CsaV3_6G003270 (gene) Cucumber (Chinese Long) v3

Overview
NameCsaV3_6G003270
Typegene
OrganismCucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
DescriptionMannan endo-1,4-beta-mannosidase
Locationchr6: 2462082 .. 2464013 (+)
RNA-Seq ExpressionCsaV3_6G003270
SyntenyCsaV3_6G003270
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTATATAAGCAATGTTTGGGCTGTGCTTCTCTCGCCACAATCCAAGAAGTGGATATTCAATTGAAAAAAAGATCCGAGAAGATGGAAAGAACTATTCAAGTCATTCTTCTTCTAGCCCTAGTTTCAGTGTTTTCATCTTTTTCAGCTTATTCCTTACCCTTGTCAACACATGGAAGATGGATCATTGATTCACAATCTGGAAAAAGAGTGAAACTCGTGTGTGTGAATTGGCCTTCCCACACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCATTAAAAGAACTAGCAGACGAAGCAATCAAGTTAAGGTTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTATGCAAATAGGACAGTTGAAGAAAACTTTGATCTTCTTGATTTAGAACAAGCAAAAGCTGGATTGGCTCAATACAATCCTTTTGTGTTGAACAAGACTATTGCCGAAGCTTATGAAGCAGTTGTTGATGTGTTGGGGGCAAGTGGTCTAATGGTGATTGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTATGTAACATCAAGAGATTTTCTTATCATTTTTTCTTAAAACTAAACTGAAGCATTTAAAAATTTCTATAATATTTTCATTAAACAAAGAATTTTTTTTGTGGTTGTTTAGGTGGTAGGGATGAGTCTACGAAATGAGTTACGAGGAATGATGGAAAATGCAAATGATTGGAACAATTATGTAACTCAAGGAGTAACCACGATCCATAAAATAAATCCAGCAGTTTTAGTGATTGTTTCAGGTTTAAATTATGATAATGATCTTCGATGCTTAAAGGATAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGCTTTTGAGGTGCACCTTTATTCTTTCAGTGGAGATTCTGAGAGCAAGTTTGTGCAACAGCCATTGAACAATATTTGTGCAAAAATTATGCATGAATTTATAGATCATGCTGAGTTTGTAATTGAAGGACCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCGGAAAATCGATTCATGAGTTGTTTCACAGCCCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGTCAAGCAGAACTTGCGGAAACGTTTGGAGTGCTTGACTCTAATTGGACTCAAATTAAGAACCCTAACTTTGTCCAGAAGTTTCAACTATTGCAAACCATGTTGCAAGGTAACTATACTAATTATTTTCAATGTTGTGATTAGGGAAAGTTAATGTTAGATAGTTCAATTAGTGTTTTTGTAAAAAAGTAACAATTTTGATAACATTTTTTTTATGGTATGCAGATCCATATTCCAATGCATCGTTCTCATATGTTATATATCATGTACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCTCAAGTCGATGGAGTCATGACAATGATAGTACTCCAATCAAGATGTCAAGCACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGTCTGGAGGCATCTCTTTCAACTGACTGCATAGGCAAACAAAGCCTTTGGAGTGCCATTTCAAATTCTAACCTTCATTTGGGAACTGTCACAGAAGATGGAAAAAGTCTTTGTCTACAGATTATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACAACCAATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGCAACCAACACATTACTGTGA

mRNA sequence

ATGGTATATAAGCAATGTTTGGGCTGTGCTTCTCTCGCCACAATCCAAGAAGTGGATATTCAATTGAAAAAAAGATCCGAGAAGATGGAAAGAACTATTCAAGTCATTCTTCTTCTAGCCCTAGTTTCAGTGTTTTCATCTTTTTCAGCTTATTCCTTACCCTTGTCAACACATGGAAGATGGATCATTGATTCACAATCTGGAAAAAGAGTGAAACTCGTGTGTGTGAATTGGCCTTCCCACACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCATTAAAAGAACTAGCAGACGAAGCAATCAAGTTAAGGTTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTATGCAAATAGGACAGTTGAAGAAAACTTTGATCTTCTTGATTTAGAACAAGCAAAAGCTGGATTGGCTCAATACAATCCTTTTGTGTTGAACAAGACTATTGCCGAAGCTTATGAAGCAGTTGTTGATGTGTTGGGGGCAAGTGGTCTAATGGTGATTGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTGGTAGGGATGAGTCTACGAAATGAGTTACGAGGAATGATGGAAAATGCAAATGATTGGAACAATTATGTAACTCAAGGAGTAACCACGATCCATAAAATAAATCCAGCAGTTTTAGTGATTGTTTCAGGTTTAAATTATGATAATGATCTTCGATGCTTAAAGGATAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGCTTTTGAGGTGCACCTTTATTCTTTCAGTGGAGATTCTGAGAGCAAGTTTGTGCAACAGCCATTGAACAATATTTGTGCAAAAATTATGCATGAATTTATAGATCATGCTGAGTTTGTAATTGAAGGACCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCGGAAAATCGATTCATGAGTTGTTTCACAGCCCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGTCAAGCAGAACTTGCGGAAACGTTTGGAGTGCTTGACTCTAATTGGACTCAAATTAAGAACCCTAACTTTGTCCAGAAGTTTCAACTATTGCAAACCATGTTGCAAGATCCATATTCCAATGCATCGTTCTCATATGTTATATATCATGTACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCTCAAGTCGATGGAGTCATGACAATGATAGTACTCCAATCAAGATGTCAAGCACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGTCTGGAGGCATCTCTTTCAACTGACTGCATAGGCAAACAAAGCCTTTGGAGTGCCATTTCAAATTCTAACCTTCATTTGGGAACTGTCACAGAAGATGGAAAAAGTCTTTGTCTACAGATTATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACAACCAATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGCAACCAACACATTACTGTGA

Coding sequence (CDS)

ATGGTATATAAGCAATGTTTGGGCTGTGCTTCTCTCGCCACAATCCAAGAAGTGGATATTCAATTGAAAAAAAGATCCGAGAAGATGGAAAGAACTATTCAAGTCATTCTTCTTCTAGCCCTAGTTTCAGTGTTTTCATCTTTTTCAGCTTATTCCTTACCCTTGTCAACACATGGAAGATGGATCATTGATTCACAATCTGGAAAAAGAGTGAAACTCGTGTGTGTGAATTGGCCTTCCCACACACAAAGCATGTTGATAGAAGGTCTCAACCATAGGCCATTAAAAGAACTAGCAGACGAAGCAATCAAGTTAAGGTTCAATTGTGTACGACTCACATATGCAACCCACATGTTCACTCGCTATGCAAATAGGACAGTTGAAGAAAACTTTGATCTTCTTGATTTAGAACAAGCAAAAGCTGGATTGGCTCAATACAATCCTTTTGTGTTGAACAAGACTATTGCCGAAGCTTATGAAGCAGTTGTTGATGTGTTGGGGGCAAGTGGTCTAATGGTGATTGCTGACAATCACATGAGCCAACCAAGATGGTGTTGCTCTCTTGACGATGGGAATGGCTTCTTTGGAAACCGCTATTTTGACCCTCAAGAATGGCTACAAGGTCTTAGCTTAGTTGCTCAACGCTTTAACAACAAATCCACGGTGGTAGGGATGAGTCTACGAAATGAGTTACGAGGAATGATGGAAAATGCAAATGATTGGAACAATTATGTAACTCAAGGAGTAACCACGATCCATAAAATAAATCCAGCAGTTTTAGTGATTGTTTCAGGTTTAAATTATGATAATGATCTTCGATGCTTAAAGGATAAGCCTTTGAATGTAAGCACCTTAGACAATAAGTTGGCTTTTGAGGTGCACCTTTATTCTTTCAGTGGAGATTCTGAGAGCAAGTTTGTGCAACAGCCATTGAACAATATTTGTGCAAAAATTATGCATGAATTTATAGATCATGCTGAGTTTGTAATTGAAGGACCAAACCCATTTCCTTTATTTGTTAGTGAATATGGGTATGATCAAAGAGAGGTTGACGATGCGGAAAATCGATTCATGAGTTGTTTCACAGCCCATCTTGCACAAAAAGATTTGGATTGGGCATTGTGGACATGGCAAGGTAGTTATTATTATAGAGAAGGTCAAGCAGAACTTGCGGAAACGTTTGGAGTGCTTGACTCTAATTGGACTCAAATTAAGAACCCTAACTTTGTCCAGAAGTTTCAACTATTGCAAACCATGTTGCAAGATCCATATTCCAATGCATCGTTCTCATATGTTATATATCATGTACAAAGTGGGCAATGTATCGAAGTCTCTAATGACAACAAAGAAATTTTCCTCACCAATTGCTCCACCTCAAGTCGATGGAGTCATGACAATGATAGTACTCCAATCAAGATGTCAAGCACTGGTTTGTGCTTGAAGGCTAGTGGAGAAGGTCTGGAGGCATCTCTTTCAACTGACTGCATAGGCAAACAAAGCCTTTGGAGTGCCATTTCAAATTCTAACCTTCATTTGGGAACTGTCACAGAAGATGGAAAAAGTCTTTGTCTACAGATTATTGAAAGCTCAAATTCTTCAAAAATTGTGACCAACTCTTGTATTTGCACAACCAATGATCCAACTTGCCTCCAAGACACACAAAGCCAATGGTTTGAACTTGTTGCAACCAACACATTACTGTGA

Protein sequence

MVYKQCLGCASLATIQEVDIQLKKRSEKMERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTLL*
Homology
BLAST of CsaV3_6G003270 vs. NCBI nr
Match: KAE8646606.1 (hypothetical protein Csa_005432 [Cucumis sativus])

HSP 1 Score: 1156.7 bits (2991), Expect = 0.0e+00
Identity = 567/567 (100.00%), Postives = 567/567 (100.00%), Query Frame = 0

Query: 1   MVYKQCLGCASLATIQEVDIQLKKRSEKMERTIQVILLLALVSVFSSFSAYSLPLSTHGR 60
           MVYKQCLGCASLATIQEVDIQLKKRSEKMERTIQVILLLALVSVFSSFSAYSLPLSTHGR
Sbjct: 1   MVYKQCLGCASLATIQEVDIQLKKRSEKMERTIQVILLLALVSVFSSFSAYSLPLSTHGR 60

Query: 61  WIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFT 120
           WIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFT
Sbjct: 61  WIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLTYATHMFT 120

Query: 121 RYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMS 180
           RYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMS
Sbjct: 121 RYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMS 180

Query: 181 QPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNELRGMMENAND 240
           QPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNELRGMMENAND
Sbjct: 181 QPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNELRGMMENAND 240

Query: 241 WNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSG 300
           WNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSG
Sbjct: 241 WNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSG 300

Query: 301 DSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREVDDAENRFMSC 360
           DSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREVDDAENRFMSC
Sbjct: 301 DSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREVDDAENRFMSC 360

Query: 361 FTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFVQKFQLLQTML 420
           FTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFVQKFQLLQTML
Sbjct: 361 FTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFVQKFQLLQTML 420

Query: 421 QDPYSNASFSYVIYHVQSGQCIEVSNDNKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCL 480
           QDPYSNASFSYVIYHVQSGQCIEVSNDNKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCL
Sbjct: 421 QDPYSNASFSYVIYHVQSGQCIEVSNDNKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCL 480

Query: 481 KASGEGLEASLSTDCIGKQSLWSAISNSNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSC 540
           KASGEGLEASLSTDCIGKQSLWSAISNSNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSC
Sbjct: 481 KASGEGLEASLSTDCIGKQSLWSAISNSNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSC 540

Query: 541 ICTTNDPTCLQDTQSQWFELVATNTLL 568
           ICTTNDPTCLQDTQSQWFELVATNTLL
Sbjct: 541 ICTTNDPTCLQDTQSQWFELVATNTLL 567

BLAST of CsaV3_6G003270 vs. NCBI nr
Match: XP_011658389.1 (glycosyl hydrolase 5 family protein [Cucumis sativus])

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 29  MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 88
           MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 60

Query: 89  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 148
           GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 120

Query: 149 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 208
           FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180

Query: 209 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 268
           LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY
Sbjct: 181 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 240

Query: 269 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 328
           DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF
Sbjct: 241 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 300

Query: 329 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 388
           VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 389 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 448
           ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN
Sbjct: 361 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 420

Query: 449 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 508
           KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS
Sbjct: 421 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 480

Query: 509 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTLL 568
           NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTLL
Sbjct: 481 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTLL 539

BLAST of CsaV3_6G003270 vs. NCBI nr
Match: KAA0042711.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 953.0 bits (2462), Expect = 1.2e-273
Identity = 459/536 (85.63%), Postives = 493/536 (91.98%), Query Frame = 0

Query: 31  RTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGL 90
           RT   ILL+ALV VFS  S  SLPLST GRWIIDSQ+G+RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 91  NHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFV 150
           NHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 151 LNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLS 210
           LNKTIAEAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 211 LVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDN 270
           LVAQRF NKSTVVGMSLRNE+RG MENANDWN Y+TQGVTTIH IN  VLVIVSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 271 DLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVI 330
           DLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFV+QPLN+ICA IM+ FIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 301

Query: 331 EGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEL 390
           +G NPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE 
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 391 AETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKE 450
            E+FGVLDSNWTQ+KNPNF +KFQLLQTMLQDP SN SFSYVIYH QS QCI+VSNDNKE
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKE 421

Query: 451 IFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNL 510
           IFLT+CST +RWSH ND+TPIKMSSTGLCLKASGEGLEASLSTDC+ +QS+WSAISNS L
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 481

Query: 511 HLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 567
           HL TVT+ GKSLCLQ I+SSNSSK+VTNSCICT  DP CLQDT+SQWFELV TNTL
Sbjct: 482 HLATVTQGGKSLCLQ-IDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of CsaV3_6G003270 vs. NCBI nr
Match: XP_023533776.1 (uncharacterized protein LOC111795530 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 952.6 bits (2461), Expect = 1.5e-273
Identity = 467/538 (86.80%), Postives = 490/538 (91.08%), Query Frame = 0

Query: 29  MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 88
           M RT QV+L LAL  VF S S YSLPLST GRWIIDS +G+RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGRTFQVVLFLALF-VFLSPSVYSLPLSTKGRWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 89  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 148
           GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLE AKAGLAQ NP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEPAKAGLAQNNP 120

Query: 149 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 208
           FVLNKTIAEAYEAVVDVLG SGLMVIADNH+SQPRWCCSLDDGNGFFG+RYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 209 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 268
           LSLVAQRF+ KSTVVGMSLRNE+RG  ENANDWNNYVTQGVTTIH INP VLVIVSGLNY
Sbjct: 181 LSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNY 240

Query: 269 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 328
           DNDLRCLK+KPLNVSTLDNKL FEVHLYSFSGDSESKF+ QPLNNICA I++ F+DHAEF
Sbjct: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDSESKFINQPLNNICANIINGFVDHAEF 300

Query: 329 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 388
           V EG NPFPLFVSEYGYDQREV+DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 389 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 448
           E  E FGVLDSNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQCI+VSNDN
Sbjct: 361 EPGEAFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDN 420

Query: 449 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 508
           K+IFL NCS SSRW+HDNDSTPI+MSSTGLCLK SGEGL  SLSTDC+G Q+ WSAISN+
Sbjct: 421 KDIFLGNCSISSRWTHDNDSTPIRMSSTGLCLKTSGEGLMPSLSTDCLGPQNSWSAISNT 480

Query: 509 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 567
            LHL T+  D KSLCLQ IESSNSSKIVTNS ICT   P CL+DT+SQWFELV TNTL
Sbjct: 481 KLHLATIAPDEKSLCLQ-IESSNSSKIVTNSFICTNGAPNCLEDTRSQWFELVKTNTL 536

BLAST of CsaV3_6G003270 vs. NCBI nr
Match: KAA0042713.1 (major extracellular endoglucanase-like [Cucumis melo var. makuwa])

HSP 1 Score: 949.9 bits (2454), Expect = 1.0e-272
Identity = 458/536 (85.45%), Postives = 492/536 (91.79%), Query Frame = 0

Query: 31  RTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGL 90
           RT   ILLLALV VFS  S  SLPLST GRWIID Q+G+RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 91  NHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFV 150
           NHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 151 LNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLS 210
           LNKTIAEAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 211 LVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDN 270
           LVAQRF NKSTVVGMSLRNE+RG MENANDWN Y+TQGVTTIH IN  VLVIVSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 271 DLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVI 330
           DLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFV+QPLNNICA IM+ FIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVM 301

Query: 331 EGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEL 390
           +G NPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE 
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 391 AETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKE 450
            E+FGVLDSNWTQ+KNPNF +KFQLLQTMLQDP SNASFSYVIYH Q+ QCI+VSNDNKE
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKE 421

Query: 451 IFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNL 510
           IFLT+CST +RWSH ND+TPIKMSSTGLCLKASGEGLEASLSTDC+ +QS+WSAISNS L
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKL 481

Query: 511 HLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 567
           HL TV + G SLCLQ I+SSNSSK+VTNSCICT  DP CLQDT+SQWFELVATNTL
Sbjct: 482 HLATVAQGGNSLCLQ-IDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of CsaV3_6G003270 vs. ExPASy Swiss-Prot
Match: C0HLA0 (Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 424.1 bits (1089), Expect = 2.5e-117
Identity = 232/548 (42.34%), Postives = 328/548 (59.85%), Query Frame = 0

Query: 37  LLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLK 96
           LL AL+ +  +  ++SLPL T GRWI+D  +G RVKL CVNW  H +  L EGLN  P+ 
Sbjct: 13  LLTALLLLLVAAPSHSLPLLTRGRWIVDEATGLRVKLACVNWVGHLEPGLPEGLNRLPVA 72

Query: 97  ELADEAIKLRFNCVRLTYATHMFTR--YANRTVEENFDLLDLEQAKAGLAQYNPFVLNKT 156
            +A     L FNCVRLTY+ HM TR  Y N TV + F  L+L +A +G+   NP +L+  
Sbjct: 73  TVAHTISSLGFNCVRLTYSIHMLTRTSYTNATVAQTFARLNLTEAASGIEHNNPELLDLG 132

Query: 157 IAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQ 216
              AY  VV  L  +G+MVI DNH+S+P+WCC++DDGNGFFG+RYF+P  W++GL L+A 
Sbjct: 133 HVAAYHHVVAALSEAGVMVILDNHVSKPKWCCAVDDGNGFFGDRYFNPNTWVEGLGLMAT 192

Query: 217 RFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRC 276
            FNN   VV MSLRNELRG       W+ ++  G  T+HK NP VLVI+SGL +D DL  
Sbjct: 193 YFNNTPNVVAMSLRNELRGNRSTPISWSRHMQWGAATVHKANPKVLVILSGLQFDTDLSF 252

Query: 277 LKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPN 336
           L   P+ +     K+ +E H YSF     +       N++C      F  +  FV    N
Sbjct: 253 LPVLPVTL-PFKEKIVYEGHWYSFGVPWRTGLP----NDVCKNETGRFKSNVGFVTSSAN 312

Query: 337 --PFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ---AE 396
               PLF+SE+G DQR V+D +NR+++C  A+LA++DLDWALWT  GSYYYR  +    +
Sbjct: 313 ATAAPLFMSEFGIDQRYVNDNDNRYLNCILAYLAEEDLDWALWTMGGSYYYRSDKQPVKD 372

Query: 397 LAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPY-SNASFSYVIYHVQSGQCIEVSNDN 456
             ET+G  + +W++I+NP+F+ + + +Q  +QDPY +   +  +IYH  SG C+E S   
Sbjct: 373 FEETYGFFNHDWSRIRNPDFISRLKEIQQPIQDPYLAPGPYYQIIYHPASGLCVE-SGIG 432

Query: 457 KEIFLTNC-STSSRWSHD-NDSTPIKMSSTGLCLKASGEGLEASLSTDCIG-KQSLWSAI 516
             + L +C S  SRW++D +   PI +  +  C+   G GL A ++ +C     +LWS +
Sbjct: 433 NTVHLGSCQSVRSRWNYDASVKGPIGLMGSSSCISTQGNGLPAIMTENCSAPNNTLWSTV 492

Query: 517 SNSNLHLGT--VTEDGKS--LCLQIIESSNSSKIVTNSCICTTNDPTCLQ--DTQSQWFE 568
           S++ L LGT  + +DGK   +CL   + S S  I TN CIC T D  C    + + QWF+
Sbjct: 493 SSAQLQLGTRVLGKDGKEKWMCL---DGSKSPLISTNECICIT-DSHCYPKLNPEKQWFK 550

BLAST of CsaV3_6G003270 vs. ExPASy Swiss-Prot
Match: P19487 (Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (strain ATCC 33913 / DSM 3586 / NCPPB 528 / LMG 568 / P 25) OX=190485 GN=engXCA PE=1 SV=2)

HSP 1 Score: 83.6 bits (205), Expect = 8.0e-15
Identity = 101/406 (24.88%), Postives = 161/406 (39.66%), Query Frame = 0

Query: 37  LLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVN-WPSHTQSMLIEGLNHRPL 96
           L LA     ++  A+S  ++ + R I+D  SGK V+L  VN +   T + ++ GL  R  
Sbjct: 10  LALATALALAAGPAFSYSIN-NSRQIVD-DSGKVVQLKGVNVFGFETGNHVMHGLWARNW 69

Query: 97  KELADEAIKLRFNCVRLTYATHMF---TRYANRTVEENFDLLDLEQAKAGLAQYNPFVLN 156
           K++  +   L FN VRL +        T  A+     N DL  L   +         +L+
Sbjct: 70  KDMIVQMQGLGFNAVRLPFCPATLRSDTMPASIDYSRNADLQGLTSLQ---------ILD 129

Query: 157 KTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLV 216
           K IAE          A G+ V+ D+H      C  + +   ++   Y + Q WL  L  V
Sbjct: 130 KVIAE--------FNARGMYVLLDHHTPD---CAGISE--LWYTGSYTEAQ-WLADLRFV 189

Query: 217 AQRFNNKSTVVGMSLRNELRGMM-----ENANDWNNYVTQGVTTIHKINPAVLVIVSGLN 276
           A R+ N   V+G+ L+NE  G         A DWN    +G   +  + P  L+ V G+ 
Sbjct: 190 ANRYKNVPYVLGLDLKNEPHGAATWGTGNAATDWNKAAERGSAAVLAVAPKWLIAVEGIT 249

Query: 277 ------------YDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPL-NNI 336
                       +  +L+ L   PLN+    N+L    H+Y      +S F      NN+
Sbjct: 250 DNPVCSTNGGIFWGGNLQPLACTPLNIPA--NRLLLAPHVYGPDVFVQSYFNDSNFPNNM 309

Query: 337 CAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWAL 396
            A     F   A           L + E+G    E D  +  +      +L  K ++   
Sbjct: 310 PAIWERHFGQFA-------GTHALLLGEFGGKYGEGDARDKTWQDALVKYLRSKGINQGF 368

Query: 397 -WTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFVQKFQLLQTM 420
            W+W              +T G+L  +WT ++      K  LL+T+
Sbjct: 370 YWSW---------NPNSGDTGGILRDDWTSVRQ----DKMTLLRTL 368

BLAST of CsaV3_6G003270 vs. ExPASy Swiss-Prot
Match: P23548 (Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2)

HSP 1 Score: 82.0 bits (201), Expect = 2.3e-14
Identity = 80/374 (21.39%), Postives = 148/374 (39.57%), Query Frame = 0

Query: 57  THGRWIIDSQSGKRVKLVCVNW-PSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLTYA 116
           T G  I+D +SGK      +NW    T +  + GL  R + ++ D+  K  +N +RL Y+
Sbjct: 41  TQGNKIVD-ESGKEAAFNGLNWFGLETPNYTLHGLWSRSMDDMLDQVKKEGYNLIRLPYS 100

Query: 117 THMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASGLMVIA 176
             +F   +        D +D  +        NP ++     +  + +++  G  G+ +I 
Sbjct: 101 NQLFDSSSRP------DSIDYHK--------NPDLVGLNPIQIMDKLIEKAGQRGIQIIL 160

Query: 177 DNHMSQPRWCCSLDDGNGFFGNRYFDPQ----EWLQGLSLVAQRFNNKSTVVGMSLRNEL 236
           D H            G+G     ++  Q     W+    ++A R+ N  TV+G  L NE 
Sbjct: 161 DRHR----------PGSGGQSELWYTSQYPESRWISDWKMLADRYKNNPTVIGADLHNEP 220

Query: 237 RGMME----NAN-DWNNYVTQGVTTIHKINPAVLVIVSGLNYD-----------NDLRCL 296
            G       NA+ DW     +    I  +NP  L++V G++++            +L  +
Sbjct: 221 HGQASWGTGNASTDWRLAAQRAGNAILSVNPNWLILVEGVDHNVQGNNSQYWWGGNLTGV 280

Query: 297 KDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPL--NNICAKIMHEFIDHAEFVIEGP 356
            + P+ V  + N++ +  H Y   G S   +   P   +N+ A       D     I   
Sbjct: 281 ANYPV-VLDVPNRVVYSPHDYG-PGVSSQPWFNDPAFPSNLPA-----IWDQTWGYISKQ 340

Query: 357 NPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAET 408
           N  P+ V E+G    ++   E ++ +    ++   +L +  W+               +T
Sbjct: 341 NIAPVLVGEFGGRNVDLSCPEGKWQNALVHYIGANNLYFTYWSL---------NPNSGDT 373

BLAST of CsaV3_6G003270 vs. ExPASy TrEMBL
Match: A0A0A0K853 (Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3 SV=1)

HSP 1 Score: 1103.2 bits (2852), Expect = 0.0e+00
Identity = 539/539 (100.00%), Postives = 539/539 (100.00%), Query Frame = 0

Query: 29  MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 88
           MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE
Sbjct: 1   MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 60

Query: 89  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 148
           GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 120

Query: 149 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 208
           FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 180

Query: 209 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 268
           LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY
Sbjct: 181 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 240

Query: 269 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 328
           DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF
Sbjct: 241 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 300

Query: 329 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 388
           VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 389 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 448
           ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN
Sbjct: 361 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 420

Query: 449 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 508
           KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS
Sbjct: 421 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 480

Query: 509 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTLL 568
           NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTLL
Sbjct: 481 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTLL 539

BLAST of CsaV3_6G003270 vs. ExPASy TrEMBL
Match: A0A5A7TMC9 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002090 PE=3 SV=1)

HSP 1 Score: 953.0 bits (2462), Expect = 5.7e-274
Identity = 459/536 (85.63%), Postives = 493/536 (91.98%), Query Frame = 0

Query: 31  RTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGL 90
           RT   ILL+ALV VFS  S  SLPLST GRWIIDSQ+G+RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLMALVFVFSPLSTNSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 91  NHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFV 150
           NHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 151 LNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLS 210
           LNKTIAEAYEAVVDVLGASGLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDVLGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 211 LVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDN 270
           LVAQRF NKSTVVGMSLRNE+RG MENANDWN Y+TQGVTTIH IN  VLVIVSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 271 DLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVI 330
           DLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFV+QPLN+ICA IM+ FIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHAGFVM 301

Query: 331 EGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEL 390
           +G NPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE 
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 391 AETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKE 450
            E+FGVLDSNWTQ+KNPNF +KFQLLQTMLQDP SN SFSYVIYH QS QCI+VSNDNKE
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNESFSYVIYHPQSSQCIQVSNDNKE 421

Query: 451 IFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNL 510
           IFLT+CST +RWSH ND+TPIKMSSTGLCLKASGEGLEASLSTDC+ +QS+WSAISNS L
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAISNSKL 481

Query: 511 HLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 567
           HL TVT+ GKSLCLQ I+SSNSSK+VTNSCICT  DP CLQDT+SQWFELV TNTL
Sbjct: 482 HLATVTQGGKSLCLQ-IDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNTL 536

BLAST of CsaV3_6G003270 vs. ExPASy TrEMBL
Match: A0A5A7THG1 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002110 PE=3 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.9e-273
Identity = 458/536 (85.45%), Postives = 492/536 (91.79%), Query Frame = 0

Query: 31  RTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGL 90
           RT   ILLLALV VFS  S  SLPLST GRWIID Q+G+RVKLVC+NWPSHTQSMLIEGL
Sbjct: 2   RTFLTILLLALVFVFSPLSTNSLPLSTRGRWIIDLQTGRRVKLVCMNWPSHTQSMLIEGL 61

Query: 91  NHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNPFV 150
           NHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLAQYNPFV
Sbjct: 62  NHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLAQYNPFV 121

Query: 151 LNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLS 210
           LNKTIAEAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWLQGLS
Sbjct: 122 LNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWLQGLS 181

Query: 211 LVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDN 270
           LVAQRF NKSTVVGMSLRNE+RG MENANDWN Y+TQGVTTIH IN  VLVIVSGLNYDN
Sbjct: 182 LVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGLNYDN 241

Query: 271 DLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVI 330
           DLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFV+QPLNNICA IM+ FIDHA FV+
Sbjct: 242 DLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNNICANIMNGFIDHAGFVM 301

Query: 331 EGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAEL 390
           +G NPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REGQAE 
Sbjct: 302 QGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREGQAEP 361

Query: 391 AETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDNKE 450
            E+FGVLDSNWTQ+KNPNF +KFQLLQTMLQDP SNASFSYVIYH Q+ QCI+VSNDNKE
Sbjct: 362 GESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQTSQCIQVSNDNKE 421

Query: 451 IFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNSNL 510
           IFLT+CST +RWSH ND+TPIKMSSTGLCLKASGEGLEASLSTDC+ +QS+WSAISNS L
Sbjct: 422 IFLTDCSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLIQQSVWSAISNSKL 481

Query: 511 HLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 567
           HL TV + G SLCLQ I+SSNSSK+VTNSCICT  DP CLQDT+SQWFELVATNTL
Sbjct: 482 HLATVAQGGNSLCLQ-IDSSNSSKVVTNSCICTNGDPNCLQDTRSQWFELVATNTL 536

BLAST of CsaV3_6G003270 vs. ExPASy TrEMBL
Match: A0A5A7TMX0 (Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold44G002070 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.4e-272
Identity = 461/541 (85.21%), Postives = 496/541 (91.68%), Query Frame = 0

Query: 27  EKMERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSML 86
           EKM RT  VILLLALV VFS   A SLPLST GRWIIDSQ+G+RVKLVC+NWPSHTQSML
Sbjct: 2   EKM-RTFLVILLLALVFVFSPLFANSLPLSTRGRWIIDSQTGRRVKLVCMNWPSHTQSML 61

Query: 87  IEGLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQY 146
           IEGLNHRPLK+LADEAIKLRFNCVRLTYATHMFTRYANRT+EENFDLLDL+QAKAGLA Y
Sbjct: 62  IEGLNHRPLKDLADEAIKLRFNCVRLTYATHMFTRYANRTIEENFDLLDLKQAKAGLALY 121

Query: 147 NPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWL 206
           NPFVLNKTIAEAYEAVVD+LGASGLMVIADNH+SQPRWCCSLDDGNGFFGN  FDPQEWL
Sbjct: 122 NPFVLNKTIAEAYEAVVDILGASGLMVIADNHISQPRWCCSLDDGNGFFGNNNFDPQEWL 181

Query: 207 QGLSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGL 266
           QGLSLVAQRF NKSTVVGMSLRNE+RG MENANDWN Y+TQGVTTIH IN  VLVIVSGL
Sbjct: 182 QGLSLVAQRFRNKSTVVGMSLRNEIRGFMENANDWNKYITQGVTTIHNINSEVLVIVSGL 241

Query: 267 NYDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHA 326
           NYDNDLRCLK+KPLNV TLDNKL FEVHLYSFSGDSESKFV+QPLN+ICA IM+ FIDHA
Sbjct: 242 NYDNDLRCLKEKPLNVGTLDNKLVFEVHLYSFSGDSESKFVKQPLNDICANIMNGFIDHA 301

Query: 327 EFVIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREG 386
            FV++G NPFPLFVSE+GYDQREV+DAENRFMSCFTAHLA +DLDWALW WQGSYY+REG
Sbjct: 302 GFVMQGSNPFPLFVSEFGYDQREVNDAENRFMSCFTAHLALRDLDWALWAWQGSYYFREG 361

Query: 387 QAELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSN 446
           QAE  E+FGVLDSNWTQ+KNPNF +KFQLLQTMLQDP SNASFSYVIYH QS QCI+VSN
Sbjct: 362 QAEPGESFGVLDSNWTQVKNPNFAKKFQLLQTMLQDPNSNASFSYVIYHPQSSQCIQVSN 421

Query: 447 DNKEIFLTNC-STSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAI 506
           DNKEIFLT+C ST +RWSH ND+TPIKMSSTGLCLKASGEGLEASLSTDC+ +QS+WSAI
Sbjct: 422 DNKEIFLTDCSSTPTRWSHINDNTPIKMSSTGLCLKASGEGLEASLSTDCLSQQSVWSAI 481

Query: 507 SNSNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNT 566
           SNS LHL T+T+ GKSLCLQ I+SSN SK+VTNSCICT  DP CLQDT+SQWFELV TNT
Sbjct: 482 SNSKLHLATITQGGKSLCLQ-IDSSNPSKVVTNSCICTNGDPNCLQDTRSQWFELVGTNT 540

BLAST of CsaV3_6G003270 vs. ExPASy TrEMBL
Match: A0A6J1JZU8 (Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=3 SV=1)

HSP 1 Score: 947.2 bits (2447), Expect = 3.1e-272
Identity = 462/538 (85.87%), Postives = 488/538 (90.71%), Query Frame = 0

Query: 29  MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 88
           M R  QV+  LAL  VF S   YSLPLST  +WIIDS +G+RVKLVCVNWPSHTQSMLIE
Sbjct: 1   MGRIFQVVFFLALF-VFLSPYVYSLPLSTKEKWIIDSTTGRRVKLVCVNWPSHTQSMLIE 60

Query: 89  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTRYANRTVEENFDLLDLEQAKAGLAQYNP 148
           GLNHRPLKELADEAIKLRFNCVRLTYAT MFTRYANRTVEENFDLLDLEQAKAGLAQ NP
Sbjct: 61  GLNHRPLKELADEAIKLRFNCVRLTYATQMFTRYANRTVEENFDLLDLEQAKAGLAQNNP 120

Query: 149 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 208
           FVLNKTIAEAYEAVVDVLG SGLMVIADNH+SQPRWCCSLDDGNGFFG+RYFDPQEWLQG
Sbjct: 121 FVLNKTIAEAYEAVVDVLGESGLMVIADNHISQPRWCCSLDDGNGFFGDRYFDPQEWLQG 180

Query: 209 LSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNY 268
           LSLVAQRF+ KSTVVGMSLRNE+RG  ENANDWNNYVTQGVTTIH INP VLVIVSGLNY
Sbjct: 181 LSLVAQRFSKKSTVVGMSLRNEIRGTNENANDWNNYVTQGVTTIHNINPNVLVIVSGLNY 240

Query: 269 DNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEF 328
           DNDLRCLK+KPLNVSTLDNKL FEVHLYSFSGD ESKF+ QPLNNICA I++ F+DHAEF
Sbjct: 241 DNDLRCLKEKPLNVSTLDNKLVFEVHLYSFSGDPESKFINQPLNNICANIINGFVDHAEF 300

Query: 329 VIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 388
           V EG NPFPLFVSEYGYDQREV+DAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA
Sbjct: 301 VTEGSNPFPLFVSEYGYDQREVNDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQA 360

Query: 389 ELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSNDN 448
           E  ETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDP SNASFSYVIYH QSGQCI+VSNDN
Sbjct: 361 EPGETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPNSNASFSYVIYHPQSGQCIQVSNDN 420

Query: 449 KEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQSLWSAISNS 508
           K+IF+ NCS SSRW+HDNDSTPI+MSS GLCLK  GEGL  SLSTDC+G QS WSAISN+
Sbjct: 421 KDIFMGNCSISSRWTHDNDSTPIRMSSMGLCLKTIGEGLTPSLSTDCLGPQSSWSAISNT 480

Query: 509 NLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVATNTL 567
            LHL T+++DGKSLCLQ +ESSNSSKIVTNSCICT   P CLQDT+SQWFELV TNTL
Sbjct: 481 KLHLATISQDGKSLCLQ-VESSNSSKIVTNSCICTNGAPNCLQDTRSQWFELVKTNTL 536

BLAST of CsaV3_6G003270 vs. TAIR 10
Match: AT1G13130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 410.6 bits (1054), Expect = 2.0e-114
Identity = 211/515 (40.97%), Postives = 314/515 (60.97%), Query Frame = 0

Query: 52  SLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVR 111
           S PLST  RWI+D ++G RVKLVC NWPSH Q ++ EGL+ +P+  +A + +++ FNCVR
Sbjct: 32  SYPLSTSSRWIVD-ENGLRVKLVCANWPSHLQPVVAEGLSKQPVDAVAKKIVEMGFNCVR 91

Query: 112 LTYATHMFTRYA---NRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGA 171
           LT+   + T      N TV ++F  L L     G    NP +++  + EAY+ VV  LG 
Sbjct: 92  LTWPLDLMTNETLANNVTVRQSFQSLGLNDDIVGFQTNNPSIIDLPLIEAYKTVVTTLGN 151

Query: 172 SGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLR 231
           + +MVI DNH+++P WCC+ DDGNGFFG+++FDP  W+  L  +A  FN  S VVGMSLR
Sbjct: 152 NDVMVILDNHLTKPGWCCANDDGNGFFGDQFFDPTVWVAALKKMAATFNGVSNVVGMSLR 211

Query: 232 NELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNK 291
           NELRG  +N NDW  Y+ QG   +H  N  VLVI+SGL++D DL  ++ +P+ +S    K
Sbjct: 212 NELRGPKQNVNDWFKYMQQGAEAVHSANNKVLVILSGLSFDADLSFVRSRPVKLS-FTGK 271

Query: 292 LAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQR 351
           L FE+H YSFS D  S     P N+IC ++++   +   +++     FPLF+SE+G D+R
Sbjct: 272 LVFELHWYSFS-DGNSWAANNP-NDICGRVLNRIGNGGGYLLN--QGFPLFLSEFGIDER 331

Query: 352 EVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPN 411
            V+  +NR+  C T   A+ D+DW+LW   GSYY R+G+  + E +GVLDS+W  ++N +
Sbjct: 332 GVNTNDNRYFGCLTGWAAENDVDWSLWALTGSYYLRQGKVGMNEYYGVLDSDWISVRNSS 391

Query: 412 FVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVS-NDNKEIFLTNCSTSSRWSHDND 471
           F+QK   LQ+ LQ P        +++H  +G CI  S +D K + L  C++S  WS+   
Sbjct: 392 FLQKISFLQSPLQGPGPRTDAYNLVFHPLTGLCIVRSLDDPKMLTLGPCNSSEPWSYTKK 451

Query: 472 STPIKMSSTGLCLKASGEGLEASLS-TDCIGKQSLWSAISNSNLHLGTVTEDGKSLCLQI 531
           +  IK     LCL+++G     +++ T C    S W  IS S +HL + T +  SLCL +
Sbjct: 452 ALRIK--DQQLCLQSNGPKNPVTMTRTSCSTSGSKWQTISASRMHLASTTSNKTSLCLDV 511

Query: 532 IESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELV 562
             ++N   +V N+C C + D +C  +  SQWF+++
Sbjct: 512 DTANN---VVANACKCLSKDKSC--EPMSQWFKII 533

BLAST of CsaV3_6G003270 vs. TAIR 10
Match: AT3G26140.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 377.9 bits (969), Expect = 1.5e-104
Identity = 200/515 (38.83%), Postives = 311/515 (60.39%), Query Frame = 0

Query: 54  PLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPLKELADEAIKLRFNCVRLT 113
           PLST+ RWIID + G+RVKL CVNWPSH Q ++ EGL+ + + +LA + + + FNCVR T
Sbjct: 4   PLSTNSRWIID-EKGQRVKLACVNWPSHLQPVVAEGLSKQSVDDLAKKIMAMGFNCVRFT 63

Query: 114 YATHMFTRYA---NRTVEENFDLLDLEQAKAGLAQYNPFVLNKTIAEAYEAVVDVLGASG 173
           +   + T      N TV ++F  L L    +G    NP +++  + EAY+ VV  LG + 
Sbjct: 64  WPLDLATNETLANNVTVRQSFQSLGLNDDISGFETKNPSMIDLPLIEAYKKVVAKLGNNN 123

Query: 174 LMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLVAQRFNNKSTVVGMSLRNE 233
           +MVI DNH+++P WCC  +DGNGFFG+ +FDP  W+ GL+ +A  F   + VVGMSLRNE
Sbjct: 124 VMVILDNHVTKPGWCCGYNDGNGFFGDTFFDPTTWIAGLTKIAMTFKGATNVVGMSLRNE 183

Query: 234 LRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDNDLRCLKDKPLNVSTLDNKLA 293
           LRG  +N +DW  Y+ QG   +H+ NP VLVI+SGL+YD DL  ++ + +N+ T   KL 
Sbjct: 184 LRGPKQNVDDWFKYMQQGAEAVHEANPNVLVILSGLSYDTDLSFVRSRHVNL-TFTRKLV 243

Query: 294 FEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIEGPNPFPLFVSEYGYDQREV 353
           FE+H YSF+  + + +  +  N  C +I+    +   F +     FP+F+SE+G D R  
Sbjct: 244 FELHRYSFT--NTNTWSSKNPNEACGEILKSIENGGGFNL---RDFPVFLSEFGIDLRGK 303

Query: 354 DDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAETFGVLDSNWTQIKNPNFV 413
           +  +NR++ C     A+ D+DW++WT QGSYY REG   ++E +G+LDS+W ++++ +F+
Sbjct: 304 NVNDNRYIGCILGWAAENDVDWSIWTLQGSYYLREGVVGMSEFYGILDSDWVRVRSQSFL 363

Query: 414 QKFQLLQTMLQDPYSNASFSYVIYHVQSGQC-IEVSNDNKEIFLTNCSTSSRWSHDNDST 473
           Q+  L+ + LQ P S +    +++H  +G C ++   D  ++ L  C+ S  WS+   +T
Sbjct: 364 QRLSLILSPLQGPGSQSKVYNLVFHPLTGLCMLQSILDPTKVTLGLCNESQPWSYTPQNT 423

Query: 474 PIKMSSTGLCLKASGEGLEASLS-TDCIGKQ-SLWSAISNSNLHLGTVTEDGKSLCLQII 533
            + +    LCL+++G      LS T C     S W  IS SN+ L   + +  SLCL + 
Sbjct: 424 -LTLKDKSLCLESTGPNAPVKLSETSCSSPNLSEWETISASNMLLAAKSTN-NSLCLDVD 483

Query: 534 ESSNSSKIVTNSCICTT-NDPTCLQDTQSQWFELV 562
           E++N   ++ ++C C    D +C  D  SQWF++V
Sbjct: 484 ETNN---LMASNCKCVKGEDSSC--DPISQWFKIV 504

BLAST of CsaV3_6G003270 vs. TAIR 10
Match: AT3G26130.1 (Cellulase (glycosyl hydrolase family 5) protein )

HSP 1 Score: 377.1 bits (967), Expect = 2.5e-104
Identity = 214/537 (39.85%), Postives = 307/537 (57.17%), Query Frame = 0

Query: 33  IQVILLLALVSVFSSFSAYSLPLSTHGRWII-DSQSGKRVKLVCVNWPSHTQSMLIEGLN 92
           I V LL  +++ F      + P ST  RWI+ D   G+RVKL CVNWPSH ++ + EGL+
Sbjct: 7   ISVFLLPYVITTF------AFPPSTDSRWIVDDGNKGRRVKLTCVNWPSHLETAVAEGLS 66

Query: 93  HRPLKELADEAIKLRFNCVRLTYATHMFTR---YANRTVEENFDLLDLEQAKAGLAQYNP 152
            +PL  +A++ + + FNCVRLT+  ++ T     A  TV ++     L +A +G   +NP
Sbjct: 67  KQPLDAIAEKIVSMGFNCVRLTWPLYLATDESFSAFMTVRQSLRKFRLFEAVSGFQTHNP 126

Query: 153 FVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQG 212
            +L+  + +A++ VV  L    +MVI DNH+SQP WCCS +DGNGFFG+++ +PQ W++G
Sbjct: 127 TILDLPLIKAFQEVVYCLEKHRVMVILDNHISQPGWCCSDNDGNGFFGDKHLNPQVWIKG 186

Query: 213 LSLVAQRFNN-KSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLN 272
           L  +A  F N  S VVGMSLRNELRG  +N  DW  Y+ +G   +H +NP VLVIVSGLN
Sbjct: 187 LKKMASMFANVSSNVVGMSLRNELRGPKQNIKDWYKYMREGAEAVHSVNPNVLVIVSGLN 246

Query: 273 YDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAE 332
           Y  DL  L+++P  VS    K+ FE+H Y F    E       LN IC K   + +  + 
Sbjct: 247 YATDLSFLRERPFEVS-FRRKVVFEIHWYGFWNTWEG----DNLNKICGKETEKMMKMSG 306

Query: 333 FVIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQ 392
           F++E     PLFVSE+G DQR  +  +N+F+SCF A  A +DLDW+LWT  GSYY RE  
Sbjct: 307 FLLE--KGIPLFVSEFGIDQRGNNANDNKFLSCFMALAADRDLDWSLWTLAGSYYIREKS 366

Query: 393 AELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSND 452
               E++GVLD NW+ I+N   +Q    +QT             +++H  +G CI V   
Sbjct: 367 IGSDESYGVLDFNWSSIRNSTILQMISAIQTPFIG-LMETQPKKIMFHPSTGLCI-VRKS 426

Query: 453 NKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKASGEGLEASLSTDCIGKQ-SLWSAIS 512
             ++ L +C+ S  W   +           LCLKA  +G    L         S W   S
Sbjct: 427 LFQLKLGSCNRSESWRLSSHRVLSLAEEQILCLKAYEKGKSVKLRLFFSESYCSKWKLFS 486

Query: 513 NSNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFELVAT 564
           +S + L ++T++G S+CL +   +N+  IVTNSC C   + +C  D +SQWF+LV +
Sbjct: 487 DSKMQLSSITKNGFSVCLDVDTENNN--IVTNSCKCLRGNSSC--DPRSQWFKLVTS 524

BLAST of CsaV3_6G003270 vs. TAIR 10
Match: AT5G17500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 354.8 bits (909), Expect = 1.3e-97
Identity = 195/531 (36.72%), Postives = 295/531 (55.56%), Query Frame = 0

Query: 36  ILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIEGLNHRPL 95
           + L   +S+ S   A   PL T  RWI++++ G RVKL C NWPSH + ++ EGL+ +P+
Sbjct: 9   VFLFLFLSLISLTLATDYPLFTKSRWIVNNK-GHRVKLACANWPSHLKPVVAEGLSSQPM 68

Query: 96  KELADEAIKLRFNCVRLTYATHMF---TRYANRTVEENFDLLDLEQAKAGLAQYNPFVLN 155
             ++ +   + FNCVRLT+   +    T   N TV+++F+   L+    G+  +NP+++N
Sbjct: 69  DSISKKIKDMGFNCVRLTWPLELMINDTLAFNVTVKQSFERYGLDHELQGIYTHNPYIVN 128

Query: 156 KTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEWLQGLSLV 215
             +   ++AVV  LG   +MVI DNH + P WCCS DD + FFG+  F+P  W+ GL  +
Sbjct: 129 TPLINVFQAVVYSLGRHDVMVILDNHKTVPGWCCSNDDPDAFFGDPKFNPDLWMLGLKKM 188

Query: 216 AQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSGLNYDNDL 275
           A  F N   VVGMSLRNELRG    + DW  Y+ +G   +H  NP VLVI+SGLN+D DL
Sbjct: 189 ATIFMNVKNVVGMSLRNELRGYNHTSKDWYKYMQKGAEAVHTSNPNVLVILSGLNFDADL 248

Query: 276 RCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDHAEFVIEG 335
             LKD+P+N+S    KL  E+H YSF+ D   ++    +N+ C+++  +      FV++ 
Sbjct: 249 SFLKDRPVNLS-FKKKLVLELHWYSFT-DGTGQWKSHNVNDFCSQMFSKERRTGGFVLD- 308

Query: 336 PNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYREGQAELAE 395
              FPLF+SE+G DQR  D   NR+M+C  A  A+KDLDWA+W   G YY+REG+  + E
Sbjct: 309 -QGFPLFLSEFGTDQRGGDLEGNRYMNCMLAWAAEKDLDWAVWAVTGVYYFREGKRGVVE 368

Query: 396 TFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCIEVSN--DNKE 455
            +G+LD+NW  + N  ++++  ++Q     P    +    I+H  +G C+   +     E
Sbjct: 369 AYGMLDANWHNVHNYTYLRRLSVIQPPHTGPGVKHNHHKKIFHPLTGLCLVRKSHCHESE 428

Query: 456 IFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKA-SGEGLEASLSTDCIGKQSLWSAISNSN 515
           + L  C+    WS+ +            CL+  +  G    L   C   +     IS + 
Sbjct: 429 LTLGPCTKDEPWSYSHGGILEIRRGHKSCLEGETAVGKSVKLGRICTKIEQ----ISATK 488

Query: 516 LHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFEL 561
           +HL   T DG  +CL +   +N   +V NSC C T D TC  +  SQWF++
Sbjct: 489 MHLSFNTSDGSLVCLDVDSDNN---VVANSCNCLTGDTTC--EPASQWFKI 525

BLAST of CsaV3_6G003270 vs. TAIR 10
Match: AT5G16700.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 307.8 bits (787), Expect = 1.9e-83
Identity = 187/541 (34.57%), Postives = 286/541 (52.87%), Query Frame = 0

Query: 29  MERTIQVILLLALVSVFSSFSAYSLPLSTHGRWIIDSQSGKRVKLVCVNWPSHTQSMLIE 88
           M R+    L    +S  S  +  S PLST  RWI+D + G+RVKL CVNWP+H Q  + E
Sbjct: 1   MARSFYFCLFFLFISSTSKLTT-SYPLSTKSRWIVD-EKGQRVKLACVNWPAHLQPTVAE 60

Query: 89  GLNHRPLKELADEAIKLRFNCVRLTYATHMFTR---YANRTVEENFDLLDLEQAKAGLAQ 148
           GL+ +PL  ++ + + + FNCVRLT+   + T        TV+++F+ L L +   G+  
Sbjct: 61  GLSKQPLDSISKKIVSMGFNCVRLTWPLDLVTNDTLALKVTVKQSFESLKLFEDVLGIQT 120

Query: 149 YNPFVLNKTIAEAYEAVVDVLGASGLMVIADNHMSQPRWCCSLDDGNGFFGNRYFDPQEW 208
           +NP +L+  +  A++ VV  LG +G+MVI DNH++ P WCC  +D + FFG  +FDP  W
Sbjct: 121 HNPKLLHLPLFNAFQEVVSNLGENGVMVILDNHLTTPGWCCGDNDLDAFFGYPHFDPLVW 180

Query: 209 LQGLSLVAQRFNNKSTVVGMSLRNELRGMMENANDWNNYVTQGVTTIHKINPAVLVIVSG 268
            +GL  +A  F N + V+GMSLRNE RG  +  + W  ++ QG   +H  NP +LVI+SG
Sbjct: 181 AKGLRKMATLFRNFTHVIGMSLRNEPRGARDYPDLWFRHMPQGAEAVHAANPKLLVILSG 240

Query: 269 LNYDNDLRCLKDKPLNVSTLDNKLAFEVHLYSFSGDSESKFVQQPLNNICAKIMHEFIDH 328
           +++D +L  L+D+ +NVS  D KL FE+H YSFS D    + +   N+ C KI+ +   +
Sbjct: 241 IDFDTNLSFLRDRSVNVSFTD-KLVFELHWYSFS-DGRDSWRKHNSNDFCVKIIEKVTHN 300

Query: 329 AEFVIEGPNPFPLFVSEYGYDQREVDDAENRFMSCFTAHLAQKDLDWALWTWQGSYYYRE 388
             F++     FPL +SE+G DQR  D + NR+M+C  A  A+ DLDWA+W   G YY R 
Sbjct: 301 GGFLL--GRGFPLILSEFGTDQRGGDMSGNRYMNCLVAWAAENDLDWAVWALTGDYYLRT 360

Query: 389 GQAELAETFGVLDSNWTQIKNPNFVQKFQLLQTMLQDPYSNASFSYVIYHVQSGQCI--E 448
           G                    PN                       +++H  +G C+   
Sbjct: 361 GPG----------------LRPN---------------------KNLLFHPSTGLCVTNN 420

Query: 449 VSNDNKEIFLTNCSTSSRWSHDNDSTPIKMSSTGLCLKAS---GEGLEASLSTDCIGKQS 508
            S++   + L  C  S  W+ +     + ++   +C++A    G+ ++  + T C    S
Sbjct: 421 PSDNIPTLRLGPCPKSDPWTFNPSEGILWINK--MCVEAPNVVGQKVKLGVGTKC----S 480

Query: 509 LWSAISNSNLHLGTVTEDGKSLCLQIIESSNSSKIVTNSCICTTNDPTCLQDTQSQWFEL 562
               IS + +HL   T +G  LCL + E  NS  +V N C   T D +C  D  SQWF++
Sbjct: 481 KLGQISATKMHLSFKTSNGLLLCLDVDERDNS--VVANRCKFLTMDASC--DPASQWFKV 488

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8646606.10.0e+00100.00hypothetical protein Csa_005432 [Cucumis sativus][more]
XP_011658389.10.0e+00100.00glycosyl hydrolase 5 family protein [Cucumis sativus][more]
KAA0042711.11.2e-27385.63major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
XP_023533776.11.5e-27386.80uncharacterized protein LOC111795530 [Cucurbita pepo subsp. pepo][more]
KAA0042713.11.0e-27285.45major extracellular endoglucanase-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
C0HLA02.5e-11742.34Glycosyl hydrolase 5 family protein OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1[more]
P194878.0e-1524.88Major extracellular endoglucanase OS=Xanthomonas campestris pv. campestris (stra... [more]
P235482.3e-1421.39Endoglucanase OS=Paenibacillus polymyxa OX=1406 PE=3 SV=2[more]
Match NameE-valueIdentityDescription
A0A0A0K8530.0e+00100.00Mannan endo-1,4-beta-mannosidase OS=Cucumis sativus OX=3659 GN=Csa_6G028440 PE=3... [more]
A0A5A7TMC95.7e-27485.63Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7THG14.9e-27385.45Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A5A7TMX02.4e-27285.21Mannan endo-1,4-beta-mannosidase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27... [more]
A0A6J1JZU83.1e-27285.87Mannan endo-1,4-beta-mannosidase OS=Cucurbita maxima OX=3661 GN=LOC111491191 PE=... [more]
Match NameE-valueIdentityDescription
AT1G13130.12.0e-11440.97Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26140.11.5e-10438.83Cellulase (glycosyl hydrolase family 5) protein [more]
AT3G26130.12.5e-10439.85Cellulase (glycosyl hydrolase family 5) protein [more]
AT5G17500.11.3e-9736.72Glycosyl hydrolase superfamily protein [more]
AT5G16700.11.9e-8334.57Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D2.80.10.50coord: 430..560
e-value: 1.3E-5
score: 27.5
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 53..422
e-value: 1.8E-66
score: 226.8
NoneNo IPR availablePANTHERPTHR31263CELLULASE FAMILY PROTEIN (AFU_ORTHOLOGUE AFUA_5G14560)coord: 36..564
IPR001547Glycoside hydrolase, family 5PFAMPF00150Cellulasecoord: 91..378
e-value: 8.0E-26
score: 91.1
IPR035992Ricin B-like lectinsSUPERFAMILY50370Ricin B-like lectinscoord: 428..543
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 54..416

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsaV3_6G003270.1CsaV3_6G003270.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071704 organic substance metabolic process
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds