.0
Identity = 514/514 (100.00%), Postives = 514/514 (100.00%), Query Frame = 0


Query: 1   MSPISHLLILFFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKR 60
           MSPISHLLILFFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKR
Sbjct: 1   MSPISHLLILFFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKR 60

Query: 61  IDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQTPIGL 120
           IDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQTPIGL
Sbjct: 61  IDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQTPIGL 120

Query: 121 KIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMR 180
           KIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMR
Sbjct: 121 KIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMR 180

Query: 181 NKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYTGGER 240
           NKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYTGGER
Sbjct: 181 NKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYTGGER 240

Query: 241 ASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFVYKAA 300
           ASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFVYKAA
Sbjct: 241 ASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFVYKAA 300

Query: 301 ENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQYSCY 360
           ENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQYSCY
Sbjct: 301 ENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQYSCY 360

Query: 361 YGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIAKF 420
           YGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIAKF
Sbjct: 361 YGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIAKF 420

Query: 421 GRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLP 480
           GRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLP
Sbjct: 421 GRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLP 480

Query: 481 FPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514
           FPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA
Sbjct: 481 FPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514

[Close]

BLAST of Csor.00g073480 vs. NCBI nr
Match: XP_022947059.1 (aspartic proteinase NANA, chloroplast-like [Cucurbita moschata])

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 508/518 (98.07%), Postives = 511/518 (98.65%), Query Frame = 0


Query: 1   MSPISHLLILFFVF----FSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPE 60
           MSPISHLLIL FVF    FSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPE
Sbjct: 1   MSPISHLLILVFVFVFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPE 60

Query: 61  VVKRIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQT 120
           VVKRIDDEIKVD+VEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQT
Sbjct: 61  VVKRIDDEIKVDSVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQT 120

Query: 121 PIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPM 180
           PIGLK YPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPM
Sbjct: 121 PIGLKTYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPM 180

Query: 181 HKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYT 240
           HKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYT
Sbjct: 181 HKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYT 240

Query: 241 GGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFV 300
           GGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVT+FMKGADGLIGLGSSIYSFV
Sbjct: 241 GGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTDFMKGADGLIGLGSSIYSFV 300

Query: 301 YKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQ 360
           YKAAENNIGGGFSYCLADHHRN TAISYFVFGTPSPKTFSATTSSPIGPP+TTKLFTGGQ
Sbjct: 301 YKAAENNIGGGFSYCLADHHRNTTAISYFVFGTPSPKTFSATTSSPIGPPATTKLFTGGQ 360

Query: 361 YSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK 420
           YSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK
Sbjct: 361 YSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK 420

Query: 421 IAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAI 480
           IAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAI
Sbjct: 421 IAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAI 480

Query: 481 TSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514
           TSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA
Sbjct: 481 TSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 518

[Close]

BLAST of Csor.00g073480 vs. NCBI nr
Match: XP_023532727.1 (aspartic proteinase NANA, chloroplast-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1011 bits (2613), Expect = 0.0
Identity = 491/519 (94.61%), Postives = 507/519 (97.69%), Query Frame = 0


Query: 1   MSPISHLLILFFVF--FSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV 60
           MSPISH LILFFVF  FSP+TVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV
Sbjct: 1   MSPISHSLILFFVFVFFSPITVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV 60

Query: 61  KRIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNL---SQ 120
           KR+DDEIKVD+VEDRI+DIRYHDQNRLR+ISA LNWTKVVENAEEKEKEVSGSNL   SQ
Sbjct: 61  KRLDDEIKVDSVEDRIRDIRYHDQNRLRSISAKLNWTKVVENAEEKEKEVSGSNLPPQSQ 120

Query: 121 TPIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSP 180
           TPIGLK YPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCS+LSP
Sbjct: 121 TPIGLKTYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSNLSP 180

Query: 181 MHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSY 240
           MHKMRNKMRGRFRYALYANQSSSFSPIPCSS+QCIDDFPDLGGQPDCPTPNTPCSYTYSY
Sbjct: 181 MHKMRNKMRGRFRYALYANQSSSFSPIPCSSRQCIDDFPDLGGQPDCPTPNTPCSYTYSY 240

Query: 241 TGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSF 300
           TGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVE+TNFMKGADGLIGLGSSIYSF
Sbjct: 241 TGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVELTNFMKGADGLIGLGSSIYSF 300

Query: 301 VYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGG 360
           VYKAAENNIGGGFSYCLADH+RN TAISYFVFGTPSPKTFSATTSSPIGPP+TTKLFTGG
Sbjct: 301 VYKAAENNIGGGFSYCLADHNRNTTAISYFVFGTPSPKTFSATTSSPIGPPATTKLFTGG 360

Query: 361 QYSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAP 420
           QYSCYYGVQLIGISVDDQ+L IPRHVWNIKSGCGTILDTGTSLT+LTAPAHDAVIEAMAP
Sbjct: 361 QYSCYYGVQLIGISVDDQMLKIPRHVWNIKSGCGTILDTGTSLTLLTAPAHDAVIEAMAP 420

Query: 421 KIAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIA 480
           KIAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSY+VSA+ QCSC+A
Sbjct: 421 KIAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYVVSAATQCSCVA 480

Query: 481 ITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514
           I+SLPFPSINILGNIIQQTYFWQFDLLK SVTFAPSDCA
Sbjct: 481 ISSLPFPSINILGNIIQQTYFWQFDLLKESVTFAPSDCA 519

[Close]

BLAST of Csor.00g073480 vs. NCBI nr
Match: XP_023007158.1 (aspartic proteinase NANA, chloroplast-like [Cucurbita maxima])

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 459/525 (87.43%), Postives = 483/525 (92.00%), Query Frame = 0


Query: 1   MSPISHLLILFFV--FFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV 60
           MS ISHLLILFFV  FFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV
Sbjct: 1   MSSISHLLILFFVVFFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV 60

Query: 61  KRIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNL---SQ 120
           KR+ DEIKVD +EDRIKDIRYHDQ+RLRAISAHLNWTKVVENAEEK KE SGSN    SQ
Sbjct: 61  KRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEKVKEASGSNHPPHSQ 120

Query: 121 TPIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSP 180
           TPI LK YPGADFGS EFFVQLKVGTPPQ FT+IADTGSDLLWT+CR+RRCRGDCS+ SP
Sbjct: 121 TPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSP 180

Query: 181 MHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSY 240
           +HKMRNKMR RF YALYANQSSSFSPIPCSSKQCI DF +LGGQPDCPTPNTPCSYTYSY
Sbjct: 181 IHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSY 240

Query: 241 TGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSF 300
             G+RA GIFA ETVTVRLTNGKEKQLKDIL+GCTEE+  + F+ GADGLIGLGSSIYSF
Sbjct: 241 LSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSF 300

Query: 301 VYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGG 360
           VYKAAENN+GGGFSYCLADH RNITAISYFVFGTPSPKTFSA+TSSPIGPP+TTKLFTGG
Sbjct: 301 VYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTGG 360

Query: 361 QYSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAP 420
           +YSCYYGVQL GISVD QILNIP HVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAP
Sbjct: 361 RYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAP 420

Query: 421 KIAKFGRMEK------QRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASY 480
           KI KFGRMEK      ++NF+LCFNDTEWNFGM PKLGFHFE GAVFEPPDRSYIVSASY
Sbjct: 421 KIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASY 480

Query: 481 QCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514
           QCSCIAITSLPFPSINILGNIIQQT+ W++DLLKGSVTFAPSDCA
Sbjct: 481 QCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDCA 525

[Close]

BLAST of Csor.00g073480 vs. NCBI nr
Match: XP_022947824.1 (aspartic proteinase NANA, chloroplast-like [Cucurbita moschata])

HSP 1 Score: 931 bits (2405), Expect = 0.0
Identity = 453/524 (86.45%), Postives = 483/524 (92.18%), Query Frame = 0


Query: 1   MSPISHLLILFFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKR 60
           MSPISHLLILFFVFFSPLTVAVADQSNANN KQESDANNEE+EFVRLDLIHRHHPEVVKR
Sbjct: 1   MSPISHLLILFFVFFSPLTVAVADQSNANNPKQESDANNEEKEFVRLDLIHRHHPEVVKR 60

Query: 61  IDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEK-EVSGSNL---SQT 120
           + DEIKVD +EDRIKDIRYHDQ+RLRAIS H+NWTKVVENAEEKEK E S SNL   SQT
Sbjct: 61  LHDEIKVDKMEDRIKDIRYHDQSRLRAISVHMNWTKVVENAEEKEKKEASSSNLPPQSQT 120

Query: 121 PIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPM 180
           PI LK YPGADFGS EFFVQLK+GTPPQ FT+IADTGSDLLWT+CR+RRCRGDCS+ SP+
Sbjct: 121 PIALKTYPGADFGSSEFFVQLKLGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPI 180

Query: 181 HKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYT 240
           HKMRN+MR RF YALYANQSSSFSPIPCSSKQCI DF +LGGQPDCPTPN+PCSYTYSY 
Sbjct: 181 HKMRNRMRERFIYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNSPCSYTYSYL 240

Query: 241 GGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFV 300
            G+RA GIFA ETVTVRLTNGKEKQLKDIL+GCTEE+  + F+ GADGLIGLGSSIYSFV
Sbjct: 241 SGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEITDSQFLDGADGLIGLGSSIYSFV 300

Query: 301 YKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQ 360
           YKAAENN+GGGFSYCLADH RNITAISYFVFGTPSPKTF+A+TSSPIGPP+TT+L TGG+
Sbjct: 301 YKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFAASTSSPIGPPATTRLITGGR 360

Query: 361 YSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK 420
           YSCYYGVQL GISVD QILNIP HVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK
Sbjct: 361 YSCYYGVQLAGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK 420

Query: 421 IAKFGRMEK------QRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQ 480
           I KFGRME+      ++NF+LCFNDT+WNFGM PKLGFHFEGGAVFEPPDRSYIVSASYQ
Sbjct: 421 IEKFGRMERDVKGEREKNFKLCFNDTQWNFGMLPKLGFHFEGGAVFEPPDRSYIVSASYQ 480

Query: 481 CSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514
           CSCIAITSLPFPSINILGNIIQQTY WQFDLLKGSVTFAPSDCA
Sbjct: 481 CSCIAITSLPFPSINILGNIIQQTYLWQFDLLKGSVTFAPSDCA 524

[Close]

BLAST of Csor.00g073480 vs. ExPASy TrEMBL
Match: A0A6J1G5P4 (aspartic proteinase NANA, chloroplast-like OS=Cucurbita moschata OX=3662 GN=LOC111451044 PE=3 SV=1)

HSP 1 Score: 1043 bits (2696), Expect = 0.0
Identity = 508/518 (98.07%), Postives = 511/518 (98.65%), Query Frame = 0


Query: 1   MSPISHLLILFFVF----FSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPE 60
           MSPISHLLIL FVF    FSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPE
Sbjct: 1   MSPISHLLILVFVFVFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPE 60

Query: 61  VVKRIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQT 120
           VVKRIDDEIKVD+VEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQT
Sbjct: 61  VVKRIDDEIKVDSVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQT 120

Query: 121 PIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPM 180
           PIGLK YPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPM
Sbjct: 121 PIGLKTYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPM 180

Query: 181 HKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYT 240
           HKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYT
Sbjct: 181 HKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYT 240

Query: 241 GGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFV 300
           GGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVT+FMKGADGLIGLGSSIYSFV
Sbjct: 241 GGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTDFMKGADGLIGLGSSIYSFV 300

Query: 301 YKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQ 360
           YKAAENNIGGGFSYCLADHHRN TAISYFVFGTPSPKTFSATTSSPIGPP+TTKLFTGGQ
Sbjct: 301 YKAAENNIGGGFSYCLADHHRNTTAISYFVFGTPSPKTFSATTSSPIGPPATTKLFTGGQ 360

Query: 361 YSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK 420
           YSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK
Sbjct: 361 YSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK 420

Query: 421 IAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAI 480
           IAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAI
Sbjct: 421 IAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAI 480

Query: 481 TSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514
           TSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA
Sbjct: 481 TSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 518

[Close]

BLAST of Csor.00g073480 vs. ExPASy TrEMBL
Match: A0A6J1L6Y2 (aspartic proteinase NANA, chloroplast-like OS=Cucurbita maxima OX=3661 GN=LOC111499734 PE=3 SV=1)

HSP 1 Score: 934 bits (2415), Expect = 0.0
Identity = 459/525 (87.43%), Postives = 483/525 (92.00%), Query Frame = 0


Query: 1   MSPISHLLILFFV--FFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV 60
           MS ISHLLILFFV  FFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV
Sbjct: 1   MSSISHLLILFFVVFFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVV 60

Query: 61  KRIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNL---SQ 120
           KR+ DEIKVD +EDRIKDIRYHDQ+RLRAISAHLNWTKVVENAEEK KE SGSN    SQ
Sbjct: 61  KRLHDEIKVDKMEDRIKDIRYHDQSRLRAISAHLNWTKVVENAEEKVKEASGSNHPPHSQ 120

Query: 121 TPIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSP 180
           TPI LK YPGADFGS EFFVQLKVGTPPQ FT+IADTGSDLLWT+CR+RRCRGDCS+ SP
Sbjct: 121 TPIALKTYPGADFGSSEFFVQLKVGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSP 180

Query: 181 MHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSY 240
           +HKMRNKMR RF YALYANQSSSFSPIPCSSKQCI DF +LGGQPDCPTPNTPCSYTYSY
Sbjct: 181 IHKMRNKMRERFNYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNTPCSYTYSY 240

Query: 241 TGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSF 300
             G+RA GIFA ETVTVRLTNGKEKQLKDIL+GCTEE+  + F+ GADGLIGLGSSIYSF
Sbjct: 241 LSGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEMTDSQFLDGADGLIGLGSSIYSF 300

Query: 301 VYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGG 360
           VYKAAENN+GGGFSYCLADH RNITAISYFVFGTPSPKTFSA+TSSPIGPP+TTKLFTGG
Sbjct: 301 VYKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFSASTSSPIGPPATTKLFTGG 360

Query: 361 QYSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAP 420
           +YSCYYGVQL GISVD QILNIP HVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAP
Sbjct: 361 RYSCYYGVQLSGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAP 420

Query: 421 KIAKFGRMEK------QRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASY 480
           KI KFGRMEK      ++NF+LCFNDTEWNFGM PKLGFHFE GAVFEPPDRSYIVSASY
Sbjct: 421 KIEKFGRMEKDVKGEREKNFKLCFNDTEWNFGMLPKLGFHFEDGAVFEPPDRSYIVSASY 480

Query: 481 QCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514
           QCSCIAITSLPFPSINILGNIIQQT+ W++DLLKGSVTFAPSDCA
Sbjct: 481 QCSCIAITSLPFPSINILGNIIQQTFIWKYDLLKGSVTFAPSDCA 525

[Close]

BLAST of Csor.00g073480 vs. ExPASy TrEMBL
Match: A0A6J1G810 (aspartic proteinase NANA, chloroplast-like OS=Cucurbita moschata OX=3662 GN=LOC111451585 PE=3 SV=1)

HSP 1 Score: 931 bits (2405), Expect = 0.0
Identity = 453/524 (86.45%), Postives = 483/524 (92.18%), Query Frame = 0


Query: 1   MSPISHLLILFFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKR 60
           MSPISHLLILFFVFFSPLTVAVADQSNANN KQESDANNEE+EFVRLDLIHRHHPEVVKR
Sbjct: 1   MSPISHLLILFFVFFSPLTVAVADQSNANNPKQESDANNEEKEFVRLDLIHRHHPEVVKR 60

Query: 61  IDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEK-EVSGSNL---SQT 120
           + DEIKVD +EDRIKDIRYHDQ+RLRAIS H+NWTKVVENAEEKEK E S SNL   SQT
Sbjct: 61  LHDEIKVDKMEDRIKDIRYHDQSRLRAISVHMNWTKVVENAEEKEKKEASSSNLPPQSQT 120

Query: 121 PIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPM 180
           PI LK YPGADFGS EFFVQLK+GTPPQ FT+IADTGSDLLWT+CR+RRCRGDCS+ SP+
Sbjct: 121 PIALKTYPGADFGSSEFFVQLKLGTPPQKFTMIADTGSDLLWTRCRYRRCRGDCSNPSPI 180

Query: 181 HKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYT 240
           HKMRN+MR RF YALYANQSSSFSPIPCSSKQCI DF +LGGQPDCPTPN+PCSYTYSY 
Sbjct: 181 HKMRNRMRERFIYALYANQSSSFSPIPCSSKQCIQDFSELGGQPDCPTPNSPCSYTYSYL 240

Query: 241 GGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFV 300
            G+RA GIFA ETVTVRLTNGKEKQLKDIL+GCTEE+  + F+ GADGLIGLGSSIYSFV
Sbjct: 241 SGDRAMGIFATETVTVRLTNGKEKQLKDILYGCTEEITDSQFLDGADGLIGLGSSIYSFV 300

Query: 301 YKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQ 360
           YKAAENN+GGGFSYCLADH RNITAISYFVFGTPSPKTF+A+TSSPIGPP+TT+L TGG+
Sbjct: 301 YKAAENNVGGGFSYCLADHLRNITAISYFVFGTPSPKTFAASTSSPIGPPATTRLITGGR 360

Query: 361 YSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK 420
           YSCYYGVQL GISVD QILNIP HVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK
Sbjct: 361 YSCYYGVQLAGISVDGQILNIPPHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPK 420

Query: 421 IAKFGRMEK------QRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQ 480
           I KFGRME+      ++NF+LCFNDT+WNFGM PKLGFHFEGGAVFEPPDRSYIVSASYQ
Sbjct: 421 IEKFGRMERDVKGEREKNFKLCFNDTQWNFGMLPKLGFHFEGGAVFEPPDRSYIVSASYQ 480

Query: 481 CSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 514
           CSCIAITSLPFPSINILGNIIQQTY WQFDLLKGSVTFAPSDCA
Sbjct: 481 CSCIAITSLPFPSINILGNIIQQTYLWQFDLLKGSVTFAPSDCA 524

[Close]

BLAST of Csor.00g073480 vs. ExPASy TrEMBL
Match: A0A0A0KG92 (Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G134390 PE=3 SV=1)

HSP 1 Score: 523 bits (1346), Expect = 6.97e-179
Identity = 273/533 (51.22%), Postives = 357/533 (66.98%), Query Frame = 0


Query: 1   MSPISHLLILFFVFF--------SPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHR 60
           MSPIS+    FF F         S    A+ D+ N  N     + + +EQE ++ DL+HR
Sbjct: 8   MSPISNFCFFFFFFLLFFFLSFSSSFLFALGDEDNNFNNNNNINDDEDEQEIIKFDLLHR 67

Query: 61  HHPEVVKRIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEK-------E 120
           HHP+V ++I  ++K+  V +R+KDI  HD NR R+IS  +N  K VE+A  +       E
Sbjct: 68  HHPQVAEKIHGDMKIQDVSERMKDIHEHDHNRHRSISKSMN-QKQVEDARLRAEAEAATE 127

Query: 121 KEVSGSNL----SQTPIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKC 180
           +EV+ S +    + TPIG+++  GADFGS E+FV+LKVGTP QTF LIADTGSDL W KC
Sbjct: 128 EEVAKSAILPPATSTPIGMRMISGADFGSSEYFVELKVGTPAQTFMLIADTGSDLTWMKC 187

Query: 181 RFRRCRGDCSHLSPMHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPD 240
           R+RRC G+CS  +  HK +N+ + RFR+A  AN SSSF  + CSS  C +D  DL    +
Sbjct: 188 RYRRCFGNCSS-NVNHKSKNEKKQRFRHAFLANHSSSFKTVSCSSTMCTNDLADLFAVRE 247

Query: 241 CPTPNTPCSYTYSYTGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKG 300
           C  P +PC Y YSYTGG  A GIFA ET+TV LTNGKEKQL + + GCTE V+ + F  G
Sbjct: 248 CHNPTSPCVYDYSYTGGASAKGIFAWETLTVGLTNGKEKQLHNSIIGCTESVQGSVF-GG 307

Query: 301 ADGLIGLGSSIYSFVYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSS 360
           ADG++GLG+S YS  YKAAEN  GGGFSYCL DH  +  AISYFV G P+P T ++T+S+
Sbjct: 308 ADGVMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAISYFVLGIPTPSTSASTSSA 367

Query: 361 PIGPPST-TKLFTGGQYSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTM 420
            +    T TKL+ G  YS +YGV LIGIS +  +LNIP  VW+I SG GTI+D+GTSLT+
Sbjct: 368 KLPAKMTYTKLYVGDPYSSFYGVDLIGISANGIMLNIPSRVWDINSGGGTIIDSGTSLTI 427

Query: 421 LTAPAHDAVIEAMAPKIAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPD 480
           L APA D V+EA+ P++ KF ++E +  F+ CFN++++   M+PKL FHF  G VFEPP 
Sbjct: 428 LAAPAFDMVMEALTPRLKKFQQLEIEP-FDFCFNNSQYTHEMAPKLRFHFGDGTVFEPPT 487

Query: 481 RSYIVSASYQCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDC 513
           +SYIVS     SCI   S+PFP+ NI+GNI+QQ + WQFD  K  V FAPS+C
Sbjct: 488 KSYIVSVGKFISCIGFVSMPFPANNIIGNILQQNHLWQFDFQKRRVGFAPSEC 536

[Close]

BLAST of Csor.00g073480 vs. ExPASy TrEMBL
Match: A0A1S3C2F3 (aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1)

HSP 1 Score: 520 bits (1340), Expect = 4.45e-178
Identity = 270/530 (50.94%), Postives = 359/530 (67.74%), Query Frame = 0


Query: 1   MSPISH-----LLILFFVFFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHP 60
           MSPIS+     LL+ F  F S    A+ D++N  N   + D    EQ+ +R DL+HRHHP
Sbjct: 8   MSPISNFCFFFLLLFFLSFSSSFLFALGDEANNYNNNDDED----EQQTIRFDLLHRHHP 67

Query: 61  EVVKRIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEK-------EV 120
           +V ++++ ++K+  + +R+KDI  HD+NR R+IS  +N  K +E+A  + +       EV
Sbjct: 68  QVSEKLNGDMKIQDLHERMKDIHEHDRNRHRSISKSMN-QKQIEDARLRAEAEAATQVEV 127

Query: 121 SGSNL----SQTPIGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFR 180
           + S +    + TPIG+K+  GADFGS E+FVQLKVGTP QTF LIADTGSDL W KCR+R
Sbjct: 128 AKSAILPPATSTPIGMKMISGADFGSSEYFVQLKVGTPAQTFMLIADTGSDLTWLKCRYR 187

Query: 181 RCRGDCSHLSPMHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPT 240
           RC G+CS  +  HK +N+ + RFR+AL ANQSS+F  + CSS  C ++  +L    +C T
Sbjct: 188 RCFGNCSG-NVNHKSKNEKKQRFRHALLANQSSTFKTVSCSSTMCTNNLAELFAVAECDT 247

Query: 241 PNTPCSYTYSYTGGERASGIFANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADG 300
           P +PC Y YSY GG  A GIFA ET+TV LTNGKEKQL++ + GCTE V+  N   GADG
Sbjct: 248 PTSPCVYDYSYAGGASAKGIFAWETLTVGLTNGKEKQLRNSIIGCTEIVQ-GNVFDGADG 307

Query: 301 LIGLGSSIYSFVYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSS-PI 360
           ++GLG+S YS  YKAAEN  GGGFSYCL DH  +  A+SYFV G P+P T ++T+S+ P 
Sbjct: 308 VMGLGTSSYSLTYKAAENANGGGFSYCLVDHLTDQRAVSYFVLGVPTPSTSASTSSAKPP 367

Query: 361 GPPSTTKLFTGGQYSCYYGVQLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTA 420
              S TKL+ G  YS +YGV LIGIS D Q+LNIP  VW+   GCGTI+D+GTSLT+L  
Sbjct: 368 AKMSYTKLYVGDPYSSFYGVDLIGISADGQMLNIPPRVWDSYKGCGTIIDSGTSLTVLAT 427

Query: 421 PAHDAVIEAMAPKIAKFGRMEKQRNFELCFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSY 480
           PA D V+E +  ++ +F ++E +  F  CFN++++   M+PKL FHF  G VFEPP +SY
Sbjct: 428 PAFDVVMEVLTSRLKQFQQIEIEP-FNFCFNNSQYTHDMAPKLRFHFGDGTVFEPPTKSY 487

Query: 481 IVSASYQCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDC 513
           IVS     SCI I S+PFPS+NI+GNI+QQ + WQFD  K  V FA S+C
Sbjct: 488 IVSVGEFISCIGIVSMPFPSLNIIGNILQQNHLWQFDFQKRRVGFAASEC 529

[Close]

BLAST of Csor.00g073480 vs. TAIR 10
Match: AT3G12700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 278.9 bits (712), Expect = 8.4e-75
Identity = 166/452 (36.73%), Postives = 248/452 (54.87%), Query Frame = 0


Query: 69  TVEDRIKD--IRYHDQNRLRAISAHLNWTKVVENAEEKEKE-VSGSNLSQTPIGLKIYPG 128
           TV D +KD  +R    +R   +   L+  + V  A++K    +S    S   + + +  G
Sbjct: 39  TVADSMKDTSVRLKLAHRDTLLPKPLSRIEDVIGADQKRHSLISRKRNSTVGVKMDLGSG 98

Query: 129 ADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMRNKMRG 188
            D+G+ ++F +++VGTP + F ++ DTGS+L W  CR+ R RG  +              
Sbjct: 99  IDYGTAQYFTEIRVGTPAKKFRVVVDTGSELTWVNCRY-RARGKDN-------------- 158

Query: 189 RFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTPNTPCSYTYSYTGGERASGIF 248
             R    A++S SF  + C ++ C  D  +L     CPTP+TPCSY Y Y  G  A G+F
Sbjct: 159 --RRVFRADESKSFKTVGCLTQTCKVDLMNLFSLTTCPTPSTPCSYDYRYADGSAAQGVF 218

Query: 249 ANETVTVRLTNGKEKQLKDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFVYKAAENNIG 308
           A ET+TV LTNG+  +L   L GC+      +F +GADG++GL  S +SF    A +  G
Sbjct: 219 AKETITVGLTNGRMARLPGHLIGCSSSFTGQSF-QGADGVLGLAFSDFSFT-STATSLYG 278

Query: 309 GGFSYCLADHHRNITAISYFVFGTP-SPKT-FSATTSSPIGPPSTTKLFTGGQYSCYYGV 368
             FSYCL DH  N    +Y +FG+  S KT F  TT     P   T++        +Y +
Sbjct: 279 AKFSYCLVDHLSNKNVSNYLIFGSSRSTKTAFRRTT-----PLDLTRI------PPFYAI 338

Query: 369 QLIGISVDDQILNIPRHVWNIKSGCGTILDTGTSLTMLTAPAHDAVIEAMAPKIAKFGRM 428
            +IGIS+   +L+IP  VW+  SG GTILD+GTSLT+L   A+  V+  +A  + +  R+
Sbjct: 339 NVIGISLGYDMLDIPSQVWDATSGGGTILDSGTSLTLLADAAYKQVVTGLARYLVELKRV 398

Query: 429 EKQR-NFELCFNDTE-WNFGMSPKLGFHFEGGAVFEPPDRSYIVSASYQCSCIAITSLPF 488
           + +    E CF+ T  +N    P+L FH +GGA FEP  +SY+V A+    C+   S   
Sbjct: 399 KPEGVPIEYCFSFTSGFNVSKLPQLTFHLKGGARFEPHRKSYLVDAAPGVKCLGFVSAGT 458

Query: 489 PSINILGNIIQQTYFWQFDLLKGSVTFAPSDC 514
           P+ N++GNI+QQ Y W+FDL+  +++FAPS C
Sbjct: 459 PATNVIGNIMQQNYLWEFDLMASTLSFAPSAC 460

[Close]

BLAST of Csor.00g073480 vs. TAIR 10
Match: AT3G25700.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 208.4 bits (529), Expect = 1.4e-53
Identity = 136/408 (33.33%), Postives = 203/408 (49.75%), Query Frame = 0


Query: 122 IYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMRN 181
           +  GA  GSG++FV L++G PPQ+  LIADTGSDL+W KC    CR +CSH SP      
Sbjct: 73  VVSGAASGSGQYFVDLRIGQPPQSLLLIADTGSDLVWVKC--SACR-NCSHHSP------ 132

Query: 182 KMRGRFRYALYANQSSSFSPIPCSSKQC-IDDFPDLGGQPDCPTPNTPCSYTYSYTGGER 241
                     +   SS+FSP  C    C +   PD     +    ++ C Y Y Y  G  
Sbjct: 133 ------ATVFFPRHSSTFSPAHCYDPVCRLVPKPDRAPICNHTRIHSTCHYEYGYADGSL 192

Query: 242 ASGIFANETVTVRLTNGKEKQLKDILFGC-----TEEVEVTNFMKGADGLIGLGSSIYSF 301
            SG+FA ET +++ ++GKE +LK + FGC      + V  T+F  GA+G++GLG    SF
Sbjct: 193 TSGLFARETTSLKTSSGKEARLKSVAFGCGFRISGQSVSGTSF-NGANGVMGLGRGPISF 252

Query: 302 VYKAAENNIGGGFSYCLADHHRNITAISYFVFGTP----SPKTFSATTSSPIGPPSTTKL 361
             +      G  FSYCL D+  +    SY + G      S   F+   ++P+ P      
Sbjct: 253 ASQLG-RRFGNKFSYCLMDYTLSPPPTSYLIIGNGGDGISKLFFTPLLTNPLSP------ 312

Query: 362 FTGGQYSCYYGVQLIGISVDDQILNIPRHVWNI--KSGCGTILDTGTSLTMLTAPAHDAV 421
                   +Y V+L  + V+   L I   +W I      GT++D+GT+L  L  PA+ +V
Sbjct: 313 -------TFYYVKLKSVFVNGAKLRIDPSIWEIDDSGNGGTVVDSGTTLAFLAEPAYRSV 372

Query: 422 IEAMAPKIAKFGRMEKQRNFELCFN--DTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSA 481
           I A+  ++           F+LC N         + P+L F F GGAVF PP R+Y +  
Sbjct: 373 IAAVRRRVKLPIADALTPGFDLCVNVSGVTKPEKILPRLKFEFSGGAVFVPPPRNYFIET 432

Query: 482 SYQCSCIAITSL-PFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 515
             Q  C+AI S+ P    +++GN++QQ + ++FD  +  + F+   CA
Sbjct: 433 EEQIQCLAIQSVDPKVGFSVIGNLMQQGFLFEFDRDRSRLGFSRRGCA 450

[Close]

BLAST of Csor.00g073480 vs. TAIR 10
Match: AT2G42980.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 177.6 bits (449), Expect = 2.6e-44
Identity = 151/486 (31.07%), Postives = 219/486 (45.06%), Query Frame = 0


Query: 53  HHPEVVK---RIDDEIKVDTVEDRIKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVS 112
           H  E VK   RI  E K  T    + D++  D  R++ + A  N +K  +N + ++K  S
Sbjct: 73  HTRESVKPQSRIKQETKRTT--HSVVDLQIQDLTRIKTLHARFNKSKKQKNEKVRKKITS 132

Query: 113 GSNLSQTP------IGLKIYPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRF 172
             +L   P      +   +  G   GSGE+F+ + VGTPP+ F+LI DTGSDL W +C  
Sbjct: 133 DISLVGAPEVSPGKLIATLESGMTLGSGEYFMDVLVGTPPKHFSLILDTGSDLNWLQC-- 192

Query: 173 RRCRGDCSHLSPMHKMRNKMRGRFRYALYANQSSSFSPIPCSSKQC-IDDFPDLGGQPDC 232
             C  DC H + M                   S+SF  I C+  +C +   PD   Q  C
Sbjct: 193 LPCY-DCFHQNGMF-------------YDPKTSASFKNITCNDPRCSLISSPDPPVQ--C 252

Query: 233 PTPNTPCSYTYSYTGGERASGIFANETVTVRLT----NGKEKQLKDILFGCTEEVEVTNF 292
            + N  C Y Y Y      +G FA ET TV LT       E ++ +++FGC         
Sbjct: 253 ESDNQSCPYFYWYGDRSNTTGDFAVETFTVNLTTTEGGSSEYKVGNMMFGCGHWNR--GL 312

Query: 293 MKGADGLIGLGSSIYSFVYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSP---KTF 352
             GA GL+GLG    SF     ++  G  FSYCL D + N    S  +FG        T 
Sbjct: 313 FSGASGLLGLGRGPLSF-SSQLQSLYGHSFSYCLVDRNSNTNVSSKLIFGEDKDLLNHTN 372

Query: 353 SATTSSPIGPPSTTKLFTGGQYSCYYGVQLIGISVDDQILNIPRHVWNIKS--GCGTILD 412
              TS   G  ++ + F        Y +Q+  I V  + L+IP   WNI S    GTI+D
Sbjct: 373 LNFTSFVNGKENSVETF--------YYIQIKSILVGGKALDIPEETWNISSDGDGGTIID 432

Query: 413 TGTSLTMLTAPAHDAVIEAMAPKIAKFGRMEKQRNFEL---CFN--DTEWNFGMSPKLGF 472
           +GT+L+    PA++ +    A K+ +       R+F +   CFN    E N    P+LG 
Sbjct: 433 SGTTLSYFAEPAYEIIKNKFAEKMKE--NYPIFRDFPVLDPCFNVSGIEENNIHLPELGI 492

Query: 473 HFEGGAVFEPPDRSYIVSASYQCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTF 515
            F  G V+  P  +  +  S    C+AI   P  + +I+GN  QQ +   +D  +  + F
Sbjct: 493 AFVDGTVWNFPAENSFIWLSEDLVCLAILGTPKSTFSIIGNYQQQNFHILYDTKRSRLGF 525

[Close]

BLAST of Csor.00g073480 vs. TAIR 10
Match: AT3G59080.1 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 171.8 bits (434), Expect = 1.4e-42
Identity = 147/528 (27.84%), Postives = 232/528 (43.94%), Query Frame = 0


Query: 14  FFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKRIDDEIKVDTVEDR 73
           F +P+    A  S +N+    S      +E    +   + H   +KR +         + 
Sbjct: 43  FPNPMRFGSASSSTSNDCGFSSPEKEPTKERTGENKTVKFH---LKRRETTTTEKATTNS 102

Query: 74  IKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQTPIGLKI----------- 133
           + +++  D  R++ +   +          +K+K+     ++ TP+   +           
Sbjct: 103 VLELQIRDLTRIQTLHKRVLEKNNQNTVSQKQKKNDKEVVTTTPVASSVEEQAGQLVATL 162

Query: 134 YPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMRNK 193
             G   GSGE+F+ + VG+PP+ F+LI DTGSDL W +C    C  DC           +
Sbjct: 163 ESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQC--LPCY-DCF----------Q 222

Query: 194 MRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDCPTP----NTPCSYTYSYTGG 253
             G F        S+S+  I C+ ++C     +L   PD P P    N  C Y Y Y   
Sbjct: 223 QNGAF---YDPKASASYKNITCNDQRC-----NLVSSPDPPMPCKSDNQSCPYYYWYGDS 282

Query: 254 ERASGIFANETVTVRL-TNGKEKQL---KDILFGCTEEVEVTNFMKGADGLIGLGSSIYS 313
              +G FA ET TV L TNG   +L   ++++FGC           GA GL+GLG    S
Sbjct: 283 SNTTGDFAVETFTVNLTTNGGSSELYNVENMMFGCGHWNR--GLFHGAAGLLGLGRGPLS 342

Query: 314 FVYKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTG 373
           F     ++  G  FSYCL D + +    S  +FG                P      F  
Sbjct: 343 F-SSQLQSLYGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSH--------PNLNFTSFVA 402

Query: 374 GQ---YSCYYGVQLIGISVDDQILNIPRHVWNIKS--GCGTILDTGTSLTMLTAPAHDAV 433
           G+      +Y VQ+  I V  ++LNIP   WNI S    GTI+D+GT+L+    PA++ +
Sbjct: 403 GKENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFI 462

Query: 434 IEAMAPKIAKFGRMEKQRNFEL---CFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVS 493
              +A K AK G+    R+F +   CFN +  +    P+LG  F  GAV+  P  +  + 
Sbjct: 463 KNKIAEK-AK-GKYPVYRDFPILDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIW 522

Query: 494 ASYQCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 515
            +    C+A+   P  + +I+GN  QQ +   +D  +  + +AP+ CA
Sbjct: 523 LNEDLVCLAMLGTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPTKCA 533

[Close]

BLAST of Csor.00g073480 vs. TAIR 10
Match: AT3G59080.2 (Eukaryotic aspartyl protease family protein )

HSP 1 Score: 153.3 bits (386), Expect = 5.3e-37
Identity = 136/526 (25.86%), Postives = 215/526 (40.87%), Query Frame = 0


Query: 14  FFSPLTVAVADQSNANNLKQESDANNEEQEFVRLDLIHRHHPEVVKRIDDEIKVDTVEDR 73
           F +P+    A  S +N+    S      +E    +   + H   +KR +         + 
Sbjct: 43  FPNPMRFGSASSSTSNDCGFSSPEKEPTKERTGENKTVKFH---LKRRETTTTEKATTNS 102

Query: 74  IKDIRYHDQNRLRAISAHLNWTKVVENAEEKEKEVSGSNLSQTPIGLKI----------- 133
           + +++  D  R++ +   +          +K+K+     ++ TP+   +           
Sbjct: 103 VLELQIRDLTRIQTLHKRVLEKNNQNTVSQKQKKNDKEVVTTTPVASSVEEQAGQLVATL 162

Query: 134 YPGADFGSGEFFVQLKVGTPPQTFTLIADTGSDLLWTKCRFRRCRGDCSHLSPMHKMRNK 193
             G   GSGE+F+ + VG+PP+ F+LI DTGSDL W +C                     
Sbjct: 163 ESGMTLGSGEYFMDVLVGSPPKHFSLILDTGSDLNWIQC--------------------- 222

Query: 194 MRGRFRYALYANQSSSFSPIPCSSKQCIDDFPDLGGQPDC--PTPNTPCSYTYSYTGGER 253
                              +PC                DC     N  C Y Y Y     
Sbjct: 223 -------------------LPCY---------------DCFQQNDNQSCPYYYWYGDSSN 282

Query: 254 ASGIFANETVTVRL-TNGKEKQL---KDILFGCTEEVEVTNFMKGADGLIGLGSSIYSFV 313
            +G FA ET TV L TNG   +L   ++++FGC           GA GL+GLG    SF 
Sbjct: 283 TTGDFAVETFTVNLTTNGGSSELYNVENMMFGCGHWNR--GLFHGAAGLLGLGRGPLSF- 342

Query: 314 YKAAENNIGGGFSYCLADHHRNITAISYFVFGTPSPKTFSATTSSPIGPPSTTKLFTGGQ 373
               ++  G  FSYCL D + +    S  +FG                P      F  G+
Sbjct: 343 SSQLQSLYGHSFSYCLVDRNSDTNVSSKLIFGEDKDLLSH--------PNLNFTSFVAGK 402

Query: 374 ---YSCYYGVQLIGISVDDQILNIPRHVWNIKS--GCGTILDTGTSLTMLTAPAHDAVIE 433
                 +Y VQ+  I V  ++LNIP   WNI S    GTI+D+GT+L+    PA++ +  
Sbjct: 403 ENLVDTFYYVQIKSILVAGEVLNIPEETWNISSDGAGGTIIDSGTTLSYFAEPAYEFIKN 462

Query: 434 AMAPKIAKFGRMEKQRNFEL---CFNDTEWNFGMSPKLGFHFEGGAVFEPPDRSYIVSAS 493
            +A K AK G+    R+F +   CFN +  +    P+LG  F  GAV+  P  +  +  +
Sbjct: 463 KIAEK-AK-GKYPVYRDFPILDPCFNVSGIHNVQLPELGIAFADGAVWNFPTENSFIWLN 497

Query: 494 YQCSCIAITSLPFPSINILGNIIQQTYFWQFDLLKGSVTFAPSDCA 515
               C+A+   P  + +I+GN  QQ +   +D  +  + +AP+ CA
Sbjct: 523 EDLVCLAMLGTPKSAFSIIGNYQQQNFHILYDTKRSRLGYAPTKCA 497

The following BLAST results are available for this feature:

BLAST of Csor.00g073480 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LTW4	1.2e-73	36.73	Aspartic proteinase NANA, chloroplast OS=Arabidopsis thaliana OX=3702 GN=NANA PE...	[more]
Q766C2	4.0e-37	29.65	Aspartic proteinase nepenthesin-2 OS=Nepenthes gracilis OX=150966 GN=nep2 PE=1 S...	[more]
Q6XBF8	2.8e-35	29.09	Aspartic proteinase CDR1 OS=Arabidopsis thaliana OX=3702 GN=CDR1 PE=1 SV=1	[more]
Q9LNJ3	7.7e-33	28.78	Aspartyl protease family protein 2 OS=Arabidopsis thaliana OX=3702 GN=APF2 PE=2 ...	[more]
Q766C3	1.9e-31	29.85	Aspartic proteinase nepenthesin-1 OS=Nepenthes gracilis OX=150966 GN=nep1 PE=1 S...	[more]

BLAST of Csor.00g073480 vs. NCBI nr


Match Name	Identity	Description
KAG6605363.1	100.00	Aspartic proteinase NANA, chloroplast, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022947059.1	98.07	aspartic proteinase NANA, chloroplast-like [Cucurbita moschata]	[more]
XP_023532727.1	94.61	aspartic proteinase NANA, chloroplast-like [Cucurbita pepo subsp. pepo]	[more]
XP_023007158.1	87.43	aspartic proteinase NANA, chloroplast-like [Cucurbita maxima]	[more]
XP_022947824.1	86.45	aspartic proteinase NANA, chloroplast-like [Cucurbita moschata]	[more]

BLAST of Csor.00g073480 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G5P4	0.0	98.07	aspartic proteinase NANA, chloroplast-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1L6Y2	0.0	87.43	aspartic proteinase NANA, chloroplast-like OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1G810	0.0	86.45	aspartic proteinase NANA, chloroplast-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A0A0KG92	6.97e-179	51.22	Peptidase A1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G13439...	[more]
A0A1S3C2F3	4.45e-178	50.94	aspartic proteinase CDR1 OS=Cucumis melo OX=3656 GN=LOC103496268 PE=3 SV=1	[more]

BLAST of Csor.00g073480 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G12700.1	8.4e-75	36.73	Eukaryotic aspartyl protease family protein	[more]
AT3G25700.1	1.4e-53	33.33	Eukaryotic aspartyl protease family protein	[more]
AT2G42980.1	2.6e-44	31.07	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.1	1.4e-42	27.84	Eukaryotic aspartyl protease family protein	[more]
AT3G59080.2	5.3e-37	25.86	Eukaryotic aspartyl protease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 23..43
None	No IPR available	PANTHER	PTHR47967:SF69	ASPARTIC PROTEINASE NANA, CHLOROPLAST	coord: 33..513
None	No IPR available	PANTHER	PTHR47967	OS07G0603500 PROTEIN-RELATED	coord: 33..513
IPR001461	Aspartic peptidase A1 family	PRINTS	PR00792	PEPSIN	coord: 139..159 score: 54.9 coord: 485..500 score: 32.64 coord: 390..401 score: 51.54
IPR032799	Xylanase inhibitor, C-terminal	PFAM	PF14541	TAXi_C	coord: 361..509 e-value: 2.1E-25 score: 89.4
IPR032861	Xylanase inhibitor, N-terminal	PFAM	PF14543	TAXi_N	coord: 133..328 e-value: 4.7E-44 score: 150.7
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 115..328 e-value: 2.3E-41 score: 143.9
IPR021109	Aspartic peptidase domain superfamily	GENE3D	2.40.70.10	Acid Proteases	coord: 334..514 e-value: 3.1E-33 score: 116.8
IPR021109	Aspartic peptidase domain superfamily	SUPERFAMILY	50630	Acid proteases	coord: 125..513
IPR033121	Peptidase family A1 domain	PROSITE	PS51767	PEPTIDASE_A1	coord: 133..509 score: 33.043522
IPR034161	Pepsin-like domain, plant	CDD	cd05476	pepsin_A_like_plant	coord: 132..513 e-value: 1.2521E-65 score: 211.738

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Csor.00g073480.m01	Csor.00g073480.m01	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
cellular_component	GO:0110165	cellular anatomical entity
molecular_function	GO:0004190	aspartic-type endopeptidase activity