Csor.00g027340 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g027340
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioncucumisin-like
LocationCsor_Chr04: 5961552 .. 5964759 (+)
RNA-Seq ExpressionCsor.00g027340
SyntenyCsor.00g027340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: CDSstart_codoninitialpolypeptideintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTTCTTCTCTGCTCTTAAATCTCCTCTTCCTTGCCCTCTTTTGCAGTGTGCTGGCATCCACCTCCCATTCCCACGATGATGATAGGAAGATATATATTGTGTACATGGGGAGCAAGCTACAGGACCCTTCTTCCGCTCATTTACATCACCGAGCAATGTTGGAACAAGTGGTTGGCAGGTTTATATATACACACTCTTCATCAACAATTATTTGAACAAATTATTTGAACAATTATTTGAACAAATTATTTGAACAATTATTTGAAATTTTTTTGTATGCTGTTTCAGCACTTTTGATCCACAATCTGTGTTATACACCTACAATAGAAGTTTCAACGGATTCGTGGTGAGACTCACTGAAGAAGAAGCTCAAGAGATTGCTAGTAAGCATATCTTTTATTTTTATTTAATGAGTTATTTTTGAAATTGAGTATAAAATTTGGAATGTTTTTCAGGCAAGGAGGGTGTGGTCTCTGTGTTCCTCAATCAAAACAACCATCTCCACACGACAAGATCATGGGATTTTATGGGGTTTCCACAAAATGTTGGTCGCGTACAACAACTGGAAAGCAACATAGTTGTTGGAGTTTTGGACAGCGGAATCTGGCCAGAGTCTCCCAGTTTCAACGATGATGGTTTTGGTTCTCCACCATCCAAGTGGAGGGGCAGATGCCAAGACTCCAATTTCAGTTGCAATAGGTAAACCATTTTGAATGAAAGTCTCACGTCCACTAATTTAGGAAATGATCATCAATTTATAACCCAAAACAAACAAAACTACGAGAACTCCGAATTAACAAACCACCACAGCTTCTATGATTCAAATTAACTTACTTTGGCATTACTTCTTAAAACCTACCCTCCTTTCCTTTGTCCCTTGCTAATTACTAATTAAACATGCAAATCCTTGGCACTTTGTTAGAAAACTTATCGGAGGTCGAGCATATCACATCGGCAGACCCATTCCCCCAGGCGATGTTGGTAGTCCAAGAGATACCGACGGCCATGGCACGCACACTGCATCCACGGTGGCTGGTGGTCTCGTGAGCCAGGCAAGTCTGTATGGTCTCGGCCTTGGCACCGCAAGAGGAGGGGTTCCCTCTGCGCGGATTGCTGCCTACAAAGTATGTTGGGAAGATGGGTGCTCGGACGCCGACATTCTTGCAGCATTCGACGATGCAATTGCTGATGGGGTCGATATCGTATCTCTTTCGGTTGGGTCGAGTAAACCCCGGCCTTATTTCAGCGATCCCATAGCTATTGGATCTTTCCATGCAATCAAAAATGGAATATTAACCTCCAATTCGGCTGGAAATGAAGGTCCCAAGGACTTCACCACCACAAGCTTGTCTCCATGGCTTCTTTCTGTCGCTGCAAGCGGCATGGATAGAAAATTTGTCACCCGAGTACAGCTTGGCAATGGAAATATCTATCAGGTAACATTATTTTTCTTCTCTCAAAAAATTTCAGCTTTGATTTTGAAATTATCCCAAAGAGTTATATTTTCCATTACCCAAAATTTCTTTTTAGATATAATTGATAGCAAGCTTGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAATACCTAGTTTAATTGGATAAGATATTAAAGTTTCCAACTTTAATCTGATGCATGTATTTAGTATGATTATGATATAATATTTTGATGAAAGTGCAGGGAATTTCAATCAACACATTTAATATGAATTTACAACATCCCTTAGTTTATGCTGGTGATATACCCAACCTAGGTTTCAATGGCTCCACCTCTAGGTAATTTAATTAATGTGTCAGTTTAATTTTTTTTTAGTATTTTTGAAAAAGTAAACTTATGTAAACCTGTTTTACTTTATTGTAGATACTGTGAACAAAATTCAGTGAATCCGATTTTGGCGGCAGGGAAGATCCTTCTTTGCGATTCTGTAATTCCTCCTTCTACATTCTCTTCTTTCGGTGCCATAGCTGGCATCCTAATGCAAGCCTATACGAGGGATAATGCAAGGTCCTATCCCGCACCTGCCGCCGTCCTCGATCCAAATGATGGCATTGCCGTTTATCGTTACATTCGCTCCACAATGTATGTTTCACTTTTTTCAATTAAAATTACCCTTCCAATTCCGTCATGGAACCATTATTCAGTAATTTAAAATTTTGATCAACAGCATTCCCACTGCAACCATCTTCAAGAGTGTCGCTGGACAAGATTCATCTGCTCCTGTTGTAGTTTCCTTCTCATCTCGTGGACCCAATGCTCTAACCAACGACATTCTCAAGGTTAAGTTTTATTCAACCCAAATTATAAAGTTCAAAAAAAAAAAAAAAAGTTCAATTATTCATTAGTTTCATGAATATGCACAAATGCAGCCAGATTTGAGTGGTCCAGGGGTTGAAATTCTAGCAGCATGGCCTCCAGTTGCGCCAATAGCTGGAGTTAAAGACTCTAGGAGTTCGCTTTATAACATAATCTCAGGGACCTCAATGTCTTGTCCACATCTCACTGGAGTTGCAGTCTACGTCAAAACATTCAATCCAACCTTGTCTCCTGCTGCTATAAAATCAGCACTCATGACAACCGGTAATTATTAGTAACTGCTCTGTTTTTCTAAATTTTGTGTGCTTTTCACTCACTCCTCTGTTTCTACAGCTTCGCCCATGAGTGCTAGGCAACATTCACAGGCGGAGTTCGCGTATGGCGCAGGGCATGTCAACCCACTAAAGGCTATAAATCCTGGGTTGGTGTACGATGCGAATGAAAGCGACTACGTGAGCTTCTTGTGCGGTCAAGGTTACAGCACCTCCATGGTTCGACGCATCACTGGCGATAATAGCGCTTGCACTTCTGGCAACATTGGAAGAGTATGGGATCTAAACTATCCTTCTTTTGCTGTTTCGATAAGCGGTACGCGAACCGTGAATCAGTACTTCACAAGAACTCTCACAAACGTTGCGCGGGGAGGATCCACATATAGAGCTAAGATCTCTGCCCCACAAGGGCTTTCCATCACCGTGAATCCTACTGCTCTATCATTCAATGGAATTGGAGATAGGAAATCCTTCAGGCTCACAGTTGGAGGAACAGTGAGTCAGTCCATATCCTCTGCTTCTTTGGAGTGGAGTGATGGTGTTCACTCCGTGAGAAGCCCGATCACTGTGGTTGCTCTCACTAAAACTATAGCTTAA

mRNA sequence

ATGTGTTCTTCTCTGCTCTTAAATCTCCTCTTCCTTGCCCTCTTTTGCAGTGTGCTGGCATCCACCTCCCATTCCCACGATGATGATAGGAAGATATATATTGTGTACATGGGGAGCAAGCTACAGGACCCTTCTTCCGCTCATTTACATCACCGAGCAATGTTGGAACAAGTGGTTGGCAGCACTTTTGATCCACAATCTGTGTTATACACCTACAATAGAAGTTTCAACGGATTCGTGGTGAGACTCACTGAAGAAGAAGCTCAAGAGATTGCTAGCAAGGAGGGTGTGGTCTCTGTGTTCCTCAATCAAAACAACCATCTCCACACGACAAGATCATGGGATTTTATGGGGTTTCCACAAAATGTTGGTCGCGTACAACAACTGGAAAGCAACATAGTTGTTGGAGTTTTGGACAGCGGAATCTGGCCAGAGTCTCCCAGTTTCAACGATGATGGTTTTGGTTCTCCACCATCCAAGTGGAGGGGCAGATGCCAAGACTCCAATTTCAGTTGCAATAGAAAACTTATCGGAGGTCGAGCATATCACATCGGCAGACCCATTCCCCCAGGCGATGTTGGTAGTCCAAGAGATACCGACGGCCATGGCACGCACACTGCATCCACGGTGGCTGGTGGTCTCGTGAGCCAGGCAAGTCTGTATGGTCTCGGCCTTGGCACCGCAAGAGGAGGGGTTCCCTCTGCGCGGATTGCTGCCTACAAAGTATGTTGGGAAGATGGGTGCTCGGACGCCGACATTCTTGCAGCATTCGACGATGCAATTGCTGATGGGGTCGATATCGTATCTCTTTCGGTTGGGTCGAGTAAACCCCGGCCTTATTTCAGCGATCCCATAGCTATTGGATCTTTCCATGCAATCAAAAATGGAATATTAACCTCCAATTCGGCTGGAAATGAAGGTCCCAAGGACTTCACCACCACAAGCTTGTCTCCATGGCTTCTTTCTGTCGCTGCAAGCGGCATGGATAGAAAATTTGTCACCCGAGTACAGCTTGGCAATGGAAATATCTATCAGGGAATTTCAATCAACACATTTAATATGAATTTACAACATCCCTTAGTTTATGCTGGTGATATACCCAACCTAGGTTTCAATGGCTCCACCTCTAGATACTGTGAACAAAATTCAGTGAATCCGATTTTGGCGGCAGGGAAGATCCTTCTTTGCGATTCTGTAATTCCTCCTTCTACATTCTCTTCTTTCGGTGCCATAGCTGGCATCCTAATGCAAGCCTATACGAGGGATAATGCAAGGTCCTATCCCGCACCTGCCGCCGTCCTCGATCCAAATGATGGCATTGCCGTTTATCGTTACATTCGCTCCACAATCATTCCCACTGCAACCATCTTCAAGAGTGTCGCTGGACAAGATTCATCTGCTCCTGTTGTAGTTTCCTTCTCATCTCGTGGACCCAATGCTCTAACCAACGACATTCTCAAGCCAGATTTGAGTGGTCCAGGGGTTGAAATTCTAGCAGCATGGCCTCCAGTTGCGCCAATAGCTGGAGTTAAAGACTCTAGGAGTTCGCTTTATAACATAATCTCAGGGACCTCAATGTCTTGTCCACATCTCACTGGAGTTGCAGTCTACGTCAAAACATTCAATCCAACCTTGTCTCCTGCTGCTATAAAATCAGCACTCATGACAACCGCTTCGCCCATGAGTGCTAGGCAACATTCACAGGCGGAGTTCGCGTATGGCGCAGGGCATGTCAACCCACTAAAGGCTATAAATCCTGGGTTGGTGTACGATGCGAATGAAAGCGACTACGTGAGCTTCTTGTGCGGTCAAGGTTACAGCACCTCCATGGTTCGACGCATCACTGGCGATAATAGCGCTTGCACTTCTGGCAACATTGGAAGAGTATGGGATCTAAACTATCCTTCTTTTGCTGTTTCGATAAGCGGTACGCGAACCGTGAATCAGTACTTCACAAGAACTCTCACAAACGTTGCGCGGGGAGGATCCACATATAGAGCTAAGATCTCTGCCCCACAAGGGCTTTCCATCACCGTGAATCCTACTGCTCTATCATTCAATGGAATTGGAGATAGGAAATCCTTCAGGCTCACAGTTGGAGGAACAGTGAGTCAGTCCATATCCTCTGCTTCTTTGGAGTGGAGTGATGGTGTTCACTCCGTGAGAAGCCCGATCACTGTGGTTGCTCTCACTAAAACTATAGCTTAA

Coding sequence (CDS)

ATGTGTTCTTCTCTGCTCTTAAATCTCCTCTTCCTTGCCCTCTTTTGCAGTGTGCTGGCATCCACCTCCCATTCCCACGATGATGATAGGAAGATATATATTGTGTACATGGGGAGCAAGCTACAGGACCCTTCTTCCGCTCATTTACATCACCGAGCAATGTTGGAACAAGTGGTTGGCAGCACTTTTGATCCACAATCTGTGTTATACACCTACAATAGAAGTTTCAACGGATTCGTGGTGAGACTCACTGAAGAAGAAGCTCAAGAGATTGCTAGCAAGGAGGGTGTGGTCTCTGTGTTCCTCAATCAAAACAACCATCTCCACACGACAAGATCATGGGATTTTATGGGGTTTCCACAAAATGTTGGTCGCGTACAACAACTGGAAAGCAACATAGTTGTTGGAGTTTTGGACAGCGGAATCTGGCCAGAGTCTCCCAGTTTCAACGATGATGGTTTTGGTTCTCCACCATCCAAGTGGAGGGGCAGATGCCAAGACTCCAATTTCAGTTGCAATAGAAAACTTATCGGAGGTCGAGCATATCACATCGGCAGACCCATTCCCCCAGGCGATGTTGGTAGTCCAAGAGATACCGACGGCCATGGCACGCACACTGCATCCACGGTGGCTGGTGGTCTCGTGAGCCAGGCAAGTCTGTATGGTCTCGGCCTTGGCACCGCAAGAGGAGGGGTTCCCTCTGCGCGGATTGCTGCCTACAAAGTATGTTGGGAAGATGGGTGCTCGGACGCCGACATTCTTGCAGCATTCGACGATGCAATTGCTGATGGGGTCGATATCGTATCTCTTTCGGTTGGGTCGAGTAAACCCCGGCCTTATTTCAGCGATCCCATAGCTATTGGATCTTTCCATGCAATCAAAAATGGAATATTAACCTCCAATTCGGCTGGAAATGAAGGTCCCAAGGACTTCACCACCACAAGCTTGTCTCCATGGCTTCTTTCTGTCGCTGCAAGCGGCATGGATAGAAAATTTGTCACCCGAGTACAGCTTGGCAATGGAAATATCTATCAGGGAATTTCAATCAACACATTTAATATGAATTTACAACATCCCTTAGTTTATGCTGGTGATATACCCAACCTAGGTTTCAATGGCTCCACCTCTAGATACTGTGAACAAAATTCAGTGAATCCGATTTTGGCGGCAGGGAAGATCCTTCTTTGCGATTCTGTAATTCCTCCTTCTACATTCTCTTCTTTCGGTGCCATAGCTGGCATCCTAATGCAAGCCTATACGAGGGATAATGCAAGGTCCTATCCCGCACCTGCCGCCGTCCTCGATCCAAATGATGGCATTGCCGTTTATCGTTACATTCGCTCCACAATCATTCCCACTGCAACCATCTTCAAGAGTGTCGCTGGACAAGATTCATCTGCTCCTGTTGTAGTTTCCTTCTCATCTCGTGGACCCAATGCTCTAACCAACGACATTCTCAAGCCAGATTTGAGTGGTCCAGGGGTTGAAATTCTAGCAGCATGGCCTCCAGTTGCGCCAATAGCTGGAGTTAAAGACTCTAGGAGTTCGCTTTATAACATAATCTCAGGGACCTCAATGTCTTGTCCACATCTCACTGGAGTTGCAGTCTACGTCAAAACATTCAATCCAACCTTGTCTCCTGCTGCTATAAAATCAGCACTCATGACAACCGCTTCGCCCATGAGTGCTAGGCAACATTCACAGGCGGAGTTCGCGTATGGCGCAGGGCATGTCAACCCACTAAAGGCTATAAATCCTGGGTTGGTGTACGATGCGAATGAAAGCGACTACGTGAGCTTCTTGTGCGGTCAAGGTTACAGCACCTCCATGGTTCGACGCATCACTGGCGATAATAGCGCTTGCACTTCTGGCAACATTGGAAGAGTATGGGATCTAAACTATCCTTCTTTTGCTGTTTCGATAAGCGGTACGCGAACCGTGAATCAGTACTTCACAAGAACTCTCACAAACGTTGCGCGGGGAGGATCCACATATAGAGCTAAGATCTCTGCCCCACAAGGGCTTTCCATCACCGTGAATCCTACTGCTCTATCATTCAATGGAATTGGAGATAGGAAATCCTTCAGGCTCACAGTTGGAGGAACAGTGAGTCAGTCCATATCCTCTGCTTCTTTGGAGTGGAGTGATGGTGTTCACTCCGTGAGAAGCCCGATCACTGTGGTTGCTCTCACTAAAACTATAGCTTAA

Protein sequence

MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGRAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSVRSPITVVALTKTIA
Homology
BLAST of Csor.00g027340 vs. ExPASy Swiss-Prot
Match: Q39547 (Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1)

HSP 1 Score: 1037.7 bits (2682), Expect = 6.2e-302
Identity = 526/733 (71.76%), Postives = 600/733 (81.86%), Query Frame = 0

Query: 1   MCSSLLLNLLFLALFCS-VLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVV 60
           M SSL+  L F +LF S  LAS   S DD + IYIVYMG KL+DP SAHLHHRAMLEQVV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 61  GSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGF 120
           GSTF P+SVL+TY RSFNGF V+LTEEEA++IAS EGVVSVFLN+ N LHTTRSWDF+GF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 121 PQNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDS-NFSCNRKLIG 180
           P  V R  Q+ESNIVVGVLD+GIWPESPSF+D+GF  PP KW+G C+ S NF CNRK+IG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 181 GRAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIA 240
            R+YHIGRPI PGDV  PRDT+GHGTHTAST AGGLVSQA+LYGLGLGTARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 241 AYKVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGIL 300
           AYKVCW DGCSD DILAA+DDAIADGVDI+SLSVG + PR YF D IAIGSFHA++ GIL
Sbjct: 241 AYKVCWNDGCSDTDILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 301 TSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQH 360
           TSNSAGN GP  FTT SLSPWLLSVAAS MDRKFVT+VQ+GNG  +QG+SINTF+ N  +
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQYY 360

Query: 361 PLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCD-SVIPPSTFSSFGAIAGILMQ 420
           PLV   DIPN GF+ STSR+C   SVNP L  GKI++C+ S  P   F S    AG+LM 
Sbjct: 361 PLVSGRDIPNTGFDKSTSRFCTDKSVNPNLLKGKIVVCEASFGPHEFFKSLDGAAGVLMT 420

Query: 421 AYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRG 480
           + TRD A SYP P++VLDPND +A  RYI S   P ATIFKS    ++SAPVVVSFSSRG
Sbjct: 421 SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRG 480

Query: 481 PNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVY 540
           PN  T D++KPD+SGPGVEILAAWP VAP+ G++  R++L+NIISGTSMSCPH+TG+A Y
Sbjct: 481 PNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIATY 540

Query: 541 VKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDY 600
           VKT+NPT SPAAIKSALMTTASPM+AR + QAEFAYG+GHVNPLKA+ PGLVYDANESDY
Sbjct: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600

Query: 601 VSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTN 660
           V FLCGQGY+T  VRRITGD SACTSGN GRVWDLNYPSF +S+S ++T NQYF RTLT+
Sbjct: 601 VKFLCGQGYNTQAVRRITGDYSACTSGNTGRVWDLNYPSFGLSVSPSQTFNQYFNRTLTS 660

Query: 661 VARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDG 720
           VA   STYRA ISAPQGL+I+VNP  LSFNG+GDRKSF LTV G++   + SASL WSDG
Sbjct: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720

Query: 721 VHSVRSPITVVAL 731
           VH VRSPIT+ +L
Sbjct: 721 VHYVRSPITITSL 730

BLAST of Csor.00g027340 vs. ExPASy Swiss-Prot
Match: Q9STF7 (Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 1.6e-172
Identity = 348/744 (46.77%), Postives = 473/744 (63.58%), Query Frame = 0

Query: 3   SSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHL----HHRAMLEQV 62
           S  LL+ +F  L  S   +++   D D+++YIVYMG+    PS        HH ++L+ V
Sbjct: 6   SYCLLSCIFALLVVSF--ASAGKDDQDKQVYIVYMGAL---PSRVDYMPMSHHTSILQDV 65

Query: 63  VGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMG 122
            G +     ++  Y RSFNGF  RLTE E + +AS + VVSVF ++N +L TT SW+FMG
Sbjct: 66  TGESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMG 125

Query: 123 FPQ--NVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-DSNFSCNRK 182
             +     R   +ES+ ++GV+DSGI+PES SF+  GFG PP KW+G C+  +NF+CN K
Sbjct: 126 LKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNK 185

Query: 183 LIGGRAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSA 242
           LIG R Y    P   G   S RD  GHG+HTAS  AG  V   S YGLG GT RGGVP+A
Sbjct: 186 LIGARYY---TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAA 245

Query: 243 RIAAYKVCWEDG---CSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHA 302
           RIA YKVC + G   C+   ILAAFDDAIAD VDI+++S+G+     +  D +AIG+FHA
Sbjct: 246 RIAVYKVC-DPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHA 305

Query: 303 IKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTF 362
           +  GILT N AGN GP+  T  S++PWL +VAAS M+R F+T+V LGNG    G S+N+F
Sbjct: 306 MAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSF 365

Query: 363 NMN-LQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAI 422
           ++N  ++PLVY G   +   + S++ +C    ++     GKI+LCD+   P    + GA+
Sbjct: 366 DLNGKKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAV 425

Query: 423 AGILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVV 482
           A I+   Y  D A  +  P +VL  +D   V  Y+ ST  P A + KS    +  APVV 
Sbjct: 426 ASIVRNPY-EDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVA 485

Query: 483 SFSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHL 542
           S+SSRGPN L +DILKPD++ PG EILAA+ P  P     D+R   Y +ISGTSMSCPH+
Sbjct: 486 SYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVP-PSESDTRHVKYTVISGTSMSCPHV 545

Query: 543 TGVAVYVKTFNPTLSPAAIKSALMTTASPMSAR---QHSQAEFAYGAGHVNPLKAINPGL 602
            GVA Y+KTF+P  SP+ I+SA+MTTA PM+A     +  AEFAYGAGHV+P+ AI+PGL
Sbjct: 546 AGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGL 605

Query: 603 VYDANESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVW-DLNYPSFAVSISGTRTV 662
           VY+AN+SD+++FLCG  Y+   +R I+GD+S+CT      +  +LNYPS +  +SGT+  
Sbjct: 606 VYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPF 665

Query: 663 NQYFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGT--VSQ 722
              F RT+TNV R  +TY+AK+   + L + V P  LS   + ++KSF +TV G    ++
Sbjct: 666 KVTFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAE 725

Query: 723 SISSASLEWSDGVHSVRSPITVVA 730
           ++ SA L WSDGVH VRSPI V A
Sbjct: 726 NLVSAQLIWSDGVHFVRSPIVVYA 736

BLAST of Csor.00g027340 vs. ExPASy Swiss-Prot
Match: Q8L7D2 (Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2 SV=1)

HSP 1 Score: 605.9 bits (1561), Expect = 6.0e-172
Identity = 343/733 (46.79%), Postives = 464/733 (63.30%), Query Frame = 0

Query: 10  LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 69
           L+  L   +L+S S   D+D ++YIVYMGS        P+S H+   ++L+QV G +   
Sbjct: 10  LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69

Query: 70  QSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ--NV 129
             ++ +Y RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDFMG  +  N 
Sbjct: 70  GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129

Query: 130 GRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-DSNFSCNRKLIGGRAY 189
            R   +ES+ ++GV+D+GIWPES SF+D GFG PP KW+G C    NF+CN KLIG R Y
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY 189

Query: 190 HIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKV 249
                         RDT GHGTHTAST AG  V   S +G+G GT RGGVP++RIAAYKV
Sbjct: 190 ---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKV 249

Query: 250 CWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSNS 309
           C + GCS   +L++FDDAIADGVD++++S+G   P  +  DPIAIG+FHA+  GILT +S
Sbjct: 250 CTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 309

Query: 310 AGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNM-NLQHPLV 369
           AGN GPK  T + ++PW+ +VAAS  +R F+T+V LGNG    G S+N F+M   ++PLV
Sbjct: 310 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 369

Query: 370 YAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTR 429
           Y     +   +  T+  C    +N     GKIL+C          S GAIA I+ ++   
Sbjct: 370 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRP 429

Query: 430 DNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNAL 489
           D A ++  PA+ L   D  ++  YI S   P A + K+    + ++PV+ SFSSRGPN +
Sbjct: 430 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTI 489

Query: 490 TNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTF 549
             DILKPD++ PGVEILAA+ P        D+R   Y++ SGTSM+CPH+ GVA YVKTF
Sbjct: 490 AVDILKPDITAPGVEILAAFSPNGE-PSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTF 549

Query: 550 NPTLSPAAIKSALMTTASPMSA--RQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVS 609
            P  SP+ I+SA+MTTA P+ A  R  +  EFAYGAGHV+P+ A+NPGLVY+ +++D+++
Sbjct: 550 YPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIA 609

Query: 610 FLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNV 669
           FLCG  Y++  ++ I+GD   C+  N     +LNYPS +  +SGT  T +  F RTLTNV
Sbjct: 610 FLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNV 669

Query: 670 ARGGSTYRAKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEW 728
               STY++K+ A  G  LSI V P+ L F  + +++SF +TV G+   S+  SSA+L W
Sbjct: 670 GTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIW 728

BLAST of Csor.00g027340 vs. ExPASy Swiss-Prot
Match: Q9FIF8 (Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 8.7e-171
Identity = 325/707 (45.97%), Postives = 454/707 (64.21%), Query Frame = 0

Query: 32  IYIVYMGSKLQDPSSAHLHHRAMLEQVVGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEI 91
           +YIVYMG+  +   S   HH ++L+++VG+      ++ +Y RSFNGF   L++ E+Q++
Sbjct: 32  VYIVYMGTLPEIKYSPPSHHLSILQKLVGTIAASHLLVRSYKRSFNGFAANLSQAESQKL 91

Query: 92  ASKEGVVSVFLNQNNHLHTTRSWDFMGFPQNVGRVQQLESNIVVGVLDSGIWPESPSFND 151
            + + VVSVF ++++ L TTRSWDF+GF +   R    ES+++VGV+DSGIWPES SF+D
Sbjct: 92  QNMKEVVSVFPSKSHELTTTRSWDFVGFGEKARRESVKESDVIVGVIDSGIWPESESFDD 151

Query: 152 DGFGSPPSKWRGRCQDS-NFSCNRKLIGGRAYHIGRPIPPGDVGSPRDTDGHGTHTASTV 211
           +GFG PP KW+G C+    F+CN KLIG R Y+           S RD +GHGTHTAST 
Sbjct: 152 EGFGPPPKKWKGSCKGGLKFACNNKLIGARFYN-------KFADSARDEEGHGTHTASTA 211

Query: 212 AGGLVSQASLYGLGLGTARGGVPSARIAAYKVCWEDGCSDADILAAFDDAIADGVDIVSL 271
           AG  V  AS YGL  GTARGGVPSARIAAYKVC+ + C+D DILAAFDDAIADGVD++S+
Sbjct: 212 AGNAVQAASFYGLAQGTARGGVPSARIAAYKVCF-NRCNDVDILAAFDDAIADGVDVISI 271

Query: 272 SVGSSKPRPYFSDPIAIGSFHAIKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDR 331
           S+ +       +  +AIGSFHA+  GI+T+ SAGN GP   +  ++SPW+++VAASG DR
Sbjct: 272 SISADYVSNLLNASVAIGSFHAMMRGIITAGSAGNNGPDQGSVANVSPWMITVAASGTDR 331

Query: 332 KFVTRVQLGNGNIYQGISINTFNMN-LQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILA 391
           +F+ RV LGNG    GIS+NTFN+N  + P+VY  ++ +   + + + YC    V+  L 
Sbjct: 332 QFIDRVVLGNGKALTGISVNTFNLNGTKFPIVYGQNV-SRNCSQAQAGYCSSGCVDSELV 391

Query: 392 AGKILLCDSVIPPSTFSSFGAIAGILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRST 451
            GKI+LCD  +        GAI  I+      D+A   P PA+ L   D  ++  YI S 
Sbjct: 392 KGKIVLCDDFLGYREAYLAGAIGVIVQNTLLPDSAFVVPFPASSLGFEDYKSIKSYIESA 451

Query: 452 IIPTATIFKSVAGQDSSAPVVVSFSSRGPNALTNDILKPDLSGPGVEILAAWPPVA---P 511
             P A I ++    D  AP V SFSSRGP+ +  ++LKPD+S PG+EILAA+ PVA    
Sbjct: 452 EPPQAEILRTEEIVDREAPYVPSFSSRGPSFVIQNLLKPDVSAPGLEILAAFSPVASPSS 511

Query: 512 IAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTFNPTLSPAAIKSALMTTASPMSARQH 571
               +D RS  Y+++SGTSM+CPH+ GVA YVK+F+P  SP+AIKSA+MTTA+PM+ +++
Sbjct: 512 FLNPEDKRSVRYSVMSGTSMACPHVAGVAAYVKSFHPDWSPSAIKSAIMTTATPMNLKKN 571

Query: 572 SQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFLCGQGYSTSMVRRITGDNSACTSGNI 631
            + EFAYG+G +NP KA +PGLVY+    DY+  LC +G+ ++ +   +G N  C+    
Sbjct: 572 PEQEFAYGSGQINPTKASDPGLVYEVETEDYLKMLCAEGFDSTTLTTTSGQNVTCSERT- 631

Query: 632 GRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARGGSTYRAKISAPQ-GLSITVNPTALS 691
             V DLNYP+    +S     N  F RT+TNV    STY+A +   Q  L I++ P  L 
Sbjct: 632 -EVKDLNYPTMTTFVSSLDPFNVTFKRTVTNVGFPNSTYKASVVPLQPELQISIEPEILR 691

Query: 692 FNGIGDRKSFRLTVGGTVSQ--SISSASLEWSDGVHSVRSPITVVAL 731
           F  + ++KSF +T+ G   +  S  S+S+ WSDG HSVRSPI   ++
Sbjct: 692 FGFLEEKKSFVVTISGKELKDGSFVSSSVVWSDGSHSVRSPIVAYSI 727

BLAST of Csor.00g027340 vs. ExPASy Swiss-Prot
Match: Q9FIG2 (Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 8.7e-171
Identity = 340/740 (45.95%), Postives = 467/740 (63.11%), Query Frame = 0

Query: 3   SSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQ----DPSSAHLHHRAMLEQV 62
           SS LL+ L +    SV A T     DD+++YIVYMGS        P+S H++   +L++V
Sbjct: 7   SSSLLSCLLVLFLSSVSAVT-----DDKQVYIVYMGSLSSRADYTPTSDHMN---ILQEV 66

Query: 63  VGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMG 122
            G +     ++ +Y RSFNGF  RLTE E + +A   GVVSVF N+   L TT SWDFMG
Sbjct: 67  TGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 126

Query: 123 FPQNV--GRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-DSNFSCNRK 182
             + +   R   +ES+ ++GV+DSGI PES SF+D GFG PP KW+G C    NF+CN K
Sbjct: 127 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNK 186

Query: 183 LIGGRAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSA 242
           LIG R Y              RD DGHGTHTAST AG  V  AS +G+G GT RGGVP++
Sbjct: 187 LIGARDY---------TSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPAS 246

Query: 243 RIAAYKVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKN 302
           R+AAYKVC   GCS   +L+AFDDAIADGVD++++S+G      + +DPIAIG+FHA+  
Sbjct: 247 RVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAK 306

Query: 303 GILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNM- 362
           G+LT NSAGN GPK  + + ++PW+L+VAAS  +R FVT+V LGNG    G S+N + M 
Sbjct: 307 GVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMK 366

Query: 363 NLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGI 422
              +PLVY     +   +  ++  CE + V+     GKIL+C          S GA+ G+
Sbjct: 367 GKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAV-GL 426

Query: 423 LMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFS 482
           + +    D A  +P PAA L   D  ++  Y+ ST  P A + K+ A  + ++PV+ SFS
Sbjct: 427 IYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFS 486

Query: 483 SRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGV 542
           SRGPN +  DILKPD++ PGVEILAA+ P A      D+R   Y+++SGTSMSCPH+ GV
Sbjct: 487 SRGPNTIAVDILKPDITAPGVEILAAYSP-AGEPSQDDTRHVKYSVLSGTSMSCPHVAGV 546

Query: 543 AVYVKTFNPTLSPAAIKSALMTTASPMSARQH--SQAEFAYGAGHVNPLKAINPGLVYDA 602
           A YVKTFNP  SP+ I+SA+MTTA P++A     +  EFAYG+GHV+P+ A NPGLVY+ 
Sbjct: 547 AAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYEL 606

Query: 603 NESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYF 662
           ++SD+++FLCG  Y++ +++ I+G+   C+        +LNYPS +  +SG+  T    F
Sbjct: 607 DKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTF 666

Query: 663 TRTLTNVARGGSTYRAKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSI 722
            RTLTNV    STY +K+ A  G  L + + P+ LSF  + +++SF +TV G+   S+  
Sbjct: 667 NRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVP 726

Query: 723 SSASLEWSDGVHSVRSPITV 728
           SSA+L WSDG H+VRSPI V
Sbjct: 727 SSANLIWSDGTHNVRSPIVV 727

BLAST of Csor.00g027340 vs. NCBI nr
Match: KAG6600967.1 (hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1462 bits (3784), Expect = 0.0
Identity = 735/735 (100.00%), Postives = 735/735 (100.00%), Query Frame = 0

Query: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
           MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60

Query: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
           STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP
Sbjct: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120

Query: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR 180
           QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR
Sbjct: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR 180

Query: 181 AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 240
           AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY
Sbjct: 181 AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 240

Query: 241 KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 300
           KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS
Sbjct: 241 KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 300

Query: 301 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL 360
           NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL
Sbjct: 301 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL 360

Query: 361 VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYT 420
           VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYT
Sbjct: 361 VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYT 420

Query: 421 RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA 480
           RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA
Sbjct: 421 RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA 480

Query: 481 LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT 540
           LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT
Sbjct: 481 LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT 540

Query: 541 FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF 600
           FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF
Sbjct: 541 FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF 600

Query: 601 LCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR 660
           LCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR
Sbjct: 601 LCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR 660

Query: 661 GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHS 720
           GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHS
Sbjct: 661 GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHS 720

Query: 721 VRSPITVVALTKTIA 735
           VRSPITVVALTKTIA
Sbjct: 721 VRSPITVVALTKTIA 735

BLAST of Csor.00g027340 vs. NCBI nr
Match: XP_022957348.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1424 bits (3685), Expect = 0.0
Identity = 717/735 (97.55%), Postives = 723/735 (98.37%), Query Frame = 0

Query: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
           M S L+LNLLFLALFCSVLASTSHSHDDDR+IYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 8   MSSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67

Query: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
           STFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP
Sbjct: 68  STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 127

Query: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR 180
           QNV RV QLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ SNFSCNRKLIGGR
Sbjct: 128 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKLIGGR 187

Query: 181 AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 240
           AYHIGRPI PGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY
Sbjct: 188 AYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 247

Query: 241 KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 300
           KVCWEDGC DADILAAFDDAIADGVDIVSLSVGS+KPRPYF+DPIAIGSFHAIKNGILTS
Sbjct: 248 KVCWEDGCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSFHAIKNGILTS 307

Query: 301 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL 360
           NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL
Sbjct: 308 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL 367

Query: 361 VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYT 420
           VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTF SFGAI GILMQAYT
Sbjct: 368 VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGAIVGILMQAYT 427

Query: 421 RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA 480
           RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA
Sbjct: 428 RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA 487

Query: 481 LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT 540
           LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT
Sbjct: 488 LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT 547

Query: 541 FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF 600
           FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF
Sbjct: 548 FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF 607

Query: 601 LCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR 660
           LCGQGY T+MVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR
Sbjct: 608 LCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR 667

Query: 661 GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHS 720
           GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTV GTVSQSISSASLEWSDGVHS
Sbjct: 668 GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRGTVSQSISSASLEWSDGVHS 727

Query: 721 VRSPITVVALTKTIA 735
           VRSPITVVALTK+IA
Sbjct: 728 VRSPITVVALTKSIA 742

BLAST of Csor.00g027340 vs. NCBI nr
Match: XP_023527145.1 (cucumisin-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1405 bits (3636), Expect = 0.0
Identity = 708/735 (96.33%), Postives = 721/735 (98.10%), Query Frame = 0

Query: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
           MCSSLL NLLFLALF SVLASTSHSH DDRKIYIVYMGSKL+DPSSAHLHHRAMLEQVVG
Sbjct: 8   MCSSLLFNLLFLALFRSVLASTSHSHGDDRKIYIVYMGSKLEDPSSAHLHHRAMLEQVVG 67

Query: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
           STFDPQSVLYTY RSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDF+GFP
Sbjct: 68  STFDPQSVLYTYTRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFLGFP 127

Query: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR 180
           QNVGRV QLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRG CQ SNF CNRKLIGGR
Sbjct: 128 QNVGRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGSCQVSNFRCNRKLIGGR 187

Query: 181 AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 240
           AY IGRPI PGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLG+GTARGGVPSARIAAY
Sbjct: 188 AYRIGRPILPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGIGTARGGVPSARIAAY 247

Query: 241 KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 300
           KVCW+DGCSDADILAAFDDAIADGVDIVSLSVGSS+PRPYF+DPIAIGSFHAIKNGILTS
Sbjct: 248 KVCWKDGCSDADILAAFDDAIADGVDIVSLSVGSSEPRPYFNDPIAIGSFHAIKNGILTS 307

Query: 301 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL 360
           NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIY+GISINTF+MNLQ+PL
Sbjct: 308 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYEGISINTFDMNLQYPL 367

Query: 361 VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYT 420
           VYAGDIPNLGFNGSTSRYCE+NSVNP LAAGKILLCDSVIP S FSSFGAIAGILMQAYT
Sbjct: 368 VYAGDIPNLGFNGSTSRYCEKNSVNPSLAAGKILLCDSVIPSSIFSSFGAIAGILMQAYT 427

Query: 421 RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA 480
           RDNARSYPAPAAVL PNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAP+VVSFSSRGPNA
Sbjct: 428 RDNARSYPAPAAVLHPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPLVVSFSSRGPNA 487

Query: 481 LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT 540
           LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT
Sbjct: 488 LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT 547

Query: 541 FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF 600
           FNPTLSPAAIKSAL+TTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF
Sbjct: 548 FNPTLSPAAIKSALITTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF 607

Query: 601 LCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR 660
           LCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR
Sbjct: 608 LCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR 667

Query: 661 GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHS 720
           GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHS
Sbjct: 668 GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHS 727

Query: 721 VRSPITVVALTKTIA 735
           VRSPITVVALTK+IA
Sbjct: 728 VRSPITVVALTKSIA 742

BLAST of Csor.00g027340 vs. NCBI nr
Match: XP_022957349.1 (cucumisin-like [Cucurbita moschata])

HSP 1 Score: 1200 bits (3105), Expect = 0.0
Identity = 598/729 (82.03%), Postives = 649/729 (89.03%), Query Frame = 0

Query: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
           M S L+LNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1   MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60

Query: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
           STFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP
Sbjct: 61  STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120

Query: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR 180
           QNV RV QLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ SNFSCNRK+IG R
Sbjct: 121 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR 180

Query: 181 AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 240
           AYHIG+ I P +V SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARGG PSARIA Y
Sbjct: 181 AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY 240

Query: 241 KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 300
           K+CW+ GCSDADILAAFDDAIADGVD +SLSVGSSKPRPYFSDPIAIGSFHA+K GILTS
Sbjct: 241 KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 300

Query: 301 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFN-MNLQHP 360
           NSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G  +QG+SINTFN M  Q+P
Sbjct: 301 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP 360

Query: 361 LVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAY 420
           LVY GD+PN GF  S SRYC +NSVNP    GK+LLC++++PPS FSS GAI G++MQ+ 
Sbjct: 361 LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG 420

Query: 421 TRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPN 480
           TRDNA SYP PAA++   D I ++R+ R T+ P A  FKS A QD SAPV+ SFSSRGPN
Sbjct: 421 TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 480

Query: 481 ALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVK 540
           +L N ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+TGVAVYVK
Sbjct: 481 SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 540

Query: 541 TFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVS 600
           TFNPTLSPAAIKSALMTTASPM+A  HSQAEFAYGAGHVNPLKA+NPGLVYDA+E++YV 
Sbjct: 541 TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 600

Query: 601 FLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS-GTRTVNQYFTRTLTNV 660
           FLCGQGY T+MVRRITGDNSACTSGNIGRVWDLN PSFA+S++ G++ +NQYFTRTLTNV
Sbjct: 601 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 660

Query: 661 ARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGV 720
           A  GSTYRA ISAP+GL ITVNP  LSFN IG+RKSF LTVGGTVS+ + SA L WSDGV
Sbjct: 661 ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV 720

Query: 721 HSVRSPITV 727
           HSVRSPIT+
Sbjct: 721 HSVRSPITI 729

BLAST of Csor.00g027340 vs. NCBI nr
Match: BBK45496.1 (pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 trichocucumisin [Trichosanthes bracteata])

HSP 1 Score: 1148 bits (2970), Expect = 0.0
Identity = 566/734 (77.11%), Postives = 643/734 (87.60%), Query Frame = 0

Query: 3   SSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGST 62
           SS++  L+ L+L  S+LAS   S DD RKIYIVYMG+KL+DP SAHLHHRAMLE+VVGST
Sbjct: 8   SSVIFKLVLLSLLSSLLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAMLEEVVGST 67

Query: 63  FDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQN 122
           F P+SV+YTY RSFNGF V+LTEEEAQ+IA+KEGVVSVFLNQ NH+HTT SWDF+GFPQ 
Sbjct: 68  FAPESVIYTYKRSFNGFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSWDFLGFPQA 127

Query: 123 VGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDS-NFSCNRKLIGGRA 182
           V R++Q+ESNIVVGVLDSGIWPESPSF D+GFG  PS W+G CQ S NFSCN K+IG RA
Sbjct: 128 VARMRQVESNIVVGVLDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCNNKIIGARA 187

Query: 183 YHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK 242
           Y +G  +PPGDV SPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK
Sbjct: 188 YRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK 247

Query: 243 VCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSN 302
           +CW DGCSDADILAAFDDAIADGV I+SLSVG S+ RPYF+DPIAIG+FHA+K+GILTSN
Sbjct: 248 ICWSDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAMKHGILTSN 307

Query: 303 SAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPLV 362
           SAGNEG K FTTTSLSPWLLSVAAS  DRKFVT VQLGNG IYQG +INTF+MN Q+PLV
Sbjct: 308 SAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFDMNNQYPLV 367

Query: 363 YAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTR 422
           YAG++PN+GFNGSTSR+C  NSVNP L  GKILLCDS++P S FSSFGAIAG+LMQ YT 
Sbjct: 368 YAGEVPNVGFNGSTSRFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAGVLMQGYT- 427

Query: 423 DNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNAL 482
           DNARS+  PA VL P DG  VYRYI ST  PTA I      +D+SAPVV+SFSSRGPNAL
Sbjct: 428 DNARSHSVPATVLHPQDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISFSSRGPNAL 487

Query: 483 TNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTF 542
           TND+LKPD++GPGVEILAAWPP+API+GV++SRS+L+NIISGTSMSCPHL+ VAVYVKTF
Sbjct: 488 TNDLLKPDITGPGVEILAAWPPIAPISGVRESRSTLFNIISGTSMSCPHLSAVAVYVKTF 547

Query: 543 NPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFL 602
           NP+ SPAAIKSALMTTASPM+AR +S+AEFAYG+GHVNPLKA+NPGLVYDA++SDYV FL
Sbjct: 548 NPSWSPAAIKSALMTTASPMTARLNSEAEFAYGSGHVNPLKAVNPGLVYDADQSDYVKFL 607

Query: 603 CGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARG 662
           CGQGY+T+M+RRITGDNSACTS NIGRVWDLNYPSFA+++SG +++NQYFTRTLTNVA  
Sbjct: 608 CGQGYNTAMLRRITGDNSACTSANIGRVWDLNYPSFALAVSGYQSLNQYFTRTLTNVASQ 667

Query: 663 GSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSV 722
            STYRA IS PQGL+ITVNP  LSF+G GDRKSF LTV G+VSQ I S SL WSDGVHSV
Sbjct: 668 ASTYRATISVPQGLTITVNPPVLSFSGKGDRKSFTLTVRGSVSQPIVSGSLVWSDGVHSV 727

Query: 723 RSPITVVALTKTIA 735
           RSP+ VV LTK++A
Sbjct: 728 RSPLVVVVLTKSVA 740

BLAST of Csor.00g027340 vs. ExPASy TrEMBL
Match: A0A6J1GZY5 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1)

HSP 1 Score: 1424 bits (3685), Expect = 0.0
Identity = 717/735 (97.55%), Postives = 723/735 (98.37%), Query Frame = 0

Query: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
           M S L+LNLLFLALFCSVLASTSHSHDDDR+IYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 8   MSSPLILNLLFLALFCSVLASTSHSHDDDREIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 67

Query: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
           STFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP
Sbjct: 68  STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 127

Query: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR 180
           QNV RV QLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ SNFSCNRKLIGGR
Sbjct: 128 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKLIGGR 187

Query: 181 AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 240
           AYHIGRPI PGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY
Sbjct: 188 AYHIGRPIAPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 247

Query: 241 KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 300
           KVCWEDGC DADILAAFDDAIADGVDIVSLSVGS+KPRPYF+DPIAIGSFHAIKNGILTS
Sbjct: 248 KVCWEDGCWDADILAAFDDAIADGVDIVSLSVGSNKPRPYFNDPIAIGSFHAIKNGILTS 307

Query: 301 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL 360
           NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL
Sbjct: 308 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPL 367

Query: 361 VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYT 420
           VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTF SFGAI GILMQAYT
Sbjct: 368 VYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFFSFGAIVGILMQAYT 427

Query: 421 RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA 480
           RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA
Sbjct: 428 RDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNA 487

Query: 481 LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT 540
           LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT
Sbjct: 488 LTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKT 547

Query: 541 FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF 600
           FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF
Sbjct: 548 FNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSF 607

Query: 601 LCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR 660
           LCGQGY T+MVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR
Sbjct: 608 LCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVAR 667

Query: 661 GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHS 720
           GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTV GTVSQSISSASLEWSDGVHS
Sbjct: 668 GGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVRGTVSQSISSASLEWSDGVHS 727

Query: 721 VRSPITVVALTKTIA 735
           VRSPITVVALTK+IA
Sbjct: 728 VRSPITVVALTKSIA 742

BLAST of Csor.00g027340 vs. ExPASy TrEMBL
Match: A0A6J1H1P2 (cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1)

HSP 1 Score: 1200 bits (3105), Expect = 0.0
Identity = 598/729 (82.03%), Postives = 649/729 (89.03%), Query Frame = 0

Query: 1   MCSSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60
           M S L+LNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG
Sbjct: 1   MSSPLILNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVG 60

Query: 61  STFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120
           STFDPQSVLYTYNRSFNGFVV LTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP
Sbjct: 61  STFDPQSVLYTYNRSFNGFVVTLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFP 120

Query: 121 QNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSNFSCNRKLIGGR 180
           QNV RV QLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ SNFSCNRK+IG R
Sbjct: 121 QNVDRVPQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQVSNFSCNRKIIGAR 180

Query: 181 AYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAY 240
           AYHIG+ I P +V SPRDT+GHGTHTAS VAGGLVSQASLY LGLGTARGG PSARIA Y
Sbjct: 181 AYHIGKSIAPDEVNSPRDTNGHGTHTASIVAGGLVSQASLYNLGLGTARGGAPSARIAVY 240

Query: 241 KVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTS 300
           K+CW+ GCSDADILAAFDDAIADGVD +SLSVGSSKPRPYFSDPIAIGSFHA+K GILTS
Sbjct: 241 KICWKHGCSDADILAAFDDAIADGVDFISLSVGSSKPRPYFSDPIAIGSFHAMKKGILTS 300

Query: 301 NSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFN-MNLQHP 360
           NSAGNEGPKD TTTSLSPWLLSVAA+ MDRKFVTRV+LG+G  +QG+SINTFN M  Q+P
Sbjct: 301 NSAGNEGPKDSTTTSLSPWLLSVAATTMDRKFVTRVRLGDGRFFQGVSINTFNTMVYQYP 360

Query: 361 LVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAY 420
           LVY GD+PN GF  S SRYC +NSVNP    GK+LLC++++PPS FSS GAI G++MQ+ 
Sbjct: 361 LVYGGDVPNRGFKRSISRYCRKNSVNPEKVRGKVLLCEAIVPPSLFSSLGAINGVIMQSG 420

Query: 421 TRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPN 480
           TRDNA SYP PAA++   D I ++R+ R T+ P A  FKS A QD SAPV+ SFSSRGPN
Sbjct: 421 TRDNAGSYPLPAALVSRQDVITIFRFTRETLTPYAMFFKSNATQDPSAPVIASFSSRGPN 480

Query: 481 ALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVK 540
           +L N ILKPDLSGPGVEIL AWPPVAPIA VKDSRSSLYNIISGTSMSC H+TGVAVYVK
Sbjct: 481 SLANHILKPDLSGPGVEILGAWPPVAPIAEVKDSRSSLYNIISGTSMSCSHITGVAVYVK 540

Query: 541 TFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVS 600
           TFNPTLSPAAIKSALMTTASPM+A  HSQAEFAYGAGHVNPLKA+NPGLVYDA+E++YV 
Sbjct: 541 TFNPTLSPAAIKSALMTTASPMNASLHSQAEFAYGAGHVNPLKAVNPGLVYDADENEYVK 600

Query: 601 FLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSIS-GTRTVNQYFTRTLTNV 660
           FLCGQGY T+MVRRITGDNSACTSGNIGRVWDLN PSFA+S++ G++ +NQYFTRTLTNV
Sbjct: 601 FLCGQGYDTAMVRRITGDNSACTSGNIGRVWDLNLPSFALSVAAGSKALNQYFTRTLTNV 660

Query: 661 ARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGV 720
           A  GSTYRA ISAP+GL ITVNP  LSFN IG+RKSF LTVGGTVS+ + SA L WSDGV
Sbjct: 661 ASSGSTYRATISAPKGLRITVNPCVLSFNRIGERKSFNLTVGGTVSRRLVSACLVWSDGV 720

Query: 721 HSVRSPITV 727
           HSVRSPIT+
Sbjct: 721 HSVRSPITI 729

BLAST of Csor.00g027340 vs. ExPASy TrEMBL
Match: A0A4P2YW59 (Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2 SV=1)

HSP 1 Score: 1148 bits (2970), Expect = 0.0
Identity = 566/734 (77.11%), Postives = 643/734 (87.60%), Query Frame = 0

Query: 3   SSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGST 62
           SS++  L+ L+L  S+LAS   S DD RKIYIVYMG+KL+DP SAHLHHRAMLE+VVGST
Sbjct: 8   SSVIFKLVLLSLLSSLLASGFDSDDDGRKIYIVYMGNKLEDPDSAHLHHRAMLEEVVGST 67

Query: 63  FDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQN 122
           F P+SV+YTY RSFNGF V+LTEEEAQ+IA+KEGVVSVFLNQ NH+HTT SWDF+GFPQ 
Sbjct: 68  FAPESVIYTYKRSFNGFAVKLTEEEAQKIAAKEGVVSVFLNQQNHIHTTNSWDFLGFPQA 127

Query: 123 VGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDS-NFSCNRKLIGGRA 182
           V R++Q+ESNIVVGVLDSGIWPESPSF D+GFG  PS W+G CQ S NFSCN K+IG RA
Sbjct: 128 VARMRQVESNIVVGVLDSGIWPESPSFRDEGFGRAPSSWKGTCQASKNFSCNNKIIGARA 187

Query: 183 YHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK 242
           Y +G  +PPGDV SPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK
Sbjct: 188 YRVGGTLPPGDVSSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK 247

Query: 243 VCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSN 302
           +CW DGCSDADILAAFDDAIADGV I+SLSVG S+ RPYF+DPIAIG+FHA+K+GILTSN
Sbjct: 248 ICWSDGCSDADILAAFDDAIADGVHIISLSVGGSQARPYFNDPIAIGAFHAMKHGILTSN 307

Query: 303 SAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPLV 362
           SAGNEG K FTTTSLSPWLLSVAAS  DRKFVT VQLGNG IYQG +INTF+MN Q+PLV
Sbjct: 308 SAGNEGSKFFTTTSLSPWLLSVAASTTDRKFVTHVQLGNGKIYQGTAINTFDMNNQYPLV 367

Query: 363 YAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTR 422
           YAG++PN+GFNGSTSR+C  NSVNP L  GKILLCDS++P S FSSFGAIAG+LMQ YT 
Sbjct: 368 YAGEVPNVGFNGSTSRFCLNNSVNPYLVKGKILLCDSILPSSVFSSFGAIAGVLMQGYT- 427

Query: 423 DNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNAL 482
           DNARS+  PA VL P DG  VYRYI ST  PTA I      +D+SAPVV+SFSSRGPNAL
Sbjct: 428 DNARSHSVPATVLHPQDGANVYRYIYSTSTPTARILSGTTVRDASAPVVISFSSRGPNAL 487

Query: 483 TNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTF 542
           TND+LKPD++GPGVEILAAWPP+API+GV++SRS+L+NIISGTSMSCPHL+ VAVYVKTF
Sbjct: 488 TNDLLKPDITGPGVEILAAWPPIAPISGVRESRSTLFNIISGTSMSCPHLSAVAVYVKTF 547

Query: 543 NPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFL 602
           NP+ SPAAIKSALMTTASPM+AR +S+AEFAYG+GHVNPLKA+NPGLVYDA++SDYV FL
Sbjct: 548 NPSWSPAAIKSALMTTASPMTARLNSEAEFAYGSGHVNPLKAVNPGLVYDADQSDYVKFL 607

Query: 603 CGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARG 662
           CGQGY+T+M+RRITGDNSACTS NIGRVWDLNYPSFA+++SG +++NQYFTRTLTNVA  
Sbjct: 608 CGQGYNTAMLRRITGDNSACTSANIGRVWDLNYPSFALAVSGYQSLNQYFTRTLTNVASQ 667

Query: 663 GSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSV 722
            STYRA IS PQGL+ITVNP  LSF+G GDRKSF LTV G+VSQ I S SL WSDGVHSV
Sbjct: 668 ASTYRATISVPQGLTITVNPPVLSFSGKGDRKSFTLTVRGSVSQPIVSGSLVWSDGVHSV 727

Query: 723 RSPITVVALTKTIA 735
           RSP+ VV LTK++A
Sbjct: 728 RSPLVVVVLTKSVA 740

BLAST of Csor.00g027340 vs. ExPASy TrEMBL
Match: K7NBW1 (Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1)

HSP 1 Score: 1094 bits (2830), Expect = 0.0
Identity = 545/729 (74.76%), Postives = 625/729 (85.73%), Query Frame = 0

Query: 3   SSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVVGST 62
           SSL+  L+FL+LFCS+L S+S S+DD RKIYIVYMGSKL+D +SAHL+HRAMLE+VVGST
Sbjct: 7   SSLVFKLIFLSLFCSLLVSSSDSNDDGRKIYIVYMGSKLEDTASAHLYHRAMLEEVVGST 66

Query: 63  FDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQN 122
           F P+SV+YTY RSFNGF V+LTEEEA +IA+KEGVVSVF ++ NHLHTTRSWDF+G  QN
Sbjct: 67  FAPESVIYTYKRSFNGFAVKLTEEEALKIAAKEGVVSVFPSEKNHLHTTRSWDFLGISQN 126

Query: 123 VGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDS-NFSCNRKLIGGRA 182
           V RV+Q+ESNIVVGV DSGIWPE+PSFNDDGFG  P+ WRG CQ S NF CNRK+IG RA
Sbjct: 127 VPRVKQVESNIVVGVFDSGIWPENPSFNDDGFGPAPANWRGTCQASTNFRCNRKIIGARA 186

Query: 183 YHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYK 242
           Y     +PPGDV SPRDTDGHGTHTASTVAG LVSQASLYGLG+GTARGGVP ARIA YK
Sbjct: 187 YRSST-LPPGDVRSPRDTDGHGTHTASTVAGVLVSQASLYGLGVGTARGGVPPARIAVYK 246

Query: 243 VCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSN 302
           +CW DGCSDADILAAFDDAIADGVDI+SLSVG   P+PY  + IAIGSFHA+K GILTSN
Sbjct: 247 ICWSDGCSDADILAAFDDAIADGVDIISLSVGGKVPQPYLYNSIAIGSFHAMKRGILTSN 306

Query: 303 SAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQHPLV 362
           SAGN GPK FT TSLSPWL +VAAS  DRKFVT+V LGNGN YQG+SINTF+M  Q+PL+
Sbjct: 307 SAGNNGPKSFTVTSLSPWLPTVAASSSDRKFVTQVLLGNGNTYQGVSINTFDMRNQYPLI 366

Query: 363 YAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTR 422
           YAG+ P++GFN STSRYC ++SV+P L  GKILLCDS   P+ F+SFG  AG+LMQ+ TR
Sbjct: 367 YAGNAPSIGFNSSTSRYCYEDSVDPNLVRGKILLCDSTFGPTVFASFGGAAGVLMQSNTR 426

Query: 423 DNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNAL 482
           D+A SYP PA+VLDP  G  + RY+ ST  PTATIFKS   +D+SAPVVVSFSSRGPN +
Sbjct: 427 DHASSYPLPASVLDPAGGNNIKRYMSSTRAPTATIFKSTVVRDTSAPVVVSFSSRGPNYV 486

Query: 483 TNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTF 542
           T+DILKPD + PGVEILAAWPPVAPI+GV+DSRS+LYNIISGTSMSCPH+T +AV++KTF
Sbjct: 487 THDILKPDSTAPGVEILAAWPPVAPISGVRDSRSALYNIISGTSMSCPHVTAIAVHIKTF 546

Query: 543 NPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFL 602
            P+ SPAAIKSALMTTASPM+AR +S AEFAYG+GHVNPLKA++PGLVYDA+ESDYV FL
Sbjct: 547 YPSWSPAAIKSALMTTASPMNARFNSDAEFAYGSGHVNPLKAVDPGLVYDASESDYVKFL 606

Query: 603 CGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTNVARG 662
           CG+GY+T+MVR  TGDNSACTSGNIGRVWDLNYPSFA+SIS ++T NQ F RTLTNV  G
Sbjct: 607 CGEGYTTAMVRSTTGDNSACTSGNIGRVWDLNYPSFALSISRSQTANQSFRRTLTNVVSG 666

Query: 663 GSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDGVHSV 722
            STYRA ISAPQGLSI+VNP+ LSFNGIGD+KSF LTV GTVSQ+I SASL WSDG H+V
Sbjct: 667 ASTYRASISAPQGLSISVNPSVLSFNGIGDQKSFTLTVRGTVSQAIVSASLVWSDGSHNV 726

Query: 723 RSPITVVAL 730
           RSPITV  L
Sbjct: 727 RSPITVYVL 734

BLAST of Csor.00g027340 vs. ExPASy TrEMBL
Match: A0A1S3CF86 (LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1)

HSP 1 Score: 1038 bits (2684), Expect = 0.0
Identity = 526/733 (71.76%), Postives = 601/733 (81.99%), Query Frame = 0

Query: 1   MCSSLLLNLLFLALFCSV-LASTSHSHDDDRKIYIVYMGSKLQDPSSAHLHHRAMLEQVV 60
           M SSL+  L F +LF S  LAS   S DD + IYIVYMG KL+DP SAHLHHRAMLEQVV
Sbjct: 1   MSSSLIFKLFFFSLFFSNRLASRLDSDDDGKNIYIVYMGRKLEDPDSAHLHHRAMLEQVV 60

Query: 61  GSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGF 120
           GSTF P+SVL+TY RSFNGF V+LTEEEA++IAS EGVVSVFLN+ N LHTTRSWDF+GF
Sbjct: 61  GSTFAPESVLHTYKRSFNGFAVKLTEEEAEKIASMEGVVSVFLNEMNELHTTRSWDFLGF 120

Query: 121 PQNVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQDSN-FSCNRKLIG 180
           P  V R  Q+ESNIVVGVLD+GIWPESPSF+D+GF  PP KW+G C+ SN F CNRK+IG
Sbjct: 121 PLTVPRRSQVESNIVVGVLDTGIWPESPSFDDEGFSPPPPKWKGTCETSNNFRCNRKIIG 180

Query: 181 GRAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIA 240
            R+YHIGRPI PGDV  PRDT+GHGTHTAST AGGLVSQA+LYGLGLGTARGGVP ARIA
Sbjct: 181 ARSYHIGRPISPGDVNGPRDTNGHGTHTASTAAGGLVSQANLYGLGLGTARGGVPLARIA 240

Query: 241 AYKVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGIL 300
           AYKVCW DGCSDADILAA+DDAIADGVDI+SLSVG + PR YF D IAIGSFHA++ GIL
Sbjct: 241 AYKVCWNDGCSDADILAAYDDAIADGVDIISLSVGGANPRHYFVDAIAIGSFHAVERGIL 300

Query: 301 TSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNMNLQH 360
           TSNSAGN GP  FTT SLSPWLLSVAAS MDRKFVT+VQ+GNG  +QG+SINTF+ N  +
Sbjct: 301 TSNSAGNGGPNFFTTASLSPWLLSVAASTMDRKFVTQVQIGNGQSFQGVSINTFD-NQYY 360

Query: 361 PLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTF-SSFGAIAGILMQ 420
           PLV   DIPN GF+ STSR+C  NSV P L  GKI++C++   P  F  S    AG+LM 
Sbjct: 361 PLVSGRDIPNPGFDKSTSRFCTDNSVXPKLLKGKIVVCEASFGPHEFFKSLDGAAGVLMT 420

Query: 421 AYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRG 480
           + TRD A SYP P++VLDPND +A  RYI S   P ATIFKS    ++SAPVVVSFSSRG
Sbjct: 421 SNTRDYADSYPLPSSVLDPNDLLATLRYIYSIRSPGATIFKSTTILNASAPVVVSFSSRG 480

Query: 481 PNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVY 540
           PN  T D++KPD+SGPGVEILAAWP VAP+ G++  R++L+NIISGTSMSCPH+TG+A Y
Sbjct: 481 PNRATKDVIKPDISGPGVEILAAWPSVAPVGGIR--RNTLFNIISGTSMSCPHITGIATY 540

Query: 541 VKTFNPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDY 600
           VKT+NPT SPAAIKSALMTTASPM+AR + QAEFAYG+GHVNPLKA+ PGLVYDANESDY
Sbjct: 541 VKTYNPTWSPAAIKSALMTTASPMNARFNPQAEFAYGSGHVNPLKAVRPGLVYDANESDY 600

Query: 601 VSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGTRTVNQYFTRTLTN 660
           V FLCGQGY+T  VRRITGD SACT GN GRVWDLNYPSF +S+S ++T NQYF RTLT+
Sbjct: 601 VKFLCGQGYNTEAVRRITGDYSACTPGNTGRVWDLNYPSFGLSVSPSKTFNQYFNRTLTS 660

Query: 661 VARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGTVSQSISSASLEWSDG 720
           VA   STYRA ISAPQGL+I+VNP  LSFNG+GDRKSF LTV G++   + SASL WSDG
Sbjct: 661 VAPQASTYRAMISAPQGLTISVNPNVLSFNGLGDRKSFTLTVRGSIKGFVVSASLVWSDG 720

Query: 721 VHSVRSPITVVAL 730
           VHSVRSPIT+ +L
Sbjct: 721 VHSVRSPITITSL 730

BLAST of Csor.00g027340 vs. TAIR 10
Match: AT3G46850.1 (Subtilase family protein )

HSP 1 Score: 607.8 bits (1566), Expect = 1.1e-173
Identity = 348/744 (46.77%), Postives = 473/744 (63.58%), Query Frame = 0

Query: 3   SSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQDPSSAHL----HHRAMLEQV 62
           S  LL+ +F  L  S   +++   D D+++YIVYMG+    PS        HH ++L+ V
Sbjct: 6   SYCLLSCIFALLVVSF--ASAGKDDQDKQVYIVYMGAL---PSRVDYMPMSHHTSILQDV 65

Query: 63  VGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMG 122
            G +     ++  Y RSFNGF  RLTE E + +AS + VVSVF ++N +L TT SW+FMG
Sbjct: 66  TGESSIQDRLVRNYKRSFNGFAARLTESEREILASMDEVVSVFPSKNLNLQTTTSWNFMG 125

Query: 123 FPQ--NVGRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-DSNFSCNRK 182
             +     R   +ES+ ++GV+DSGI+PES SF+  GFG PP KW+G C+  +NF+CN K
Sbjct: 126 LKEGKRTKRNPLIESDTIIGVIDSGIYPESDSFSGKGFGPPPKKWKGVCKGGTNFTCNNK 185

Query: 183 LIGGRAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSA 242
           LIG R Y    P   G   S RD  GHG+HTAS  AG  V   S YGLG GT RGGVP+A
Sbjct: 186 LIGARYY---TPKLEGFPESARDNTGHGSHTASIAAGNAVKHVSFYGLGNGTVRGGVPAA 245

Query: 243 RIAAYKVCWEDG---CSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHA 302
           RIA YKVC + G   C+   ILAAFDDAIAD VDI+++S+G+     +  D +AIG+FHA
Sbjct: 246 RIAVYKVC-DPGVIRCTSDGILAAFDDAIADKVDIITVSLGADAVGTFEEDTLAIGAFHA 305

Query: 303 IKNGILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTF 362
           +  GILT N AGN GP+  T  S++PWL +VAAS M+R F+T+V LGNG    G S+N+F
Sbjct: 306 MAKGILTVNGAGNNGPERRTIVSMAPWLFTVAASNMNRAFITKVVLGNGKTIVGRSVNSF 365

Query: 363 NMN-LQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAI 422
           ++N  ++PLVY G   +   + S++ +C    ++     GKI+LCD+   P    + GA+
Sbjct: 366 DLNGKKYPLVY-GKSASSRCDASSAGFCSPGCLDSKRVKGKIVLCDTQRNPGEAQAMGAV 425

Query: 423 AGILMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVV 482
           A I+   Y  D A  +  P +VL  +D   V  Y+ ST  P A + KS    +  APVV 
Sbjct: 426 ASIVRNPY-EDAASVFSFPVSVLSEDDYNIVLSYVNSTKNPKAAVLKSETIFNQKAPVVA 485

Query: 483 SFSSRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHL 542
           S+SSRGPN L +DILKPD++ PG EILAA+ P  P     D+R   Y +ISGTSMSCPH+
Sbjct: 486 SYSSRGPNPLIHDILKPDITAPGSEILAAYSPYVP-PSESDTRHVKYTVISGTSMSCPHV 545

Query: 543 TGVAVYVKTFNPTLSPAAIKSALMTTASPMSAR---QHSQAEFAYGAGHVNPLKAINPGL 602
            GVA Y+KTF+P  SP+ I+SA+MTTA PM+A     +  AEFAYGAGHV+P+ AI+PGL
Sbjct: 546 AGVAAYIKTFHPLWSPSMIQSAIMTTAWPMNASTSPSNELAEFAYGAGHVDPIAAIHPGL 605

Query: 603 VYDANESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVW-DLNYPSFAVSISGTRTV 662
           VY+AN+SD+++FLCG  Y+   +R I+GD+S+CT      +  +LNYPS +  +SGT+  
Sbjct: 606 VYEANKSDHITFLCGFNYTGKKLRLISGDSSSCTKEQTKSLTRNLNYPSMSAQVSGTKPF 665

Query: 663 NQYFTRTLTNVARGGSTYRAKISAPQGLSITVNPTALSFNGIGDRKSFRLTVGGT--VSQ 722
              F RT+TNV R  +TY+AK+   + L + V P  LS   + ++KSF +TV G    ++
Sbjct: 666 KVTFRRTVTNVGRPNATYKAKVVGSK-LKVKVVPAVLSLKSLYEKKSFTVTVSGAGPKAE 725

Query: 723 SISSASLEWSDGVHSVRSPITVVA 730
           ++ SA L WSDGVH VRSPI V A
Sbjct: 726 NLVSAQLIWSDGVHFVRSPIVVYA 736

BLAST of Csor.00g027340 vs. TAIR 10
Match: AT5G59090.1 (subtilase 4.12 )

HSP 1 Score: 605.9 bits (1561), Expect = 4.3e-173
Identity = 343/733 (46.79%), Postives = 464/733 (63.30%), Query Frame = 0

Query: 10  LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 69
           L+  L   +L+S S   D+D ++YIVYMGS        P+S H+   ++L+QV G +   
Sbjct: 10  LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69

Query: 70  QSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ--NV 129
             ++ +Y RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDFMG  +  N 
Sbjct: 70  GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129

Query: 130 GRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-DSNFSCNRKLIGGRAY 189
            R   +ES+ ++GV+D+GIWPES SF+D GFG PP KW+G C    NF+CN KLIG R Y
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY 189

Query: 190 HIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKV 249
                         RDT GHGTHTAST AG  V   S +G+G GT RGGVP++RIAAYKV
Sbjct: 190 ---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKV 249

Query: 250 CWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSNS 309
           C + GCS   +L++FDDAIADGVD++++S+G   P  +  DPIAIG+FHA+  GILT +S
Sbjct: 250 CTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 309

Query: 310 AGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNM-NLQHPLV 369
           AGN GPK  T + ++PW+ +VAAS  +R F+T+V LGNG    G S+N F+M   ++PLV
Sbjct: 310 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 369

Query: 370 YAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTR 429
           Y     +   +  T+  C    +N     GKIL+C          S GAIA I+ ++   
Sbjct: 370 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRP 429

Query: 430 DNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNAL 489
           D A ++  PA+ L   D  ++  YI S   P A + K+    + ++PV+ SFSSRGPN +
Sbjct: 430 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTI 489

Query: 490 TNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTF 549
             DILKPD++ PGVEILAA+ P        D+R   Y++ SGTSM+CPH+ GVA YVKTF
Sbjct: 490 AVDILKPDITAPGVEILAAFSPNGE-PSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTF 549

Query: 550 NPTLSPAAIKSALMTTASPMSA--RQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVS 609
            P  SP+ I+SA+MTTA P+ A  R  +  EFAYGAGHV+P+ A+NPGLVY+ +++D+++
Sbjct: 550 YPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIA 609

Query: 610 FLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNV 669
           FLCG  Y++  ++ I+GD   C+  N     +LNYPS +  +SGT  T +  F RTLTNV
Sbjct: 610 FLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNV 669

Query: 670 ARGGSTYRAKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEW 728
               STY++K+ A  G  LSI V P+ L F  + +++SF +TV G+   S+  SSA+L W
Sbjct: 670 GTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIW 728

BLAST of Csor.00g027340 vs. TAIR 10
Match: AT5G59090.2 (subtilase 4.12 )

HSP 1 Score: 602.4 bits (1552), Expect = 4.7e-172
Identity = 341/731 (46.65%), Postives = 461/731 (63.06%), Query Frame = 0

Query: 10  LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 69
           L+  L   +L+S S   D+D ++YIVYMGS        P+S H+   ++L+QV G +   
Sbjct: 10  LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69

Query: 70  QSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ--NV 129
             ++ +Y RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDFMG  +  N 
Sbjct: 70  GRLVRSYKRSFNGFAARLTESERTLIAEIEGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129

Query: 130 GRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-DSNFSCNRKLIGGRAY 189
            R   +ES+ ++GV+D+GIWPES SF+D GFG PP KW+G C    NF+CN KLIG R Y
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY 189

Query: 190 HIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKV 249
                         RDT GHGTHTAST AG  V   S +G+G GT RGGVP++RIAAYKV
Sbjct: 190 ---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKV 249

Query: 250 CWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSNS 309
           C + GCS   +L++FDDAIADGVD++++S+G   P  +  DPIAIG+FHA+  GILT +S
Sbjct: 250 CTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 309

Query: 310 AGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNM-NLQHPLV 369
           AGN GPK  T + ++PW+ +VAAS  +R F+T+V LGNG    G S+N F+M   ++PLV
Sbjct: 310 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 369

Query: 370 YAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTR 429
           Y     +   +  T+  C    +N     GKIL+C          S GAIA I+ ++   
Sbjct: 370 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRP 429

Query: 430 DNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNAL 489
           D A ++  PA+ L   D  ++  YI S   P A + K+    + ++PV+ SFSSRGPN +
Sbjct: 430 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTI 489

Query: 490 TNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTF 549
             DILKPD++ PGVEILAA+ P        D+R   Y++ SGTSM+CPH+ GVA YVKTF
Sbjct: 490 AVDILKPDITAPGVEILAAFSPNGE-PSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTF 549

Query: 550 NPTLSPAAIKSALMTTASPMSARQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVSFL 609
            P  SP+ I+SA+MTTA     R  +  EFAYGAGHV+P+ A+NPGLVY+ +++D+++FL
Sbjct: 550 YPRWSPSMIQSAIMTTA---KGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIAFL 609

Query: 610 CGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNVAR 669
           CG  Y++  ++ I+GD   C+  N     +LNYPS +  +SGT  T +  F RTLTNV  
Sbjct: 610 CGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNVGT 669

Query: 670 GGSTYRAKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEWSD 728
             STY++K+ A  G  LSI V P+ L F  + +++SF +TV G+   S+  SSA+L WSD
Sbjct: 670 PNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIWSD 723

BLAST of Csor.00g027340 vs. TAIR 10
Match: AT5G59120.1 (subtilase 4.13 )

HSP 1 Score: 602.1 bits (1551), Expect = 6.2e-172
Identity = 340/740 (45.95%), Postives = 467/740 (63.11%), Query Frame = 0

Query: 3   SSLLLNLLFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQ----DPSSAHLHHRAMLEQV 62
           SS LL+ L +    SV A T     DD+++YIVYMGS        P+S H++   +L++V
Sbjct: 7   SSSLLSCLLVLFLSSVSAVT-----DDKQVYIVYMGSLSSRADYTPTSDHMN---ILQEV 66

Query: 63  VGSTFDPQSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMG 122
            G +     ++ +Y RSFNGF  RLTE E + +A   GVVSVF N+   L TT SWDFMG
Sbjct: 67  TGESSIEGRLVRSYKRSFNGFAARLTESERERVAKMVGVVSVFPNKKLQLQTTTSWDFMG 126

Query: 123 FPQNV--GRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-DSNFSCNRK 182
             + +   R   +ES+ ++GV+DSGI PES SF+D GFG PP KW+G C    NF+CN K
Sbjct: 127 LKEGIKTKRNPTVESDTIIGVIDSGITPESQSFSDKGFGPPPQKWKGVCSGGKNFTCNNK 186

Query: 183 LIGGRAYHIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSA 242
           LIG R Y              RD DGHGTHTAST AG  V  AS +G+G GT RGGVP++
Sbjct: 187 LIGARDY---------TSEGTRDMDGHGTHTASTAAGNAVVDASFFGIGNGTVRGGVPAS 246

Query: 243 RIAAYKVCWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKN 302
           R+AAYKVC   GCS   +L+AFDDAIADGVD++++S+G      + +DPIAIG+FHA+  
Sbjct: 247 RVAAYKVCTPTGCSSEALLSAFDDAIADGVDLITISIGDKTASMFQNDPIAIGAFHAMAK 306

Query: 303 GILTSNSAGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNM- 362
           G+LT NSAGN GPK  + + ++PW+L+VAAS  +R FVT+V LGNG    G S+N + M 
Sbjct: 307 GVLTVNSAGNSGPKPISVSGVAPWILTVAASTTNRGFVTKVVLGNGKTLVGKSVNAYEMK 366

Query: 363 NLQHPLVYAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGI 422
              +PLVY     +   +  ++  CE + V+     GKIL+C          S GA+ G+
Sbjct: 367 GKDYPLVYGKSAASSACDAESAGLCELSCVDKSRVKGKILVCGGPGGLKIVESVGAV-GL 426

Query: 423 LMQAYTRDNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFS 482
           + +    D A  +P PAA L   D  ++  Y+ ST  P A + K+ A  + ++PV+ SFS
Sbjct: 427 IYRTPKPDVAFIHPLPAAGLLTEDFESLVSYLESTDSPQAIVLKTEAIFNRTSPVIASFS 486

Query: 483 SRGPNALTNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGV 542
           SRGPN +  DILKPD++ PGVEILAA+ P A      D+R   Y+++SGTSMSCPH+ GV
Sbjct: 487 SRGPNTIAVDILKPDITAPGVEILAAYSP-AGEPSQDDTRHVKYSVLSGTSMSCPHVAGV 546

Query: 543 AVYVKTFNPTLSPAAIKSALMTTASPMSARQH--SQAEFAYGAGHVNPLKAINPGLVYDA 602
           A YVKTFNP  SP+ I+SA+MTTA P++A     +  EFAYG+GHV+P+ A NPGLVY+ 
Sbjct: 547 AAYVKTFNPKWSPSMIQSAIMTTAWPVNATGTGIASTEFAYGSGHVDPIAASNPGLVYEL 606

Query: 603 NESDYVSFLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYF 662
           ++SD+++FLCG  Y++ +++ I+G+   C+        +LNYPS +  +SG+  T    F
Sbjct: 607 DKSDHIAFLCGMNYTSQVLKVISGETVTCSEAKKILPRNLNYPSMSAKLSGSGTTFTVTF 666

Query: 663 TRTLTNVARGGSTYRAKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSI 722
            RTLTNV    STY +K+ A  G  L + + P+ LSF  + +++SF +TV G+   S+  
Sbjct: 667 NRTLTNVGTPNSTYTSKVVAGHGSKLDVKITPSVLSFKTVNEKQSFTVTVTGSNLDSEVP 726

Query: 723 SSASLEWSDGVHSVRSPITV 728
           SSA+L WSDG H+VRSPI V
Sbjct: 727 SSANLIWSDGTHNVRSPIVV 727

BLAST of Csor.00g027340 vs. TAIR 10
Match: AT5G59090.3 (subtilase 4.12 )

HSP 1 Score: 602.1 bits (1551), Expect = 6.2e-172
Identity = 343/733 (46.79%), Postives = 464/733 (63.30%), Query Frame = 0

Query: 10  LFLALFCSVLASTSHSHDDDRKIYIVYMGSKLQD----PSSAHLHHRAMLEQVVGSTFDP 69
           L+  L   +L+S S   D+D ++YIVYMGS        P+S H+   ++L+QV G +   
Sbjct: 10  LYSWLLVLLLSSVSAIIDEDTQVYIVYMGSLSSRADYIPTSDHM---SILQQVTGESSIE 69

Query: 70  QSVLYTYNRSFNGFVVRLTEEEAQEIASKEGVVSVFLNQNNHLHTTRSWDFMGFPQ--NV 129
             ++ +Y RSFNGF  RLTE E   IA  EGVVSVF N+   LHTT SWDFMG  +  N 
Sbjct: 70  GRLVRSYKRSFNGFAARLTESERTLIA--EGVVSVFPNKILQLHTTTSWDFMGVKEGKNT 129

Query: 130 GRVQQLESNIVVGVLDSGIWPESPSFNDDGFGSPPSKWRGRCQ-DSNFSCNRKLIGGRAY 189
            R   +ES+ ++GV+D+GIWPES SF+D GFG PP KW+G C    NF+CN KLIG R Y
Sbjct: 130 KRNLAIESDTIIGVIDTGIWPESKSFSDKGFGPPPKKWKGVCSGGKNFTCNNKLIGARDY 189

Query: 190 HIGRPIPPGDVGSPRDTDGHGTHTASTVAGGLVSQASLYGLGLGTARGGVPSARIAAYKV 249
                         RDT GHGTHTAST AG  V   S +G+G GT RGGVP++RIAAYKV
Sbjct: 190 ---------TSEGTRDTSGHGTHTASTAAGNAVKDTSFFGIGNGTVRGGVPASRIAAYKV 249

Query: 250 CWEDGCSDADILAAFDDAIADGVDIVSLSVGSSKPRPYFSDPIAIGSFHAIKNGILTSNS 309
           C + GCS   +L++FDDAIADGVD++++S+G   P  +  DPIAIG+FHA+  GILT +S
Sbjct: 250 CTDSGCSSEALLSSFDDAIADGVDLITISIGFQFPSIFEDDPIAIGAFHAMAKGILTVSS 309

Query: 310 AGNEGPKDFTTTSLSPWLLSVAASGMDRKFVTRVQLGNGNIYQGISINTFNM-NLQHPLV 369
           AGN GPK  T + ++PW+ +VAAS  +R F+T+V LGNG    G S+N F+M   ++PLV
Sbjct: 310 AGNSGPKPTTVSHVAPWIFTVAASTTNRGFITKVVLGNGKTLAGRSVNAFDMKGKKYPLV 369

Query: 370 YAGDIPNLGFNGSTSRYCEQNSVNPILAAGKILLCDSVIPPSTFSSFGAIAGILMQAYTR 429
           Y     +   +  T+  C    +N     GKIL+C          S GAIA I+ ++   
Sbjct: 370 YGKSAASSACDAKTAALCAPACLNKSRVKGKILVCGGPSGYKIAKSVGAIA-IIDKSPRP 429

Query: 430 DNARSYPAPAAVLDPNDGIAVYRYIRSTIIPTATIFKSVAGQDSSAPVVVSFSSRGPNAL 489
           D A ++  PA+ L   D  ++  YI S   P A + K+    + ++PV+ SFSSRGPN +
Sbjct: 430 DVAFTHHLPASGLKAKDFKSLVSYIESQDSPQAAVLKTETIFNRTSPVIASFSSRGPNTI 489

Query: 490 TNDILKPDLSGPGVEILAAWPPVAPIAGVKDSRSSLYNIISGTSMSCPHLTGVAVYVKTF 549
             DILKPD++ PGVEILAA+ P        D+R   Y++ SGTSM+CPH+ GVA YVKTF
Sbjct: 490 AVDILKPDITAPGVEILAAFSPNGE-PSEDDTRRVKYSVFSGTSMACPHVAGVAAYVKTF 549

Query: 550 NPTLSPAAIKSALMTTASPMSA--RQHSQAEFAYGAGHVNPLKAINPGLVYDANESDYVS 609
            P  SP+ I+SA+MTTA P+ A  R  +  EFAYGAGHV+P+ A+NPGLVY+ +++D+++
Sbjct: 550 YPRWSPSMIQSAIMTTAWPVKAKGRGIASTEFAYGAGHVDPMAALNPGLVYELDKADHIA 609

Query: 610 FLCGQGYSTSMVRRITGDNSACTSGNIGRVWDLNYPSFAVSISGT-RTVNQYFTRTLTNV 669
           FLCG  Y++  ++ I+GD   C+  N     +LNYPS +  +SGT  T +  F RTLTNV
Sbjct: 610 FLCGMNYTSKTLKIISGDTVKCSKKNKILPRNLNYPSMSAKLSGTDSTFSVTFNRTLTNV 669

Query: 670 ARGGSTYRAKISAPQG--LSITVNPTALSFNGIGDRKSFRLTVGGT--VSQSISSASLEW 728
               STY++K+ A  G  LSI V P+ L F  + +++SF +TV G+   S+  SSA+L W
Sbjct: 670 GTPNSTYKSKVVAGHGSKLSIKVTPSVLYFKTVNEKQSFSVTVTGSDVDSEVPSSANLIW 726

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q395476.2e-30271.76Cucumisin OS=Cucumis melo OX=3656 PE=1 SV=1[more]
Q9STF71.6e-17246.77Subtilisin-like protease SBT4.6 OS=Arabidopsis thaliana OX=3702 GN=SBT4.6 PE=3 S... [more]
Q8L7D26.0e-17246.79Subtilisin-like protease SBT4.12 OS=Arabidopsis thaliana OX=3702 GN=SBT4.12 PE=2... [more]
Q9FIF88.7e-17145.97Subtilisin-like protease SBT4.3 OS=Arabidopsis thaliana OX=3702 GN=SBT4.3 PE=3 S... [more]
Q9FIG28.7e-17145.95Subtilisin-like protease SBT4.13 OS=Arabidopsis thaliana OX=3702 GN=SBT4.13 PE=2... [more]
Match NameE-valueIdentityDescription
KAG6600967.10.0100.00hypothetical protein SDJN03_06200, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_022957348.10.097.55cucumisin-like [Cucurbita moschata][more]
XP_023527145.10.096.33cucumisin-like [Cucurbita pepo subsp. pepo][more]
XP_022957349.10.082.03cucumisin-like [Cucurbita moschata][more]
BBK45496.10.077.11pre-pro-cucumisin like serine protease [Trichosanthes bracteata] >BBK45497.1 tri... [more]
Match NameE-valueIdentityDescription
A0A6J1GZY50.097.55cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458774 PE=3 SV=1[more]
A0A6J1H1P20.082.03cucumisin-like OS=Cucurbita moschata OX=3662 GN=LOC111458775 PE=3 SV=1[more]
A0A4P2YW590.077.11Pre-pro-cucumisin like serine protease OS=Trichosanthes bracteata OX=486425 PE=2... [more]
K7NBW10.074.76Cucumisin OS=Siraitia grosvenorii OX=190515 PE=2 SV=1[more]
A0A1S3CF860.071.76LOW QUALITY PROTEIN: cucumisin OS=Cucumis melo OX=3656 GN=LOC103500253 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT3G46850.11.1e-17346.77Subtilase family protein [more]
AT5G59090.14.3e-17346.79subtilase 4.12 [more]
AT5G59090.24.7e-17246.65subtilase 4.12 [more]
AT5G59120.16.2e-17245.95subtilase 4.13 [more]
AT5G59090.36.2e-17246.79subtilase 4.12 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 522..538
score: 46.63
coord: 198..211
score: 51.61
coord: 130..149
score: 32.79
NoneNo IPR availableGENE3D3.50.30.30coord: 329..463
e-value: 3.7E-185
score: 618.1
NoneNo IPR availableGENE3D2.60.40.2310coord: 601..730
e-value: 5.0E-42
score: 144.7
NoneNo IPR availablePANTHERPTHR10795:SF649PUTATIVE-RELATEDcoord: 22..728
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 113..584
score: 27.15037
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 336..456
e-value: 2.35539E-15
score: 71.2907
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 631..727
e-value: 1.4E-27
score: 95.8
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 132..597
e-value: 3.7E-185
score: 618.1
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 108..588
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 16..109
e-value: 3.7E-27
score: 96.6
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 33..109
e-value: 3.8E-14
score: 53.1
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 131..576
e-value: 2.2E-41
score: 142.0
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 22..728
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 107..559
e-value: 9.46534E-121
score: 362.301

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g027340.m01Csor.00g027340.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
cellular_component GO:0005576 extracellular region
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity