Cp4.1LG14g04630 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG14g04630
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	trypsin inhibitor 1-like
Location	Cp4.1LG14: 1540040 .. 1540279 (+)
RNA-Seq Expression	Cp4.1LG14g04630
Synteny	Cp4.1LG14g04630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGACTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGCTGCCCTTGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCGTATGCCGGCCCAACGGCTTCTGTGGTTGA

mRNA sequence

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGACTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGCTGCCCTTGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCGTATGCCGGCCCAACGGCTTCTGTGGTTGA

Coding sequence (CDS)

ATGGAGTGGAAAAGAGGTGCTTTAATTGCCATGGTGGGGCTGTTGCTGATGGCGGCTTTTGCAGACTCCGCCGCCGTCCATGGCGGCGAAGTGATTCAGCTAGTTTCCGACGGAGTGAATGATTTCCCAAGGAAGATGATGAACGCTGCCCTTGGTTGCCCTAGAATCCTTATGGAGTGCGAAGTGGATTCCGACTGCCTTCCTGGTTGCGTATGCCGGCCCAACGGCTTCTGTGGTTGA

Protein sequence

MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMECEVDSDCLPGCVCRPNGFCG

Homology

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy Swiss-Prot
Match: Q43667 (Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 8.6e-16
Identity = 39/65 (60.00%), Postives = 46/65 (70.77%), Query Frame = 0


Query: 17 MAAFADSAAVHGG--EVIQLVSDGVNDFPRKMMNAALGCPRILMECEVDSDCLPGCVCRP 76
          MAAF +SA    G  EVIQLVSDGVN++  KMM   + CPRILM C+V+ DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVACPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 65

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy Swiss-Prot
Match: P34950 (Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 7.5e-12
Identity = 35/65 (53.85%), Postives = 45/65 (69.23%), Query Frame = 0


Query: 17 MAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALG--CPRILMECEVDSDCLPGCVCRP 76
          MA+ A+S+ V   EVI+L+SDG ND PRK+M+   G  CPRILM C+ D DC+  C C  
Sbjct: 1  MASVAESSGV--VEVIELISDGGNDLPRKIMSGRHGGICPRILMPCKTDDDCMLDCRCLS 60

Query: 77 NGFCG 80
          NG+CG
Sbjct: 61 NGYCG 63

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy Swiss-Prot
Match: P12071 (Trypsin inhibitor 2 OS=Ecballium elaterium OX=3679 PE=1 SV=2)

HSP 1 Score: 59.3 bits (142), Expect = 2.3e-08
Identity = 23/28 (82.14%), Postives = 24/28 (85.71%), Query Frame = 0


Query: 52 GCPRILMECEVDSDCLPGCVCRPNGFCG 80
          GCPRILM C+ DSDCL GCVC PNGFCG
Sbjct: 1  GCPRILMRCKQDSDCLAGCVCGPNGFCG 28

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy Swiss-Prot
Match: P11968 (Trypsin inhibitor 2 OS=Bryonia dioica OX=3652 PE=1 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 2.5e-07
Identity = 21/28 (75.00%), Postives = 24/28 (85.71%), Query Frame = 0


Query: 52 GCPRILMECEVDSDCLPGCVCRPNGFCG 80
          GCPRILM C+ DSDCL GCVC+ NG+CG
Sbjct: 2  GCPRILMRCKRDSDCLAGCVCQKNGYCG 29

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy Swiss-Prot
Match: P84452 (Trypsin inhibitor 5 OS=Sechium edule OX=184140 PE=1 SV=1)

HSP 1 Score: 55.1 bits (131), Expect = 4.3e-07
Identity = 19/27 (70.37%), Postives = 24/27 (88.89%), Query Frame = 0


Query: 53 CPRILMECEVDSDCLPGCVCRPNGFCG 80
          CPRILM+C++D+DC P C CRP+GFCG
Sbjct: 1  CPRILMKCKLDTDCFPTCTCRPSGFCG 27

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: XP_023552845.1 (uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 166 bits (421), Expect = 8.13e-51
Identity = 79/79 (100.00%), Postives = 79/79 (100.00%), Query Frame = 0


Query: 1   MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMEC 60
           MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMEC
Sbjct: 85  MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMEC 144

Query: 61  EVDSDCLPGCVCRPNGFCG 79
           EVDSDCLPGCVCRPNGFCG
Sbjct: 145 EVDSDCLPGCVCRPNGFCG 163

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: KAG6576915.1 (hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 163 bits (412), Expect = 1.79e-49
Identity = 76/79 (96.20%), Postives = 78/79 (98.73%), Query Frame = 0


Query: 1   MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMEC 60
           MEWKRGALIAMVGLLLMAAFA+SAAVHGGEVIQLVSDGVNDFPRKMMN ALGCPRILMEC
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGALGCPRILMEC 142

Query: 61  EVDSDCLPGCVCRPNGFCG 79
           EVDSDCLPGC+CRPNGFCG
Sbjct: 143 EVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: KAG7014942.1 (hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 163 bits (412), Expect = 1.79e-49
Identity = 76/79 (96.20%), Postives = 78/79 (98.73%), Query Frame = 0


Query: 1   MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMEC 60
           MEWKRGALIAMVGLLLMAAFA+SAAVHGGEVIQLVSDGVNDFPRKMMN ALGCPRILMEC
Sbjct: 83  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGALGCPRILMEC 142

Query: 61  EVDSDCLPGCVCRPNGFCG 79
           EVDSDCLPGC+CRPNGFCG
Sbjct: 143 EVDSDCLPGCICRPNGFCG 161

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: XP_022984819.1 (trypsin inhibitor 1-like [Cucurbita maxima])

HSP 1 Score: 158 bits (399), Expect = 1.36e-48
Identity = 73/79 (92.41%), Postives = 76/79 (96.20%), Query Frame = 0


Query: 1  MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMEC 60
          MEWKRGALIAMVGLLLMAAFA+SAAVHGGEVIQLVSDGVNDFPRKMMN   GCPRILMEC
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQGCPRILMEC 60

Query: 61 EVDSDCLPGCVCRPNGFCG 79
          E+DSDCLPGC+CRPNGFCG
Sbjct: 61 ELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Cp4.1LG14g04630 vs. NCBI nr
Match: KAG6576914.1 (hypothetical protein SDJN03_24488, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 107 bits (266), Expect = 1.51e-27
Identity = 55/81 (67.90%), Postives = 60/81 (74.07%), Query Frame = 0


Query: 1  MEWKRGALIAMVGLLLMAAFADSAAVHGG--EVIQLVSDGVNDFPRKMMNAALGCPRILM 60
          MEWKRGAL+AMVGLLL+AAFA+S AV     EVIQLVSDG NDFPRKMM  A  CPR L+
Sbjct: 1  MEWKRGALVAMVGLLLIAAFAESTAVDAAVDEVIQLVSDGGNDFPRKMMKGAEECPRQLV 60

Query: 61 ECEVDSDCLPGCVCRPNGFCG 79
           C+ DSDC   C C P  FCG
Sbjct: 61 RCKKDSDCHRACKCMPYHFCG 81

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy TrEMBL
Match: A0A6J1JBL8 (trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1)

HSP 1 Score: 158 bits (399), Expect = 6.56e-49
Identity = 73/79 (92.41%), Postives = 76/79 (96.20%), Query Frame = 0


Query: 1  MEWKRGALIAMVGLLLMAAFADSAAVHGGEVIQLVSDGVNDFPRKMMNAALGCPRILMEC 60
          MEWKRGALIAMVGLLLMAAFA+SAAVHGGEVIQLVSDGVNDFPRKMMN   GCPRILMEC
Sbjct: 1  MEWKRGALIAMVGLLLMAAFAESAAVHGGEVIQLVSDGVNDFPRKMMNGVQGCPRILMEC 60

Query: 61 EVDSDCLPGCVCRPNGFCG 79
          E+DSDCLPGC+CRPNGFCG
Sbjct: 61 ELDSDCLPGCICRPNGFCG 79

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy TrEMBL
Match: A0A0A0KY34 (Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 3.06e-25
Identity = 46/81 (56.79%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 1  MEWKRGALIAMVGLLLMAAFADSAAVH-GGEVIQLVSDGVNDFPRKM-MNAALGCPRILM 60
          MEWK+ AL+AM+G+LLMA F +S  +    E+IQLVSDGVN++ + +  + A GCPRILM
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGLGVDEEIIQLVSDGVNEYSQNIGKDTAPGCPRILM 60

Query: 61 ECEVDSDCLPGCVCRPNGFCG 79
          +C+ DSDC PGC C+P G+CG
Sbjct: 61 KCKTDSDCYPGCTCKPMGYCG 81

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy TrEMBL
Match: A0A5D3E0W0 (Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G00600 PE=3 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 4.12e-22
Identity = 44/80 (55.00%), Postives = 57/80 (71.25%), Query Frame = 0


Query: 1  MEWKRGALIAMVGLLLMAAFADSAAVH-GGEVIQLVSDGVNDFPRKMMNAALGCPRILME 60
          MEWK+ AL+AM+G+LLMA F +S       E+IQLVSDGVN++     +   GCPRILM+
Sbjct: 1  MEWKKIALVAMMGMLLMATFTESVGFGVDEEIIQLVSDGVNEY-----SGHPGCPRILMK 60

Query: 61 CEVDSDCLPGCVCRPNGFCG 79
          C+ D DCL GC C+ NG+CG
Sbjct: 61 CKTDRDCLAGCTCKRNGYCG 75

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy TrEMBL
Match: A0A4P2SJP1 (Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 85.5 bits (210), Expect = 3.03e-20
Identity = 40/65 (61.54%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 17 MAAFADSAAVHGG--EVIQLVSDGVNDFPRKMMNAALGCPRILMECEVDSDCLPGCVCRP 76
          MAAF +SA    G  EVIQLVSDGVN++  KMM   +GCPRILM C+V+ DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMKGVVGCPRILMPCKVNDDCLRGCKCLS 60

Query: 77 NGFCG 79
          NG+CG
Sbjct: 61 NGYCG 65

[Close]

BLAST of Cp4.1LG14g04630 vs. ExPASy TrEMBL
Match: A0A4P2SLY2 (Trypsin inhibitor I2 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1)

HSP 1 Score: 85.1 bits (209), Expect = 4.30e-20
Identity = 39/65 (60.00%), Postives = 47/65 (72.31%), Query Frame = 0


Query: 17 MAAFADSAAVHGG--EVIQLVSDGVNDFPRKMMNAALGCPRILMECEVDSDCLPGCVCRP 76
          MAAF +SA    G  EVIQLVSDGVN++  KMM   +GCPR+LM C+V+ DCL GC C  
Sbjct: 1  MAAFVESARAGAGADEVIQLVSDGVNEYSEKMMEGVVGCPRVLMPCKVNDDCLRGCKCLS 60

Query: 77 NGFCG 79
          NG+CG
Sbjct: 61 NGYCG 65

The following BLAST results are available for this feature:

BLAST of Cp4.1LG14g04630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q43667	8.6e-16	60.00	Trypsin inhibitor 1 OS=Trichosanthes kirilowii OX=3677 PE=3 SV=1	[more]
P34950	7.5e-12	53.85	Trypsin inhibitor 5 OS=Luffa aegyptiaca OX=3670 PE=1 SV=1	[more]
P12071	2.3e-08	82.14	Trypsin inhibitor 2 OS=Ecballium elaterium OX=3679 PE=1 SV=2	[more]
P11968	2.5e-07	75.00	Trypsin inhibitor 2 OS=Bryonia dioica OX=3652 PE=1 SV=1	[more]
P84452	4.3e-07	70.37	Trypsin inhibitor 5 OS=Sechium edule OX=184140 PE=1 SV=1	[more]

BLAST of Cp4.1LG14g04630 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023552845.1	8.13e-51	100.00	uncharacterized protein LOC111810370 [Cucurbita pepo subsp. pepo]	[more]
KAG6576915.1	1.79e-49	96.20	hypothetical protein SDJN03_24489, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG7014942.1	1.79e-49	96.20	hypothetical protein SDJN02_22573, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_022984819.1	1.36e-48	92.41	trypsin inhibitor 1-like [Cucurbita maxima]	[more]
KAG6576914.1	1.51e-27	67.90	hypothetical protein SDJN03_24488, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Cp4.1LG14g04630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JBL8	6.56e-49	92.41	trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111482991 PE=3 SV=1	[more]
A0A0A0KY34	3.06e-25	56.79	Trypsin inhibitor 1 OS=Cucumis sativus OX=3659 GN=Csa_4G000810 PE=3 SV=1	[more]
A0A5D3E0W0	4.12e-22	55.00	Trypsin inhibitor 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold655G...	[more]
A0A4P2SJP1	3.03e-20	61.54	Trypsin inhibitor I1 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]
A0A4P2SLY2	4.30e-20	60.00	Trypsin inhibitor I2 OS=Trichosanthes kirilowii OX=3677 PE=2 SV=1	[more]

BLAST of Cp4.1LG14g04630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	SMART	SM00286	PTI_1	coord: 51..79 e-value: 4.0E-9 score: 46.3
None	No IPR available	GENE3D	4.10.75.20		coord: 32..79 e-value: 3.7E-16 score: 60.7
IPR000737	Proteinase inhibitor I7, squash	PFAM	PF00299	Squash	coord: 53..79 e-value: 7.8E-14 score: 51.4
IPR000737	Proteinase inhibitor I7, squash	PROSITE	PS00286	SQUASH_INHIBITOR	coord: 53..72
IPR000737	Proteinase inhibitor I7, squash	CDD	cd00150	PlantTI	coord: 53..79 e-value: 1.97818E-11 score: 51.2243
IPR011052	Proteinase/amylase inhibitor domain superfamily	SUPERFAMILY	57027	Plant inhibitors of proteinases and amylases	coord: 52..79

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG14g04630.1	Cp4.1LG14g04630.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity
molecular_function	GO:0004866	endopeptidase inhibitor activity