CsGy3G000420 (gene) Cucumber (Gy14) v2.1

Overview


Name	CsGy3G000420
Type	gene
Organism	Cucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description	bifunctional nuclease 2-like
Location	Gy14Chr3: 334910 .. 338172 (-)
RNA-Seq Expression	CsGy3G000420
Synteny	CsGy3G000420

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGATAAATTAGTGGAAATGAGAAAAAATTTAACTATCATATGCATTGAAATCTTATAAGATAGATGTGTTAGACTCTAATTTAGTTTAACTATATAGAGTCGAATATGGAAACTTTAATCTCAAAAGTTTGGAGAAAAAATTATCTTTGGAGAGAAAGAAAAGTTGGAATGGATGTGGAGGTTTGTATTGTGCCTCATCTTCCTTTCATCGTCTTTGTCCATTTCCTCAAAATCTCTTCTCTTTCAAATCAATTCAGCGGATTGAAAACAAACTCTCTCTGTAACAGCGAAAAATAAGCGCTTTTCAGGCCAACCCCACTTCACCCAACTTCCCCAACTTTCCCTTTTGTTCTTCTTCAATCTCCATTTTCTTCTTCCTTCTCTATTTTTTCCTTCTAATTCCCTCATTACAATGCTGGGTTTAGCCCCTCACTTCTGTCTTCCTACCATTTTCTTTGGATCCGCCATAGCCATGGATCACCCCTCTGCTTCTCGCCCTTCCTGTTCCCTTCACTTCTCTCTTCACCGATCGCGTCCCAATGCGGTTCGTTCTCGCCGTCCCAAGTCGATCCTCATTTCTTGTCACTCTTCTCGTGATAGATCCACCCACAGTGATGATGATGATAATTCCCATCAGGATTATTTCGAAGCTTCGTTTCTTGTTTCAGGTTTTCGGTTTTATTGTTTGGGTTTCCTTTTTTAGAGCTTATAATTGTTAATTAAATCGATTTGGGGTTGTTGGTGTTTCTGATTCCTGATTTTTTTTTTTTTTTTTGTTTCTTTCTTTTTCTACTATTAGAGACTATCTATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAATTTCAACCAGAGAGAATCTCGTGCCAATTCTAATTCACTTGGACTGGGGTTTTTTAGACGTTTTCGAAGCCCCACTGTATTTCTCAAAATCTCCTGCAATGGAGACTTTCTACTGCCAATTGTCGTTGGTATATTGGATGATGATAACTCTGTTGTGATTGGGGTTGTGTTTTCTTATGAATTTGTTGATAAGAAGTTGTAAATTTTGGACTTTTTTCGCAGGGGAATATGCTATTGAGAAACTTATTGATTGTCAGCTTGGGATTGAAAATGGGGTTGGTTTTCTTTGGTTTTGTTATCCATTTTTTTTGGTTTTGCATACTTTGTTGTTGGCATTAATTGCCAAATGACCATGTTACAGGAGGCCCCGGATATTTTTCAGTTTATACAAGACCTCATTGTGAAGGTTGGCTATGAGGTATGGAACTACCTTCTAACTTACCAAATGAATGCATGCTCAACCTTTATTATTATTTTTAGGTTCATGTGCGTGATTAAACCATTGAACTTTGGAATGATAATTGATGCTTTATCTACTGAGCTACCCTCAAATTGGCCGTAGATTTTATATTTGGCTCTGTGTTCTCTCTTGGGGAGAGTTTGAGACATGAATGTGAAGGACACTAGGATGGAATTTTCAGCTTTTGTTCATGCACTAGCTTGAGATGTAAATTGTTACTTCCTGATATTGTACATGGAAGAATCAAGAAATATGTGCACGAACTATTTGCATTTTTTTCTACACAAGAAGCAATAGGCTGAAAAAAATCTGCTAGTTTTGTTTCTTCAATATAATTGAGTATTACTTCTGACTAATGAGAATGCACGCCATGGCTTCTGGATAAATCGTTTGTACTTGTCATGTACATTTCTATTTTTCTGCTAACCATGAAATAGGGGTTAGAGCCATAATCTAATGCCACTTCTTGTTGTTATGACTTTAGGCCATCACAGCGAGAATTACTGAAAGAGTGGTGAATACTTACTTTGCCCGGCTCTTCTTGAGAAAGGTTAGCTCTTATACATTCATTCGCAATTAGAATACATGTTTAGAAGTTTTCAATACTATTATCATCTGCAGGAAGGGGAAAGTGAGATGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTCAATATTGCTTATCGGTGTAAGGTAGATATTCTACCATTAACTGCTATGGTTTTTGAAGAGCTTATAGTTGCTGGAATGACTTAGTTTCCTTTAGAACGAAGTACAGCAGTATATATGCTTTACTTTGGATCCTTTCACCCCCTTTTCATGACAGAAATAATAATTTCTCACTTCATTGAAACTTTGCTGCCATGCCTTTTTTTTTTTTTGTTTAACGAATTTGCAGATACCGGTACTTGTTAGTAAGCAGATTGTCTTTGAGGATGCGATAAGAGTTAGTTACGGGTTTGGAAGAGTGCACGAGAGGAAGTCATGTTTTGATGTTTTACTTGACTGGTATAAGTGCGAGCATTGTTGGCTTGTTTGATAGATATCTTTTCTTGTTATCTAAAAATGATGATTTCTTGTCCAGCGCTGCAGATGGCCCAGATTTCTTGTCTGAGGAACTCGATATGCTGAAGAATATGAAAATTGCCATTTATGAAGAACGGTACAAAGATGCAGGTATAAGTTAGAGAGGGCTAACCAATTTATTGCAATTGCAGGAATGGCTATTTATGAAGAACGTTTTGTTTCATGCAAATGAGGGTATTTATGATTGCTATTTGTTCCCTTCAGCCATGTGGAGGGATAAATTAACAAAGCTTCGCAAGTCGGTCCATGAGGCGTAACTTCGATATTCAAACTTCCTTTATCTGCCCTTGGACTTGTTCTTCATGTATGTAACTCTCTGTAATCCACTGCCGTACGGAGAGAGGTACCATACGGTGAAACATGTATGAACTTTTCTGATACTGCACCCTAACAGGGCGACTCTTCTGTTGGGTAGGAATTGTTCGCTCGCAGGCATCTCTTAGAACATTCAATTCAGGATTGAATGGATTAACGCTGATGGGTTGTTACACAATATTGCAAAAAATTATCCATAGTGATGGTCGGTCTGTACAGTTACAGTTAAGAGAGTATGTAAATATTACATTTGGGTAGTCAATGTGTTGTATCTCTAAGATATGAATGAGATGGCTGATATTCTTTTTCAGCTGTAAGTTCGCAGAGAATGATAACATAAAAAATGGAAGCTTTTGTATATTCCAACAAACTCTCATTTCTCTCACATACACTCTACTTTGATCCATTTTTCATTACAAACGAAGTGAGAAAGCAAATGATAGTTCATTCGTTCCGATATTGAAATTGAGTTTCGAAATTAGAAGGTAATGATAGAAAGGATGATTAAAGATTAGTGGAGAGAGTTGTTTTATATAATTGAAGATTTGCA

mRNA sequence

GTGATAAATTAGTGGAAATGAGAAAAAATTTAACTATCATATGCATTGAAATCTTATAAGATAGATGTGTTAGACTCTAATTTAGTTTAACTATATAGAGTCGAATATGGAAACTTTAATCTCAAAAGTTTGGAGAAAAAATTATCTTTGGAGAGAAAGAAAAGTTGGAATGGATGTGGAGGTTTGTATTGTGCCTCATCTTCCTTTCATCGTCTTTGTCCATTTCCTCAAAATCTCTTCTCTTTCAAATCAATTCAGCGGATTGAAAACAAACTCTCTCTGTAACAGCGAAAAATAAGCGCTTTTCAGGCCAACCCCACTTCACCCAACTTCCCCAACTTTCCCTTTTGTTCTTCTTCAATCTCCATTTTCTTCTTCCTTCTCTATTTTTTCCTTCTAATTCCCTCATTACAATGCTGGGTTTAGCCCCTCACTTCTGTCTTCCTACCATTTTCTTTGGATCCGCCATAGCCATGGATCACCCCTCTGCTTCTCGCCCTTCCTGTTCCCTTCACTTCTCTCTTCACCGATCGCGTCCCAATGCGGTTCGTTCTCGCCGTCCCAAGTCGATCCTCATTTCTTGTCACTCTTCTCGTGATAGATCCACCCACAGTGATGATGATGATAATTCCCATCAGGATTATTTCGAAGCTTCGTTTCTTGTTTCAGAGACTATCTATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAATTTCAACCAGAGAGAATCTCGTGCCAATTCTAATTCACTTGGACTGGGGTTTTTTAGACGTTTTCGAAGCCCCACTGTATTTCTCAAAATCTCCTGCAATGGAGACTTTCTACTGCCAATTGTCGTTGGGGAATATGCTATTGAGAAACTTATTGATTGTCAGCTTGGGATTGAAAATGGGGAGGCCCCGGATATTTTTCAGTTTATACAAGACCTCATTGTGAAGGTTGGCTATGAGGCCATCACAGCGAGAATTACTGAAAGAGTGGTGAATACTTACTTTGCCCGGCTCTTCTTGAGAAAGGAAGGGGAAAGTGAGATGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTCAATATTGCTTATCGGTGTAAGATACCGGTACTTGTTAGTAAGCAGATTGTCTTTGAGGATGCGATAAGAGTTAGTTACGGGTTTGGAAGAGTGCACGAGAGGAAGTCATGTTTTGATGTTTTACTTGACTGCGCTGCAGATGGCCCAGATTTCTTGTCTGAGGAACTCGATATGCTGAAGAATATGAAAATTGCCATTTATGAAGAACGGTACAAAGATGCAGCCATGTGGAGGGATAAATTAACAAAGCTTCGCAAGTCGGTCCATGAGGCGTAACTTCGATATTCAAACTTCCTTTATCTGCCCTTGGACTTGTTCTTCATGTATGTAACTCTCTGTAATCCACTGCCGTACGGAGAGAGGTACCATACGGTGAAACATGTATGAACTTTTCTGATACTGCACCCTAACAGGGCGACTCTTCTGTTGGGTAGGAATTGTTCGCTCGCAGGCATCTCTTAGAACATTCAATTCAGGATTGAATGGATTAACGCTGATGGGTTGTTACACAATATTGCAAAAAATTATCCATAGTGATGGTCGGTCTGTACAGTTACAGTTAAGAGAGTATGTAAATATTACATTTGGGTAGTCAATGTGTTGTATCTCTAAGATATGAATGAGATGGCTGATATTCTTTTTCAGCTGTAAGTTCGCAGAGAATGATAACATAAAAAATGGAAGCTTTTGTATATTCCAACAAACTCTCATTTCTCTCACATACACTCTACTTTGATCCATTTTTCATTACAAACGAAGTGAGAAAGCAAATGATAGTTCATTCGTTCCGATATTGAAATTGAGTTTCGAAATTAGAAGGTAATGATAGAAAGGATGATTAAAGATTAGTGGAGAGAGTTGTTTTATATAATTGAAGATTTGCA

Coding sequence (CDS)

ATGCTGGGTTTAGCCCCTCACTTCTGTCTTCCTACCATTTTCTTTGGATCCGCCATAGCCATGGATCACCCCTCTGCTTCTCGCCCTTCCTGTTCCCTTCACTTCTCTCTTCACCGATCGCGTCCCAATGCGGTTCGTTCTCGCCGTCCCAAGTCGATCCTCATTTCTTGTCACTCTTCTCGTGATAGATCCACCCACAGTGATGATGATGATAATTCCCATCAGGATTATTTCGAAGCTTCGTTTCTTGTTTCAGAGACTATCTATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGATGCGAATTTCAACCAGAGAGAATCTCGTGCCAATTCTAATTCACTTGGACTGGGGTTTTTTAGACGTTTTCGAAGCCCCACTGTATTTCTCAAAATCTCCTGCAATGGAGACTTTCTACTGCCAATTGTCGTTGGGGAATATGCTATTGAGAAACTTATTGATTGTCAGCTTGGGATTGAAAATGGGGAGGCCCCGGATATTTTTCAGTTTATACAAGACCTCATTGTGAAGGTTGGCTATGAGGCCATCACAGCGAGAATTACTGAAAGAGTGGTGAATACTTACTTTGCCCGGCTCTTCTTGAGAAAGGAAGGGGAAAGTGAGATGTTAAGTGTGGATGCACGCCCCTCAGACGCCCTCAATATTGCTTATCGGTGTAAGATACCGGTACTTGTTAGTAAGCAGATTGTCTTTGAGGATGCGATAAGAGTTAGTTACGGGTTTGGAAGAGTGCACGAGAGGAAGTCATGTTTTGATGTTTTACTTGACTGCGCTGCAGATGGCCCAGATTTCTTGTCTGAGGAACTCGATATGCTGAAGAATATGAAAATTGCCATTTATGAAGAACGGTACAAAGATGCAGCCATGTGGAGGGATAAATTAACAAAGCTTCGCAAGTCGGTCCATGAGGCGTAA

Protein sequence

MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSSRDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLRKSVHEA*

Homology

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BLAST of CsGy3G000420 vs. ExPASy Swiss-Prot
Match: Q93VH2 (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.0e-29
Identity = 96/300 (32.00%), Postives = 151/300 (50.33%), Query Frame = 0


Query: 14  FFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSI--LISCHSSRDRSTHSDDDD 73
           F+G+    D   A+  +  LH             RR KSI  L S HS    ST  + ++
Sbjct: 39  FWGNQTKNDKSQAATVNLRLHL------------RRYKSIKCLFSSHSDGTGSTAENFNE 98

Query: 74  NSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGFFRRFRSPTVFL 133
           N  +DY ++S L +  +   +     F       ++    ++N  G           + L
Sbjct: 99  ND-EDYVKSSVLEAVEV---KSGPDGFMVKMKDGRQLRCVHNNPQGGNLPNYAPHSAIVL 158

Query: 134 KISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITARITER 193
           K+      LLPI+V E     L+     ++    P ++Q ++D++ K+GYE    R+T R
Sbjct: 159 KMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKDMVDKMGYEVRLVRVTTR 218

Query: 194 VVNTYFARLFLRKEGE-SEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRVSYGFG 253
           V   YFA L+L K G+ S+ +S D RPSDA+NIA RCK+P+ V+K + + D +RV    G
Sbjct: 219 VHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-IDSG 278

Query: 254 RVHERKSCFDVLLDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLR 307
           ++ ++    D LL    D P+    F ++E D+++NM  A+ EERY +AA WRDKL K +
Sbjct: 279 KLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLGKFQ 320

[Close]

BLAST of CsGy3G000420 vs. ExPASy Swiss-Prot
Match: Q6YZM6 (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 6.0e-28
Identity = 77/200 (38.50%), Postives = 109/200 (54.50%), Query Frame = 0


Query: 112 NSNSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQF 171
           ++NS G         P + L+I    + LLPI+V E     L+     +     P I+Q 
Sbjct: 121 HNNSQGRNIPESAPQPAIVLRIEDGSETLLPIIVLEMPSVLLMAAIRNVHIAR-PTIYQV 180

Query: 172 IQDLIVKVGYEAITARITERVVNTYFARLFLRKEGE-SEMLSVDARPSDALNIAYRCKIP 231
           ++++I K+GYE    RI +R+   Y A LFL K G+ +E ++ D RPSDA+NIA RCK+P
Sbjct: 181 VKEMIDKMGYEVKLVRINKRIQEAYCAELFLTKVGDHTESITFDLRPSDAINIAVRCKVP 240

Query: 232 VLVSKQIVFEDAIRVSYGFGRVHERKSCFDVLLDCAADGPD----FLSEELDMLKNMKIA 291
           + V + + + D IR S    R+       D LL    D PD      ++E  +++NM IA
Sbjct: 241 IQVHRSLAYSDGIR-SVEPARMAIAAGMSDGLLFTELDRPDGQPCVEAQEFGLIRNMLIA 300

Query: 292 IYEERYKDAAMWRDKLTKLR 307
             EERYKDAA WRDKL  LR
Sbjct: 301 AVEERYKDAATWRDKLMLLR 318

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BLAST of CsGy3G000420 vs. ExPASy Swiss-Prot
Match: Q9FWS6 (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-27
Identity = 72/185 (38.92%), Postives = 106/185 (57.30%), Query Frame = 0


Query: 127 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITA 186
           P + LK+      LLPI+V E     L+     ++    P ++Q +++++ K+GYE    
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKEMVDKMGYEVRLV 193

Query: 187 RITERVVNTYFARLFLRKEGE-SEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRV 246
           R+T+RV   YFA+LFL K G  SE +S D RPSDA+NIA RCKIP+ V+K + + D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 247 SYGFGRVHERKSCFDVLLDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDK 306
               G++       D LL    D P+      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 IES-GKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

[Close]

BLAST of CsGy3G000420 vs. ExPASy Swiss-Prot
Match: B8A8D2 (Bifunctional nuclease 1 OS=Oryza sativa subsp. indica OX=39946 GN=BBD1 PE=3 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.6e-25
Identity = 69/186 (37.10%), Postives = 108/186 (58.06%), Query Frame = 0


Query: 129 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITARI 188
           + LK+    D LLPI+V E     L+     I     P I+  ++++  ++GY     RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 189 TERVVNTYFARLFLRKEG-ESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRV-- 248
           TE V + Y++RL+L K G E E +S+D +PSDA+NIA+RCK+P+ V+++I + + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 249 -----SYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 307
                SY      ++  C    LD   D P F ++E D+++NM +A  EERYKDAA +RD
Sbjct: 265 PTPSESYVSS---DQFQC--TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRD 324

[Close]

BLAST of CsGy3G000420 vs. ExPASy Swiss-Prot
Match: Q5N8J3 (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 117.9 bits (294), Expect = 2.1e-25
Identity = 67/181 (37.02%), Postives = 105/181 (58.01%), Query Frame = 0


Query: 129 VFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITARI 188
           + LK+    D LLPI+V E     L+     I     P I+  ++++  ++GY     RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 189 TERVVNTYFARLFLRKEG-ESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRVSY 248
           TE V + Y++RL+L K G E E +S+D +PSDA+NIA+RCK+P+ V+++I + + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 249 GF-GRVHERKSCFD-VLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKL 307
                 +     F    LD   D P F ++E D+++NM +A  EERYKDAA +RD+L   
Sbjct: 265 PTPSESYVSSDQFQYTRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFMF 324

[Close]

BLAST of CsGy3G000420 vs. NCBI nr
Match: XP_011650273.1 (bifunctional nuclease 2 [Cucumis sativus] >KGN55629.1 hypothetical protein Csa_011518 [Cucumis sativus])

HSP 1 Score: 626 bits (1615), Expect = 2.47e-226
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS
Sbjct: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF
Sbjct: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSVHEA 312
           KLTKLRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 312

[Close]

BLAST of CsGy3G000420 vs. NCBI nr
Match: XP_008448511.1 (PREDICTED: bifunctional nuclease 2 isoform X1 [Cucumis melo] >XP_008448512.1 PREDICTED: bifunctional nuclease 2 isoform X1 [Cucumis melo])

HSP 1 Score: 610 bits (1573), Expect = 6.23e-220
Identity = 306/312 (98.08%), Postives = 307/312 (98.40%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHF LPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS
Sbjct: 1   MLGLAPHFRLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRST+SDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQED NFNQRESR NS SLGLGF
Sbjct: 61  RDRSTNSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDVNFNQRESRVNSPSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQ LIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQGLIVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSVHEA 312
           KLTKLRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 312

[Close]

BLAST of CsGy3G000420 vs. NCBI nr
Match: XP_038905904.1 (bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nuclease 2 [Benincasa hispida] >XP_038905906.1 bifunctional nuclease 2 [Benincasa hispida])

HSP 1 Score: 580 bits (1496), Expect = 3.40e-208
Identity = 296/313 (94.57%), Postives = 300/313 (95.85%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHFC PTIF GSAIAMDHPSASRPS SLHFS+ RSRP  VRSRRPKSILISCHSS
Sbjct: 1   MLGLAPHFCFPTIFPGSAIAMDHPSASRPSSSLHFSVLRSRPIPVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRES-RANSNSLGLG 120
           RDRSTHS DDD SHQDYFEASFLVSETIYHYRMWKKRFQEDA FNQRES R NS+SLGLG
Sbjct: 61  RDRSTHSHDDD-SHQDYFEASFLVSETIYHYRMWKKRFQEDATFNQRESSRVNSHSLGLG 120

Query: 121 FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKV 180
           FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKV
Sbjct: 121 FFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKV 180

Query: 181 GYEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVF 240
           GYEA TARITERVVNTYFARLFLRKEGE+EMLSVDARPSDALNIAYRCKIPVLVSKQIVF
Sbjct: 181 GYEATTARITERVVNTYFARLFLRKEGENEMLSVDARPSDALNIAYRCKIPVLVSKQIVF 240

Query: 241 EDAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWR 300
           ED IRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWR
Sbjct: 241 EDGIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWR 300

Query: 301 DKLTKLRKSVHEA 312
           DKLTKLRKSVHEA
Sbjct: 301 DKLTKLRKSVHEA 312

[Close]

BLAST of CsGy3G000420 vs. NCBI nr
Match: KAA0052933.1 (bifunctional nuclease 2 isoform X1 [Cucumis melo var. makuwa] >TYK11390.1 bifunctional nuclease 2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 560 bits (1443), Expect = 2.01e-200
Identity = 286/312 (91.67%), Postives = 288/312 (92.31%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHF LPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS
Sbjct: 1   MLGLAPHFRLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRST                   ++TIYHYRMWKKRFQED NFNQRESR NS SLGLGF
Sbjct: 61  RDRST-------------------NKTIYHYRMWKKRFQEDVNFNQRESRVNSPSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQ LIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQGLIVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 293

Query: 301 KLTKLRKSVHEA 312
           KLTKLRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 293

[Close]

BLAST of CsGy3G000420 vs. NCBI nr
Match: KAG7015839.1 (Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 559 bits (1440), Expect = 1.08e-199
Identity = 282/312 (90.38%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHF L T+F GSAIAMDHPSASRPS SLHFS+ RSRPNAV SRRPKSIL+SCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRSTHSDDD  SHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQ ESR NS SLGLGF
Sbjct: 61  RDRSTHSDDD--SHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENGEAPD+FQFIQDL+VKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YE  TARITERVVNTY+ARLFLRK+GE+E LSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEVTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVS+GFGRV +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRD
Sbjct: 241 DAIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSVHEA 312
           KLT+LRKSVHEA
Sbjct: 301 KLTRLRKSVHEA 310

[Close]

BLAST of CsGy3G000420 vs. ExPASy TrEMBL
Match: A0A0A0L3L8 (BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV=1)

HSP 1 Score: 626 bits (1615), Expect = 1.19e-226
Identity = 312/312 (100.00%), Postives = 312/312 (100.00%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS
Sbjct: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF
Sbjct: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSVHEA 312
           KLTKLRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 312

[Close]

BLAST of CsGy3G000420 vs. ExPASy TrEMBL
Match: A0A1S3BJU6 (bifunctional nuclease 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490665 PE=3 SV=1)

HSP 1 Score: 610 bits (1573), Expect = 3.02e-220
Identity = 306/312 (98.08%), Postives = 307/312 (98.40%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHF LPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS
Sbjct: 1   MLGLAPHFRLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRST+SDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQED NFNQRESR NS SLGLGF
Sbjct: 61  RDRSTNSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDVNFNQRESRVNSPSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQ LIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQGLIVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSVHEA 312
           KLTKLRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 312

[Close]

BLAST of CsGy3G000420 vs. ExPASy TrEMBL
Match: A0A5D3CJ41 (Bifunctional nuclease 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00420 PE=3 SV=1)

HSP 1 Score: 560 bits (1443), Expect = 9.73e-201
Identity = 286/312 (91.67%), Postives = 288/312 (92.31%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHF LPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS
Sbjct: 1   MLGLAPHFRLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRST                   ++TIYHYRMWKKRFQED NFNQRESR NS SLGLGF
Sbjct: 61  RDRST-------------------NKTIYHYRMWKKRFQEDVNFNQRESRVNSPSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQ LIVKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQGLIVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 293

Query: 301 KLTKLRKSVHEA 312
           KLTKLRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 293

[Close]

BLAST of CsGy3G000420 vs. ExPASy TrEMBL
Match: A0A6J1HJX9 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV=1)

HSP 1 Score: 559 bits (1440), Expect = 5.22e-200
Identity = 282/312 (90.38%), Postives = 294/312 (94.23%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGL PHF L T+F GSAIAMDHPSASRPS SLHFS+ RSRPNAVRSRRPKSIL+SCHSS
Sbjct: 1   MLGLVPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRSTHSDDD  SHQDYFEASFLVSETIYHYRMWKKRFQED NFNQ ESR NS SLGLGF
Sbjct: 61  RDRSTHSDDD--SHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRF+SPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENGEAPD+FQFIQDL+VKVG
Sbjct: 121 FRRFQSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YE  TARITERVVNTYFARLFLRK+GE+E LSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEVTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVS+GFGRV +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRD
Sbjct: 241 DAIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSVHEA 312
           KLTKLRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 310

[Close]

BLAST of CsGy3G000420 vs. ExPASy TrEMBL
Match: A0A6J1E735 (bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 SV=1)

HSP 1 Score: 558 bits (1437), Expect = 1.50e-199
Identity = 282/312 (90.38%), Postives = 294/312 (94.23%), Query Frame = 0


Query: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60
           MLGLAPHF L T+F GSAIAMDHPSASRPS SLHFS+ RSRPNAV SRRPKSIL+SCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120
           RDRSTHSDDD  SHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQ ESR NS  LGLGF
Sbjct: 61  RDRSTHSDDD--SHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPLLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENGEAPD+FQFIQDL+VKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVG 180

Query: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240
           YE  TARITERVVNTYFARLFLRK+GE+E LSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEVTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240

Query: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300
           DAIRVS+GFGRV +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRD
Sbjct: 241 DAIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRD 300

Query: 301 KLTKLRKSVHEA 312
           KLT+LRKSVHEA
Sbjct: 301 KLTRLRKSVHEA 310

[Close]

BLAST of CsGy3G000420 vs. TAIR 10
Match: AT5G66050.1 (Wound-responsive family protein )

HSP 1 Score: 238.8 bits (608), Expect = 5.9e-63
Identity = 136/311 (43.73%), Postives = 189/311 (60.77%), Query Frame = 0


Query: 12  TIFFGSAIAMDHPSASRPS---CSLHFSLHRSRPNAVRSRRPKSILISCHSSRDRSTHSD 71
           T F  S  A    S+S PS   C    +L   R +    RRPK   I C  S    +   
Sbjct: 22  TTFSRSISASLSSSSSSPSSFRCCTPLNLKSLRSH----RRPKISHICCDGSSISGSGLG 81

Query: 72  DDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDA-----------NFNQRESRANSNSLG 131
           D D    ++ +AS LV+ET  HY+M +  F++D+           +    ESR     +G
Sbjct: 82  DPD---LEFLQASVLVAETSMHYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIG 141

Query: 132 LGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIV 191
           LGF R+F+ PT+FLKISC+GD+LLP++VG+ A+EKL+D  L     E PD FQF+  ++ 
Sbjct: 142 LGFLRQFKQPTIFLKISCDGDYLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVD 201

Query: 192 KVGYEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQI 251
           K+GYE    ++T R+VNTY+A L L K G+ E + +D+RPSDA+N+A  C+ P+ V+K I
Sbjct: 202 KLGYEVKMVKLTGRIVNTYYASLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAI 261

Query: 252 VFEDAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAM 309
           V E+AI++ YG GR    K  F+V+LD A DGPD LSEEL +++NM +A  EERY DAAM
Sbjct: 262 VLEEAIKIGYG-GRPQSAKPVFNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAM 321

[Close]

BLAST of CsGy3G000420 vs. TAIR 10
Match: AT5G66050.2 (Wound-responsive family protein )

HSP 1 Score: 223.8 bits (569), Expect = 1.9e-58
Identity = 110/230 (47.83%), Postives = 155/230 (67.39%), Query Frame = 0


Query: 90  HYRMWKKRFQEDA-----------NFNQRESRANSNSLGLGFFRRFRSPTVFLKISCNGD 149
           HY+M +  F++D+           +    ESR     +GLGF R+F+ PT+FLKISC+GD
Sbjct: 2   HYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIGLGFLRQFKQPTIFLKISCDGD 61

Query: 150 FLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITARITERVVNTYFA 209
           +LLP++VG+ A+EKL+D  L     E PD FQF+  ++ K+GYE    ++T R+VNTY+A
Sbjct: 62  YLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGYEVKMVKLTGRIVNTYYA 121

Query: 210 RLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRVSYGFGRVHERKSC 269
            L L K G+ E + +D+RPSDA+N+A  C+ P+ V+K IV E+AI++ YG GR    K  
Sbjct: 122 SLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEEAIKIGYG-GRPQSAKPV 181

Query: 270 FDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLRKS 309
           F+V+LD A DGPD LSEEL +++NM +A  EERY DAAMWRD+L  L+ S
Sbjct: 182 FNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAMWRDRLKNLQNS 230

[Close]

BLAST of CsGy3G000420 vs. TAIR 10
Match: AT1G19660.1 (Wound-responsive family protein )

HSP 1 Score: 129.4 bits (324), Expect = 5.0e-30
Identity = 96/300 (32.00%), Postives = 151/300 (50.33%), Query Frame = 0


Query: 14  FFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSI--LISCHSSRDRSTHSDDDD 73
           F+G+    D   A+  +  LH             RR KSI  L S HS    ST  + ++
Sbjct: 39  FWGNQTKNDKSQAATVNLRLHL------------RRYKSIKCLFSSHSDGTGSTAENFNE 98

Query: 74  NSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGFFRRFRSPTVFL 133
           N  +DY ++S L +  +   +     F       ++    ++N  G           + L
Sbjct: 99  ND-EDYVKSSVLEAVEV---KSGPDGFMVKMKDGRQLRCVHNNPQGGNLPNYAPHSAIVL 158

Query: 134 KISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITARITER 193
           K+      LLPI+V E     L+     ++    P ++Q ++D++ K+GYE    R+T R
Sbjct: 159 KMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKDMVDKMGYEVRLVRVTTR 218

Query: 194 VVNTYFARLFLRKEGE-SEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRVSYGFG 253
           V   YFA L+L K G+ S+ +S D RPSDA+NIA RCK+P+ V+K + + D +RV    G
Sbjct: 219 VHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-IDSG 278

Query: 254 RVHERKSCFDVLLDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLR 307
           ++ ++    D LL    D P+    F ++E D+++NM  A+ EERY +AA WRDKL K +
Sbjct: 279 KLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLGKFQ 320

[Close]

BLAST of CsGy3G000420 vs. TAIR 10
Match: AT1G19660.2 (Wound-responsive family protein )

HSP 1 Score: 129.4 bits (324), Expect = 5.0e-30
Identity = 96/300 (32.00%), Postives = 151/300 (50.33%), Query Frame = 0


Query: 14  FFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSI--LISCHSSRDRSTHSDDDD 73
           F+G+    D   A+  +  LH             RR KSI  L S HS    ST  + ++
Sbjct: 39  FWGNQTKNDKSQAATVNLRLHL------------RRYKSIKCLFSSHSDGTGSTAENFNE 98

Query: 74  NSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGFFRRFRSPTVFL 133
           N  +DY ++S L +  +   +     F       ++    ++N  G           + L
Sbjct: 99  ND-EDYVKSSVLEAVEV---KSGPDGFMVKMKDGRQLRCVHNNPQGGNLPNYAPHSAIVL 158

Query: 134 KISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITARITER 193
           K+      LLPI+V E     L+     ++    P ++Q ++D++ K+GYE    R+T R
Sbjct: 159 KMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKDMVDKMGYEVRLVRVTTR 218

Query: 194 VVNTYFARLFLRKEGE-SEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRVSYGFG 253
           V   YFA L+L K G+ S+ +S D RPSDA+NIA RCK+P+ V+K + + D +RV    G
Sbjct: 219 VHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV-IDSG 278

Query: 254 RVHERKSCFDVLLDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDKLTKLR 307
           ++ ++    D LL    D P+    F ++E D+++NM  A+ EERY +AA WRDKL K +
Sbjct: 279 KLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKLGKFQ 320

[Close]

BLAST of CsGy3G000420 vs. TAIR 10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 124.8 bits (312), Expect = 1.2e-28
Identity = 72/185 (38.92%), Postives = 106/185 (57.30%), Query Frame = 0


Query: 127 PTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVGYEAITA 186
           P + LK+      LLPI+V E     L+     ++    P ++Q +++++ K+GYE    
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKEMVDKMGYEVRLV 193

Query: 187 RITERVVNTYFARLFLRKEGE-SEMLSVDARPSDALNIAYRCKIPVLVSKQIVFEDAIRV 246
           R+T+RV   YFA+LFL K G  SE +S D RPSDA+NIA RCKIP+ V+K + + D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 247 SYGFGRVHERKSCFDVLLDCAADGPD----FLSEELDMLKNMKIAIYEERYKDAAMWRDK 306
               G++       D LL    D P+      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 IES-GKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

The following BLAST results are available for this feature:

BLAST of CsGy3G000420 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VH2	7.0e-29	32.00	Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1	[more]
Q6YZM6	6.0e-28	38.50	Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV...	[more]
Q9FWS6	1.7e-27	38.92	Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1	[more]
B8A8D2	1.6e-25	37.10	Bifunctional nuclease 1 OS=Oryza sativa subsp. indica OX=39946 GN=BBD1 PE=3 SV=1	[more]
Q5N8J3	2.1e-25	37.02	Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV...	[more]

BLAST of CsGy3G000420 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_011650273.1	2.47e-226	100.00	bifunctional nuclease 2 [Cucumis sativus] >KGN55629.1 hypothetical protein Csa_0...	[more]
XP_008448511.1	6.23e-220	98.08	PREDICTED: bifunctional nuclease 2 isoform X1 [Cucumis melo] >XP_008448512.1 PRE...	[more]
XP_038905904.1	3.40e-208	94.57	bifunctional nuclease 2 [Benincasa hispida] >XP_038905905.1 bifunctional nucleas...	[more]
KAA0052933.1	2.01e-200	91.67	bifunctional nuclease 2 isoform X1 [Cucumis melo var. makuwa] >TYK11390.1 bifunc...	[more]
KAG7015839.1	1.08e-199	90.38	Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CsGy3G000420 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0L3L8	1.19e-226	100.00	BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV...	[more]
A0A1S3BJU6	3.02e-220	98.08	bifunctional nuclease 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490665 PE=3 ...	[more]
A0A5D3CJ41	9.73e-201	91.67	Bifunctional nuclease 2 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A6J1HJX9	5.22e-200	90.38	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV...	[more]
A0A6J1E735	1.50e-199	90.38	bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 ...	[more]

BLAST of CsGy3G000420 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66050.1	5.9e-63	43.73	Wound-responsive family protein	[more]
AT5G66050.2	1.9e-58	47.83	Wound-responsive family protein	[more]
AT1G19660.1	5.0e-30	32.00	Wound-responsive family protein	[more]
AT1G19660.2	5.0e-30	32.00	Wound-responsive family protein	[more]
AT1G75380.1	1.2e-28	38.92	bifunctional nuclease in basal defense response 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003729	Bifunctional nuclease domain	PFAM	PF02577	DUF151	coord: 138..245 e-value: 1.7E-15 score: 56.8
IPR003729	Bifunctional nuclease domain	PROSITE	PS51658	BFN	coord: 108..245 score: 25.164991
IPR036104	Bifunctional nuclease superfamily	GENE3D	3.10.690.10	Bifunctional nuclease domain	coord: 104..248 e-value: 2.8E-26 score: 93.8
IPR036104	Bifunctional nuclease superfamily	SUPERFAMILY	103256	Hypothetical protein TM0160	coord: 114..247
None	No IPR available	PANTHER	PTHR15160	VON HIPPEL-LINDAU PROTEIN	coord: 43..305
None	No IPR available	PANTHER	PTHR15160:SF1	VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR-RELATED	coord: 43..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CsGy3G000420.2	CsGy3G000420.2	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030891	VCB complex
molecular_function	GO:0004518	nuclease activity