Cp4.1LG10g10680 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG10g10680
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionE3 ubiquitin-protein ligase
LocationCp4.1LG10: 6689812 .. 6693079 (-)
RNA-Seq ExpressionCp4.1LG10g10680
SyntenyCp4.1LG10g10680
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CTCTCACTGGAACTCCTCGCTTTTTTCACCGGCGCCTTTTCTCCTCTCTCCGATCATCTCCACGTTCTCTCTCTCCGTCGGGTTTGGCTTTTTATGGACGGCGGCAGACCATCGAGCACCGGGTTCTTGTCCTGGCGGCGGACCTTGAGCTCGAGATTCGCGCTTCCTTTGATTCAAGACGCCGCCAATTTGATCATGGAACATCGCGGCGTAATCTTCGGTGACTGTGCCGAACCACGAGTTGATGATGAGGAGGATGAGAATGGATACGACCGTCGGGTTTTTTTGCGGAATGGGTCGTCCTTCATCAAGCCTATTTTGGTACTCGACTTGTTCTGGAATTTGTTCTTCGTTTTTGTGTCCGTGATTGTGCTCGTACTGTCTGCTGAAGAGAAACCTTCCGCTCCTTTGAGATTATGGCTTTCGGGTTATGCTGTCCAGTGCCTTCTCCATGTCTTTTTTGTGTTCTTTGCCTACTTTAGAAGGAGTTCTCGCTATCGATTTGTGTTTGAGAATCGTGTGGCTCAGGATGAACTTCGCCTTTCTCATAATCGAATAAGGTAACTTTTTCTTTTCGGAAAAGTTTGTATGGGGGATTTTTTGTTGGAATTGATTTCTCTTTTTTTTTTTTTTTTTTTTTTTNCATTTATTGGGTTCTTAGAATGATTGTGGTGCCAGTAGTGAAGATTATCTGCTAGTTTATGGCAATTTTTATCAAATCGCTTACGATCAGTATAGTTAACTATTGGATTCTACGCATGTATCATCCCATTTATTTCATGTTCTTCATGGATTTAGGAAATAAAAAACCACTCAAGTTGTTGGAATGGCGAGTTCATCTTTCCATATTGGCTGTAGATCTTTCTTAATTGGTCGATTGATTATCATGTGGTTTTCTTTTAGATGTAACGCTAACTTGATTAATCGAGTTCTTCTAGTTTAATTTCGAAGGGATTCTGTATTTTGAACTTGATTAAGAATTTTTATTTTATTATGGCAACAACAATTCTTCTAGCTTTTTTCTTAAAGGTTCATTGGATTAGGTTCTTATTTCCATGTCTTGGAAGGATTTCACACTTAACTAGCAGGAACTTGTTTCCCATCAAGATATTTGCATGTTTACTTATTGTTCTTCTTCTGCCCTTACATATGGAGGTGTTATCTATCTTTCTATTTACCTCTCTGTTTTTTTCCCTTAGATTTTTGAAAAGATTGGAAGCATCGAACACAATGATATCATATATCTGGTGGGTTTTTGGATTCTATTGGATTGTCACGGGTGGTCAATCTCTTTTGGAAGGCTCTCCCCGGCTCTACTGGTAAATGCTCCCTCCTAAGATCTTGAAATCCTTTTTTCTCTTGCTAAAGTCGTCTATGGTTTAAGATCAACTTAGTAGAATTATGATATAAACACATCTTGAACTATACGACCATGCTGATGGAAAGGATTGACCCGCTGAAGTATCTCTTTGAAAAGCAATGCCTGTGTATTTATTTATCTCGATTCTTATATTACTTGCGAATGTGCGAATGAAAGCTGTCAAGACTGAAAAAAGTATTCAGGCTATGTTATCATATTATAGTTTAGCTAGTTTTGGTTTCTTCTTGTGTGCCTTTGGCTGTCTCCTGTGGGTTCGGCATTGTTTCGTGCTGTTGGCTATTTGTTTATGTTGTTTTAATCGTTTTATAGTCGTTTTTGTGTGTGGAAACTTCTCATTTAACTTGATAGAAGTCACATATGAGGTCTCGCTAATGGACTCATGATATGAACCGCAGGTTGGCAGTGGTTTTCCTAGCCTTTGATGTGTTCTTTATTATCTTCTGCACTGGGATGGCTTTTGTTGGTTTCTTTACTGTTTGCTGCATAATACCATTTTTAGCATATGGCTATACCATGACATTCCTAGAAGGTGCCTCTGAAGATGAAATTAGAGCACTTCCCAAGTACAGGTTTCGCCAAGACAATCCACTCGAGTCGTTCGACAGTGACAAGAAACATGAAGTTGGGATGACCTTGGAGCCTGGCTACAATGGTTATACAACTGAACACCTTCTAAATACAGAGGACTCGGTGAGAAACCGTCTTATTTTTTACTTACATTCTATTTTCATCTATTAGGAACCAACATAGTAAATGAAAACACTCTTGCAACGGTCTAAGCCTATCGCTAGCAGATGTCCTCTTTGGGCTTACCCTCAAGGTTTCTAAAACGCGTCAGTTAGGAAGAGGTTTCCACACCCTTATAAGGAATGTTTCGTTCTCCTCCCCAACTGATGTGGGATCTCACATCTTCAAACTCATAAAATAGACGTCTTGTATCATTCCTTGAACAAAATTTTCCTATTTAAACATATGCCTATCGACCTGTTAACTATTGTTATACATGTAGGTCTTGTAGCGAAACAAGGTTATCCGTGATACTGTTGCAAAATAGTTTGAGTTTTTCTTTAAATTCTTTATCCGACTCAAATAGAGTCACGGTTCCTTGTTTCGGAATGACGTTGTTTTTTCCTCGTAATGCCTTTGTTATGTAGCCTTGTAGGACTCTTTGCTACGTGATTAACTCTACGTTGTATTATAATCGTAGGCATGCTGCATATGCCTTGCCCAGTATGTACATGGAGTACAATTGTGCATGCTTCCCTGTAGTCATCATTTCCATACTCGATGCATTGTCAAATGGCTTCGAATAAACGCAACCTGCCCCCTCTGCAAGTTCAGCATCGGACATGGCGATTCCTTGGTCTGATTCACATATCGATCGAGTTGGAGCATTGTCAAGATCTCCCGAGATCTCAAATGGAAACACACACAAACACAGAATGACATGAATCATCTCTTGGAAAAAAGGTACAAATTTCCTAAAGGGTCACTCAAAGTGTAGATAGGTTCTTCTTTTTCTTGTTCCCTTTCCCTTTTAGATTACTTAGGAGATCATATCAATGGGGAATACTGTGAATATACACTTCGATGCACAGCTACTAACTTATATGTCACTAAGAGACCACTTAGTTGATAGTAACATTTAGTGGATAAAGGCAAAAAGAAGCCATTTGTTTACTCATAACCTCATCTCATCTCATCTCATCTCATCTGAAAAGAGCTTTTAAAGGCTGAGTGTGTTGGGTATGCGTTTAATGGATGGTTTTAGTGGAGATTGTGGAATTGGCAGCCATATTGTATTGGTTTTTGTTAGTAATCTATGTCTTTAATTTGTTTTTTTTATCTATTGGTTTAAGGGAAGTGTTTGTGGGGATTCTTTTTTTA

mRNA sequence

CTCTCACTGGAACTCCTCGCTTTTTTCACCGGCGCCTTTTCTCCTCTCTCCGATCATCTCCACGTTCTCTCTCTCCGTCGGGTTTGGCTTTTTATGGACGGCGGCAGACCATCGAGCACCGGGTTCTTGTCCTGGCGGCGGACCTTGAGCTCGAGATTCGCGCTTCCTTTGATTCAAGACGCCGCCAATTTGATCATGGAACATCGCGGCGTAATCTTCGGTGACTGTGCCGAACCACGAGTTGATGATGAGGAGGATGAGAATGGATACGACCGTCGGGTTTTTTTGCGGAATGGGTCGTCCTTCATCAAGCCTATTTTGGTACTCGACTTGTTCTGGAATTTGTTCTTCGTTTTTGTGTCCGTGATTGTGCTCGTACTGTCTGCTGAAGAGAAACCTTCCGCTCCTTTGAGATTATGGCTTTCGGGTTATGCTGTCCAGTGCCTTCTCCATGTCTTTTTTGTGTTCTTTGCCTACTTTAGAAGGAGTTCTCGCTATCGATTTGTGTTTGAGAATCGTGTGGCTCAGGATGAACTTCGCCTTTCTCATAATCGAATAAGATTTTTGAAAAGATTGGAAGCATCGAACACAATGATATCATATATCTGGTGGGTTTTTGGATTCTATTGGATTGTCACGGGTGGTCAATCTCTTTTGGAAGGCTCTCCCCGGCTCTACTGGTTGGCAGTGGTTTTCCTAGCCTTTGATGTGTTCTTTATTATCTTCTGCACTGGGATGGCTTTTGTTGGTTTCTTTACTGTTTGCTGCATAATACCATTTTTAGCATATGGCTATACCATGACATTCCTAGAAGGTGCCTCTGAAGATGAAATTAGAGCACTTCCCAAGTACAGGTTTCGCCAAGACAATCCACTCGAGTCGTTCGACAGTGACAAGAAACATGAAGTTGGGATGACCTTGGAGCCTGGCTACAATGGTTATACAACTGAACACCTTCTAAATACAGAGGACTCGGCATGCTGCATATGCCTTGCCCAGTATGTACATGGAGTACAATTGTGCATGCTTCCCTGTAGTCATCATTTCCATACTCGATGCATTGTCAAATGGCTTCGAATAAACGCAACCTGCCCCCTCTGCAAGTTCAGCATCGGACATGGCGATTCCTTGGTCTGATTCACATATCGATCGAGTTGGAGCATTGTCAAGATCTCCCGAGATCTCAAATGGAAACACACACAAACACAGAATGACATGAATCATCTCTTGGAAAAAAGGTACAAATTTCCTAAAGGGTCACTCAAAGTGTAGATAGGTTCTTCTTTTTCTTGTTCCCTTTCCCTTTTAGATTACTTAGGAGATCATATCAATGGGGAATACTGTGAATATACACTTCGATGCACAGCTACTAACTTATATGTCACTAAGAGACCACTTAGTTGATAGTAACATTTAGTGGATAAAGGCAAAAAGAAGCCATTTGTTTACTCATAACCTCATCTCATCTCATCTCATCTCATCTGAAAAGAGCTTTTAAAGGCTGAGTGTGTTGGGTATGCGTTTAATGGATGGTTTTAGTGGAGATTGTGGAATTGGCAGCCATATTGTATTGGTTTTTGTTAGTAATCTATGTCTTTAATTTGTTTTTTTTATCTATTGGTTTAAGGGAAGTGTTTGTGGGGATTCTTTTTTTA

Coding sequence (CDS)

ATGGACGGCGGCAGACCATCGAGCACCGGGTTCTTGTCCTGGCGGCGGACCTTGAGCTCGAGATTCGCGCTTCCTTTGATTCAAGACGCCGCCAATTTGATCATGGAACATCGCGGCGTAATCTTCGGTGACTGTGCCGAACCACGAGTTGATGATGAGGAGGATGAGAATGGATACGACCGTCGGGTTTTTTTGCGGAATGGGTCGTCCTTCATCAAGCCTATTTTGGTACTCGACTTGTTCTGGAATTTGTTCTTCGTTTTTGTGTCCGTGATTGTGCTCGTACTGTCTGCTGAAGAGAAACCTTCCGCTCCTTTGAGATTATGGCTTTCGGGTTATGCTGTCCAGTGCCTTCTCCATGTCTTTTTTGTGTTCTTTGCCTACTTTAGAAGGAGTTCTCGCTATCGATTTGTGTTTGAGAATCGTGTGGCTCAGGATGAACTTCGCCTTTCTCATAATCGAATAAGATTTTTGAAAAGATTGGAAGCATCGAACACAATGATATCATATATCTGGTGGGTTTTTGGATTCTATTGGATTGTCACGGGTGGTCAATCTCTTTTGGAAGGCTCTCCCCGGCTCTACTGGTTGGCAGTGGTTTTCCTAGCCTTTGATGTGTTCTTTATTATCTTCTGCACTGGGATGGCTTTTGTTGGTTTCTTTACTGTTTGCTGCATAATACCATTTTTAGCATATGGCTATACCATGACATTCCTAGAAGGTGCCTCTGAAGATGAAATTAGAGCACTTCCCAAGTACAGGTTTCGCCAAGACAATCCACTCGAGTCGTTCGACAGTGACAAGAAACATGAAGTTGGGATGACCTTGGAGCCTGGCTACAATGGTTATACAACTGAACACCTTCTAAATACAGAGGACTCGGCATGCTGCATATGCCTTGCCCAGTATGTACATGGAGTACAATTGTGCATGCTTCCCTGTAGTCATCATTTCCATACTCGATGCATTGTCAAATGGCTTCGAATAAACGCAACCTGCCCCCTCTGCAAGTTCAGCATCGGACATGGCGATTCCTTGGTCTGA

Protein sequence

MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYDRRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLEGASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV
Homology
BLAST of Cp4.1LG10g10680 vs. ExPASy Swiss-Prot
Match: Q8LDB8 (E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g63170 PE=2 SV=2)

HSP 1 Score: 239.6 bits (610), Expect = 5.4e-62
Identity = 132/302 (43.71%), Postives = 181/302 (59.93%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYF 129
           ++ KP++VLD  WNL FV V+  VLVLS++E P+ PLR+W+ GY +QC++H+  V   Y 
Sbjct: 69  AYSKPVVVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYR 128

Query: 130 RRSSRYRFVFENRVA------------QDELRLSHN--------------RIRFLKRLEA 189
           RR+SR R     R +            ++ L LS N                 F K LE+
Sbjct: 129 RRNSRRRRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLES 188

Query: 190 SNTMISYIWWVFGFYWIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTV 249
           +NTMIS+IWWV GFYW+ +GGQ L +GSP+LYWL +VFL FDVFF++FC  +A V    V
Sbjct: 189 ANTMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAV 248

Query: 250 CCIIP-FLAYGYTMTFLEGASEDEIRALPKYRFRQ--DNPLESFDSDKKH--EVGMTLEP 309
           CC +P  +A  Y +   EGAS+++I  L K++FR+  D    + D ++      G+  E 
Sbjct: 249 CCCLPCIIAVLYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTEC 308

Query: 310 GYNGYTTEHLLNTEDSACCICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKF 341
           G +    EH L  ED+ CCICL+ Y    +L  LPC HHFH  C+ KWL INATCPLCK+
Sbjct: 309 GTDS-PVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKY 368

BLAST of Cp4.1LG10g10680 vs. ExPASy Swiss-Prot
Match: Q93Z92 (E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g11680 PE=2 SV=1)

HSP 1 Score: 235.7 bits (600), Expect = 7.7e-61
Identity = 124/283 (43.82%), Postives = 172/283 (60.78%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYF 129
           ++ KP++ LD+ WNL FV + V VL+LS +EKP+ PLR+W+ GY +QC LH+  V   Y 
Sbjct: 104 AYSKPVVFLDILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYR 163

Query: 130 RRSSRYR------FVFENRVAQDELRLSHNRIRF-----LKRLEASNTMISYIWWVFGFY 189
           RR  R            N  +Q  + L+    R       K LE++NTM S+IWW+ GFY
Sbjct: 164 RRRRRRHPEDGGGSGLTNSSSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGFY 223

Query: 190 WIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIP-FLAYGYTMT 249
           W+  GGQ+L   SP+LYWL ++FL FDVFF++FC  +A V    VCC +P  +A  Y + 
Sbjct: 224 WVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAVA 283

Query: 250 FLEGASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACC 309
             EGAS+++I  +PK+RF +   +E      +   G+  E G +    E  L+ ED+ CC
Sbjct: 284 DQEGASKNDIDQMPKFRFTKTGNVEKLSGKAR---GIMTECGTDS-PIERSLSPEDAECC 343

Query: 310 ICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSI 341
           ICL +Y  GV+L  LPC+HHFH  CI KWL IN+ CPLCKF+I
Sbjct: 344 ICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of Cp4.1LG10g10680 vs. ExPASy Swiss-Prot
Match: Q9LN71 (E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g12760 PE=1 SV=1)

HSP 1 Score: 229.6 bits (584), Expect = 5.5e-59
Identity = 127/304 (41.78%), Postives = 174/304 (57.24%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYF 129
           ++ KP++VLD+ WNL FV V+  +LV+S +E P  PLR+WL GYA+QC+LH+  V   Y 
Sbjct: 95  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 154

Query: 130 RR---------------------SSRYRFVFENRVAQ------DELRLSH---NRIRFLK 189
           RR                     SS    + E  +         +L L H         K
Sbjct: 155 RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 214

Query: 190 RLEASNTMISYIWWVFGFYWIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVG 249
            LE++NTM S+IWW+ GFYW+  GGQ L + SPR+YWL++VFL FDVFF++FC  +A V 
Sbjct: 215 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 274

Query: 250 FFTVCCIIP-FLAYGYTMTFLEGASEDEIRALPKYRFRQDNPLESFDSDKKHEV--GMTL 309
              VCC +P  +A  Y +   EGAS+++I  L K++FR+        +D+      G+  
Sbjct: 275 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMT 334

Query: 310 EPGYNGYTTEHLLNTEDSACCICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLC 341
           E G +    EH L  ED+ CCICL+ Y  G +L  LPC HHFH  C+ KWL INATCPLC
Sbjct: 335 ECGTDS-PIEHTLLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 394

BLAST of Cp4.1LG10g10680 vs. ExPASy Swiss-Prot
Match: Q8GUU2 (E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 2.8e-58
Identity = 122/295 (41.36%), Postives = 174/295 (58.98%), Query Frame = 0

Query: 71  FIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYFR 130
           + KP++  D+ WN   V  S ++LV + EE+P+ P+R+W+  Y +QCL HV  V+  Y+R
Sbjct: 73  YSKPVVAADILWNAALVLASAVMLVGTVEERPNEPIRVWICVYGLQCLFHVVLVWSEYWR 132

Query: 131 RSSR-------------YRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGF 190
           R+S              Y   ++     D+   +++   F+KR E+ NT+IS+IWW+ GF
Sbjct: 133 RNSTRRARDLESYDHEDYNIEYDYEQDSDDNSTTYS---FVKRCESINTVISFIWWIIGF 192

Query: 191 YWIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMA-FVGFFTVCCIIPFLAYGYTM 250
           YW+V GG  LL  +P LYWL+V+FLA DVFF +FC  +A  VG    CC+   +A  Y +
Sbjct: 193 YWVVEGGDKLLGEAPNLYWLSVIFLAIDVFFAVFCVVLACLVGIALCCCLPCIIALLYAV 252

Query: 251 TFLEGASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPG------YNG--YTTEHL 310
              EG SE E+  LP Y+F+      +F S++K+  G    PG       NG    TE  
Sbjct: 253 AGTEGVSEAELGVLPLYKFK------AFHSNEKNITG----PGKMVPIPINGLCLATERT 312

Query: 311 LNTEDSACCICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHG 344
           L  ED+ CCICL+ Y  G +L  LPC+HHFH+ CIVKWL++ ATCPLCK++I  G
Sbjct: 313 LLAEDADCCICLSSYEDGAELHALPCNHHFHSTCIVKWLKMRATCPLCKYNILKG 354

BLAST of Cp4.1LG10g10680 vs. ExPASy Swiss-Prot
Match: Q7T037 (E3 ubiquitin-protein ligase RNF12-B OS=Xenopus laevis OX=8355 GN=rnf12-b PE=2 SV=1)

HSP 1 Score: 68.9 bits (167), Expect = 1.3e-10
Identity = 37/110 (33.64%), Postives = 57/110 (51.82%), Query Frame = 0

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           G + +E  +LP     Q   L   D D+    G+T E   N  T  +  N     C +C+
Sbjct: 650 GVTLEESGSLPFLSLAQFFLLNEDDDDQPR--GLTKEQIDNLSTRNYGENDALKTCSVCI 709

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSI---GHGDSLV 348
            +Y  G +L  LPCSH +H  CI +WL  N+TCP+C+ ++   G+ +S+V
Sbjct: 710 TEYTEGNKLRKLPCSHEYHIHCIDRWLSENSTCPICRRAVLVAGNRESIV 757

BLAST of Cp4.1LG10g10680 vs. NCBI nr
Match: XP_023543467.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 712 bits (1839), Expect = 2.58e-259
Identity = 347/347 (100.00%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD
Sbjct: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE
Sbjct: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347

BLAST of Cp4.1LG10g10680 vs. NCBI nr
Match: XP_022949865.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata] >KAG6603427.1 E3 ubiquitin protein ligase RIE1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 712 bits (1838), Expect = 3.66e-259
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD
Sbjct: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE
Sbjct: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFS+GHGDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSVGHGDSLV 347

BLAST of Cp4.1LG10g10680 vs. NCBI nr
Match: XP_022967823.1 (E3 ubiquitin-protein ligase At1g63170-like [Cucurbita maxima])

HSP 1 Score: 705 bits (1820), Expect = 2.03e-256
Identity = 343/347 (98.85%), Postives = 344/347 (99.14%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MDGGRP STGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD
Sbjct: 1   MDGGRPLSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           V GGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE
Sbjct: 181 VMGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNPEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFS+GHGDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSVGHGDSLV 347

BLAST of Cp4.1LG10g10680 vs. NCBI nr
Match: XP_038881255.1 (E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida])

HSP 1 Score: 645 bits (1665), Expect = 8.59e-233
Identity = 311/347 (89.63%), Postives = 326/347 (93.95%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MD GRPSST FLSW+RT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDDEED+N YD
Sbjct: 1   MDDGRPSSTSFLSWQRTISSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDEEDDNEYD 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRV  RNGSSFIKPILVLDLFWNLFFVF+SVIVLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVF AY RRS+RYRF FENR AQDE RLSH+RIR +KRLEA NTMI+YIWWVFGFYWI
Sbjct: 121 VFFVFVAYLRRSNRYRFGFENRGAQDEFRLSHHRIRVMKRLEALNTMIAYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           V GGQ+LLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFF VCCI+PFLAYGYTMTFLE
Sbjct: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIVPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRF QDNPLESFDSDKK EVGMTLEPGY+G+TTEH LN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLESFDSDKKQEVGMTLEPGYSGHTTEHTLNPEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPC+HHFHTRCIVKWLRINATCPLCKFSIG GDSLV
Sbjct: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347

BLAST of Cp4.1LG10g10680 vs. NCBI nr
Match: XP_004144310.1 (E3 ubiquitin-protein ligase At4g11680 [Cucumis sativus] >KGN54665.1 hypothetical protein Csa_012005 [Cucumis sativus])

HSP 1 Score: 640 bits (1652), Expect = 8.23e-231
Identity = 308/347 (88.76%), Postives = 325/347 (93.66%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MD  RPS++GFLSW+RT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDD+ED+N Y+
Sbjct: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRV  RNGSSFIKPILVLDLFWNLFFVF+SVIVLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVF AY RRSSRYR  FENR AQDELRLSHNRIR +KRLEA NTM++YIWWVFGFYWI
Sbjct: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           V GGQ+LLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFF VCCIIPFLAYGYTM FLE
Sbjct: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRF QDNPLESFD+DKK EVGMTLEPGYNG+TTEH LN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPC+HHFHTRCIVKWLRINATCPLCKFSIG GDSLV
Sbjct: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347

BLAST of Cp4.1LG10g10680 vs. ExPASy TrEMBL
Match: A0A6J1GE01 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita moschata OX=3662 GN=LOC111453131 PE=4 SV=1)

HSP 1 Score: 712 bits (1838), Expect = 1.77e-259
Identity = 346/347 (99.71%), Postives = 347/347 (100.00%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD
Sbjct: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE
Sbjct: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFS+GHGDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSVGHGDSLV 347

BLAST of Cp4.1LG10g10680 vs. ExPASy TrEMBL
Match: A0A6J1HW81 (E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111467225 PE=4 SV=1)

HSP 1 Score: 705 bits (1820), Expect = 9.83e-257
Identity = 343/347 (98.85%), Postives = 344/347 (99.14%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MDGGRP STGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD
Sbjct: 1   MDGGRPLSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH
Sbjct: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI
Sbjct: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           V GGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE
Sbjct: 181 VMGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNPEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFS+GHGDSLV
Sbjct: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSVGHGDSLV 347

BLAST of Cp4.1LG10g10680 vs. ExPASy TrEMBL
Match: A0A0A0KYD1 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G418580 PE=4 SV=1)

HSP 1 Score: 640 bits (1652), Expect = 3.98e-231
Identity = 308/347 (88.76%), Postives = 325/347 (93.66%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MD  RPS++GFLSW+RT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDD+ED+N Y+
Sbjct: 1   MDDVRPSNSGFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDDEDDNEYE 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRV  RNGSSFIKPILVLDLFWNLFFVF+SVIVLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVIVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVF AY RRSSRYR  FENR AQDELRLSHNRIR +KRLEA NTM++YIWWVFGFYWI
Sbjct: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           V GGQ+LLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFF VCCIIPFLAYGYTM FLE
Sbjct: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFAVCCIIPFLAYGYTMNFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRF QDNPLESFD+DKK EVGMTLEPGYNG+TTEH LN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPC+HHFHTRCIVKWLRINATCPLCKFSIG GDSLV
Sbjct: 301 AQYVHGVQLCMLPCNHHFHTRCIVKWLRINATCPLCKFSIGQGDSLV 347

BLAST of Cp4.1LG10g10680 vs. ExPASy TrEMBL
Match: A0A6J1DJQ2 (E3 ubiquitin-protein ligase At4g11680-like OS=Momordica charantia OX=3673 GN=LOC111021746 PE=4 SV=1)

HSP 1 Score: 627 bits (1617), Expect = 8.59e-226
Identity = 300/347 (86.46%), Postives = 322/347 (92.80%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MD GRPSSTGF+SWRRT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDDE+DE  YD
Sbjct: 1   MDEGRPSSTGFMSWRRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDEDDETEYD 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRV  R+GSSFIKPILVLDLFWNLFFVFV+V+VLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVIFRHGSSFIKPILVLDLFWNLFFVFVAVLVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVFFAY RRSSRYRF FE+R AQDELR SHNRIR +KRLEA NTM++YIWWVFGFY I
Sbjct: 121 VFFVFFAYLRRSSRYRFGFEHRGAQDELRFSHNRIRAMKRLEALNTMVAYIWWVFGFYLI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           V GGQ+L+EGSPRLYWLAV+FLAFDVFFIIFCTGMAFVG F VCCIIPFLAYGYTMTFLE
Sbjct: 181 VMGGQALVEGSPRLYWLAVIFLAFDVFFIIFCTGMAFVGLFAVCCIIPFLAYGYTMTFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRF QDNPLE FDSDK  E+GMTLEPG+N +TTEH L+ EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLEPFDSDKNQEIGMTLEPGFNDHTTEHTLHPEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGDSLV 347
           AQYVHGVQLCMLPC+HHFHTRCIVKWLRINATCPLCKF+IG GD+LV
Sbjct: 301 AQYVHGVQLCMLPCTHHFHTRCIVKWLRINATCPLCKFTIGQGDTLV 347

BLAST of Cp4.1LG10g10680 vs. ExPASy TrEMBL
Match: A0A5A7SMU7 (E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold447G00840 PE=4 SV=1)

HSP 1 Score: 563 bits (1451), Expect = 1.24e-200
Identity = 276/315 (87.62%), Postives = 290/315 (92.06%), Query Frame = 0

Query: 1   MDGGRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRGVIFGDCAEPRVDDEEDENGYD 60
           MD  RPS++ FLSW+RT+SSRFALPLIQDAANLIMEHRGVIFGDCA+PRVDDEED+N YD
Sbjct: 1   MDDVRPSNSAFLSWQRTMSSRFALPLIQDAANLIMEHRGVIFGDCADPRVDDEEDDNEYD 60

Query: 61  RRVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLH 120
           RRV  RNGSSFIKPILVLDLFWNLFFVF+SV VLVLSAEEKP+APLR WLSGYAVQCL H
Sbjct: 61  RRVLFRNGSSFIKPILVLDLFWNLFFVFLSVTVLVLSAEEKPTAPLRFWLSGYAVQCLFH 120

Query: 121 VFFVFFAYFRRSSRYRFVFENRVAQDELRLSHNRIRFLKRLEASNTMISYIWWVFGFYWI 180
           VFFVF AY RRSSRYR  FENR AQDELRLSHNRIR +KRLEA NTM++YIWWVFGFYWI
Sbjct: 121 VFFVFVAYLRRSSRYRLGFENRGAQDELRLSHNRIRVMKRLEALNTMVAYIWWVFGFYWI 180

Query: 181 VTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIPFLAYGYTMTFLE 240
           V GGQ+LLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVG F VCCIIPFLAYGYTM FLE
Sbjct: 181 VMGGQALLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGLFAVCCIIPFLAYGYTMNFLE 240

Query: 241 GASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACCICL 300
           GASEDEIRALPKYRF QDNPLESFD+DKK EVGMTLEPGYNG+TTEH LN EDSACCICL
Sbjct: 241 GASEDEIRALPKYRFHQDNPLESFDNDKKQEVGMTLEPGYNGHTTEHTLNAEDSACCICL 300

Query: 301 AQYVHGVQLCMLPCS 315
           AQYVHGVQLCMLP S
Sbjct: 301 AQYVHGVQLCMLPSS 315

BLAST of Cp4.1LG10g10680 vs. TAIR 10
Match: AT1G68070.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 246.9 bits (629), Expect = 2.4e-65
Identity = 141/356 (39.61%), Postives = 206/356 (57.87%), Query Frame = 0

Query: 4   GRPSSTGFLSWRRTLSSRFALPLIQDAANLIMEHRG--VIFGDCAEPRVDDEEDENGYDR 63
           G  SST  L  R+  SS    P+I    N     RG  ++  + A   +++   + GY  
Sbjct: 9   GSDSSTPLLRSRQ--SSPRRQPVIAVLLNRASGRRGASMVVRETAAQELEERRADWGYS- 68

Query: 64  RVFLRNGSSFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHV 123
                      KP++ LD+ WN  FV V++++L++  EEKP+ P+R+W+ GYA+QCL+HV
Sbjct: 69  -----------KPVVALDMLWNTAFVLVAIVMLLVFKEEKPNVPIRIWICGYAIQCLVHV 128

Query: 124 FFVFFAYFRRSSRYR------FVFENRVAQDELRLSHNRIRFL--KRLEASNTMISYIWW 183
             V+  + +R++R R          N+ ++DE     N  RFL  K  E+ NT+IS++WW
Sbjct: 129 VLVWLEFRKRNARSRPGDLEAAQATNQDSEDE----DNDERFLSTKTCESMNTIISFVWW 188

Query: 184 VFGFYWIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMA-FVGFFTVCCIIPFLAY 243
           + GFYW+V+GG  LL+ +  LYWL  VFLAFDVFF IFC  +A  +G    CC+   +A 
Sbjct: 189 IVGFYWLVSGGDILLQNATHLYWLTFVFLAFDVFFAIFCVVLACLIGIALCCCLPCIIAL 248

Query: 244 GYTMTFLEGASEDEIRALPKYRFRQDNPLESFDSDKKHEVG----MTLEPGYNGYTTEHL 303
            Y +   EGASE ++  LPKYRF       + ++D+K   G    + ++        E +
Sbjct: 249 LYAVAGQEGASEADLSILPKYRF------HTMNNDEKQSDGGGKMIPVDAASENLGNERV 308

Query: 304 LNTEDSACCICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSIGHGD 345
           L  ED+ CCICL+ Y  G +L  LPC+HHFH+ CIVKWL++NATCPLCKF+I  G+
Sbjct: 309 LLPEDADCCICLSSYEDGAELVSLPCNHHFHSTCIVKWLKMNATCPLCKFNILKGN 340

BLAST of Cp4.1LG10g10680 vs. TAIR 10
Match: AT1G63170.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 239.6 bits (610), Expect = 3.8e-63
Identity = 132/302 (43.71%), Postives = 181/302 (59.93%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYF 129
           ++ KP++VLD  WNL FV V+  VLVLS++E P+ PLR+W+ GY +QC++H+  V   Y 
Sbjct: 69  AYSKPVVVLDFVWNLAFVVVATAVLVLSSDENPNMPLRVWIIGYGLQCMMHMVCVCVEYR 128

Query: 130 RRSSRYRFVFENRVA------------QDELRLSHN--------------RIRFLKRLEA 189
           RR+SR R     R +            ++ L LS N                 F K LE+
Sbjct: 129 RRNSRRRRDLSPRSSSSSSSSSSSMDEEEGLGLSRNSDERYLELGQLENENNSFAKHLES 188

Query: 190 SNTMISYIWWVFGFYWIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTV 249
           +NTMIS+IWWV GFYW+ +GGQ L +GSP+LYWL +VFL FDVFF++FC  +A V    V
Sbjct: 189 ANTMISFIWWVIGFYWVSSGGQELAQGSPQLYWLCIVFLGFDVFFVVFCVALACVIGIAV 248

Query: 250 CCIIP-FLAYGYTMTFLEGASEDEIRALPKYRFRQ--DNPLESFDSDKKH--EVGMTLEP 309
           CC +P  +A  Y +   EGAS+++I  L K++FR+  D    + D ++      G+  E 
Sbjct: 249 CCCLPCIIAVLYAVAEQEGASKEDIDQLTKFKFRKVGDTMKHTVDEEQGQGDSGGVMTEC 308

Query: 310 GYNGYTTEHLLNTEDSACCICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKF 341
           G +    EH L  ED+ CCICL+ Y    +L  LPC HHFH  C+ KWL INATCPLCK+
Sbjct: 309 GTDS-PVEHALPHEDAECCICLSAYEDETELRELPCGHHFHCGCVDKWLYINATCPLCKY 368

BLAST of Cp4.1LG10g10680 vs. TAIR 10
Match: AT4G11680.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 235.7 bits (600), Expect = 5.5e-62
Identity = 124/283 (43.82%), Postives = 172/283 (60.78%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYF 129
           ++ KP++ LD+ WNL FV + V VL+LS +EKP+ PLR+W+ GY +QC LH+  V   Y 
Sbjct: 104 AYSKPVVFLDILWNLAFVAIGVAVLILSRDEKPNMPLRVWVVGYGIQCWLHMACVCVEYR 163

Query: 130 RRSSRYR------FVFENRVAQDELRLSHNRIRF-----LKRLEASNTMISYIWWVFGFY 189
           RR  R            N  +Q  + L+    R       K LE++NTM S+IWW+ GFY
Sbjct: 164 RRRRRRHPEDGGGSGLTNSSSQQYVSLAQLEDRGETSNPAKHLESANTMFSFIWWIIGFY 223

Query: 190 WIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVGFFTVCCIIP-FLAYGYTMT 249
           W+  GGQ+L   SP+LYWL ++FL FDVFF++FC  +A V    VCC +P  +A  Y + 
Sbjct: 224 WVSAGGQTLSSDSPQLYWLCIIFLGFDVFFVVFCVALACVIGLAVCCCLPCIIAILYAVA 283

Query: 250 FLEGASEDEIRALPKYRFRQDNPLESFDSDKKHEVGMTLEPGYNGYTTEHLLNTEDSACC 309
             EGAS+++I  +PK+RF +   +E      +   G+  E G +    E  L+ ED+ CC
Sbjct: 284 DQEGASKNDIDQMPKFRFTKTGNVEKLSGKAR---GIMTECGTDS-PIERSLSPEDAECC 343

Query: 310 ICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLCKFSI 341
           ICL +Y  GV+L  LPC+HHFH  CI KWL IN+ CPLCKF+I
Sbjct: 344 ICLCEYEDGVELRELPCNHHFHCTCIDKWLHINSRCPLCKFNI 382

BLAST of Cp4.1LG10g10680 vs. TAIR 10
Match: AT1G12760.1 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.9e-60
Identity = 127/304 (41.78%), Postives = 174/304 (57.24%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYF 129
           ++ KP++VLD+ WNL FV V+  +LV+S +E P  PLR+WL GYA+QC+LH+  V   Y 
Sbjct: 95  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 154

Query: 130 RR---------------------SSRYRFVFENRVAQ------DELRLSH---NRIRFLK 189
           RR                     SS    + E  +         +L L H         K
Sbjct: 155 RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 214

Query: 190 RLEASNTMISYIWWVFGFYWIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVG 249
            LE++NTM S+IWW+ GFYW+  GGQ L + SPR+YWL++VFL FDVFF++FC  +A V 
Sbjct: 215 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 274

Query: 250 FFTVCCIIP-FLAYGYTMTFLEGASEDEIRALPKYRFRQDNPLESFDSDKKHEV--GMTL 309
              VCC +P  +A  Y +   EGAS+++I  L K++FR+        +D+      G+  
Sbjct: 275 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMT 334

Query: 310 EPGYNGYTTEHLLNTEDSACCICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLC 341
           E G +    EH L  ED+ CCICL+ Y  G +L  LPC HHFH  C+ KWL INATCPLC
Sbjct: 335 ECGTDS-PIEHTLLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 394

BLAST of Cp4.1LG10g10680 vs. TAIR 10
Match: AT1G12760.2 (Zinc finger, C3HC4 type (RING finger) family protein )

HSP 1 Score: 229.6 bits (584), Expect = 3.9e-60
Identity = 127/304 (41.78%), Postives = 174/304 (57.24%), Query Frame = 0

Query: 70  SFIKPILVLDLFWNLFFVFVSVIVLVLSAEEKPSAPLRLWLSGYAVQCLLHVFFVFFAYF 129
           ++ KP++VLD+ WNL FV V+  +LV+S +E P  PLR+WL GYA+QC+LH+  V   Y 
Sbjct: 24  AYSKPVVVLDIVWNLAFVSVATAILVMSRKEHPIMPLRVWLLGYALQCVLHMVCVCVEYR 83

Query: 130 RR---------------------SSRYRFVFENRVAQ------DELRLSH---NRIRFLK 189
           RR                     SS    + E  +         +L L H         K
Sbjct: 84  RRNRRRTNRTTTTTPPRSRSSSSSSSSSSLEEEALGSRRNSGVQDLSLGHLDTESSSVAK 143

Query: 190 RLEASNTMISYIWWVFGFYWIVTGGQSLLEGSPRLYWLAVVFLAFDVFFIIFCTGMAFVG 249
            LE++NTM S+IWW+ GFYW+  GGQ L + SPR+YWL++VFL FDVFF++FC  +A V 
Sbjct: 144 HLESANTMFSFIWWIIGFYWVSAGGQELAQESPRIYWLSIVFLGFDVFFVVFCVALACVI 203

Query: 250 FFTVCCIIP-FLAYGYTMTFLEGASEDEIRALPKYRFRQDNPLESFDSDKKHEV--GMTL 309
              VCC +P  +A  Y +   EGAS+++I  L K++FR+        +D+      G+  
Sbjct: 204 GIAVCCCLPCIIAVLYAVADQEGASKEDIEQLTKFKFRKLGDANKHTNDEAQGTTEGIMT 263

Query: 310 EPGYNGYTTEHLLNTEDSACCICLAQYVHGVQLCMLPCSHHFHTRCIVKWLRINATCPLC 341
           E G +    EH L  ED+ CCICL+ Y  G +L  LPC HHFH  C+ KWL INATCPLC
Sbjct: 264 ECGTDS-PIEHTLLQEDAECCICLSAYEDGTELRELPCGHHFHCSCVDKWLYINATCPLC 323

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LDB85.4e-6243.71E3 ubiquitin-protein ligase At1g63170 OS=Arabidopsis thaliana OX=3702 GN=At1g631... [more]
Q93Z927.7e-6143.82E3 ubiquitin-protein ligase At4g11680 OS=Arabidopsis thaliana OX=3702 GN=At4g116... [more]
Q9LN715.5e-5941.78E3 ubiquitin-protein ligase At1g12760 OS=Arabidopsis thaliana OX=3702 GN=At1g127... [more]
Q8GUU22.8e-5841.36E3 ubiquitin protein ligase RIE1 OS=Arabidopsis thaliana OX=3702 GN=RIE1 PE=2 SV... [more]
Q7T0371.3e-1033.64E3 ubiquitin-protein ligase RNF12-B OS=Xenopus laevis OX=8355 GN=rnf12-b PE=2 SV... [more]
Match NameE-valueIdentityDescription
XP_023543467.12.58e-259100.00E3 ubiquitin-protein ligase At1g63170-like [Cucurbita pepo subsp. pepo][more]
XP_022949865.13.66e-25999.71E3 ubiquitin-protein ligase At1g63170-like [Cucurbita moschata] >KAG6603427.1 E3... [more]
XP_022967823.12.03e-25698.85E3 ubiquitin-protein ligase At1g63170-like [Cucurbita maxima][more]
XP_038881255.18.59e-23389.63E3 ubiquitin-protein ligase At4g11680-like [Benincasa hispida][more]
XP_004144310.18.23e-23188.76E3 ubiquitin-protein ligase At4g11680 [Cucumis sativus] >KGN54665.1 hypothetical... [more]
Match NameE-valueIdentityDescription
A0A6J1GE011.77e-25999.71E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1HW819.83e-25798.85E3 ubiquitin-protein ligase At1g63170-like OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A0A0KYD13.98e-23188.76RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_4G418580 P... [more]
A0A6J1DJQ28.59e-22686.46E3 ubiquitin-protein ligase At4g11680-like OS=Momordica charantia OX=3673 GN=LOC... [more]
A0A5A7SMU71.24e-20087.62E3 ubiquitin-protein ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
AT1G68070.12.4e-6539.61Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G63170.13.8e-6343.71Zinc finger, C3HC4 type (RING finger) family protein [more]
AT4G11680.15.5e-6243.82Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.13.9e-6041.78Zinc finger, C3HC4 type (RING finger) family protein [more]
AT1G12760.23.9e-6041.78Zinc finger, C3HC4 type (RING finger) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 296..336
e-value: 6.5E-5
score: 32.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 295..337
e-value: 1.5E-10
score: 41.2
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 296..337
score: 12.25323
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 280..344
e-value: 1.3E-16
score: 61.5
NoneNo IPR availablePANTHERPTHR45977TARGET OF ERK KINASE MPK-1coord: 17..347
NoneNo IPR availablePANTHERPTHR45977:SF19RING-FINGER UBIQUITIN LIGASEcoord: 17..347
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 282..341

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG10g10680.1Cp4.1LG10g10680.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane