Cp4.1LG10g10810 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g10810
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionNADH--cytochrome b5 reductase 1-like
LocationCp4.1LG10: 6608643 .. 6614364 (+)
RNA-Seq ExpressionCp4.1LG10g10810
SyntenyCp4.1LG10g10810
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: five_prime_UTRexonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCAACTCTAAAAGTTAATTTCTTCCCCATTGGTTGGTTATTCAAGAAAAATTGTGTATCACAGTCGATGAATTTAGCTCATCCATTTGTTTTTCATTAGTTTTTCCAAATACCTGCCTCAGGCTTCACCTAATTCGGCGTCACTTCATTTTTCTTCAGTGGTTGTGGTGGCTGTGGCTGGGGTTGGTGAAAGCGGGTGTCGCGGCGTGGGGCCGGAAAAAGTTAGGTGAATCGTAATCCATTCTAGAAATCCTTTAAATTCCACTCCAAATATCGAAAACCCATTTGATTTTTCTTGAGGTTTTTTTCTGGGAAGAATCGGTGATGGATCTCTCACAGTCTTTAGATCCGCAAGTCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCGTCTAAAAAAAGCAAATCCAGAGGTTAGCTACTCTTGAATCTCAGTTTTTCTGTAGTTCCCATTGCTTCGATTTCTGGGATCGGTTGTGTTTTGATTTGTTCTCTTTTATGAAAATTTGAGATTCCGGTCATGTCATTTTGTGAATAAGTGGAGTTTTTGAGTTGGAAATCGATATAGATTAAGGATTGGATCCATATCTGTTCTTATTTTGGATGGTGTTATATTATCTGGTTAATCTCTTTGTATCAACTCATAGTTTAAATCTTATTTGTGAAAATTACATATAAAGAGGAGATAAATTGAACTCACTACCCATTAAGTGGGTCAATGCTGTCTACTTGAGCTTGAACTATGTTCAAGTGAGCTAGCTTATAGTAACTTGAACTTTCTTGAAGTTGTAGAAGCTAATTAATCTATGTTACATGGTTCCATTTTCTGTCTAGATCTGTAATGGACATTCCAGTTCAAATTCGACCGATGATCTGTTTTAAATTGATATCAGTTAAGGAGATTTGATGGGAAACATCACATAAACAGGGCTTCATGTTAGAGTTGTCCATGAAAATATTGTATCGTTTGATAGTAAGAAGGGTCGATTCAGTAATGTGTGGACCGAATGTGGTGTTCACTCAACCCTTTAGCAGGATTCTAGTGCTTTTGGTGGTTTTGTTGTGATGATACATTAGAACATTGTCTGTTATTGGTGCTTTATTTGATTCGTTTACTTAATTTGTTTACTGTATGATTGGAAAGACGTGCTCGTATGTTCTTGGGTAAGCACGAAAGCATCCATACTTTTATTGAGGCTGCATCCTTTCTAGCTTTATCTTGGAGTGAATTACCTTCTCCATTTTGTACTTATCACAGTGGAGGTGTTTTTTGTGTTTGTAATTATCCTCTTATAGGACATTCTTTTGTAATCGATTTCATTGATTAATGAAGTGGTTTATGTTTCCTCAAAGTTTAATTTGTTTTCATTATGATATGGTTTTGTGCATTCAGGTTGCTTGGATCCAGAGGTATTCAAGGAGTTTAAACTTATCAAACGCGTGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCAAATCCTACTTCAGTTTTGGGTCTTCCCATTGGACAACATATCAGTTGCAGGTCTAGTAGTTGATTCTTATTTATTCTTCATAGGATTATTTGGATTTCTACTCGCCATGGCTATGGATACGTAGCTGATTGAAAAAACAATGTTGATTTTGACACTATATGGTGTAGGAATCACAATACTATACTCTCCTGTGTTAACTCGATAACACTTAGAGATAACCTGAAATAAGCAAGTTTCCTTTAAAGATACCTGTTAAACTGTAACGATCCTAGCCTACCGCTAGCAGATATTGTCCTCTTTGGGCTTTCTCTTAAGGTTTTTAAAACGCATCTGTTAGGGAGAGGTTTCCACACCCTTATAAAGGGTATTTCGTTCTCCTCCCCAACCGATGTGGGATCTCACAATCCACCCCCTTCGGGGTCCAGCGTCCTCGCTGGCACTCGTTCCCTTCTCCAATCGACGTAGGACCCCCCAATCCACCCTCCTTCGAAGCCCAACGTCTTTGTTGGCACACCGCCTCGTGTTCACTCCCCCTTTGGGGCTTAGCCTCTTTGCTGGCACATCGCCCGGTGTCTGGCTCTGATACCAACTGTAACAGCCCAAGCCCACCGTTAGCAGATATTGTCCTTTTTGGGCTTTCCCTTTCGGGCTTCCCTTCAAGGTTTTTAAAATGCGTCTGCGAGGGAGAAGTTTCCACGCTCTTATATAGAATGCTTCGTTCTCCTCCCCAACCGATGTGGGATCTCACATAAACGGTCAATCCTGCCTCTTCTTCATTATAGATTTTTTTGATTCAGCTCATAGCCGGTCACAACTTTTGTTGGATAAGGTCACTTGACATTATATTATTTAGGCTACGACTGGTCTTTTCGCCTGAGAGATATTGTAGGACTTTAAAGAAAACTTCTAAAACTTTGTTACGGTGGAGTATCTTGTCTCTTAGCTGCTTGATTGTGTTCATCGTTGAGAAACTTTTCTATTCTTTAAATATGCTAACCAGAAATGATTTCCGGACTCTCCCTTCGGTTTGAGTGGGGATGTGTGCCTTATTGCCCTCTTCACCTGGTGTTGTTTTCGAAGATGACTGTTCCATATTGGATCATTTACTGTCCCTGAGAAATTATAATTCATTCATCTTACAGGGGCAAGGATGGCCAAGGTGAAGAGGTCATCAAACCGTACACACCGATTACTTTAGATTCTGATGTTGGTTATTTTGAGCTCGTCATAAAGGTTTGTATTTTCTGATTCTTATTTGATGAGCAGTATTTTAATGGTATGCATACTTGTTAATCTGAACTCTGTAATGTGTTTAATCAGATGTATCCCCAAGGAAGGATGTCGCATCATTTCCGACAGATGCGTGTTGGCGATTTTCTTGCTGTAAAGGGGCCCAAGGTACGTTCAATCAATAATGACAGCTTTAAGTCATGTTTTTTATGCTTTAAGTATTGATGTTTTTTGGGATTCGAGCATTCAGCTTCCTTCAGGTTCGGCTGCAGACTTAATGAAACTTTTTTTTTTGAAGAATTTAATGAAACATTTTGTTTGTCATAGACACAAAGGCCCCTCTTATTCTTTCTCTAGTGAGAGAGAAACAAAGAACTTTATGGTACCTTTGAAAACACTGAGAATATTCAAAATTCACAACGAAAATATTTCTGCAGGGGCGTTTCAGGTATCAACCTGGGCAGGTCCGAGCATTTGGGATGCTCGCTGGAGGCTCCGGCATTACTCCTATGTATCAAGTACGAATTTCCTTTTCTTTTGGCTTGATCATTTTAGTTACTCAACTTACCATCATTCGGACGACTTGATTGTTGTTATCGATACTTCGCTTTCTTTTCTGCTCCTTAGGTTGCTCGAGCAATATTAGAAAATCCGAACGACGAGACGAAGGTACACCTCATCTATGCCAATGTCACCTATGAGGACATTCTTTTAAAGGTAATGTTGTCATCTTTCTTCAATAAACTTACCCCTCGTCCTCGTATTGACCATAAATTTGGATACCAGTTGATCCTCTCATTTCGAAACATGTTGATGGTTAGTTTTTCTCTTAATGGAGAGAGATAAACCAGGCTAACTGGTGTGACCGATACTCGAGTGGTATCGTAATCAATCTACTCCTTTCTCAGTGCTATGAATTTCAACTGCCGAGTCATTTGATGAAAATGTTTTCGGTACTTTCATGTTTGAACTCCTTAGCTGAACACGTGTATGGAGATCGTTTATCAACTTAAGTATAGTTTAATTAGTCAAGGCATATATCCTCGATCGTAAAGTCAGGGTTTGTCATGTGCGATTTTGGGTATTGAAGAGTGAAGGATCTTACTATTTTGGTAGCCTCTTCTTGGGCTTCCACTTTTGCTTTCATAATAATAATTCAATTTCTATGATTTGTGTATCTCTCTATAATTTCATCACTTTTCCTTACCAAAAGAAACTTGTGTGGTTGTAGGAGGAGTTGGATCTTCTTGCCAAAAGGTACCCAGATAGCTTCAAGCTCTACTATGTCTTAAACCAGGTCAGTCTTCTCCTTCACTCACAGCCCCAAATTTATCCTTTTCTCTGTGTTTAAACTTTTTACCATTACAAGATCTCGATGTCAAATGACGGGAAATCGTATTATGCTTATTTTGTTTTGTTGTCTAGACCGATATTAAGTGATCGAAATGAACTTCGACGAATCTAGTGAATTAAACTGATTGGTATACTATGTGATTAGATCTCTTTCTTCAAATCTGGCCGTTCTTAAAAGTTATCATAGCTCGATCTTGTTCTATTGGCTTGAAGTCCATTTTTTGTAGCAGGGTCGAAGATTCTTCGTAAAAGTTAAAACATAAATGAAATTACTTTTAGTCGACTGTTCTCGGAAAGTTGTTTTGTTCTTGTGAGTGCTCTAGAGTCCCCCGAGCTAGTGAACACTTTCGGATTCGTAAACTTGTGCATTGTGGATGGAATTGGGTTCTCTAAGGCCATGATGGATCTCCTTGGGAGCTTGATTTATGCTTGCTTGATTATGTACTTTGTTTAATGGTTTGCAATGTCATATCTTTTAATCAACCATTCATATATCAATTGCATACTCAGCCTCCTGAAGAATGGGATGGTGGTGTCGGATTCGTATCGAAGGAGATGATTCAAACCCACTGCCCCGCTCCTGCTTCTGATATTCAGGTATATTCGATTACGACTATGGTTTCAAATAATACGAATGCTTTGGATCTGTTTTCGAAACCATGTTTGAACGATTTCTTTTTGTCCGAAGTCTGTTCTCTTGTTTTCTTGTAATTTATTCTTACGAACGACCTGTTTCCAAAAGCGAACGAAGATGTTCTTTCATGTGCACAATAAGTTGGATCGGAGTAGGACCGTTGGATCATTCACTTGTCCGTGTTAATAGGTTTAAAAATTAAGCTTATAAACACTACCTTCACCATTCGTACGCATTTACTCATTGGATCATTCGCTGCGCATTTATGAGATTAATAGGATTGTTTGCTGCTGTCTGATCTTTTATTCATAAAACAACCAAATTTTATGTGTTATACTGTTTTGTAAGCAGGTTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGGTGCTCATCTTGAAGCTCTTGGATATGCACCTGAGATGCTGTTCATGTTCTGAGCCTCCCTCAACCTCCACACTAGAGCTAAGCATCATTTATTTTTCTTCCCCCTTCTAAATTTTGAACATTATGCACTGAGAGGATGTTGCCTTAGCTCTTTTCACTTTGCAATGTTGTTGAACTTTGATGCCATCAATAACTTCAACTCTTAGAGCTAGTGAGAGTCTAGACTAGTTTTCATTCACATTGTTTGTTCATGTAACAATTCTAGAATGGTTTTCTTCTCGAAGTGTAATAAGACCGTTTAGATTAGCTTTTTAAGTGCTTGAAAACGCTTTTCTTTGCATCTAAAAGTCAAAGCCCAAGTCTACTAGTGGCAGAGATTGTCCGCTTTGGCTCATTTTGGCCCATTACGTACTGTCAGCTTCACGGGTTTTAAAATGTGTCTATTAGGGAGAGTTTTTCAAACTCTTATAAAGAATTATTTGTTTCCCTCTCCAGCCGATGTGAGATCTTATAATCCACCCTCCTTCAAGGCCCAGCATTCTCGTTGGCACACTGCTTAGTGTCTGGCTTTGATACCATTTGTAAGTCTACCGCTAGCAG

mRNA sequence

TCCAACTCTAAAAGTTAATTTCTTCCCCATTGGTTGGTTATTCAAGAAAAATTGTGTATCACAGTCGATGAATTTAGCTCATCCATTTGTTTTTCATTAGTTTTTCCAAATACCTGCCTCAGGCTTCACCTAATTCGGCGTCACTTCATTTTTCTTCAGTGGTTGTGGTGGCTGTGGCTGGGGTTGGTGAAAGCGGGTGTCGCGGCGTGGGGCCGGAAAAAGTTAGGTGAATCGTAATCCATTCTAGAAATCCTTTAAATTCCACTCCAAATATCGAAAACCCATTTGATTTTTCTTGAGGTTTTTTTCTGGGAAGAATCGGTGATGGATCTCTCACAGTCTTTAGATCCGCAAGTCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCGTCTAAAAAAAGCAAATCCAGAGAGGTATTCAAGGAGTTTAAACTTATCAAACGCGTGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCAAATCCTACTTCAGTTTTGGGTCTTCCCATTGGACAACATATCAGTTGCAGGGGCAAGGATGGCCAAGGTGAAGAGGTCATCAAACCGTACACACCGATTACTTTAGATTCTGATGTTGGTTATTTTGAGCTCGTCATAAAGATGTATCCCCAAGGAAGGATGTCGCATCATTTCCGACAGATGCGTGTTGGCGATTTTCTTGCTGTAAAGGGGCCCAAGGGGCGTTTCAGGTATCAACCTGGGCAGGTCCGAGCATTTGGGATGCTCGCTGGAGGCTCCGGCATTACTCCTATGTATCAAGTTGCTCGAGCAATATTAGAAAATCCGAACGACGAGACGAAGGTACACCTCATCTATGCCAATGTCACCTATGAGGACATTCTTTTAAAGGAGGAGTTGGATCTTCTTGCCAAAAGGTACCCAGATAGCTTCAAGCTCTACTATGTCTTAAACCAGCCTCCTGAAGAATGGGATGGTGGTGTCGGATTCGTATCGAAGGAGATGATTCAAACCCACTGCCCCGCTCCTGCTTCTGATATTCAGGTTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGGTGCTCATCTTGAAGCTCTTGGATATGCACCTGAGATGCTGTTCATGTTCTGAGCCTCCCTCAACCTCCACACTAGAGCTAAGCATCATTTATTTTTCTTCCCCCTTCTAAATTTTGAACATTATGCACTGAGAGGATGTTGCCTTAGCTCTTTTCACTTTGCAATGTTGTTGAACTTTGATGCCATCAATAACTTCAACTCTTAGAGCTAGTGAGAGTCTAGACTAGTTTTCATTCACATTGTTTGTTCATGTAACAATTCTAGAATGGTTTTCTTCTCGAAGTGTAATAAGACCGTTTAGATTAGCTTTTTAAGTGCTTGAAAACGCTTTTCTTTGCATCTAAAAGTCAAAGCCCAAGTCTACTAGTGGCAGAGATTGTCCGCTTTGGCTCATTTTGGCCCATTACGTACTGTCAGCTTCACGGGTTTTAAAATGTGTCTATTAGGGAGAGTTTTTCAAACTCTTATAAAGAATTATTTGTTTCCCTCTCCAGCCGATGTGAGATCTTATAATCCACCCTCCTTCAAGGCCCAGCATTCTCGTTGGCACACTGCTTAGTGTCTGGCTTTGATACCATTTGTAAGTCTACCGCTAGCAG

Coding sequence (CDS)

ATGGATCTCTCACAGTCTTTAGATCCGCAAGTCCTTCTGGGTTTGGCTGTGGCTGTTTTAGCCCTTGCCGTTGGTGCTCTCTATGTATTATCCTCGTCTAAAAAAAGCAAATCCAGAGAGGTATTCAAGGAGTTTAAACTTATCAAACGCGTGCAGCTGAGCCATAATGTGGCGAAGTTTACATTTACACTCCCAAATCCTACTTCAGTTTTGGGTCTTCCCATTGGACAACATATCAGTTGCAGGGGCAAGGATGGCCAAGGTGAAGAGGTCATCAAACCGTACACACCGATTACTTTAGATTCTGATGTTGGTTATTTTGAGCTCGTCATAAAGATGTATCCCCAAGGAAGGATGTCGCATCATTTCCGACAGATGCGTGTTGGCGATTTTCTTGCTGTAAAGGGGCCCAAGGGGCGTTTCAGGTATCAACCTGGGCAGGTCCGAGCATTTGGGATGCTCGCTGGAGGCTCCGGCATTACTCCTATGTATCAAGTTGCTCGAGCAATATTAGAAAATCCGAACGACGAGACGAAGGTACACCTCATCTATGCCAATGTCACCTATGAGGACATTCTTTTAAAGGAGGAGTTGGATCTTCTTGCCAAAAGGTACCCAGATAGCTTCAAGCTCTACTATGTCTTAAACCAGCCTCCTGAAGAATGGGATGGTGGTGTCGGATTCGTATCGAAGGAGATGATTCAAACCCACTGCCCCGCTCCTGCTTCTGATATTCAGGTTTTAAGATGTGGGCCACCACCAATGAACAAGGCCATGGGTGCTCATCTTGAAGCTCTTGGATATGCACCTGAGATGCTGTTCATGTTCTGA

Protein sequence

MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSREVFKEFKLIKRVQLSHNVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Homology
BLAST of Cp4.1LG10g10810 vs. ExPASy Swiss-Prot
Match: Q9ZNT1 (NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=1)

HSP 1 Score: 451.4 bits (1160), Expect = 7.1e-126
Identity = 220/275 (80.00%), Postives = 242/275 (88.00%), Query Frame = 0

Query: 5   QSLDPQVLLGLAVAVLALAVGALYVLSSSKKSK---SREVFKEFKLIKRVQLSHNVAKFT 64
           ++LD Q+LLG+ VA +A+  GA Y L+SSKK +     E FKEFKL+KR QLSHNVAKF 
Sbjct: 7   RTLDRQILLGVFVAFVAVGAGAAYFLTSSKKRRVCLDPENFKEFKLVKRHQLSHNVAKFV 66

Query: 65  FTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSH 124
           F LP  TSVLGLPIGQHISCRGKDGQGE+VIKPYTP TLDSDVG FELVIKMYPQGRMSH
Sbjct: 67  FELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQGRMSH 126

Query: 125 HFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDETKVH 184
           HFR+MRVGD LAVKGPKGRF+YQPGQ RAFGMLAGGSGITPM+QVARAILENP D+TKVH
Sbjct: 127 HFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTDKTKVH 186

Query: 185 LIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAP 244
           LIYANVTY+DILLKEEL+ L   YP+ FK++YVLNQPPE WDGGVGFVSKEMIQTHCPAP
Sbjct: 187 LIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQTHCPAP 246

Query: 245 ASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 277
           ASDIQ+LRCGPPPMNKAM A+LEALGY+PEM F F
Sbjct: 247 ASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

BLAST of Cp4.1LG10g10810 vs. ExPASy Swiss-Prot
Match: Q54NC1 (NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE=3 SV=1)

HSP 1 Score: 268.1 bits (684), Expect = 1.1e-70
Identity = 135/278 (48.56%), Postives = 187/278 (67.27%), Query Frame = 0

Query: 17  VAVLALAVGALYVLS----SSKKSKSREV--------------FKEFKLIKRVQLSHNVA 76
           +  +ALA G  YV +    S+K  K  E               +++F+L ++  ++HN  
Sbjct: 11  IGSVALAAGVKYVFTLTSGSNKDKKGGEAEKGKQVEKALDPQEYRKFQLKEKFIVNHNTR 70

Query: 77  KFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGR 136
            F F LPN   +LGLPIGQHIS R   G G+EV +PYTPI+ D + GYF+L+IK+Y +G 
Sbjct: 71  IFRFALPNEDDILGLPIGQHISLRAVVG-GKEVYRPYTPISSDEERGYFDLLIKVYEKGA 130

Query: 137 MSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDET 196
           MS +   M +GD + VKGPKG+F YQP   ++ GMLAGG+GITPM QV +AIL+NP+D+T
Sbjct: 131 MSGYVDNMFIGDSIEVKGPKGKFNYQPNMRKSIGMLAGGTGITPMLQVIKAILKNPSDKT 190

Query: 197 KVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHC 256
           ++ L++ N+T EDILLK+ELD LA+++P  FK+YYVLN PP+ W  GVGFVSKE+I++  
Sbjct: 191 EISLVFGNITEEDILLKKELDELAEKHP-QFKVYYVLNNPPKGWTQGVGFVSKEIIESRL 250

Query: 257 PAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 277
           P+P+    V+ CGPP MNKAM  HLE +G+    +F F
Sbjct: 251 PSPSDQTMVIMCGPPMMNKAMTGHLETIGFNESNIFTF 286

BLAST of Cp4.1LG10g10810 vs. ExPASy Swiss-Prot
Match: Q4X0B5 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / Af293 / CBS 101355 / FGSC A1100) OX=330879 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 266.9 bits (681), Expect = 2.5e-70
Identity = 133/261 (50.96%), Postives = 178/261 (68.20%), Query Frame = 0

Query: 16  AVAVLALAVGALYVLSSSKKSK--SREVFKEFKLIKRVQLSHNVAKFTFTLPNPTSVLGL 75
           AVAV A+  G       +K  K  +   F+EF L +++ +SHNV  + F LP PT +LGL
Sbjct: 34  AVAVTAILAGLKLFTGGNKPRKVLNPTEFQEFVLKEKIDVSHNVCIYRFALPRPTDILGL 93

Query: 76  PIGQHISCRGK-DGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFL 135
           PIGQHIS     +GQ +EV++ YTPI+ D++ GYF+L++K YPQG +S +   +++GD +
Sbjct: 94  PIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIGDTM 153

Query: 136 AVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILEN-----PNDETKVHLIYANV 195
            V+GPKG   Y P   R  GM+AGG+GITPM Q+ +A++ N      ND TK+ LI+ANV
Sbjct: 154 KVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIFANV 213

Query: 196 TYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASDIQV 255
             +DILLKEELD+LA   PD F +YYVLN PP+ W GGVGFV+ EMI+ H PAPASD+++
Sbjct: 214 NPDDILLKEELDMLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASDVKI 273

Query: 256 LRCGPPPMNKAMGAHLEALGY 269
           L CGPPPM  AM    E+LGY
Sbjct: 274 LLCGPPPMISAMKKATESLGY 293

BLAST of Cp4.1LG10g10810 vs. ExPASy Swiss-Prot
Match: A5DQ25 (NADH-cytochrome b5 reductase 1 OS=Meyerozyma guilliermondii (strain ATCC 6260 / CBS 566 / DSM 6381 / JCM 1539 / NBRC 10279 / NRRL Y-324) OX=294746 GN=CBR1 PE=3 SV=1)

HSP 1 Score: 264.6 bits (675), Expect = 1.2e-69
Identity = 134/269 (49.81%), Postives = 180/269 (66.91%), Query Frame = 0

Query: 3   LSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKS---KSREVFKEFKLIKRVQLSHNVAK 62
           +SQ+    +++   +A + LA  A+YV+  +KK+      +VF++F LI++ +LSHN   
Sbjct: 1   MSQNEPNPLVIFSTLAAIILAAVAVYVVKLNKKNGPVLKPDVFQKFPLIEKTRLSHNTCI 60

Query: 63  FTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRM 122
           + F LP  T  LGLPIGQHIS  G    G+EV++ YTPI+ D ++GYF+L+IK Y QG +
Sbjct: 61  YRFGLPKSTDRLGLPIGQHISI-GATINGKEVVRSYTPISRDDELGYFDLLIKTYEQGNI 120

Query: 123 SHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDETK 182
           S H     VGD + V+GPKG F Y P  V   GM+AGG+GI PMYQV  AIL NP+D+TK
Sbjct: 121 SRHVDSKSVGDHIEVRGPKGFFTYTPNMVEHLGMIAGGTGIAPMYQVLTAILTNPDDKTK 180

Query: 183 VHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCP 242
           + L+YANVT EDILL+ ELDL AK +PD FK++YVLN  PE W+G VGFV+ E+++ H P
Sbjct: 181 ISLVYANVTEEDILLRAELDLFAKEHPDRFKVHYVLNNAPENWNGSVGFVTPEIMEKHLP 240

Query: 243 APASDIQVLRCGPPPMNKAMGAHLEALGY 269
               D  +L CGPPPM  AM  +   LGY
Sbjct: 241 NKDQDGYLLLCGPPPMISAMKKNAVTLGY 268

BLAST of Cp4.1LG10g10810 vs. ExPASy Swiss-Prot
Match: A1DHW1 (NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3700 / CBS 544.65 / FGSC A1164 / JCM 1740 / NRRL 181 / WB 181) OX=331117 GN=cbr1 PE=3 SV=2)

HSP 1 Score: 262.7 bits (670), Expect = 4.7e-69
Identity = 132/261 (50.57%), Postives = 175/261 (67.05%), Query Frame = 0

Query: 16  AVAVLALAVGALYVLSSSKKSK--SREVFKEFKLIKRVQLSHNVAKFTFTLPNPTSVLGL 75
           AVA+ A+  G       SK  K  +   F+EF L ++  +SHNV  + F LP P  +LGL
Sbjct: 34  AVALTAILAGLKLFTGGSKPRKVLNPTEFQEFVLKEKTDISHNVCIYRFALPRPADILGL 93

Query: 76  PIGQHISCRGK-DGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFL 135
           PIGQHIS     +GQ +EV++ YTPI+ D++ GYF+L++K YPQG +S +   +++GD +
Sbjct: 94  PIGQHISLAATIEGQPKEVVRSYTPISSDNEAGYFDLLVKAYPQGNISKYLTTLKIGDTM 153

Query: 136 AVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILEN-----PNDETKVHLIYANV 195
            V+GPKG   Y P   R  GM+AGG+GITPM Q+ +A++ N      ND TK+ LI+ANV
Sbjct: 154 KVRGPKGAMVYTPNMCRHIGMIAGGTGITPMLQIIKAVIRNRPRNGGNDTTKLDLIFANV 213

Query: 196 TYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASDIQV 255
             +DILLKEELD LA   PD F +YYVLN PP+ W GGVGFV+ EMI+ H PAPASD+++
Sbjct: 214 NPDDILLKEELDKLAAEDPD-FNIYYVLNNPPQGWTGGVGFVTPEMIKEHLPAPASDVKI 273

Query: 256 LRCGPPPMNKAMGAHLEALGY 269
           L CGPPPM  AM    E+LGY
Sbjct: 274 LLCGPPPMISAMKKATESLGY 293

BLAST of Cp4.1LG10g10810 vs. NCBI nr
Match: XP_023543481.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 548 bits (1412), Expect = 1.54e-196
Identity = 276/281 (98.22%), Postives = 276/281 (98.22%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR     EVFKEFKLIKRVQLSH
Sbjct: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEVFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. NCBI nr
Match: KAG7033625.1 (NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 543 bits (1398), Expect = 2.10e-194
Identity = 272/281 (96.80%), Postives = 274/281 (97.51%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD  QSLDPQVLLGLAVAVLALAVG+LYVLSSSKKSKSR     E+FKEFKLIKRVQLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. NCBI nr
Match: KAG6603441.1 (NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 541 bits (1394), Expect = 8.55e-194
Identity = 271/281 (96.44%), Postives = 273/281 (97.15%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD  QSLDPQVLLGLAVAVLALAVG+LYVLSSSKKSKSR     E+FKEFKLIKRVQLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMR GDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRAGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. NCBI nr
Match: XP_022950742.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita moschata])

HSP 1 Score: 540 bits (1392), Expect = 1.73e-193
Identity = 271/281 (96.44%), Postives = 273/281 (97.15%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD  QSLDPQVLLGLAVAVLALAVG+LYVLSSSKKSKSR     E+FKEFKLIKRVQLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. NCBI nr
Match: XP_022967875.1 (NADH--cytochrome b5 reductase 1-like [Cucurbita maxima])

HSP 1 Score: 537 bits (1384), Expect = 2.86e-192
Identity = 269/281 (95.73%), Postives = 272/281 (96.80%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD SQSLDPQ+LLGLAVA LAL VGALYVLSSSKKSKSR     E+FKEFKLIKRVQLSH
Sbjct: 1   MDFSQSLDPQILLGLAVAGLALVVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVG+FLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGNFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQVLRCGPPPMNKAM AHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. ExPASy TrEMBL
Match: A0A6J1GFP5 (NADH--cytochrome b5 reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111453751 PE=4 SV=1)

HSP 1 Score: 540 bits (1392), Expect = 8.35e-194
Identity = 271/281 (96.44%), Postives = 273/281 (97.15%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD  QSLDPQVLLGLAVAVLALAVG+LYVLSSSKKSKSR     E+FKEFKLIKRVQLSH
Sbjct: 1   MDFLQSLDPQVLLGLAVAVLALAVGSLYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAK YPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKSYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. ExPASy TrEMBL
Match: A0A6J1HVM7 (NADH--cytochrome b5 reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467252 PE=4 SV=1)

HSP 1 Score: 537 bits (1384), Expect = 1.38e-192
Identity = 269/281 (95.73%), Postives = 272/281 (96.80%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD SQSLDPQ+LLGLAVA LAL VGALYVLSSSKKSKSR     E+FKEFKLIKRVQLSH
Sbjct: 1   MDFSQSLDPQILLGLAVAGLALVVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRVQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVG+FLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGNFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ
Sbjct: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQVLRCGPPPMNKAM AHLEALGYAPEMLFMF
Sbjct: 241 THCPAPASDIQVLRCGPPPMNKAMAAHLEALGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. ExPASy TrEMBL
Match: A0A5A7TLD5 (NADH--cytochrome b5 reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G001310 PE=4 SV=1)

HSP 1 Score: 533 bits (1374), Expect = 4.63e-191
Identity = 266/281 (94.66%), Postives = 271/281 (96.44%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD  QSLDPQ+LLGLAVAVLALAVGALYVLSSSKKSKSR     E+FKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQ+LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. ExPASy TrEMBL
Match: A0A1S3C2D1 (NADH--cytochrome b5 reductase 1 OS=Cucumis melo OX=3656 GN=LOC103495874 PE=4 SV=1)

HSP 1 Score: 533 bits (1374), Expect = 4.63e-191
Identity = 266/281 (94.66%), Postives = 271/281 (96.44%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD  QSLDPQ+LLGLAVAVLALAVGALYVLSSSKKSKSR     E+FKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQILLGLAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEVWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQ+LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. ExPASy TrEMBL
Match: A0A0A0L226 (FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G416480 PE=4 SV=1)

HSP 1 Score: 528 bits (1361), Expect = 4.44e-189
Identity = 263/281 (93.59%), Postives = 270/281 (96.09%), Query Frame = 0

Query: 1   MDLSQSLDPQVLLGLAVAVLALAVGALYVLSSSKKSKSR-----EVFKEFKLIKRVQLSH 60
           MD  QSLDPQ+LLG+AVAVLALAVGALYVLSSSKKSKSR     E+FKEFKLIKR+QLSH
Sbjct: 1   MDFLQSLDPQILLGVAVAVLALAVGALYVLSSSKKSKSRGCLDPEIFKEFKLIKRLQLSH 60

Query: 61  NVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120
           NVAKFTFTLP+PTSVLGLPIGQHISC GKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP
Sbjct: 61  NVAKFTFTLPSPTSVLGLPIGQHISCSGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYP 120

Query: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180
           QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN
Sbjct: 121 QGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPN 180

Query: 181 DETKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQ 240
           D+TKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPE WDGGVGFVSKEMIQ
Sbjct: 181 DKTKVHLIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEAWDGGVGFVSKEMIQ 240

Query: 241 THCPAPASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 276
           THCPAPASDIQ+LRCGPPPMNKAM AHLE LGYAPEMLFMF
Sbjct: 241 THCPAPASDIQILRCGPPPMNKAMAAHLEELGYAPEMLFMF 281

BLAST of Cp4.1LG10g10810 vs. TAIR 10
Match: AT5G17770.1 (NADH:cytochrome B5 reductase 1 )

HSP 1 Score: 451.4 bits (1160), Expect = 5.1e-127
Identity = 220/275 (80.00%), Postives = 242/275 (88.00%), Query Frame = 0

Query: 5   QSLDPQVLLGLAVAVLALAVGALYVLSSSKKSK---SREVFKEFKLIKRVQLSHNVAKFT 64
           ++LD Q+LLG+ VA +A+  GA Y L+SSKK +     E FKEFKL+KR QLSHNVAKF 
Sbjct: 7   RTLDRQILLGVFVAFVAVGAGAAYFLTSSKKRRVCLDPENFKEFKLVKRHQLSHNVAKFV 66

Query: 65  FTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVGYFELVIKMYPQGRMSH 124
           F LP  TSVLGLPIGQHISCRGKDGQGE+VIKPYTP TLDSDVG FELVIKMYPQGRMSH
Sbjct: 67  FELPTSTSVLGLPIGQHISCRGKDGQGEDVIKPYTPTTLDSDVGRFELVIKMYPQGRMSH 126

Query: 125 HFRQMRVGDFLAVKGPKGRFRYQPGQVRAFGMLAGGSGITPMYQVARAILENPNDETKVH 184
           HFR+MRVGD LAVKGPKGRF+YQPGQ RAFGMLAGGSGITPM+QVARAILENP D+TKVH
Sbjct: 127 HFREMRVGDHLAVKGPKGRFKYQPGQFRAFGMLAGGSGITPMFQVARAILENPTDKTKVH 186

Query: 185 LIYANVTYEDILLKEELDLLAKRYPDSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAP 244
           LIYANVTY+DILLKEEL+ L   YP+ FK++YVLNQPPE WDGGVGFVSKEMIQTHCPAP
Sbjct: 187 LIYANVTYDDILLKEELEGLTTNYPEQFKIFYVLNQPPEVWDGGVGFVSKEMIQTHCPAP 246

Query: 245 ASDIQVLRCGPPPMNKAMGAHLEALGYAPEMLFMF 277
           ASDIQ+LRCGPPPMNKAM A+LEALGY+PEM F F
Sbjct: 247 ASDIQILRCGPPPMNKAMAANLEALGYSPEMQFQF 281

BLAST of Cp4.1LG10g10810 vs. TAIR 10
Match: AT5G20080.1 (FAD/NAD(P)-binding oxidoreductase )

HSP 1 Score: 207.6 bits (527), Expect = 1.3e-53
Identity = 107/261 (41.00%), Postives = 159/261 (60.92%), Query Frame = 0

Query: 34  KKSKSREVFKEFKLIKRVQLSHNVAKFTFTLPNPTSVLGLPIGQHISCR-----GKDGQG 93
           K + + + + EFKL    ++SHN   F F+  +P++ LGL +   +  R       +G+ 
Sbjct: 70  KTALNPDKWLEFKLQDTARVSHNTQLFRFSF-DPSAELGLHVASCLLTRAPLGYNAEGKT 129

Query: 94  EEVIKPYTPITLDSDVGYFELVIKMYPQGRMSHHFRQMRVGDFLAVKGPKGRFRYQPGQV 153
           + VI+PYTPI+     GYF+L+IK+YP G+MS HF  ++ GD L VKGP  +F+Y P   
Sbjct: 130 KYVIRPYTPISDPEAKGYFDLLIKVYPDGKMSQHFASLKPGDVLEVKGPVEKFKYSPNMK 189

Query: 154 RAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANVTYEDILLKEELDLLAKRYPDS 213
           +  GM+AGGSGITPM QV  AI++NP D T++ L+YANV+ +DILLK++LD+L   +P +
Sbjct: 190 KHIGMIAGGSGITPMLQVIDAIVKNPEDNTQISLLYANVSPDDILLKQKLDVLQANHP-N 249

Query: 214 FKLYYVLNQPPEEWDGGVGFVSKEMIQTHCPAPASDIQVLRCGPPPMNKAMGAH------ 273
            K++Y ++ P + W GGVG++SK+M     P P  D  +L CGPP M + +         
Sbjct: 250 LKIFYTVDNPTKNWKGGVGYISKDMALKGLPLPTDDTLILVCGPPGMMEHISGGKAPDWS 309

Query: 274 -------LEALGYAPEMLFMF 277
                  L+ LGY  EM+F F
Sbjct: 310 QGEVKGILKELGYTEEMVFKF 328

BLAST of Cp4.1LG10g10810 vs. TAIR 10
Match: AT1G37130.1 (nitrate reductase 2 )

HSP 1 Score: 173.7 bits (439), Expect = 2.0e-43
Identity = 97/244 (39.75%), Postives = 143/244 (58.61%), Query Frame = 0

Query: 46  KLIKRVQLSHNVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVG 105
           +L+++  +SH+V KF F LP    VLGLP+G+HI         +  ++ YTP +    VG
Sbjct: 666 QLVEKTSISHDVRKFRFALPVEDMVLGLPVGKHIFLCATIND-KLCLRAYTPSSTVDVVG 725

Query: 106 YFELVIKMY-----PQ----GRMSHHFRQMRVGDFLAVKGPKGRFRY----------QPG 165
           YFELV+K+Y     P+    G MS +   + +G  L +KGP G   Y          +P 
Sbjct: 726 YFELVVKIYFGGVHPRFPNGGLMSQYLDSLPIGSTLEIKGPLGHVEYLGKGSFTVHGKPK 785

Query: 166 QVRAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANVTYEDILLKEELDLLAKRYP 225
                 MLAGG+GITP+YQ+ +AIL++P DET++++IYAN T EDILL+EELD  A++YP
Sbjct: 786 FADKLAMLAGGTGITPVYQIIQAILKDPEDETEMYVIYANRTEEDILLREELDGWAEQYP 845

Query: 226 DSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCP-APASDIQVLRCGPPPMNK-AMGAHLE 269
           D  K++YV+    E W    GF+S+ +++ H P         + CGPPPM + A+  +LE
Sbjct: 846 DRLKVWYVVESAKEGWAYSTGFISEAIMREHIPDGLDGSALAMACGPPPMIQFAVQPNLE 905

BLAST of Cp4.1LG10g10810 vs. TAIR 10
Match: AT1G77760.1 (nitrate reductase 1 )

HSP 1 Score: 165.2 bits (417), Expect = 7.3e-41
Identity = 86/244 (35.25%), Postives = 142/244 (58.20%), Query Frame = 0

Query: 46  KLIKRVQLSHNVAKFTFTLPNPTSVLGLPIGQHISCRGKDGQGEEVIKPYTPITLDSDVG 105
           +LI++  +SH+V KF F LP+    LGLP+G+H+         +  ++ YTP +    VG
Sbjct: 666 RLIEKTSISHDVRKFRFALPSEDQQLGLPVGKHVFVCANIND-KLCLRAYTPTSAIDAVG 725

Query: 106 YFELVIKMY-----PQ----GRMSHHFRQMRVGDFLAVKGPKGRFRY----------QPG 165
           + +LV+K+Y     P+    G MS H   + +G  + +KGP G   Y          +P 
Sbjct: 726 HIDLVVKVYFKDVHPRFPNGGLMSQHLDSLPIGSMIDIKGPLGHIEYKGKGNFLVSGKPK 785

Query: 166 QVRAFGMLAGGSGITPMYQVARAILENPNDETKVHLIYANVTYEDILLKEELDLLAKRYP 225
             +   MLAGG+GITP+YQ+ ++IL +P DET+++++YAN T +DIL++EEL+  A ++ 
Sbjct: 786 FAKKLAMLAGGTGITPIYQIIQSILSDPEDETEMYVVYANRTEDDILVREELEGWASKHK 845

Query: 226 DSFKLYYVLNQPPEEWDGGVGFVSKEMIQTHCP-APASDIQVLRCGPPPMNK-AMGAHLE 269
           +  K++YV+    E W    GF+++ +++ H P     +   L CGPPPM + A+  +LE
Sbjct: 846 ERLKIWYVVEIAKEGWSYSTGFITEAVLREHIPEGLEGESLALACGPPPMIQFALQPNLE 905

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZNT17.1e-12680.00NADH--cytochrome b5 reductase 1 OS=Arabidopsis thaliana OX=3702 GN=CBR1 PE=1 SV=... [more]
Q54NC11.1e-7048.56NADH-cytochrome b5 reductase 1 OS=Dictyostelium discoideum OX=44689 GN=cyb5r1 PE... [more]
Q4X0B52.5e-7050.96NADH-cytochrome b5 reductase 1 OS=Neosartorya fumigata (strain ATCC MYA-4609 / A... [more]
A5DQ251.2e-6949.81NADH-cytochrome b5 reductase 1 OS=Meyerozyma guilliermondii (strain ATCC 6260 / ... [more]
A1DHW14.7e-6950.57NADH-cytochrome b5 reductase 1 OS=Neosartorya fischeri (strain ATCC 1020 / DSM 3... [more]
Match NameE-valueIdentityDescription
XP_023543481.11.54e-19698.22NADH--cytochrome b5 reductase 1-like [Cucurbita pepo subsp. pepo][more]
KAG7033625.12.10e-19496.80NADH--cytochrome b5 reductase 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6603441.18.55e-19496.44NADH--cytochrome b5 reductase 1, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022950742.11.73e-19396.44NADH--cytochrome b5 reductase 1-like [Cucurbita moschata][more]
XP_022967875.12.86e-19295.73NADH--cytochrome b5 reductase 1-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1GFP58.35e-19496.44NADH--cytochrome b5 reductase 1-like OS=Cucurbita moschata OX=3662 GN=LOC1114537... [more]
A0A6J1HVM71.38e-19295.73NADH--cytochrome b5 reductase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111467252... [more]
A0A5A7TLD54.63e-19194.66NADH--cytochrome b5 reductase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3C2D14.63e-19194.66NADH--cytochrome b5 reductase 1 OS=Cucumis melo OX=3656 GN=LOC103495874 PE=4 SV=... [more]
A0A0A0L2264.44e-18993.59FAD-binding FR-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_... [more]
Match NameE-valueIdentityDescription
AT5G17770.15.1e-12780.00NADH:cytochrome B5 reductase 1 [more]
AT5G20080.11.3e-5341.00FAD/NAD(P)-binding oxidoreductase [more]
AT1G37130.12.0e-4339.75nitrate reductase 2 [more]
AT1G77760.17.3e-4135.25nitrate reductase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001834NADH:cytochrome b5 reductase-likePRINTSPR00406CYTB5RDTASEcoord: 93..100
score: 41.41
coord: 246..254
score: 62.96
coord: 71..82
score: 57.47
coord: 152..171
score: 66.67
coord: 190..201
score: 64.06
coord: 129..143
score: 49.31
IPR001433Oxidoreductase FAD/NAD(P)-bindingPFAMPF00175NAD_binding_1coord: 153..258
e-value: 7.6E-31
score: 107.0
IPR008333Flavoprotein pyridine nucleotide cytochrome reductase-like, FAD-binding domainPFAMPF00970FAD_binding_6coord: 45..143
e-value: 7.6E-32
score: 109.6
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainGENE3D3.40.50.80coord: 135..276
e-value: 6.9E-45
score: 154.4
IPR039261Ferredoxin-NADP reductase (FNR), nucleotide-binding domainSUPERFAMILY52343Ferredoxin reductase-like, C-terminal NADP-linked domaincoord: 132..275
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 37..134
e-value: 4.5E-33
score: 115.9
NoneNo IPR availablePANTHERPTHR19370:SF203NADH-CYTOCHROME B5 REDUCTASEcoord: 5..276
NoneNo IPR availablePANTHERPTHR19370NADH-CYTOCHROME B5 REDUCTASEcoord: 5..276
NoneNo IPR availableCDDcd06183cyt_b5_reduct_likecoord: 45..276
e-value: 1.2231E-118
score: 337.617
IPR017927FAD-binding domain, ferredoxin reductase-typePROSITEPS51384FAD_FRcoord: 40..144
score: 14.326914
IPR017938Riboflavin synthase-like beta-barrelSUPERFAMILY63380Riboflavin synthase domain-likecoord: 40..145

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g10810.1Cp4.1LG10g10810.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0022900 electron transport chain
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0004128 cytochrome-b5 reductase activity, acting on NAD(P)H
molecular_function GO:0009703 nitrate reductase (NADH) activity
molecular_function GO:0016491 oxidoreductase activity