Csor.00g016010 (gene) Silver-seed gourd (wild; sororia) v1

Overview
NameCsor.00g016010
Typegene
OrganismCucurbita argyrosperma subsp. sororia (Silver-seed gourd (wild; sororia) v1)
Descriptioniron-sulfur assembly protein IscA-like 2, mitochondrial
LocationCsor_Chr13: 943841 .. 947555 (-)
RNA-Seq ExpressionCsor.00g016010
SyntenyCsor.00g016010
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideCDSinitialstart_codonintroninternalterminalstop_codon
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCCCTAGATTGCTAATTCAGCGGTTAACACCATTCTTGGTGGCACGTATTCGAGAGAATCAGAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCATCTTCTTTGTTGGCATCTCCATCTCCATCTTCATCTGGGCCTATTGATACCATTCAAATGACTGATAATTGCATTCGGGTATTCTATACATATAATGTTATCCTTTTTTGTACAAGTACCATATGGTATAACTTAGGAATTCTTCATCCATTTCACTGTCATCCTCTAGTTTTAGTTCTTGATTTTGGACTATTCTCTTATTGTGCACCATTTGGGACTATAGTTTCTGGGTTTAGGATGCTAATCCCTTATTGTCCTGTTTGGATTCTTGAAATTTGAGTAAAGCATTTGAATTTTGAGCATTTCTCTTGTTACCACTTCAGCCTATATTGGGGTTCATAGAACGGTTTTGATTTACTTTTTTGCTTACTCTTGACATACCTTGTAGAGAATGAAAGAATTGCAAGCGCCAAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGATGTTCTGGATTTCAATATGTTTTCGATTTGGATGACAAAACCAATCCTGATGATAGGTTCAACAATTATTATCCACTTAAAGACTGTCACTGGTTTGATTTTACTTCCTATTTTTTTTTTTTTTTTTTTTTACTCTTCTCTCTTTTCATATTTGCAGGGTGTATGAGAAGGAGGGAGTTCGATTGGTTGTCGATAATATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTCGAGGAGCTGATTCGTTCTGCTTTTGTTGTAAGTGAAACTTATCTCTCATTATTGCTGGCTCACCACTTTTTCTTAGATTTGTTTATTATTTTCATACCAGTGTGTAGACCATTGCAAATTATAGTTCTATGACATCTATTCAATCAATGGAGTCACGATAAAGAAATTGAAATACTGAAAAACCACTTCCATTAATGTCATACTTAGAATATCTATCATTTGACTGTTGCATCATGCCGTTTACAGAGTCTCTTTCCCTTCATAATTACTGTTTGGTGTCCAGAGTATTATTTACGTTGAGAAGTATACTGAGAGGTGCGAATCCTTCAGCCCTGGGCTGGGAAACACTCCATACGGTAGATCCCTACCTGAAAAGACACACCATGTCCACTCTCATCTCTCCTTCCCCATATGTATGCGCCTCGACTTCTAACTGTTGGAGTTTGGCATTCTCCATTTTACCTTTGAAAATATGTGAACTATAGGGGTATAACATAGAAATGAAAATTTACTGTGTGAACAAAGGAAGTAGATACCGTAGAATTTCTAGGAATTTTCTATAACAACTACTGCTAGCATATGTTGTCTTCTTTTTGCTTTCCCTTTCGGGCTTCCCCTCAAGGCTTTTAAAACGCGTCTGCTAGGGAGAGGTTCCACACCCTTATAAAGGGTGTTTCATTCTTATCCTCAACCGATGTAGGATCTCACAATCCACCTTCCTTCGGATCCCAGCGTCCTCGCTAGCACTCGTTCCTTTCTCCAATCTATGTGAGATCCCCAATCCACCTCCTTTGGGGCCTAGTGTCCTTGCTAACAAACGCCTCGTGTCCACCCCCCTTCGGGGCTCAGCCTCCTCGATGGCACATATGTCTAGCTCTGATACCATTTGTAACAACTCAAGCCCACCGTTAACAGATATTGTTCTCTTTAAGCTTTCCCTTTCAGGCTTCCCCTCGAGGTTTTTAAAACACGTCTACTAGACACCCTTCGGTGTTTCGTTCTTCTCCCCAACTGACGTGGGATCTCACATTTTTCCTCTAAGCATCATCTATAATCTATAAAAAATAAAGTGATGCACCCTTGAGCAGTAATACGCCCTACAAGCCACATTTTACCCAAAAGTTTCCTATGCAATCTTCGATATCCCATCATAATAGAATAAGTAGTATTGCAGAAGTTGAATTCTTGTATTACGTGCAGGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGGTCAAACAATAATCACCTCAAAGCTTCCTTCCTATAAGGCATAGAAGTAGTTATCAAAACCTGAAGGTATGATGAGTATGTTTATCACTTGGTTGCTTCTGATAATAAGCCCATTTTATATTTTCATGTACATCTTCCACTTCTTTATCATCTGAGAACTTCAATCATTATTGCAGCGTTAAGCAAAAAATAACCTTATTGATTGTAATCGTCCTTGATCTAAAACTTTCCTCTTGTTCTGAGGATGAGTCGGCAATAATTTATTACATTGTGAAAATAGAAAATGTTCTGGATATTGGATTCATCTACTGGAAAAATCATTCCCAATTTTGTTATCACTTATAATTGTCCTATGGCCATTAGTATCACTATATTTGGGGGATAAATGACTGTATATTCTTATTTTATTCAAGAATCTTAATTAAACCCATTGTTTCTTAGAAGCTTATTAATAGAGCATTTAGTTGCTAGCATCATAGCTTCAAATCTTATATGGGTAAAATTTGGAGCAATCCTTTTACTCCAAAAGCATCAAAATGTATTTTTACCGTTACCTTCATTAAAGGTTTGTCAAAATATTGTAATTGTTAAGAATCCCGATCCCACATTTATCTCTATTACAAGGTATGTTAATACGATGGATGTTGAATTTCTACTATTGAACAATCAATTTAGATTTCTAATGATAGGAGGTGCGGGAGCTTGTCGTTGACAATTGAATTTCTATCATTTCTTCCGTGTACTCTTCCTAGGCATTGACTTGAGTATTTGAGTCATTAAGCAAATATTGACATTGGTATGTTTTTTTTTTCTATACATTAACAATGATTTTTTTTGCAAGGACAAAGAAATTTATTAGGTCCTCTGATATGAAAAGAACGTGCCCTAAGGTCTGAATTTATGGACAACAAAGGTGCAAGCCATGATGGCAAAGCACATGCTGCAAAACAAAATAGTATCACATAATGAAGTTACATTAGAACTTTCACTTTCTCATTTGGTAAAAATGGGAACTTTATGGTAAGCCCGTGATATAAATATTGATATTATATCATGAAAATGATTTATATTATCATAAAAGATGATATCATTTCAAGCAATATACGCTGTTCTAGGTTAATTTTGAATATTATAAACATATAATGAATACTTAGAAGATATTGTGAGATTATTTGGAATACCTGGTCAATATGCCATTATTATTACATGTTGTATGGACCTTCAAATGGCATGCCTTTGCTTCTCACAATTAGATTAGCAAAAAAGTGACCAAATTTCAATGCCTAATTGATTTACTAAACTATATTCTATTCTCTCATGTAGTGAAATGATTATTTTAAAGCATTCTCGAGCCTTTTTTCTTGCAAGCTTATTAGACATCAAATCATAAACTTAGCTCTTGTTTGATGGCTGGTCTAAGACACAAAATCAAATGCTTAACTCGTGTCTTTTGTAAGTGCTTTTTCTTCTACAGCTTCTGTCGCACATTGTGATGGCGTCGGTGCATGTGTTTATAGAATACACCGGAGCTGAGTTTAACGACGTCAAAATTTGTGAGGTTCCCATTAACCCAAATGTAGAAGAGTTTCACTTTCTTTCATCGTTTTCTATTGACTTTGATAGCTACCGTTCTTTCACTATTGGAGTTTCCAACGTTTCATGA

mRNA sequence

ATGGCCCCTAGATTGCTAATTCAGCGGTTAACACCATTCTTGGTGGCACGTATTCGAGAGAATCAGAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCATCTTCTTTGTTGGCATCTCCATCTCCATCTTCATCTGGGCCTATTGATACCATTCAAATGACTGATAATTGCATTCGGAGAATGAAAGAATTGCAAGCGCCAAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGATGTTCTGGATTTCAATATGTTTTCGATTTGGATGACAAAACCAATCCTGATGATAGGGTGTATGAGAAGGAGGGAGTTCGATTGGTTGTCGATAATATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTCGAGGAGCTGATTCGTTCTGCTTTTGTTGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGCTTCTGTCGCACATTGTGATGGCGTCGGTGCATGTGTTTATAGAATACACCGGAGCTGAGTTTAACGACGTCAAAATTTGTGAGGTTCCCATTAACCCAAATGTAGAAGAGTTTCACTTTCTTTCATCGTTTTCTATTGACTTTGATAGCTACCGTTCTTTCACTATTGGAGTTTCCAACGTTTCATGA

Coding sequence (CDS)

ATGGCCCCTAGATTGCTAATTCAGCGGTTAACACCATTCTTGGTGGCACGTATTCGAGAGAATCAGAGGCTTCTTAGCTCTTCTGTTTCAGCTCATCAAGAAGCATCTTCTTTGTTGGCATCTCCATCTCCATCTTCATCTGGGCCTATTGATACCATTCAAATGACTGATAATTGCATTCGGAGAATGAAAGAATTGCAAGCGCCAAAGGAAGAAAAGATGCTTCGCTTGAGTGTAGAAACTGGGGGATGTTCTGGATTTCAATATGTTTTCGATTTGGATGACAAAACCAATCCTGATGATAGGGTGTATGAGAAGGAGGGAGTTCGATTGGTTGTCGATAATATCTCATACGATTTTGTAAAAGGAGCAACAATTGATTATGTCGAGGAGCTGATTCGTTCTGCTTTTGTTGTGAGTACAAATCCAAGTGCCGTTGGTGGGTGTAGTTGTAAAAGTTCTTTCATGCTTCTGTCGCACATTGTGATGGCGTCGGTGCATGTGTTTATAGAATACACCGGAGCTGAGTTTAACGACGTCAAAATTTGTGAGGTTCCCATTAACCCAAATGTAGAAGAGTTTCACTTTCTTTCATCGTTTTCTATTGACTTTGATAGCTACCGTTCTTTCACTATTGGAGTTTCCAACGTTTCATGA

Protein sequence

MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCIRRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMLLSHIVMASVHVFIEYTGAEFNDVKICEVPINPNVEEFHFLSSFSIDFDSYRSFTIGVSNVS
Homology
BLAST of Csor.00g016010 vs. ExPASy Swiss-Prot
Match: Q8LCY2 (Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At5g03905 PE=1 SV=2)

HSP 1 Score: 204.1 bits (518), Expect = 1.6e-51
Identity = 108/156 (69.23%), Postives = 130/156 (83.33%), Query Frame = 0

Query: 4   RLLIQRLTPFLVARIRENQRLLS-SSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCIRR 63
           R L++R+ P+L  RIREN RLL+ SS SA +EASS  +S   SSS   D + ++DNCIRR
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSN--DVVHLSDNCIRR 62

Query: 64  MKELQAPK-EEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDFV 123
           MKELQ+ + E+KMLRL VETGGCSGFQY F+LD++TNPDDRV+EK GV+LVVDN+SYD V
Sbjct: 63  MKELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLV 122

Query: 124 KGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 158
           KGATIDY EELIR+AFVV+ NPSAVGGCSCKSSFM+
Sbjct: 123 KGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMV 156

BLAST of Csor.00g016010 vs. ExPASy Swiss-Prot
Match: Q86U28 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN=ISCA2 PE=1 SV=2)

HSP 1 Score: 119.8 bits (299), Expect = 3.9e-26
Identity = 70/137 (51.09%), Postives = 91/137 (66.42%), Query Frame = 0

Query: 23  RLLSSSV--SAHQEASSLLASPSPSSSGPIDTIQMTDNCIRRMKELQAPKEEKMLRLSVE 82
           RLL++S+   A +EASS  +SP          I++TD+C++R+ E+    E   LRL VE
Sbjct: 24  RLLTASLGPQARREASS--SSPEAGEG----QIRLTDSCVQRLLEITEGSE--FLRLQVE 83

Query: 83  TGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDFVKGATIDYVEELIRSAFVVS 142
            GGCSGFQY F LD   NPDDRV+E+ G R+VVD+ S  FVKGA +D+ +ELIRS+F V 
Sbjct: 84  GGGCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVL 143

Query: 143 TNPSAVGGCSCKSSFML 158
            NP A  GCSC SSF +
Sbjct: 144 NNPQAQQGCSCGSSFSI 152

BLAST of Csor.00g016010 vs. ExPASy Swiss-Prot
Match: Q5R788 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 3.9e-26
Identity = 70/137 (51.09%), Postives = 91/137 (66.42%), Query Frame = 0

Query: 23  RLLSSSV--SAHQEASSLLASPSPSSSGPIDTIQMTDNCIRRMKELQAPKEEKMLRLSVE 82
           RLL++S+   A +EASS  +SP          I++TD+C++R+ E+    E   LRL VE
Sbjct: 24  RLLAASLGPQARREASS--SSPEAGEG----QIRLTDSCVQRLLEITEGSE--FLRLQVE 83

Query: 83  TGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDFVKGATIDYVEELIRSAFVVS 142
            GGCSGFQY F LD   NPDDRV+E+ G R+VVD+ S  FVKGA +D+ +ELIRS+F V 
Sbjct: 84  GGGCSGFQYKFSLDTVINPDDRVFEQGGARVVVDSDSLAFVKGAQVDFSQELIRSSFQVL 143

Query: 143 TNPSAVGGCSCKSSFML 158
            NP A  GCSC SSF +
Sbjct: 144 NNPQAQQGCSCGSSFSI 152

BLAST of Csor.00g016010 vs. ExPASy Swiss-Prot
Match: Q9DCB8 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 GN=Isca2 PE=1 SV=2)

HSP 1 Score: 119.0 bits (297), Expect = 6.6e-26
Identity = 59/103 (57.28%), Postives = 74/103 (71.84%), Query Frame = 0

Query: 53  IQMTDNCIRRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLV 112
           I++TD+C++R+ E+    E   LRL VE GGCSGFQY F LD   NPDDRV+E+ G R+V
Sbjct: 50  IRLTDSCVQRLLEITEGSE--FLRLQVEGGGCSGFQYKFSLDTVINPDDRVFEQGGARVV 109

Query: 113 VDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSF 156
           VD+ S  FVKGA +D+ +ELIRS+F V  NP A  GCSC SSF
Sbjct: 110 VDSDSLAFVKGAQVDFSQELIRSSFQVLNNPQAQQGCSCGSSF 150

BLAST of Csor.00g016010 vs. ExPASy Swiss-Prot
Match: Q2TBG7 (Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=ISCA2 PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.9e-25
Identity = 66/136 (48.53%), Postives = 83/136 (61.03%), Query Frame = 0

Query: 20  ENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCIRRMKELQAPKEEKMLRLSV 79
           +  RLL++S           +SP          I +TD+C++R+ E+    E   LRL V
Sbjct: 19  QRSRLLAASRGPRALREVSFSSPEAGEG----QIHLTDSCVQRLLEITEGSE--FLRLEV 78

Query: 80  ETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDFVKGATIDYVEELIRSAFVV 139
           E GGCSGFQY F LD   NPDDRV+EK G R+VVD+ S  FVKGA +D+ +ELIRS+F V
Sbjct: 79  EGGGCSGFQYKFSLDTVINPDDRVFEKGGARVVVDSDSLAFVKGAHVDFSQELIRSSFQV 138

Query: 140 STNPSAVGGCSCKSSF 156
             NP A  GCSC SSF
Sbjct: 139 LNNPQAQQGCSCGSSF 148

BLAST of Csor.00g016010 vs. NCBI nr
Match: KAG6583457.1 (Iron-sulfur assembly protein IscA-like 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 421 bits (1082), Expect = 3.00e-148
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMLLSHIVMASVHVFIEYTGAEFNDV 180
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMLLSHIVMASVHVFIEYTGAEFNDV
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMLLSHIVMASVHVFIEYTGAEFNDV 180

Query: 181 KICEVPINPNVEEFHFLSSFSIDFDSYRSFTIGVSNVS 218
           KICEVPINPNVEEFHFLSSFSIDFDSYRSFTIGVSNVS
Sbjct: 181 KICEVPINPNVEEFHFLSSFSIDFDSYRSFTIGVSNVS 218

BLAST of Csor.00g016010 vs. NCBI nr
Match: XP_022964844.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata] >XP_023520276.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 301 bits (770), Expect = 1.33e-101
Identity = 156/157 (99.36%), Postives = 157/157 (100.00%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMV 157

BLAST of Csor.00g016010 vs. NCBI nr
Match: XP_022970473.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 298 bits (762), Expect = 2.20e-100
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           MA RLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI
Sbjct: 1   MASRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMV 157

BLAST of Csor.00g016010 vs. NCBI nr
Match: XP_038893859.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_038893860.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_038893861.1 iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida])

HSP 1 Score: 268 bits (684), Expect = 1.55e-88
Identity = 139/157 (88.54%), Postives = 147/157 (93.63%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           MA R LIQR+TP+LVARIRENQRLLSSSVSAHQEASS L  PSPS S  +DTI +TDNCI
Sbjct: 1   MASRSLIQRVTPYLVARIRENQRLLSSSVSAHQEASSSLTCPSPSPS--VDTIHLTDNCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           RRMKELQ PKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGV+L+VDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVKLIVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDYVEELIRSAF+VSTNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYVEELIRSAFIVSTNPSAVGGCSCKSSFMV 155

BLAST of Csor.00g016010 vs. NCBI nr
Match: XP_022152826.1 (iron-sulfur assembly protein IscA-like 2, mitochondrial [Momordica charantia])

HSP 1 Score: 266 bits (681), Expect = 4.74e-88
Identity = 137/157 (87.26%), Postives = 145/157 (92.36%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           M PRLLIQRLTP  +ARIRENQRLLSSSVSAHQE  SLL SPSPSSS  IDTI MTDNCI
Sbjct: 1   MVPRLLIQRLTPHFIARIRENQRLLSSSVSAHQEVPSLLTSPSPSSSVLIDTIHMTDNCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           ++MKELQ P EEKMLRLSVETGGCSGFQYVF+LDDKTNPDDRVYEK+GV+LVVDNISYDF
Sbjct: 61  QKMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDKTNPDDRVYEKDGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDY EELIRSAFVV+TNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYAEELIRSAFVVTTNPSAVGGCSCKSSFMV 157

BLAST of Csor.00g016010 vs. ExPASy TrEMBL
Match: A0A6J1HPD1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111464825 PE=4 SV=1)

HSP 1 Score: 301 bits (770), Expect = 6.43e-102
Identity = 156/157 (99.36%), Postives = 157/157 (100.00%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI
Sbjct: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMV 157

BLAST of Csor.00g016010 vs. ExPASy TrEMBL
Match: A0A6J1I3Y1 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111469443 PE=4 SV=1)

HSP 1 Score: 298 bits (762), Expect = 1.06e-100
Identity = 155/157 (98.73%), Postives = 156/157 (99.36%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           MA RLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI
Sbjct: 1   MASRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF
Sbjct: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMV 157

BLAST of Csor.00g016010 vs. ExPASy TrEMBL
Match: A0A6J1DIX6 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111020448 PE=4 SV=1)

HSP 1 Score: 266 bits (681), Expect = 2.29e-88
Identity = 137/157 (87.26%), Postives = 145/157 (92.36%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           M PRLLIQRLTP  +ARIRENQRLLSSSVSAHQE  SLL SPSPSSS  IDTI MTDNCI
Sbjct: 1   MVPRLLIQRLTPHFIARIRENQRLLSSSVSAHQEVPSLLTSPSPSSSVLIDTIHMTDNCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           ++MKELQ P EEKMLRLSVETGGCSGFQYVF+LDDKTNPDDRVYEK+GV+LVVDNISYDF
Sbjct: 61  QKMKELQEPMEEKMLRLSVETGGCSGFQYVFNLDDKTNPDDRVYEKDGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDY EELIRSAFVV+TNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYAEELIRSAFVVTTNPSAVGGCSCKSSFMV 157

BLAST of Csor.00g016010 vs. ExPASy TrEMBL
Match: A0A1S3C5P3 (iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103497193 PE=4 SV=1)

HSP 1 Score: 266 bits (679), Expect = 4.63e-88
Identity = 135/157 (85.99%), Postives = 147/157 (93.63%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           M PR LIQR+TP+LVARI+ENQRLLSSS +A+QEASSL +SPSPS S P+DTI MTD+CI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSATAYQEASSLSSSPSPSPSAPVDTIHMTDSCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           RRMKELQ PKEEKMLRLSVETGGCSGFQYVF+LD KT PDDR+YEKEGV+LVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTTPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMV 157

BLAST of Csor.00g016010 vs. ExPASy TrEMBL
Match: A0A0A0LXX8 (Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G496290 PE=4 SV=1)

HSP 1 Score: 261 bits (666), Expect = 4.11e-86
Identity = 135/157 (85.99%), Postives = 145/157 (92.36%), Query Frame = 0

Query: 1   MAPRLLIQRLTPFLVARIRENQRLLSSSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCI 60
           M PR LIQR+TP+LVARI+ENQRLLSSS SA+QEA  L  SPSPS S P+DTI MTD+CI
Sbjct: 1   MGPRSLIQRVTPYLVARIKENQRLLSSSASAYQEA--LPLSPSPSPSAPVDTIHMTDSCI 60

Query: 61  RRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDF 120
           RRMKELQ PKEEKMLRLSVETGGCSGFQYVF+LD KTNPDDR+YEKEGV+LVVDNISYDF
Sbjct: 61  RRMKELQDPKEEKMLRLSVETGGCSGFQYVFNLDGKTNPDDRIYEKEGVKLVVDNISYDF 120

Query: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 157
           VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFM+
Sbjct: 121 VKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFMV 155

BLAST of Csor.00g016010 vs. TAIR 10
Match: AT5G03905.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 204.1 bits (518), Expect = 1.1e-52
Identity = 108/156 (69.23%), Postives = 130/156 (83.33%), Query Frame = 0

Query: 4   RLLIQRLTPFLVARIRENQRLLS-SSVSAHQEASSLLASPSPSSSGPIDTIQMTDNCIRR 63
           R L++R+ P+L  RIREN RLL+ SS SA +EASS  +S   SSS   D + ++DNCIRR
Sbjct: 3   RSLVKRVAPYLAGRIRENHRLLNFSSASAIKEASSSSSSQPESSSN--DVVHLSDNCIRR 62

Query: 64  MKELQAPK-EEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLVVDNISYDFV 123
           MKELQ+ + E+KMLRL VETGGCSGFQY F+LD++TNPDDRV+EK GV+LVVDN+SYD V
Sbjct: 63  MKELQSSEPEKKMLRLGVETGGCSGFQYKFELDNRTNPDDRVFEKNGVKLVVDNVSYDLV 122

Query: 124 KGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSFML 158
           KGATIDY EELIR+AFVV+ NPSAVGGCSCKSSFM+
Sbjct: 123 KGATIDYEEELIRAAFVVAVNPSAVGGCSCKSSFMV 156

BLAST of Csor.00g016010 vs. TAIR 10
Match: AT2G16710.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 70.9 bits (172), Expect = 1.5e-12
Identity = 42/129 (32.56%), Postives = 71/129 (55.04%), Query Frame = 0

Query: 34  EASSLLASPSPSSSGPI---DTIQMTDNCIRRMKELQAPKEEKMLRLSVETGGCSGFQYV 93
           +AS +LA+ + +  GP      + +TD    R+  L   +++  LRL V+  GC+G  Y 
Sbjct: 2   KASQILAA-AAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYT 61

Query: 94  FDLDDKTNPDDRVYEKEGVRLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCS 153
            +  D+    D + E++GVR++V+  +   V G  +D+V++ +RS FV   NP++ G C 
Sbjct: 62  LNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPNSQGQCG 121

Query: 154 CKSSFMLLS 160
           C  SFM  S
Sbjct: 122 CGESFMTTS 128

BLAST of Csor.00g016010 vs. TAIR 10
Match: AT2G16710.3 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 67.0 bits (162), Expect = 2.1e-11
Identity = 43/135 (31.85%), Postives = 73/135 (54.07%), Query Frame = 0

Query: 34  EASSLLASPSPSSSGPI---DTIQMTDNCIRRMKELQAPKEEKMLRLSVETGGCSGFQYV 93
           +AS +LA+ + +  GP      + +TD    R+  L   +++  LRL V+  GC+G  Y 
Sbjct: 2   KASQILAA-AAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYT 61

Query: 94  FDLDDKTNPD------DRVYEKEGVRLVVDNISYDFVKGATIDYVEELIRSAFVVSTNPS 153
            +   KT+ +      D + E++GVR++V+  +   V G  +D+V++ +RS FV   NP+
Sbjct: 62  LNYAGKTSGNDEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLRSEFVF-INPN 121

Query: 154 AVGGCSCKSSFMLLS 160
           + G C C  SFM  S
Sbjct: 122 SQGQCGCGESFMTTS 134

BLAST of Csor.00g016010 vs. TAIR 10
Match: AT2G36260.1 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 66.2 bits (160), Expect = 3.6e-11
Identity = 35/103 (33.98%), Postives = 60/103 (58.25%), Query Frame = 0

Query: 53  IQMTDNCIRRMKELQAPKEEKMLRLSVETGGCSGFQYVFDLDDKTNPDDRVYEKEGVRLV 112
           + ++D    R+++L   +++  LRL+VE  GC+G  YV +   +    D V E++GV+++
Sbjct: 6   LALSDTAAARIRQLLQHQQKPFLRLAVEAKGCNGLSYVLNYAQEKGKFDEVVEEKGVKIL 65

Query: 113 VDNISYDFVKGATIDYVEELIRSAFVVSTNPSAVGGCSCKSSF 156
           VD  +   V G  +D+V++ +RS FV   NP+A   C C  SF
Sbjct: 66  VDPKAVMHVIGTEMDFVDDKLRSEFVF-VNPNAT-KCGCGESF 106

BLAST of Csor.00g016010 vs. TAIR 10
Match: AT2G16710.2 (Iron-sulphur cluster biosynthesis family protein )

HSP 1 Score: 52.8 bits (125), Expect = 4.1e-07
Identity = 30/104 (28.85%), Postives = 57/104 (54.81%), Query Frame = 0

Query: 34  EASSLLASPSPSSSGPI---DTIQMTDNCIRRMKELQAPKEEKMLRLSVETGGCSGFQYV 93
           +AS +LA+ + +  GP      + +TD    R+  L   +++  LRL V+  GC+G  Y 
Sbjct: 2   KASQILAA-AAARVGPALRKQVLTLTDEAASRVHHLLQQRQKPFLRLGVKARGCNGLSYT 61

Query: 94  FDLDDKTNPDDRVYEKEGVRLVVDNISYDFVKGATIDYVEELIR 135
            +  D+    D + E++GVR++V+  +   V G  +D+V++ +R
Sbjct: 62  LNYADEKGKFDELVEEKGVRILVEPKALMHVIGTKMDFVDDKLR 104

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LCY21.6e-5169.23Iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Arabidopsis thaliana ... [more]
Q86U283.9e-2651.09Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Homo sapiens OX=9606 GN... [more]
Q5R7883.9e-2651.09Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Pongo abelii OX=9601 GN... [more]
Q9DCB86.6e-2657.28Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Mus musculus OX=10090 G... [more]
Q2TBG71.9e-2548.53Iron-sulfur cluster assembly 2 homolog, mitochondrial OS=Bos taurus OX=9913 GN=I... [more]
Match NameE-valueIdentityDescription
KAG6583457.13.00e-148100.00Iron-sulfur assembly protein IscA-like 2, mitochondrial, partial [Cucurbita argy... [more]
XP_022964844.11.33e-10199.36iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita moschata] >XP... [more]
XP_022970473.12.20e-10098.73iron-sulfur assembly protein IscA-like 2, mitochondrial [Cucurbita maxima][more]
XP_038893859.11.55e-8888.54iron-sulfur assembly protein IscA-like 2, mitochondrial [Benincasa hispida] >XP_... [more]
XP_022152826.14.74e-8887.26iron-sulfur assembly protein IscA-like 2, mitochondrial [Momordica charantia][more]
Match NameE-valueIdentityDescription
A0A6J1HPD16.43e-10299.36iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita moschata OX... [more]
A0A6J1I3Y11.06e-10098.73iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucurbita maxima OX=3... [more]
A0A6J1DIX62.29e-8887.26iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Momordica charantia O... [more]
A0A1S3C5P34.63e-8885.99iron-sulfur assembly protein IscA-like 2, mitochondrial OS=Cucumis melo OX=3656 ... [more]
A0A0A0LXX84.11e-8685.99Fe-S_biosyn domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G496290... [more]
Match NameE-valueIdentityDescription
AT5G03905.11.1e-5269.23Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.11.5e-1232.56Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.32.1e-1131.85Iron-sulphur cluster biosynthesis family protein [more]
AT2G36260.13.6e-1133.98Iron-sulphur cluster biosynthesis family protein [more]
AT2G16710.24.1e-0728.85Iron-sulphur cluster biosynthesis family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (sororia) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000361FeS cluster biogenesisPFAMPF01521Fe-S_biosyncoord: 53..152
e-value: 1.1E-15
score: 57.8
IPR035903HesB-like domain superfamilyGENE3D2.60.300.12coord: 52..159
e-value: 6.0E-34
score: 118.4
IPR035903HesB-like domain superfamilySUPERFAMILY89360HesB-like domaincoord: 52..145
IPR016092FeS cluster insertion proteinTIGRFAMTIGR00049TIGR00049coord: 53..156
e-value: 1.5E-33
score: 113.2
NoneNo IPR availablePANTHERPTHR43011:SF4BNACNNG10620D PROTEINcoord: 4..157
NoneNo IPR availablePANTHERPTHR43011IRON-SULFUR CLUSTER ASSEMBLY 2 HOMOLOG, MITOCHONDRIALcoord: 4..157

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Csor.00g016010.m01Csor.00g016010.m01mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016226 iron-sulfur cluster assembly
biological_process GO:0106035 protein maturation by [4Fe-4S] cluster transfer
cellular_component GO:0005739 mitochondrion
molecular_function GO:0051537 2 iron, 2 sulfur cluster binding
molecular_function GO:0051539 4 iron, 4 sulfur cluster binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0051536 iron-sulfur cluster binding