CsGy6G008150 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy6G008150
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Descriptionmajor pollen allergen Ole e 6-like
LocationGy14Chr6: 6820600 .. 6821086 (-)
RNA-Seq ExpressionCsGy6G008150
SyntenyCsGy6G008150
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCAAGAAAATGATGGCAGTGTTTTTGATGTGCATTGTGGTGGTAACCAGTTTGCAATTTGCCACTGCCAATAAGGAGTATGAAGTGGCCAGTTACGAAGCCAAATTTGATGCCAAGTACAAGGCCTGCTTTGTAACTTGTGAGAAAGAATGCATTGAACGTGGAAGTGGCCACAGTTATTGTGAAGTTAAATGCGACGAAGATTGTGGTGCAAAAGAAGCTGCCGGTACTCATCTCATCCTCTCTTATCTTTCTTTTACTTCTAACATGTTAAATTTAAATTATGATGTAAGTTTAGAATGTACTAACATTATATTTCCATTCTATCGTTTTAAGAAAATTAATCGACGATATATTAACAAAATTTACAATATCTTTTATATACATTTTTTCTTTATTCTTGTAGATTTGTAAATAAAATTTATTCACTATATTTAATTTTAATTGTTGTATTTGTTTTGCAGATAAGTTGCATATCCATTAG

mRNA sequence

ATGGCCAAGAAAATGATGGCAGTGTTTTTGATGTGCATTGTGGTGGTAACCAGTTTGCAATTTGCCACTGCCAATAAGGAGTATGAAGTGGCCAGTTACGAAGCCAAATTTGATGCCAAGTACAAGGCCTGCTTTGTAACTTGTGAGAAAGAATGCATTGAACGTGGAAGTGGCCACAGTTATTGTGAAGTTAAATGCGACGAAGATTGTGGTGCAAAAGAAGCTGCCGATAAGTTGCATATCCATTAG

Coding sequence (CDS)

ATGGCCAAGAAAATGATGGCAGTGTTTTTGATGTGCATTGTGGTGGTAACCAGTTTGCAATTTGCCACTGCCAATAAGGAGTATGAAGTGGCCAGTTACGAAGCCAAATTTGATGCCAAGTACAAGGCCTGCTTTGTAACTTGTGAGAAAGAATGCATTGAACGTGGAAGTGGCCACAGTTATTGTGAAGTTAAATGCGACGAAGATTGTGGTGCAAAAGAAGCTGCCGATAAGTTGCATATCCATTAG

Protein sequence

MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHSYCEVKCDEDCGAKEAADKLHIH*
Homology
BLAST of CsGy6G008150 vs. ExPASy Swiss-Prot
Match: O24172 (Major pollen allergen Ole e 6 OS=Olea europaea OX=4146 GN=OLE6 PE=1 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 9.0e-08
Identity = 20/42 (47.62%), Postives = 32/42 (76.19%), Query Frame = 0

Query: 38 DAKYKACFVTCEKECIERGSGHSYCEVKCDEDCGAKEAADKL 80
          +A++K C+ TC KEC ++G+G ++CE+KCD DC  K+  +KL
Sbjct: 2  EAQFKECYDTCHKECSDKGNGFTFCEMKCDTDCSVKDVKEKL 43

BLAST of CsGy6G008150 vs. NCBI nr
Match: XP_004140522.1 (major pollen allergen Ole e 6 [Cucumis sativus] >KGN46487.1 hypothetical protein Csa_005056 [Cucumis sativus])

HSP 1 Score: 170 bits (430), Expect = 3.25e-53
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS
Sbjct: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60

Query: 61 YCEVKCDEDCGAKEAADKLHIH 82
          YCEVKCDEDCGAKEAADKLHIH
Sbjct: 61 YCEVKCDEDCGAKEAADKLHIH 82

BLAST of CsGy6G008150 vs. NCBI nr
Match: XP_008459802.1 (PREDICTED: major pollen allergen Ole e 6-like [Cucumis melo] >KAA0039792.1 major pollen allergen Ole e 6-like [Cucumis melo var. makuwa] >TYK24705.1 major pollen allergen Ole e 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 150 bits (380), Expect = 1.47e-45
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKMMAVFLMCIVVV +LQFATANKE EVA+YEAKFDAKYKACFV CEKEC+ERG GHS
Sbjct: 1  MAKKMMAVFLMCIVVVATLQFATANKEDEVANYEAKFDAKYKACFVICEKECVERGGGHS 60

Query: 61 YCEVKCDEDCGAKEAADKLHI 81
          YCEVKCDEDCGAKEAADKL I
Sbjct: 61 YCEVKCDEDCGAKEAADKLGI 81

BLAST of CsGy6G008150 vs. NCBI nr
Match: XP_008456958.1 (PREDICTED: major pollen allergen Ole e 6-like [Cucumis melo] >KAA0066995.1 major pollen allergen Ole e 6-like [Cucumis melo var. makuwa] >TYJ96804.1 major pollen allergen Ole e 6-like [Cucumis melo var. makuwa])

HSP 1 Score: 139 bits (350), Expect = 5.57e-41
Identity = 66/81 (81.48%), Postives = 72/81 (88.89%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKM+A+FLMCIVVV +LQFA+ANKE EVA YEAKFDAKYK+CF TCEKEC+E GSG S
Sbjct: 1  MAKKMIALFLMCIVVVAALQFASANKEDEVAKYEAKFDAKYKSCFKTCEKECLENGSGQS 60

Query: 61 YCEVKCDEDCGAKEAADKLHI 81
          YCEVKCDEDC  KE ADKLHI
Sbjct: 61 YCEVKCDEDCNEKEVADKLHI 81

BLAST of CsGy6G008150 vs. NCBI nr
Match: XP_004146471.1 (major pollen allergen Ole e 6 [Cucumis sativus] >KGN50739.1 hypothetical protein Csa_005859 [Cucumis sativus])

HSP 1 Score: 130 bits (328), Expect = 1.23e-37
Identity = 64/81 (79.01%), Postives = 70/81 (86.42%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MA KM+A+FLMCIVVV +LQFA ANKE +VA YEAKFDAKYK+CF TCEKEC+  GSG S
Sbjct: 1  MANKMIALFLMCIVVVAALQFANANKE-DVAKYEAKFDAKYKSCFETCEKECLRYGSGQS 60

Query: 61 YCEVKCDEDCGAKEAADKLHI 81
          YCEVKCDEDC  KEAADKLHI
Sbjct: 61 YCEVKCDEDCDEKEAADKLHI 80

BLAST of CsGy6G008150 vs. NCBI nr
Match: XP_038891517.1 (major pollen allergen Ole e 6-like [Benincasa hispida])

HSP 1 Score: 130 bits (327), Expect = 1.74e-37
Identity = 63/81 (77.78%), Postives = 70/81 (86.42%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKMMAVFLMCIVVV +LQFATAN++ EV  YEAKFDAKYK+C+ TCEKEC+  GSG S
Sbjct: 1  MAKKMMAVFLMCIVVVAALQFATANED-EVTKYEAKFDAKYKSCYETCEKECLANGSGQS 60

Query: 61 YCEVKCDEDCGAKEAADKLHI 81
          +CEVKCDEDC  KE ADKLHI
Sbjct: 61 FCEVKCDEDCDEKEVADKLHI 80

BLAST of CsGy6G008150 vs. ExPASy TrEMBL
Match: A0A0A0KDP7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G102490 PE=4 SV=1)

HSP 1 Score: 170 bits (430), Expect = 1.57e-53
Identity = 82/82 (100.00%), Postives = 82/82 (100.00%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS
Sbjct: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60

Query: 61 YCEVKCDEDCGAKEAADKLHIH 82
          YCEVKCDEDCGAKEAADKLHIH
Sbjct: 61 YCEVKCDEDCGAKEAADKLHIH 82

BLAST of CsGy6G008150 vs. ExPASy TrEMBL
Match: A0A5A7T8P0 (Major pollen allergen Ole e 6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold266G002620 PE=4 SV=1)

HSP 1 Score: 150 bits (380), Expect = 7.14e-46
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKMMAVFLMCIVVV +LQFATANKE EVA+YEAKFDAKYKACFV CEKEC+ERG GHS
Sbjct: 1  MAKKMMAVFLMCIVVVATLQFATANKEDEVANYEAKFDAKYKACFVICEKECVERGGGHS 60

Query: 61 YCEVKCDEDCGAKEAADKLHI 81
          YCEVKCDEDCGAKEAADKL I
Sbjct: 61 YCEVKCDEDCGAKEAADKLGI 81

BLAST of CsGy6G008150 vs. ExPASy TrEMBL
Match: A0A1S3CAJ3 (major pollen allergen Ole e 6-like OS=Cucumis melo OX=3656 GN=LOC103498825 PE=4 SV=1)

HSP 1 Score: 150 bits (380), Expect = 7.14e-46
Identity = 73/81 (90.12%), Postives = 76/81 (93.83%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKMMAVFLMCIVVV +LQFATANKE EVA+YEAKFDAKYKACFV CEKEC+ERG GHS
Sbjct: 1  MAKKMMAVFLMCIVVVATLQFATANKEDEVANYEAKFDAKYKACFVICEKECVERGGGHS 60

Query: 61 YCEVKCDEDCGAKEAADKLHI 81
          YCEVKCDEDCGAKEAADKL I
Sbjct: 61 YCEVKCDEDCGAKEAADKLGI 81

BLAST of CsGy6G008150 vs. ExPASy TrEMBL
Match: A0A5D3BCS2 (Major pollen allergen Ole e 6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold233G00230 PE=4 SV=1)

HSP 1 Score: 139 bits (350), Expect = 2.70e-41
Identity = 66/81 (81.48%), Postives = 72/81 (88.89%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKM+A+FLMCIVVV +LQFA+ANKE EVA YEAKFDAKYK+CF TCEKEC+E GSG S
Sbjct: 1  MAKKMIALFLMCIVVVAALQFASANKEDEVAKYEAKFDAKYKSCFKTCEKECLENGSGQS 60

Query: 61 YCEVKCDEDCGAKEAADKLHI 81
          YCEVKCDEDC  KE ADKLHI
Sbjct: 61 YCEVKCDEDCNEKEVADKLHI 81

BLAST of CsGy6G008150 vs. ExPASy TrEMBL
Match: A0A1S3C530 (major pollen allergen Ole e 6-like OS=Cucumis melo OX=3656 GN=LOC103496750 PE=4 SV=1)

HSP 1 Score: 139 bits (350), Expect = 2.70e-41
Identity = 66/81 (81.48%), Postives = 72/81 (88.89%), Query Frame = 0

Query: 1  MAKKMMAVFLMCIVVVTSLQFATANKEYEVASYEAKFDAKYKACFVTCEKECIERGSGHS 60
          MAKKM+A+FLMCIVVV +LQFA+ANKE EVA YEAKFDAKYK+CF TCEKEC+E GSG S
Sbjct: 1  MAKKMIALFLMCIVVVAALQFASANKEDEVAKYEAKFDAKYKSCFKTCEKECLENGSGQS 60

Query: 61 YCEVKCDEDCGAKEAADKLHI 81
          YCEVKCDEDC  KE ADKLHI
Sbjct: 61 YCEVKCDEDCNEKEVADKLHI 81

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O241729.0e-0847.62Major pollen allergen Ole e 6 OS=Olea europaea OX=4146 GN=OLE6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_004140522.13.25e-53100.00major pollen allergen Ole e 6 [Cucumis sativus] >KGN46487.1 hypothetical protein... [more]
XP_008459802.11.47e-4590.12PREDICTED: major pollen allergen Ole e 6-like [Cucumis melo] >KAA0039792.1 major... [more]
XP_008456958.15.57e-4181.48PREDICTED: major pollen allergen Ole e 6-like [Cucumis melo] >KAA0066995.1 major... [more]
XP_004146471.11.23e-3779.01major pollen allergen Ole e 6 [Cucumis sativus] >KGN50739.1 hypothetical protein... [more]
XP_038891517.11.74e-3777.78major pollen allergen Ole e 6-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
A0A0A0KDP71.57e-53100.00Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G102490 PE=4 SV=1[more]
A0A5A7T8P07.14e-4690.12Major pollen allergen Ole e 6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3CAJ37.14e-4690.12major pollen allergen Ole e 6-like OS=Cucumis melo OX=3656 GN=LOC103498825 PE=4 ... [more]
A0A5D3BCS22.70e-4181.48Major pollen allergen Ole e 6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E56... [more]
A0A1S3C5302.70e-4181.48major pollen allergen Ole e 6-like OS=Cucumis melo OX=3656 GN=LOC103496750 PE=4 ... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015333Pollen allergen ole e 6PFAMPF09253Ole_e_6coord: 44..80
e-value: 1.1E-12
score: 47.7
IPR015333Pollen allergen ole e 6PANTHERPTHR35632MAJOR POLLEN ALLERGEN OLE E 6-LIKEcoord: 1..82
IPR036466Pollen allergen ole e 6 superfamilyGENE3D1.10.287.720Pollen allergen ole e 6coord: 38..82
e-value: 3.6E-16
score: 60.4
IPR036466Pollen allergen ole e 6 superfamilySUPERFAMILY111388Pollen allergen ole e 6coord: 38..79
NoneNo IPR availablePANTHERPTHR35632:SF5SUBFAMILY NOT NAMEDcoord: 1..82

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy6G008150.2CsGy6G008150.2mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0051213 dioxygenase activity