Cp4.1LG19g07110 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG19g07110
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Descriptionheat stress transcription factor B-2a-like
LocationCp4.1LG19: 7088390 .. 7090003 (-)
RNA-Seq ExpressionCp4.1LG19g07110
SyntenyCp4.1LG19g07110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTCAGAGTTCGCGGCCCTTCTTTTGAAATTACTCGTCAATTTTCAACATTTCATCATGGAAGACAATTATATGATTCTTTTCCCCGCAAAAACCATTCCTTAATTTCATTTTCTCTAACTATGTAATACTCTTATTTATAGATGCAGAAAATTAAGAAAATTTTCTATATAACTTATTATAAAAATATGATTTTGTGGTAAGTAGGAACATATTCATGTGGAGGAAATTGTTAAAATATACAAGCGATCAATTTGAATAGTCGAGTTCACATTTGGAGTGTTTTCAATCCAATTGATTCTATATTATTCTAGATCATGTTTTTCTATGATTACTAGAAGAACAATGGCTTTGATTTTACAACTATTTTGTTAACGTAATAGACTTCAATGGGTCCAAACAGCCTTGAAGAAAAAGCAGAATTTGGAAAAGGGAAATAAAGGATAAAGGAAACCTCTACCCTTTATAGAACGTAACCTGGTAGCTTCCTAGCAAATGGCTGCAGGTGAAGGGCTTATAAGTTGAGAAGCTGATCAACTTTGAACAGATTTTGGGTGTGAATTTGATTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTGTCAAAGACTTATGATCTGTTAGAAGAGGCTGGAAGCTTCCATCTTAAAGCAGCAGATCAAGAGGAGGAAGAAGAAGAAGAAGAAGAAAATAGGATTGTGTCCTGGAATGCAGAGGGAAATGGGTTCATAGTTTGGTCTCCTGCGGAATTCTCTGAGGTTTTGTTGCCTAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTACGTATGTATTCTCTCTCTCTCTCTGGCTCTCTCTTTTTGAAAATGCCCCCATCACTTTTTATTCTCTGAAACATCTTGAAGAAGAATTATTCATTATAATGAGGATCCAATTGACCACTTTCCTGATTCGAATGCTTGAATTCTGCCTTAGAAATTGGATCTAATAGGTAATATGTTATCTATTTTATGTGTTCCTTATGCTCCCTCTCACATGGGTTTGGCCATGAATACCCATTGATATTAATGGAAAAGGAAATGAGATTTTTGATAGGTTATGATATATTTGAAACAGGGGTTCAAGAAGACGTCGCAAAAGAGGTGGGAATTCAAGCATGAGAAGTTTCAGAGGGGCAACAAGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCTAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGAGCTGAAACAATGGCGGTTGGTCAGAAGAATGGCGACCATCTGCAACTAATGGAAGAGAACAAGAATCTAAGAAGACGGAAGCTGGAATTGCAAGCTCGAATAGCCCGATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGATCAGTGCATTAAGAAGTAGCCTACAGAGTTTTGCTAATTAGAAAATGGGGTTGGTTGTGAAAATTTGAGAAAGAAAGTAATACTTTGGGTTATGCATTTGCTCCAAATTGAGTTTCCATTGTCCACTCTAATTAGTTCTAGAGAAGTTGCCATCTGTAAAAGTTGATATGAACAATGAACAATTAACAAAACATTTTATAGTTTAT

mRNA sequence

GTCAGAGTTCGCGGCCCTTCTTTTGAAATTACTCGTCAATTTTCAACATTTCATCATGGAAGACAATTATATGATTCTTTTCCCCGCAAAAACCATTCCTTAATTTCATTTTCTCTAACTATGTAATACTCTTATTTATAGATGCAGAAAATTAAGAAAATTTTCTATATAACTTATTATAAAAATATGATTTTGTGGTAAGTAGGAACATATTCATGTGGAGGAAATTGTTAAAATATACAAGCGATCAATTTGAATAGTCGAGTTCACATTTGGAGTGTTTTCAATCCAATTGATTCTATATTATTCTAGATCATGTTTTTCTATGATTACTAGAAGAACAATGGCTTTGATTTTACAACTATTTTGTTAACGTAATAGACTTCAATGGGTCCAAACAGCCTTGAAGAAAAAGCAGAATTTGGAAAAGGGAAATAAAGGATAAAGGAAACCTCTACCCTTTATAGAACGTAACCTGGTAGCTTCCTAGCAAATGGCTGCAGGTGAAGGGCTTATAAGTTGAGAAGCTGATCAACTTTGAACAGATTTTGGGTGTGAATTTGATTATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTGTCAAAGACTTATGATCTGTTAGAAGAGGCTGGAAGCTTCCATCTTAAAGCAGCAGATCAAGAGGAGGAAGAAGAAGAAGAAGAAGAAAATAGGATTGTGTCCTGGAATGCAGAGGGAAATGGGTTCATAGTTTGGTCTCCTGCGGAATTCTCTGAGGTTTTGTTGCCTAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTACGGGTTCAAGAAGACGTCGCAAAAGAGGTGGGAATTCAAGCATGAGAAGTTTCAGAGGGGCAACAAGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCTAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGAGCTGAAACAATGGCGGTTGGTCAGAAGAATGGCGACCATCTGCAACTAATGGAAGAGAACAAGAATCTAAGAAGACGGAAGCTGGAATTGCAAGCTCGAATAGCCCGATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGATCAGTGCATTAAGAAGTAGCCTACAGAGTTTTGCTAATTAGAAAATGGGGTTGGTTGTGAAAATTTGAGAAAGAAAGTAATACTTTGGGTTATGCATTTGCTCCAAATTGAGTTTCCATTGTCCACTCTAATTAGTTCTAGAGAAGTTGCCATCTGTAAAAGTTGATATGAACAATGAACAATTAACAAAACATTTTATAGTTTAT

Coding sequence (CDS)

ATGCAGTCTTCAAAAACCAGAAACCCTGCTCCTTTTCTGTCAAAGACTTATGATCTGTTAGAAGAGGCTGGAAGCTTCCATCTTAAAGCAGCAGATCAAGAGGAGGAAGAAGAAGAAGAAGAAGAAAATAGGATTGTGTCCTGGAATGCAGAGGGAAATGGGTTCATAGTTTGGTCTCCTGCGGAATTCTCTGAGGTTTTGTTGCCTAAGTACTTCAAGCATAATAATTTCTCTAGCTTCATTCGCCAGCTTAATACTTACGGGTTCAAGAAGACGTCGCAAAAGAGGTGGGAATTCAAGCATGAGAAGTTTCAGAGGGGCAACAAGCAGATGCTGGGGGAGATTGTACGTAAGAAATGTGAGCCTAGTGTGTTTCCAGCCTTTCTAAGATCTTCTCATGAAGGAGCTGAAACAATGGCGGTTGGTCAGAAGAATGGCGACCATCTGCAACTAATGGAAGAGAACAAGAATCTAAGAAGACGGAAGCTGGAATTGCAAGCTCGAATAGCCCGATTCAAAGCACTTCATATTAGATTACTGGACTGTCTAGATCAGTGCATTAAGAAGTAG

Protein sequence

MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCEPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLLDCLDQCIKK
Homology
BLAST of Cp4.1LG19g07110 vs. ExPASy Swiss-Prot
Match: Q9SCW4 (Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A PE=2 SV=1)

HSP 1 Score: 126.3 bits (316), Expect = 3.6e-28
Identity = 71/202 (35.15%), Postives = 109/202 (53.96%), Query Frame = 0

Query: 3   SSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAE 62
           SS+   P PFL+KT++L+E++                   + ++SWN +G+ FIVW+P +
Sbjct: 15  SSQRSIPTPFLTKTFNLVEDSSI-----------------DDVISWNEDGSSFIVWNPTD 74

Query: 63  FSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCEP 122
           F++ LLPK+FKHNNFSSF+RQLNTYGFKK    RWEF ++ F+RG K++L EI R+K   
Sbjct: 75  FAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITT 134

Query: 123 SVFPAFLRSSHEGAETMAVGQKN-----------------------------GDHLQLME 176
           +       SS +  +TM V   N                             G  ++L+E
Sbjct: 135 THQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLE 194

BLAST of Cp4.1LG19g07110 vs. ExPASy Swiss-Prot
Match: O81821 (Heat stress transcription factor A-1b OS=Arabidopsis thaliana OX=3702 GN=HSFA1B PE=1 SV=2)

HSP 1 Score: 120.9 bits (302), Expect = 1.5e-26
Identity = 72/166 (43.37%), Postives = 98/166 (59.04%), Query Frame = 0

Query: 11  PFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLLPK 70
           PFLSKTYD++++  +                 N +VSW++  N F+VWS  EFS+VLLPK
Sbjct: 27  PFLSKTYDMVDDPLT-----------------NEVVSWSSGNNSFVVWSAPEFSKVLLPK 86

Query: 71  YFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKK---CEPSVFPA 130
           YFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG KQ+L  IVR+K    + +    
Sbjct: 87  YFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQT 146

Query: 131 FLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFK 174
            ++SS  GA  + VG+       + EE + L+R K  L   + R +
Sbjct: 147 QVQSSSVGA-CVEVGK-----FGIEEEVERLKRDKNVLMQELVRLR 169

BLAST of Cp4.1LG19g07110 vs. ExPASy Swiss-Prot
Match: P41151 (Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 9.8e-26
Identity = 73/174 (41.95%), Postives = 96/174 (55.17%), Query Frame = 0

Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFLSKTYD++E+  +                 + IVSW+   N FIVW P EFS  LL
Sbjct: 50  PPPFLSKTYDMVEDPAT-----------------DAIVSWSPTNNSFIVWDPPEFSRDLL 109

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCEPSVFPAF 128
           PKYFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG K +L +I R+K   SV    
Sbjct: 110 PKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRK---SVQGHG 169

Query: 129 LRSSHEGAETMAVGQKNGDHLQ---------LMEENKNLRRRKLELQARIARFK 174
             SS+  ++ ++ GQ +   L          L EE + L+R K  L   + + +
Sbjct: 170 SSSSNPQSQQLSQGQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLR 203

BLAST of Cp4.1LG19g07110 vs. ExPASy Swiss-Prot
Match: Q9LQM7 (Heat stress transcription factor A-1d OS=Arabidopsis thaliana OX=3702 GN=HSFA1D PE=1 SV=2)

HSP 1 Score: 118.2 bits (295), Expect = 9.8e-26
Identity = 69/166 (41.57%), Postives = 87/166 (52.41%), Query Frame = 0

Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFLSKTYD++                 ++   + IVSW+A  N FIVW P EF+  LL
Sbjct: 35  PPPFLSKTYDMV-----------------DDHNTDSIVSWSANNNSFIVWKPPEFARDLL 94

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCEPSVFPAF 128
           PK FKHNNFSSF+RQLNTYGF+K    RWEF +E F RG K +L  I R+K         
Sbjct: 95  PKNFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQGQGH 154

Query: 129 LRSSHEGAETMAVGQ-KNGDHLQLMEENKNLRRRKLELQARIARFK 174
            RS H   +  +V          L EE + L+R K  L   + R +
Sbjct: 155 QRSQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLR 183

BLAST of Cp4.1LG19g07110 vs. ExPASy Swiss-Prot
Match: Q6Z9C8 (Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 GN=HSFB2B PE=2 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 1.7e-25
Identity = 62/133 (46.62%), Postives = 84/133 (63.16%), Query Frame = 0

Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFL+KTY L+++       A D            ++SWN +G+ F+VW PAEF+  LL
Sbjct: 46  PTPFLTKTYQLVDD------PAVDD-----------VISWNDDGSTFVVWRPAEFARDLL 105

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCEPSVFPAF 128
           PKYFKHNNFSSF+RQLNTYGF+K    RWEF ++ F+RG +++L EI R+K  P   PA 
Sbjct: 106 PKYFKHNNFSSFVRQLNTYGFRKIVPDRWEFANDCFRRGERRLLCEIHRRKVTPPA-PAA 160

Query: 129 LRSSHEGAETMAV 142
             ++   A  MA+
Sbjct: 166 TTAAVAAAIPMAL 160

BLAST of Cp4.1LG19g07110 vs. NCBI nr
Match: XP_023518612.1 (heat stress transcription factor B-2a-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 375 bits (964), Expect = 3.20e-131
Identity = 189/189 (100.00%), Postives = 189/189 (100.00%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP 60
           MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP 60

Query: 61  AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKC 120
           AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKC
Sbjct: 61  AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKC 120

Query: 121 EPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLL 180
           EPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLL
Sbjct: 121 EPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLL 180

Query: 181 DCLDQCIKK 189
           DCLDQCIKK
Sbjct: 181 DCLDQCIKK 189

BLAST of Cp4.1LG19g07110 vs. NCBI nr
Match: XP_023003186.1 (heat stress transcription factor B-2a-like [Cucurbita maxima])

HSP 1 Score: 372 bits (954), Expect = 1.07e-129
Identity = 186/189 (98.41%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP 60
           MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP 60

Query: 61  AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKC 120
           AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEI+RKKC
Sbjct: 61  AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKC 120

Query: 121 EPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLL 180
           EPSVFPAFLRSSHEG+ETMAVGQKNGDHLQLMEENKNLRRRKLELQARI RFKALHIRLL
Sbjct: 121 EPSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIRLL 180

Query: 181 DCLDQCIKK 189
           DCLDQCIKK
Sbjct: 181 DCLDQCIKK 189

BLAST of Cp4.1LG19g07110 vs. NCBI nr
Match: XP_022926806.1 (heat stress transcription factor B-2a-like [Cucurbita moschata])

HSP 1 Score: 368 bits (944), Expect = 3.86e-128
Identity = 187/191 (97.91%), Postives = 188/191 (98.43%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE--NRIVSWNAEGNGFIVW 60
           MQSSKTRNPAPFLSKTYDLLEEAGSFH KAADQEEEEEEEEE  NRIVSWNAEGNGFIVW
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEEEEEEEEEEENRIVSWNAEGNGFIVW 60

Query: 61  SPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRK 120
           SPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRK
Sbjct: 61  SPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRK 120

Query: 121 KCEPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIR 180
           KCEPSVFPAFLRSSHEGAETMAVGQ+NGDHLQLMEENKNLRRRKLELQARIARFKALHIR
Sbjct: 121 KCEPSVFPAFLRSSHEGAETMAVGQRNGDHLQLMEENKNLRRRKLELQARIARFKALHIR 180

Query: 181 LLDCLDQCIKK 189
           LLDCLDQCIKK
Sbjct: 181 LLDCLDQCIKK 191

BLAST of Cp4.1LG19g07110 vs. NCBI nr
Match: KAG7026576.1 (Heat stress transcription factor B-2a, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 366 bits (939), Expect = 2.01e-127
Identity = 184/189 (97.35%), Postives = 187/189 (98.94%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP 60
           MQSSKTRNPAPFLSKTYDLLEEAGSFH KAADQEEEEEEEE NR+VSWNAEGNGF+VWSP
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEEEEEEEE-NRVVSWNAEGNGFVVWSP 60

Query: 61  AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKC 120
           AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKC
Sbjct: 61  AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKC 120

Query: 121 EPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLL 180
           EPSVFPAFLRSSHEGAETMAVGQ+NGDHLQLMEENKNLRRRKLELQARIARFKALHIRLL
Sbjct: 121 EPSVFPAFLRSSHEGAETMAVGQRNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLL 180

Query: 181 DCLDQCIKK 189
           DCLDQCIKK
Sbjct: 181 DCLDQCIKK 188

BLAST of Cp4.1LG19g07110 vs. NCBI nr
Match: KAG6594605.1 (E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 364 bits (935), Expect = 5.65e-123
Identity = 185/188 (98.40%), Postives = 186/188 (98.94%), Query Frame = 0

Query: 3   SSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE-NRIVSWNAEGNGFIVWSPA 62
           SSKTRNPAPFLSKTYDLLEEAGSFH KAADQEEEEEEEEE NRIVSWNAEGNGFIVWSPA
Sbjct: 260 SSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEEEEEEEEEENRIVSWNAEGNGFIVWSPA 319

Query: 63  EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCE 122
           EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCE
Sbjct: 320 EFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCE 379

Query: 123 PSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLLD 182
           PSVFPAFLRSSHEGAETMAVGQ+NGDHLQLMEENKNLRRRKLELQARIARFKALHIRLLD
Sbjct: 380 PSVFPAFLRSSHEGAETMAVGQRNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLLD 439

Query: 183 CLDQCIKK 189
           CLDQCIKK
Sbjct: 440 CLDQCIKK 447

BLAST of Cp4.1LG19g07110 vs. ExPASy TrEMBL
Match: A0A6J1KNE4 (heat stress transcription factor B-2a-like OS=Cucurbita maxima OX=3661 GN=LOC111496875 PE=3 SV=1)

HSP 1 Score: 372 bits (954), Expect = 5.19e-130
Identity = 186/189 (98.41%), Postives = 188/189 (99.47%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP 60
           MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSP 60

Query: 61  AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKC 120
           AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEI+RKKC
Sbjct: 61  AEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEILRKKC 120

Query: 121 EPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIRLL 180
           EPSVFPAFLRSSHEG+ETMAVGQKNGDHLQLMEENKNLRRRKLELQARI RFKALHIRLL
Sbjct: 121 EPSVFPAFLRSSHEGSETMAVGQKNGDHLQLMEENKNLRRRKLELQARIVRFKALHIRLL 180

Query: 181 DCLDQCIKK 189
           DCLDQCIKK
Sbjct: 181 DCLDQCIKK 189

BLAST of Cp4.1LG19g07110 vs. ExPASy TrEMBL
Match: A0A6J1EFX0 (heat stress transcription factor B-2a-like OS=Cucurbita moschata OX=3662 GN=LOC111433815 PE=3 SV=1)

HSP 1 Score: 368 bits (944), Expect = 1.87e-128
Identity = 187/191 (97.91%), Postives = 188/191 (98.43%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE--NRIVSWNAEGNGFIVW 60
           MQSSKTRNPAPFLSKTYDLLEEAGSFH KAADQEEEEEEEEE  NRIVSWNAEGNGFIVW
Sbjct: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHFKAADQEEEEEEEEEEENRIVSWNAEGNGFIVW 60

Query: 61  SPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRK 120
           SPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRK
Sbjct: 61  SPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRK 120

Query: 121 KCEPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKALHIR 180
           KCEPSVFPAFLRSSHEGAETMAVGQ+NGDHLQLMEENKNLRRRKLELQARIARFKALHIR
Sbjct: 121 KCEPSVFPAFLRSSHEGAETMAVGQRNGDHLQLMEENKNLRRRKLELQARIARFKALHIR 180

Query: 181 LLDCLDQCIKK 189
           LLDCLDQCIKK
Sbjct: 181 LLDCLDQCIKK 191

BLAST of Cp4.1LG19g07110 vs. ExPASy TrEMBL
Match: A0A5D3CQ35 (Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G007440 PE=3 SV=1)

HSP 1 Score: 326 bits (836), Expect = 6.04e-112
Identity = 165/194 (85.05%), Postives = 177/194 (91.24%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE-----NRIVSWNAEGNGF 60
           MQSS+TRNPAPFLSKTYDLLEEAGSFH K ADQE+E+EEEEE     NRIVSWNAEG+GF
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEEEEEEENGGNRIVSWNAEGSGF 60

Query: 61  IVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEI 120
           IVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG + ML EI
Sbjct: 61  IVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHMLVEI 120

Query: 121 VRKKCEPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFKAL 180
           VRKKCEPSVFPAFLRSSHEG  TMAV Q+NGDHL LMEEN NLRR+KLELQA+IA+FKAL
Sbjct: 121 VRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQFKAL 180

Query: 181 HIRLLDCLDQCIKK 189
           HIRLLDCL QCI+K
Sbjct: 181 HIRLLDCLGQCIEK 194

BLAST of Cp4.1LG19g07110 vs. ExPASy TrEMBL
Match: A0A5A7UBZ5 (Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold120G003020 PE=3 SV=1)

HSP 1 Score: 323 bits (829), Expect = 8.10e-111
Identity = 164/198 (82.83%), Postives = 177/198 (89.39%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE---------NRIVSWNAE 60
           MQSS+TRNPAPFLSKTYDLLEEAGSFH K ADQE+E+E+EEE         NRIVSWNAE
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGNRIVSWNAE 60

Query: 61  GNGFIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQM 120
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG + M
Sbjct: 61  GSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHM 120

Query: 121 LGEIVRKKCEPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIAR 180
           L EIVRKKCEPSVFPAFLRSSHEG  TMAV Q+NGDHL LMEEN NLRR+KLELQA+IA+
Sbjct: 121 LVEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQ 180

Query: 181 FKALHIRLLDCLDQCIKK 189
           FKALHIRLLDCL QCI+K
Sbjct: 181 FKALHIRLLDCLGQCIEK 198

BLAST of Cp4.1LG19g07110 vs. ExPASy TrEMBL
Match: A0A1S3AZS2 (heat stress transcription factor B-2a-like OS=Cucumis melo OX=3656 GN=LOC103484524 PE=3 SV=1)

HSP 1 Score: 323 bits (829), Expect = 8.10e-111
Identity = 164/198 (82.83%), Postives = 177/198 (89.39%), Query Frame = 0

Query: 1   MQSSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEE---------NRIVSWNAE 60
           MQSS+TRNPAPFLSKTYDLLEEAGSFH K ADQE+E+E+EEE         NRIVSWNAE
Sbjct: 1   MQSSRTRNPAPFLSKTYDLLEEAGSFHFKEADQEDEDEDEEEEEEEEENGGNRIVSWNAE 60

Query: 61  GNGFIVWSPAEFSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQM 120
           G+GFIVWSPA+FSE+LLPKYFKHNNFSSFIRQLNTYGFKKTS KRWEFKHEKFQRG + M
Sbjct: 61  GSGFIVWSPADFSELLLPKYFKHNNFSSFIRQLNTYGFKKTSSKRWEFKHEKFQRGKRHM 120

Query: 121 LGEIVRKKCEPSVFPAFLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIAR 180
           L EIVRKKCEPSVFPAFLRSSHEG  TMAV Q+NGDHL LMEEN NLRR+KLELQA+IA+
Sbjct: 121 LVEIVRKKCEPSVFPAFLRSSHEGGATMAVNQENGDHLLLMEENNNLRRQKLELQAQIAQ 180

Query: 181 FKALHIRLLDCLDQCIKK 189
           FKALHIRLLDCL QCI+K
Sbjct: 181 FKALHIRLLDCLGQCIEK 198

BLAST of Cp4.1LG19g07110 vs. TAIR 10
Match: AT5G62020.1 (heat shock transcription factor B2A )

HSP 1 Score: 126.3 bits (316), Expect = 2.6e-29
Identity = 71/202 (35.15%), Postives = 109/202 (53.96%), Query Frame = 0

Query: 3   SSKTRNPAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAE 62
           SS+   P PFL+KT++L+E++                   + ++SWN +G+ FIVW+P +
Sbjct: 15  SSQRSIPTPFLTKTFNLVEDSSI-----------------DDVISWNEDGSSFIVWNPTD 74

Query: 63  FSEVLLPKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCEP 122
           F++ LLPK+FKHNNFSSF+RQLNTYGFKK    RWEF ++ F+RG K++L EI R+K   
Sbjct: 75  FAKDLLPKHFKHNNFSSFVRQLNTYGFKKVVPDRWEFSNDFFKRGEKRLLREIQRRKITT 134

Query: 123 SVFPAFLRSSHEGAETMAVGQKN-----------------------------GDHLQLME 176
           +       SS +  +TM V   N                             G  ++L+E
Sbjct: 135 THQTVVAPSSEQRNQTMVVSPSNSGEDNNNNQVMSSSPSSWYCHQTKTTGNGGLSVELLE 194

BLAST of Cp4.1LG19g07110 vs. TAIR 10
Match: AT5G16820.1 (heat shock factor 3 )

HSP 1 Score: 120.9 bits (302), Expect = 1.1e-27
Identity = 72/166 (43.37%), Postives = 98/166 (59.04%), Query Frame = 0

Query: 11  PFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLLPK 70
           PFLSKTYD++++  +                 N +VSW++  N F+VWS  EFS+VLLPK
Sbjct: 27  PFLSKTYDMVDDPLT-----------------NEVVSWSSGNNSFVVWSAPEFSKVLLPK 86

Query: 71  YFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKK---CEPSVFPA 130
           YFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG KQ+L  IVR+K    + +    
Sbjct: 87  YFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQT 146

Query: 131 FLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFK 174
            ++SS  GA  + VG+       + EE + L+R K  L   + R +
Sbjct: 147 QVQSSSVGA-CVEVGK-----FGIEEEVERLKRDKNVLMQELVRLR 169

BLAST of Cp4.1LG19g07110 vs. TAIR 10
Match: AT5G16820.2 (heat shock factor 3 )

HSP 1 Score: 120.9 bits (302), Expect = 1.1e-27
Identity = 72/166 (43.37%), Postives = 98/166 (59.04%), Query Frame = 0

Query: 11  PFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLLPK 70
           PFLSKTYD++++  +                 N +VSW++  N F+VWS  EFS+VLLPK
Sbjct: 27  PFLSKTYDMVDDPLT-----------------NEVVSWSSGNNSFVVWSAPEFSKVLLPK 86

Query: 71  YFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKK---CEPSVFPA 130
           YFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG KQ+L  IVR+K    + +    
Sbjct: 87  YFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGRKQLLKSIVRRKPSHVQQNQQQT 146

Query: 131 FLRSSHEGAETMAVGQKNGDHLQLMEENKNLRRRKLELQARIARFK 174
            ++SS  GA  + VG+       + EE + L+R K  L   + R +
Sbjct: 147 QVQSSSVGA-CVEVGK-----FGIEEEVERLKRDKNVLMQELVRLR 169

BLAST of Cp4.1LG19g07110 vs. TAIR 10
Match: AT1G32330.1 (heat shock transcription factor A1D )

HSP 1 Score: 118.2 bits (295), Expect = 7.0e-27
Identity = 69/166 (41.57%), Postives = 87/166 (52.41%), Query Frame = 0

Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFLSKTYD++                 ++   + IVSW+A  N FIVW P EF+  LL
Sbjct: 35  PPPFLSKTYDMV-----------------DDHNTDSIVSWSANNNSFIVWKPPEFARDLL 94

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCEPSVFPAF 128
           PK FKHNNFSSF+RQLNTYGF+K    RWEF +E F RG K +L  I R+K         
Sbjct: 95  PKNFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLQSITRRKPAHGQGQGH 154

Query: 129 LRSSHEGAETMAVGQ-KNGDHLQLMEENKNLRRRKLELQARIARFK 174
            RS H   +  +V          L EE + L+R K  L   + R +
Sbjct: 155 QRSQHSNGQNSSVSACVEVGKFGLEEEVERLKRDKNVLMQELVRLR 183

BLAST of Cp4.1LG19g07110 vs. TAIR 10
Match: AT4G17750.1 (heat shock factor 1 )

HSP 1 Score: 118.2 bits (295), Expect = 7.0e-27
Identity = 73/174 (41.95%), Postives = 96/174 (55.17%), Query Frame = 0

Query: 9   PAPFLSKTYDLLEEAGSFHLKAADQEEEEEEEEENRIVSWNAEGNGFIVWSPAEFSEVLL 68
           P PFLSKTYD++E+  +                 + IVSW+   N FIVW P EFS  LL
Sbjct: 50  PPPFLSKTYDMVEDPAT-----------------DAIVSWSPTNNSFIVWDPPEFSRDLL 109

Query: 69  PKYFKHNNFSSFIRQLNTYGFKKTSQKRWEFKHEKFQRGNKQMLGEIVRKKCEPSVFPAF 128
           PKYFKHNNFSSF+RQLNTYGF+K    RWEF +E F RG K +L +I R+K   SV    
Sbjct: 110 PKYFKHNNFSSFVRQLNTYGFRKVDPDRWEFANEGFLRGQKHLLKKISRRK---SVQGHG 169

Query: 129 LRSSHEGAETMAVGQKNGDHLQ---------LMEENKNLRRRKLELQARIARFK 174
             SS+  ++ ++ GQ +   L          L EE + L+R K  L   + + +
Sbjct: 170 SSSSNPQSQQLSQGQGSMAALSSCVEVGKFGLEEEVEQLKRDKNVLMQELVKLR 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SCW43.6e-2835.15Heat stress transcription factor B-2a OS=Arabidopsis thaliana OX=3702 GN=HSFB2A ... [more]
O818211.5e-2643.37Heat stress transcription factor A-1b OS=Arabidopsis thaliana OX=3702 GN=HSFA1B ... [more]
P411519.8e-2641.95Heat stress transcription factor A-1a OS=Arabidopsis thaliana OX=3702 GN=HSFA1A ... [more]
Q9LQM79.8e-2641.57Heat stress transcription factor A-1d OS=Arabidopsis thaliana OX=3702 GN=HSFA1D ... [more]
Q6Z9C81.7e-2546.62Heat stress transcription factor B-2b OS=Oryza sativa subsp. japonica OX=39947 G... [more]
Match NameE-valueIdentityDescription
XP_023518612.13.20e-131100.00heat stress transcription factor B-2a-like isoform X1 [Cucurbita pepo subsp. pep... [more]
XP_023003186.11.07e-12998.41heat stress transcription factor B-2a-like [Cucurbita maxima][more]
XP_022926806.13.86e-12897.91heat stress transcription factor B-2a-like [Cucurbita moschata][more]
KAG7026576.12.01e-12797.35Heat stress transcription factor B-2a, partial [Cucurbita argyrosperma subsp. ar... [more]
KAG6594605.15.65e-12398.40E3 ubiquitin-protein ligase RMA1H1, partial [Cucurbita argyrosperma subsp. soror... [more]
Match NameE-valueIdentityDescription
A0A6J1KNE45.19e-13098.41heat stress transcription factor B-2a-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1EFX01.87e-12897.91heat stress transcription factor B-2a-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A5D3CQ356.04e-11285.05Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A5A7UBZ58.10e-11182.83Heat stress transcription factor B-2a-like OS=Cucumis melo var. makuwa OX=119469... [more]
A0A1S3AZS28.10e-11182.83heat stress transcription factor B-2a-like OS=Cucumis melo OX=3656 GN=LOC1034845... [more]
Match NameE-valueIdentityDescription
AT5G62020.12.6e-2935.15heat shock transcription factor B2A [more]
AT5G16820.11.1e-2743.37heat shock factor 3 [more]
AT5G16820.21.1e-2743.37heat shock factor 3 [more]
AT1G32330.17.0e-2741.57heat shock transcription factor A1D [more]
AT4G17750.17.0e-2741.95heat shock factor 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 148..175
NoneNo IPR availableCOILSCoilCoilcoord: 31..51
NoneNo IPR availablePANTHERPTHR10015:SF308HEAT STRESS TRANSCRIPTION FACTOR B-2A-LIKEcoord: 3..187
IPR000232Heat shock factor (HSF)-type, DNA-bindingPRINTSPR00056HSFDOMAINcoord: 67..79
score: 81.98
coord: 80..92
score: 74.51
coord: 29..52
score: 17.86
IPR000232Heat shock factor (HSF)-type, DNA-bindingSMARTSM00415hsfneu3coord: 8..118
e-value: 1.3E-38
score: 144.2
IPR000232Heat shock factor (HSF)-type, DNA-bindingPFAMPF00447HSF_DNA-bindcoord: 12..118
e-value: 1.8E-29
score: 102.2
IPR036388Winged helix-like DNA-binding domain superfamilyGENE3D1.10.10.10coord: 4..119
e-value: 1.1E-31
score: 111.3
IPR027725Heat shock transcription factor familyPANTHERPTHR10015HEAT SHOCK TRANSCRIPTION FACTORcoord: 3..187
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 39..118

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG19g07110.1Cp4.1LG19g07110.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006357 regulation of transcription by RNA polymerase II
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0000978 RNA polymerase II cis-regulatory region sequence-specific DNA binding
molecular_function GO:0043565 sequence-specific DNA binding