CsaV3_6G000260 (gene) Cucumber (Chinese Long) v3

BLAST of CsaV3_6G000260 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 732.6 bits (1890), Expect = 1.6e-207
Identity = 355/355 (100.00%), Postives = 355/355 (100.00%), Query Frame = 0

Query: 1   MRDIKIEFIEREISELKTQRMRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAE 60
           MRDIKIEFIEREISELKTQRMRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAE
Sbjct: 1   MRDIKIEFIEREISELKTQRMRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAE 60

Query: 61  DDASYIKEPSFPCFKWRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHL 120
           DDASYIKEPSFPCFKWRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHL
Sbjct: 61  DDASYIKEPSFPCFKWRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHL 120

Query: 121 VGASRTDKGVHAWGQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFS 180
           VGASRTDKGVHAWGQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFS
Sbjct: 121 VGASRTDKGVHAWGQVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFS 180

Query: 181 TLSKVYHYKIYSGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDR 240
           TLSKVYHYKIYSGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDR
Sbjct: 181 TLSKVYHYKIYSGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDR 240

Query: 241 VVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILAS 300
           VVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILAS
Sbjct: 241 VVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILAS 300

Query: 301 RDRRELAKYASGVPPQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 356
           RDRRELAKYASGVPPQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY
Sbjct: 301 RDRRELAKYASGVPPQGLCLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

BLAST of CsaV3_6G000260 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 667.2 bits (1720), Expect = 8.1e-188
Identity = 322/335 (96.12%), Postives = 327/335 (97.61%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60

Query: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200
           TPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 356
           VSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

BLAST of CsaV3_6G000260 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 646.0 bits (1665), Expect = 1.9e-181
Identity = 311/335 (92.84%), Postives = 321/335 (95.82%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRG PNAE D+S I  P FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAE-DSSCIIAPPFPSFKWRLLI 60

Query: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120

Query: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200
           TPFNYDSLESVHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180

Query: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI+MG LV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIDMGPLV 240

Query: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320
           QLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILAS+DRRELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCL 300

Query: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 356
           VSINYKEDHLKLPSDCPGTS GR RSVSKCK+PFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 334

BLAST of CsaV3_6G000260 vs. NCBI nr
Match: TYK02989.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa])

HSP 1 Score: 642.1 bits (1655), Expect = 2.8e-180
Identity = 311/322 (96.58%), Postives = 315/322 (97.83%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60

Query: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200
           TPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 321 VSINYKEDHLKLPSDCPGTSFG 343
           VSINYKEDHLKLPSDCPGTS G
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLG 321

BLAST of CsaV3_6G000260 vs. NCBI nr
Match: XP_038875517.1 (tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 620.5 bits (1599), Expect = 8.7e-174
Identity = 302/335 (90.15%), Postives = 311/335 (92.84%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRG PNAE D+S I  P FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAE-DSSCIIAPPFPSFKWRLLI 60

Query: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120

Query: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200
           TPFNYDSLESVHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIY+G I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQ 180

Query: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVK          G LV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVK----------GPLV 240

Query: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320
           QLEVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILAS+DRRELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCL 300

Query: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 356
           VSINYKEDHLKLPSDCPGTS GR RSVSKCK+PFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 324

BLAST of CsaV3_6G000260 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 214.5 bits (545), Expect = 1.9e-54
Identity = 108/255 (42.35%), Postives = 163/255 (63.92%), Query Frame = 0

Query: 76  WRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQ 135
           ++L IAYDGT Y+GWQ Q +  +IQ  I+EAL     L+++ + LVG+ RTD GVHA GQ
Sbjct: 4   YKLTIAYDGTNYSGWQIQPNASSIQQKIQEAL--CILLKKEKVVLVGSGRTDAGVHAKGQ 63

Query: 136 VAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTI 195
           VAHF    +Y  L  +H +LNGLLP DIR++ + P  P FH+++S + K YHY ++   +
Sbjct: 64  VAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV 123

Query: 196 MDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVV-NPVKVIFRFDVI 255
           MDPFQR Y++H   K++   +K+AA  F G HDF++F N AH      NPV+ ++R D+ 
Sbjct: 124 MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK 183

Query: 256 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 315
                ++LE EG GFLY+ VRN+VG L+ +       E + +I A+++RR+ +  A   P
Sbjct: 184 PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA---P 243

Query: 316 PQGLCLVSINYKEDH 330
           P+GL L+ + Y+ ++
Sbjct: 244 PEGLFLIQVFYENEN 252

BLAST of CsaV3_6G000260 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 189.1 bits (479), Expect = 8.5e-47
Identity = 109/251 (43.43%), Postives = 142/251 (56.57%), Query Frame = 0

Query: 77  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 136
           RL +AYDGT Y G+Q Q     PTIQ  +E  L    +L    L + GASRTD GVHA G
Sbjct: 5   RLTLAYDGTAYHGFQKQSGSGLPTIQETLERCL---AELSGAALKVTGASRTDAGVHARG 64

Query: 137 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 196
           QV +FVT       E + AALNG+LP DI   +      DFHAR+S ++K Y Y +Y+  
Sbjct: 65  QVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHP 124

Query: 197 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 256
           +  PFQR Y+ H    L+  AM+ AA + +G+HDFS F  A     V + V+ + R D+ 
Sbjct: 125 VRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTFQAAGR--PVQSAVRTLTRADIE 184

Query: 257 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 316
               LV+L     GFLY  VR M G L+++G E L PE +P I+AS DR   A+    VP
Sbjct: 185 AQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPTVP 244

Query: 317 PQGLCLVSINY 326
           P GLCL  + Y
Sbjct: 245 PHGLCLEEVEY 247

BLAST of CsaV3_6G000260 vs. ExPASy Swiss-Prot
Match: Q8R7Y7 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=truA PE=3 SV=1)

HSP 1 Score: 186.0 bits (471), Expect = 7.2e-46
Identity = 109/248 (43.95%), Postives = 146/248 (58.87%), Query Frame = 0

Query: 78  LLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVA 137
           L+I YDGT Y GWQYQ++  T+Q ++E+A+    K+  + ++L+G+SRTD GVHA  QVA
Sbjct: 2   LIIEYDGTNYHGWQYQKNAITVQEVLEKAI---EKVTGEKVNLIGSSRTDTGVHALYQVA 61

Query: 138 HFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMD 197
           +F T       E +  ALN +LP D+ V +       FHAR+S  SK Y Y I +     
Sbjct: 62  NFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPM 121

Query: 198 PFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMG 257
           P  R Y +H  Y L+  AMK+AA +F G HDFSAF   A  ++ VNPVK IF   V + G
Sbjct: 122 PTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF--KASGNKKVNPVKTIFDLTVEKEG 181

Query: 258 ALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQG 317
            L+++EVE  GFLY  VR +VG LV +G   +    +P I+ S+DR    K     PPQG
Sbjct: 182 DLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDR---TKAGITAPPQG 240

Query: 318 LCLVSINY 326
           L LV I Y
Sbjct: 242 LYLVRIVY 240

BLAST of CsaV3_6G000260 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 180.6 bits (457), Expect = 3.0e-44
Identity = 103/251 (41.04%), Postives = 145/251 (57.77%), Query Frame = 0

Query: 77  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 136
           +L +AYDGT Y G+Q QR     TIQ  +E+AL   + + +  + ++GA RTD GVHA G
Sbjct: 5   KLTLAYDGTNYHGFQEQRGTGLATIQEALEKAL---STIAQTPIQVIGAGRTDAGVHAQG 64

Query: 137 QVAHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGT 196
           QV +F +       E    ALN LLP DI+V +      DFHARFS ++K Y Y IY   
Sbjct: 65  QVVNFRSEKWPVPAEKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHR 124

Query: 197 IMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVI 256
           +M PF R Y YH   +L+  AM+E A Y +G +DF +F   A    V + ++ I+R D+I
Sbjct: 125 VMSPFHRYYCYHEPRRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADII 184

Query: 257 EMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVP 316
           E   ++ L + G+GFLY  VR + G L+ IG   + PE + KI+ S++R      A   P
Sbjct: 185 EDAPVINLYLRGNGFLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---P 244

Query: 317 PQGLCLVSINY 326
           PQGLCL+ + Y
Sbjct: 245 PQGLCLMEVEY 247

BLAST of CsaV3_6G000260 vs. ExPASy Swiss-Prot
Match: A3DJK8 (tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=truA PE=3 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 2.0e-43
Identity = 98/249 (39.36%), Postives = 144/249 (57.83%), Query Frame = 0

Query: 77  RLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQV 136
           +L+I YDGT Y GWQ Q++  T+Q  IE A+ G T    + + L+GASRTD GVHA GQV
Sbjct: 5   KLIIEYDGTNYHGWQKQKNAKTVQDTIERAIKGLT---GEKVDLIGASRTDFGVHALGQV 64

Query: 137 AHFVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIM 196
           A+F+T       +    ALN +LP DI ++E      DFHAR+    K Y Y IY+    
Sbjct: 65  ANFITNSGIPG-DRFSYALNRMLPDDIVIKESQEVDMDFHARYKAKGKRYRYLIYNSQFP 124

Query: 197 DPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEM 256
               R  TYH  +KL++ +M+ AA+YF+G HDFSAF ++  + +    V+ I    +   
Sbjct: 125 SALLRHRTYHVSHKLDFESMQRAASYFLGTHDFSAFRSSGSSAK--TSVRTITDVSLERE 184

Query: 257 GALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQ 316
             +V+ E+ G GFLY  VR +VG L+++G   +  + +P+I+ SR R+   + A   P +
Sbjct: 185 DKIVKFEIAGDGFLYNMVRIIVGTLMEVGIGKIRADEIPQIIESRKRKRAGRTA---PAE 244

Query: 317 GLCLVSINY 326
           GL LV + Y
Sbjct: 245 GLYLVEVYY 244

BLAST of CsaV3_6G000260 vs. ExPASy TrEMBL
Match: A0A0A0KB67 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.3e-188
Identity = 322/322 (100.00%), Postives = 322/322 (100.00%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80
           MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60

Query: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200
           TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180

Query: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240

Query: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300

Query: 321 VSINYKEDHLKLPSDCPGTSFG 343
           VSINYKEDHLKLPSDCPGTSFG
Sbjct: 301 VSINYKEDHLKLPSDCPGTSFG 322

BLAST of CsaV3_6G000260 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 667.2 bits (1720), Expect = 3.9e-188
Identity = 322/335 (96.12%), Postives = 327/335 (97.61%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60

Query: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200
           TPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 356
           VSINYKEDHLKLPSDCPGTS GR+RSV KCKVPFY
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

BLAST of CsaV3_6G000260 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 642.1 bits (1655), Expect = 1.4e-180
Identity = 311/322 (96.58%), Postives = 315/322 (97.83%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80
           MRCWAVGSPVLPPISLP SPIIPSPNGASSSSSRGFPNAE D+SYI EP FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAE-DSSYITEPPFPSFKWRLLI 60

Query: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200
           TPFNYDSLESVHAALNGLLPVDIRVREMSPA PDFHARFSTLSKVYHYKIY+GTIMDPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQ 180

Query: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHN+RVVNPVKVIFRFDVIEMGALV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALV 240

Query: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320
           QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDR+ELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCL 300

Query: 321 VSINYKEDHLKLPSDCPGTSFG 343
           VSINYKEDHLKLPSDCPGTS G
Sbjct: 301 VSINYKEDHLKLPSDCPGTSLG 321

BLAST of CsaV3_6G000260 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 599.7 bits (1545), Expect = 7.7e-168
Identity = 284/335 (84.78%), Postives = 306/335 (91.34%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80
           M CWAVGS VLP +  PLSP IPS  G  SS+ RGFPN+E D+S + EP FP FKWRL+I
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIPS-RGTLSSTGRGFPNSE-DSSCVPEPDFPSFKWRLVI 60

Query: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140
           AYDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFI 120

Query: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200
           TPFNYDSL+ +HAALNGLLP DIRVRE+SPAVPDFHARFSTLSKVYHYKIY+  I+DPFQ
Sbjct: 121 TPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQ 180

Query: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260
           RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMGAL+
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALI 240

Query: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320
           QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP+VVPKILASRDR+ LAKY + +PPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCL 300

Query: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 356
           V INYKEDHLKLPSDCPGTSFGR+ SVSKCK+PFY
Sbjct: 301 VRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 333

BLAST of CsaV3_6G000260 vs. ExPASy TrEMBL
Match: A0A6J1L2M8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 594.7 bits (1532), Expect = 2.5e-166
Identity = 284/337 (84.27%), Postives = 306/337 (90.80%), Query Frame = 0

Query: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFP--NAEDDASYIKEPSFPCFKWRL 80
           M CWAVGS VLP +  PLSP IPS  G  SS+ RGFP  N+E D+S + EP FP FKWRL
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIPS-RGTLSSTGRGFPVMNSE-DSSCVPEPDFPSFKWRL 60

Query: 81  LIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAH 140
           +IAYDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAH
Sbjct: 61  VIAYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAH 120

Query: 141 FVTPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDP 200
           F+TPFNYDSL+ +HAALNGLLP DIRVRE+SPAVPDFHARFSTLSKVYHYKIY+  I+DP
Sbjct: 121 FITPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDP 180

Query: 201 FQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGA 260
           FQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAF N AHNDRVVNPVKVIFRFDVIEMGA
Sbjct: 181 FQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGA 240

Query: 261 LVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGL 320
           L+QLEVEGSGFLYRQVRNMV LL+QIGKEA+PP+VVPKILASRDR+ LAKY + +PPQGL
Sbjct: 241 LIQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGL 300

Query: 321 CLVSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 356
           CLV INYKEDHLKLPSDCPGTSFGR+ SVSKCK+PFY
Sbjct: 301 CLVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335

BLAST of CsaV3_6G000260 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 430.6 bits (1106), Expect = 1.2e-120
Identity = 213/329 (64.74%), Postives = 250/329 (75.99%), Query Frame = 0

Query: 28  SPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLIAYDGTRY 87
           +P LPP SL  +  + SP   S ++        D  +Y         KWRL+IAYDGTR+
Sbjct: 5   TPALPP-SLFTNGYLSSPQNISMTNLNSSVKVSDSGAY---------KWRLVIAYDGTRF 64

Query: 88  AGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVTPFNYDS 147
           AGWQYQ SPPTIQ ++E+ALI  T+L RK+L L+GA RTD GVHAWGQVAHFVTPFNY S
Sbjct: 65  AGWQYQESPPTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTS 124

Query: 148 LESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQRCYTYHC 207
           L+S HAALNGLLP DIRVRE+S AVP+FHARFS  SKVY Y+IY+ T MDPFQR + YHC
Sbjct: 125 LDSFHAALNGLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHC 184

Query: 208 VYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALVQLEVEGS 267
            YKLN + M+EAAN FVGKHDFSAF NA   D V +P+K I RFDVI+MG+L+QLEVEGS
Sbjct: 185 AYKLNASKMREAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGS 244

Query: 268 GFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYAS-GVPPQGLCLVSINYK 327
           GFLYRQVRNMV LL+QIGKEAL  ++VP IL ++DRR LAKY S    P GLCLVS+ YK
Sbjct: 245 GFLYRQVRNMVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYK 304

Query: 328 EDHLKLPSDCPGTSFGRTRSVSKCKVPFY 356
           EDHLKLP DCP TS GR  +++KCK+PFY
Sbjct: 305 EDHLKLPLDCPVTSSGRHHTITKCKLPFY 323

BLAST of CsaV3_6G000260 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 79.7 bits (195), Expect = 5.2e-15
Identity = 80/265 (30.19%), Postives = 114/265 (43.02%), Query Frame = 0

Query: 78  LLIAYDGTRYAGWQYQ-RSPPTIQCLIEEALIGATKL--ERKDLHLVGASRTDKGVHAWG 137
           L I Y G R+ G+  + +  P+I+  + +AL     L  ++KD       RTDKGV + G
Sbjct: 94  LKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTG 153

Query: 138 QV------AHFVTPFNYDSLESVHA------------ALNGLLPVDIRVREMSPAVPDFH 197
           QV      +   +P   DS   V+              LN  LP DIRV   SPA  DFH
Sbjct: 154 QVIALFLRSRLKSPPG-DSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFH 213

Query: 198 ARFSTLSKVYHYKIYSGTIMDPFQRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAF---- 257
           ARFS  ++ Y Y  +                   LN +AM  A   F+G+HDF  F    
Sbjct: 214 ARFSCYAREYKYFFWR----------------QNLNLSAMDFAGKKFIGEHDFRNFCKMD 273

Query: 258 -TNAAHNDRVVNPVKVIFRFDVIEMGALVQLEVEGSGFLYRQVRNMVGLLVQIGKEALPP 315
             N     R V   +V    +  E   L    + GS FL+ Q+R MV +L  IG+     
Sbjct: 274 VANVHCYTRRVTFFEVSPCQNSHEGDQLCTFTMRGSAFLWHQIRCMVAVLFMIGQGVESV 333