CsaV3_3G031040 (gene) Cucumber (Chinese Long) v3

Overview


Name	CsaV3_3G031040
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Chinese Long (Cucumber (Chinese Long) v3)
Description	Pectinesterase
Location	chr3: 26823594 .. 26827365 (-)
RNA-Seq Expression	CsaV3_3G031040
Synteny	CsaV3_3G031040

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGTTATATTTTCAATTTTTTAAAAAAATGGTACCATCAACTTGGTAGTTATTATATTTAGCAAAAATATTTTAAATATTTTGAAATGTACTCTACATAAACCCGACCTCAAATGCCTTGTTGACACCGTTACAAACCCAACCCACAAATACTCACGCTAAAATCCCTCCACCACCACACATCTTCAATCATCCCACCGCCACCACCAATGTCAAAACCCGACGACACCCCCGCCGCCGGAGTTCCATTCTCCGGCTACTCAAAAACCAACCTCTTGGTCTTCGCCCTCTCAACAACGCTTCTCCTCGCAGTCGTCTCCTCCGACGACCACGTGGGAAGCAAGTGCGCCATGACACTATACCCCGAGCTTTGCGAGACGACGATATCCACCGCCGTGGGAAGTAGTTCAAAAGAGGCAATCGAAGCGTCGGTGAACATTACGATTAGTGCAGTGAAGGATAATTATAAAAGGGTGCAGAAATTGCTTAAGACAGTGAAGAATCTGACGAAACGACAGAAGATTGCATTTCATGACTGTTTGGAAACGGGGGAAGAGACGCTGAGAGAGCTTTATGAAGTTGTTGAAGATGTTAATGAGTATCCTAAGAAAAAATCTCTCTCACGCTACGCTGATGACCTCAAAACTTTACTCAGTTCCACCATTACTAATCAGGTACGACTCTTCCATTAATTACACCATTGCGAATAAATTATCTCAGAAGATTCACATTTTGAAGTTTTCTGATCTTTCATTTTATGAGCCCAAGACCAAGACATTTATAAACGCATAGAGAAAAAAAACAACTTTTTTTTCCCTTGAGTAACAACAATGTAGAGTTCGTAGATTTGAGTTTTGAAACTCAATTGATTTATTGCTTGCCATATTTGTTAGAAATTCAAAGGTTTCATTTAATATTATCCCTATTTACTTTAATTTCTTACCATCGTTCAAGTACCAGGCCGGTTGATTCTTTGACAAATTCCAAGAAGAAAATAACCTTTTTTTTTCAACCATTTGCTTAGTTTTTCAAAGTATTGGTTGAAAGGAGATATCAAAACAAACATTTTAGATTGGAAAATGAGGGGGTAGGCTTAACTTTTCTAAAATTTAGATAAATAGGATATGGAGTAGAGAGCAGCTGAAAAGAAAAAAAAAAAAAGGCTGGAATGAGAACGATGTTTCATTGAATAATTCAGGAGACATGCGTGGACGGATTCTCACACGACAAAGGCGATAAAAAAGTACGAGAGTCATTAAAAGAAGGCCTTATTCACATTGAAAAGCTTTGCAGCATTGCTTTAGCCTTGATTAAGAACCTTACCGACACGGACATTGCCAATCTCAACAACAACAATAATAATAACCACCTCAACCGAAAGCAACTGGAGGAAAAGAAGATGGAGGATGGAATTAAGTGGCCAGATTGGATGTCACCCAAGGATCGAAGGCTTTTGCAAGCCTCGTCTACAGCCACTCCCGACGTGGTAGTAGCAGCCGACGGGAGCGGAGATTTTCGGACAATTTCTGAGGCAGTTGCGGCTGCGCCTAGTAGAAGTAGCCGGAGGTATATAATAAGAATAAAGGCAGGTGTTTATAGAGAAAATGTGAATGTGGCAAGTAGTAAGAGGAATATAATGTTTTGGGGAGATGGAAGGGTTAACACTATAATCACCGGGAATAGAAATGTGGTCGATGGCAGCACTACCTTCAACTCCGCCACCGTTGGTATGTTGTCTTAATACCTTTTTTTTTTTTTCACTCTAAATATTAACATATACATTTTACTAAAAGATTACAAACATAACAAATTCTCTCCAAATATTAGTGACTAAGTTTCTTTTTCTAAAACAAAAGTAATTTACGTCTCAAATACATCTTATATTATATAATCCAAATTAAAATAATTTACATTTTAAACACAAACTATTATAACAAAACTATAATAATTTAGGATTATAATAACTCACTCATTAATTCAAACATATTAAATTTTATCTTAAATATAAAATAATATTAAGTAATAGAAGATAAATATTGATACTATTTACGAAATGATGAAAGAAAATTTAATACTATAAAGAGAGTTTGAGAAGTTTTATTATATTTTGTAAATATGTTTCATTCCTTTTACTCCATTTAAAAGTGAGAATGGTACGAATTGTTAAGTAATTTTGCTAAATTTAACAATTTTAAAAAAAATTCAACTCCAACCTTACATTATATATAATTTTTTGAGAATTTTGTTTTTGACAAATTATAAAACAAAAAATTATGTTGATTGTTTTTTCTTTACAATTTCTTATATTTTTAAATATATACATATATTATCAATTTGTATCACTCAAAAGTATTTGAAAGATACATTTTTAAAAGATTAAAAAATTGTCATAAGGTAAATATTATGAACATTTTTTTTTTATAGTTTAAGGTGAATACTACAAGACGGGTTAAATTAAAAGTTTTGAACAAAAATTGTAAGCATTTTATTTTTGTTTGACGAAATCTTTAAAGAAAAACGAGAAATTAATTCATAATTAGATATTAATGAGTTTGAATATTATTTCTGTAAATATGTCATATGTAGACATAAATGTATAGCATTAATTTCTTTAAAAAGAAAATTGTTTTTGTGTTTCTAAAATTAGCATTTGAGATTTTGGGAAATGAAAGAAATAATTAAATAAAAATAGAAAATATATTTGAAAGTGAAATAAAGGTGGAATGGATCCGGCAAGTCTTGCACTCTCATTTTCATTACCACCCGTATCTGCCTGCACTATTAAATTCCCCCCGTAGCAAATAATGTTGTGATTTTATTAGGGTAAATAGATATGCTTTTCTCTATTTTTCAAAAAACAATTTACGAAAGTTTAATTAGAGTCAGCTTAGTTGTTTAATGTTTAATGTGGGATTTGAAATTGCAGCGGCGGTAGGAGAAAGGTTCTTGGCGAGAGACGTTACATTTCAGAACACCGCCGGTCCATCAAAGCACCAAGCCGTGGCTCTCCGAGTTGGCTCCGACCTCTCTGCTTTTTACCGCTGCGACATGCTCGCCTATCAAGACACACTCTACGTCCATTCCAACCGTCAATTCTACGTCCAGTGCATCATCGTCGGCACCATCGACTTCATCTTCGGCAATGCCGCCGCCGTCATCCAAGACTGTGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAGGAACATGGTCACTGCACAAGGAAGAACAGACCCAAATCAAAACACTGGGATTGTCATCCAGAAATGCCGAATCGGAACCACCTCCGATTTGCGGCCAGTGATCAGCAATTTCCCGACGTTTCTGGGGCGGCCGTGGCAGAGATACTCGAGAACGGTGGTTATGCAGACGAGTATTAGCAATGTGATTGATCCAGCGGGGTGGCATGTTTGGGATGGGAATTTTGCGCTTGATACTCTGTTTTATGCAGAGTATCAGAATAGTGGTGCGGGTGCCGATACGTCGAGGAGAGTGAAGTGGAAGGGGTTTAGGGTTCTTACCAGGGCGGCGGAAGCGGAGGCCTTCACGGCGGGGAATTTCATCGGCGGCGGGACCTGGCTGAGCTCTACAGGATTCCCATTTTCACTTGGGTTGTAAAATATATATGAGGTACCGACTTGGCGGTGGAATGAGTAATTGTTGTTTTGTTGTGTATTTATTTATGACATTAATCGTTTCTAGTTTTCAAAATATTTGATTGTTTCTTCTTAAAAGTTAGTTTGAGTTAATTGCAAAACCTAAATTTTATTTAT

mRNA sequence

ATGTCAAAACCCGACGACACCCCCGCCGCCGGAGTTCCATTCTCCGGCTACTCAAAAACCAACCTCTTGGTCTTCGCCCTCTCAACAACGCTTCTCCTCGCAGTCGTCTCCTCCGACGACCACGTGGGAAGCAAGTGCGCCATGACACTATACCCCGAGCTTTGCGAGACGACGATATCCACCGCCGTGGGAAGTAGTTCAAAAGAGGCAATCGAAGCGTCGGTGAACATTACGATTAGTGCAGTGAAGGATAATTATAAAAGGGTGCAGAAATTGCTTAAGACAGTGAAGAATCTGACGAAACGACAGAAGATTGCATTTCATGACTGTTTGGAAACGGGGGAAGAGACGCTGAGAGAGCTTTATGAAGTTGTTGAAGATGTTAATGAGTATCCTAAGAAAAAATCTCTCTCACGCTACGCTGATGACCTCAAAACTTTACTCAGTTCCACCATTACTAATCAGGAGACATGCGTGGACGGATTCTCACACGACAAAGGCGATAAAAAAGTACGAGAGTCATTAAAAGAAGGCCTTATTCACATTGAAAAGCTTTGCAGCATTGCTTTAGCCTTGATTAAGAACCTTACCGACACGGACATTGCCAATCTCAACAACAACAATAATAATAACCACCTCAACCGAAAGCAACTGGAGGAAAAGAAGATGGAGGATGGAATTAAGTGGCCAGATTGGATGTCACCCAAGGATCGAAGGCTTTTGCAAGCCTCGTCTACAGCCACTCCCGACGTGGTAGTAGCAGCCGACGGGAGCGGAGATTTTCGGACAATTTCTGAGGCAGTTGCGGCTGCGCCTAGTAGAAGTAGCCGGAGGTATATAATAAGAATAAAGGCAGGTGTTTATAGAGAAAATGTGAATGTGGCAAGTAGTAAGAGGAATATAATGTTTTGGGGAGATGGAAGGGTTAACACTATAATCACCGGGAATAGAAATGTGGTCGATGGCAGCACTACCTTCAACTCCGCCACCGTTGCGGCGGTAGGAGAAAGGTTCTTGGCGAGAGACGTTACATTTCAGAACACCGCCGGTCCATCAAAGCACCAAGCCGTGGCTCTCCGAGTTGGCTCCGACCTCTCTGCTTTTTACCGCTGCGACATGCTCGCCTATCAAGACACACTCTACGTCCATTCCAACCGTCAATTCTACGTCCAGTGCATCATCGTCGGCACCATCGACTTCATCTTCGGCAATGCCGCCGCCGTCATCCAAGACTGTGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAGGAACATGGTCACTGCACAAGGAAGAACAGACCCAAATCAAAACACTGGGATTGTCATCCAGAAATGCCGAATCGGAACCACCTCCGATTTGCGGCCAGTGATCAGCAATTTCCCGACGTTTCTGGGGCGGCCGTGGCAGAGATACTCGAGAACGGTGGTTATGCAGACGAGTATTAGCAATGTGATTGATCCAGCGGGGTGGCATGTTTGGGATGGGAATTTTGCGCTTGATACTCTGTTTTATGCAGAGTATCAGAATAGTGGTGCGGGTGCCGATACGTCGAGGAGAGTGAAGTGGAAGGGGTTTAGGGTTCTTACCAGGGCGGCGGAAGCGGAGGCCTTCACGGCGGGGAATTTCATCGGCGGCGGGACCTGGCTGAGCTCTACAGGATTCCCATTTTCACTTGGGTTGTAA

Coding sequence (CDS)

ATGTCAAAACCCGACGACACCCCCGCCGCCGGAGTTCCATTCTCCGGCTACTCAAAAACCAACCTCTTGGTCTTCGCCCTCTCAACAACGCTTCTCCTCGCAGTCGTCTCCTCCGACGACCACGTGGGAAGCAAGTGCGCCATGACACTATACCCCGAGCTTTGCGAGACGACGATATCCACCGCCGTGGGAAGTAGTTCAAAAGAGGCAATCGAAGCGTCGGTGAACATTACGATTAGTGCAGTGAAGGATAATTATAAAAGGGTGCAGAAATTGCTTAAGACAGTGAAGAATCTGACGAAACGACAGAAGATTGCATTTCATGACTGTTTGGAAACGGGGGAAGAGACGCTGAGAGAGCTTTATGAAGTTGTTGAAGATGTTAATGAGTATCCTAAGAAAAAATCTCTCTCACGCTACGCTGATGACCTCAAAACTTTACTCAGTTCCACCATTACTAATCAGGAGACATGCGTGGACGGATTCTCACACGACAAAGGCGATAAAAAAGTACGAGAGTCATTAAAAGAAGGCCTTATTCACATTGAAAAGCTTTGCAGCATTGCTTTAGCCTTGATTAAGAACCTTACCGACACGGACATTGCCAATCTCAACAACAACAATAATAATAACCACCTCAACCGAAAGCAACTGGAGGAAAAGAAGATGGAGGATGGAATTAAGTGGCCAGATTGGATGTCACCCAAGGATCGAAGGCTTTTGCAAGCCTCGTCTACAGCCACTCCCGACGTGGTAGTAGCAGCCGACGGGAGCGGAGATTTTCGGACAATTTCTGAGGCAGTTGCGGCTGCGCCTAGTAGAAGTAGCCGGAGGTATATAATAAGAATAAAGGCAGGTGTTTATAGAGAAAATGTGAATGTGGCAAGTAGTAAGAGGAATATAATGTTTTGGGGAGATGGAAGGGTTAACACTATAATCACCGGGAATAGAAATGTGGTCGATGGCAGCACTACCTTCAACTCCGCCACCGTTGCGGCGGTAGGAGAAAGGTTCTTGGCGAGAGACGTTACATTTCAGAACACCGCCGGTCCATCAAAGCACCAAGCCGTGGCTCTCCGAGTTGGCTCCGACCTCTCTGCTTTTTACCGCTGCGACATGCTCGCCTATCAAGACACACTCTACGTCCATTCCAACCGTCAATTCTACGTCCAGTGCATCATCGTCGGCACCATCGACTTCATCTTCGGCAATGCCGCCGCCGTCATCCAAGACTGTGACATCCACGCCCGCCGCCCCAATCCCGGCCAGAGGAACATGGTCACTGCACAAGGAAGAACAGACCCAAATCAAAACACTGGGATTGTCATCCAGAAATGCCGAATCGGAACCACCTCCGATTTGCGGCCAGTGATCAGCAATTTCCCGACGTTTCTGGGGCGGCCGTGGCAGAGATACTCGAGAACGGTGGTTATGCAGACGAGTATTAGCAATGTGATTGATCCAGCGGGGTGGCATGTTTGGGATGGGAATTTTGCGCTTGATACTCTGTTTTATGCAGAGTATCAGAATAGTGGTGCGGGTGCCGATACGTCGAGGAGAGTGAAGTGGAAGGGGTTTAGGGTTCTTACCAGGGCGGCGGAAGCGGAGGCCTTCACGGCGGGGAATTTCATCGGCGGCGGGACCTGGCTGAGCTCTACAGGATTCCCATTTTCACTTGGGTTGTAA

Protein sequence

MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTISTAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL*

Homology

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BLAST of CsaV3_3G031040 vs. NCBI nr
Match: XP_004144447.2 (pectinesterase [Cucumis sativus] >KGN58477.1 hypothetical protein Csa_002294 [Cucumis sativus])

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
           MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS
Sbjct: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60

Query: 61  TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
           TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE
Sbjct: 61  TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120

Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180
           LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180

Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
           HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240

Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
           LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300

Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
           IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360

Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
           VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420

Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
           PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480

Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
           SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540

Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
           GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 561

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BLAST of CsaV3_3G031040 vs. NCBI nr
Match: XP_008460358.1 (PREDICTED: pectinesterase-like [Cucumis melo] >KAA0053588.1 pectinesterase-like [Cucumis melo var. makuwa] >TYK19178.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1037.3 bits (2681), Expect = 4.7e-299
Identity = 530/561 (94.47%), Postives = 541/561 (96.43%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
           MSKPD+T AAGVPFS  SKTNLLVFALSTTLLLA VSSDDHV SKCAMTLYPELCETTIS
Sbjct: 1   MSKPDETLAAGVPFSCNSKTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTIS 60

Query: 61  TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
           TAVGSSSKEAIEASVNITI AV DNY+RV+KLLKT  +LTKR+KIA HDCLETGEETLRE
Sbjct: 61  TAVGSSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRE 120

Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180
           LYEVVEDVNEYP KKSLSRYADDLKTLLSS ITNQETC+DGFSHDK DKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLI 180

Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
           HIEKLCSIALALIKNLTDTDIANL  NNNNNHL+RKQL+EK  +D I WPDWMSP DRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANL--NNNNNHLSRKQLKEKNTDDRINWPDWMSPNDRRL 240

Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
           LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSS+RYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRN 300

Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
           IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360

Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
           VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420

Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
           PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480

Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
           SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRV+WKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTA 540

Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
           GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 559

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BLAST of CsaV3_3G031040 vs. NCBI nr
Match: XP_038878011.1 (pectinesterase-like [Benincasa hispida])

HSP 1 Score: 919.1 bits (2374), Expect = 1.9e-263
Identity = 478/575 (83.13%), Postives = 515/575 (89.57%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSGYS------KTNLLVFALSTTLLLA-VVSSDDHVGSKCAMTLYPE 60
           M++ DD+ AAGV FSG        K+ LLV A+S TLL+A V SSD+ V S CA+TLYPE
Sbjct: 1   MAQTDDSAAAGVSFSGRPISISNIKSKLLVLAMSATLLVAGVSSSDEQVRSTCAVTLYPE 60

Query: 61  LCETTISTAVGS--SSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCL 120
           LCE TIST  GS  SSK+AIEASVNITI AVKDNYKR++KL+KTVKNLTKR+KIA +DCL
Sbjct: 61  LCEKTISTGGGSRWSSKDAIEASVNITIGAVKDNYKRMEKLVKTVKNLTKREKIALNDCL 120

Query: 121 ETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKV 180
           E+ EETLRELYEVVED++EYP KKSL++YADDLKTLLSS ITNQETCVDGFSHDK DKKV
Sbjct: 121 ESEEETLRELYEVVEDLHEYPNKKSLTQYADDLKTLLSSAITNQETCVDGFSHDKADKKV 180

Query: 181 RESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEE-----KKMEDG 240
           RES++ GLIHI+KLCSIALALIKNLTDTDIA    N+N N LNRK L E     +  +D 
Sbjct: 181 RESIEAGLIHIKKLCSIALALIKNLTDTDIA----NHNYNVLNRKHLAEVNNGGRGNDDR 240

Query: 241 IKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAG 300
           I WPDWMS  DRRLLQASSTATPDVVVAADG+G+FRT+SEAVAAAPSRSSRRYIIRIKAG
Sbjct: 241 INWPDWMSSGDRRLLQASSTATPDVVVAADGTGNFRTVSEAVAAAPSRSSRRYIIRIKAG 300

Query: 301 VYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQ 360
           VYRENVNV SSK N+MFWGDGR  TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TFQ
Sbjct: 301 VYRENVNVPSSKSNLMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGARFLARDITFQ 360

Query: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAA 420
           NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFY++CIIVGTIDFIFGNAA
Sbjct: 361 NTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYIKCIIVGTIDFIFGNAA 420

Query: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLG 480
           AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+ VI NFPTFLG
Sbjct: 421 AVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQQVIGNFPTFLG 480

Query: 481 RPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGF 540
           RPWQRYSRTVVMQTSISNVIDPAGWHVW+GNFALDTLFYAEYQNSGAGADTSRRV WKG+
Sbjct: 481 RPWQRYSRTVVMQTSISNVIDPAGWHVWNGNFALDTLFYAEYQNSGAGADTSRRVTWKGY 540

Query: 541 RVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
           RV+T AAEA+AFTAGNFI GGTWLSSTGFPFSLGL
Sbjct: 541 RVITSAAEAQAFTAGNFISGGTWLSSTGFPFSLGL 571

[Close]

BLAST of CsaV3_3G031040 vs. NCBI nr
Match: XP_022980581.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 897.1 bits (2317), Expect = 7.6e-257
Identity = 463/576 (80.38%), Postives = 497/576 (86.28%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFS------GYSKTNLLVFALSTTLLLAVVSSDDHVG---SKCAMTLY 60
           M++ DD+  AG P +         K NL + ALS  LLL  VSSDDHV    S CA+TLY
Sbjct: 1   MAQTDDSTVAGAPITSSPTSISKKKHNLFILALSAALLLDGVSSDDHVAAVRSTCAVTLY 60

Query: 61  PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120
           PELC++TISTAV       SSSK+ IEASVNITISAVK NYK ++KL++T KNLTKR+KI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKREKI 120

Query: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHD 180
           A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSS ITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240
           + DKK+RESL  G IH+EKLCS+ALALIKNLTDTDIAN      NN   R     +    
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSE---- 240

Query: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300
              WPDWMSP DR+LLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 -FNWPDWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300

Query: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360
           GVYRENVNV SSK NIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TF
Sbjct: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITF 360

Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420
           QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420

Query: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480
           AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480

Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540
           GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAE+QNSGAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKG 540

Query: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
           +RV++ AAEAE FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 YRVISSAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571

[Close]

BLAST of CsaV3_3G031040 vs. NCBI nr
Match: XP_023526589.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 896.7 bits (2316), Expect = 1.0e-256
Identity = 464/576 (80.56%), Postives = 499/576 (86.63%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSG------YSKTNLLVFALSTTLLLAVVSSDDH---VGSKCAMTLY 60
           M++ DD+  AG P +G        K NL + ALS  +LLA VSSDDH   V S CA+TLY
Sbjct: 1   MAQTDDSTVAGAPSTGSPTSISNKKHNLFILALSAAVLLAGVSSDDHVTAVRSTCAVTLY 60

Query: 61  PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120
           PELC++TISTAV       SSSK+ IEASVNITISAVK NYK ++KLL+T K+LTKR+KI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREKI 120

Query: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHD 180
           A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSS ITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240
           + DKK+RESL  G IH+EKLCS+ALALIKNLTDTDIA        NH N +       E 
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIA--------NHYNIRGRSMAMSE- 240

Query: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300
              WPDWMSP DRRLLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 -FNWPDWMSPGDRRLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300

Query: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360
           GVYRENVNV SSK NIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TF
Sbjct: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITF 360

Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420
           QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420

Query: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480
           AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480

Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540
           GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQNSGAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNSGAGADTSRRVTWKG 540

Query: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
           +RV++ AA+AE+FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 YRVISSAADAESFTAGKFISGGTWLSSTGFPFSLGL 566

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy Swiss-Prot
Match: O04886 (Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1)

HSP 1 Score: 711.1 bits (1834), Expect = 1.0e-203
Identity = 358/523 (68.45%), Postives = 427/523 (81.64%), Query Frame = 0


Query: 44  SKCAMTLYPELCETTISTAVG-----SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKN 103
           S C+ T YP+LC + I+         +S K+ IE S+NIT +AV+ NY  +QKLLK   N
Sbjct: 71  SSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT-N 130

Query: 104 LTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETC 163
           LTKR+K+A HDCLET +ETL EL++ VED+ EYP KKSLS++ADDLKTL+S+ +TNQ TC
Sbjct: 131 LTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTC 190

Query: 164 VDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQL 223
           +DGFSHD  +K VR++L +G +H+EK+CS ALA+IKN+TDTD+  +  +N     NRK  
Sbjct: 191 LDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN-----NRKLT 250

Query: 224 EEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRR 283
           EE    DG  WP W+SP DRRLLQ SS+ TP+ VVAADGSG+F+T++ AVAAAP   ++R
Sbjct: 251 EETSTVDG--WPAWLSPGDRRLLQ-SSSVTPNAVVAADGSGNFKTVAAAVAAAPQGGTKR 310

Query: 284 YIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERF 343
           YIIRIKAGVYRENV V    +NIMF GDGR  TIITG+RNVVDGSTTF SAT A VGE F
Sbjct: 311 YIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATAAVVGEGF 370

Query: 344 LARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTI 403
           LARD+TFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQF+V C+I GT+
Sbjct: 371 LARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTV 430

Query: 404 DFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVI 463
           DFIFGNAAAV+Q+CDIHAR+PN GQ+NMVTAQGRTDPNQNTGIVIQK RIG TSDL+PV 
Sbjct: 431 DFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRTDPNQNTGIVIQKSRIGATSDLKPVQ 490

Query: 464 SNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTS 523
            +FPT+LGRPW+ YSRTV+MQ+SI+++I PAGWH WDGNFAL+TLFY E+QNSGAGA TS
Sbjct: 491 GSFPTYLGRPWKEYSRTVIMQSSITDLIHPAGWHEWDGNFALNTLFYGEHQNSGAGAGTS 550

Query: 524 RRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
            RVKWKGFRV+T A EA+AFT G+FI G +WL STGFPFSLGL
Sbjct: 551 GRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy Swiss-Prot
Match: P83948 (Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1)

HSP 1 Score: 709.1 bits (1829), Expect = 3.9e-203
Identity = 357/523 (68.26%), Postives = 428/523 (81.84%), Query Frame = 0


Query: 44  SKCAMTLYPELCETTISTAVG-----SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKN 103
           S C+ T YP+LC + I+         +S K+ IE S+NIT +AV+ NY  +QKLLK   N
Sbjct: 71  SSCSSTRYPDLCFSAIAAVPEASKKVTSQKDVIEMSLNITTTAVEHNYFGIQKLLKRT-N 130

Query: 104 LTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETC 163
           LTKR+K+A HDCLET +ETL EL++ VED+ EYP KKSLS++ADDLKTL+S+ +TNQ TC
Sbjct: 131 LTKREKVALHDCLETIDETLDELHKAVEDLEEYPNKKSLSQHADDLKTLMSAAMTNQGTC 190

Query: 164 VDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQL 223
           +DGFSHD  +K VR++L +G +H+EK+CS ALA+IKN+TDTD+  +  +N     NRK +
Sbjct: 191 LDGFSHDDANKHVRDALSDGQVHVEKMCSNALAMIKNMTDTDMMIMRTSN-----NRKLI 250

Query: 224 EEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRR 283
           EE    DG  WP W+S  DRRLLQ SS+ TP+VVVAADGSG+F+T++ +VAAAP   ++R
Sbjct: 251 EETSTVDG--WPAWLSTGDRRLLQ-SSSVTPNVVVAADGSGNFKTVAASVAAAPQGGTKR 310

Query: 284 YIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERF 343
           YIIRIKAGVYRENV V    +NIMF GDGR  TIITG+RNVVDGSTTF SATVA VGE F
Sbjct: 311 YIIRIKAGVYRENVEVTKKHKNIMFIGDGRTRTIITGSRNVVDGSTTFKSATVAVVGEGF 370

Query: 344 LARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTI 403
           LARD+TFQNTAGPSKHQAVALRVG+DLSAFY CDMLAYQDTLYVHSNRQF+V C+I GT+
Sbjct: 371 LARDITFQNTAGPSKHQAVALRVGADLSAFYNCDMLAYQDTLYVHSNRQFFVNCLIAGTV 430

Query: 404 DFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVI 463
           DFIFGNAAAV+Q+CDIHAR+PN GQ+NMVTAQGR DPNQNTGIVIQK RIG TSDL+PV 
Sbjct: 431 DFIFGNAAAVLQNCDIHARKPNSGQKNMVTAQGRADPNQNTGIVIQKSRIGATSDLKPVQ 490

Query: 464 SNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTS 523
            +FPT+LGRPW+ YSRTV+MQ+SI++VI PAGWH WDGNFAL+TLFY E+QN+GAGA TS
Sbjct: 491 GSFPTYLGRPWKEYSRTVIMQSSITDVIHPAGWHEWDGNFALNTLFYGEHQNAGAGAGTS 550

Query: 524 RRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
            RVKWKGFRV+T A EA+AFT G+FI G +WL STGFPFSLGL
Sbjct: 551 GRVKWKGFRVITSATEAQAFTPGSFIAGSSWLGSTGFPFSLGL 584

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy Swiss-Prot
Match: Q43143 (Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PMEU1 PE=2 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 6.6e-203
Identity = 369/590 (62.54%), Postives = 441/590 (74.75%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVV-----------SSDDH-------- 60
           M++ +D  +  + F    K   L    S  L+ AV+           +SDDH        
Sbjct: 1   MTRVEDFFSKQIDFCKRKKKIYLAIVASVLLVAAVIGVVAGVKSHSKNSDDHADIMAISS 60

Query: 61  -----VGSKCAMTLYPELCETTISTAVG-----SSSKEAIEASVNITISAVKDNYKRVQK 120
                V S C+ TL+PELC + I          +S K+ IE S+NIT+ AV+ NY  V++
Sbjct: 61  SAHAIVKSACSNTLHPELCYSAIVNVSDFSKKVTSQKDVIELSLNITVKAVRRNYYAVKE 120

Query: 121 LLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSST 180
           L+KT K LT R+K+A HDCLET +ETL EL+  VED+  YP KKSL  + +DLKTL+SS 
Sbjct: 121 LIKTRKGLTPREKVALHDCLETMDETLDELHTAVEDLELYPNKKSLKEHVEDLKTLISSA 180

Query: 181 ITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNN 240
           ITNQETC+DGFSHD+ DKKVR+ L +G  H+EK+CS ALA+I N+TDTDIA      N  
Sbjct: 181 ITNQETCLDGFSHDEADKKVRKVLLKGQKHVEKMCSNALAMICNMTDTDIA------NEM 240

Query: 241 HLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAA 300
            L+      K +ED  +WP+W+S  DRRLLQ SST TPDVVVAADGSGD++T+SEAV  A
Sbjct: 241 KLSAPANNRKLVEDNGEWPEWLSAGDRRLLQ-SSTVTPDVVVAADGSGDYKTVSEAVRKA 300

Query: 301 PSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATV 360
           P +SS+RY+IRIKAGVYRENV+V   K NIMF GDG+ NTIIT +RNV DGSTTF+SATV
Sbjct: 301 PEKSSKRYVIRIKAGVYRENVDVPKKKTNIMFMGDGKSNTIITASRNVQDGSTTFHSATV 360

Query: 361 AAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQ 420
             V  + LARD+TFQNTAG SKHQAVAL VGSDLSAFYRCDMLAYQDTLYVHSNRQF+VQ
Sbjct: 361 VRVAGKVLARDITFQNTAGASKHQAVALCVGSDLSAFYRCDMLAYQDTLYVHSNRQFFVQ 420

Query: 421 CIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTT 480
           C++ GT+DFIFGN AAV QDCDIHARRP  GQ+NMVTAQGRTDPNQNTGIVIQKCRIG T
Sbjct: 421 CLVAGTVDFIFGNGAAVFQDCDIHARRPGSGQKNMVTAQGRTDPNQNTGIVIQKCRIGAT 480

Query: 481 SDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNS 540
           SDLRPV  +FPT+LGRPW+ YSRTV+MQ+SI++VI PAGWH W+GNFALDTLFY EY N+
Sbjct: 481 SDLRPVQKSFPTYLGRPWKEYSRTVIMQSSITDVIQPAGWHEWNGNFALDTLFYGEYANT 540

Query: 541 GAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
           GAGA TS RVKWKG +V+T + EA+A+T G FI GG+WLSSTGFPFSLGL
Sbjct: 541 GAGAPTSGRVKWKGHKVITSSTEAQAYTPGRFIAGGSWLSSTGFPFSLGL 583

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy Swiss-Prot
Match: O49006 (Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME3 PE=1 SV=2)

HSP 1 Score: 703.0 bits (1813), Expect = 2.8e-201
Identity = 360/540 (66.67%), Postives = 423/540 (78.33%), Query Frame = 0


Query: 37  SSDDHVGSKCAMTLYPELCETTISTAVG---SSSKEAIEASVNITISAVKDNYKRVQKLL 96
           SS   + S C+ T YPELC + + TA G   +S K+ IEASVN+TI+AV+ NY  V+KL+
Sbjct: 54  SSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLI 113

Query: 97  KTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTIT 156
           K  K LT R+K A HDCLET +ETL EL+E VED++ YP KK+L  +A DLKTL+SS IT
Sbjct: 114 KKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAIT 173

Query: 157 NQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHL 216
           NQETC+DGFSHD  DK+VR++L +G IH+E +CS ALA+IKN+TDTDIAN          
Sbjct: 174 NQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSN 233

Query: 217 NRKQLEEKKME------------DGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDF 276
           NRK  EE +              D   WP W+S  DRRLLQ S     D  VAADGSG F
Sbjct: 234 NRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTF 293

Query: 277 RTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVD 336
           +T++ AVAAAP  S++RY+I IKAGVYRENV VA  K+NIMF GDGR  TIITG+RNVVD
Sbjct: 294 KTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVD 353

Query: 337 GSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLY 396
           GSTTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLY
Sbjct: 354 GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLY 413

Query: 397 VHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGI 456
           VHSNRQF+V+C+I GT+DFIFGNAA V+QDCDIHARRPN GQ+NMVTAQGRTDPNQNTGI
Sbjct: 414 VHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGI 473

Query: 457 VIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALD 516
           VIQKCRIG TSDL+ V  +FPT+LGRPW+ YS+TV+MQ++IS+VI P GW  W G FAL+
Sbjct: 474 VIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALN 533

Query: 517 TLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
           TL Y EY N+GAGA T+ RVKW+GF+V+T AAEA+ +TAG FIGGG WLSSTGFPFSLGL
Sbjct: 534 TLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy Swiss-Prot
Match: Q42534 (Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2)

HSP 1 Score: 680.6 bits (1755), Expect = 1.5e-194
Identity = 350/538 (65.06%), Postives = 418/538 (77.70%), Query Frame = 0


Query: 33  LAVVSSDDH--VGSKCAMTLYPELCETTISTAVG---SSSKEAIEASVNITISAVKDNYK 92
           +  +SS  H  + S C+ TLYPELC + ++   G   +S KE IEAS+N+T  AVK NY 
Sbjct: 51  ITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYF 110

Query: 93  RVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTL 152
            V+KL+   K LT R+  A HDCLET +ETL EL+  VED+++YPK+KSL ++ADDLKTL
Sbjct: 111 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTL 170

Query: 153 LSSTITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANL--- 212
           +SS ITNQ TC+DGFS+D  D+KVR++L +G +H+E +CS ALA+IKN+T+TDIAN    
Sbjct: 171 ISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELR 230

Query: 213 -NNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRT 272
             ++   N+ NRK  E     D   WP W+S  DRRLLQ  ST   D  VA DGSGDF T
Sbjct: 231 DKSSTFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQ-GSTIKADATVADDGSGDFTT 290

Query: 273 ISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGS 332
           ++ AVAAAP +S++R++I IKAGVYRENV V   K NIMF GDGR  TIITG+RNVVDGS
Sbjct: 291 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 350

Query: 333 TTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVH 392
           TTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVH
Sbjct: 351 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 410

Query: 393 SNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVI 452
           SNRQF+V+C I GT+DFIFGNAAAV+QDCDI+ARRPN GQ+NMVTAQGR+DPNQNTGIVI
Sbjct: 411 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 470

Query: 453 QKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTL 512
           Q CRIG TSDL  V   FPT+LGRPW+ YSRTV+MQ+ IS+VI P GWH W G+FALDTL
Sbjct: 471 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 530

Query: 513 FYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
            Y EY N G GA T+ RVKWKG++V+T   EA+ FTAG FIGGG WL+STGFPFSL L
Sbjct: 531 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy TrEMBL
Match: A0A0A0LCR6 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1)

HSP 1 Score: 1105.5 bits (2858), Expect = 0.0e+00
Identity = 561/561 (100.00%), Postives = 561/561 (100.00%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
           MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS
Sbjct: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60

Query: 61  TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
           TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE
Sbjct: 61  TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120

Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180
           LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180

Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
           HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240

Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
           LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300

Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
           IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360

Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
           VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420

Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
           PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480

Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
           SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540

Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
           GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 561

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy TrEMBL
Match: A0A5A7UEV0 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G001090 PE=3 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.3e-299
Identity = 530/561 (94.47%), Postives = 541/561 (96.43%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
           MSKPD+T AAGVPFS  SKTNLLVFALSTTLLLA VSSDDHV SKCAMTLYPELCETTIS
Sbjct: 1   MSKPDETLAAGVPFSCNSKTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTIS 60

Query: 61  TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
           TAVGSSSKEAIEASVNITI AV DNY+RV+KLLKT  +LTKR+KIA HDCLETGEETLRE
Sbjct: 61  TAVGSSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRE 120

Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180
           LYEVVEDVNEYP KKSLSRYADDLKTLLSS ITNQETC+DGFSHDK DKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLI 180

Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
           HIEKLCSIALALIKNLTDTDIANL  NNNNNHL+RKQL+EK  +D I WPDWMSP DRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANL--NNNNNHLSRKQLKEKNTDDRINWPDWMSPNDRRL 240

Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
           LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSS+RYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRN 300

Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
           IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360

Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
           VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420

Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
           PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480

Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
           SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRV+WKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTA 540

Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
           GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 559

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy TrEMBL
Match: A0A1S3CCA5 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1)

HSP 1 Score: 1037.3 bits (2681), Expect = 2.3e-299
Identity = 530/561 (94.47%), Postives = 541/561 (96.43%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSGYSKTNLLVFALSTTLLLAVVSSDDHVGSKCAMTLYPELCETTIS 60
           MSKPD+T AAGVPFS  SKTNLLVFALSTTLLLA VSSDDHV SKCAMTLYPELCETTIS
Sbjct: 1   MSKPDETLAAGVPFSCNSKTNLLVFALSTTLLLAAVSSDDHVRSKCAMTLYPELCETTIS 60

Query: 61  TAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRE 120
           TAVGSSSKEAIEASVNITI AV DNY+RV+KLLKT  +LTKR+KIA HDCLETGEETLRE
Sbjct: 61  TAVGSSSKEAIEASVNITIGAVGDNYRRVKKLLKTENSLTKREKIALHDCLETGEETLRE 120

Query: 121 LYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLI 180
           LYEVVEDVNEYP KKSLSRYADDLKTLLSS ITNQETC+DGFSHDK DKKVRESLKEGLI
Sbjct: 121 LYEVVEDVNEYPNKKSLSRYADDLKTLLSSAITNQETCLDGFSHDKADKKVRESLKEGLI 180

Query: 181 HIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRL 240
           HIEKLCSIALALIKNLTDTDIANL  NNNNNHL+RKQL+EK  +D I WPDWMSP DRRL
Sbjct: 181 HIEKLCSIALALIKNLTDTDIANL--NNNNNHLSRKQLKEKNTDDRINWPDWMSPNDRRL 240

Query: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRN 300
           LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSS+RYIIRIKAGVYRENVNVASSKRN
Sbjct: 241 LQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSQRYIIRIKAGVYRENVNVASSKRN 300

Query: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360
           IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR
Sbjct: 301 IMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALR 360

Query: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420
           VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN
Sbjct: 361 VGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPN 420

Query: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480
           PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT
Sbjct: 421 PGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQT 480

Query: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTA 540
           SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRV+WKGFRVLTRAAEAEAFTA
Sbjct: 481 SISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVRWKGFRVLTRAAEAEAFTA 540

Query: 541 GNFIGGGTWLSSTGFPFSLGL 562
           GNFIGGGTWLSSTGFPFSLGL
Sbjct: 541 GNFIGGGTWLSSTGFPFSLGL 559

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy TrEMBL
Match: A0A6J1ITZ4 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1)

HSP 1 Score: 897.1 bits (2317), Expect = 3.7e-257
Identity = 463/576 (80.38%), Postives = 497/576 (86.28%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFS------GYSKTNLLVFALSTTLLLAVVSSDDHVG---SKCAMTLY 60
           M++ DD+  AG P +         K NL + ALS  LLL  VSSDDHV    S CA+TLY
Sbjct: 1   MAQTDDSTVAGAPITSSPTSISKKKHNLFILALSAALLLDGVSSDDHVAAVRSTCAVTLY 60

Query: 61  PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120
           PELC++TISTAV       SSSK+ IEASVNITISAVK NYK ++KL++T KNLTKR+KI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLVETTKNLTKREKI 120

Query: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHD 180
           A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSS ITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240
           + DKK+RESL  G IH+EKLCS+ALALIKNLTDTDIAN      NN   R     +    
Sbjct: 181 RADKKIRESLLAGQIHVEKLCSVALALIKNLTDTDIANHYKLTGNNIQGRSMAMSE---- 240

Query: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300
              WPDWMSP DR+LLQASSTATPDVVVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 -FNWPDWMSPGDRKLLQASSTATPDVVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300

Query: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360
           GVYRENVNV SSK NIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVGERFLARD+TF
Sbjct: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGERFLARDITF 360

Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420
           QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420

Query: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480
           AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480

Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540
           GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAE+QNSGAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEFQNSGAGADTSRRVTWKG 540

Query: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
           +RV++ AAEAE FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 YRVISSAAEAEGFTAGKFISGGTWLSSTGFPFSLGL 571

[Close]

BLAST of CsaV3_3G031040 vs. ExPASy TrEMBL
Match: A0A6J1GV42 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1)

HSP 1 Score: 891.3 bits (2302), Expect = 2.0e-255
Identity = 460/576 (79.86%), Postives = 496/576 (86.11%), Query Frame = 0


Query: 1   MSKPDDTPAAGVPFSG------YSKTNLLVFALSTTLLLAVVSSDDH---VGSKCAMTLY 60
           M++ DD+  AG P +G        K NL +  LS  LLLA VSS+DH   V S CA+TLY
Sbjct: 1   MAQTDDSTVAGAPITGCPTSISNKKHNLFIIVLSAALLLAGVSSEDHVTAVRSTCAVTLY 60

Query: 61  PELCETTISTAVG------SSSKEAIEASVNITISAVKDNYKRVQKLLKTVKNLTKRQKI 120
           PELC++TISTAV       SSSK+ IEASVNITISAVK NYK ++KLL+T K+LTKR+KI
Sbjct: 61  PELCQSTISTAVETSSKKVSSSKDIIEASVNITISAVKQNYKSIKKLLETTKHLTKREKI 120

Query: 121 AFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDGFSHD 180
           A HDCLETGEETLRELYEVVED+NEYPKKKSL +YADDLKTLLSS ITNQETCVDGFSHD
Sbjct: 121 ALHDCLETGEETLRELYEVVEDLNEYPKKKSLPQYADDLKTLLSSAITNQETCVDGFSHD 180

Query: 181 KGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEKKMED 240
           + DKKVRESL  G  H+EKLCS+ALALIKNLTDTDIA        NH N +       E 
Sbjct: 181 RADKKVRESLLAGQFHVEKLCSVALALIKNLTDTDIA--------NHYNIRGRSMAMSE- 240

Query: 241 GIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRTISEAVAAAPSRSSRRYIIRIKA 300
              WPDWMSP DR+LLQASSTATPD VVAADGSGDF+T+S AVAAAPSRSSRRYIIRIKA
Sbjct: 241 -FNWPDWMSPGDRKLLQASSTATPDAVVAADGSGDFQTVSAAVAAAPSRSSRRYIIRIKA 300

Query: 301 GVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERFLARDVTF 360
           GVYRENVNV SSK NIMFWGDGR  TIITGNRNVVDGSTTFNSATVAAVG RFLARD+TF
Sbjct: 301 GVYRENVNVPSSKTNIMFWGDGRTTTIITGNRNVVDGSTTFNSATVAAVGGRFLARDITF 360

Query: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTIDFIFGNA 420
           QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYV CIIVGT+DFIFGNA
Sbjct: 361 QNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVSCIIVGTVDFIFGNA 420

Query: 421 AAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVISNFPTFL 480
           AAVIQ+ DIHARRPNPGQ+NMVTAQGRTDPNQNTGIVIQKCRIGTTSDL+PVI NFPTFL
Sbjct: 421 AAVIQNSDIHARRPNPGQKNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLQPVIRNFPTFL 480

Query: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTSRRVKWKG 540
           GRPWQRYSRTVVMQTSISNVIDPAGWH+WDGNFALDTLFYAEYQN+GAGADTSRRV WKG
Sbjct: 481 GRPWQRYSRTVVMQTSISNVIDPAGWHIWDGNFALDTLFYAEYQNNGAGADTSRRVTWKG 540

Query: 541 FRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
           +R+++ AAEAE+FTAG FI GGTWLSSTGFPFSLGL
Sbjct: 541 YRIISSAAEAESFTAGKFISGGTWLSSTGFPFSLGL 566

[Close]

BLAST of CsaV3_3G031040 vs. TAIR 10
Match: AT3G14310.1 (pectin methylesterase 3 )

HSP 1 Score: 703.0 bits (1813), Expect = 2.0e-202
Identity = 360/540 (66.67%), Postives = 423/540 (78.33%), Query Frame = 0


Query: 37  SSDDHVGSKCAMTLYPELCETTISTAVG---SSSKEAIEASVNITISAVKDNYKRVQKLL 96
           SS   + S C+ T YPELC + + TA G   +S K+ IEASVN+TI+AV+ NY  V+KL+
Sbjct: 54  SSHAVLRSSCSSTRYPELCISAVVTAGGVELTSQKDVIEASVNLTITAVEHNYFTVKKLI 113

Query: 97  KTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTIT 156
           K  K LT R+K A HDCLET +ETL EL+E VED++ YP KK+L  +A DLKTL+SS IT
Sbjct: 114 KKRKGLTPREKTALHDCLETIDETLDELHETVEDLHLYPTKKTLREHAGDLKTLISSAIT 173

Query: 157 NQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHL 216
           NQETC+DGFSHD  DK+VR++L +G IH+E +CS ALA+IKN+TDTDIAN          
Sbjct: 174 NQETCLDGFSHDDADKQVRKALLKGQIHVEHMCSNALAMIKNMTDTDIANFEQKAKITSN 233

Query: 217 NRKQLEEKKME------------DGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDF 276
           NRK  EE +              D   WP W+S  DRRLLQ S     D  VAADGSG F
Sbjct: 234 NRKLKEENQETTVAVDIAGAGELDSEGWPTWLSAGDRRLLQGSGVKA-DATVAADGSGTF 293

Query: 277 RTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVD 336
           +T++ AVAAAP  S++RY+I IKAGVYRENV VA  K+NIMF GDGR  TIITG+RNVVD
Sbjct: 294 KTVAAAVAAAPENSNKRYVIHIKAGVYRENVEVAKKKKNIMFMGDGRTRTIITGSRNVVD 353

Query: 337 GSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLY 396
           GSTTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY CDMLAYQDTLY
Sbjct: 354 GSTTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYNCDMLAYQDTLY 413

Query: 397 VHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGI 456
           VHSNRQF+V+C+I GT+DFIFGNAA V+QDCDIHARRPN GQ+NMVTAQGRTDPNQNTGI
Sbjct: 414 VHSNRQFFVKCLIAGTVDFIFGNAAVVLQDCDIHARRPNSGQKNMVTAQGRTDPNQNTGI 473

Query: 457 VIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALD 516
           VIQKCRIG TSDL+ V  +FPT+LGRPW+ YS+TV+MQ++IS+VI P GW  W G FAL+
Sbjct: 474 VIQKCRIGATSDLQSVKGSFPTYLGRPWKEYSQTVIMQSAISDVIRPEGWSEWTGTFALN 533

Query: 517 TLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
           TL Y EY N+GAGA T+ RVKW+GF+V+T AAEA+ +TAG FIGGG WLSSTGFPFSLGL
Sbjct: 534 TLTYREYSNTGAGAGTANRVKWRGFKVITAAAEAQKYTAGQFIGGGGWLSSTGFPFSLGL 592

[Close]

BLAST of CsaV3_3G031040 vs. TAIR 10
Match: AT1G53830.1 (pectin methylesterase 2 )

HSP 1 Score: 680.6 bits (1755), Expect = 1.0e-195
Identity = 350/538 (65.06%), Postives = 418/538 (77.70%), Query Frame = 0


Query: 33  LAVVSSDDH--VGSKCAMTLYPELCETTISTAVG---SSSKEAIEASVNITISAVKDNYK 92
           +  +SS  H  + S C+ TLYPELC + ++   G   +S KE IEAS+N+T  AVK NY 
Sbjct: 51  ITTLSSTSHAILKSVCSSTLYPELCFSAVAATGGKELTSQKEVIEASLNLTTKAVKHNYF 110

Query: 93  RVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTL 152
            V+KL+   K LT R+  A HDCLET +ETL EL+  VED+++YPK+KSL ++ADDLKTL
Sbjct: 111 AVKKLIAKRKGLTPREVTALHDCLETIDETLDELHVAVEDLHQYPKQKSLRKHADDLKTL 170

Query: 153 LSSTITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANL--- 212
           +SS ITNQ TC+DGFS+D  D+KVR++L +G +H+E +CS ALA+IKN+T+TDIAN    
Sbjct: 171 ISSAITNQGTCLDGFSYDDADRKVRKALLKGQVHVEHMCSNALAMIKNMTETDIANFELR 230

Query: 213 -NNNNNNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVAADGSGDFRT 272
             ++   N+ NRK  E     D   WP W+S  DRRLLQ  ST   D  VA DGSGDF T
Sbjct: 231 DKSSTFTNNNNRKLKEVTGDLDSDGWPKWLSVGDRRLLQ-GSTIKADATVADDGSGDFTT 290

Query: 273 ISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGS 332
           ++ AVAAAP +S++R++I IKAGVYRENV V   K NIMF GDGR  TIITG+RNVVDGS
Sbjct: 291 VAAAVAAAPEKSNKRFVIHIKAGVYRENVEVTKKKTNIMFLGDGRGKTIITGSRNVVDGS 350

Query: 333 TTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVH 392
           TTF+SATVAAVGERFLARD+TFQNTAGPSKHQAVALRVGSD SAFY+CDM AYQDTLYVH
Sbjct: 351 TTFHSATVAAVGERFLARDITFQNTAGPSKHQAVALRVGSDFSAFYQCDMFAYQDTLYVH 410

Query: 393 SNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVI 452
           SNRQF+V+C I GT+DFIFGNAAAV+QDCDI+ARRPN GQ+NMVTAQGR+DPNQNTGIVI
Sbjct: 411 SNRQFFVKCHITGTVDFIFGNAAAVLQDCDINARRPNSGQKNMVTAQGRSDPNQNTGIVI 470

Query: 453 QKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTL 512
           Q CRIG TSDL  V   FPT+LGRPW+ YSRTV+MQ+ IS+VI P GWH W G+FALDTL
Sbjct: 471 QNCRIGGTSDLLAVKGTFPTYLGRPWKEYSRTVIMQSDISDVIRPEGWHEWSGSFALDTL 530

Query: 513 FYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
            Y EY N G GA T+ RVKWKG++V+T   EA+ FTAG FIGGG WL+STGFPFSL L
Sbjct: 531 TYREYLNRGGGAGTANRVKWKGYKVITSDTEAQPFTAGQFIGGGGWLASTGFPFSLSL 587

[Close]

BLAST of CsaV3_3G031040 vs. TAIR 10
Match: AT1G11580.1 (methylesterase PCR A )

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-115
Identity = 222/459 (48.37%), Postives = 295/459 (64.27%), Query Frame = 0


Query: 102 RQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCVDG 161
           R K  F DC E  + +   +   +E++       +L  Y+ ++ T LSS +TN  TC++ 
Sbjct: 118 RDKAGFADCEEMMDVSKDRMMSSMEELR--GGNYNLESYS-NVHTWLSSVLTNYMTCLES 177

Query: 162 FSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLTDTDIANLNNNNNNNHLNRKQLEEK 221
            S    + K  + +K  L  +     +ALA+  ++                      ++ 
Sbjct: 178 ISDVSVNSK--QIVKPQLEDLVSRARVALAIFVSVLPA------------------RDDL 237

Query: 222 KMEDGIKWPDWMSPKDRRLLQASS---TATPDVVVAADGSGDFRTISEAVAAAPSRSSRR 281
           KM    ++P W++  DR+LL++S      T +VVVA DG+G F+T++EAVAAAP  S+ R
Sbjct: 238 KMIISNRFPSWLTALDRKLLESSPKTLKVTANVVVAKDGTGKFKTVNEAVAAAPENSNTR 297

Query: 282 YIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVAAVGERF 341
           Y+I +K GVY+E +++   K+N+M  GDG+  TIITG+ NV+DGSTTF SATVAA G+ F
Sbjct: 298 YVIYVKKGVYKETIDIGKKKKNLMLVGDGKDATIITGSLNVIDGSTTFRSATVAANGDGF 357

Query: 342 LARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQCIIVGTI 401
           +A+D+ FQNTAGP+KHQAVALRV +D +   RC + AYQDTLY H+ RQFY    I GT+
Sbjct: 358 MAQDIWFQNTAGPAKHQAVALRVSADQTVINRCRIDAYQDTLYTHTLRQFYRDSYITGTV 417

Query: 402 DFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTSDLRPVI 461
           DFIFGN+A V Q+CDI AR P  GQ+NM+TAQGR D NQNT I IQKC+I  +SDL PV 
Sbjct: 418 DFIFGNSAVVFQNCDIVARNPGAGQKNMLTAQGREDQNQNTAISIQKCKITASSDLAPVK 477

Query: 462 SNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSGAGADTS 521
            +  TFLGRPW+ YSRTV+MQ+ I N IDPAGW  WDG FAL TL+Y EY N+G GADTS
Sbjct: 478 GSVKTFLGRPWKLYSRTVIMQSFIDNHIDPAGWFPWDGEFALSTLYYGEYANTGPGADTS 537

Query: 522 RRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPF 558
           +RV WKGF+V+  + EAE FT    I GG WL  TG  F
Sbjct: 538 KRVNWKGFKVIKDSKEAEQFTVAKLIQGGLWLKPTGVTF 553

[Close]

BLAST of CsaV3_3G031040 vs. TAIR 10
Match: AT5G53370.1 (pectin methylesterase PCR fragment F )

HSP 1 Score: 404.4 bits (1038), Expect = 1.4e-112
Identity = 222/529 (41.97%), Postives = 321/529 (60.68%), Query Frame = 0


Query: 42  VGSKCAMTLYPELCETTISTAVGSSSKEAIEASVNITISAVKDNYKRVQKLLKTV--KNL 101
           +   C+ +LYP LC  T+    GS + +  E  ++I+ +A    + +      T+    +
Sbjct: 75  ISRTCSKSLYPNLCIDTLLDFPGSLTADENEL-IHISFNATLQKFSKALYTSSTITYTQM 134

Query: 102 TKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSLSRYADDLKTLLSSTITNQETCV 161
             R + A+  CLE  ++++  L   +  V      +S S    D+ T LSS +TN +TC 
Sbjct: 135 PPRVRSAYDSCLELLDDSVDALTRALSSVVVVSGDESHS----DVMTWLSSAMTNHDTCT 194

Query: 162 DGFSHDKGD-KKVRESLKEGLIHIEKLCSIALAL----IKNLTDTDIANLNNNNNNNHLN 221
           DGF   +G   +V++ +   +  + ++ S  LA+    +K+L+   + N          N
Sbjct: 195 DGFDEIEGQGGEVKDQVIGAVKDLSEMVSNCLAIFAGKVKDLSGVPVVN----------N 254

Query: 222 RKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTA-TPDVVVAADGSGDFRTISEAVAAAPS 281
           RK L  ++ E+    P+W+  +DR LL   ++A   D+ V+ DGSG F+TI+EA+  AP 
Sbjct: 255 RKLLGTEETEE---LPNWLKREDRELLGTPTSAIQADITVSKDGSGTFKTIAEAIKKAPE 314

Query: 282 RSSRRYIIRIKAGVY-RENVNVASSKRNIMFWGDGRVNTIITGNRNVVDGSTTFNSATVA 341
            SSRR++I +KAG Y  EN+ V   K N+MF GDG+  T+ITG +++ D  TTF++AT A
Sbjct: 315 HSSRRFVIYVKAGRYEEENLKVGRKKTNLMFIGDGKGKTVITGGKSIADDLTTFHTATFA 374

Query: 342 AVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDMLAYQDTLYVHSNRQFYVQC 401
           A G  F+ RD+TF+N AGP+KHQAVALRVG D +  YRC+++ YQD LYVHSNRQF+ +C
Sbjct: 375 ATGAGFIVRDMTFENYAGPAKHQAVALRVGGDHAVVYRCNIIGYQDALYVHSNRQFFREC 434

Query: 402 IIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTDPNQNTGIVIQKCRIGTTS 461
            I GT+DFIFGNAA ++Q C+I+AR+P   Q+  +TAQ R DPNQNTGI I  C++  T 
Sbjct: 435 EIYGTVDFIFGNAAVILQSCNIYARKPMAQQKITITAQNRKDPNQNTGISIHACKLLATP 494

Query: 462 DLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVWDGNFALDTLFYAEYQNSG 521
           DL     ++PT+LGRPW+ YSR V M + + + IDP GW  W+G FALD+L+Y EY N G
Sbjct: 495 DLEASKGSYPTYLGRPWKLYSRVVYMMSDMGDHIDPRGWLEWNGPFALDSLYYGEYMNKG 554

Query: 522 AGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTGFPFSLGL 562
            G+   +RVKW G+ V+T   EA  FT   FI G +WL STG  F  GL
Sbjct: 555 LGSGIGQRVKWPGYHVITSTVEASKFTVAQFISGSSWLPSTGVSFFSGL 585

[Close]

BLAST of CsaV3_3G031040 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 403.3 bits (1035), Expect = 3.2e-112
Identity = 249/547 (45.52%), Postives = 338/547 (61.79%), Query Frame = 0


Query: 22  LLVFALSTTLLLAVVS--SDDHVGSKCAMTLYPELCETTISTAVGSSSKEAIEASVNITI 81
           ++ F +   L+ + VS  +   V + C+ T  P+ CE  ++    +   ++    + I++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 82  SAVKDN--YKRVQKLLKTVKNLTKRQKIAFHDCLETGEETLRELYEVVEDVNEYPKKKSL 141
             V D     +        K    R+K A+ DC++  + T+ ++ E ++     P  K  
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMD-----PNVKCS 127

Query: 142 SRYADDLKTLLSSTITNQETCVDGFSHDKGDKKVRESLKEGLIHIEKLCSIALALIKNLT 201
                D +T LS+ +TN +TC  GF            L+ G      +  I L L+ N  
Sbjct: 128 KL---DAQTWLSTALTNLDTCRAGF------------LELG------VTDIVLPLMSN-- 187

Query: 202 DTDIANLNNNN---NNNHLNRKQLEEKKMEDGIKWPDWMSPKDRRLLQASSTATPDVVVA 261
             +++NL  N    N    N    E    +DG  +P W+ P DR+LLQ SST   + VVA
Sbjct: 188 --NVSNLLCNTLAINKVPFNYTPPE----KDG--FPSWVKPGDRKLLQ-SSTPKDNAVVA 247

Query: 262 ADGSGDFRTISEAVAAAPSRSSRRYIIRIKAGVYRENVNVASSKRNIMFWGDGRVNTIIT 321
            DGSG+F+TI EA+ AA    S R++I +K GVY EN+ +   K+N+M  GDG   TIIT
Sbjct: 248 KDGSGNFKTIKEAIDAA--SGSGRFVIYVKQGVYSENLEI--RKKNVMLRGDGIGKTIIT 307

Query: 322 GNRNVVDGSTTFNSATVAAVGERFLARDVTFQNTAGPSKHQAVALRVGSDLSAFYRCDML 381
           G+++V  G+TTFNSATVAAVG+ F+AR +TF+NTAG S  QAVALR GSDLS FY+C   
Sbjct: 308 GSKSVGGGTTTFNSATVAAVGDGFIARGITFRNTAGASNEQAVALRSGSDLSVFYQCSFE 367

Query: 382 AYQDTLYVHSNRQFYVQCIIVGTIDFIFGNAAAVIQDCDIHARRPNPGQRNMVTAQGRTD 441
           AYQDTLYVHSNRQFY  C + GT+DFIFGNAAAV+Q+C+I ARRP   + N +TAQGR+D
Sbjct: 368 AYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAVLQNCNIFARRPR-SKTNTITAQGRSD 427

Query: 442 PNQNTGIVIQKCRIGTTSDLRPVISNFPTFLGRPWQRYSRTVVMQTSISNVIDPAGWHVW 501
           PNQNTGI+I   R+   SDLRPV+ +  T+LGRPW++YSRTV M+TS+ ++IDP GW  W
Sbjct: 428 PNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRPWRQYSRTVFMKTSLDSLIDPRGWLEW 487

Query: 502 DGNFALDTLFYAEYQNSGAGADTSRRVKWKGFRVLTRAAEAEAFTAGNFIGGGTWLSSTG 561
           DGNFAL TLFYAE+QN+G GA TS RV W GFRVL  A+EA  FT G F+ GG+W+ S+ 
Sbjct: 488 DGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRVLGSASEASKFTVGTFLAGGSWIPSS- 511

The following BLAST results are available for this feature:

BLAST of CsaV3_3G031040 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_004144447.2	0.0e+00	100.00	pectinesterase [Cucumis sativus] >KGN58477.1 hypothetical protein Csa_002294 [Cu...	[more]
XP_008460358.1	4.7e-299	94.47	PREDICTED: pectinesterase-like [Cucumis melo] >KAA0053588.1 pectinesterase-like ...	[more]
XP_038878011.1	1.9e-263	83.13	pectinesterase-like [Benincasa hispida]	[more]
XP_022980581.1	7.6e-257	80.38	pectinesterase-like [Cucurbita maxima]	[more]
XP_023526589.1	1.0e-256	80.56	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsaV3_3G031040 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04886	1.0e-203	68.45	Pectinesterase 1 OS=Citrus sinensis OX=2711 GN=PECS-1.1 PE=2 SV=1	[more]
P83948	3.9e-203	68.26	Pectinesterase 3 OS=Citrus sinensis OX=2711 PE=1 SV=1	[more]
Q43143	6.6e-203	62.54	Pectinesterase/pectinesterase inhibitor U1 OS=Solanum lycopersicum OX=4081 GN=PM...	[more]
O49006	2.8e-201	66.67	Pectinesterase/pectinesterase inhibitor 3 OS=Arabidopsis thaliana OX=3702 GN=PME...	[more]
Q42534	1.5e-194	65.06	Pectinesterase 2 OS=Arabidopsis thaliana OX=3702 GN=PME2 PE=2 SV=2	[more]

BLAST of CsaV3_3G031040 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LCR6	0.0e+00	100.00	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_3G646640 PE=3 SV=1	[more]
A0A5A7UEV0	2.3e-299	94.47	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold522G00109...	[more]
A0A1S3CCA5	2.3e-299	94.47	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103499204 PE=3 SV=1	[more]
A0A6J1ITZ4	3.7e-257	80.38	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111479907 PE=3 SV=1	[more]
A0A6J1GV42	2.0e-255	79.86	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111457458 PE=3 SV=1	[more]

BLAST of CsaV3_3G031040 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G14310.1	2.0e-202	66.67	pectin methylesterase 3	[more]
AT1G53830.1	1.0e-195	65.06	pectin methylesterase 2	[more]
AT1G11580.1	1.8e-115	48.37	methylesterase PCR A	[more]
AT5G53370.1	1.4e-112	41.97	pectin methylesterase PCR fragment F	[more]
AT2G45220.1	3.2e-112	45.52	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Chinese Long) v3
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 201..221
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 40..560
None	No IPR available	PANTHER	PTHR31707:SF180	PECTINESTERASE	coord: 40..560
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 46..197 e-value: 6.46664E-38 score: 135.258
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 37..192 e-value: 2.6E-33 score: 126.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 44..192 e-value: 4.5E-24 score: 85.5
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 21..197 e-value: 2.0E-19 score: 67.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 36..198 e-value: 1.5E-27 score: 98.4
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 40..197
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 244..561 e-value: 5.9E-125 score: 418.9
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 250..546 e-value: 1.0E-144 score: 480.8
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 275..294
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 394..403
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 244..560

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsaV3_3G031040.1	CsaV3_3G031040.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
molecular_function	GO:0004857	enzyme inhibitor activity
molecular_function	GO:0030599	pectinesterase activity