CsaV3_6G007560 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_6G007560 vs. NCBI nr
Match: XP_004140662.1 (cytochrome P450 81Q32 [Cucumis sativus] >AIY67847.1 cytochrome P450 [Cucumis sativus] >KGN46390.1 hypothetical protein Csa_005013 [Cucumis sativus])

HSP 1 Score: 1011.1 bits (2613), Expect = 3.3e-291
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0

Query: 1   MEMEAITYLPFLITFLALVSIFFIQKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLSQ 60
           MEMEAITYLPFLITFLALVSIFFIQKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLSQ
Sbjct: 1   MEMEAITYLPFLITFLALVSIFFIQKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLSQ 60

Query: 61  KYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYG 120
           KYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYG
Sbjct: 61  KYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYG 120

Query: 121 DHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTF 180
           DHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTF
Sbjct: 121 DHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTF 180

Query: 181 NIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVES 240
           NIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVES
Sbjct: 181 NIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVES 240

Query: 241 LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 300
           LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA
Sbjct: 241 LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 300

Query: 301 TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL 360
           TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL
Sbjct: 301 TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL 360

Query: 361 YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG 420
           YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG
Sbjct: 361 YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG 420

Query: 421 REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQ 480
           REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQ
Sbjct: 421 REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQ 480

Query: 481 GISMPKVEPLEALCRARPIIKKLALDD 508
           GISMPKVEPLEALCRARPIIKKLALDD
Sbjct: 481 GISMPKVEPLEALCRARPIIKKLALDD 507

BLAST of CsaV3_6G007560 vs. NCBI nr
Match: KAA0039876.1 (cytochrome P450 81E8-like [Cucumis melo var. makuwa] >TYK24625.1 cytochrome P450 81E8-like [Cucumis melo var. makuwa])

HSP 1 Score: 932.2 bits (2408), Expect = 1.9e-267
Identity = 474/509 (93.12%), Postives = 484/509 (95.09%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTK-----KNLPPSPLALPILGHLHLLKHPIHRTLHN 62
           ME   Y PFLIT LAL+SIFFI+K+K      NLPPSPL LPILGHLHLLKHPIHRTLH 
Sbjct: 1   METNAYFPFLITLLALISIFFIRKSKTKTKRNNLPPSPLPLPILGHLHLLKHPIHRTLHT 60

Query: 63  LSQKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 122
           LSQKYGPIFTLRLGSRLVVVVSS SA EECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA
Sbjct: 61  LSQKYGPIFTLRLGSRLVVVVSSTSAAEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 120

Query: 123 PYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSE 182
           PYGDHWRNLRRLSATE+LSTIRLNMSARIRKEEI ILIKKLNRV  T+FGKVKLKSLFSE
Sbjct: 121 PYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRVSSTDFGKVKLKSLFSE 180

Query: 183 LTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR 242
           LTFN+IMRM+AGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR
Sbjct: 181 LTFNMIMRMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR 240

Query: 243 VESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTIL 302
           VESLGKDTDWFLQNLVDEHR NEEEGEKGNTM+SHLL LQKTQPDYYTDEIIKGLIVTIL
Sbjct: 241 VESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQPDYYTDEIIKGLIVTIL 300

Query: 303 SAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISET 362
           SAATETSSVTIEWAMSNLLNHPEALKKVK+ELD QIGQDRILDEEDISNLPYLQNVISET
Sbjct: 301 SAATETSSVTIEWAMSNLLNHPEALKKVKEELDTQIGQDRILDEEDISNLPYLQNVISET 360

Query: 363 LRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESD 422
           LRLYPPAPLLAPHLSSSSCSLGGYHIPADT+LMVNAWAIQRDPKVWEDSTSFKPERFESD
Sbjct: 361 LRLYPPAPLLAPHLSSSSCSLGGYHIPADTILMVNAWAIQRDPKVWEDSTSFKPERFESD 420

Query: 423 HQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT 482
            Q REGS NNNNGY FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT
Sbjct: 421 -QVREGS-NNNNGYRFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT 480

Query: 483 EGQGISMPKVEPLEALCRARPIIKKLALD 507
           EGQGISMPKVEPLEALCRARPIIKKLALD
Sbjct: 481 EGQGISMPKVEPLEALCRARPIIKKLALD 507

BLAST of CsaV3_6G007560 vs. NCBI nr
Match: XP_008459939.1 (PREDICTED: cytochrome P450 81E8-like [Cucumis melo])

HSP 1 Score: 929.9 bits (2402), Expect = 9.6e-267
Identity = 473/509 (92.93%), Postives = 483/509 (94.89%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTK-----KNLPPSPLALPILGHLHLLKHPIHRTLHN 62
           ME   Y PFLIT LAL+SIFFI+K+K      NLPPSPL LPILGHLHLLKHPIHRTLH 
Sbjct: 1   METNAYFPFLITLLALISIFFIRKSKTKTKRNNLPPSPLPLPILGHLHLLKHPIHRTLHT 60

Query: 63  LSQKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 122
           LSQKYGPIFTLRLGSRLVVVVSS SA EECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA
Sbjct: 61  LSQKYGPIFTLRLGSRLVVVVSSTSAAEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 120

Query: 123 PYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSE 182
           PYGDHWRNLRRLSATE+LSTIRLNMSARIRKEEI ILIKKLNRV  T+FGKVKLKSLFSE
Sbjct: 121 PYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRVSSTDFGKVKLKSLFSE 180

Query: 183 LTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR 242
           LTFN+IM M+AGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR
Sbjct: 181 LTFNMIMTMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR 240

Query: 243 VESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTIL 302
           VESLGKDTDWFLQNLVDEHR NEEEGEKGNTM+SHLL LQKTQPDYYTDEIIKGLIVTIL
Sbjct: 241 VESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQPDYYTDEIIKGLIVTIL 300

Query: 303 SAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISET 362
           SAATETSSVTIEWAMSNLLNHPEALKKVK+ELD QIGQDRILDEEDISNLPYLQNVISET
Sbjct: 301 SAATETSSVTIEWAMSNLLNHPEALKKVKEELDTQIGQDRILDEEDISNLPYLQNVISET 360

Query: 363 LRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESD 422
           LRLYPPAPLLAPHLSSSSCSLGGYHIPADT+LMVNAWAIQRDPKVWEDSTSFKPERFESD
Sbjct: 361 LRLYPPAPLLAPHLSSSSCSLGGYHIPADTILMVNAWAIQRDPKVWEDSTSFKPERFESD 420

Query: 423 HQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT 482
            Q REGS NNNNGY FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT
Sbjct: 421 -QVREGS-NNNNGYRFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT 480

Query: 483 EGQGISMPKVEPLEALCRARPIIKKLALD 507
           EGQGISMPKVEPLEALCRARPIIKKLALD
Sbjct: 481 EGQGISMPKVEPLEALCRARPIIKKLALD 507

BLAST of CsaV3_6G007560 vs. NCBI nr
Match: XP_038907082.1 (cytochrome P450 81Q32-like [Benincasa hispida])

HSP 1 Score: 872.8 bits (2254), Expect = 1.4e-249
Identity = 434/508 (85.43%), Postives = 468/508 (92.13%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQK----TKKNLPPSPLALPILGHLHLLKHPIHRTLHNL 62
           ME  TY P LITFLAL+SIF  Q     TK NLPPSPLALPILGHLHLLKHPIHRTLH+L
Sbjct: 1   METTTYFPVLITFLALISIFLFQNRKHTTKTNLPPSPLALPILGHLHLLKHPIHRTLHSL 60

Query: 63  SQKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAP 122
           +Q +GPIFTLRLGSR VVVVSS SA+EECFTTNDIVFANRP+FVSGKYL+YGN+TLGAAP
Sbjct: 61  AQNHGPIFTLRLGSRPVVVVSSTSAVEECFTTNDIVFANRPQFVSGKYLSYGNTTLGAAP 120

Query: 123 YGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSEL 182
           YGDHWRNLRRLSATE+LS+IRLNMSA IRKEEI IL++KL +V GTE+GKVKLK+ FSEL
Sbjct: 121 YGDHWRNLRRLSATEVLSSIRLNMSAGIRKEEIKILLRKLYKVSGTEYGKVKLKTPFSEL 180

Query: 183 TFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRV 242
           TFN+IMRM+AGKRYYGEEVSELEEAKKFREIM+++FQLGSYPGDFLPFLKWVDWQY KR+
Sbjct: 181 TFNMIMRMVAGKRYYGEEVSELEEAKKFREIMQKSFQLGSYPGDFLPFLKWVDWQYAKRL 240

Query: 243 ESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILS 302
           E+LGKD D FLQNLVDEHRRN+ +G++GNTMISH+LCLQKTQPDYYTDEIIKGLIVTILS
Sbjct: 241 ENLGKDMDLFLQNLVDEHRRNQGDGKQGNTMISHILCLQKTQPDYYTDEIIKGLIVTILS 300

Query: 303 AATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETL 362
           AATETSS TIEWAMSNLLNHPE L K K+ELDKQIGQDR+LDEEDI NLPYLQNVISETL
Sbjct: 301 AATETSSATIEWAMSNLLNHPEVLNKAKNELDKQIGQDRLLDEEDICNLPYLQNVISETL 360

Query: 363 RLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDH 422
           RLYPPAPLLAPH SSSSC LGGYHIPA+TMLMVNAW IQRDPKVWEDSTSFKPERFES+ 
Sbjct: 361 RLYPPAPLLAPHFSSSSCCLGGYHIPANTMLMVNAWTIQRDPKVWEDSTSFKPERFESE- 420

Query: 423 QGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTE 482
               G ++NNNGY FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTE
Sbjct: 421 ---GGGSSNNNGYGFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTE 480

Query: 483 GQGISMPKVEPLEALCRARPIIKKLALD 507
           GQGISMPKVEPLEALCRARPI+K LALD
Sbjct: 481 GQGISMPKVEPLEALCRARPIMKNLALD 504

BLAST of CsaV3_6G007560 vs. NCBI nr
Match: KAG7014609.1 (Cytochrome P450 81E8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 781.9 bits (2018), Expect = 3.2e-222
Identity = 394/505 (78.02%), Postives = 442/505 (87.52%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTK---KNLPPSPLALPILGHLHLLKHPIHRTLHNLS 62
           ME  +Y+  LITFLA+ SIF +QK K   KNLPP+P ALPILGHLHLLKHP+HRTL +L+
Sbjct: 1   METSSYVAALITFLAVGSIFLLQKRKNRAKNLPPTPWALPILGHLHLLKHPVHRTLQSLA 60

Query: 63  QKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPY 122
           QK+GPIFTLRLGSRL VVVSS  A+EECFTTNDIVFANRP+FVSG YL+YGN+T+GAAPY
Sbjct: 61  QKHGPIFTLRLGSRLAVVVSSAPAVEECFTTNDIVFANRPQFVSGMYLSYGNTTMGAAPY 120

Query: 123 GDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELT 182
           GDHWRNLRRLS+TEILS+IRLNMSA IRKEE+ IL++KL +    +FGKVKLK+LFSELT
Sbjct: 121 GDHWRNLRRLSSTEILSSIRLNMSAGIRKEEVNILLRKLYQFSRHDFGKVKLKTLFSELT 180

Query: 183 FNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVE 242
           FNIIMRM+AGKRYYG+EV+ELEEAK+FR+IME +F+LGSYPGDFLPFLKWVD QY KR++
Sbjct: 181 FNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLPFLKWVDRQYPKRMK 240

Query: 243 SLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSA 302
           SLGK  D  LQNLVDEHRR + EG+K +TMISHLLCLQ++QP+YY+DEIIKGLIVTIL+A
Sbjct: 241 SLGKAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEIIKGLIVTILTA 300

Query: 303 ATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLR 362
           ATETSSVTIEWAMSNLLNHPEAL K + ELD QIGQDR+L+EEDI NLPYLQNVISETLR
Sbjct: 301 ATETSSVTIEWAMSNLLNHPEALDKARAELDAQIGQDRLLEEEDIPNLPYLQNVISETLR 360

Query: 363 LYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQ 422
           LYP APLLAPH SS SCSLGGYHIPA T L+VNAWA+QRDP+VWED T FKPERFES   
Sbjct: 361 LYPAAPLLAPHFSSESCSLGGYHIPAGTTLLVNAWAVQRDPEVWEDPTRFKPERFESGGV 420

Query: 423 GREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEG 482
                    N Y FLPFGLGRRACPGMGMANRVVGL LGSLIQSFEWKRVS++E+DMTE 
Sbjct: 421 AA-------NAYKFLPFGLGRRACPGMGMANRVVGLALGSLIQSFEWKRVSDEELDMTES 480

Query: 483 QGISMPKVEPLEALCRARPIIKKLA 505
           QGISM KVEPLEALCRARPIIK LA
Sbjct: 481 QGISMYKVEPLEALCRARPIIKNLA 498

BLAST of CsaV3_6G007560 vs. ExPASy Swiss-Prot
Match: Q6WNQ8 (Cytochrome P450 81E8 OS=Medicago truncatula OX=3880 GN=CYP81E8 PE=2 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 4.1e-151
Identity = 283/503 (56.26%), Postives = 357/503 (70.97%), Query Frame = 0

Query: 8   YLPFLITFLALV---SIFFIQKTK-KNLPPSPLALPILGHLHLLKHPIHRTLHNLSQKYG 67
           YL  +I+   L+    +FF    K KNLPP P  LPI+G+LH LK P+H T H LSQKYG
Sbjct: 5   YLSLIISLFFLIITLKVFFNTSRKFKNLPPGPQCLPIIGNLHQLKQPLHHTFHTLSQKYG 64

Query: 68  PIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHW 127
            IF+L  GSRLVVVVSS++  +ECFT NDIV ANRP F++GKY+ Y N+T+  +PYGDHW
Sbjct: 65  QIFSLWFGSRLVVVVSSLTIAQECFTKNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHW 124

Query: 128 RNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTFNII 187
           RNLRR+ + EILS+ RLN    IR++EI  LI+KL +     F +V+L+ +FSE+TFN I
Sbjct: 125 RNLRRILSIEILSSHRLNSFLEIRRDEIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTI 184

Query: 188 MRMLAGKRYYGE--EVSELEEAKKFREIMERAFQLG--SYPGDFLPFLKWVDWQ-YTKRV 247
           MRM++GKRYYG   +VS++EEA+ FR I++    LG  +  GDFL FL+W D+    KR+
Sbjct: 185 MRMVSGKRYYGNDCDVSDVEEARLFRGIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRL 244

Query: 248 ESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILS 307
           + + K TD FLQ L+DEHR  +      NTMI HLL  Q++QP+YYTD+IIKGL+V +L 
Sbjct: 245 KKISKRTDAFLQGLIDEHRFGK---RNSNTMIDHLLTQQQSQPEYYTDQIIKGLMVVMLL 304

Query: 308 AATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETL 367
           A T+TSSVTIEWAMSNLLNHPE +KK K+ELD  IG DR +DE DIS LPYLQ+++ ETL
Sbjct: 305 AGTDTSSVTIEWAMSNLLNHPEIMKKAKNELDTHIGHDRQVDEHDISKLPYLQSIVYETL 364

Query: 368 RLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDH 427
           RL+  APLL PHLSS   SLGGY+IP +T+LMVNAW I RDP +W D T FKPERFE   
Sbjct: 365 RLHAAAPLLVPHLSSEDFSLGGYNIPQNTILMVNAWVIHRDPNLWSDPTCFKPERFE--- 424

Query: 428 QGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTE 487
             +EG  N       L FGLGRRACPG  ++ R  GLTLG LIQ FEWKR+ E++IDM E
Sbjct: 425 --KEGEVN-----KLLSFGLGRRACPGENLSQRTEGLTLGLLIQCFEWKRIGEEKIDMVE 484

Query: 488 GQGISMPKVEPLEALCRARPIIK 502
            +GI+  K   L A+C+ R  +K
Sbjct: 485 AKGITAGKKTSLNAMCKVRHPLK 494

BLAST of CsaV3_6G007560 vs. ExPASy Swiss-Prot
Match: W8JMU7 (Cytochrome P450 81Q32 OS=Catharanthus roseus OX=4058 GN=CYP81Q32 PE=2 SV=1)

HSP 1 Score: 530.0 bits (1364), Expect = 2.9e-149
Identity = 265/508 (52.17%), Postives = 370/508 (72.83%), Query Frame = 0

Query: 1   MEMEAITYLPFLITFLAL-VSIFFIQKTKKNLPPSP-LALPILGHLHLLKHPIHRTLHNL 60
           ME   + Y    +  L++ + +F + + ++NLPPSP LALP++GHLHL+   +HR+L++L
Sbjct: 1   MESSTLLYTFLAVVLLSISLKLFPVSRRRRNLPPSPGLALPVIGHLHLIGKLLHRSLYDL 60

Query: 61  SQKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAP 120
           S+KYG +F+L+LG+RLV+VVSS +A EECFT NDIVFANRP F+ GKY+ Y  +T+  +P
Sbjct: 61  SKKYGSVFSLQLGNRLVLVVSSPAAAEECFTKNDIVFANRPLFILGKYIGYNYTTMVGSP 120

Query: 121 YGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSEL 180
           YG+HWRNLRRL+A EI S   LN    IR++E+  L+  L +  G +FGKV++KS  SEL
Sbjct: 121 YGEHWRNLRRLAAVEIFSAGSLNRFLSIREDEVKQLLLSLYQSSGQDFGKVEMKSKLSEL 180

Query: 181 TFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQL--GSYPGDFLPFLKWVDWQ-YT 240
           +FN+ MRM+AGKRY+G++V + +EAK FR ++   F+    S PGDF+PFL+W+D++ Y 
Sbjct: 181 SFNVTMRMVAGKRYFGQDV-DSDEAKLFRALIGEVFEHAGASNPGDFVPFLRWIDFKNYE 240

Query: 241 KRVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVT 300
           K+V  + ++ D FLQ L+ E R N    +   TMI HLL LQ++QP+YYTD+IIKG+I+ 
Sbjct: 241 KKVSKISQEMDAFLQRLIHESRIN----KNNVTMIDHLLSLQESQPEYYTDQIIKGIIMV 300

Query: 301 ILSAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVIS 360
           +L A T+TS+VT+EWAMS LLNHPE L+K + E++ Q+G +R+++E+D+  L YL N+IS
Sbjct: 301 LLLAGTDTSAVTVEWAMSLLLNHPETLEKARTEIETQVGSNRLIEEQDLPKLTYLHNIIS 360

Query: 361 ETLRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFE 420
           ET RL P AP+L PH SS  C + GY +P  T+L+VNAWAI RDP+ W++ T FKPER  
Sbjct: 361 ETFRLCPAAPMLVPHESSDDCKVQGYDVPKGTILLVNAWAIHRDPEFWDEPTLFKPER-- 420

Query: 421 SDHQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEID 480
             H G E   +       +PFG+GRR+CPG G+A RVVGLTLG+LIQ FEWKR+ E +ID
Sbjct: 421 --HGGVELEPS-----KLMPFGMGRRSCPGSGLAQRVVGLTLGALIQCFEWKRIGEAKID 480

Query: 481 MTEGQGISMPKVEPLEALCRARPIIKKL 504
           M EG G++MPK +PLEALC+ R I+ K+
Sbjct: 481 MAEGSGLTMPKAQPLEALCKPRNILHKV 494

BLAST of CsaV3_6G007560 vs. ExPASy Swiss-Prot
Match: Q6WNQ9 (Isoflavone 3'-hydroxylase (Fragment) OS=Medicago truncatula OX=3880 GN=CYP81E9 PE=1 SV=1)

HSP 1 Score: 521.5 bits (1342), Expect = 1.0e-146
Identity = 266/499 (53.31%), Postives = 356/499 (71.34%), Query Frame = 0

Query: 9   LPFLITFLALVSIFFIQKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLSQKYGPIFTL 68
           L F+IT   L+ I    +  KNLPP P  +PI+G+LH LKHP+HRT   LSQ YG IF+L
Sbjct: 10  LSFIITIKILLKI--TSRRLKNLPPGPPTIPIIGNLHHLKHPLHRTFTTLSQTYGDIFSL 69

Query: 69  RLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHWRNLRR 128
             GSRLVVVVSS S   ECFT NDI+ ANRP F++GKY+ Y  +TLG+A YGDHWRNLRR
Sbjct: 70  WFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNLRR 129

Query: 129 LSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTE---FGKVKLKSLFSELTFNIIMR 188
           ++  ++LS  RLN    +R++E   LI+KL + + +E   F KV+L+   +E+TFN +MR
Sbjct: 130 ITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAMMR 189

Query: 189 MLAGKRYYGE--EVSELEEAKKFREIMERAFQL--GSYPGDFLPFLKWVDW-QYTKRVES 248
           M++GKRYYG+  +VS++EEAK+FREI+     L   +  GDFLP L+ VD     KR + 
Sbjct: 190 MISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRCKR 249

Query: 249 LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 308
           + K ++ FL+ L++EHRR     + G TMI HLL L ++QP+YY+D +IKGLI  +L A 
Sbjct: 250 IAKRSNAFLEGLIEEHRRGNIHSD-GGTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAG 309

Query: 309 TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL 368
           T+TS+VTIEW MS LLNHPE LKK K+ELD QIG+++++DE+D+S LPYLQN+ISETLRL
Sbjct: 310 TDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRL 369

Query: 369 YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG 428
           +PPAPLL PH SS  C++G +++P DT+++ N W I RDPK W D+ SFKPERFE + + 
Sbjct: 370 HPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKEEEV 429

Query: 429 REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQ 488
            +           + FGLGRRACPG+ +A R VG T+G LIQ FEW+R SE+++DM EG+
Sbjct: 430 NK----------VMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGK 489

Query: 489 GISMPKVEPLEALCRARPI 500
           GI+MP   PL A+C+A PI
Sbjct: 490 GITMPMKIPLRAMCKALPI 495

BLAST of CsaV3_6G007560 vs. ExPASy Swiss-Prot
Match: P93147 (Isoflavone 2'-hydroxylase OS=Glycyrrhiza echinata OX=46348 GN=CYP81E1 PE=1 SV=2)

HSP 1 Score: 516.2 bits (1328), Expect = 4.3e-145
Identity = 256/509 (50.29%), Postives = 365/509 (71.71%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFI---QKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLS 62
           ME ++ L + + +LAL  IF I    +  KNLPP P +LPI+G+LH LK P+HRT   LS
Sbjct: 1   MEILSLLSYSVFYLALFFIFNIVIRARKFKNLPPGPPSLPIIGNLHHLKRPLHRTFKGLS 60

Query: 63  QKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPY 122
           +KYG +F+L  GSRLVVVVSS S  ++CFT ND+V ANRP F+SGKY+ Y  +TLG+  Y
Sbjct: 61  EKYGHVFSLWFGSRLVVVVSSASEFQQCFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSY 120

Query: 123 GDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELT 182
           G+HWRNLRR++A ++LS  R+N  + IR++E   LI +L     T F +++L S   ++T
Sbjct: 121 GEHWRNLRRITALDVLSNHRINSFSGIRRDETQRLITRLADDSSTNFAEMELSSRLYDMT 180

Query: 183 FNIIMRMLAGKRYYGE--EVSELEEAKKFREIMERAFQL--GSYPGDFLPFLKWVDWQ-Y 242
           FN IMRM++GKRYYGE  + S+L+EA +FR+++    QL   +   DF+P L+++D++  
Sbjct: 181 FNNIMRMISGKRYYGEDCDTSDLQEASQFRDMVSELLQLSGANNKTDFMPLLRFLDFENL 240

Query: 243 TKRVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIV 302
            KR++ +   TD FL+ L++EHR  +   E+ NTMI HLL LQ +QP+YYTD+IIKGL +
Sbjct: 241 EKRLKDISGKTDAFLRGLIEEHRTKK---ERANTMIDHLLNLQDSQPEYYTDQIIKGLAL 300

Query: 303 TILSAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVI 362
            +L A T++S+VT+EW+MSNLLNHPE LKKVKDELD  +GQDR++DE D+  L YL+NVI
Sbjct: 301 AMLLAGTDSSAVTLEWSMSNLLNHPEVLKKVKDELDTHVGQDRLVDESDLPKLTYLKNVI 360

Query: 363 SETLRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERF 422
           +ETLRLY PAPLL PH +S  C++GGY +P DT++++NAWAI RDP++W ++T+FKPERF
Sbjct: 361 NETLRLYTPAPLLLPHSTSDECNIGGYKVPQDTIVLINAWAIHRDPELWTEATTFKPERF 420

Query: 423 ESDHQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEI 482
           E   +  +           + FG+GRRACPG G+A R + +TL  LIQ F+WK ++  +I
Sbjct: 421 EKKGELEK----------LIAFGMGRRACPGEGLAIRAISMTLALLIQCFDWKLINGDKI 480

Query: 483 DMTEGQGISMPKVEPLEALCRARPIIKKL 504
           D+ E  G ++ K+ PL+A+C++RP+I K+
Sbjct: 481 DLAERDGFTLTKLVPLKAMCKSRPVINKV 496

BLAST of CsaV3_6G007560 vs. ExPASy Swiss-Prot
Match: Q6WNR0 (Isoflavone 2'-hydroxylase OS=Medicago truncatula OX=3880 GN=CYP81E7 PE=1 SV=1)

HSP 1 Score: 507.3 bits (1305), Expect = 2.0e-142
Identity = 245/503 (48.71%), Postives = 360/503 (71.57%), Query Frame = 0

Query: 6   ITYLPFLITFLALVSIFFIQKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLSQKYGPI 65
           + Y  F ++   ++ + F  +  KNLPP P +LPI+G+LH LK P++RT   L++KYG +
Sbjct: 7   LCYSLFYLSIFFIIRLLFQSRKFKNLPPGPTSLPIIGNLHHLKRPLNRTFKALTEKYGNV 66

Query: 66  FTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHWRN 125
            +L  GSRLVVVVSS+S  +ECFT ND+V ANRP F+SGKY+ Y  +TLG+  YG+HWRN
Sbjct: 67  ISLWFGSRLVVVVSSLSEFQECFTKNDVVLANRPRFLSGKYIFYNYTTLGSTSYGEHWRN 126

Query: 126 LRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTFNIIMR 185
           LRR+++ ++LS  R+N  A IR++E   LIKKL     T+F +V+L   F ++TFN IMR
Sbjct: 127 LRRITSLDVLSNHRINNFAPIRRDETQRLIKKLAEDSSTKFAEVELTFRFFDMTFNNIMR 186

Query: 186 MLAGKRYYGE--EVSELEEAKKFREIMERAFQL--GSYPGDFLPFLKWVDWQ-YTKRVES 245
           M++GKRYYG+  ++SE++EA +FR+++    QL   +   DF+P LK++D++   KRV+ 
Sbjct: 187 MISGKRYYGDDCDISEVQEASQFRDMVSELLQLSGANNKTDFMPLLKFLDFENLEKRVKR 246

Query: 246 LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 305
           +G+  D FL  L+ E R  +   E+ NTMI HLL +Q++QP+YYTD IIKGL + +L A 
Sbjct: 247 IGEKNDVFLSGLLQEQRSKK---ERTNTMIDHLLNMQESQPEYYTDTIIKGLCLAMLLAG 306

Query: 306 TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL 365
           T++S+VT+EW MSN+LN+PE LKKV+DE+D  +GQDR++DE D+  L YL+NVI ETLRL
Sbjct: 307 TDSSAVTLEWTMSNILNYPEVLKKVRDEVDTHVGQDRLVDESDLPKLTYLRNVIYETLRL 366

Query: 366 YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG 425
           Y PAPLL PH ++  C +GGY +P DT++++NAWAI RDP+ W ++T+FKPERF+   + 
Sbjct: 367 YTPAPLLLPHSTADECIMGGYKVPRDTIVLINAWAIHRDPETWSEATTFKPERFDKKGEL 426

Query: 426 REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQ 485
            +           + FG+GRRACPG G+A R + +TL  L+Q F+WKR+++++IDM+E  
Sbjct: 427 EK----------MIAFGMGRRACPGEGLALRAISMTLALLVQCFDWKRINDEKIDMSERD 486

Query: 486 GISMPKVEPLEALCRARPIIKKL 504
           G +M K+ PL+A+C+ RP++ K+
Sbjct: 487 GFTMTKLLPLKAMCKTRPVVNKV 496

BLAST of CsaV3_6G007560 vs. ExPASy TrEMBL
Match: A0A0A0K9E5 (Cytochrome P450 OS=Cucumis sativus OX=3659 GN=Csa6G088180 PE=2 SV=1)

HSP 1 Score: 1011.1 bits (2613), Expect = 1.6e-291
Identity = 507/507 (100.00%), Postives = 507/507 (100.00%), Query Frame = 0

Query: 1   MEMEAITYLPFLITFLALVSIFFIQKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLSQ 60
           MEMEAITYLPFLITFLALVSIFFIQKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLSQ
Sbjct: 1   MEMEAITYLPFLITFLALVSIFFIQKTKKNLPPSPLALPILGHLHLLKHPIHRTLHNLSQ 60

Query: 61  KYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYG 120
           KYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYG
Sbjct: 61  KYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYG 120

Query: 121 DHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTF 180
           DHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTF
Sbjct: 121 DHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTF 180

Query: 181 NIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVES 240
           NIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVES
Sbjct: 181 NIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVES 240

Query: 241 LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 300
           LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA
Sbjct: 241 LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 300

Query: 301 TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL 360
           TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL
Sbjct: 301 TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL 360

Query: 361 YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG 420
           YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG
Sbjct: 361 YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG 420

Query: 421 REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQ 480
           REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQ
Sbjct: 421 REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQ 480

Query: 481 GISMPKVEPLEALCRARPIIKKLALDD 508
           GISMPKVEPLEALCRARPIIKKLALDD
Sbjct: 481 GISMPKVEPLEALCRARPIIKKLALDD 507

BLAST of CsaV3_6G007560 vs. ExPASy TrEMBL
Match: A0A5A7TCR6 (Cytochrome P450 81E8-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G001720 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 9.4e-268
Identity = 474/509 (93.12%), Postives = 484/509 (95.09%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTK-----KNLPPSPLALPILGHLHLLKHPIHRTLHN 62
           ME   Y PFLIT LAL+SIFFI+K+K      NLPPSPL LPILGHLHLLKHPIHRTLH 
Sbjct: 1   METNAYFPFLITLLALISIFFIRKSKTKTKRNNLPPSPLPLPILGHLHLLKHPIHRTLHT 60

Query: 63  LSQKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 122
           LSQKYGPIFTLRLGSRLVVVVSS SA EECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA
Sbjct: 61  LSQKYGPIFTLRLGSRLVVVVSSTSAAEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 120

Query: 123 PYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSE 182
           PYGDHWRNLRRLSATE+LSTIRLNMSARIRKEEI ILIKKLNRV  T+FGKVKLKSLFSE
Sbjct: 121 PYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRVSSTDFGKVKLKSLFSE 180

Query: 183 LTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR 242
           LTFN+IMRM+AGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR
Sbjct: 181 LTFNMIMRMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR 240

Query: 243 VESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTIL 302
           VESLGKDTDWFLQNLVDEHR NEEEGEKGNTM+SHLL LQKTQPDYYTDEIIKGLIVTIL
Sbjct: 241 VESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQPDYYTDEIIKGLIVTIL 300

Query: 303 SAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISET 362
           SAATETSSVTIEWAMSNLLNHPEALKKVK+ELD QIGQDRILDEEDISNLPYLQNVISET
Sbjct: 301 SAATETSSVTIEWAMSNLLNHPEALKKVKEELDTQIGQDRILDEEDISNLPYLQNVISET 360

Query: 363 LRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESD 422
           LRLYPPAPLLAPHLSSSSCSLGGYHIPADT+LMVNAWAIQRDPKVWEDSTSFKPERFESD
Sbjct: 361 LRLYPPAPLLAPHLSSSSCSLGGYHIPADTILMVNAWAIQRDPKVWEDSTSFKPERFESD 420

Query: 423 HQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT 482
            Q REGS NNNNGY FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT
Sbjct: 421 -QVREGS-NNNNGYRFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT 480

Query: 483 EGQGISMPKVEPLEALCRARPIIKKLALD 507
           EGQGISMPKVEPLEALCRARPIIKKLALD
Sbjct: 481 EGQGISMPKVEPLEALCRARPIIKKLALD 507

BLAST of CsaV3_6G007560 vs. ExPASy TrEMBL
Match: A0A1S3CBC7 (cytochrome P450 81E8-like OS=Cucumis melo OX=3656 GN=LOC103498908 PE=3 SV=1)

HSP 1 Score: 929.9 bits (2402), Expect = 4.7e-267
Identity = 473/509 (92.93%), Postives = 483/509 (94.89%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTK-----KNLPPSPLALPILGHLHLLKHPIHRTLHN 62
           ME   Y PFLIT LAL+SIFFI+K+K      NLPPSPL LPILGHLHLLKHPIHRTLH 
Sbjct: 1   METNAYFPFLITLLALISIFFIRKSKTKTKRNNLPPSPLPLPILGHLHLLKHPIHRTLHT 60

Query: 63  LSQKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 122
           LSQKYGPIFTLRLGSRLVVVVSS SA EECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA
Sbjct: 61  LSQKYGPIFTLRLGSRLVVVVSSTSAAEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 120

Query: 123 PYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSE 182
           PYGDHWRNLRRLSATE+LSTIRLNMSARIRKEEI ILIKKLNRV  T+FGKVKLKSLFSE
Sbjct: 121 PYGDHWRNLRRLSATEVLSTIRLNMSARIRKEEISILIKKLNRVSSTDFGKVKLKSLFSE 180

Query: 183 LTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR 242
           LTFN+IM M+AGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR
Sbjct: 181 LTFNMIMTMVAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKR 240

Query: 243 VESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTIL 302
           VESLGKDTDWFLQNLVDEHR NEEEGEKGNTM+SHLL LQKTQPDYYTDEIIKGLIVTIL
Sbjct: 241 VESLGKDTDWFLQNLVDEHRSNEEEGEKGNTMVSHLLRLQKTQPDYYTDEIIKGLIVTIL 300

Query: 303 SAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISET 362
           SAATETSSVTIEWAMSNLLNHPEALKKVK+ELD QIGQDRILDEEDISNLPYLQNVISET
Sbjct: 301 SAATETSSVTIEWAMSNLLNHPEALKKVKEELDTQIGQDRILDEEDISNLPYLQNVISET 360

Query: 363 LRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESD 422
           LRLYPPAPLLAPHLSSSSCSLGGYHIPADT+LMVNAWAIQRDPKVWEDSTSFKPERFESD
Sbjct: 361 LRLYPPAPLLAPHLSSSSCSLGGYHIPADTILMVNAWAIQRDPKVWEDSTSFKPERFESD 420

Query: 423 HQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT 482
            Q REGS NNNNGY FLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT
Sbjct: 421 -QVREGS-NNNNGYRFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMT 480

Query: 483 EGQGISMPKVEPLEALCRARPIIKKLALD 507
           EGQGISMPKVEPLEALCRARPIIKKLALD
Sbjct: 481 EGQGISMPKVEPLEALCRARPIIKKLALD 507

BLAST of CsaV3_6G007560 vs. ExPASy TrEMBL
Match: A0A6J1GPQ4 (cytochrome P450 81E8-like OS=Cucurbita moschata OX=3662 GN=LOC111456346 PE=3 SV=1)

HSP 1 Score: 781.6 bits (2017), Expect = 2.0e-222
Identity = 393/504 (77.98%), Postives = 442/504 (87.70%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTK---KNLPPSPLALPILGHLHLLKHPIHRTLHNLS 62
           ME  +Y+  LITFLA+ SIF +QK K   KNLPP+P ALPILGHLHLLKHP+HRTL +L+
Sbjct: 1   METSSYVAALITFLAVGSIFLLQKRKNRAKNLPPTPWALPILGHLHLLKHPVHRTLQSLA 60

Query: 63  QKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPY 122
           QK+GPIFTLRLGSRL VVVSS  A+EECFTTNDIVFANRP+FVSG YL+YGN+T+GAAPY
Sbjct: 61  QKHGPIFTLRLGSRLAVVVSSAPAVEECFTTNDIVFANRPQFVSGMYLSYGNTTMGAAPY 120

Query: 123 GDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELT 182
           GDHWRNLRRLS+TEILS+IRLNMSA IRKEE+ IL++KL +    +FGKVKLK+LFSELT
Sbjct: 121 GDHWRNLRRLSSTEILSSIRLNMSAGIRKEEVNILLRKLYQFSRHDFGKVKLKTLFSELT 180

Query: 183 FNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVE 242
           FNIIMRM+AGKRYYG+EV+ELEEAK+FR+IME +F+LGSYPGDFLPFLKWVD QY KR++
Sbjct: 181 FNIIMRMVAGKRYYGDEVTELEEAKEFRQIMETSFRLGSYPGDFLPFLKWVDRQYPKRMK 240

Query: 243 SLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSA 302
           SLG+  D  LQNLVDEHRR + EG+K +TMISHLLCLQ++QP+YY+DEIIKGLIVTIL+A
Sbjct: 241 SLGRAIDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEIIKGLIVTILTA 300

Query: 303 ATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLR 362
           ATETSSVTIEWAMSNLLNHPEAL K + ELD QIGQDR+L+EEDI NLPYLQNVISETLR
Sbjct: 301 ATETSSVTIEWAMSNLLNHPEALNKARAELDAQIGQDRLLEEEDIPNLPYLQNVISETLR 360

Query: 363 LYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQ 422
           LYP APLLAPH SS SCSLGGYHIPA T L+VNAWA+QRDP+VWED TSFKPERFES   
Sbjct: 361 LYPAAPLLAPHFSSESCSLGGYHIPAGTTLLVNAWAVQRDPEVWEDPTSFKPERFESGGV 420

Query: 423 GREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEG 482
                    N Y FLPFGLGRRACPGMGMANRVVGL LGSLIQSFEWKRVS++E+DMTE 
Sbjct: 421 AA-------NAYKFLPFGLGRRACPGMGMANRVVGLALGSLIQSFEWKRVSDEELDMTES 480

Query: 483 QGISMPKVEPLEALCRARPIIKKL 504
           QGISM KVEPLEALCRARPIIK L
Sbjct: 481 QGISMYKVEPLEALCRARPIIKNL 497

BLAST of CsaV3_6G007560 vs. ExPASy TrEMBL
Match: A0A6J1JR43 (cytochrome P450 81E8-like OS=Cucurbita maxima OX=3661 GN=LOC111488144 PE=3 SV=1)

HSP 1 Score: 775.4 bits (2001), Expect = 1.5e-220
Identity = 389/505 (77.03%), Postives = 439/505 (86.93%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTK---KNLPPSPLALPILGHLHLLKHPIHRTLHNLS 62
           ME  +Y   L+T LAL SIF +QK K   KNLPPSP ALPILGHLHLLKHP+HRTL +L+
Sbjct: 1   METSSYFAALVTLLALGSIFLLQKRKNRAKNLPPSPWALPILGHLHLLKHPVHRTLQSLA 60

Query: 63  QKYGPIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPY 122
           QK+GPIFTLRLGSRL +VVSS  A+EECFTTNDIVFANRP+FVSG YL+YGN+T+GAAPY
Sbjct: 61  QKHGPIFTLRLGSRLAIVVSSAPAVEECFTTNDIVFANRPQFVSGMYLSYGNTTMGAAPY 120

Query: 123 GDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELT 182
           GDHWRNLRRLS+TEILS+IRLNMSA IRKEE+ IL++KL +    +FGKVKLK+LFSELT
Sbjct: 121 GDHWRNLRRLSSTEILSSIRLNMSAGIRKEEVNILLRKLYQFSRHDFGKVKLKTLFSELT 180

Query: 183 FNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPGDFLPFLKWVDWQYTKRVE 242
           FNIIMRM+AGKRY+G+EV+ELEEAK+FREIME +F+LGSYPGDFLPFLKWVD QY KR++
Sbjct: 181 FNIIMRMVAGKRYFGDEVTELEEAKEFREIMETSFRLGSYPGDFLPFLKWVDRQYPKRMK 240

Query: 243 SLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSA 302
           SLG+  D  LQNLVDEHRR + EG+K +TMISHLLCLQ++QP+YY+DEIIKGLIVTIL+A
Sbjct: 241 SLGRALDSLLQNLVDEHRRYQNEGKKLDTMISHLLCLQESQPEYYSDEIIKGLIVTILTA 300

Query: 303 ATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLR 362
           ATETSSVTIEWAMSNLLNHPEAL K + ELD QIGQDR+L+EEDI  LPYLQNVISETLR
Sbjct: 301 ATETSSVTIEWAMSNLLNHPEALNKARAELDAQIGQDRLLEEEDIPTLPYLQNVISETLR 360

Query: 363 LYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQ 422
           LYP APLLAPH SS SCSLGGYHIPA T ++VNAWA+QRDP+VWED T FKPERFES   
Sbjct: 361 LYPAAPLLAPHFSSESCSLGGYHIPAGTTMLVNAWAVQRDPEVWEDPTRFKPERFESGGV 420

Query: 423 GREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEG 482
                    N Y FLPFGLGRRACPGMGMANRVVGL LGSLIQSFEWKRVS++E+DMTE 
Sbjct: 421 AA-------NAYKFLPFGLGRRACPGMGMANRVVGLALGSLIQSFEWKRVSDEELDMTES 480

Query: 483 QGISMPKVEPLEALCRARPIIKKLA 505
           QGISM KVEPLEALCRARPI+K LA
Sbjct: 481 QGISMYKVEPLEALCRARPIMKNLA 498

BLAST of CsaV3_6G007560 vs. TAIR 10
Match: AT4G37360.1 (cytochrome P450, family 81, subfamily D, polypeptide 2 )

HSP 1 Score: 527.7 bits (1358), Expect = 1.0e-149
Identity = 273/512 (53.32%), Postives = 365/512 (71.29%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTKK--NLPPSPL-ALPILGHLHLLKHPIHRTLHNLS 62
           MEA+  L F   F+ L  IF I + K+  NLPPSP  ALP++GHL LLK P+HR   ++S
Sbjct: 1   MEAL-MLIFTFCFIVLSLIFLIGRIKRKLNLPPSPAWALPVIGHLRLLKPPLHRVFLSVS 60

Query: 63  QKYG--PIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 122
           Q  G  PI +LRLG+RL+ VVSS S  EECFT ND++ ANR   +S K+++YGNST+ +A
Sbjct: 61  QSLGDAPIISLRLGNRLLFVVSSHSIAEECFTKNDVILANRQTTISTKHISYGNSTVVSA 120

Query: 123 PYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSE 182
            Y +HWRNLRR+ A EI S  RLN  + IR++EI  LI +L R     F KV++KS+FS+
Sbjct: 121 SYSEHWRNLRRIGALEIFSAHRLNSFSSIRRDEIRRLIGRLLRNSSYGFTKVEMKSMFSD 180

Query: 183 LTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPG---DFLPFLKWVDWQY 242
           LTFN I+RMLAGK YYG+   +  EAK+ R ++  A    S PG   D++P L W+ +  
Sbjct: 181 LTFNNIIRMLAGKCYYGDGKEDDPEAKRVRTLIAEAMS-SSGPGNAADYIPILTWITYSE 240

Query: 243 TKRVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIV 302
           T R++ L    D FLQ LVDE R  +E  +K NTM+ HLLCLQ+TQP+YY D IIKG ++
Sbjct: 241 T-RIKKLAGRLDEFLQGLVDEKREGKE--KKENTMVDHLLCLQETQPEYYMDRIIKGTML 300

Query: 303 TILSAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVI 362
           ++++  T+T++VT+EWA+S+LLN+PE L K +DE+D+ IG DR+L+E DI NLPYLQN++
Sbjct: 301 SLIAGGTDTTAVTLEWALSSLLNNPEVLNKARDEIDRMIGVDRLLEESDIPNLPYLQNIV 360

Query: 363 SETLRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERF 422
           SETLRLYP AP+L PH++S  C +GGY +P  TML+ NAWAI RDP +W+D TSFKPERF
Sbjct: 361 SETLRLYPAAPMLLPHVASKDCKVGGYDMPRGTMLLTNAWAIHRDPLLWDDPTSFKPERF 420

Query: 423 ESDHQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEI 482
           E + + ++           +PFGLGRRACPG G+A R+V L+LGSLIQ FEW+R+ E+E+
Sbjct: 421 EKEGEAKK----------LMPFGLGRRACPGSGLAQRLVTLSLGSLIQCFEWERIGEEEV 480

Query: 483 DMTEGQGISMPKVEPLEALCRARPIIKKLALD 507
           DMTEG G++MPK  PLEA+CRAR  + K+  D
Sbjct: 481 DMTEGPGLTMPKARPLEAMCRARDFVGKILPD 497

BLAST of CsaV3_6G007560 vs. TAIR 10
Match: AT4G37370.1 (cytochrome P450, family 81, subfamily D, polypeptide 8 )

HSP 1 Score: 525.4 bits (1352), Expect = 5.1e-149
Identity = 271/502 (53.98%), Postives = 358/502 (71.31%), Query Frame = 0

Query: 9   LPFLITFLALVSIFFIQKTKK--NLPPSPL-ALPILGHLHLLKHPIHRTLHNLSQKY--G 68
           L F I F+ L  I+ I K K+  NLPPSP  +LP++GHL LLK PIHRT  +LSQ     
Sbjct: 6   LIFSILFVVLSLIYLIGKLKRKPNLPPSPAWSLPVIGHLRLLKPPIHRTFLSLSQSLNNA 65

Query: 69  PIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHW 128
           PIF+LRLG+RLV V SS S  EECFT ND+V ANRP F+  K++ Y  +T+ AA YGDHW
Sbjct: 66  PIFSLRLGNRLVFVNSSHSIAEECFTKNDVVLANRPNFILAKHVAYDYTTMIAASYGDHW 125

Query: 129 RNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTFNII 188
           RNLRR+ + EI S  RLN    IRK+EI  L+ +L+R    EF KV +KS+ S+LTFN I
Sbjct: 126 RNLRRIGSVEIFSNHRLNSFLSIRKDEIRRLVFRLSRNFSQEFVKVDMKSMLSDLTFNNI 185

Query: 189 MRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGSYPG--DFLPFLKWVDWQYTKRVESL 248
           +RM+AGKRYYG+ V +  EAK+ R+++             D+LP L+ V   Y  RV+ L
Sbjct: 186 LRMVAGKRYYGDGVEDDPEAKRVRQLIADVVACAGAGNAVDYLPVLRLVS-DYETRVKKL 245

Query: 249 GKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAAT 308
               D FLQ LVDE R   E  EKGNTMI HLL LQ++QPDY+TD IIKG ++ ++ A T
Sbjct: 246 AGRLDEFLQGLVDEKR---EAKEKGNTMIDHLLTLQESQPDYFTDRIIKGNMLALILAGT 305

Query: 309 ETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRLY 368
           +TS+VT+EWA+SN+LNHP+ L K +DE+D++IG DR++DE DISNLPYLQN++SETLRLY
Sbjct: 306 DTSAVTLEWALSNVLNHPDVLNKARDEIDRKIGLDRLMDESDISNLPYLQNIVSETLRLY 365

Query: 369 PPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQGR 428
           P AP+L PH++S  C + GY +P  T+L+ N WAI RDP++W+D  SFKPERFE + + +
Sbjct: 366 PAAPMLLPHVASEDCKVAGYDMPRGTILLTNVWAIHRDPQLWDDPMSFKPERFEKEGEAQ 425

Query: 429 EGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTEGQG 488
           +           +PFGLGRRACPG G+A+R++ LTLGSLIQ  EW+++ E E+DM+EG+G
Sbjct: 426 K----------LMPFGLGRRACPGSGLAHRLINLTLGSLIQCLEWEKIGE-EVDMSEGKG 485

Query: 489 ISMPKVEPLEALCRARPIIKKL 504
           ++MPK +PLEA+CRARP + K+
Sbjct: 486 VTMPKAKPLEAMCRARPSVVKI 492

BLAST of CsaV3_6G007560 vs. TAIR 10
Match: AT4G37340.1 (cytochrome P450, family 81, subfamily D, polypeptide 3 )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 264/504 (52.38%), Postives = 359/504 (71.23%), Query Frame = 0

Query: 9   LPFLITFLALVSIFFIQKTKK--NLPPSP-LALPILGHLHLLKHPIHRTLHNLSQKYG-- 68
           L F   F++L   F I + K+  NLPPSP  ALP++GHL LLK P+HR   ++S+  G  
Sbjct: 6   LIFTFLFISLSLTFIIGRIKRRPNLPPSPSWALPVIGHLRLLKPPLHRVFLSVSESLGDA 65

Query: 69  PIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAAPYGDHW 128
           PI +LRLG+RLV VVSS S  EECFT ND+V ANR   ++ K+++YG +T+  A YGDHW
Sbjct: 66  PIISLRLGNRLVFVVSSHSLAEECFTKNDVVLANRFNSLASKHISYGCTTVVTASYGDHW 125

Query: 129 RNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSELTFNII 188
           RNLRR+ A EI S  RLN  + IR++EI  LI  L+R    EF KV++KS+FS LTFN I
Sbjct: 126 RNLRRIGAVEIFSAHRLNSFSSIRRDEIHRLIACLSRNSSLEFTKVEMKSMFSNLTFNNI 185

Query: 189 MRMLAGKRYYGEEVSELEEAKKFREIMER---AFQLGSYPGDFLPFLKWVDWQYTKRVES 248
           +RMLAGK YYG+   +  EAK+ RE++      F  G+   D+LP L W+     KR++ 
Sbjct: 186 IRMLAGKCYYGDGAEDDPEAKRVRELIAEGMGCFGAGN-TADYLPILTWITGS-EKRIKK 245

Query: 249 LGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTILSAA 308
           +    D FLQ LVDE R  +E  ++ NTM+ HLLCLQ+TQP+YYTD IIKG++++++ A 
Sbjct: 246 IASRLDEFLQGLVDERREGKE--KRQNTMVDHLLCLQETQPEYYTDNIIKGIMLSLILAG 305

Query: 309 TETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISETLRL 368
           T+TS+VT+EW +S LLNHP+ L K +DE+D ++G +R+++E D+S+LPYLQN++SE+LRL
Sbjct: 306 TDTSAVTLEWTLSALLNHPQILSKARDEIDNKVGLNRLVEESDLSHLPYLQNIVSESLRL 365

Query: 369 YPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFESDHQG 428
           YP +PLL PH++S  C +GGYH+P  TML+ NAWAI RDPK+W+D TSFKPERFE + + 
Sbjct: 366 YPASPLLVPHVASEDCKVGGYHMPRGTMLLTNAWAIHRDPKIWDDPTSFKPERFEKEGEA 425

Query: 429 REGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDMTE-G 488
           ++           L FGLGRRACPG G+A R+  LT+GSLIQ FEW+R+ E+E+DMTE G
Sbjct: 426 QK----------LLGFGLGRRACPGSGLAQRLASLTIGSLIQCFEWERIGEEEVDMTEGG 485

Query: 489 QGISMPKVEPLEALCRARPIIKKL 504
            G+ MPK  PL A+C+ARP++ K+
Sbjct: 486 GGVIMPKAIPLVAMCKARPVVGKI 495

BLAST of CsaV3_6G007560 vs. TAIR 10
Match: AT4G37330.1 (cytochrome P450, family 81, subfamily D, polypeptide 4 )

HSP 1 Score: 509.6 bits (1311), Expect = 2.9e-144
Identity = 267/507 (52.66%), Postives = 348/507 (68.64%), Query Frame = 0

Query: 1   MEMEAITYLPFLITFLALVSIFFIQKTKKNLPPSP-LALPILGHLHLLKHPIHRTLHNLS 60
           M++  I  L FL  F   + +    K K NLPPSP ++LP++GHLHLLK P+HRT  +LS
Sbjct: 1   MDLNQILILSFLSLFTLAIFLLTRSKRKLNLPPSPAISLPVIGHLHLLKPPLHRTFLSLS 60

Query: 61  QKYG--PIFTLRLGSRLVVVVSSISAIEECFTTNDIVFANRPEFVSGKYLTYGNSTLGAA 120
           +  G  P+F LRLG+RLV V+SS S  EECFT ND+V ANRP+F   K+L Y  + L +A
Sbjct: 61  KSIGNAPVFHLRLGNRLVYVISSRSIAEECFTKNDVVLANRPKFTISKHLGYNATYLLSA 120

Query: 121 PYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLFSE 180
            YGDHWRNLRR++A EI ST RLN    IRK+EI  LI  L+R     F +V++K+L + 
Sbjct: 121 SYGDHWRNLRRIAAVEIFSTHRLNSFLYIRKDEIRRLISHLSRDSLHGFVEVEMKTLLTN 180

Query: 181 LTFNIIMRMLAGKRYYGEEVSELEEAKKFREIMERAFQLGS-YPGDFLPFLKWVDWQYTK 240
           L  N  +RMLAGKRY+GE+  + +  K    + E     G+  P D+L  L+WV   Y K
Sbjct: 181 LASNTTIRMLAGKRYFGEDNDDAKLVKNL--VSEAVTSAGAGNPIDYLSILRWVS-SYEK 240

Query: 241 RVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLIVTI 300
           R+++LG   D FLQ LVDE R    E EKG TMI HLL LQ  QPDYYTD IIKG+I+T+
Sbjct: 241 RIKNLGNRFDTFLQKLVDEKR---AEKEKGETMIDHLLALQDIQPDYYTDVIIKGIILTL 300

Query: 301 LSAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNVISE 360
           + A T+TSSVT+EWAMSNLLNHPE LKK + E+D+++G DR++DE DI NL YLQ+++ E
Sbjct: 301 IIAGTDTSSVTLEWAMSNLLNHPEILKKARMEIDEKVGLDRLVDESDIVNLSYLQSIVLE 360

Query: 361 TLRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPERFES 420
           TLR+YP  PLL PHLSS  C +GGY IP+ TM++ NAWA+ RDP+VWED   FKPERFE 
Sbjct: 361 TLRMYPAVPLLLPHLSSEDCKVGGYDIPSGTMVLTNAWAMHRDPEVWEDPEIFKPERFEK 420

Query: 421 DHQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKEIDM 480
           + +  +           + FG+GRRACPG G+A+R++   LGSL+Q FEW+RV E  +DM
Sbjct: 421 EGEAEK----------LISFGMGRRACPGAGLAHRLINQALGSLVQCFEWERVGEDFVDM 480

Query: 481 TEGQGISMPKVEPLEALCRARPIIKKL 504
           TE +G ++PK  PL A+C+AR I+ KL
Sbjct: 481 TEDKGATLPKAIPLRAMCKARSIVDKL 491

BLAST of CsaV3_6G007560 vs. TAIR 10
Match: AT5G36220.1 (cytochrome p450 81d1 )

HSP 1 Score: 504.2 bits (1297), Expect = 1.2e-142
Identity = 260/510 (50.98%), Postives = 351/510 (68.82%), Query Frame = 0

Query: 3   MEAITYLPFLITFLALVSIFFIQKTKKNLPPSPLA-LPILGHLHLLKHPIHRTLHNLSQK 62
           +  + Y  F + FL ++S  F++  K+NLPPSP   LPI+GHL LLK PIHRTL + S+ 
Sbjct: 6   IRVVLYSIFSLIFL-IISFKFLKPKKQNLPPSPPGWLPIIGHLRLLKPPIHRTLRSFSET 65

Query: 63  Y-----GPIFTLRLGSRLVVVVSSIS-AIEECFTTNDIVFANRPEFVSGKYLTYGNSTLG 122
                 G + +LRLGSRLV VVSS   A EECF  ND+V ANRP+ + GK++ Y N+ + 
Sbjct: 66  LDHNDGGGVMSLRLGSRLVYVVSSHKVAAEECFGKNDVVLANRPQVIIGKHVGYNNTNMI 125

Query: 123 AAPYGDHWRNLRRLSATEILSTIRLNMSARIRKEEIGILIKKLNRVLGTEFGKVKLKSLF 182
           AAPYGDHWRNLRRL   EI ST RLN    +R +E+  LI +L+R+ GT+   V+LK + 
Sbjct: 126 AAPYGDHWRNLRRLCTIEIFSTHRLNCFLYVRTDEVRRLISRLSRLAGTKKTVVELKPML 185

Query: 183 SELTFNIIMRMLAGKRYYGEEVSELEEAKKFREIME--RAFQLGSYPGDFLPFLKWVDWQ 242
            +LTFN IMRM+ GKRYYGEE ++ EEAK+ R+++    A        D++P L+     
Sbjct: 186 MDLTFNNIMRMMTGKRYYGEETTDEEEAKRVRKLVADVGANTSSGNAVDYVPILRLFS-S 245

Query: 243 YTKRVESLGKDTDWFLQNLVDEHRRNEEEGEKGNTMISHLLCLQKTQPDYYTDEIIKGLI 302
           Y  RV+ LG++TD FLQ L+D+ R  +   E G TMI HLL LQK+  +YYTD+IIKG+I
Sbjct: 246 YENRVKKLGEETDKFLQGLIDDKRGQQ---ETGTTMIDHLLVLQKSDIEYYTDQIIKGII 305

Query: 303 VTILSAATETSSVTIEWAMSNLLNHPEALKKVKDELDKQIGQDRILDEEDISNLPYLQNV 362
           + ++ A T TS+VT+EWA+SNLLNHP+ + K +DE+D ++G DR+++E D+S LPYL+N+
Sbjct: 306 LIMVIAGTNTSAVTLEWALSNLLNHPDVISKARDEIDNRVGLDRLIEEADLSELPYLKNI 365

Query: 363 ISETLRLYPPAPLLAPHLSSSSCSLGGYHIPADTMLMVNAWAIQRDPKVWEDSTSFKPER 422
           + ETLRL+P  PLL PH++S  C +G Y +P  T L+VNAWAI RDP  W+D  SFKPER
Sbjct: 366 VLETLRLHPATPLLVPHMASEDCKIGSYDMPRGTTLLVNAWAIHRDPNTWDDPDSFKPER 425

Query: 423 FESDHQGREGSNNNNNGYSFLPFGLGRRACPGMGMANRVVGLTLGSLIQSFEWKRVSEKE 482
           FE + + ++           L FGLGRRACPG G+A R+VGL LGSLIQ FEW+RV   E
Sbjct: 426 FEKEEEAQK----------LLAFGLGRRACPGSGLAQRIVGLALGSLIQCFEWERVGNVE 485

Query: 483 IDMTEGQGISMPKVEPLEALCRARPIIKKL 504
           +DM EG G ++PK  PL+A+C+ARP + K+
Sbjct: 486 VDMKEGVGNTVPKAIPLKAICKARPFLHKI 500