Cp4.1LG17g00130 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG17g00130
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectin lyase-like superfamily protein
Location	Cp4.1LG17: 486252 .. 488666 (+)
RNA-Seq Expression	Cp4.1LG17g00130
Synteny	Cp4.1LG17g00130

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ACTAATATATATATATATGTGCTACACAACAACATAGGTTCACGAGACTAATGAACTCTGATGTACCTTTTTCTCTTTCTTTTCTCTCCCTTCCACTAACCAAAACCAACATGGACCATTGGCTTTACTTCCCATTCTGTTTTAACCCATCTTCTGCTATTTCACCTTTCTTCACACCATGCATTTCTACATAAATATTTCTTTGTCCTCTAAACCCCTCCACTTTTTTACTTTTTACTATAATATCCTGCTCATCTCTCTCTCTCTCAACTCGACACCTGTTCTCTTTCTACACGTTACTTCTTTCCTTTCTCTTATAAAGTATTAACAAATGTAGATACATATGCATATATAGCTGCTTCTGTCCACCCAGCTTCCCCACTTCATTCTAACCTTTGTTCTTTACAACTCAAGTCTCTTTCTCTCCATTCCTTTCCCATTCTTTCCTCCTGCCATCCCTCTCCATTTTTGCCACTTGTTGCTCACTGCTAACTTTCTGGCCTTGCATTCTTTTCAAATTCACCTGGCTTCCATGGAGTTCAATCGAGTTCCTGCTGTAATGCTTTGGTTCACCGCCATTTTGGTGGCGGACAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGCAAGACTCGGTTCCTTCAACTCCAGCTCACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGACGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTCAGCCACATTTTTTTAGAATTTCAATCTCTTGTTGTTCGTGTTGAAGTTGGAACACTTATAAGTAAACCTGTTAATGCAACAAATCAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTTGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGTACCTACATTGTCCTCTTAATAGCTCTTGAATTTCTCTGTTTATTTCAGTTTCAACTTCCTATCCAAGGCTTAATTGTCCTTTTTTATTTTAGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGGTAAGAAGGAAACCCACCTTTTTGCCCACCAATTCAACCAACTTATATGAATAAAATATGCTGACTGGTTCTGATATCTGGGGTTTTCAGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGGCTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGACAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCATATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGGTATGAAACTGAAATAATGGATTGTTCTTGTCCACTCATTTTTATCAACATCTTGGCTGATCTATGATTTGGAAACATAGGTGATGACTGCATTTCAATCGGGCCCGGCTGTGCCAACGTAGACATTGAAGGCGTCACTTGTGGGCCGAGTCATGGAATCAGGTACAAACAACGTCGATTGCGATCTCCATTTATTTGTTTATCACTAGTCAACGCAGTGAATAATTCATTGTTGTACGTAGCAGCATTGGGAGTCTGGGAGTCCACAATTCACAGGCATGCGTTTCCAACATAACAGTGAGAAATGCAGTGATTAGGGAATCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGTACAGTGAGCGACATCTTATTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAGGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACGAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGC

mRNA sequence

ACTAATATATATATATATGTGCTACACAACAACATAGGTTCACGAGACTAATGAACTCTGATGTACCTTTTTCTCTTTCTTTTCTCTCCCTTCCACTAACCAAAACCAACATGGACCATTGGCTTTACTTCCCATTCTGTTTTAACCCATCTTCTGCTATTTCACCTTTCTTCACACCATGCATTTCTACATAAATATTTCTTTGTCCTCTAAACCCCTCCACTTTTTTACTTTTTACTATAATATCCTGCTCATCTCTCTCTCTCTCAACTCGACACCTGTTCTCTTTCTACACGTTACTTCTTTCCTTTCTCTTATAAAGTATTAACAAATGTAGATACATATGCATATATAGCTGCTTCTGTCCACCCAGCTTCCCCACTTCATTCTAACCTTTGTTCTTTACAACTCAAGTCTCTTTCTCTCCATTCCTTTCCCATTCTTTCCTCCTGCCATCCCTCTCCATTTTTGCCACTTGTTGCTCACTGCTAACTTTCTGGCCTTGCATTCTTTTCAAATTCACCTGGCTTCCATGGAGTTCAATCGAGTTCCTGCTGTAATGCTTTGGTTCACCGCCATTTTGGTGGCGGACAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGCAAGACTCGGTTCCTTCAACTCCAGCTCACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGACGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTTGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGGCTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGACAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCATATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGTCCACAATTCACAGGCATGCGTTTCCAACATAACAGTGAGAAATGCAGTGATTAGGGAATCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGTACAGTGAGCGACATCTTATTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAGGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACGAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGC

Coding sequence (CDS)

ATGGAGTTCAATCGAGTTCCTGCTGTAATGCTTTGGTTCACCGCCATTTTGGTGGCGGACAACTTGAGCAATGTGCAAGGAAGATACCATTTTCACAAGGGACAAAAGAAACAGAACCCTGATGAACAGCCCTCTCTTCCATCCCCTGTTTATTCACCACCACCGCAAGACTCGGTTCCTTCAACTCCAGCTCACCCAGCTCCCTCAGTGCCATCTGATCCCTACCCTGATGATCCAGGAAACACCACTTCTGACTGTGTTTTTGACGTCACTAATTATGGGGCGGTTGGAGATGGTTGTGCTGATGACACTGCTGCCTTCAGGGCAGCATGGAAAGCAGCCTGTGCTGTTGAATCTGCCACTCTTTTAGTTCCCTCTGATCGTTGCTTTAAAATCACTTCAACTATCTTCTCAGGACCTTGCAAACCAGGACTTGTGTTTAAGGTGGATGGAACCCTGATGCCACCGGACGGACCAGAGTCGTGGCCGAAGGCGGACAGTTTGAGGCAATGGCTCGTGTTTTACAGGCTGGATCAAATGACATTGACTGGAACTGGAACCATCGAAGGGAATGGCCAAAAATGGTGGGAGTTGCCATGCAAACCACATAGGGGTCCAAATGGGTCGACTCTGCCAGGACCATGTGACAGCCCTGCGTTAATAAGGTTCTTCATGAGCAGTAATTTGGCAGTGAGTGGGCTGAGAATTCAGAACAGTCCACAGTTCCATATGAAATTCGATGGGTGTGAAGGAGTTCTGATAGACAAGCTGTCAATATCTTCCCCAAAGCTCAGTCCCAACACTGACGGAATCCATATAGAGAATACCAAAGGAGTAGGAATATACAACTCCATGATAGGCAATGTCCACAATTCACAGGCATGCGTTTCCAACATAACAGTGAGAAATGCAGTGATTAGGGAATCAGACAATGGAGTGAGGATAAAGACATGGCAAGGAGGGTCGGGTACAGTGAGCGACATCTTATTCGAGAACATACAGATGGAGAATGTTAGAAACTGCATCATAGTTGACCAGTACTACTGCTTGTCCAAGGATTGTCGTAACCAGACATCGGCTGTGTTTGTGAACCAAGTCTTGTACAAGAACATCAAGGGAACTTACGACGTTAGGAACACTCCAATTCACTTCGCGTGCAGCGACACAGTGGCCTGCACGAATATTACAATGTCTGAAGTAGAATTGCTACCACATGAGGGTGAATTGGTGGAAGATCCTTTCTGTTGGAATGCATTTGGGATTCAGGAGACTTTGACCATTCCTCCCATTGATTGCTTGCAAGAAGGAGCACCTCAGAATGTCGCAGAGACCTATGAATATAGCTGC

Protein sequence

MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVPSTPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC

Homology

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BLAST of Cp4.1LG17g00130 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 617.1 bits (1590), Expect = 1.6e-175
Identity = 305/480 (63.54%), Postives = 361/480 (75.21%), Query Frame = 0


Query: 9   VMLWFTAILVADNLSNV-QGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVPSTPAHPA 68
           ++++  + ++  + +NV + RYH+HKG+          +  P+  PPP    P   A+P 
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH--------GVTHPLPPPPPP---PLETANPP 67

Query: 69  PSVPSDPYPDD---PGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLV 128
             VPSDPYP     PG++ S CVFDVT++GAVGDG  DDTAAF+ AWKAACAVES  +L 
Sbjct: 68  DQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLA 127

Query: 129 PSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTG 188
           P    FKITSTIFSGPCKPGLVF++DG LMPPDGPE WP+ D+  QWLVFYRLD  T +G
Sbjct: 128 PEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSG 187

Query: 189 TGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHM 248
            GT+EGNGQKWW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V GLRIQNSPQFHM
Sbjct: 188 KGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHM 247

Query: 249 KFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN---------------- 308
           KFDGC+GVLI+++ ISSPKLSPNTDGIH+ NT+ VGIYNS++ N                
Sbjct: 248 KFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDI 307

Query: 309 -----------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFE 368
                            VHNSQACVSNITVRN VIR+SDNG+R+KTWQGG+G+VS++LFE
Sbjct: 308 QGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFE 367

Query: 369 NIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVA 428
           NIQMENV NCIIVDQYYC SKDCRN+TSAV V  V Y+NIKGTYDVR+ PIHFACSDTVA
Sbjct: 368 NIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVA 427

Query: 429 CTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE--YSC 450
           CTNITMSEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G+P  V E Y+  Y C
Sbjct: 428 CTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPV-VEEAYDSNYGC 475

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BLAST of Cp4.1LG17g00130 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 261.5 bits (667), Expect = 1.7e-68
Identity = 148/388 (38.14%), Postives = 209/388 (53.87%), Query Frame = 0


Query: 84  SDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESAT-LLVPSDRCFKITSTIFSGPCK 143
           S+ +  V N+GA G+G  DDT AF  AWK AC+ +  T +LVP +    +     SGPCK
Sbjct: 49  SERLLHVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRILVPENYTCLLRPIDLSGPCK 108

Query: 144 PGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKP 203
             L  ++ GT++ P+ P+ W +  + R+WL F+ L ++T+ G GT+ G GQ+WWE  CK 
Sbjct: 109 ARLTLQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK- 168

Query: 204 HRGPNGSTLPGPC-DSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISS 263
           H   N      PC  +P  + F    N+ V  L + +S Q H+    C  V I  L + +
Sbjct: 169 HNHSN------PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIA 228

Query: 264 PKLSPNTDGIHIENTKGVGIYNSMIGN-------VHNS-QACVSN--------------- 323
           P  SPNTDGIHI  ++G+ I N+ +         V NS Q  +SN               
Sbjct: 229 PATSPNTDGIHISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLG 288

Query: 324 ----------ITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYY 383
                     ITV  A+I ++ NGVRIKTWQGGSG VS I+F NI+M NV N II+DQYY
Sbjct: 289 KSKSWEEVRDITVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYY 348

Query: 384 CLS-KDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEG 436
           C S K C NQTSA+ +  + + +++GT       I  +CSD+  C NI + +++L P  G
Sbjct: 349 CDSRKPCANQTSAISIENISFVHVRGT-SASKEAIKISCSDSSPCRNILLQDIDLEPSNG 408

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BLAST of Cp4.1LG17g00130 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 260.8 bits (665), Expect = 2.9e-68
Identity = 154/397 (38.79%), Postives = 215/397 (54.16%), Query Frame = 0


Query: 87  VFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLV 146
           +F+V  YGAVGDG  D T AF  AW+AAC   SA LLVP ++ F + +  F+GPC+P   
Sbjct: 58  IFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPSAMLLVPGNKKFVVNNLFFNGPCQPHFT 117

Query: 147 FKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGP 206
           FKVDG +     P SW    + R WL F +L   TL G G I+G G++WW   CK     
Sbjct: 118 FKVDGIIAAYQNPASW---KNNRIWLQFAKLTGFTLMGKGVIDGQGKQWWAGQCK---WV 177

Query: 207 NGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSP 266
           NG  +    D P  I+F  S+ L + GL++ NSP+FH+ F  CEGV I  +SI++P+ SP
Sbjct: 178 NGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIGISITAPRDSP 237

Query: 267 NTDGIHI---------ENTKGVG--------------IYNSMIGNVH----------NSQ 326
           NTDGI I         +NT G G              I + + G  H          NS+
Sbjct: 238 NTDGIDIFASKNFHLQKNTIGTGDDCVAIGTGSSNIVIEDLICGPGHGISIGSLGRENSR 297

Query: 327 ACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKD 386
           A VS + V  A   ++ NG+RIKTWQGGSG  S I++EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSYVHVNGAKFIDTQNGLRIKTWQGGSGMASHIIYENVEMINSENPILINQFYCTSAS 357

Query: 387 -CRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED 446
            C+NQ SAV +  V YKNI+GT       I   CSD++ C +I +S++ L    G++   
Sbjct: 358 ACQNQRSAVQIQDVTYKNIRGT-SATAAAIQLKCSDSMPCKDIKLSDISLKLTSGKIAS- 417

Query: 447 PFCW--NAFGIQETLTIPPIDCLQEGAPQNVAETYEY 448
             C   NA G      IP    L   A +  ++++++
Sbjct: 418 --CLNDNANGYFSGHVIPACKNLSPSAKRKESKSHKH 444

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BLAST of Cp4.1LG17g00130 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 1.8e-65
Identity = 145/350 (41.43%), Postives = 190/350 (54.29%), Query Frame = 0


Query: 87  VFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLV 146
           VF+V  YGAVGDG  D T AF   W AAC   SA LLVP+++ F + + +F GPC+P L 
Sbjct: 58  VFNVEQYGAVGDGKHDSTEAFATTWNAACKKASAVLLVPANKKFFVNNLVFRGPCQPHLS 117

Query: 147 FKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGP 206
           FKVDGT++    P  W  +   + WL F +L    L GTG I+G GQ+WW   CK     
Sbjct: 118 FKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQWWAGQCKV---V 177

Query: 207 NGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSP 266
           NG T+    + P  I+   S ++ V  L + NSP+FH+ F  CEGV I  L I +P+ SP
Sbjct: 178 NGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSP 237

Query: 267 NTDGIHIENTKGVGIYNSMIG-------------NV--------------------HNSQ 326
           NTDGI I  +K   I   +IG             N+                     NS+
Sbjct: 238 NTDGIDIFASKRFHIEKCVIGTGDDCIAIGTGSSNITIKDLICGPGHGISIGSLGRDNSR 297

Query: 327 ACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKD 386
           A VS++ V  A   ++ NG+RIKTWQGGSG  S I +EN++M N  N I+++Q+YC S  
Sbjct: 298 AEVSHVHVNRAKFIDTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSAS 357

Query: 387 -CRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVEL 403
            C+NQ SAV +  V YKNI GT       I   CSD+V CT I +S V L
Sbjct: 358 ACQNQRSAVQIQGVTYKNIHGT-SATAAAIQLMCSDSVPCTGIQLSNVSL 400

[Close]

BLAST of Cp4.1LG17g00130 vs. ExPASy Swiss-Prot
Match: P35336 (Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 9.7e-64
Identity = 144/386 (37.31%), Postives = 205/386 (53.11%), Query Frame = 0


Query: 89  DVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFK 148
           +V ++GA GDG  DDT AF  AWKAAC+  S+ +L+   + + +    FSGPCK GL  +
Sbjct: 91  NVDDFGAKGDG-RDDTKAFEKAWKAACSSTSSAVLLVPKKNYLVRPISFSGPCKSGLTMQ 150

Query: 149 VDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNG 208
           + GT+   D    + K    R WLVF  +  + + G GTI GNG+ WW+  CK ++    
Sbjct: 151 IYGTIEASDDRSDYRKDG--RHWLVFDSVQNLRVEGGGTINGNGKIWWQNSCKTNKAL-- 210

Query: 209 STLPGPC-DSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPN 268
                PC D+P  + F+ S ++ V  L+I+N+ Q H+ FD C  V    L +++P+ SPN
Sbjct: 211 -----PCKDAPTALTFYKSKHVIVKNLKIENAQQIHVSFDNCVNVQASNLMVTAPENSPN 270

Query: 269 TDGIHIENTKGVGIYNSMIG---------------------------------NVHNSQA 328
           TDGIH+  T+ + I + +IG                                    NS+A
Sbjct: 271 TDGIHVTGTQNIHISSCVIGTGDDCISIVNGSRKVRVNDITCGPGHGISIGSLGYGNSEA 330

Query: 329 CVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYC-LSKD 388
            VS++ V  A +  + NGVRIKTWQGGSG+ S+I F+N++M NV N II+DQ YC   K 
Sbjct: 331 HVSDVVVNGAKLCGTTNGVRIKTWQGGSGSASNIKFQNVEMHNVENPIIIDQNYCDQDKP 390

Query: 389 CRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDP 440
           C+ Q+SAV V  V+Y+NIKGT    N  I F CS    C  I + +V+ L  EG      
Sbjct: 391 CQEQSSAVQVKNVVYQNIKGTC-ASNVAITFDCSKRFPCQGIVLEDVD-LEIEGGAAAKA 450

[Close]

BLAST of Cp4.1LG17g00130 vs. NCBI nr
Match: XP_023514293.1 (polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 911 bits (2355), Expect = 0.0
Identity = 449/482 (93.15%), Postives = 449/482 (93.15%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP
Sbjct: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60

Query: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 449
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

[Close]

BLAST of Cp4.1LG17g00130 vs. NCBI nr
Match: KAG7024752.1 (Polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 900 bits (2327), Expect = 0.0
Identity = 444/483 (91.93%), Postives = 446/483 (92.34%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRVPAVMLW TAILVA+NLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPP+DSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPA PAP VPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPPVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ----------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVS 360
                                 VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVS
Sbjct: 301 NVDIEGVTCGPSHGISSIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVS 360

Query: 361 DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC 420
           DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC
Sbjct: 361 DILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFAC 420

Query: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE 449
           SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE
Sbjct: 421 SDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE 480

[Close]

BLAST of Cp4.1LG17g00130 vs. NCBI nr
Match: XP_022930280.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 443/482 (91.91%), Postives = 446/482 (92.53%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRVPAVMLW TAILVA+NLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPP+DSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPA PAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 449
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

[Close]

BLAST of Cp4.1LG17g00130 vs. NCBI nr
Match: XP_023000015.1 (polygalacturonase At1g48100-like [Cucurbita maxima])

HSP 1 Score: 898 bits (2320), Expect = 0.0
Identity = 442/482 (91.70%), Postives = 445/482 (92.32%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRVPAVMLW TAILVA+NLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPP+DS P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPA  APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADQAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQ+LYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 449
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

[Close]

BLAST of Cp4.1LG17g00130 vs. NCBI nr
Match: KAG6594032.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 887 bits (2293), Expect = 0.0
Identity = 433/449 (96.44%), Postives = 435/449 (96.88%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRVPAVMLW TAILVA+NLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPP+DSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPA PAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNVHNSQACVSNIT 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNV           
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNV----------- 300

Query: 301 VRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSA 360
            RNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSA
Sbjct: 301 -RNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSA 360

Query: 361 VFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFG 420
           VFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFG
Sbjct: 361 VFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFG 420

Query: 421 IQETLTIPPIDCLQEGAPQNVAETYEYSC 449
           IQETLTIPPIDCLQEGAPQNVAETYEYSC
Sbjct: 421 IQETLTIPPIDCLQEGAPQNVAETYEYSC 437

[Close]

BLAST of Cp4.1LG17g00130 vs. ExPASy TrEMBL
Match: A0A6J1EWI8 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 PE=3 SV=1)

HSP 1 Score: 899 bits (2323), Expect = 0.0
Identity = 443/482 (91.91%), Postives = 446/482 (92.53%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRVPAVMLW TAILVA+NLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPP+DSVP
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSVP 60

Query: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPA PAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSSRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 449
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

[Close]

BLAST of Cp4.1LG17g00130 vs. ExPASy TrEMBL
Match: A0A6J1KH63 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=3 SV=1)

HSP 1 Score: 898 bits (2320), Expect = 0.0
Identity = 442/482 (91.70%), Postives = 445/482 (92.32%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRVPAVMLW TAILVA+NLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPP+DS P
Sbjct: 1   MEFNRVPAVMLWITAILVAENLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPEDSAP 60

Query: 61  STPAHPAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120
           STPA  APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA
Sbjct: 61  STPADQAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESA 120

Query: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180
           TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM
Sbjct: 121 TLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQM 180

Query: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240
           TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP
Sbjct: 181 TLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSP 240

Query: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN------------ 300
           QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN            
Sbjct: 241 QFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGNGDDCISIGPGCA 300

Query: 301 ---------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360
                                VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD
Sbjct: 301 NVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSD 360

Query: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACS 420
           ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQ+LYKNIKGTYDVRNTPIHFACS
Sbjct: 361 ILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQILYKNIKGTYDVRNTPIHFACS 420

Query: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 449
           DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY
Sbjct: 421 DTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEY 480

[Close]

BLAST of Cp4.1LG17g00130 vs. ExPASy TrEMBL
Match: A0A6J1CB48 (polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111009879 PE=3 SV=1)

HSP 1 Score: 830 bits (2144), Expect = 5.50e-302
Identity = 409/490 (83.47%), Postives = 431/490 (87.96%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRV A+++  TAIL+ +N SNVQGRYHFHKGQKKQ PDE PS+PSPVYSPPP+++VP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  STPAHPAPS--------VPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWK 120
           S+PA PAPS        VPSDPYPDDP N+TSDCVFDVT+YGAVGDGC DDTAAFRAAWK
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDPRNSTSDCVFDVTDYGAVGDGCTDDTAAFRAAWK 120

Query: 121 AACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWL 180
           AACAVESAT+LVPSD CF ITSTIFSGPCKPGLVFKVDG+LMPPDGPESWPKADS+RQWL
Sbjct: 121 AACAVESATILVPSDHCFIITSTIFSGPCKPGLVFKVDGSLMPPDGPESWPKADSVRQWL 180

Query: 181 VFYRLDQMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVS 240
           VFYRLDQMT+TGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNL VS
Sbjct: 181 VFYRLDQMTMTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLVVS 240

Query: 241 GLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN---- 300
           GLRIQNSPQFHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMI N    
Sbjct: 241 GLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGDDC 300

Query: 301 -----------------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQ 360
                                        VHNSQACVSNITVRNAVIR+SDNGVRIKTWQ
Sbjct: 301 ISIGPGCANVDIEGVTCGPSHGISIGSLGVHNSQACVSNITVRNAVIRDSDNGVRIKTWQ 360

Query: 361 GGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRN 420
           GGSG+VSDILFENIQMENVRNCIIVDQYYCLSK C NQTSAVFVNQVLY+NIKGTYDVRN
Sbjct: 361 GGSGSVSDILFENIQMENVRNCIIVDQYYCLSKGCLNQTSAVFVNQVLYRNIKGTYDVRN 420

Query: 421 TPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQ 449
           TPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNA+GIQETLTIPPIDCLQ+G PQ
Sbjct: 421 TPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAYGIQETLTIPPIDCLQDGQPQ 480

[Close]

BLAST of Cp4.1LG17g00130 vs. ExPASy TrEMBL
Match: A0A6J1CE60 (polygalacturonase At1g48100 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111009879 PE=3 SV=1)

HSP 1 Score: 815 bits (2106), Expect = 9.27e-296
Identity = 409/517 (79.11%), Postives = 431/517 (83.37%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           MEFNRV A+++  TAIL+ +N SNVQGRYHFHKGQKKQ PDE PS+PSPVYSPPP+++VP
Sbjct: 1   MEFNRVLAILVCITAILLVENSSNVQGRYHFHKGQKKQKPDEDPSVPSPVYSPPPEEAVP 60

Query: 61  STPAHPAPS--------VPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWK 120
           S+PA PAPS        VPSDPYPDDP N+TSDCVFDVT+YGAVGDGC DDTAAFRAAWK
Sbjct: 61  SSPAEPAPSPPAEPAPSVPSDPYPDDPRNSTSDCVFDVTDYGAVGDGCTDDTAAFRAAWK 120

Query: 121 AACAVESATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWL 180
           AACAVESAT+LVPSD CF ITSTIFSGPCKPGLVFKVDG+LMPPDGPESWPKADS+RQWL
Sbjct: 121 AACAVESATILVPSDHCFIITSTIFSGPCKPGLVFKVDGSLMPPDGPESWPKADSVRQWL 180

Query: 181 VFYRLDQMTLTGTGTIEGNGQKWWELPCKPHR---------------------------G 240
           VFYRLDQMT+TGTGTIEGNGQKWWELPCKPHR                           G
Sbjct: 181 VFYRLDQMTMTGTGTIEGNGQKWWELPCKPHRVGYPHYIYPLHYHSFSFNQRLNYFSLQG 240

Query: 241 PNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLS 300
           PNGSTLPGPCDSPALIRFFMSSNL VSGLRIQNSPQFHMKFDGCEGVLI+KLSISSPKLS
Sbjct: 241 PNGSTLPGPCDSPALIRFFMSSNLVVSGLRIQNSPQFHMKFDGCEGVLIEKLSISSPKLS 300

Query: 301 PNTDGIHIENTKGVGIYNSMIGN---------------------------------VHNS 360
           PNTDGIHIENTKGVGIYNSMI N                                 VHNS
Sbjct: 301 PNTDGIHIENTKGVGIYNSMISNGDDCISIGPGCANVDIEGVTCGPSHGISIGSLGVHNS 360

Query: 361 QACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSK 420
           QACVSNITVRNAVIR+SDNGVRIKTWQGGSG+VSDILFENIQMENVRNCIIVDQYYCLSK
Sbjct: 361 QACVSNITVRNAVIRDSDNGVRIKTWQGGSGSVSDILFENIQMENVRNCIIVDQYYCLSK 420

Query: 421 DCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED 449
            C NQTSAVFVNQVLY+NIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED
Sbjct: 421 GCLNQTSAVFVNQVLYRNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGELVED 480

[Close]

BLAST of Cp4.1LG17g00130 vs. ExPASy TrEMBL
Match: A0A0A0KB54 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1)

HSP 1 Score: 796 bits (2055), Expect = 5.97e-289
Identity = 396/467 (84.80%), Postives = 416/467 (89.08%), Query Frame = 0


Query: 1   MEFNRVPAVMLWFTAILVADNLSNVQGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVP 60
           ME N V  VM+W  AIL  +N SNV GRYHFHKG+K    DE  S+ SP     P+++VP
Sbjct: 1   MELNGVFIVMIWVVAILFVENSSNVNGRYHFHKGKK----DEDSSVSSP-----PENAVP 60

Query: 61  STPAHPAPSVPSDPYPDDPG--NTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVE 120
           STPA P PSVPSDPYP+DPG  N+TSDCVFDVT++GAVGDGC DDTAAF+AAWKAACAVE
Sbjct: 61  STPAQPLPSVPSDPYPNDPGTGNSTSDCVFDVTDFGAVGDGCTDDTAAFKAAWKAACAVE 120

Query: 121 SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLD 180
           SATLLVPS+ CFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADS RQWLVFYRLD
Sbjct: 121 SATLLVPSNLCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSPRQWLVFYRLD 180

Query: 181 QMTLTGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQN 240
           QMTLTG+GTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAV+ LRIQN
Sbjct: 181 QMTLTGSGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVNSLRIQN 240

Query: 241 SPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN---------- 300
           SP FHMKFDGCEGVLI+KLSISSPKLSPNTDGIHIENTKGVGIYNSMI N          
Sbjct: 241 SPMFHMKFDGCEGVLIEKLSISSPKLSPNTDGIHIENTKGVGIYNSMISNGVTCGPSHGI 300

Query: 301 ------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFENIQMENVRNCI 360
                 VHNSQACVSNITVRNAVIR+SDNG+RIKTWQGGSG+VSDILFENIQMENVRNCI
Sbjct: 301 SIGSLGVHNSQACVSNITVRNAVIRDSDNGLRIKTWQGGSGSVSDILFENIQMENVRNCI 360

Query: 361 IVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVACTNITMSEVEL 420
           IVDQYYCLSKDC NQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSD+VACTNITMSEVEL
Sbjct: 361 IVDQYYCLSKDCLNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDSVACTNITMSEVEL 420

Query: 421 LPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYSC 449
           LPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEG PQNVAET EYSC
Sbjct: 421 LPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGEPQNVAETSEYSC 458

[Close]

BLAST of Cp4.1LG17g00130 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 617.1 bits (1590), Expect = 1.1e-176
Identity = 305/480 (63.54%), Postives = 361/480 (75.21%), Query Frame = 0


Query: 9   VMLWFTAILVADNLSNV-QGRYHFHKGQKKQNPDEQPSLPSPVYSPPPQDSVPSTPAHPA 68
           ++++  + ++  + +NV + RYH+HKG+          +  P+  PPP    P   A+P 
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH--------GVTHPLPPPPPP---PLETANPP 67

Query: 69  PSVPSDPYPDD---PGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLV 128
             VPSDPYP     PG++ S CVFDVT++GAVGDG  DDTAAF+ AWKAACAVES  +L 
Sbjct: 68  DQVPSDPYPSPDPAPGDSDSGCVFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESGVVLA 127

Query: 129 PSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTG 188
           P    FKITSTIFSGPCKPGLVF++DG LMPPDGPE WP+ D+  QWLVFYRLD  T +G
Sbjct: 128 PEGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSG 187

Query: 189 TGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQFHM 248
            GT+EGNGQKWW+LPCKPHRGP+GS+  GPC SP +IRFFMS+N+ V GLRIQNSPQFHM
Sbjct: 188 KGTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHM 247

Query: 249 KFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN---------------- 308
           KFDGC+GVLI+++ ISSPKLSPNTDGIH+ NT+ VGIYNS++ N                
Sbjct: 248 KFDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDVDI 307

Query: 309 -----------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDILFE 368
                            VHNSQACVSNITVRN VIR+SDNG+R+KTWQGG+G+VS++LFE
Sbjct: 308 QGVTCGPSHGISIGSLGVHNSQACVSNITVRNTVIRDSDNGLRVKTWQGGTGSVSNLLFE 367

Query: 369 NIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDTVA 428
           NIQMENV NCIIVDQYYC SKDCRN+TSAV V  V Y+NIKGTYDVR+ PIHFACSDTVA
Sbjct: 368 NIQMENVLNCIIVDQYYCQSKDCRNETSAVKVFDVEYRNIKGTYDVRSPPIHFACSDTVA 427

Query: 429 CTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYE--YSC 450
           CTNITMSEVELLP EGELV+DPFCWNA+G QETLTIPPIDCL +G+P  V E Y+  Y C
Sbjct: 428 CTNITMSEVELLPEEGELVDDPFCWNAYGKQETLTIPPIDCLLDGSPV-VEEAYDSNYGC 475

[Close]

BLAST of Cp4.1LG17g00130 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 489.2 bits (1258), Expect = 3.6e-138
Identity = 250/481 (51.98%), Postives = 319/481 (66.32%), Query Frame = 0


Query: 9   VMLWFTAILVADNLSNVQGRYH--FHKGQKKQNPDEQPSLPSPVYSPPPQDSVPSTPAHP 68
           V+ + T IL   +LS  +  +H   HK     +   +P  PS   S PP    P  P  P
Sbjct: 11  VLGFMTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPP-PGPPDSP 70

Query: 69  APSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESAT---LL 128
           APS+P  P  DDP +  +  +++V  YGAVGDG  DDT AF+ AW ++C  E+ T   LL
Sbjct: 71  APSLPPSP-SDDPADDNNG-IYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLL 130

Query: 129 VPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLT 188
           VP    F I STIF+GPC+    F+VDGT++ PDGPESWP   S RQWLVFYR++ M L 
Sbjct: 131 VPYGYTFMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALK 190

Query: 189 GTGTIEGNGQKWWELPCKPHRGPNGSTL-PGPCDSPALIRFFMSSNLAVSGLRIQNSPQF 248
           G G I+G GQKWW+LPCKPHR  N S +  GPCDSP  +RFFMSSNL V GL+I+NSPQF
Sbjct: 191 GEGVIDGRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQF 250

Query: 249 HMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN-------------- 308
           H +FDGC+GV ++ L I++P LSPNTDGIHIEN+  V IYNS+I N              
Sbjct: 251 HFRFDGCQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISNGDDCVSIGSGSYDV 310

Query: 309 --------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDI 368
                                HNS+ACVSNITVR++VI+ SDNGVRIKTWQGGSG+VS +
Sbjct: 311 DIRNLTCGPGGHGISIGSLGNHNSRACVSNITVRDSVIKYSDNGVRIKTWQGGSGSVSGV 370

Query: 369 LFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSD 428
            F NI +++VRN II+DQYYC++KDC N+TSAVFV+ + Y+ IKGTYD+R+ P+HF CSD
Sbjct: 371 TFNNIHVDSVRNPIIIDQYYCMTKDCANKTSAVFVSDIAYQGIKGTYDIRSPPMHFGCSD 430

Query: 429 TVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAPQNVAETYEYS 450
            V CTN+T+S++ELLP +GE+V DPFCWNA+GI E L+IPP+ CL    P+ +  +    
Sbjct: 431 AVPCTNLTLSDIELLPAKGEIVLDPFCWNAYGIAEELSIPPVWCLMSDPPKGLQGSLVDK 488

[Close]

BLAST of Cp4.1LG17g00130 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 434.9 bits (1117), Expect = 8.0e-122
Identity = 211/409 (51.59%), Postives = 277/409 (67.73%), Query Frame = 0


Query: 66  PAPSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAAC---AVESATL 125
           P+P+ P+  Y D+  +  +  VFDVT++GA+GD   DDT+AF+ AW AAC     +SA L
Sbjct: 29  PSPA-PNPAYNDN--DNIAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAACMSTGPKSALL 88

Query: 126 LVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTL 185
           LVP   CF +  T F+GPC+  LV ++DG ++ PDGP SWP ++  RQW++FYR++ +++
Sbjct: 89  LVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWP-SNYQRQWMMFYRVNGLSI 148

Query: 186 TGTGTIEGNGQKWWELPCKPHRGPNGSTLPGPCDSPALIRFFMSSNLAVSGLRIQNSPQF 245
            G+G I G GQKWW LPCKPH+G NG+T  GPCDSP  IR F SS + + G+   NS QF
Sbjct: 149 QGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRIQGINFMNSAQF 208

Query: 246 HMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN-------------- 305
           H++FD C  V++D + I +P  SPNTDGIHIENT  V I NSMI N              
Sbjct: 209 HVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDDCISIGAGCFNV 268

Query: 306 -------------------VHNSQACVSNITVRNAVIRESDNGVRIKTWQGGSGTVSDIL 365
                              VHNSQA VSNITV N+ I  SDNGVRIKTWQGGSG+VS I+
Sbjct: 269 DIKNVTCGPSHGISIGSLGVHNSQAYVSNITVTNSTIWNSDNGVRIKTWQGGSGSVSRIV 328

Query: 366 FENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDVRNTPIHFACSDT 425
           F NI M NVRN I++DQYYC + +C NQTSAV ++ VLY NIKGTYD+R+ PIHF CSD+
Sbjct: 329 FSNILMVNVRNPIMIDQYYCQTNNCANQTSAVIISDVLYANIKGTYDLRSPPIHFGCSDS 388

Query: 426 VACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQEGAP 439
           V CTN+T++EV+L P +G+ +E+PFCWNA+G  + +T+PP+ CL +  P
Sbjct: 389 VPCTNLTLTEVDLFPSKGQHLENPFCWNAYGSMK-ITVPPVYCLLDAPP 432

[Close]

BLAST of Cp4.1LG17g00130 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 430.6 bits (1106), Expect = 1.5e-120
Identity = 212/417 (50.84%), Postives = 277/417 (66.43%), Query Frame = 0


Query: 65  HPAPSVPS-DPYPDDPGNTTS-------DCVFDVTNYGAVGDGCADDTAAFRAAWKAACA 124
           H A   PS  P P+DP  + S       + V+DV  YGAVG+G ADDT +F+ AW +AC+
Sbjct: 34  HQASQPPSPSPNPNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAWDSACS 93

Query: 125 VE----SATLLVPSDRCFKITSTIFSGPCKPGLVFKVDGTLMPPDGPESWPKADSLRQWL 184
                 ++ L VP    F I STIF+GPC+    F+VDGT++P DGP+SWP + + RQWL
Sbjct: 94  NNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLNKRQWL 153

Query: 185 VFYRLDQMTLTGTGTIEGNGQKWWELPCKPH-RGPNGSTLPGPCDSPALIRFFMSSNLAV 244
            FYR++ M L G G I+G GQ WW+LPCKPH +  N + L GPC+SPA +RFFMSSN+ V
Sbjct: 154 AFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMSSNVIV 213

Query: 245 SGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGIYNSMIGN--- 304
            GL I+NSPQ H+K DGC  V I+ L I SP  SPNTDGIHIEN+  V IYNS+I N   
Sbjct: 214 KGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVISNGDD 273

Query: 305 -------------------------------VHNSQACVSNITVRNAVIRESDNGVRIKT 364
                                            NS ACVSN+TVR++ I+ S+NGVRIKT
Sbjct: 274 CVSIGPGAYDIDIRNITCGPGGHGISIGSLGEKNSHACVSNVTVRDSFIKFSENGVRIKT 333

Query: 365 WQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLYKNIKGTYDV 424
           WQGGSG+VS + F+NI ++ VRN II+DQYYC +K C N+TSAVFVN ++Y++IKGTYD+
Sbjct: 334 WQGGSGSVSGVTFDNIHVDTVRNPIIIDQYYCTTKSCANKTSAVFVNDIVYQSIKGTYDI 393

Query: 425 RNTPIHFACSDTVACTNITMSEVELLPHEGELVEDPFCWNAFGIQETLTIPPIDCLQ 435
           R+ P+HF CS+ V CTN+T+S +ELLP + ++V  PFCWNA+GI +  ++P I CL+
Sbjct: 394 RSPPMHFGCSNNVPCTNLTLSNIELLPSKEDIVVGPFCWNAYGITDEFSVPLISCLK 450

[Close]

BLAST of Cp4.1LG17g00130 vs. TAIR 10
Match: AT1G60590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 4.6e-85
Identity = 190/491 (38.70%), Postives = 264/491 (53.77%), Query Frame = 0


Query: 18  VADNLSNVQGRYH-------FHKGQKKQNPDEQPSLP---SPVYSPPPQDSVPSTPAHPA 77
           ++D+LS+ + + H        HK + K     QP      SPV S PPQ   P  P H  
Sbjct: 48  LSDSLSSKKPKSHENNHHHMSHKSKPKPKMKTQPPKSNDGSPVVSQPPQVQQP-PPPHVQ 107

Query: 78  PSVPSDPYPDDPGNTTSDCVFDVTNYGAVGDGCADDTAAFRAAWKAACAVESATLLVPSD 137
           P  P  P P  P   +    F+V ++GA GDG +DDT AF AAW +AC VE++T+++P D
Sbjct: 108 P--PPTPLPLQPVEDSQQ--FNVLDFGAKGDGMSDDTQAFEAAWASACKVEASTMIIPPD 167

Query: 138 RCFKITSTIFSGP-CKPGLVFKVDGTLMPPDGPESWPKADSLRQWLVFYRLDQMTLTGTG 197
             F +    FSGP C+  +VF+++G ++ P   ESW     L  W+ F +L  +T+ G G
Sbjct: 168 YIFLVGPISFSGPYCQANIVFQLEGMIVAPTDTESW--GGGLMWWIEFTKLSGITIQGNG 227

Query: 198 TIEGNGQKWW-------------------------ELPCKPHRGPNGSTLPGPCDSPALI 257
            I+G G  WW                         E P  P R      +P     P  +
Sbjct: 228 VIDGRGTVWWQQDYLSDYPIDDDFKLIVPLNNSVQERPPMPIRSELNWRMPS--IKPTAL 287

Query: 258 RFFMSSNLAVSGLRIQNSPQFHMKFDGCEGVLIDKLSISSPKLSPNTDGIHIENTKGVGI 317
           RF+ S ++ V+G+ IQNSPQ H+KFD C  VL+  +++SSP  SPNTDGIH++NT+ V I
Sbjct: 288 RFYGSIDVTVTGITIQNSPQCHLKFDNCVKVLVHDVNVSSPGDSPNTDGIHLQNTRDVMI 347

Query: 318 YNSMIG---------------NVHN------------------SQACVSNITVRNAVIRE 377
           + + +                 VHN                  ++ACVSNITVR+ V+  
Sbjct: 348 HTTTLACGDDCISIQTGCSNVYVHNVNCGPGHGISIGSLGKDSTKACVSNITVRDVVMHN 407

Query: 378 SDNGVRIKTWQGGSGTVSDILFENIQMENVRNCIIVDQYYCLSKDCRNQTSAVFVNQVLY 437
           +  GVRIKTWQGG G+V  ILF NIQ+  V+  I++DQ+YC    C N TSAV V  V Y
Sbjct: 408 TMTGVRIKTWQGGIGSVKGILFSNIQLTEVQLPIVIDQFYCDHSKCMNHTSAVSVEGVTY 467

Query: 438 KNIKGTYDVRNTPIHFACSDTVACTNITMSEVELLPHEGEL-VEDPFCWNAFGIQETLTI 439
           + I+GTY V+  P+HFACSD+  C ++ +S +EL P + +  + DPFCW  FG   + T+
Sbjct: 468 EKIRGTYTVK--PVHFACSDSFPCIDVQLSGIELKPVQLQYHMYDPFCWKTFGELNSATV 527

The following BLAST results are available for this feature:

BLAST of Cp4.1LG17g00130 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	1.6e-175	63.54	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	1.7e-68	38.14	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P43212	2.9e-68	38.79	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q7M1E7	1.8e-65	41.43	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
P35336	9.7e-64	37.31	Polygalacturonase OS=Actinidia deliciosa OX=3627 PE=2 SV=1	[more]

BLAST of Cp4.1LG17g00130 vs. NCBI nr


Match Name	Identity	Description
XP_023514293.1	93.15	polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo]	[more]
KAG7024752.1	91.93	Polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022930280.1	91.91	polygalacturonase At1g48100-like [Cucurbita moschata]	[more]
XP_023000015.1	91.70	polygalacturonase At1g48100-like [Cucurbita maxima]	[more]
KAG6594032.1	96.44	Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Cp4.1LG17g00130 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EWI8	0.0	91.91	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111436782 P...	[more]
A0A6J1KH63	0.0	91.70	polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111494324 PE=...	[more]
A0A6J1CB48	5.50e-302	83.47	polygalacturonase At1g48100 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A6J1CE60	9.27e-296	79.11	polygalacturonase At1g48100 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1110...	[more]
A0A0A0KB54	5.97e-289	84.80	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G000120 PE=3 SV=1	[more]

BLAST of Cp4.1LG17g00130 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G48100.1	1.1e-176	63.54	Pectin lyase-like superfamily protein	[more]
AT1G02460.1	3.6e-138	51.98	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	8.0e-122	51.59	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	1.5e-120	50.84	Pectin lyase-like superfamily protein	[more]
AT1G60590.1	4.6e-85	38.70	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 296..317 e-value: 11.0 score: 15.0 coord: 249..275 e-value: 980.0 score: 7.3 coord: 325..346 e-value: 390.0 score: 9.8
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 119..288 e-value: 9.8E-28 score: 97.1 coord: 292..402 e-value: 9.0E-31 score: 107.1
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 80..446 e-value: 3.8E-93 score: 314.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 46..75
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 31..83
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 8..288 coord: 289..440
None	No IPR available	PANTHER	PTHR31375:SF142	POLYGALACTURONASE PLANT-LIKE PROTEIN	coord: 8..288 coord: 289..440
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 86..442

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG17g00130.1	Cp4.1LG17g00130.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0004650	polygalacturonase activity