CsGy2G005904 (gene) Cucumber (Gy14) v2.1

Overview
NameCsGy2G005904
Typegene
OrganismCucumis sativus L. var. sativus cv. Gy14 (Cucumber (Gy14) v2.1)
Description(R)-mandelonitrile lyase 1-like
LocationGy14Chr2: 4185719 .. 4189231 (-)
RNA-Seq ExpressionCsGy2G005904
SyntenyCsGy2G005904
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAATTATTCTAAGGCTAACATTCTCATACTCAACCTTATAATCTCTATCTTTCAATTACAAGTTCACTCCTCGCATGCTATCCCCAATCAAGGTACTACGATTACAAATTGTTACAGAAAGTTATCGTCAGAATGAGGCTCACAGTTTTTCAACGTTTGTAACATGTCACCTTTTTTTTTTATCACATTTACCTTCATTGATACTTATTTTGGTATGAACTTTCCTTTTTCAGATGTTAGCTACATGAAGTTTGTACATAATGCTACCGATTTACCAAGAAAAGAAGAATTTGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAGTTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACATTTCGTCTCGGAGGACGGCGTAGAGAACATTCGAGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGCACATAAAGAGTTTTTTGAAAGTGCAGGTGTTGATTGGGACATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGAGTGTGGTATCACAACCGATTTTGAAAGATTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGGGGGTATTGTTCCCGATAATGGGTTTGATTTGAGGCATCTTGTAGGTACTAAAACTGGAGGCTCTATCATTGATGATAAAGGAAATAGGCATGGAGCTGTGGAGCTTCTAAATAAGGCTGATCCCAAGAATCTAAAAGTTGCAATTGAAGCCACAGTCCGAAGAATCCTCTTCTCTGGTTAGTCCTAATTAAATATAAATGTATTAGATTCAATAATTCTAAAGTAAACCTAATTAAAAACAATCTAATCATGTTTCACGATTTAAACCATTCTTCTATTAACAAAGTTTTATTTTGATAACACCATCAGTATGGATGTTTAGTATTAATAATCAATAATATATATTCATATAGATATCTATAATAACAGAAGTTTGTTATTTTTTTTTCCCTTTTATCCCCTTTTGTTTCTAGAATAATTATTCATACTTATAATCTATATCCATATCAGTCATAAATAAAAAGGTTAGGATTCTTTTAAAACAGAAGATAAAAAGATATCTATAATAACAGAAGTTTAGAATTATTAGATTCAATAATTCTAAAGTAAACCTAATTAAAAACAATCTAATCATGTTTCACGATTTAAACCATTCTTCTATTAACAAAGTTTTATTTTGATAACACCATCAGTATGGATGTTTAGTATTAATAATCAATAATATATATTCATATAGATATCTATAATAACAGAAGTTTGTTATTTTTTTTTCCCTTTTATCCCCTTTTGTTTCTAGAATAATTATTCATACTTATAATCTATATCCATATCAGTCATAAATAAAAAGGTTAGGATTCTTTTTCCTTTCATATCAATTATAAACAAAAATCTACTACCAAGTGAAACAAGTTTAAATCAGTTTCAAATTAAAGGGTCTAAATAATTTATCAACTCCACACTTTTTCACATGTATTATATTATAAACATGCAGATTTGTCAGCAAATGGAGTTTCATATTTAGATTCCAAAGGAAAGTTACACACGGCATTCATTCATGAAAAAGGAGAAATCTTTTTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGAATTGGTCCAAAATCTCATCTTTCATCCCTAAAGCTACCTGTCGTTCTTCACCAACCACATGTCGGACAATCCATGACCGACAATCCCCGTTTTGGGACAAACATTATCCTTCCATTCCAAGTGGTTCCTACATCAGGAAAAGTTGTTGGAATTTTACAAGACAATATCTATTTCCAATCTATTGCTAGCCCTTCACCATTTTTAGTTCCACCAACTTTCAGTCTTCTTCCTCCTCATCCCACTTCAATTAATCCAACCTTAGCCATTTTTTTTGGGAAATTCTCCGAAGTTCATTCTAAAGGCTCACTTAAACTAAATTCCTCTATTGATGTGAAGAAGAATCCCATTGTTCGGTTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACGTTGGAAAAGATTAAGACCCAGAATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGGAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTTGTGGACGGTAATTATAAAGTGATCGAAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACCCTCATGATGCTTGGCCGGTGAGTACTACTTTACATTTATTCACATATAACTAATAATTAGAAATTAAATTAAATTTAGTACTTATAACTAATAATTAAAATTTAGTCCTAACTTGAAGGAATGGATACATCATTAATTCATAAAGGAACTATATATTTATAAGATTTTATTAACGATATATAAGGCTTAATTTTAAATGTGGCCCTCTTGATAATTTCAACCTTAGAGAAAAAATTTGTAATTAAAATTTTTTTAATCATTTTCTAAAACTTCAATTGTTTTATTTTCCATGTATGGATTGGTGTCTACTAAGTTTTGTTCATTCACCCATTTAATATTTAAAAACTTTTACGAAGTCAATTAGATATAAAATTATTAAGTTAATGTCTTTAACAAAAGAATTGAAGAGATCTGAAATGGTAGTTTTAAAGACAACAAGACACTTTTAAACTTAAGAATTTGAATATAATATCAAATCAAGTAAAAACCAATAATTTTTGTTTTAAGAATTCAACTGTTTTATTAAGATATATAAAAATTAAAATTAAAAAATATGGAAAAAAAAATATAAAACTAAATAAGATATCTCGTAGTCAACGAAGGTTTAGAGTCTAAAATTGAAATTGTTTTTTTTTCCTCTAAATTTCTTAATATGAACCTTTCAAGGGACATTTAGAGAATAGAATTAAAAGAATTAAATGTATATTAGGGATAGTAGATCAAAGTGGTCTTTAACAGGAAAATATTTATGAAACATATAGCAAAATTTTAGACAAAGATATAAATCTATCATAGATCATTATCTATGAGTGTTTTATTTTCTATATCCGTATTTACTATTAATAGAGTCTAAAATTATGTTATATATTATAAATATTTTAAATAGTTTTGTCATTTAAAATAATTTTCTTAAAATATTGTTATGTATTATTTGAAAAGTTTAAATTTTTTTTTGTTGATCTTCTATTTCCATGAAATAACAAGTTCCAAATGGCCACGCAGGTATGTTGGCCTTAAGATTTTGCAACAAAGATCAAGTTAA

mRNA sequence

ATGGAGAATTATTCTAAGGCTAACATTCTCATACTCAACCTTATAATCTCTATCTTTCAATTACAAGTTCACTCCTCGCATGCTATCCCCAATCAAGATGTTAGCTACATGAAGTTTGTACATAATGCTACCGATTTACCAAGAAAAGAAGAATTTGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAGTTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACATTTCGTCTCGGAGGACGGCGTAGAGAACATTCGAGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGCACATAAAGAGTTTTTTGAAAGTGCAGGTGTTGATTGGGACATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGAGTGTGGTATCACAACCGATTTTGAAAGATTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGGGGGTATTGTTCCCGATAATGGGTTTGATTTGAGGCATCTTGTAGGTACTAAAACTGGAGGCTCTATCATTGATGATAAAGGAAATAGGCATGGAGCTGTGGAGCTTCTAAATAAGGCTGATCCCAAGAATCTAAAAGTTGCAATTGAAGCCACAGTCCGAAGAATCCTCTTCTCTGATTTGTCAGCAAATGGAGTTTCATATTTAGATTCCAAAGGAAAGTTACACACGGCATTCATTCATGAAAAAGGAGAAATCTTTTTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGAATTGGTCCAAAATCTCATCTTTCATCCCTAAAGCTACCTGTCGTTCTTCACCAACCACATGTCGGACAATCCATGACCGACAATCCCCGTTTTGGGACAAACATTATCCTTCCATTCCAAGTGGTTCCTACATCAGGAAAAGTTGTTGGAATTTTACAAGACAATATCTATTTCCAATCTATTGCTAGCCCTTCACCATTTTTAGTTCCACCAACTTTCAGTCTTCTTCCTCCTCATCCCACTTCAATTAATCCAACCTTAGCCATTTTTTTTGGGAAATTCTCCGAAGTTCATTCTAAAGGCTCACTTAAACTAAATTCCTCTATTGATGTGAAGAAGAATCCCATTGTTCGGTTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACGTTGGAAAAGATTAAGACCCAGAATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGGAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTTGTGGACGGTAATTATAAAGTGATCGAAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACCCTCATGATGCTTGGCCGGTATGTTGGCCTTAAGATTTTGCAACAAAGATCAAGTTAA

Coding sequence (CDS)

ATGGAGAATTATTCTAAGGCTAACATTCTCATACTCAACCTTATAATCTCTATCTTTCAATTACAAGTTCACTCCTCGCATGCTATCCCCAATCAAGATGTTAGCTACATGAAGTTTGTACATAATGCTACCGATTTACCAAGAAAAGAAGAATTTGACTACATAATTATAGGAGGTGGAACTGCAGGGTGTCCATTAGCTACAACATTATCATCAAAGTTCTCAGTTCTCCTTCTTGAAAGAGGCAGTGACCCCAACAAATATCCTTCTGTGTTGAATGAACAAGGTCTATTGAAGGCTTTTGATACAGAAGATGATGGCAAAACTCCCTTCCAACATTTCGTCTCGGAGGACGGCGTAGAGAACATTCGAGGGCGAGTCCTCGGCGGTGGAAGCATGGTCAATGCCGGGTTCTATTCAAGGGCACATAAAGAGTTTTTTGAAAGTGCAGGTGTTGATTGGGACATGGAATTGGTGGAGAAAGCTTATGAATGGGTTGAAGAGAGTGTGGTATCACAACCGATTTTGAAAGATTGGCAATCTGCTTTTAGAAGTGCTTTGTTGGAAGGGGGTATTGTTCCCGATAATGGGTTTGATTTGAGGCATCTTGTAGGTACTAAAACTGGAGGCTCTATCATTGATGATAAAGGAAATAGGCATGGAGCTGTGGAGCTTCTAAATAAGGCTGATCCCAAGAATCTAAAAGTTGCAATTGAAGCCACAGTCCGAAGAATCCTCTTCTCTGATTTGTCAGCAAATGGAGTTTCATATTTAGATTCCAAAGGAAAGTTACACACGGCATTCATTCATGAAAAAGGAGAAATCTTTTTAAGTGCAGGAGCCATTGGAAGTCCTCAACTTCTCCTTCTAAGTGGAATTGGTCCAAAATCTCATCTTTCATCCCTAAAGCTACCTGTCGTTCTTCACCAACCACATGTCGGACAATCCATGACCGACAATCCCCGTTTTGGGACAAACATTATCCTTCCATTCCAAGTGGTTCCTACATCAGGAAAAGTTGTTGGAATTTTACAAGACAATATCTATTTCCAATCTATTGCTAGCCCTTCACCATTTTTAGTTCCACCAACTTTCAGTCTTCTTCCTCCTCATCCCACTTCAATTAATCCAACCTTAGCCATTTTTTTTGGGAAATTCTCCGAAGTTCATTCTAAAGGCTCACTTAAACTAAATTCCTCTATTGATGTGAAGAAGAATCCCATTGTTCGGTTCAATTATTATTCCCATCCTGATGATCTTGCTCGATGTGTTAAAGGTGTAAGAAAATTGGGAGATGTGTTTAAAACTCCAACGTTGGAAAAGATTAAGACCCAGAATTTGAAGGGTAATAAAGGATTTATGTTTTTGGGGCTTCCATTGCCGGGAAATTTGTGGAATGATAGCGCTGTTGAAGAATATTGTAAGAAAACGGTGGCTACTTATTGGCATTATCATGGAGGATGTTTGGTCGGAAAAGTTGTGGACGGTAATTATAAAGTGATCGAAACTGAAAATTTACGTGTAGTTGATGGCTCCACTTTCTCTGTCTCGCCAGGAACTAATCCTATGGCCACCCTCATGATGCTTGGCCGGTATGTTGGCCTTAAGATTTTGCAACAAAGATCAAGTTAA

Protein sequence

MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFLVPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQRSS*
Homology
BLAST of CsGy2G005904 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 558.1 bits (1437), Expect = 1.1e-157
Identity = 276/542 (50.92%), Postives = 378/542 (69.74%), Query Frame = 0

Query: 7   ANILILNLIISIFQL-QVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGGTAGCP 66
           A +L+L+L + + Q  +VHS     N D SY++F ++ATDL  +  +DY+I+GGGT+GCP
Sbjct: 8   AILLVLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCP 67

Query: 67  LATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVENIRG 126
           LA TLS K+ VL+LERGS P  YP+VL   G +     EDDGKTP + FVSEDG++N+RG
Sbjct: 68  LAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRG 127

Query: 127 RVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSAFRS 186
           RVLGG SM+NAG Y+RA+   + ++GVDWDM+LV K YEWVE+++V +P  + WQS   +
Sbjct: 128 RVLGGTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGT 187

Query: 187 ALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATVRRI 246
           A LE G+ P++GF L H  GT+  GS  D+KG RH A ELLNK +  NL+V + A+V +I
Sbjct: 188 AFLEAGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKI 247

Query: 247 LFSD---LSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLSSL 306
           +FS+   L+A GV Y DS G  H AF+  KGE+ +SAG IG+PQLLLLSG+GP+S+LSSL
Sbjct: 248 IFSNAPGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSL 307

Query: 307 KLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFLVP 366
            +PVVL  P+VGQ + DNPR   NI+ P  + PT   V+GI  D  ++Q   S  PF  P
Sbjct: 308 NIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLPFTTP 367

Query: 367 PTFSLLPPHPTSI-NPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDLA 426
           P FS  P     + N T A F  K +   S GSL L SS +V+ +P V+FNYYS+P DL+
Sbjct: 368 P-FSFFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLS 427

Query: 427 RCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATY 486
            CV G++K+G++  T  L+  K ++L G +GF  LG+PLP +  +D+A E +C+++VA+Y
Sbjct: 428 HCVSGMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASY 487

Query: 487 WHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQR 544
           WHYHGGCLVGKV+DG+++V   + LRVVDGSTF  +P ++P    +MLGRYVG+KILQ+R
Sbjct: 488 WHYHGGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQER 546

BLAST of CsGy2G005904 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 544.7 bits (1402), Expect = 1.2e-153
Identity = 272/546 (49.82%), Postives = 374/546 (68.50%), Query Frame = 0

Query: 5   SKANILILNLIISIFQLQ---VHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGGT 64
           S  + ++L L I +  LQ   VHS     + D SY+ F ++ATDL  +  +DY+I+GGGT
Sbjct: 4   STMSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGT 63

Query: 65  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVE 124
           +GCPLA TLS K+ VL+LERGS P  YP+VL   G +     EDDGKTP + FVSEDG++
Sbjct: 64  SGCPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGID 123

Query: 125 NIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQS 184
           N+RGRVLGG S++NAG Y+RA+   + ++GVDWDM+LV + YEWVE+++V +P  + WQS
Sbjct: 124 NVRGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQS 183

Query: 185 AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEAT 244
             ++A LE G+ P++GF L H  GT+  GS  D+KG RH A ELLNK +  NL+V + A+
Sbjct: 184 VTKTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHAS 243

Query: 245 VRRILFSD---LSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSH 304
           V +I+FS+   L+A GV Y DS G  H AF+  KGE+ +SAG IG+PQLLLLSG+GP+S+
Sbjct: 244 VEKIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESY 303

Query: 305 LSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSP 364
           LSSL +PVVL  P+VGQ + DNPR   NI+ P  + PT   V+GI  D  ++Q   S  P
Sbjct: 304 LSSLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGISND--FYQCSFSSLP 363

Query: 365 FLVPPTFSLLPPHPTSI-NPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHP 424
           F  PP F   P     + N T A F  K +   S GSL L SS +V+ +P V+FNYYS+ 
Sbjct: 364 FTTPP-FGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNL 423

Query: 425 DDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKT 484
            DL+ CV G++K+G++  T  L+  K ++L G +GF  LG+PLP +  +D+A E +C+++
Sbjct: 424 TDLSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRES 483

Query: 485 VATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKI 544
           VA+YWHYHGGCLVGKV+DG+++V     LRVVDGSTF  +P ++P    +MLGRYVG+KI
Sbjct: 484 VASYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKI 543

BLAST of CsGy2G005904 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 7.4e-151
Identity = 270/547 (49.36%), Postives = 368/547 (67.28%), Query Frame = 0

Query: 5   SKANILILNLIISIFQLQVHSSHAIPN---QDVSYMKFVHNATDLPRKEEFDYIIIGGGT 64
           S  + ++L L I +  LQ     ++ N    D SY+ FV++ATD   +  +DYII+GGGT
Sbjct: 4   STMSAVLLVLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGT 63

Query: 65  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVE 124
           AGCPLA TLS+ +SVL+LERGS P +YP++L   G +     EDDGKTP + FVSEDG++
Sbjct: 64  AGCPLAATLSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGID 123

Query: 125 NIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQS 184
           N+RGRVLGG SM+NAG Y RA+  FF   G++WDM+LV + YEWVE+++V +P  + WQ+
Sbjct: 124 NVRGRVLGGTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQT 183

Query: 185 AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEAT 244
              +A LE GI+P+NGF + HL GT+  GS  D+ G RH + ELLNK DP NL+VA++A 
Sbjct: 184 VIGTAYLEAGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAA 243

Query: 245 VRRILFSD----LSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKS 304
           V +I+FS     ++A GV Y DS G  H AF+  +GE+ LSAG IGSPQLLLLSG+GP+S
Sbjct: 244 VEKIIFSSNTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPES 303

Query: 305 HLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPS 364
           +L+SL + VV   P+VGQ + DNPR   NI+ P  +  ++  V+GI  D  ++Q   S  
Sbjct: 304 YLTSLNISVVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSL 363

Query: 365 PFLVPPTFSLLPPHPTSI-NPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSH 424
           PF  PP FS  P     + N T A    K     S G++ LNSS DV+  P V+FNYYS+
Sbjct: 364 PFDTPP-FSFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSN 423

Query: 425 PDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKK 484
             DL+ CV G++KLG+V  T  LE  K ++L G  GF  LG+PLP N  +D+A E +C++
Sbjct: 424 LTDLSHCVSGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRE 483

Query: 485 TVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLK 544
           +VA+YWHYHGGCLVGKV+D  ++V     LRVVDGSTF  +P ++P    +MLGRY+G++
Sbjct: 484 SVASYWHYHGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQ 543

BLAST of CsGy2G005904 vs. ExPASy Swiss-Prot
Match: O82784 ((R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.6e-148
Identity = 268/546 (49.08%), Postives = 363/546 (66.48%), Query Frame = 0

Query: 7   ANILILNLIISIFQLQVHSSHAIPN----QDVSYMKFVHNATDLPRKEEFDYIIIGGGTA 66
           A +L+LNL+  +  LQ    H++ N     D  Y+KFV+NA DL  +  +DYII+GGGT+
Sbjct: 8   AVVLVLNLL--VLHLQYSEVHSLANTSSEHDFGYLKFVYNAVDLELEGSYDYIIVGGGTS 67

Query: 67  GCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVEN 126
           GCPLA TLS+ +SVL+LERG+   +YP+ L   G       +DDGKTP + FVSEDG++N
Sbjct: 68  GCPLAATLSANYSVLVLERGTIATEYPNTLTVDGFAYNLQQQDDGKTPVERFVSEDGIDN 127

Query: 127 IRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSA 186
           +R R+LGG +++NAG Y+RA++ F+ ++GV+WD++LV +AYEWVE+++V +P  + WQS 
Sbjct: 128 VRSRILGGTTIINAGVYARANESFYNNSGVEWDLDLVNEAYEWVEDAIVYKPSNQSWQSI 187

Query: 187 FRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATV 246
             +A LE G+ PDNGF L H  GT+  GS  D+ G RH + ELLNK DP NLKVA+EA V
Sbjct: 188 TGTAFLEAGVHPDNGFGLVHEEGTRLTGSTFDNSGTRHASDELLNKGDPDNLKVAVEAAV 247

Query: 247 RRILF----SDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSH 306
           ++I+F    S L+A GV Y DS G  H A +  KGE+ LSAG +G+PQLLLLSG+GP+S+
Sbjct: 248 QKIIFSTESSGLTAVGVVYTDSNGTSHRALVSGKGEVILSAGTLGTPQLLLLSGVGPESY 307

Query: 307 LSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSP 366
           L+SL + VV   P+VGQ + DNPR   NI+ P  + P++  V+GI  D  ++Q   S  P
Sbjct: 308 LTSLNISVVASHPYVGQYVNDNPRNFINILPPNPIEPSTVTVLGITSD--FYQCSLSSLP 367

Query: 367 FLVPPTFSLLPPHPTSI-NPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHP 426
           F  PP FSL P     + N T A    K     S GSL L SS +V   P V+FNY S P
Sbjct: 368 FDTPP-FSLFPTTSYPLPNQTFAHIVSKVPGPLSAGSLTLQSSSNVSVAPNVKFNYCSDP 427

Query: 427 DDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKT 486
            DL  CV G++K+G    T  L+  K  +L G  GF  LG PLP N  +D+A E++C+ T
Sbjct: 428 VDLTHCVSGMKKIGVFLSTDALKPYKVDDLPGIDGFNILGTPLPENQTDDAAFEKFCRDT 487

Query: 487 VATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKI 544
           VA+YWHYHGG +VGKV+DGN++V     LRVVDGSTF  +P ++P    +MLGRYVG KI
Sbjct: 488 VASYWHYHGGAIVGKVIDGNFRVTGINALRVVDGSTFPATPASHPQGFYLMLGRYVGTKI 547

BLAST of CsGy2G005904 vs. ExPASy Swiss-Prot
Match: O24243 ((R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 522.3 bits (1344), Expect = 6.5e-147
Identity = 265/546 (48.53%), Postives = 359/546 (65.75%), Query Frame = 0

Query: 5   SKANILILNLIISIFQLQVHSSHAIPN---QDVSYMKFVHNATDLPRKEEFDYIIIGGGT 64
           S  ++++  L + +  LQ    H++ N    D SY+KFV+NATD   +  +DYI+IGGGT
Sbjct: 4   STMSVILFVLHLLVLHLQYSEVHSLANTSAHDFSYLKFVYNATDTSLEGSYDYIVIGGGT 63

Query: 65  AGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVE 124
           +GCPLA TLS K+ VLLLERG+   +YP+ L   G       +DDGKTP + FVSEDG++
Sbjct: 64  SGCPLAATLSEKYKVLLLERGTIATEYPNTLTADGFAYNLQQQDDGKTPVERFVSEDGID 123

Query: 125 NIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQS 184
           N+R R+LGG +++NAG Y+RA+  F+   G++WD++LV K YEWVE+++V +P  + WQS
Sbjct: 124 NVRARILGGTTIINAGVYARANISFYSQTGIEWDLDLVNKTYEWVEDAIVVKPNNQSWQS 183

Query: 185 AFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEAT 244
                 LE GI+PDNGF L H  GT+  GS  D+ G RH A ELLNK DP NL VA++A+
Sbjct: 184 VIGEGFLEAGILPDNGFSLDHEAGTRLTGSTFDNNGTRHAADELLNKGDPNNLLVAVQAS 243

Query: 245 VRRILF----SDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKS 304
           V +ILF    S+LSA GV Y DS G  H AF+   GE+ +SAG IG+PQLLLLSG+GP+S
Sbjct: 244 VEKILFSSNTSNLSAIGVIYTDSDGNSHQAFVRGNGEVIVSAGTIGTPQLLLLSGVGPES 303

Query: 305 HLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPS 364
           +LSSL + VV   P+VGQ + +NPR   N   P  +  +   V+GI  D  Y+Q   S  
Sbjct: 304 YLSSLNITVVQPNPYVGQFLYNNPRNFINNFPPNPIEASVVTVLGIRSD--YYQVSLSSL 363

Query: 365 PFLVPPTFSLLPPHPTSI-NPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSH 424
           PF  PP FSL P     + N T A    +     S GS+ LNSS DV+  P ++FNYYS+
Sbjct: 364 PFSTPP-FSLFPTTSYPLPNSTFAHIVSQVPGPLSHGSVTLNSSSDVRIAPNIKFNYYSN 423

Query: 425 PDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKK 484
             DLA CV G++KLGD+ +T  LE  K +++ G  GF +LG+PLP N  +D++ E +C  
Sbjct: 424 STDLANCVSGMKKLGDLLRTKALEPYKARDVLGIDGFNYLGVPLPENQTDDASFETFCLD 483

Query: 485 TVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLK 543
            VA+YWHYHGG LVGKV+D +++V+  + LRVVD STF   P ++P    +MLGRYVGL+
Sbjct: 484 NVASYWHYHGGSLVGKVLDDSFRVMGIKALRVVDASTFPYEPNSHPQGFYLMLGRYVGLQ 543

BLAST of CsGy2G005904 vs. NCBI nr
Match: XP_031736583.1 ((R)-mandelonitrile lyase 1 [Cucumis sativus] >KAE8651658.1 hypothetical protein Csa_021303 [Cucumis sativus])

HSP 1 Score: 1089 bits (2817), Expect = 0.0
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60
           MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG
Sbjct: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180
           ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ
Sbjct: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180

Query: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240
           SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA
Sbjct: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240

Query: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300
           TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS
Sbjct: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360
           SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL
Sbjct: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDL 420
           VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDL
Sbjct: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDL 420

Query: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480
           ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQ 540
           YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQ
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQ 540

Query: 541 RSS 543
           RSS
Sbjct: 541 RSS 543

BLAST of CsGy2G005904 vs. NCBI nr
Match: XP_011650118.2 ((R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61134.2 hypothetical protein Csa_021306 [Cucumis sativus])

HSP 1 Score: 1040 bits (2688), Expect = 0.0
Identity = 512/543 (94.29%), Postives = 531/543 (97.79%), Query Frame = 0

Query: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60
           MENYSKA ILI++LIISIFQLQVH SHAIPNQDVSYMKFVHNATDLP KEE+DYIIIGGG
Sbjct: 1   MENYSKATILIVSLIISIFQLQVHFSHAIPNQDVSYMKFVHNATDLPTKEEYDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNE+GL KAFDTEDDGKTPFQ FVSEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEKGLSKAFDTEDDGKTPFQRFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180
           ENIRGR+LGGGSMVNAGFYSR HKEFFESAGVDWDMELVEKAYEW+EESVVSQPILKDWQ
Sbjct: 121 ENIRGRILGGGSMVNAGFYSRGHKEFFESAGVDWDMELVEKAYEWIEESVVSQPILKDWQ 180

Query: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240
           SAFRSALLEGGIVPDNGFDL HLVGTKTGGSIIDDKGNRHGAVELLNKA+PKNLKVAIEA
Sbjct: 181 SAFRSALLEGGIVPDNGFDLSHLVGTKTGGSIIDDKGNRHGAVELLNKANPKNLKVAIEA 240

Query: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300
           TV+RILFSDLSANGVSYLDSKGKLHT FIHEKGEI +SAGAIGSPQLLLLSGIGPKSHLS
Sbjct: 241 TVQRILFSDLSANGVSYLDSKGKLHTTFIHEKGEIIVSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360
           SLKLPVVLHQPHVGQSM+DNPRFGTNI+LPFQVVPTSGKVVGILQDNIYFQS+ASPSPFL
Sbjct: 301 SLKLPVVLHQPHVGQSMSDNPRFGTNIVLPFQVVPTSGKVVGILQDNIYFQSLASPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDL 420
           VPPTFSLLPPHPTSINPTLA FFGKFSEVHSKGSLKLNSSIDVKK+PIV+FNYYSHPDDL
Sbjct: 361 VPPTFSLLPPHPTSINPTLATFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420

Query: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480
           ARCVKGVRKLGDVFKTPT+EKIKTQ+LKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKGVRKLGDVFKTPTMEKIKTQDLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQ 540
           YWHYHGGCLVGKVVDGNYKVI TENLRVVDGSTFS+SPGTNPMAT+MMLGRYVGLKI+QQ
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGTENLRVVDGSTFSISPGTNPMATVMMLGRYVGLKIVQQ 540

Query: 541 RSS 543
            SS
Sbjct: 541 SSS 543

BLAST of CsGy2G005904 vs. NCBI nr
Match: XP_008451990.1 (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X1 [Cucumis melo])

HSP 1 Score: 976 bits (2522), Expect = 0.0
Identity = 486/543 (89.50%), Postives = 513/543 (94.48%), Query Frame = 0

Query: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60
           MENYSK+ ILIL+LIISIFQLQVHSSHAIPNQ+VSYMKFVHN  DLP KE++DYIIIGGG
Sbjct: 1   MENYSKSTILILSLIISIFQLQVHSSHAIPNQNVSYMKFVHNGIDLPAKEKYDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGL KAF TEDDGKTPFQ FVSEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180
           ENIRGRVLGGGSMVN GFYSR HKEFFESAGVDWDMELVEKAYEWVEE+VVSQPILKDWQ
Sbjct: 121 ENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180

Query: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240
           +AFRSALLEGGIVPDNGFDLRHLVGTKTGGSI D+KGNRHGAVELLNKA+PKNLKVAIE 
Sbjct: 181 AAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIEV 240

Query: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300
           TV+RILFSDLSA GVSYLDS GKLHTAFIH+KGEI LSAGAIGSPQLLLLSGIGPKSHLS
Sbjct: 241 TVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360
           SLKLPVVLHQP+VG+SM+DNPRFG ++++ FQVVPT+ KVVGILQDNIYFQS+A PSPFL
Sbjct: 301 SLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDL 420
           VPPTFSLLP  PTSIN TLA F GKFSEV+SKGSLKLNSSIDVKK+PIV+FNYYSHP DL
Sbjct: 361 VPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPGDL 420

Query: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480
           ARCVK VRKLGDVFKTPT+EKIKTQ+L+GNK FMFLGLPLP NLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQ 540
           YWHYHGGCLVGKVVDGNYKVI  ENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKI+QQ
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQ 540

Query: 541 RSS 543
           RSS
Sbjct: 541 RSS 543

BLAST of CsGy2G005904 vs. NCBI nr
Match: XP_008452005.2 (PREDICTED: (R)-mandelonitrile lyase 1-like isoform X2 [Cucumis melo])

HSP 1 Score: 923 bits (2386), Expect = 0.0
Identity = 457/511 (89.43%), Postives = 482/511 (94.32%), Query Frame = 0

Query: 33  DVSYMKFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVL 92
           +VSYMKFVHN  DLP KE++DYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYP VL
Sbjct: 33  NVSYMKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVL 92

Query: 93  NEQGLLKAFDTEDDGKTPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKEFFESAGV 152
           NEQGL KAF TEDDGKTPFQ FVSEDGVENIRGRVLGGGSMVN GFYSR HKEFFESAGV
Sbjct: 93  NEQGLSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGV 152

Query: 153 DWDMELVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSI 212
           DWDMELVEKAYEWVEE+VVSQPILKDWQ+AFRSALLEGGIVPDNGFDLRHLVGTKTGGSI
Sbjct: 153 DWDMELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSI 212

Query: 213 IDDKGNRHGAVELLNKADPKNLKVAIEATVRRILFSDLSANGVSYLDSKGKLHTAFIHEK 272
            D+KGNRHGAVELLNKA+PKNLKVAIE TV+RILFSDLSA GVSYLDS GKLHTAFIH+K
Sbjct: 213 FDNKGNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKK 272

Query: 273 GEIFLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQ 332
           GEI LSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQP+VG+SM+DNPRFG ++++ FQ
Sbjct: 273 GEIVLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQ 332

Query: 333 VVPTSGKVVGILQDNIYFQSIASPSPFLVPPTFSLLPPHPTSINPTLAIFFGKFSEVHSK 392
           VVPT+ KVVGILQDNIYFQS+A PSPFLVPPTFSLLP  PTSIN TLA F GKFSEV+SK
Sbjct: 333 VVPTAAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSK 392

Query: 393 GSLKLNSSIDVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKG 452
           GSLKLNSSIDVKK+PIV+FNYYSHP DLARCVK VRKLGDVFKTPT+EKIKTQ+L+GNK 
Sbjct: 393 GSLKLNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKR 452

Query: 453 FMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGS 512
           FMFLGLPLP NLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVI  ENLRVVDGS
Sbjct: 453 FMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGS 512

Query: 513 TFSVSPGTNPMATLMMLGRYVGLKILQQRSS 543
           TFSVSPGTNPMATLMMLGRYVGLKI+QQRSS
Sbjct: 513 TFSVSPGTNPMATLMMLGRYVGLKIVQQRSS 543

BLAST of CsGy2G005904 vs. NCBI nr
Match: XP_011650116.2 ((R)-mandelonitrile lyase 1 [Cucumis sativus])

HSP 1 Score: 920 bits (2378), Expect = 0.0
Identity = 455/540 (84.26%), Postives = 494/540 (91.48%), Query Frame = 0

Query: 4   YSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGGTAG 63
           +S+  ILIL+L+ISIFQLQ+HSSHAIPNQDVSYMKFVHNATDLP KEE+DYIIIGGGTAG
Sbjct: 3   HSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAG 62

Query: 64  CPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVENI 123
           CPLATTLSSKFSVLLLERGSDPNKYPSVL+EQGLL  F   DDG+ PFQ FVSEDGVENI
Sbjct: 63  CPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENI 122

Query: 124 RGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSAF 183
           RGRVLGGGSMVNAGFYSR H+EFF SAGVDWDMELVEKAYEWVEE+VVSQPIL  WQSAF
Sbjct: 123 RGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAF 182

Query: 184 RSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATVR 243
           RS+LLEGG+VPDNGFDLRHLVGTKTGGSI D+KGNRHGAVELLNKA+P N+KVAIEATV+
Sbjct: 183 RSSLLEGGVVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQ 242

Query: 244 RILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLSSLK 303
           RILFS LSANGV Y DSKGKLH A I +KGEI +SAGAIGSPQLLLLSGIGPKSHLSSLK
Sbjct: 243 RILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLK 302

Query: 304 LPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFLVPP 363
           LPVVLHQP+VGQSM+DNPRFGTNII+PF V+P+S KVVGILQDNIY QSIASP P L+P 
Sbjct: 303 LPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQ 362

Query: 364 TFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDLARC 423
            FSLLPP  TSI PTLA+F GKFSEVHS+GSL+LNSS +VKK+PIV FNYYSHPDDL RC
Sbjct: 363 IFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRC 422

Query: 424 VKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWH 483
           VKGVRK+GD+ KT T+EKIKT+NL+GNKGF FLG+PLP NLWNDS+VEEYCKKTV TYWH
Sbjct: 423 VKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWH 482

Query: 484 YHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQRSS 543
           YHGGCLVGKVVDGNYKVI  +NLRVVDGSTFS SPGTNPMATLMMLGRYVGLK+L QRS+
Sbjct: 483 YHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGRYVGLKLLHQRSN 542

BLAST of CsGy2G005904 vs. ExPASy TrEMBL
Match: A0A0A0LZU8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627390 PE=3 SV=1)

HSP 1 Score: 1063 bits (2749), Expect = 0.0
Identity = 529/531 (99.62%), Postives = 531/531 (100.00%), Query Frame = 0

Query: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60
           MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG
Sbjct: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180
           ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ
Sbjct: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180

Query: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240
           SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA
Sbjct: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240

Query: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300
           TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS
Sbjct: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360
           SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL
Sbjct: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDL 420
           VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKK+PIV+FNYYSHPDDL
Sbjct: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKSPIVQFNYYSHPDDL 420

Query: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480
           ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGR 531
           YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGR
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGR 531

BLAST of CsGy2G005904 vs. ExPASy TrEMBL
Match: A0A1S3BSR3 ((R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 976 bits (2522), Expect = 0.0
Identity = 486/543 (89.50%), Postives = 513/543 (94.48%), Query Frame = 0

Query: 1   MENYSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGG 60
           MENYSK+ ILIL+LIISIFQLQVHSSHAIPNQ+VSYMKFVHN  DLP KE++DYIIIGGG
Sbjct: 1   MENYSKSTILILSLIISIFQLQVHSSHAIPNQNVSYMKFVHNGIDLPAKEKYDYIIIGGG 60

Query: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGV 120
           TAGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQGL KAF TEDDGKTPFQ FVSEDGV
Sbjct: 61  TAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQGLSKAFGTEDDGKTPFQRFVSEDGV 120

Query: 121 ENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQ 180
           ENIRGRVLGGGSMVN GFYSR HKEFFESAGVDWDMELVEKAYEWVEE+VVSQPILKDWQ
Sbjct: 121 ENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDMELVEKAYEWVEETVVSQPILKDWQ 180

Query: 181 SAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEA 240
           +AFRSALLEGGIVPDNGFDLRHLVGTKTGGSI D+KGNRHGAVELLNKA+PKNLKVAIE 
Sbjct: 181 AAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPKNLKVAIEV 240

Query: 241 TVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLS 300
           TV+RILFSDLSA GVSYLDS GKLHTAFIH+KGEI LSAGAIGSPQLLLLSGIGPKSHLS
Sbjct: 241 TVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIVLSAGAIGSPQLLLLSGIGPKSHLS 300

Query: 301 SLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFL 360
           SLKLPVVLHQP+VG+SM+DNPRFG ++++ FQVVPT+ KVVGILQDNIYFQS+A PSPFL
Sbjct: 301 SLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPTAAKVVGILQDNIYFQSLAGPSPFL 360

Query: 361 VPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDL 420
           VPPTFSLLP  PTSIN TLA F GKFSEV+SKGSLKLNSSIDVKK+PIV+FNYYSHP DL
Sbjct: 361 VPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLKLNSSIDVKKSPIVQFNYYSHPGDL 420

Query: 421 ARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 480
           ARCVK VRKLGDVFKTPT+EKIKTQ+L+GNK FMFLGLPLP NLWNDSAVEEYCKKTVAT
Sbjct: 421 ARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFLGLPLPENLWNDSAVEEYCKKTVAT 480

Query: 481 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQ 540
           YWHYHGGCLVGKVVDGNYKVI  ENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKI+QQ
Sbjct: 481 YWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKIVQQ 540

Query: 541 RSS 543
           RSS
Sbjct: 541 RSS 543

BLAST of CsGy2G005904 vs. ExPASy TrEMBL
Match: A0A1S3BSS8 ((R)-mandelonitrile lyase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 923 bits (2386), Expect = 0.0
Identity = 457/511 (89.43%), Postives = 482/511 (94.32%), Query Frame = 0

Query: 33  DVSYMKFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVL 92
           +VSYMKFVHN  DLP KE++DYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYP VL
Sbjct: 33  NVSYMKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVL 92

Query: 93  NEQGLLKAFDTEDDGKTPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKEFFESAGV 152
           NEQGL KAF TEDDGKTPFQ FVSEDGVENIRGRVLGGGSMVN GFYSR HKEFFESAGV
Sbjct: 93  NEQGLSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGV 152

Query: 153 DWDMELVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSI 212
           DWDMELVEKAYEWVEE+VVSQPILKDWQ+AFRSALLEGGIVPDNGFDLRHLVGTKTGGSI
Sbjct: 153 DWDMELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSI 212

Query: 213 IDDKGNRHGAVELLNKADPKNLKVAIEATVRRILFSDLSANGVSYLDSKGKLHTAFIHEK 272
            D+KGNRHGAVELLNKA+PKNLKVAIE TV+RILFSDLSA GVSYLDS GKLHTAFIH+K
Sbjct: 213 FDNKGNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKK 272

Query: 273 GEIFLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQ 332
           GEI LSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQP+VG+SM+DNPRFG ++++ FQ
Sbjct: 273 GEIVLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQ 332

Query: 333 VVPTSGKVVGILQDNIYFQSIASPSPFLVPPTFSLLPPHPTSINPTLAIFFGKFSEVHSK 392
           VVPT+ KVVGILQDNIYFQS+A PSPFLVPPTFSLLP  PTSIN TLA F GKFSEV+SK
Sbjct: 333 VVPTAAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSK 392

Query: 393 GSLKLNSSIDVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKG 452
           GSLKLNSSIDVKK+PIV+FNYYSHP DLARCVK VRKLGDVFKTPT+EKIKTQ+L+GNK 
Sbjct: 393 GSLKLNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKR 452

Query: 453 FMFLGLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGS 512
           FMFLGLPLP NLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVI  ENLRVVDGS
Sbjct: 453 FMFLGLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGS 512

Query: 513 TFSVSPGTNPMATLMMLGRYVGLKILQQRSS 543
           TFSVSPGTNPMATLMMLGRYVGLKI+QQRSS
Sbjct: 513 TFSVSPGTNPMATLMMLGRYVGLKIVQQRSS 543

BLAST of CsGy2G005904 vs. ExPASy TrEMBL
Match: A0A1S3BTY8 ((R)-mandelonitrile lyase 1-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC103493130 PE=3 SV=1)

HSP 1 Score: 917 bits (2370), Expect = 0.0
Identity = 454/507 (89.55%), Postives = 478/507 (94.28%), Query Frame = 0

Query: 37  MKFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQG 96
           MKFVHN  DLP KE++DYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYP VLNEQG
Sbjct: 1   MKFVHNGIDLPAKEKYDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPYVLNEQG 60

Query: 97  LLKAFDTEDDGKTPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDM 156
           L KAF TEDDGKTPFQ FVSEDGVENIRGRVLGGGSMVN GFYSR HKEFFESAGVDWDM
Sbjct: 61  LSKAFGTEDDGKTPFQRFVSEDGVENIRGRVLGGGSMVNIGFYSRGHKEFFESAGVDWDM 120

Query: 157 ELVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDK 216
           ELVEKAYEWVEE+VVSQPILKDWQ+AFRSALLEGGIVPDNGFDLRHLVGTKTGGSI D+K
Sbjct: 121 ELVEKAYEWVEETVVSQPILKDWQAAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIFDNK 180

Query: 217 GNRHGAVELLNKADPKNLKVAIEATVRRILFSDLSANGVSYLDSKGKLHTAFIHEKGEIF 276
           GNRHGAVELLNKA+PKNLKVAIE TV+RILFSDLSA GVSYLDS GKLHTAFIH+KGEI 
Sbjct: 181 GNRHGAVELLNKANPKNLKVAIEVTVQRILFSDLSAKGVSYLDSTGKLHTAFIHKKGEIV 240

Query: 277 LSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPT 336
           LSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQP+VG+SM+DNPRFG ++++ FQVVPT
Sbjct: 241 LSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPYVGKSMSDNPRFGASMVVQFQVVPT 300

Query: 337 SGKVVGILQDNIYFQSIASPSPFLVPPTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLK 396
           + KVVGILQDNIYFQS+A PSPFLVPPTFSLLP  PTSIN TLA F GKFSEV+SKGSLK
Sbjct: 301 AAKVVGILQDNIYFQSLAGPSPFLVPPTFSLLPSQPTSINLTLANFVGKFSEVNSKGSLK 360

Query: 397 LNSSIDVKKNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFL 456
           LNSSIDVKK+PIV+FNYYSHP DLARCVK VRKLGDVFKTPT+EKIKTQ+L+GNK FMFL
Sbjct: 361 LNSSIDVKKSPIVQFNYYSHPGDLARCVKSVRKLGDVFKTPTMEKIKTQDLEGNKRFMFL 420

Query: 457 GLPLPGNLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSV 516
           GLPLP NLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVI  ENLRVVDGSTFSV
Sbjct: 421 GLPLPENLWNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIGIENLRVVDGSTFSV 480

Query: 517 SPGTNPMATLMMLGRYVGLKILQQRSS 543
           SPGTNPMATLMMLGRYVGLKI+QQRSS
Sbjct: 481 SPGTNPMATLMMLGRYVGLKIVQQRSS 507

BLAST of CsGy2G005904 vs. ExPASy TrEMBL
Match: A0A0A0LK76 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1)

HSP 1 Score: 899 bits (2324), Expect = 0.0
Identity = 445/528 (84.28%), Postives = 483/528 (91.48%), Query Frame = 0

Query: 4   YSKANILILNLIISIFQLQVHSSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGGTAG 63
           +S+  ILIL+L+ISIFQLQ+HSSHAIPNQDVSYMKFVHNATDLP KEE+DYIIIGGGTAG
Sbjct: 3   HSRVTILILSLMISIFQLQLHSSHAIPNQDVSYMKFVHNATDLPPKEEYDYIIIGGGTAG 62

Query: 64  CPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVENI 123
           CPLATTLSSKFSVLLLERGSDPNKYPSVL+EQGLL  F   DDG+ PFQ FVSEDGVENI
Sbjct: 63  CPLATTLSSKFSVLLLERGSDPNKYPSVLDEQGLLNVFAAGDDGRNPFQRFVSEDGVENI 122

Query: 124 RGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSAF 183
           RGRVLGGGSMVNAGFYSR H+EFF SAGVDWDMELVEKAYEWVEE+VVSQPIL  WQSAF
Sbjct: 123 RGRVLGGGSMVNAGFYSRGHREFFASAGVDWDMELVEKAYEWVEETVVSQPILNAWQSAF 182

Query: 184 RSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATVR 243
           RS+LLEGG+VPD+GFDLRHLVGTKTGGSI D+KGNRHGAVELLNKA+P N+KVAIEATV+
Sbjct: 183 RSSLLEGGVVPDSGFDLRHLVGTKTGGSIFDNKGNRHGAVELLNKANPTNIKVAIEATVQ 242

Query: 244 RILFSDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPKSHLSSLK 303
           RILFS LSANGV Y DSKGKLH A I +KGEI +SAGAIGSPQLLLLSGIGPKSHLSSLK
Sbjct: 243 RILFSGLSANGVLYSDSKGKLHRAIIRKKGEIIVSAGAIGSPQLLLLSGIGPKSHLSSLK 302

Query: 304 LPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASPSPFLVPP 363
           LPVVLHQP+VGQSM+DNPRFGTNII+PF V+P+S KVVGILQDNIY QSIASP P L+P 
Sbjct: 303 LPVVLHQPYVGQSMSDNPRFGTNIIIPFPVLPSSVKVVGILQDNIYIQSIASPFPILIPQ 362

Query: 364 TFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDLARC 423
            FSLLPP  TSI PTLA+F GKFSEVHS+GSL+LNSS +VKK+PIV FNYYSHPDDL RC
Sbjct: 363 IFSLLPPQATSIIPTLAMFVGKFSEVHSEGSLRLNSSTNVKKSPIVGFNYYSHPDDLGRC 422

Query: 424 VKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVATYWH 483
           VKGVRK+GD+ KT T+EKIKT+NL+GNKGF FLG+PLP NLWNDS+VEEYCKKTV TYWH
Sbjct: 423 VKGVRKMGDLLKTRTMEKIKTKNLEGNKGFEFLGVPLPENLWNDSSVEEYCKKTVTTYWH 482

Query: 484 YHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGR 531
           YHGGCLVGKVVDGNYKVI  +NLRVVDGSTFS SPGTNPMATLMMLGR
Sbjct: 483 YHGGCLVGKVVDGNYKVIGIKNLRVVDGSTFSESPGTNPMATLMMLGR 530

BLAST of CsGy2G005904 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 517.7 bits (1332), Expect = 1.1e-146
Identity = 263/547 (48.08%), Postives = 363/547 (66.36%), Query Frame = 0

Query: 7   ANILILNLIISIFQLQVHSSHAIP--NQDVSYMKFVHNATDLPRKEEFDYIIIGGGTAGC 66
           +++L   L++ +    VH S+A P  N+   +M+F+ NATD   ++ +DYII+GGGTAGC
Sbjct: 7   SSLLYTALVVLLLLGVVHRSNARPRVNRPPGFMRFISNATDFASEDYYDYIIVGGGTAGC 66

Query: 67  PLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVENIR 126
           PLA TLS  F VLLLERG  P   P+V++  G L      ++  +P Q F+SE+GV N R
Sbjct: 67  PLAATLSQSFRVLLLERGGVPYNRPNVMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNAR 126

Query: 127 GRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSAFR 186
           GRVLGG S +NAGFYSRA K+FFE++G+ WD+  V ++YEWVE ++V +P L+ WQ+A R
Sbjct: 127 GRVLGGSSAINAGFYSRADKQFFENSGLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIR 186

Query: 187 SALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATVRR 246
            ALLE G+ P NGF L H VGTK GGS  D  G RH + +LL  A   N++VA+ ATV R
Sbjct: 187 DALLEVGVHPFNGFTLEHKVGTKIGGSTFDRTGRRHSSADLLRYARSSNIRVAVYATVER 246

Query: 247 ILF--------SDLSANGVSYLDSKGKLHTAFIHEKGEIFLSAGAIGSPQLLLLSGIGPK 306
           +L         S++SA GV Y D  G+ H A I ++GE+ LSAGA+GSPQLL LSGIGP+
Sbjct: 247 VLLASSPSVSGSNVSAIGVVYRDQLGRFHHALIRDRGEVILSAGALGSPQLLFLSGIGPR 306

Query: 307 SHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIASP 366
           S+LS+  +PV L QPHVG  + DNPR G +I+ P  +  +  +VVG+ +D  + ++ ++ 
Sbjct: 307 SYLSTWGIPVALDQPHVGDFVYDNPRNGISIVPPVPMENSLIQVVGVTEDGAFLEAASNV 366

Query: 367 SPFLVP--PTFSLLPPHPTSINPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYY 426
            PF  P    F   P  P  +  T      K     S G L+L +S DV+ NP+VRFNY+
Sbjct: 367 IPFASPLHSVFIRAPASPLYVPVT--TIMEKILGPVSIGLLRL-ASTDVRINPVVRFNYF 426

Query: 427 SHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYC 486
           S P DL RCV G RK+G++ ++  ++    +   GN+ F F+G PLP +  ND  + ++C
Sbjct: 427 SDPQDLERCVNGTRKIGEILRSRAMQDFMIREWFGNRRFRFVGAPLPVDQSNDLVMADFC 486

Query: 487 KKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVG 542
           ++TV+T WHYHGG +VGKVVD + KVI   +LR+VDGSTF++SPGTNP ATLMMLGRY+G
Sbjct: 487 RRTVSTIWHYHGGAVVGKVVDSDLKVIGVNSLRLVDGSTFNISPGTNPQATLMMLGRYMG 546

BLAST of CsGy2G005904 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 412.1 bits (1058), Expect = 6.7e-115
Identity = 239/555 (43.06%), Postives = 310/555 (55.86%), Query Frame = 0

Query: 25  SSHAIPNQDVSYMKFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSD 84
           SS   PN       F+ +AT  P    +DYIIIGGGTAGCPLA TLS   SVLLLERG  
Sbjct: 24  SSDKAPN-----YSFMRDATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERGDS 83

Query: 85  PNKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHK 144
           P   P++        A     +  +P Q FVSEDGV N R RVLGGGS +NAGFY+RA  
Sbjct: 84  PYNNPNITRLSAFGAALSDLSE-SSPSQRFVSEDGVINARARVLGGGSALNAGFYTRAGT 143

Query: 145 EFFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLV 204
           ++  + G  WD  L  ++Y+WVE  V  QP +  WQ+A R  LLE GIVP+NGF   H+ 
Sbjct: 144 KYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDHIN 203

Query: 205 GTKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATVRRILFSDLS-----ANGVSYLD 264
           GTK GG+I D  GNRH A +LL  ADPK + V + ATV RILF         ANGV Y D
Sbjct: 204 GTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVYRD 263

Query: 265 SKGKLHTAFIHE--KGEIFLSAGAIGSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSM 324
             G+ H A++ E    EI LSAG +GSPQLL+LSG+GP + L +  + VV+ QPHVGQ M
Sbjct: 264 RTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQGM 323

Query: 325 TDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSI----------ASPSPFLVPPTFSL 384
            DNP     +  P  V  +  +VVGI  +  Y ++            S         +++
Sbjct: 324 YDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYYAM 383

Query: 385 LPPHPTSINPTL------------AIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYS 444
             P  T +                     K     S G L+L +  + K NPIV FNY+ 
Sbjct: 384 FSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLELKTR-NPKDNPIVTFNYFQ 443

Query: 445 HPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNLWN--------- 504
           HPDDL RCV+G++ +  V ++    + K  ++   +  + L    P NL           
Sbjct: 444 HPDDLKRCVRGIQTIERVVQSKAFSRYKYADV-SFEYLLNLTASTPVNLRPPRSGPGASL 503

Query: 505 DSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATL 542
             + EE+C+ TV T WHYHGGC+VG+VVDG+YKVI  + LRV+D ST    PGTNP AT+
Sbjct: 504 PPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTNPQATV 563

BLAST of CsGy2G005904 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 385.6 bits (989), Expect = 6.7e-107
Identity = 236/544 (43.38%), Postives = 315/544 (57.90%), Query Frame = 0

Query: 38  KFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGL 97
           +F+ +AT  P+   FDYIIIGGGTAGC LA TLS   +VL+LERG  P   P+  +    
Sbjct: 32  RFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQNATVLVLERGGSPYDDPAATD---- 91

Query: 98  LKAFDTEDDGKTP---FQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDW 157
           +  F       TP    Q F+SEDGV N R RVLGGG+++NAGFYSRA ++F   AG  W
Sbjct: 92  IGNFANTLLNITPNSWSQLFISEDGVFNSRARVLGGGTVINAGFYSRAEEDFVAEAG--W 151

Query: 158 DMELVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIID 217
           + + VE AYEWVE+ VV +P +  WQSAFR  LLE G+ P NGF   H+VGTK GG+I D
Sbjct: 152 ERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVTPYNGFTYEHIVGTKFGGTIFD 211

Query: 218 DKGNRHGAVELLNKADPKNLKVAIEATVRRILFS-----DLSANGVSYLDSKGKLHTAFI 277
             G+RH A  LL  A+P  + V + A+V +ILF+        A GV +LD+ G  + A +
Sbjct: 212 RDGHRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQRPKAYGVIFLDANGVSYKAEL 271

Query: 278 HEK----GEIFLSAGAIGSPQLLLLSGIGPKSHLSSLKL-PVVLHQPHVGQSMTDNPRFG 337
             +     E+ LSAGAI SPQLL+LSG+GP +HL++ ++ PV++ QP VGQ M DNP   
Sbjct: 272 ATQDSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRVNPVIVDQPMVGQGMGDNPMNP 331

Query: 338 TNIILPFQVVPTSGKVVGILQDNIYFQ--SIASPSPFLVPPTF---------SLLPPHPT 397
             I  P  V  +  + VGI +   Y +  S  S S  L    F         + LP    
Sbjct: 332 VFIPSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLTRSFFDGVLNLLKKTKLPTQSI 391

Query: 398 S-----------INPTLAIFFGKFSEVHSKGSLKLNSSIDVKKNPIVRFNYYSHPDDLAR 457
           S           +     +   K +   S+G L+L ++ +   NP V FNY+  P+DL +
Sbjct: 392 SKFFKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELRNT-NPDDNPSVTFNYFKDPEDLNK 451

Query: 458 CVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNL-----WNDSAVEEYCKKT 517
           CV+G+  +  V  +    K K   L   +G + L L LP NL      +   +E+YC  T
Sbjct: 452 CVEGLSTIIKVIDSKGYSKYK-YPLASARGLLNLILALPTNLRPRHITSTFDLEQYCIDT 511

Query: 518 VATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKI 542
           V T +HYHGGC VGKVVD NYKV+  + LR++DGSTF  SPGTNP AT+MMLGRY+G KI
Sbjct: 512 VMTIYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLKSPGTNPQATIMMLGRYMGQKI 567

BLAST of CsGy2G005904 vs. TAIR 10
Match: AT5G51950.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 384.8 bits (987), Expect = 1.1e-106
Identity = 236/562 (41.99%), Postives = 316/562 (56.23%), Query Frame = 0

Query: 27  HAIPNQD-VSYMKFVHNATDLPRKEEFDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDP 86
           HA  + D   Y  F+ +AT  P    FDYIIIGGGT+GC LA TLS   SVL+LERG  P
Sbjct: 20  HASCSPDKAGYYSFMKDATLAPMFARFDYIIIGGGTSGCALAATLSQNASVLVLERGGAP 79

Query: 87  NKYPSVLNEQGLLKAFDTEDDGKTPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKE 146
              P+  + +       +    K+  Q F+SEDGV N R RVLGGGS++NAGFY+RA  E
Sbjct: 80  YDNPTATDIENFATTL-SNTSPKSWSQLFISEDGVYNTRARVLGGGSVLNAGFYTRAGDE 139

Query: 147 FFESAGVDWDMELVEKAYEWVEESVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVG 206
           + +    +W  + VE AYEWVE+ V  QP +  WQ+AF+  LLE G  P NGF   H+ G
Sbjct: 140 YVKE--TEWKTDEVEAAYEWVEKKVAFQPPVLGWQTAFKDGLLEAGEFPYNGFTYDHIYG 199

Query: 207 TKTGGSIIDDKGNRHGAVELLNKADPKNLKVAIEATVRRILFSDL-----SANGVSYLDS 266
           TK GG+I D  G+RH A +LL  A+P N+ V + A+V +ILF+        A GV + D+
Sbjct: 200 TKIGGTIFDRAGHRHTAADLLEYANPGNIVVYLHASVHKILFTTKGRPRPKAYGVIFQDA 259

Query: 267 KGKLHTAFIHEK--GEIFLSAGAIGSPQLLLLSGIGPKSHLSSLKL-PVVLHQPHVGQSM 326
            G LH A + +    E+ LSAGAIGSPQLL+LSGIGP +HL++  + P+VL  P VGQ M
Sbjct: 260 NGVLHKAELEKNSMNEVILSAGAIGSPQLLMLSGIGPAAHLAAHGIKPLVLDHPMVGQGM 319

Query: 327 TDNPRFGTNIILPFQVVPTSGKVVGILQDNIYFQSIA----------------------- 386
            DNP     I  P  V  +  +VVGI +   Y +  +                       
Sbjct: 320 GDNPMNAIFIPSPTPVEVSLIQVVGITKFESYIEGASGVIFSYSWTRRFFDGVLNYLNEM 379

Query: 387 --SPSPFLVPPTFSL--LPPHPTSINPTL------AIFFGKFSEVHSKGSLKLNSSIDVK 446
             S +     PT S   +      INP L       +   K +   S+G L+L ++ +  
Sbjct: 380 QTSRTTSTTSPTLSTQSITDFFNPINPLLNATTRAGLILQKIAGPISRGHLELRNT-NPD 439

Query: 447 KNPIVRFNYYSHPDDLARCVKGVRKLGDVFKTPTLEKIKTQNLKGNKGFMFLGLPLPGNL 506
            NP VRFNYY  P+DL  CV+G+  +  V  +    K K  +   + G + L L +P NL
Sbjct: 440 DNPSVRFNYYQEPEDLQICVEGINTIIKVINSKAFSKFKYPDATIH-GLLDLMLSVPTNL 499

Query: 507 -----WNDSAVEEYCKKTVATYWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPG 542
                 +   + ++C  TV T WHYHGGC VG+VVD NY+V+  ++LRV+DGSTF  SPG
Sbjct: 500 RPRHITSMFNLRQFCIDTVMTIWHYHGGCQVGRVVDKNYRVLGIDSLRVIDGSTFLKSPG 559

BLAST of CsGy2G005904 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 383.3 bits (983), Expect = 3.3e-106
Identity = 231/541 (42.70%), Postives = 306/541 (56.56%), Query Frame = 0

Query: 52  FDYIIIGGGTAGCPLATTLSSKFSVLLLERGSDPNKYPSVLNEQGLLKAFD---TEDDGK 111
           +DYI+IGGGTAGCPLA TLS  FSVL+LERG  P    +V      L+ F     +    
Sbjct: 63  YDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANV----SFLRNFHIGLADISAS 122

Query: 112 TPFQHFVSEDGVENIRGRVLGGGSMVNAGFYSRAHKEFFESAGVDWDMELVEKAYEWVEE 171
           +  Q FVS DGV N R RVLGGGS +NAGFYSRA   F + AG  WD +LV+++Y WVE 
Sbjct: 123 SASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPWVER 182

Query: 172 SVVSQPILKDWQSAFRSALLEGGIVPDNGFDLRHLVGTKTGGSIIDDKGNRHGAVELLNK 231
            +V QP L  WQ A R +LLE G+ P NGF   H+ GTK GG+I D  G RH A ELL  
Sbjct: 183 EIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAELLAY 242

Query: 232 ADPKNLKVAIEATVRRILFSDLS----ANGVSYLDSKGKLHTAFIHEK--GEIFLSAGAI 291
           A+P+ L+V I ATV++I+F          GV + D KG  H A +  +   E+ LS+GAI
Sbjct: 243 ANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSSGAI 302

Query: 292 GSPQLLLLSGIGPKSHLSSLKLPVVLHQPHVGQSMTDNPRFGTNIILPFQ--VVPTSGKV 351
           GSPQ+L+LSGIGPK  L  LK+PVVL   HVG+ M DNP     I++P +  +  +  + 
Sbjct: 303 GSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPM--NTILVPSKAPIEQSLIQT 362

Query: 352 VGILQDNIYFQSIA--SPSPFLV----------PPTFSLLPPHPTSINPTLAIFFGKFSE 411
           VGI +  +Y ++      SP  +             FS +P        T A       +
Sbjct: 363 VGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKYQ 422

Query: 412 VH----------------SKGSLKL-NSSIDVKKNPIVRFNYYSHPDDLARCVKGVRKLG 471
           +H                S+G L L N+++D   NP V FNY+ HP DL RCV+ +R + 
Sbjct: 423 LHEAFNGSFILEKLAYPISRGHLSLVNTNVD--DNPSVTFNYFKHPVDLQRCVEAIRLVS 482

Query: 472 DVFKT-----------PTLEKIKTQNLKGNKGFMFLGLPLPGNLWNDSAVEEYCKKTVAT 531
            V  +             + K+ + ++K N          P  L +  ++ ++CK TV T
Sbjct: 483 KVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLR------PKQLNDTKSMAQFCKDTVVT 542

Query: 532 YWHYHGGCLVGKVVDGNYKVIETENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKILQQ 542
            WHYHGGCLVGKVV  N KV+  + LRV+DGSTF  SPGTNP AT+MM+GRY+G+KIL++
Sbjct: 543 IWHYHGGCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRE 587

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P527061.1e-15750.92(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1[more]
Q945K21.2e-15349.82(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1[more]
P527077.4e-15149.36(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1[more]
O827842.6e-14849.08(R)-mandelonitrile lyase 4 OS=Prunus serotina OX=23207 GN=MDL4 PE=2 SV=1[more]
O242436.5e-14748.53(R)-mandelonitrile lyase 1 OS=Prunus dulcis OX=3755 GN=MDL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
XP_031736583.10.0100.00(R)-mandelonitrile lyase 1 [Cucumis sativus] >KAE8651658.1 hypothetical protein ... [more]
XP_011650118.20.094.29(R)-mandelonitrile lyase 1-like [Cucumis sativus] >KGN61134.2 hypothetical prote... [more]
XP_008451990.10.089.50PREDICTED: (R)-mandelonitrile lyase 1-like isoform X1 [Cucumis melo][more]
XP_008452005.20.089.43PREDICTED: (R)-mandelonitrile lyase 1-like isoform X2 [Cucumis melo][more]
XP_011650116.20.084.26(R)-mandelonitrile lyase 1 [Cucumis sativus][more]
Match NameE-valueIdentityDescription
A0A0A0LZU80.099.62Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G627390 PE=3 SV=1[more]
A0A1S3BSR30.089.50(R)-mandelonitrile lyase 1-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC1034931... [more]
A0A1S3BSS80.089.43(R)-mandelonitrile lyase 1-like isoform X2 OS=Cucumis melo OX=3656 GN=LOC1034931... [more]
A0A1S3BTY80.089.55(R)-mandelonitrile lyase 1-like isoform X3 OS=Cucumis melo OX=3656 GN=LOC1034931... [more]
A0A0A0LK760.084.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G055000 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G73050.11.1e-14648.08Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G12570.16.7e-11543.06Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT3G56060.16.7e-10743.38Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT5G51950.11.1e-10641.99Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
AT1G72970.13.3e-10642.70Glucose-methanol-choline (GMC) oxidoreductase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucumber (Gy14) v2.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.410.40coord: 172..480
e-value: 2.0E-166
score: 556.7
NoneNo IPR availablePANTHERPTHR45968:SF23(R)-MANDELONITRILE LYASE 4-RELATEDcoord: 2..542
NoneNo IPR availablePANTHERPTHR45968OSJNBA0019K04.7 PROTEINcoord: 2..542
NoneNo IPR availableSUPERFAMILY54373FAD-linked reductases, C-terminal domaincoord: 390..487
IPR012132Glucose-methanol-choline oxidoreductasePIRSFPIRSF000137Alcohol_oxidasecoord: 354..543
e-value: 2.9E-28
score: 96.9
coord: 35..347
e-value: 8.4E-58
score: 194.5
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPFAMPF00732GMC_oxred_Ncoord: 52..321
e-value: 1.1E-31
score: 110.3
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00623GMC_OXRED_1coord: 125..148
IPR000172Glucose-methanol-choline oxidoreductase, N-terminalPROSITEPS00624GMC_OXRED_2coord: 280..294
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 52..530
e-value: 2.0E-166
score: 556.7
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 33..541
IPR007867Glucose-methanol-choline oxidoreductase, C-terminalPFAMPF05199GMC_oxred_Ccoord: 390..531
e-value: 8.6E-28
score: 97.6

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CsGy2G005904.1CsGy2G005904.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016829 lyase activity
molecular_function GO:0016614 oxidoreductase activity, acting on CH-OH group of donors