Cp4.1LG15g01990 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG15g01990
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	E3 ubiquitin-protein ligase RNF14
Location	Cp4.1LG15: 1638593 .. 1641957 (-)
RNA-Seq Expression	Cp4.1LG15g01990
Synteny	Cp4.1LG15g01990

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

CACCATTGCCTGTGTGATCAAGTATGCATGAAGAAAAAATCGAAATTGCACATTTTTTAAACCTAAATTCAGTTCAAGGCTTCCGATTTGTGAAGGACGTGCAATGTGTAGAGTTGATTTGAGGCGATAATTCAAACGAAAATTCTGTTCGATTGCTATGATTTCATAATTCTCGTAGTCGTCGTCTGCGTAAATCTAGTAACATATCAACGTTTGGCAGTTGAATTCGAGTCTTCCGATCGAAATCTTGTATCCTTTGCCGCCTCTAATCCGACCTAACGTCTGCCGGAATTTGCAATGGGAAAGACCGGAAGAGGAGGTCGGCGGGCTGATCAGAATCGAACTCAAGAGGTTTGGGTTGCCAAACACTCGAATCAAACTCAGAATCGCGCTTCTACCTCCGGCTCACAACGCTTGGAGCCCGGTAATCGTCCTTCTACTGGTCGTAATTCCAACTACGAGCATGAGAATCGCCGCCGCCCCCGTCCTCGACCTGTCGAGAAAGCGGAGGTGAGTGCTTTAGCCGATGGGATAGAATCTTTCACTTGCGACGAAAAGCCTGTGGAAATGGAGGAGAGATGCGGAGATGTTGTGAATTCGAACTGTGAGATTAGAGACGAAACAGCTGGAAATTCTGGCTCAAGTGAGGCTACGGATGATGTTTACAGTAGGTTGGAGATGCTGCAACGCATCTCCGAGGAGCCGGAGTTGCCGGAGGAGCAGCTTAGGATCAATGATCAGTTGCAGGAAGATGAGGTATGCGATTAACGTTATTTGCCTCTGATTTGTAAGCGTAATTGATTGTTTTTCAGCTGAAGCTTGTTTATACACGTTAAATTTGCTACGAATTGATATTGGCGATGACATTTTTGTTCTTCATATTTATTCTTCGCGTTCAATCTTGAACTGTTTACTGTTTTATCGGTGAATCTTCGGCAGTTGCTGGCTCTGGAGTCCATATATGGAGAAAATATCCATATCCTCGATGAACATAGAGGGCAGCGCCGTTTCCAGGTTCATAACTATCCGATAAATTCATAATTGTTGCATCAAAACAATTTGGAATTTGATCCTCAAACTTTTCTATTATTCTGTAGATTTCCATACACATTGAGTATCCTGGTGATATTACTGTTACTGCAAATCTCAACTCATCTGGTGCTCTTGAGATGAAAAGAACAAATTCAGATGATTTCTCTTGTTCTTTCAAAGTCAAGTATCTTAATCCCATTGTTTTGACCTGTTTATTACCGAAATCGTATCCGAGTCACCTACCTCCATATTATACAATCTCTATTCAGTGGTTGGACGCTGCTAGAATTTCCCGTCTTTGCTCTGTATTGGATACGATATGGACGGAACAAGCGGGGCAGGAAGTTGTATATCAATGGGTAGAATGTCTACAAACGTCATCTCTGGTTTATCTTGAGATAGATAAAGAGATTACGCTTGGTCCTTACAACAAGGGACATTTGGGAGATATTCGAGCAGTTTCGGGAAGTGTTTCTCCAGAAGTTGATGTTCCTGCCATTGTAAATTATGACAATCAGAGACGTGAGGAGGACTTCTGCATGAACTTGCAGGAATGCTGCATCTGTTTAAGTCAGTTTGCAGGTAAGCTCAATAATTGGCTCATGATAAGATGCTATTATTCAATTTACAGCCTACTGACTCGTTCTACGTTCTACTTTTGCTTGTATGCATTAGCGAGAATAGTTTTAATAACAATCACTTATGACATAGTAATTAAGTTCAAGCATCTAATTTTTGGACATGGTGTCGGTTAACTAGCTTTGCCCTTCCCTCCCTTCCCTTCCCGACGCTAGAGAGAGTTGTATTTCGAAAGAGAAGACAAATTATCTGTACACAATTTGACTGACTATCCTACTCTAAGAAGAGGTTCCTTTGTTAGTCAGACCAGCAATATCTGCCTCATTATGTACCGAATATTACTCGGCCTGTTTATTATGGATTCTTGTTCGACTCCGCATATAGGTGTCAACTTTTGTTACATAATCACTAATTATCTGCATTAACTAAAAGATTCTCTTTGTTCCGGGTTGAATCACCCTAAGCGATGTTTTGATATTGGAGATATGAATGGGGAAAAAGTTCTAATCTGATATCCTTGTGGAATAGAACCACTCGCTTCAAACTTCAAAGAAAACTATTGTACTTCTTAAAACCTATTAAATGGAACCACGAACCACGAACTTAGCTGCTCCAAGTTGACAAAAATCTTTTGTAGTCATGGTAGCCTGACCTTTAATACGTCTTTCAGTCTGGGTGTAGAAATCGAACTGTCGAATGGGAACTTTTAACTCCAGCTGCTTTGTTTGTTGGTTGTGCATAATCAGGTACAAAGTTTGTAAGATTTCCATGTAAGCATTACTTTTGCTGGAAATGCATGGAGACATACTCAAATATGCATGTCAAGGAAGGCACCGTCAGCAAACTCAAATGTCCAGATGCCAAATGCGATCTTATGCTTCCTCCTGGTCTTTTGAAGCAATTGCTTGGTGCTGAGGAGTTTGAAAGATGGGAATCCATGTTGCTCACGAAAACACTGGAGTCAATGTCCGATGTAGTCTATTGCCCAAGGTGTGAAACCCCCTGCCTCGAGGACGTAGATCATGATGCTCAATGCTCGAAGTGTTACTTTAGTTTTTGTACACTCTGTAGCGAGAGACGTCATGTCGGAATTGAATGTATGACTCCAGAAATGAAGCTTCGTCTTCTTGAGGTTAGCTAAGTACCCAGTTCACCAATCCATTAGCATTTTTCCTTTTTGTGGGGATACTCATTAGCATTTATCGTTAGAACTCGACCTCTTACATTTCTTAACCTCGTTAACGTGTTATTCTTAATGCTGCAGGAGCGTCAAAGTTCATCTCAGTTAAGTAGTGATCAAAGACGAAAGGAACGTGAGATGATAAATGAGCTTATGAGTGTCAAGGAAATACTTCGCGATGCCAAGCAATGCCCATCTTGCAAAATGGCAATATCACGGACCGAAGGTTGCAATAAGATGGTTTGCAATAACTGTGGACAGTATTTCTGTTACCGCTGCAGCAAGGCAATTGATGGATATGACCATTTCAGGTTTGTTCGTGCGAACGAGCATTAGTTTAGTACTGACGATTCGCATCAATTGTGCACTTCTCTTATATAGATGAATCTAACACGCTAATCTTTCATGAAGGGAGGGCACATGTGAATTGTTTTCGCAAGAAACCATTCAGCAGTGGGAAGAGCGCATGAATGCACGGCAGCTGTTGGGCCAGATTCGTGCAGAACTTTTTCCTGAAAATGGTCATCCATGTCCTAATTGTGGTCAAATTAATGCCAAGGTGAGTAGCGAGTAG

mRNA sequence

CACCATTGCCTGTGTGATCAAGTATGCATGAAGAAAAAATCGAAATTGCACATTTTTTAAACCTAAATTCAGTTCAAGGCTTCCGATTTGTGAAGGACGTGCAATGTGTAGAGTTGATTTGAGGCGATAATTCAAACGAAAATTCTGTTCGATTGCTATGATTTCATAATTCTCGTAGTCGTCGTCTGCGTAAATCTAGTAACATATCAACGTTTGGCAGTTGAATTCGAGTCTTCCGATCGAAATCTTGTATCCTTTGCCGCCTCTAATCCGACCTAACGTCTGCCGGAATTTGCAATGGGAAAGACCGGAAGAGGAGGTCGGCGGGCTGATCAGAATCGAACTCAAGAGGTTTGGGTTGCCAAACACTCGAATCAAACTCAGAATCGCGCTTCTACCTCCGGCTCACAACGCTTGGAGCCCGGTAATCGTCCTTCTACTGGTCGTAATTCCAACTACGAGCATGAGAATCGCCGCCGCCCCCGTCCTCGACCTGTCGAGAAAGCGGAGGTGAGTGCTTTAGCCGATGGGATAGAATCTTTCACTTGCGACGAAAAGCCTGTGGAAATGGAGGAGAGATGCGGAGATGTTGTGAATTCGAACTGTGAGATTAGAGACGAAACAGCTGGAAATTCTGGCTCAAGTGAGGCTACGGATGATGTTTACAGTAGGTTGGAGATGCTGCAACGCATCTCCGAGGAGCCGGAGTTGCCGGAGGAGCAGCTTAGGATCAATGATCAGTTGCAGGAAGATGAGTTGCTGGCTCTGGAGTCCATATATGGAGAAAATATCCATATCCTCGATGAACATAGAGGGCAGCGCCGTTTCCAGATTTCCATACACATTGAGTATCCTGGTGATATTACTGTTACTGCAAATCTCAACTCATCTGGTGCTCTTGAGATGAAAAGAACAAATTCAGATGATTTCTCTTGTTCTTTCAAAGTCAAGTATCTTAATCCCATTGTTTTGACCTGTTTATTACCGAAATCGTATCCGAGTCACCTACCTCCATATTATACAATCTCTATTCAGTGGTTGGACGCTGCTAGAATTTCCCGTCTTTGCTCTGTATTGGATACGATATGGACGGAACAAGCGGGGCAGGAAGTTGTATATCAATGGGTAGAATGTCTACAAACGTCATCTCTGGTTTATCTTGAGATAGATAAAGAGATTACGCTTGGTCCTTACAACAAGGGACATTTGGGAGATATTCGAGCAGTTTCGGGAAGTGTTTCTCCAGAAGTTGATGTTCCTGCCATTGTAAATTATGACAATCAGAGACGTGAGGAGGACTTCTGCATGAACTTGCAGGAATGCTGCATCTGTTTAAGTCAGTTTGCAGGTACAAAGTTTGTAAGATTTCCATGTAAGCATTACTTTTGCTGGAAATGCATGGAGACATACTCAAATATGCATGTCAAGGAAGGCACCGTCAGCAAACTCAAATGTCCAGATGCCAAATGCGATCTTATGCTTCCTCCTGGTCTTTTGAAGCAATTGCTTGGTGCTGAGGAGTTTGAAAGATGGGAATCCATGTTGCTCACGAAAACACTGGAGTCAATGTCCGATGTAGTCTATTGCCCAAGGTGTGAAACCCCCTGCCTCGAGGACGTAGATCATGATGCTCAATGCTCGAAGTGTTACTTTAGTTTTTGTACACTCTGTAGCGAGAGACGTCATGTCGGAATTGAATGTATGACTCCAGAAATGAAGCTTCGTCTTCTTGAGGAGCGTCAAAGTTCATCTCAGTTAAGTAGTGATCAAAGACGAAAGGAACGTGAGATGATAAATGAGCTTATGAGTGTCAAGGAAATACTTCGCGATGCCAAGCAATGCCCATCTTGCAAAATGGCAATATCACGGACCGAAGGTTGCAATAAGATGGTTTGCAATAACTGTGGACAGTATTTCTGTTACCGCTGCAGCAAGGCAATTGATGGATATGACCATTTCAGGGAGGGCACATGTGAATTGTTTTCGCAAGAAACCATTCAGCAGTGGGAAGAGCGCATGAATGCACGGCAGCTGTTGGGCCAGATTCGTGCAGAACTTTTTCCTGAAAATGGTCATCCATGTCCTAATTGTGGTCAAATTAATGCCAAGGTGAGTAGCGAGTAG

Coding sequence (CDS)

ATGGGAAAGACCGGAAGAGGAGGTCGGCGGGCTGATCAGAATCGAACTCAAGAGGTTTGGGTTGCCAAACACTCGAATCAAACTCAGAATCGCGCTTCTACCTCCGGCTCACAACGCTTGGAGCCCGGTAATCGTCCTTCTACTGGTCGTAATTCCAACTACGAGCATGAGAATCGCCGCCGCCCCCGTCCTCGACCTGTCGAGAAAGCGGAGGTGAGTGCTTTAGCCGATGGGATAGAATCTTTCACTTGCGACGAAAAGCCTGTGGAAATGGAGGAGAGATGCGGAGATGTTGTGAATTCGAACTGTGAGATTAGAGACGAAACAGCTGGAAATTCTGGCTCAAGTGAGGCTACGGATGATGTTTACAGTAGGTTGGAGATGCTGCAACGCATCTCCGAGGAGCCGGAGTTGCCGGAGGAGCAGCTTAGGATCAATGATCAGTTGCAGGAAGATGAGTTGCTGGCTCTGGAGTCCATATATGGAGAAAATATCCATATCCTCGATGAACATAGAGGGCAGCGCCGTTTCCAGATTTCCATACACATTGAGTATCCTGGTGATATTACTGTTACTGCAAATCTCAACTCATCTGGTGCTCTTGAGATGAAAAGAACAAATTCAGATGATTTCTCTTGTTCTTTCAAAGTCAAGTATCTTAATCCCATTGTTTTGACCTGTTTATTACCGAAATCGTATCCGAGTCACCTACCTCCATATTATACAATCTCTATTCAGTGGTTGGACGCTGCTAGAATTTCCCGTCTTTGCTCTGTATTGGATACGATATGGACGGAACAAGCGGGGCAGGAAGTTGTATATCAATGGGTAGAATGTCTACAAACGTCATCTCTGGTTTATCTTGAGATAGATAAAGAGATTACGCTTGGTCCTTACAACAAGGGACATTTGGGAGATATTCGAGCAGTTTCGGGAAGTGTTTCTCCAGAAGTTGATGTTCCTGCCATTGTAAATTATGACAATCAGAGACGTGAGGAGGACTTCTGCATGAACTTGCAGGAATGCTGCATCTGTTTAAGTCAGTTTGCAGGTACAAAGTTTGTAAGATTTCCATGTAAGCATTACTTTTGCTGGAAATGCATGGAGACATACTCAAATATGCATGTCAAGGAAGGCACCGTCAGCAAACTCAAATGTCCAGATGCCAAATGCGATCTTATGCTTCCTCCTGGTCTTTTGAAGCAATTGCTTGGTGCTGAGGAGTTTGAAAGATGGGAATCCATGTTGCTCACGAAAACACTGGAGTCAATGTCCGATGTAGTCTATTGCCCAAGGTGTGAAACCCCCTGCCTCGAGGACGTAGATCATGATGCTCAATGCTCGAAGTGTTACTTTAGTTTTTGTACACTCTGTAGCGAGAGACGTCATGTCGGAATTGAATGTATGACTCCAGAAATGAAGCTTCGTCTTCTTGAGGAGCGTCAAAGTTCATCTCAGTTAAGTAGTGATCAAAGACGAAAGGAACGTGAGATGATAAATGAGCTTATGAGTGTCAAGGAAATACTTCGCGATGCCAAGCAATGCCCATCTTGCAAAATGGCAATATCACGGACCGAAGGTTGCAATAAGATGGTTTGCAATAACTGTGGACAGTATTTCTGTTACCGCTGCAGCAAGGCAATTGATGGATATGACCATTTCAGGGAGGGCACATGTGAATTGTTTTCGCAAGAAACCATTCAGCAGTGGGAAGAGCGCATGAATGCACGGCAGCTGTTGGGCCAGATTCGTGCAGAACTTTTTCCTGAAAATGGTCATCCATGTCCTAATTGTGGTCAAATTAATGCCAAGGTGAGTAGCGAGTAG

Protein sequence

MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRRRPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATDDVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQISIHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPYYTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYNKGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQINAKVSSE

Homology

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BLAST of Cp4.1LG15g01990 vs. ExPASy Swiss-Prot
Match: Q9JI90 (E3 ubiquitin-protein ligase RNF14 OS=Mus musculus OX=10090 GN=Rnf14 PE=1 SV=2)

HSP 1 Score: 218.8 bits (556), Expect = 1.7e-55
Identity = 145/463 (31.32%), Postives = 231/463 (49.89%), Query Frame = 0


Query: 148 QLQEDELLALESIYGENIHILDEHRGQRRFQ---ISIHIEYPGDITVTANLNSSGALEMK 207
           + QEDELLAL SIY       DE R     Q     I+++ P +  +  + NS+ +L+  
Sbjct: 7   EAQEDELLALASIYD-----ADEFRKAESVQGGETRIYLDLPQNFKIFVSGNSNESLQ-- 66

Query: 208 RTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPYYTISIQWLDAARISRLCSVLDTIW 267
                +    + + +L P+VL   LP  YPS  PP +T+S +WL   ++S LC  LD +W
Sbjct: 67  -----NSGFEYTICFLPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLW 126

Query: 268 TEQAGQEVVYQWVECLQTSSLVYLEIDK--EITLGPYNK------------GHLG----- 327
            E  G+ V++ W++ L+  +L YL I    E+ +G   K              LG     
Sbjct: 127 EEHRGRVVLFAWMQFLKEETLTYLNIVSPFELKMGSQKKVQRRATAQASSSTELGVGGAA 186

Query: 328 ----------DIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFV 387
                     D RAV    S    +  I++++  R+ + F   L  C IC  +  G+  +
Sbjct: 187 AADVDQEETVDERAVQDVESLSSLIQEILDFNQARQTKCFNSKLFLCSICFCEKLGSDCM 246

Query: 388 RF-PCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWES 447
            F  CKH +C  C++ Y  + +K+G V  L CP+ +C  +  PG +K+L+ A+ F R++ 
Sbjct: 247 YFLECKHVYCKACLKDYFEIQIKDGQVKCLNCPEPQCPSVATPGQVKELVEADLFARYDR 306

Query: 448 MLLTKTLESMSDVVYCPR--CETPCLEDVDHD-AQCSKCYFSFCTLCSERRHVGIEC-MT 507
           +LL  TL+ M+DVVYCPR  C+ P +++     A CS C F+FCTLC    H    C +T
Sbjct: 307 LLLQSTLDLMADVVYCPRPCCQLPVMQEPGGTMAICSSCNFAFCTLCRLTYHGLSPCKVT 366

Query: 508 PEMKLRLLEERQSSSQLSS---DQRRKER---EMINELMSVKEILRDAKQCPSCKMAISR 565
            E  + L  E   + + +    +QR  +R   + + E+ S   + +++K CP C   I +
Sbjct: 367 AEKLIDLRNEYLQADEATKRFLEQRYGKRVIQKALEEMESKDWLEKNSKSCPCCGTPIQK 426

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BLAST of Cp4.1LG15g01990 vs. ExPASy Swiss-Prot
Match: Q9UBS8 (E3 ubiquitin-protein ligase RNF14 OS=Homo sapiens OX=9606 GN=RNF14 PE=1 SV=1)

HSP 1 Score: 218.0 bits (554), Expect = 2.9e-55
Identity = 142/460 (30.87%), Postives = 232/460 (50.43%), Query Frame = 0


Query: 148 QLQEDELLALESIY-GENIHILDEHRGQRRFQISIHIEYPGDITVTANLNSSGALEMKRT 207
           + QEDELLAL SIY G+     +  +G    +  I+++ P +  +  + NS+  L+    
Sbjct: 7   EAQEDELLALASIYDGDEFRKAESVQGG---ETRIYLDLPQNFKIFVSGNSNECLQ---- 66

Query: 208 NSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPYYTISIQWLDAARISRLCSVLDTIWTE 267
              +    + + +L P+VL   LP  YPS  PP +T+S +WL   ++S LC  LD +W E
Sbjct: 67  ---NSGFEYTICFLPPLVLNFELPPDYPSSSPPSFTLSGKWLSPTQLSALCKHLDNLWEE 126

Query: 268 QAGQEVVYQWVECLQTSSLVYLEIDK--EITLGPYNK-----------------GHLG-- 327
             G  V++ W++ L+  +L YL I    E+ +G   K                 G  G  
Sbjct: 127 HRGSVVLFAWMQFLKEETLAYLNIVSPFELKIGSQKKVQRRTAQASPNTELDFGGAAGSD 186

Query: 328 -------DIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRF- 387
                  D RAV    S    +  I+++D  ++ + F   L  C IC  +  G++ + F 
Sbjct: 187 VDQEEIVDERAVQDVESLSNLIQEILDFDQAQQIKCFNSKLFLCSICFCEKLGSECMYFL 246

Query: 388 PCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLL 447
            C+H +C  C++ Y  + +++G V  L CP+ KC  +  PG +K+L+ AE F R++ +LL
Sbjct: 247 ECRHVYCKACLKDYFEIQIRDGQVQCLNCPEPKCPSVATPGQVKELVEAELFARYDRLLL 306

Query: 448 TKTLESMSDVVYCPR--CETPCLEDVDHD-AQCSKCYFSFCTLCSERRHVGIEC-MTPEM 507
             +L+ M+DVVYCPR  C+ P +++       CS C F+FCTLC    H    C +T E 
Sbjct: 307 QSSLDLMADVVYCPRPCCQLPVMQEPGCTMGICSSCNFAFCTLCRLTYHGVSPCKVTAEK 366

Query: 508 KLRLLEERQSSSQLSS---DQRRKER---EMINELMSVKEILRDAKQCPSCKMAISRTEG 565
            + L  E   + + +    DQR  +R   + + E+ S + + +++K CP C   I + +G
Sbjct: 367 LMDLRNEYLQADEANKRLLDQRYGKRVIQKALEEMESKEWLEKNSKSCPCCGTPIEKLDG 426

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BLAST of Cp4.1LG15g01990 vs. ExPASy Swiss-Prot
Match: Q04638 (Translation termination inhibitor protein ITT1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=ITT1 PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 1.3e-23
Identity = 75/267 (28.09%), Postives = 123/267 (46.07%), Query Frame = 0


Query: 342 CCICLSQFAGTKFVRFPC-----KHYFCWKCMETYSNMHVKEGTVSKLKCPD-------- 401
           CCIC+    G + ++ PC     +HY C  C ++Y    ++E  +S ++CP         
Sbjct: 180 CCICMEMEKGVRMIKLPCENANVEHYLCRGCAKSYFTAMIQENRISSVRCPQCEYKELKL 239

Query: 402 -----------AKCDLMLPPGLLKQLLGAEEFERWESMLLTKTLESMSD-----VVYCPR 461
                      A    ++P   LK+++  E  ER+E M   +    +S       V C R
Sbjct: 240 EDFKSYKKMLKALFTPLIPVSFLKEVIDTELCERYEKMFYNQAATRLSKYCPYACVTCRR 299

Query: 462 CETPCLEDVDHDA--QCSKCYFSFCTLCSERRH-VGIEC-MTPEMKLRLLEERQSSSQLS 521
           C++ C ++   DA  QC KC+F FC  C    H    +C     +   ++EE    +  S
Sbjct: 300 CDSWCTKEDLDDAMIQCQKCHFVFCFDCLHAWHGYNNKCGKKVSLSTDIIEEYLDDTVTS 359

Query: 522 SDQRRK----------EREMINEL------MSVKEILRDAKQCPSCKMAISRTEGCNKMV 557
            +++RK          E E+ + L      +++K+   + ++CP CK+ + R+EGCNKM 
Sbjct: 360 YERKRKLEAKYGRRVLELEVNDYLAEKMLDLAIKKEGSNLQRCPKCKVVVERSEGCNKMK 419

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BLAST of Cp4.1LG15g01990 vs. ExPASy Swiss-Prot
Match: Q54CX4 (Uncharacterized protein DDB_G0292642 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0292642 PE=4 SV=2)

HSP 1 Score: 92.8 bits (229), Expect = 1.4e-17
Identity = 55/209 (26.32%), Postives = 96/209 (45.93%), Query Frame = 0


Query: 341 ECCICLSQF-AGTKFVRFPCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGL 400
           EC IC  ++    +     C H +C+ C+  +  + + EG V  + CP  +C   +    
Sbjct: 615 ECKICYMEYDQSNEVFTLECDHVYCFDCITEHLRILITEGRVLDISCPHPQCKKEIKESE 674

Query: 401 LKQLLGAEEFERWESMLLTKTLESMSDVVYCPR--CETPCLEDVDHD--AQCSKCYFSFC 460
           +  L   + + +++   +  +L++   + +CP   C+TP     + +    C KC   FC
Sbjct: 675 IYMLTNEKNWLKYQKFSMIASLKT-EPIKWCPTPDCDTPVRGGSERNPILNCPKCSNDFC 734

Query: 461 TLCSERRHVGIECMTPEMKLRLLEERQSSSQLSSDQRRKEREMINELMSVKEILRDAKQC 520
            +C E  H G +C T  M+L+  + +   S  ++         I+ L S K  +   K C
Sbjct: 735 WICGEYSHEGAKCGTEAMELQGRKNKSIESAATA--------YIDFLESNKHFV---KPC 794

Query: 521 PSCKMAISRTEGCNKMVCNNCGQYFCYRC 545
           P+CK  I + +GCN M C NC   FC+ C
Sbjct: 795 PTCKSHIEKHDGCNHMTCINCQHQFCWLC 811

[Close]

BLAST of Cp4.1LG15g01990 vs. ExPASy Swiss-Prot
Match: A5PK27 (E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.0e-15
Identity = 58/237 (24.47%), Postives = 97/237 (40.93%), Query Frame = 0


Query: 339 LQECCICLSQFAGTKFVRF-PCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPP 398
           L  C +CL + +  K      C+  FC  C++ Y  + ++EG  S + CPD  C   L  
Sbjct: 28  LVTCKLCLCEQSLDKMTTLQECRCIFCTACLKQYMQLAIREGCGSPITCPDMVC---LNH 87

Query: 399 GLLKQ-----LLGAEEFERWESMLLTKTLESMSDVVYCP--RCETPCLEDVDHDAQ---- 458
           G L++     L+  ++F+ ++ +   + +       +CP   C+T C        Q    
Sbjct: 88  GTLQEAEIACLVPVDQFQLYQRLKFEREVHLDPCRTWCPVADCQTVCPVATSDPGQPVLV 147

Query: 459 -CSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQSSSQLSSDQRRKEREMINELMSV 518
            C  C+  FC+ C +  H  + C   +  +   E                    +  +  
Sbjct: 148 ECPSCHLKFCSCCKDAWHAEVSCRDSQPGILPTE--------------------HGTLFG 207

Query: 519 KEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDG---YDHFREGTC 560
            E     KQCP C++ I R EGC +M+C NC   FC+ C + +D      H+  G C
Sbjct: 208 TETDAPIKQCPVCRVYIERNEGCAQMMCKNCKHTFCWYCLQNLDNDIFLRHYDRGPC 241

[Close]

BLAST of Cp4.1LG15g01990 vs. NCBI nr
Match: XP_023554779.1 (E3 ubiquitin-protein ligase RNF14 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1229 bits (3181), Expect = 0.0
Identity = 605/607 (99.67%), Postives = 606/607 (99.84%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR
Sbjct: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD
Sbjct: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS
Sbjct: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN
Sbjct: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK
Sbjct: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT
Sbjct: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600

Query: 601 INAKVSS 607
           INAKV +
Sbjct: 601 INAKVGN 607

[Close]

BLAST of Cp4.1LG15g01990 vs. NCBI nr
Match: KAG7011377.1 (E3 ubiquitin-protein ligase RNF14 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1205 bits (3118), Expect = 0.0
Identity = 594/607 (97.86%), Postives = 598/607 (98.52%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M KTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGR+S YEHENRR
Sbjct: 1   MRKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRSSTYEHENRR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEI+DETAGNSGSSEATD
Sbjct: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIKDETAGNSGSSEATD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRLEMLQR SEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS
Sbjct: 121 DVYSRLEMLQRSSEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIE PGDITVTANLNSSGALEMKRTNSD+FSCSFKVKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIECPGDITVTANLNSSGALEMKRTNSDNFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISIQWLDAARISRLCSVLD IWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN
Sbjct: 241 YTISIQWLDAARISRLCSVLDAIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGH GDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK
Sbjct: 301 KGHSGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT
Sbjct: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREGTCELF QETIQQWEERMNARQLLGQI AELFPENGHPCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGTCELFPQETIQQWEERMNARQLLGQIHAELFPENGHPCPNCGQ 600

Query: 601 INAKVSS 607
           INAKV +
Sbjct: 601 INAKVGN 607

[Close]

BLAST of Cp4.1LG15g01990 vs. NCBI nr
Match: XP_022963554.1 (E3 ubiquitin-protein ligase RNF14 [Cucurbita moschata])

HSP 1 Score: 1199 bits (3103), Expect = 0.0
Identity = 591/607 (97.36%), Postives = 595/607 (98.02%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M KTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGR+S YEHENRR
Sbjct: 1   MRKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRSSTYEHENRR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEI+DETAGNSGSSEATD
Sbjct: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIKDETAGNSGSSEATD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRLEMLQR  EEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS
Sbjct: 121 DVYSRLEMLQRSFEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIE PGDITVTANLNSSGALEMKRTNSD+FSCSFKVKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIECPGDITVTANLNSSGALEMKRTNSDNFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISIQWLDA RIS LCSVLD IWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN
Sbjct: 241 YTISIQWLDATRISHLCSVLDAIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGH GDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK
Sbjct: 301 KGHSGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT
Sbjct: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREGTCELF QETIQQWEERMNARQLLGQI AELFPENGHPCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGTCELFPQETIQQWEERMNARQLLGQIHAELFPENGHPCPNCGQ 600

Query: 601 INAKVSS 607
           INAKV +
Sbjct: 601 INAKVGN 607

[Close]

BLAST of Cp4.1LG15g01990 vs. NCBI nr
Match: XP_022967205.1 (E3 ubiquitin-protein ligase RNF14 [Cucurbita maxima])

HSP 1 Score: 1196 bits (3093), Expect = 0.0
Identity = 589/607 (97.03%), Postives = 595/607 (98.02%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M KTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR
Sbjct: 1   MRKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRPRPVEKAEVSALADGIESFTCDEKPVEME+RCGDVVNSNCEI+D TAGNSGSSEATD
Sbjct: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEKRCGDVVNSNCEIKDGTAGNSGSSEATD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRL MLQR SEEPEL EEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS
Sbjct: 121 DVYSRLGMLQRSSEEPELSEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIE PGDITVTANLNSSGALEMKRTNSD+FSCSFKVKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIECPGDITVTANLNSSGALEMKRTNSDNFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQ SSLVYLE+DKEITLGPYN
Sbjct: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQMSSLVYLELDKEITLGPYN 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGH GDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQ AGTKFVRFPCK
Sbjct: 301 KGHSGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQLAGTKFVRFPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT
Sbjct: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCPRCETPCLED+DHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCPRCETPCLEDIDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREGTCELF QETIQQWEERMNARQLLGQI AELFPENGHPCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGTCELFPQETIQQWEERMNARQLLGQIHAELFPENGHPCPNCGQ 600

Query: 601 INAKVSS 607
           INAKV +
Sbjct: 601 INAKVGN 607

[Close]

BLAST of Cp4.1LG15g01990 vs. NCBI nr
Match: KAG6571648.1 (E3 ubiquitin-protein ligase RNF14, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1128 bits (2918), Expect = 0.0
Identity = 566/607 (93.25%), Postives = 571/607 (94.07%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M KTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGR+S YEHENRR
Sbjct: 1   MRKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRSSTYEHENRR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDV+NSNCEI+DETAGNSGSSEATD
Sbjct: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVLNSNCEIKDETAGNSGSSEATD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRLEMLQR SEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS
Sbjct: 121 DVYSRLEMLQRSSEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIE PGDITVTANLNSSGALEMKRTNSD+FSCSFKVKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIECPGDITVTANLNSSGALEMKRTNSDNFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISIQWLDAARISRLCSVLD IWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN
Sbjct: 241 YTISIQWLDAARISRLCSVLDAIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGH GDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK
Sbjct: 301 KGHSGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT
Sbjct: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCP                           SERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCP---------------------------SERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREGTCELF QETIQQWEERMNARQLLGQI AELFPENGHPCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGTCELFPQETIQQWEERMNARQLLGQIHAELFPENGHPCPNCGQ 580

Query: 601 INAKVSS 607
           INAKV +
Sbjct: 601 INAKVGN 580

[Close]

BLAST of Cp4.1LG15g01990 vs. ExPASy TrEMBL
Match: A0A6J1HKI4 (E3 ubiquitin-protein ligase RNF14 OS=Cucurbita moschata OX=3662 GN=LOC111463847 PE=3 SV=1)

HSP 1 Score: 1199 bits (3103), Expect = 0.0
Identity = 591/607 (97.36%), Postives = 595/607 (98.02%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M KTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGR+S YEHENRR
Sbjct: 1   MRKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRSSTYEHENRR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEI+DETAGNSGSSEATD
Sbjct: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIKDETAGNSGSSEATD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRLEMLQR  EEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS
Sbjct: 121 DVYSRLEMLQRSFEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIE PGDITVTANLNSSGALEMKRTNSD+FSCSFKVKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIECPGDITVTANLNSSGALEMKRTNSDNFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISIQWLDA RIS LCSVLD IWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN
Sbjct: 241 YTISIQWLDATRISHLCSVLDAIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGH GDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK
Sbjct: 301 KGHSGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT
Sbjct: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREGTCELF QETIQQWEERMNARQLLGQI AELFPENGHPCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGTCELFPQETIQQWEERMNARQLLGQIHAELFPENGHPCPNCGQ 600

Query: 601 INAKVSS 607
           INAKV +
Sbjct: 601 INAKVGN 607

[Close]

BLAST of Cp4.1LG15g01990 vs. ExPASy TrEMBL
Match: A0A6J1HTS2 (E3 ubiquitin-protein ligase RNF14 OS=Cucurbita maxima OX=3661 GN=LOC111466811 PE=3 SV=1)

HSP 1 Score: 1196 bits (3093), Expect = 0.0
Identity = 589/607 (97.03%), Postives = 595/607 (98.02%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M KTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR
Sbjct: 1   MRKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRPRPVEKAEVSALADGIESFTCDEKPVEME+RCGDVVNSNCEI+D TAGNSGSSEATD
Sbjct: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEKRCGDVVNSNCEIKDGTAGNSGSSEATD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRL MLQR SEEPEL EEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS
Sbjct: 121 DVYSRLGMLQRSSEEPELSEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIE PGDITVTANLNSSGALEMKRTNSD+FSCSFKVKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIECPGDITVTANLNSSGALEMKRTNSDNFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQ SSLVYLE+DKEITLGPYN
Sbjct: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQMSSLVYLELDKEITLGPYN 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGH GDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQ AGTKFVRFPCK
Sbjct: 301 KGHSGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQLAGTKFVRFPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT
Sbjct: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCPRCETPCLED+DHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCPRCETPCLEDIDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREGTCELF QETIQQWEERMNARQLLGQI AELFPENGHPCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGTCELFPQETIQQWEERMNARQLLGQIHAELFPENGHPCPNCGQ 600

Query: 601 INAKVSS 607
           INAKV +
Sbjct: 601 INAKVGN 607

[Close]

BLAST of Cp4.1LG15g01990 vs. ExPASy TrEMBL
Match: A0A5D3E5K0 (E3 ubiquitin-protein ligase RNF14 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold455G004660 PE=3 SV=1)

HSP 1 Score: 1030 bits (2664), Expect = 0.0
Identity = 502/606 (82.84%), Postives = 546/606 (90.10%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M K+GRGGR  D NR QEVWVAKH+N + N AS+S SQ     N P TGRN +YEH + R
Sbjct: 1   MRKSGRGGRWTDHNRNQEVWVAKHTNHSHNHASSSASQPSHLSNPPPTGRNFSYEHRHPR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRP+PV K EV++LADGIESFT DEKPV+      DV   NCE++ +T GNS S+EA D
Sbjct: 61  RPRPQPVHKPEVTSLADGIESFTFDEKPVDF-----DVAKPNCEVKQQTPGNSSSAEAAD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRLEMLQR SEEP L EEQL INDQLQEDELLALESIYGEN++ILDE++GQRRFQI 
Sbjct: 121 DVYSRLEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIF 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIE PGDIT+TA LNSS +LE+K  NSD+FSCSF VKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIENPGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISI+WLD+ARISRLCSVLD IW+EQ GQEVVYQWVE LQ SSL YL++DKEI LGPY+
Sbjct: 241 YTISIRWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYD 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGH GD RAVSGSVSPEVD+PAI+NYDNQRR+E+FCMNLQECCICLSQFAGTKFV+ PCK
Sbjct: 301 KGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYS+MHVKEGTVSKL CPDAKCD+M+PPGLLKQLLG EEFERWESM+LTKT
Sbjct: 361 HYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQ+SSQL S+QRRKEREMINEL+SVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREG+CELF QE IQQWEERMNARQ+LGQI+AELFPENGHPCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGHPCPNCGQ 600

Query: 601 INAKVS 606
           +NAKVS
Sbjct: 601 LNAKVS 601

[Close]

BLAST of Cp4.1LG15g01990 vs. ExPASy TrEMBL
Match: A0A1S3CQI9 (E3 ubiquitin-protein ligase RNF14 OS=Cucumis melo OX=3656 GN=LOC103503565 PE=3 SV=1)

HSP 1 Score: 1026 bits (2653), Expect = 0.0
Identity = 500/607 (82.37%), Postives = 545/607 (89.79%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M K+GRGGR  D NR QEVWVAKH+N + N AS+S SQ     N P TGRN +YEH + R
Sbjct: 1   MRKSGRGGRWTDHNRNQEVWVAKHTNHSHNHASSSASQPSHLSNPPPTGRNFSYEHRHPR 60

Query: 61  RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEATD 120
           RPRP+PV K EV++LADGIESFT DEKPV+      DV   NCE++ +T GNS S+EA D
Sbjct: 61  RPRPQPVHKPEVTSLADGIESFTFDEKPVDF-----DVAKPNCEVKQQTPGNSSSAEAAD 120

Query: 121 DVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQIS 180
           DVYSRLEMLQR SEEP L EEQL INDQLQEDELLALESIYGEN++ILDE++GQRRFQI 
Sbjct: 121 DVYSRLEMLQRSSEEPVLSEEQLSINDQLQEDELLALESIYGENVYILDEYKGQRRFQIF 180

Query: 181 IHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPY 240
           IHIE PGDIT+TA LNSS +LE+K  NSD+FSCSF VKYLNPIVLTCLLPKSYPSHLPPY
Sbjct: 181 IHIENPGDITITAKLNSSSSLEIKSPNSDEFSCSFNVKYLNPIVLTCLLPKSYPSHLPPY 240

Query: 241 YTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYN 300
           YTISI+WLD+ARISRLCSVLD IW+EQ GQEVVYQWVE LQ SSL YL++DKEI LGPY+
Sbjct: 241 YTISIRWLDSARISRLCSVLDGIWSEQVGQEVVYQWVEWLQMSSLAYLQLDKEIMLGPYD 300

Query: 301 KGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCK 360
           KGH GD RAVSGSVSPEVD+PAI+NYDNQRR+E+FCMNLQECCICLSQFAGTKFV+ PCK
Sbjct: 301 KGHSGDTRAVSGSVSPEVDIPAIINYDNQRRDEEFCMNLQECCICLSQFAGTKFVKLPCK 360

Query: 361 HYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKT 420
           HYFCWKCMETYS+MHVKEGTVSKL CPDAKCD+M+PPGLLKQLLG EEFERWESM+LTKT
Sbjct: 361 HYFCWKCMETYSSMHVKEGTVSKLNCPDAKCDVMVPPGLLKQLLGDEEFERWESMMLTKT 420

Query: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480
           LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE
Sbjct: 421 LESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEE 480

Query: 481 RQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540
           RQ+SSQL S+QRRKEREMINEL+SVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF
Sbjct: 481 RQNSSQLGSEQRRKEREMINELISVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYF 540

Query: 541 CYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNCGQ 600
           CYRCSKAIDGYDHFREG+CELF QE IQQWEERMNARQ+LGQI+AELFPENG PCPNCGQ
Sbjct: 541 CYRCSKAIDGYDHFREGSCELFPQEAIQQWEERMNARQVLGQIQAELFPENGRPCPNCGQ 600

Query: 601 INAKVSS 607
           +NAKV +
Sbjct: 601 LNAKVGN 602

[Close]

BLAST of Cp4.1LG15g01990 vs. ExPASy TrEMBL
Match: A0A6J1DZW6 (E3 ubiquitin-protein ligase RNF14 OS=Momordica charantia OX=3673 GN=LOC111025870 PE=3 SV=1)

HSP 1 Score: 982 bits (2538), Expect = 0.0
Identity = 491/609 (80.62%), Postives = 534/609 (87.68%), Query Frame = 0


Query: 1   MGKTGRGGRRADQNRTQEVWVAKHSNQTQNRASTSGSQRLEPGNRPSTGRNSNYEHENRR 60
           M KTGRGG R DQNRT   WVAKH NQT N +S+S  QR +  NRP T RNSNY   +RR
Sbjct: 1   MRKTGRGGWRDDQNRT---WVAKHGNQTHNHSSSSAPQRSDLRNRPYTSRNSNYASPSRR 60

Query: 61  --RPRPRPVEKAEVSALADGIESFTCDEKPVEMEERCGDVVNSNCEIRDETAGNSGSSEA 120
               R + V+KAE +ALAD +ES TCDEK             SN EI++E  GNS S EA
Sbjct: 61  PHASRSQFVQKAEANALADTVESLTCDEKHD---------TGSNSEIKEEGIGNSESGEA 120

Query: 121 TDDVYSRLEMLQRISEEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQ 180
            DDVY+RLEMLQ  S+EPEL EEQLRINDQLQEDELLALESIYGEN++ILDE RGQ  FQ
Sbjct: 121 RDDVYTRLEMLQLNSKEPELSEEQLRINDQLQEDELLALESIYGENVYILDEQRGQHCFQ 180

Query: 181 ISIHIEYPGDITVTANLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLP 240
           ISIHIE PG+I+VTA LNS GA+E+K  NSDDFSCSFKVKYL PIVLTCLLPKSYPSHLP
Sbjct: 181 ISIHIESPGEISVTAKLNSPGAVEIKSINSDDFSCSFKVKYLIPIVLTCLLPKSYPSHLP 240

Query: 241 PYYTISIQWLDAARISRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGP 300
           PYYTISIQWL++ARIS LCSVLD IWTEQ GQEVVYQWVE LQ SSL YL++DKEITLGP
Sbjct: 241 PYYTISIQWLESARISSLCSVLDGIWTEQVGQEVVYQWVEWLQMSSLAYLQLDKEITLGP 300

Query: 301 YNKGHLGDIRAVSGSVSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFP 360
           Y+K H GD+RAVSGSVSPEVDVPAI NYDNQ+R+EDFCMNLQECCICLSQ+AGTKFVRFP
Sbjct: 301 YDKRHSGDVRAVSGSVSPEVDVPAIKNYDNQKRDEDFCMNLQECCICLSQYAGTKFVRFP 360

Query: 361 CKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLT 420
           CKHYFCWKC+ETYS MHVKEGT+SKLKCPDAKCD+MLPPGLLKQLLG EEFERWESMLLT
Sbjct: 361 CKHYFCWKCLETYSRMHVKEGTISKLKCPDAKCDVMLPPGLLKQLLGDEEFERWESMLLT 420

Query: 421 KTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLL 480
           KTLESMSDVVYCPRCETPCLEDVDHDAQCSKC+FSFCTLC ERRHVGIECMTPEMKL LL
Sbjct: 421 KTLESMSDVVYCPRCETPCLEDVDHDAQCSKCFFSFCTLCRERRHVGIECMTPEMKLLLL 480

Query: 481 EERQSSSQLSSDQRRKEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQ 540
           +ERQ+SSQLS++QR+KEREMINEL+SVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQ
Sbjct: 481 QERQNSSQLSNNQRQKEREMINELLSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQ 540

Query: 541 YFCYRCSKAIDGYDHFREGTCELFSQETIQQWEERMNARQLLGQIRAELFPENGHPCPNC 600
           YFCYRCSKAIDGYDHFREGTCELF QETIQQWEERMN RQ++GQI+AELFPE+GHPCPNC
Sbjct: 541 YFCYRCSKAIDGYDHFREGTCELFPQETIQQWEERMNIRQVMGQIQAELFPEHGHPCPNC 597

Query: 601 GQINAKVSS 607
           GQ+NAKV +
Sbjct: 601 GQLNAKVGN 597

[Close]

BLAST of Cp4.1LG15g01990 vs. TAIR 10
Match: AT1G32340.1 (NDR1/HIN1-like 8 )

HSP 1 Score: 630.6 bits (1625), Expect = 1.3e-180
Identity = 318/600 (53.00%), Postives = 411/600 (68.50%), Query Frame = 0


Query: 38  QRLEPGNRPSTGRNSNYEHENRR----RPRPRPVEKAEVSALADGIESFTCDE------- 97
           Q L+   +PS        H NRR      +PRPVEK EV+     +     DE       
Sbjct: 59  QNLDHSTKPSK------SHRNRRSRGSNSKPRPVEKPEVNFAESDVVDCLADELSRLKVK 118

Query: 98  ---KPVEMEERC--GDVVNSNCE-------IRDETAGNSGSSEATDDV-YSRLEMLQRIS 157
                V MEE+       +SNCE         +E A      E  +D+  + L+ L+   
Sbjct: 119 QNSHSVNMEEKFHHSSSDHSNCEESELKRAESEEIADGVDEYETKEDIMLTILKDLRSSV 178

Query: 158 EEPELPEEQLRINDQLQEDELLALESIYGENIHILDEHRGQRRFQISIHIEYPGDITVTA 217
            EPEL EEQL++NDQLQEDELLAL  IYG N+ I D +   R FQ+ +++E   + T++ 
Sbjct: 179 SEPELTEEQLKMNDQLQEDELLALGYIYGGNMFIFDRYNDMRYFQVHVNVEATSEYTIST 238

Query: 218 NLNSSGALEMKRTNSDDFSCSFKVKYLNPIVLTCLLPKSYPSHLPPYYTISIQWLDAARI 277
            LN           S+DF  SFK ++L PIVL CLLPK+YPSHLPPY+ IS+QW++  +I
Sbjct: 239 KLNLQAD---SSKESEDFLYSFKAQHLPPIVLKCLLPKAYPSHLPPYFLISVQWMNPDKI 298

Query: 278 SRLCSVLDTIWTEQAGQEVVYQWVECLQTSSLVYLEIDKEITLGPYNKGHLGDIRAVSGS 337
           S LCS LD++W+EQ GQEV+YQW + LQ SS+ +L  D EI LGPY      D RAVSGS
Sbjct: 299 SSLCSKLDSLWSEQPGQEVLYQWTDWLQNSSISHLGFDNEIVLGPYGVTCSRDKRAVSGS 358

Query: 338 VSPEVDVPAIVNYDNQRREEDFCMNLQECCICLSQFAGTKFVRFPCKHYFCWKCMETYSN 397
            SP+ D+P I +YD+++R + F  +L ECCIC S+ AG  FV+ PC+H+FC KCM+TY++
Sbjct: 359 RSPDSDIPYIRSYDDEKRHDSFLQSLHECCICFSESAGIDFVKLPCQHFFCLKCMKTYTD 418

Query: 398 MHVKEGTVSKLKCPDAKCDLMLPPGLLKQLLGAEEFERWESMLLTKTLESMSDVVYCPRC 457
           +HV EGTV+KLKCPD+KC   +PPG+LK+LLG E +ERWE+++L KTLESM+DV YCPRC
Sbjct: 419 IHVTEGTVNKLKCPDSKCGETVPPGILKRLLGDEAYERWETLMLQKTLESMTDVAYCPRC 478

Query: 458 ETPCLEDVDHDAQCSKCYFSFCTLCSERRHVGIECMTPEMKLRLLEERQSSSQLSSDQRR 517
           ETPC+ED +  A C KCYFSFCTLC E+RHVG+ CM+PE++L++L+ERQ SS+L  +QRR
Sbjct: 479 ETPCIEDEEQLALCFKCYFSFCTLCKEKRHVGVACMSPELRLQILQERQDSSRLGEEQRR 538

Query: 518 KEREMINELMSVKEILRDAKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDH 577
           KE+EMINE+MSVK I++ AKQCPSCK+AISRT GCNKMVCNNCGQYFCYRC++AI GY+H
Sbjct: 539 KEKEMINEIMSVKVIMKSAKQCPSCKIAISRTGGCNKMVCNNCGQYFCYRCNEAITGYEH 598

Query: 578 FREGTCELFSQETIQQWEERMNARQLLGQIRAEL------FPENGHPCPNCGQINAKVSS 608
           F EG CELF QE IQ+W E MN RQ++GQI+A+L      FP+ G  CPNC Q N K  +
Sbjct: 599 FSEGKCELFPQEAIQEWNEMMNERQVIGQIQAQLFAQGGQFPQRGQLCPNCRQFNGKAGN 649

[Close]

BLAST of Cp4.1LG15g01990 vs. TAIR 10
Match: AT3G14250.1 (RING/U-box superfamily protein )

HSP 1 Score: 96.3 bits (238), Expect = 9.1e-20
Identity = 60/212 (28.30%), Postives = 94/212 (44.34%), Query Frame = 0


Query: 342 CCICLSQFAGTKFVR--FPCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGL 401
           C IC+ +   +   R    C H +C  C   Y    +KE   S++KCPD +C  ++ P  
Sbjct: 95  CMICMDEKPSSDIFRGTTNCTHAYCTDCTVRYVATKIKE-NASRIKCPDVECTRLIEPYT 154

Query: 402 LKQLLGAEEFERWESMLLTKTLESMSDVVYCP--RCETPCLEDVDHDA-----QCSKCYF 461
            + L+  + F+RWE  +L ++L S  D  YCP   C    + + + DA     +C  C+ 
Sbjct: 155 CRDLIPKDVFDRWEK-ILCESLISSWDKFYCPFKDCSAMMVNNENGDANVTQTECRSCHR 214

Query: 462 SFCTLCSERRHVGIECMTPEMKLRLLEERQSSSQLSSDQRRKEREMINELMSVKEILRDA 521
            FC  C    H GI C                 Q   + ++K  +  + L+      +  
Sbjct: 215 LFCVQCKVTWHAGIGC--------------DEFQRFGNTKKKSSDEDDALLIQMAKNKQW 274

Query: 522 KQCPSCKMAISRTEGCNKMVCNNCGQYFCYRC 545
           ++CPSCK  + + EGC  + C  CG  FCY C
Sbjct: 275 RRCPSCKFYVDKVEGCQHIKC-RCGYQFCYGC 289

[Close]

BLAST of Cp4.1LG15g01990 vs. TAIR 10
Match: AT3G53690.1 (RING/U-box superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.8e-15
Identity = 58/221 (26.24%), Postives = 102/221 (46.15%), Query Frame = 0


Query: 341 ECCICLSQFAGTKFVRF-PCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGL 400
           +C IC+   +  +  R   C H++C  C+  Y    +++  +S ++CP + C   L P  
Sbjct: 114 DCEICVDSKSIIESFRIGGCSHFYCNDCVSKYIAAKLQDNILS-IECPVSGCSGRLEPDQ 173

Query: 401 LKQLLGAEEFERWESMLLTKTLESMSDVVYCPRCETPCLEDVDH------DAQCSKCYFS 460
            +Q+L  E F+RW    L + +   S   YCP  +   L  ++       D++C  C+  
Sbjct: 174 CRQILPKEVFDRWGD-ALCEAVVMRSKKFYCPYKDCSALVFLEESEVKMKDSECPHCHRM 233

Query: 461 FCTLCSERRHVGIECMTPEMKLRLLEERQSSSQLSSDQRRKEREMINELMSVKEILRDAK 520
            C  C  + H  + C          EE Q   +L++++R ++  ++  +   K+     K
Sbjct: 234 VCVECGTQWHPEMTC----------EEFQ---KLAANERGRDDILLATMAKQKK----WK 293

Query: 521 QCPSCKMAISRTEGCNKMVCNNCGQYFCYRCSKAIDGYDHF 555
           +CPSCK  I +++GC  M C  CG  FCY C      + H+
Sbjct: 294 RCPSCKFYIEKSQGCLYMKC-RCGLAFCYNCGTPSRDHTHY 314

[Close]

BLAST of Cp4.1LG15g01990 vs. TAIR 10
Match: AT3G45470.1 (IBR domain containing protein )

HSP 1 Score: 71.2 bits (173), Expect = 3.1e-12
Identity = 55/211 (26.07%), Postives = 92/211 (43.60%), Query Frame = 0


Query: 337 MNLQECCICLSQF-AGTKFVRFPCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLML 396
           M  + C ICL +  A   FV   C H  C+ C+     + ++ GTV    C D +C L L
Sbjct: 1   MKGETCVICLEETKADRMFVMDKCLHRHCYPCVNQLVEVKLRNGTVP--TCLDYECKLKL 60

Query: 397 PPGLLKQLLGAEEFERWESMLLTKTLESMSDVVYCPRCETPCLEDVDHDAQCSKCYFSFC 456
                 ++L  +  E W+ M+  +++  ++  +YCP      L      ++ +K     C
Sbjct: 61  SLENCFKVLKPKVIELWKHMMKEESI-PLAKRIYCPYINCSTLMSKTEISRSNKSNDRAC 120

Query: 457 TLCSERRHVGIECMTPEMKLRLLEERQSSSQLSSDQRRKEREMINELMSVKEILRDAK-- 516
             CS    V I+C  P            S    ++ ++   + + + +++K +  D K  
Sbjct: 121 IKCSGL--VCIDCKVP----------WHSDLSCAEYKKLHPDPVLDDLTLKLLANDQKWR 180

Query: 517 QCPSCKMAISRTEGCNKMVCNNCGQYFCYRC 545
           QC  C+  I   +GCN M C  CG  FCY+C
Sbjct: 181 QCVKCRHLIELNQGCNHMTC-RCGYQFCYKC 195

[Close]

BLAST of Cp4.1LG15g01990 vs. TAIR 10
Match: AT4G01020.1 (helicase domain-containing protein / IBR domain-containing protein / zinc finger protein-related )

HSP 1 Score: 62.8 bits (151), Expect = 1.1e-09
Identity = 55/228 (24.12%), Postives = 91/228 (39.91%), Query Frame = 0


Query: 341  ECCICLSQFAGTKFVRFPCKHYFCWKCMETYSNMHVKEGTVSKLKCPDAKCDLMLPPGLL 400
            EC ICLS+     +    C H FC  C+       ++      + C    C   +    +
Sbjct: 1560 ECPICLSE-VDDGYSLEGCSHLFCKACLLEQFEASMRNFDAFPILCSHIDCGAPIVVADM 1619

Query: 401  KQLLGAEEFERWESMLLTKTLESMSDVV-YCPRCETPCLEDVDHDAQ------CSKCYFS 460
            + LL  E+ +   S  L+  + S    + +C   + P +  V    +      C  C+  
Sbjct: 1620 RALLSQEKLDELISASLSAFVTSSDGKLRFCSTPDCPSIYRVAGPQESGEPFICGACHSE 1679

Query: 461  FCTLCSERRHVGIECMTPEMKLRLLEERQSSSQLSSDQRRKEREMINELMSVKEIL--RD 520
             CT C    H  I C                     ++ +K +E  N  +S+K+    +D
Sbjct: 1680 TCTRCHLEYHPLITC---------------------ERYKKFKE--NPDLSLKDWAKGKD 1739

Query: 521  AKQCPSCKMAISRTEGCNKMVCNNCGQYFCYRC----SKAIDGYDHFR 556
             K+CP CK  I +T+GCN + C  CG++ C+ C    ++A   Y H R
Sbjct: 1740 VKECPICKSTIEKTDGCNHLQC-RCGKHICWTCLDVFTQAEPCYAHLR 1762

The following BLAST results are available for this feature:

BLAST of Cp4.1LG15g01990 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9JI90	1.7e-55	31.32	E3 ubiquitin-protein ligase RNF14 OS=Mus musculus OX=10090 GN=Rnf14 PE=1 SV=2	[more]
Q9UBS8	2.9e-55	30.87	E3 ubiquitin-protein ligase RNF14 OS=Homo sapiens OX=9606 GN=RNF14 PE=1 SV=1	[more]
Q04638	1.3e-23	28.09	Translation termination inhibitor protein ITT1 OS=Saccharomyces cerevisiae (stra...	[more]
Q54CX4	1.4e-17	26.32	Uncharacterized protein DDB_G0292642 OS=Dictyostelium discoideum OX=44689 GN=DDB...	[more]
A5PK27	1.0e-15	24.47	E3 ubiquitin-protein ligase RNF144B OS=Bos taurus OX=9913 GN=RNF144B PE=2 SV=1	[more]

BLAST of Cp4.1LG15g01990 vs. NCBI nr


Match Name	Identity	Description
XP_023554779.1	99.67	E3 ubiquitin-protein ligase RNF14 [Cucurbita pepo subsp. pepo]	[more]
KAG7011377.1	97.86	E3 ubiquitin-protein ligase RNF14 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022963554.1	97.36	E3 ubiquitin-protein ligase RNF14 [Cucurbita moschata]	[more]
XP_022967205.1	97.03	E3 ubiquitin-protein ligase RNF14 [Cucurbita maxima]	[more]
KAG6571648.1	93.25	E3 ubiquitin-protein ligase RNF14, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Cp4.1LG15g01990 vs. ExPASy TrEMBL


Match Name	Identity	Description
A0A6J1HKI4	97.36	E3 ubiquitin-protein ligase RNF14 OS=Cucurbita moschata OX=3662 GN=LOC111463847 ...	[more]
A0A6J1HTS2	97.03	E3 ubiquitin-protein ligase RNF14 OS=Cucurbita maxima OX=3661 GN=LOC111466811 PE...	[more]
A0A5D3E5K0	82.84	E3 ubiquitin-protein ligase RNF14 OS=Cucumis melo var. makuwa OX=1194695 GN=E567...	[more]
A0A1S3CQI9	82.37	E3 ubiquitin-protein ligase RNF14 OS=Cucumis melo OX=3656 GN=LOC103503565 PE=3 S...	[more]
A0A6J1DZW6	80.62	E3 ubiquitin-protein ligase RNF14 OS=Momordica charantia OX=3673 GN=LOC111025870...	[more]

BLAST of Cp4.1LG15g01990 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G32340.1	1.3e-180	53.00	NDR1/HIN1-like 8	[more]
AT3G14250.1	9.1e-20	28.30	RING/U-box superfamily protein	[more]
AT3G53690.1	1.8e-15	26.24	RING/U-box superfamily protein	[more]
AT3G45470.1	3.1e-12	26.07	IBR domain containing protein	[more]
AT4G01020.1	1.1e-09	24.12	helicase domain-containing protein / IBR domain-containing protein / zinc finger...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 410..468 e-value: 4.2E-11 score: 52.9 coord: 497..559 e-value: 8.1E-5 score: 32.0
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 509..548 e-value: 7.4E-7 score: 29.3 coord: 410..468 e-value: 2.4E-9 score: 37.3
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 515..598 e-value: 1.4 score: 10.0 coord: 342..389 e-value: 4.4E-4 score: 29.6
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 342..390 score: 10.350093
IPR006575	RWD domain	SMART	SM00591	RWD2001b	coord: 152..287 e-value: 1.5E-21 score: 87.6
IPR006575	RWD domain	PFAM	PF05773	RWD	coord: 148..283 e-value: 3.9E-24 score: 85.2
IPR006575	RWD domain	PROSITE	PS50908	RWD	coord: 152..287 score: 15.953697
None	No IPR available	GENE3D	1.20.120.1750		coord: 483..587 e-value: 2.1E-15 score: 59.3
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 52..69
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 18..51
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..17
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..69
None	No IPR available	PANTHER	PTHR11685:SF297	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 128..587
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 501..561
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 410..472
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 339..423
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 338..425 e-value: 1.1E-18 score: 68.9
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	GENE3D	3.10.110.10	Ubiquitin Conjugating Enzyme	coord: 140..289 e-value: 1.3E-27 score: 97.9
IPR016135	Ubiquitin-conjugating enzyme/RWD-like	SUPERFAMILY	54495	UBC-like	coord: 146..282
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 128..587
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 359..368
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 338..564 score: 25.502958

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG15g01990.1	Cp4.1LG15g01990.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0005515	protein binding
molecular_function	GO:0004842	ubiquitin-protein transferase activity