CsGy3G002700 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G002700
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: (RGF1), (RGF2), (RGF3), (RGF4), (RGF5), (RGF6), (RGF7), (RGF8) and (RGF9).
Location	Gy14Chr3: 2137524 .. 2138520 (+)
RNA-Seq Expression	CsGy3G002700
Synteny	CsGy3G002700

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTATGCCTTATTTCTTTCTCATTCTCTTATTCTTCTCATCTACTTTGCATGCATGTGACGCTAGTCGTCTTCGATTCTTCCAGAGTTCGAATTTTGTTTCCAGGATTAATGAACTGCATTCGATGTCTCAAGGGATTTGGAGTATAAATCATGATCAGGAGGGAAGTTTCGACAAAAACAACAATGACAACATTCTTCATAATAAGGAGAATATTATTGGAATTGAAATGTTAAAGGTATACTATACATATATACTTCATCTCCCATCTTGTGCTTTCTAAAGATGAGCTTGTGATTTACTTCATGATTACTAAGCTAGCTATATGAGAGGGGGAGTTTTTAGATATGTTGGACATTTTCATTTAAACATATCTACATCTTGATAAAACTCAATATATCAAAATATATATGAGGCGATAAAAATATATTTTTCCTCTAAATAATGCAATAAATATGTCAACCAAACTATACATGCATACCTTATCTTATTTAAAAATTCAATCAAATGGTTTTTGGTCGAAATATAATGTGTAATTATCGATATCAAATATATTTGATTTTATAAGAAAACCAACCTAATATCCTTTTATCATTATTTCTATTTATATTTGTACAAGCTTTAAAATCTCATAATTTTCATCGACATCAACATTTAATTCTCGAGTCAAGATAATAATAATCATGAATAAAGACACCATTTAAGTTCTTTGGTACAGGACAATAATAATTTCATTAATACAGACAAAGGCATATGAATGATAAATACATGAAAAGGGGAATTATTTGGAAATATAGTGAGTGAGTGATTAATGAATTAACTTTGGTATTTTAATTTATCAGGGAATGAAGAGAGATGGAAGCAGATTGGTAATGGAGTTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGGAAGCCTCCTATTCACAATGAAAAACCCTAG

mRNA sequence

ATGGCTATGCCTTATTTCTTTCTCATTCTCTTATTCTTCTCATCTACTTTGCATGCATGTGACGCTAGTCGTCTTCGATTCTTCCAGAGTTCGAATTTTGTTTCCAGGATTAATGAACTGCATTCGATGTCTCAAGGGATTTGGAGTATAAATCATGATCAGGAGGGAAGTTTCGACAAAAACAACAATGACAACATTCTTCATAATAAGGAGAATATTATTGGAATTGAAATGTTAAAGGGAATGAAGAGAGATGGAAGCAGATTGGTAATGGAGTTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGGAAGCCTCCTATTCACAATGAAAAACCCTAG

Coding sequence (CDS)

ATGGCTATGCCTTATTTCTTTCTCATTCTCTTATTCTTCTCATCTACTTTGCATGCATGTGACGCTAGTCGTCTTCGATTCTTCCAGAGTTCGAATTTTGTTTCCAGGATTAATGAACTGCATTCGATGTCTCAAGGGATTTGGAGTATAAATCATGATCAGGAGGGAAGTTTCGACAAAAACAACAATGACAACATTCTTCATAATAAGGAGAATATTATTGGAATTGAAATGTTAAAGGGAATGAAGAGAGATGGAAGCAGATTGGTAATGGAGTTTCCAATGAAAGAAAAAGTGATGAATTCTAAGGAAAATGAAGTTGGAGAAGACATGGTAGTGATGGATTATGCACAACCCCACAGGAAGCCTCCTATTCACAATGAAAAACCCTAG

Protein sequence

MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDKNNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPHRKPPIHNEKP*

Homology

[Close]

BLAST of CsGy3G002700 vs. ExPASy Swiss-Prot
Match: Q93VK8 (Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 3.9e-05
Identity = 20/24 (83.33%), Postives = 22/24 (91.67%), Query Frame = 0


Query: 106 EVGEDMVVMDYAQPHRKPPIHNEK 130
           E GE++VVMDY QPHRKPPIHNEK
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

[Close]

BLAST of CsGy3G002700 vs. NCBI nr
Match: XP_011650407.1 (uncharacterized protein LOC105434783 [Cucumis sativus] >KGN55873.1 hypothetical protein Csa_010787 [Cucumis sativus])

HSP 1 Score: 259 bits (661), Expect = 6.27e-87
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60
           MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK
Sbjct: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60

Query: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120
           NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH
Sbjct: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNEKP 130
           RKPPIHNEKP
Sbjct: 121 RKPPIHNEKP 130

[Close]

BLAST of CsGy3G002700 vs. NCBI nr
Match: XP_038905997.1 (uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida])

HSP 1 Score: 179 bits (455), Expect = 1.31e-55
Identity = 99/129 (76.74%), Postives = 113/129 (87.60%), Query Frame = 0


Query: 3   MPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQ-EGSFDKN 62
           MPY FLILL FSSTLHAC+A  LRFF+SS+FVSRI E HS+SQGIW+   DQ E SF+++
Sbjct: 1   MPYLFLILLIFSSTLHACEARCLRFFESSSFVSRI-EPHSISQGIWA---DQPERSFEES 60

Query: 63  NNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPHR 122
           N+D + + KE I+GIEMLKGMK+ GSRLVMEFPMKEKV+NSKENEVGEDMVVMDYAQPHR
Sbjct: 61  NSDKLSY-KEIIVGIEMLKGMKKHGSRLVMEFPMKEKVLNSKENEVGEDMVVMDYAQPHR 120

Query: 123 KPPIHNEKP 130
           KPPIHNEKP
Sbjct: 121 KPPIHNEKP 124

[Close]

BLAST of CsGy3G002700 vs. NCBI nr
Match: XP_038905996.1 (uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida])

HSP 1 Score: 175 bits (443), Expect = 9.11e-54
Identity = 99/130 (76.15%), Postives = 113/130 (86.92%), Query Frame = 0


Query: 3   MPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQ-EGSFDKN 62
           MPY FLILL FSSTLHAC+A  LRFF+SS+FVSRI E HS+SQGIW+   DQ E SF+++
Sbjct: 1   MPYLFLILLIFSSTLHACEARCLRFFESSSFVSRI-EPHSISQGIWA---DQPERSFEES 60

Query: 63  NNDNILHNKENIIGIEMLK-GMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 122
           N+D + + KE I+GIEMLK GMK+ GSRLVMEFPMKEKV+NSKENEVGEDMVVMDYAQPH
Sbjct: 61  NSDKLSY-KEIIVGIEMLKQGMKKHGSRLVMEFPMKEKVLNSKENEVGEDMVVMDYAQPH 120

Query: 123 RKPPIHNEKP 130
           RKPPIHNEKP
Sbjct: 121 RKPPIHNEKP 125

[Close]

BLAST of CsGy3G002700 vs. NCBI nr
Match: XP_022154042.1 (uncharacterized protein LOC111021396 [Momordica charantia])

HSP 1 Score: 104 bits (260), Expect = 6.05e-26
Identity = 74/135 (54.81%), Postives = 89/135 (65.93%), Query Frame = 0


Query: 1   MAMPYFFLILLFFSSTLHACDAS-RLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFD 60
           MA P    ILL   STLHACDA  RL+ F+ SN VSR+  L  + Q       DQ    +
Sbjct: 1   MATPSLLFILLL--STLHACDACHRLQLFEYSNPVSRLGLLAPIPQ---EFRTDQP---E 60

Query: 61  KNNNDNILHNKENI--IGIEMLKGMKRDGSRLVMEFPMKEK--VMNSKENEVGEDMVVMD 120
           ++ N NI+H   +   IGI +LKGMK+   RL MEF MKEK  +M+ +ENEVGEDMVVMD
Sbjct: 61  RSLNKNIIHKVSSSVEIGIGILKGMKKH-KRLAMEFSMKEKEKLMSYEENEVGEDMVVMD 120

Query: 121 YAQPHRKPPIHNEKP 130
           YAQPHRKPPIHN+KP
Sbjct: 121 YAQPHRKPPIHNKKP 126

[Close]

BLAST of CsGy3G002700 vs. NCBI nr
Match: XP_023540680.1 (uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 99.8 bits (247), Expect = 6.10e-24
Identity = 65/133 (48.87%), Postives = 85/133 (63.91%), Query Frame = 0


Query: 6   FFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDKNNNDN 65
           FFL LLF S+ LHACDA  L+   SS   S +     +S G  +   ++     K+N+ +
Sbjct: 7   FFLALLFLSTFLHACDARLLKLCHSSRLESCL-----ISPGFRTNQLERSLDESKSNDVS 66

Query: 66  ILHNKENIIGIEMLKGMKRDGSRLVMEFPMKE--------KVMNSKENEVGEDMVVMDYA 125
            + N    +GIE+LKGMK+   RL ME P+++        KV+NS+ENEVGEDMVVMDYA
Sbjct: 67  SMEN----VGIEILKGMKKL-RRLAMELPVRDRDRDKDIDKVVNSEENEVGEDMVVMDYA 126

Query: 126 QPHRKPPIHNEKP 130
           QPHRKPPIHN+KP
Sbjct: 127 QPHRKPPIHNQKP 129

[Close]

BLAST of CsGy3G002700 vs. ExPASy TrEMBL
Match: A0A0A0L298 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1)

HSP 1 Score: 259 bits (661), Expect = 3.04e-87
Identity = 130/130 (100.00%), Postives = 130/130 (100.00%), Query Frame = 0


Query: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60
           MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK
Sbjct: 1   MAMPYFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDK 60

Query: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120
           NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH
Sbjct: 61  NNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMKEKVMNSKENEVGEDMVVMDYAQPH 120

Query: 121 RKPPIHNEKP 130
           RKPPIHNEKP
Sbjct: 121 RKPPIHNEKP 130

[Close]

BLAST of CsGy3G002700 vs. ExPASy TrEMBL
Match: A0A6J1DKL1 (uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021396 PE=4 SV=1)

HSP 1 Score: 104 bits (260), Expect = 2.93e-26
Identity = 74/135 (54.81%), Postives = 89/135 (65.93%), Query Frame = 0


Query: 1   MAMPYFFLILLFFSSTLHACDAS-RLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFD 60
           MA P    ILL   STLHACDA  RL+ F+ SN VSR+  L  + Q       DQ    +
Sbjct: 1   MATPSLLFILLL--STLHACDACHRLQLFEYSNPVSRLGLLAPIPQ---EFRTDQP---E 60

Query: 61  KNNNDNILHNKENI--IGIEMLKGMKRDGSRLVMEFPMKEK--VMNSKENEVGEDMVVMD 120
           ++ N NI+H   +   IGI +LKGMK+   RL MEF MKEK  +M+ +ENEVGEDMVVMD
Sbjct: 61  RSLNKNIIHKVSSSVEIGIGILKGMKKH-KRLAMEFSMKEKEKLMSYEENEVGEDMVVMD 120

Query: 121 YAQPHRKPPIHNEKP 130
           YAQPHRKPPIHN+KP
Sbjct: 121 YAQPHRKPPIHNKKP 126

[Close]

BLAST of CsGy3G002700 vs. ExPASy TrEMBL
Match: A0A6J1KZM9 (uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521 PE=4 SV=1)

HSP 1 Score: 95.1 bits (235), Expect = 2.03e-22
Identity = 68/135 (50.37%), Postives = 84/135 (62.22%), Query Frame = 0


Query: 7   FLILLFFSSTLHACDASRLRFFQSSNFVS-RINELHSMSQGIWSINHDQEGSFDKNNNDN 66
           FL LLF S+ LHACDA  L+   SS   S  I+    M Q         + S DK+ + N
Sbjct: 8   FLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQ--------LKRSLDKSKS-N 67

Query: 67  ILHNKENIIGIEMLKGMKRDGSRLVMEFPMKE----------KVMNSKENEVGEDMVVMD 126
            + + EN+ GIE+LKGMK+   RL ME P+++          KV+NS+ENEVGEDMVVMD
Sbjct: 68  EVSSVENV-GIEILKGMKKL-RRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMD 127

Query: 127 YAQPHRKPPIHNEKP 130
           YAQPHRKPPIHN KP
Sbjct: 128 YAQPHRKPPIHNRKP 131

[Close]

BLAST of CsGy3G002700 vs. ExPASy TrEMBL
Match: M1A0Y5 (Uncharacterized protein OS=Solanum tuberosum OX=4113 PE=4 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 4.14e-09
Identity = 30/50 (60.00%), Postives = 38/50 (76.00%), Query Frame = 0


Query: 84  RDGSRLVMEFPM----KEKVMNSKENEVGEDMVVMDYAQPHRKPPIHNEK 129
           R  SRL++E P+    +E+ +NS E E+ ED+VVMDYAQPHRKPPIHN K
Sbjct: 5   RRESRLMLESPLSTQHEEEAVNSIEKELVEDVVVMDYAQPHRKPPIHNTK 54

[Close]

BLAST of CsGy3G002700 vs. ExPASy TrEMBL
Match: A0A6S7NRQ9 (Uncharacterized protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS30448 PE=4 SV=1)

HSP 1 Score: 60.8 bits (146), Expect = 5.93e-09
Identity = 50/138 (36.23%), Postives = 70/138 (50.72%), Query Frame = 0


Query: 16  TLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQEGSFDKNNNDNILHNKENIIG 75
           ++HACD+  L  F          ELH ++     I    EG   +N  +  + +KE+ + 
Sbjct: 15  SMHACDSRHLGVFHKEPG----KELHLITSKAPVIRVGHEG---RNCKETNMEDKESKLE 74

Query: 76  -----IEMLKG------MKRDGS---------RLVMEFPMK---EKVMNSKENEVGEDMV 130
                ++M KG      + +DG+         RL ME   K   EK+ NSK+N V ED+ 
Sbjct: 75  FPSEELKMRKGNDIELNLSQDGTKPQGWKNRARLTMESKQKDSKEKINNSKDNSVTEDVG 134

[Close]

BLAST of CsGy3G002700 vs. TAIR 10
Match: AT4G16515.1 (Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: At5g60810 (RGF1), At1g13620 (RGF2), At2g04025 (RGF3), At3g30350 (RGF4), At5g51451 (RGF5), At4g16515 (RGF6), At3g02240 (RGF7), At2g03830 (RGF8) and At5g64770 (RGF9). )

HSP 1 Score: 49.3 bits (116), Expect = 2.8e-06
Identity = 20/24 (83.33%), Postives = 22/24 (91.67%), Query Frame = 0


Query: 106 EVGEDMVVMDYAQPHRKPPIHNEK 130
           E GE++VVMDY QPHRKPPIHNEK
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

The following BLAST results are available for this feature:

BLAST of CsGy3G002700 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VK8	3.9e-05	83.33	Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1	[more]

BLAST of CsGy3G002700 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650407.1	6.27e-87	100.00	uncharacterized protein LOC105434783 [Cucumis sativus] >KGN55873.1 hypothetical ...	[more]
XP_038905997.1	1.31e-55	76.74	uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida]	[more]
XP_038905996.1	9.11e-54	76.15	uncharacterized protein LOC120091906 isoform X1 [Benincasa hispida]	[more]
XP_022154042.1	6.05e-26	54.81	uncharacterized protein LOC111021396 [Momordica charantia]	[more]
XP_023540680.1	6.10e-24	48.87	uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo]	[more]

BLAST of CsGy3G002700 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L298	3.04e-87	100.00	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1	[more]
A0A6J1DKL1	2.93e-26	54.81	uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A6J1KZM9	2.03e-22	50.37	uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521...	[more]
M1A0Y5	4.14e-09	60.00	Uncharacterized protein OS=Solanum tuberosum OX=4113 PE=4 SV=1	[more]
A0A6S7NRQ9	5.93e-09	36.23	Uncharacterized protein OS=Lactuca saligna OX=75948 GN=LSAL_LOCUS30448 PE=4 SV=1	[more]

BLAST of CsGy3G002700 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16515.1	2.8e-06	83.33	Encodes a root meristem growth factor (RGF). Belongs to a family of functionall...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR34961	TRANSMEMBRANE PROTEIN	coord: 3..130
None	No IPR available	PANTHER	PTHR34961:SF7	SUBFAMILY NOT NAMED	coord: 3..130

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G002700.1	CsGy3G002700.1	mRNA