Cp4.1LG09g09870 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview
NameCp4.1LG09g09870
Typegene
OrganismCucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
DescriptionWD repeat-containing protein 74-like
LocationCp4.1LG09: 8665851 .. 8670309 (-)
RNA-Seq ExpressionCp4.1LG09g09870
SyntenyCp4.1LG09g09870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGTTCCTGAGTTCTGAGAAAGAATTTCCGAAGTTTTCTTCCCTTTTGATATATTGTATTCTTCATTGTTTAGTAGGGGCTATACTGACCTATTACTGGGTTGATGATGAAATGTCTGTTGATCGGCCGATTATGTCTTTCATTTCTTTCATCTATGGTCAATTCAAATTCTTCATCATTTTTAGTCACCAAATTACTTCCTTTTATTTTTTATTGCAGTTACTAGCTGTTGCTCGAAAAAATGGACTGGTAAGTTGCTTGTTCAGACTTCTAGTGTAGTGAATCCTGCTCACATTGATTGAACTTAAAAATTTAAATATACTAATGCTCTTTTTGTAAAATCATGTTCCTGCCGTATACTCCAGATTGAAGTTCTTAATCCATTGAATGGCAATCTTCATGTTGCAATTTCGGACAATACTGATACTTCTCCACCAAAAGATGATGCTATTATTGGAATGCATTTATTTTCAAAAGATGAATCGGCGTTGGCGTCTAGGTTATGGATCAATTTATAATTTATGAGCATGTTATTTTGTAATTTGATAAAAGTGCAAAAGGTATCATCGTGTTGTTAATGATGAAATATTAAAATTTTCTTAAACTAGCTGGCTACTTCAACAAGACATCTATGCTGAATTCCTCTTAGTTTGATCATTCATTTTTAGGCGTTGCACCTTGCTTTCGTGTACAAAAAAAGGAAATGCGAGCATGAGGGCAATTGATTTTTCTAGCTCATCTTCAGACGATATCTCTACCAATCTTGTAAAAACGTGGAAAGTATGTGGTTCAGGTGATGTTTTGTGCTCTAAAGTCGATGGAAGTGAAACCCATGCATTGTTTGGAGGGTGAGTTAAGATCATGATTGAATTTAAGTATTATCTATACATTGGCACTAACAACCTTTACCTTGTATGCGCAGGAAGGGTGTTGAAGTCAATACATGGAATCTGGAACAGTGTACTAAGATTTGGACAGCAAAAGCAGTATGCTTTCAAGTTCTCCTTTGCATGTTTGTACAAAGCGATCAACATATTTCTACAGAATGTTTTTTGATTTCCTTTTGAAACATTTTGTTTTATTCAGCCAAAGAAGAACAGTCTTGGTATTTTCACACCAACTTGCTTCACATCCGTGACATTTCTTAGTAAAGATGACCATCGTAAATTTGCAGCTGGTACCAACAACCATCAGGTAATTCATAACAGTTTCTCATGGTATTAGTAATACAAGTTACAGAATAGGAAATAATAACACAAGTTGTAGAATTAACGGGAGTTTTAGAGAATTGAATAAATAAGTGTCTGATGTGGTCTTGGTTGCTATTTAAGTTTAATAATACTTCATTTTGAGCCTTTCAGAAAATTATTGATATCTGATTATCGGTGAAACGTTACAACTCTTGTACAATGTAACCTCTTTATTTTTAGTTTACACATCCATTATAAAAACCATTTATTTTGATGTATGAATCATATTACCGCTCTTTACTTCTTAAAACTATTTGTTTTTATTATTATTTTATGGGGGTGGGGGATTGGTCTTCTTTCTCATTCTATAACTGGAGTTTCTGATTAACCAACTCAGGTTCGATTGTATGACATCTCTGCTCAGAAGAGACCTGTTATCTCGTTTGATTTTCGAGAGACTCCTATTAAAGCCTTGGCTGAAGATGTAGATGGTAACACAATATTTGTGGGGAATGCAACTGGTGATCTTGCATCCTTTGATATTCGCAATGGTAAAATTTGTTGTGCTTGAGACAATGTTCTTTCTGATTTAATATTCTGGGCGTTCTATATTTTCATACAGTACTCTTGTTTCAGAAAAGATTGGCTGTTTTACGGGTTGGATCCCAATTGATGAGTACTACCCCAATAGTTTATTTAAAAGTTCTCATTCCACTGCATATTTTAGCATGCTTGAGATGTAGGTGATACAGTCATGGTGTCTTAAATATATAAATTATGTATGAGAATGGTGTACCAAGAAGAGTAGTCTTGACTGACAGCTACCTTCATTAGAAAGGAGAAAACAAATATTTTTCTTTAGACCAGGAATTCCCAGTAGCGTTCAAACTTTTTATTTCACCTTTATTCTGTCTGTTGGGAGCTATTTGATTGTTCCTTGATTGTCGTGAGTTTTTATTGATTTTGTTGGACTATCTCTATGATCTGTAAATAGCAGTTGATTAAAGTTTTTATTAATGGAGTAAATTTATGTGCCTTATTTTCTTTGCTTGACTCGTTTCTCACAGGAAAGCTATTGGGTTGCTTCTTGGGGAAATGTTCTGGCAGCATAAGGTCCATCGCCAGGCATCCAGAGTTCCCGATCATTGCATCATGTGGTGGGATATATTTTCCAATATGACAATCATCTAGCATTTCTCTGTCTGCTATACACATGGAAATCTTGTATTTTTACTAGGATTGTGTCTCTTTTTACATGTTTTGAAATATTCATATAAAAAAAAATCCTCTGCACAACGAGCCATACTCGTCTTTTATGCTAAAAAACTAGCATTTTTAGTACTTAATGTAAGTGGGTTGGTTAGGAAGAAACAGAATAATTAGTGTCACTTCAAACCTACCTATAGGCAATTCATTAAGTTGTGTTGAATCCAGTGGATAATGGAAGGATTAAAGGAGCCAAGATACAGTCAATAATTTGAGTGGCATCAATTCCATGAATGTATCCGGACTGCACTAAATATTAACTTTTTCCTTTTCTTCTTAGCTAGATTTATAAACTCAGATATACAAAACTGAGAATCAGAATAAGAACATATCATTTTCTTCAATGTGGAATCTACTTATGAGTGATGTTGAAATCTTTTTGCAGGACTTGATAGTTATGTTCGCTTCTGGGATATAAAGACAAGGCAACTTCTGTCTGCGGTAAGTTTTCATAATTCTAGTAAATAATAGGTTGTTTAGACGCATCATTCAGAATGAATGATGTAGAAGCAAGTTTTTCAGAAAATACTGGAAAGGACATTTTAGAAGAAGATAATGGTAGTCAATCTTATGGACTAATTTGCACCATTGTTGTGATTTAATTTATTGACTCGGAGCTATGAAGAAAAAAAAAAAAAAAGGCCATTTAGAGACAAATATAAGGTCTGATGGTTTAAAAGTATAGTTTAGAATTATCTTCCTAGTTGTTTAGAAGTTGTGCTGTTTAGATATGCATCCTGTCGGTCGATCACCAACATCCAATTTTGCAGTTGTTTTCTACAGAAGGGAGAATAATGAAAGGAAAATTTACTGTTCGAGTACAAAATTCTAAGCATGATCACTGATCTACTTAATAGCTTCCTAGAAAATGTAATGGGACCCTATGCATTAAAGTGGTTATATTTGATGTAGCCTACTGGATAGTACTCTTACTAAACTGAAGCCGAGTATTTCCAGCACTATATTCACATAATTCAGCTGGTACTTGCGTAAATATATTGCATGTTTGTAAAATCAGGGAGTCTATTTAATAATATGACAGAAAGAGAGAAAAAAAATTGCGTTTGATGCATAAATGTAAGCTGGGACAATATATCATATGATTATATTTGTCTTCATTTGCTTGCAGGTATTCCTAAAGCAGCATCTTAATGGTGTTGTCTTCGATTCCCATTTTGTTGGAGAAGGTTAGATTTGCTGATCCACACTTATCAAATTAATGTTTCGTAACAAAATCATCTTTCTATGTTGAGCTTGTTTTTAGGTTGTGCTGCATTAGTGCATTTGATTGACTTCCAACATAACTCTCCTAGGCTCCAAATGACTGATTGAAAGCCTATGATCTGTTCATTTGTTCTTTATCTCATTCATATCTGTCTCAGCAGACACAACACACTCTGCAGTAGAGTCAATCCAACAGGACATAGAGATAGGCCAAACCGTTAGCGAGGAGGAACACACGCCTCAGAAAAGAAAAAAGGCATCGAAAGAAGACGGTGAAGGCAAAAAGAGGAAGGGTGCAAGCAAAGAAAATAAAAAAAGCAAAAAGAAGTTGCATGGCGAAACCGAAAGTAAGCAGAGGTAGGAGTTCAAAATCTGATATTCTTTGTTCTTGGCAAAGCAGAAAGGTTAGTTCATGTTCTGATCTATTCAGTCTGCAGAGACTGTGGGAGATTAGAGGGTAAATGATAAAAGTTATTTATCTCACCAATGTATTAAATTTTGGCCCATATCATGATTTTTATGCGGGATGAAGTAGGTATGATTCCATTCGAAGTAGTTCAAGCACGTTCGACTCAAGTATCCCGTGTCAAATTTTATAATACTAGTAGTTAGAAGTATTTGAATATGATACCTATAATGTTTTAGATTAGCCCTCCATGTTTGAACTTTGTAACTTATGAGTTCGTCAATGAACAATTCAGTAAGTTCTTGAAATATTGTTTTTACTTCCTCCACAAAGTAAAATATAACACCTATTTGTATCTGTGAGTGCAATG

mRNA sequence

ATGAAGTTCCTGAGTTCTGAGAAAGAATTTCCGAAGTTTTCTTCCCTTTTGATATATTGTATTCTTCATTGTTTAGTAGGGGCTATACTGACCTATTACTGGGTTGATGATGAAATGTCTGTTGATCGGCCGATTATGTCTTTCATTTCTTTCATCTATGGTCAATTCAAATTCTTCATCATTTTTAGTCACCAAATTACTTCCTTTTATTTTTTATTGCAGTTACTAGCTGTTGCTCGAAAAAATGGACTGATTGAAGTTCTTAATCCATTGAATGGCAATCTTCATGTTGCAATTTCGGACAATACTGATACTTCTCCACCAAAAGATGATGCTATTATTGGAATGCATTTATTTTCAAAAGATGAATCGGCGTTGGCGTCTAGGCGTTGCACCTTGCTTTCGTGTACAAAAAAAGGAAATGCGAGCATGAGGGCAATTGATTTTTCTAGCTCATCTTCAGACGATATCTCTACCAATCTTGTAAAAACGTGGAAAGTATGTGGTTCAGGTGATGTTTTGTGCTCTAAAGTCGATGGAAGTGAAACCCATGCATTGTTTGGAGGGAAGGGTGTTGAAGTCAATACATGGAATCTGGAACAGTGTACTAAGATTTGGACAGCAAAAGCACCAAAGAAGAACAGTCTTGGTATTTTCACACCAACTTGCTTCACATCCGTGACATTTCTTAGTAAAGATGACCATCGTAAATTTGCAGCTGGTACCAACAACCATCAGGTTCGATTGTATGACATCTCTGCTCAGAAGAGACCTGTTATCTCGTTTGATTTTCGAGAGACTCCTATTAAAGCCTTGGCTGAAGATGTAGATGGTAACACAATATTTGTGGGGAATGCAACTGGTGATCTTGCATCCTTTGATATTCGCAATGGAAAGCTATTGGGTTGCTTCTTGGGGAAATGTTCTGGCAGCATAAGGTCCATCGCCAGGCATCCAGAGTTCCCGATCATTGCATCATGTGGACTTGATAGTTATGTTCGCTTCTGGGATATAAAGACAAGGCAACTTCTGTCTGCGGTATTCCTAAAGCAGCATCTTAATGGTGTTGTCTTCGATTCCCATTTTGTTGGAGAAGACACAACACACTCTGCAGTAGAGTCAATCCAACAGGACATAGAGATAGGCCAAACCGTTAGCGAGGAGGAACACACGCCTCAGAAAAGAAAAAAGGCATCGAAAGAAGACGGTGAAGGCAAAAAGAGGAAGGGTGCAAGCAAAGAAAATAAAAAAAGCAAAAAGAAGTTGCATGGCGAAACCGAAAGTAAGCAGAGGTAGGAGTTCAAAATCTGATATTCTTTGTTCTTGGCAAAGCAGAAAGGTTAGTTCATGTTCTGATCTATTCAGTCTGCAGAGACTGTGGGAGATTAGAGGGTAAATGATAAAAGTTATTTATCTCACCAATGTATTAAATTTTGGCCCATATCATGATTTTTATGCGGGATGAAGTAGGTATGATTCCATTCGAAGTAGTTCAAGCACGTTCGACTCAAGTATCCCGTGTCAAATTTTATAATACTAGTAGTTAGAAGTATTTGAATATGATACCTATAATGTTTTAGATTAGCCCTCCATGTTTGAACTTTGTAACTTATGAGTTCGTCAATGAACAATTCAGTAAGTTCTTGAAATATTGTTTTTACTTCCTCCACAAAGTAAAATATAACACCTATTTGTATCTGTGAGTGCAATG

Coding sequence (CDS)

ATGAAGTTCCTGAGTTCTGAGAAAGAATTTCCGAAGTTTTCTTCCCTTTTGATATATTGTATTCTTCATTGTTTAGTAGGGGCTATACTGACCTATTACTGGGTTGATGATGAAATGTCTGTTGATCGGCCGATTATGTCTTTCATTTCTTTCATCTATGGTCAATTCAAATTCTTCATCATTTTTAGTCACCAAATTACTTCCTTTTATTTTTTATTGCAGTTACTAGCTGTTGCTCGAAAAAATGGACTGATTGAAGTTCTTAATCCATTGAATGGCAATCTTCATGTTGCAATTTCGGACAATACTGATACTTCTCCACCAAAAGATGATGCTATTATTGGAATGCATTTATTTTCAAAAGATGAATCGGCGTTGGCGTCTAGGCGTTGCACCTTGCTTTCGTGTACAAAAAAAGGAAATGCGAGCATGAGGGCAATTGATTTTTCTAGCTCATCTTCAGACGATATCTCTACCAATCTTGTAAAAACGTGGAAAGTATGTGGTTCAGGTGATGTTTTGTGCTCTAAAGTCGATGGAAGTGAAACCCATGCATTGTTTGGAGGGAAGGGTGTTGAAGTCAATACATGGAATCTGGAACAGTGTACTAAGATTTGGACAGCAAAAGCACCAAAGAAGAACAGTCTTGGTATTTTCACACCAACTTGCTTCACATCCGTGACATTTCTTAGTAAAGATGACCATCGTAAATTTGCAGCTGGTACCAACAACCATCAGGTTCGATTGTATGACATCTCTGCTCAGAAGAGACCTGTTATCTCGTTTGATTTTCGAGAGACTCCTATTAAAGCCTTGGCTGAAGATGTAGATGGTAACACAATATTTGTGGGGAATGCAACTGGTGATCTTGCATCCTTTGATATTCGCAATGGAAAGCTATTGGGTTGCTTCTTGGGGAAATGTTCTGGCAGCATAAGGTCCATCGCCAGGCATCCAGAGTTCCCGATCATTGCATCATGTGGACTTGATAGTTATGTTCGCTTCTGGGATATAAAGACAAGGCAACTTCTGTCTGCGGTATTCCTAAAGCAGCATCTTAATGGTGTTGTCTTCGATTCCCATTTTGTTGGAGAAGACACAACACACTCTGCAGTAGAGTCAATCCAACAGGACATAGAGATAGGCCAAACCGTTAGCGAGGAGGAACACACGCCTCAGAAAAGAAAAAAGGCATCGAAAGAAGACGGTGAAGGCAAAAAGAGGAAGGGTGCAAGCAAAGAAAATAAAAAAAGCAAAAAGAAGTTGCATGGCGAAACCGAAAGTAAGCAGAGGTAG

Protein sequence

MKFLSSEKEFPKFSSLLIYCILHCLVGAILTYYWVDDEMSVDRPIMSFISFIYGQFKFFIIFSHQITSFYFLLQLLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLLSCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEVNTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISAQKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRSIARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVESIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR
Homology
BLAST of Cp4.1LG09g09870 vs. ExPASy Swiss-Prot
Match: Q58D06 (WD repeat-containing protein 74 OS=Bos taurus OX=9913 GN=WDR74 PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 8.2e-20
Identity = 89/301 (29.57%), Postives = 144/301 (47.84%), Query Frame = 0

Query: 132 TLLSCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHAL-FGGK 191
           TL++C   G   +       +SSD +    V      G G V   + D +  H +  GGK
Sbjct: 99  TLITCVDSGILRVWTDKDKEASSDPVLELRV------GPG-VCRMRQDPARPHIVATGGK 158

Query: 192 GVEVNTWNLEQCTK-IWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRL 251
              +  W+L+   + ++ AK  + + L +  P     + FL   + +K    T  HQVR+
Sbjct: 159 ENALKVWDLQGSEEPLFRAKNVRNDWLDLRVPVWDQDIQFL--PESQKLVTCTGYHQVRV 218

Query: 252 YD-ISAQKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKC 311
           YD  S Q+RPV+   + E P+ A+    +GN++ VGN  G LA  D+R G+LLGC  G  
Sbjct: 219 YDPASPQRRPVLEATYGEYPLTAVTLTPEGNSVIVGNTHGQLAEIDLRQGRLLGCLKG-L 278

Query: 312 SGSIRSIARHPEFPIIASCGLDSYVRFWDIKT-RQLLSAVFLKQHLNGVVFDSHFVGEDT 371
           +GS+R +  HP  P++ASCGLD  +R   I+  R L   V+LK  LN ++      G D 
Sbjct: 279 AGSVRGLQCHPSKPLLASCGLDRVLRIHRIRNPRGLEHKVYLKSQLNCLLLS----GRDN 338

Query: 372 THSAVESIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETE 429
                +  Q+  ++    +E +      + A+K      ++   + + ++ KKK  G T 
Sbjct: 339 WEDEPQEPQEPNKVPSEDTETDELWASLEAAAKRKLPDWEQTQGALQARRRKKKRPGSTS 385

BLAST of Cp4.1LG09g09870 vs. ExPASy Swiss-Prot
Match: Q6RFH5 (WD repeat-containing protein 74 OS=Homo sapiens OX=9606 GN=WDR74 PE=1 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.1e-19
Identity = 91/305 (29.84%), Postives = 144/305 (47.21%), Query Frame = 0

Query: 126 LASRRCTLLSCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHA 185
           LA    TL++C   G   +       +SSD +    V      G G V   + D +  H 
Sbjct: 93  LAQADGTLITCVDSGILRVWHDKDKDTSSDPLLELRV------GPG-VCRMRQDPAHPHV 152

Query: 186 L-FGGKGVEVNTWNLEQCTK-IWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTN 245
           +  GGK   +  W+L+   + ++ AK  + + L +  P     + FL     +K    T 
Sbjct: 153 VATGGKENALKIWDLQGSEEPVFRAKNVRNDWLDLRVPIWDQDIQFL--PGSQKLVTCTG 212

Query: 246 NHQVRLYD-ISAQKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLG 305
            HQVR+YD  S Q+RPV+   + E P+ A+     GN++ VGN  G LA  D+R G+LLG
Sbjct: 213 YHQVRVYDPASPQRRPVLETTYGEYPLTAMTLTPGGNSVIVGNTHGQLAEIDLRQGRLLG 272

Query: 306 CFLGKCSGSIRSIARHPEFPIIASCGLDSYVRFWDIKT-RQLLSAVFLKQHLNGVVFDSH 365
           C  G  +GS+R +  HP  P++ASCGLD  +R   I+  R L   V+LK  LN ++    
Sbjct: 273 CLKG-LAGSVRGLQCHPSKPLLASCGLDRVLRIHRIQNPRGLEHKVYLKSQLNCLLLS-- 332

Query: 366 FVGEDTTHSAVESIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKK 425
             G D      +  Q+  ++    +E +      + A+K    G ++   + + ++ KKK
Sbjct: 333 --GRDNWEDEPQEPQEPNKVPLEDTETDELWASLEAAAKRKLSGLEQPQGALQTRRRKKK 383

Query: 426 LHGET 427
             G T
Sbjct: 393 RPGST 383

BLAST of Cp4.1LG09g09870 vs. ExPASy Swiss-Prot
Match: Q8VCG3 (WD repeat-containing protein 74 OS=Mus musculus OX=10090 GN=Wdr74 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.1e-19
Identity = 92/299 (30.77%), Postives = 141/299 (47.16%), Query Frame = 0

Query: 126 LASRRCTLLSCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHA 185
           LA    TL++C   G   +   +   +SSD +    V      G G V   + D + TH 
Sbjct: 93  LAQADGTLITCVDSGILRVWCENDKEASSDPLLELKV------GPG-VCRMRQDPTHTHV 152

Query: 186 LFG-GKGVEVNTWNLEQCTK-IWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTN 245
           +   GK   +  W+L+   + ++ AK  + + L +  P       FL     +K    T 
Sbjct: 153 VATCGKENALKVWDLQGSEEPVFRAKNVRNDWLDLRVPIWDQDTQFL--PGSQKLVTCTG 212

Query: 246 NHQVRLYD-ISAQKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLG 305
            HQVR+YD +S Q+RPV+   + E P+ A+    +GN++ VGN  G LA  D R G+LLG
Sbjct: 213 YHQVRVYDPVSPQRRPVLEATYGEYPLTAMTLTPEGNSVIVGNTHGQLAEIDFRQGRLLG 272

Query: 306 CFLGKCSGSIRSIARHPEFPIIASCGLDSYVRFWDIKT-RQLLSAVFLKQHLNGVVFDSH 365
           C  G  +GS+R +  HP  P++ASCGLD  +R   I+  R L   V+LK  LN ++    
Sbjct: 273 CLKG-LAGSVRGLQCHPSKPLLASCGLDRVLRIHRIRNPRGLEHKVYLKSQLNCLLLSGR 332

Query: 366 FVGEDTTHSAVESIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKK 421
              ED      E  Q   E  +T   +E        A ++  +  + +GA +  KK K+
Sbjct: 333 DNWEDEPQEPQEPNQVPSEDTET---DELWASLEAAAKRKLPDLDQTQGALQRRKKKKR 378

BLAST of Cp4.1LG09g09870 vs. ExPASy Swiss-Prot
Match: Q54FW9 (WD repeat-containing protein DDB_G0290555 OS=Dictyostelium discoideum OX=44689 GN=DDB_G0290555 PE=4 SV=1)

HSP 1 Score: 83.6 bits (205), Expect = 6.1e-15
Identity = 82/334 (24.55%), Postives = 163/334 (48.80%), Query Frame = 0

Query: 85  IEVLNPLNGN-LHVAISD-----NTDTSPPKDD-AIIGMHLFSKDESALASRRCTLLSCT 144
           I+ ++PLN + L VA+ +      T T+P     A + +   +K +S  A+   + +   
Sbjct: 81  IKAIHPLNNDKLLVALENGLVDVKTLTAPITTQLAPMNLPTLNKKKSPAAAAAASTVGKN 140

Query: 145 KKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEVNTW 204
            K    +     + S S +++TNL        SG  +    + S     FGGK V +  W
Sbjct: 141 NKSKQVIVEPVNTQSFSLNVATNL--------SGFAM----NPSNDKFAFGGKDVNLTIW 200

Query: 205 NLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISAQ-- 264
           +LE+  K ++AK  K + L +  P     V +++ D   K   G ++ +++ YD+ ++  
Sbjct: 201 DLEKQVKTYSAKF-KHDFLNLQEPVSINVVKYMNDD---KILIG-SDFRIKAYDLRSKTN 260

Query: 265 KRPVISFDFRETPIKALA-EDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 324
           +   +   F + PI+++   +   +  +  ++ G +  +D+R  + +G F    +GS++ 
Sbjct: 261 RSSFLDVSFSKHPIQSIQYTNQKEHYFYASDSIGKVFCYDVRTSRQVGSFKDS-AGSVKD 320

Query: 325 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVES 384
           IA HP  P++A+ GLD ++R +++  R++L  +FLKQ L+ V+F      E+ T+   E 
Sbjct: 321 IAIHPTLPLLATVGLDRHLRVYNLDNRKMLHKIFLKQRLSCVLFSK----EEPTN---EI 380

Query: 385 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKR 409
            Q++ EI + + E ++           +G  KK+
Sbjct: 381 AQEEEEIWKNLEENKNRINNDDNDDNNEGNDKKK 389

BLAST of Cp4.1LG09g09870 vs. ExPASy Swiss-Prot
Match: Q6BUJ2 (Ribosome biogenesis protein NSA1 OS=Debaryomyces hansenii (strain ATCC 36239 / CBS 767 / BCRC 21394 / JCM 1990 / NBRC 0083 / IGC 2968) OX=284592 GN=NSA1 PE=3 SV=1)

HSP 1 Score: 55.5 bits (132), Expect = 1.8e-06
Identity = 61/238 (25.63%), Postives = 106/238 (44.54%), Query Frame = 0

Query: 205 IWTAKAPKKNSLGIFTPTCFTSVTFLSK--DDHRKFAAGTNNHQVRLYDISAQKRPVISF 264
           I+TA+  K + L +  P   +S+ F  +   D  KF   T   QVR+YD +  KRP+  +
Sbjct: 193 IFTARNVKNDHLDLRPPIWISSILFFEEKPKDGYKFLTSTRYGQVRIYDTTHGKRPIQDY 252

Query: 265 DFRETPIKALAEDVDGNTIFVGNATGDLASFDIRN--------------------GKLLG 324
              E PI  L        + V +    +A + +                       KLLG
Sbjct: 253 KVCEKPIVTLNFADSEEEVIVSDTHNLVAKYSLAQIDSKAFKTHSASAGEITKPVSKLLG 312

Query: 325 CF-LGKCSGSIRSIARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGV-VFDS 384
            F  G  +G+I  +    +  II++ GLD Y+R +DI +R++L+ V+L   ++ V + DS
Sbjct: 313 KFSAGGNTGAIVGVNIFDD-EIISTGGLDRYLRTYDISSREILAKVYLGVQISDVLMLDS 372

Query: 385 HFVGEDTTHSAV---------ESIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRK 410
               E+   S +         +  ++ +E  +  S+EE    + ++ +K   E KKR+
Sbjct: 373 EDEEEEEPESEMGDKRKKNIEKRRRKALEAEENESDEEELWNQLEENNKTKIEKKKRR 429

BLAST of Cp4.1LG09g09870 vs. NCBI nr
Match: XP_023542679.1 (WD repeat-containing protein 74-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 702 bits (1812), Expect = 1.67e-252
Identity = 357/357 (100.00%), Postives = 357/357 (100.00%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVES 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVES
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVES 366

Query: 375 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR
Sbjct: 367 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 423

BLAST of Cp4.1LG09g09870 vs. NCBI nr
Match: XP_023542678.1 (WD repeat-containing protein 74-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 697 bits (1800), Expect = 1.17e-250
Identity = 357/358 (99.72%), Postives = 357/358 (99.72%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGE-DTTHSAVE 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGE DTTHSAVE
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEADTTHSAVE 366

Query: 375 SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR
Sbjct: 367 SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 424

BLAST of Cp4.1LG09g09870 vs. NCBI nr
Match: XP_022994307.1 (WD repeat-containing protein 74 isoform X2 [Cucurbita maxima])

HSP 1 Score: 682 bits (1759), Expect = 1.96e-244
Identity = 345/357 (96.64%), Postives = 352/357 (98.60%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLF+KDE ALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFAKDELALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMR+IDFSSSSS+DISTN+VKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRSIDFSSSSSEDISTNIVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGT++HQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTSSHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVES 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAV+S
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVDS 366

Query: 375 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           IQQDIEI QT SEEEHTPQKRKKASKEDGEGKKRKGASKENKK KKK HGETESKQR
Sbjct: 367 IQQDIEISQTFSEEEHTPQKRKKASKEDGEGKKRKGASKENKKCKKKSHGETESKQR 423

BLAST of Cp4.1LG09g09870 vs. NCBI nr
Match: XP_022955242.1 (WD repeat-containing protein 74-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 680 bits (1754), Expect = 1.13e-243
Identity = 345/357 (96.64%), Postives = 351/357 (98.32%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTD+SPPKDDAIIGMHLF KDE ALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDSSPPKDDAIIGMHLFEKDELALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMR+IDFSSSSS+DISTN VKTWKVC SGDV+CSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRSIDFSSSSSEDISTNPVKTWKVCSSGDVMCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGT++HQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTSSHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVES 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGED THSAVES
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDITHSAVES 366

Query: 375 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKK HGETESKQR
Sbjct: 367 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKSHGETESKQR 423

BLAST of Cp4.1LG09g09870 vs. NCBI nr
Match: XP_022994306.1 (WD repeat-containing protein 74 isoform X1 [Cucurbita maxima])

HSP 1 Score: 677 bits (1747), Expect = 1.37e-242
Identity = 345/358 (96.37%), Postives = 352/358 (98.32%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLF+KDE ALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFAKDELALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMR+IDFSSSSS+DISTN+VKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRSIDFSSSSSEDISTNIVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGT++HQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTSSHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGE-DTTHSAVE 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGE DTTHSAV+
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEADTTHSAVD 366

Query: 375 SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           SIQQDIEI QT SEEEHTPQKRKKASKEDGEGKKRKGASKENKK KKK HGETESKQR
Sbjct: 367 SIQQDIEISQTFSEEEHTPQKRKKASKEDGEGKKRKGASKENKKCKKKSHGETESKQR 424

BLAST of Cp4.1LG09g09870 vs. ExPASy TrEMBL
Match: A0A6J1JVF3 (WD repeat-containing protein 74 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111490075 PE=4 SV=1)

HSP 1 Score: 682 bits (1759), Expect = 9.51e-245
Identity = 345/357 (96.64%), Postives = 352/357 (98.60%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLF+KDE ALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFAKDELALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMR+IDFSSSSS+DISTN+VKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRSIDFSSSSSEDISTNIVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGT++HQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTSSHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVES 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAV+S
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVDS 366

Query: 375 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           IQQDIEI QT SEEEHTPQKRKKASKEDGEGKKRKGASKENKK KKK HGETESKQR
Sbjct: 367 IQQDIEISQTFSEEEHTPQKRKKASKEDGEGKKRKGASKENKKCKKKSHGETESKQR 423

BLAST of Cp4.1LG09g09870 vs. ExPASy TrEMBL
Match: A0A6J1GT09 (WD repeat-containing protein 74-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111457261 PE=4 SV=1)

HSP 1 Score: 680 bits (1754), Expect = 5.49e-244
Identity = 345/357 (96.64%), Postives = 351/357 (98.32%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTD+SPPKDDAIIGMHLF KDE ALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDSSPPKDDAIIGMHLFEKDELALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMR+IDFSSSSS+DISTN VKTWKVC SGDV+CSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRSIDFSSSSSEDISTNPVKTWKVCSSGDVMCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGT++HQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTSSHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVES 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGED THSAVES
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDITHSAVES 366

Query: 375 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKK HGETESKQR
Sbjct: 367 IQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKSHGETESKQR 423

BLAST of Cp4.1LG09g09870 vs. ExPASy TrEMBL
Match: A0A6J1JYR5 (WD repeat-containing protein 74 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111490075 PE=4 SV=1)

HSP 1 Score: 677 bits (1747), Expect = 6.62e-243
Identity = 345/358 (96.37%), Postives = 352/358 (98.32%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLF+KDE ALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFAKDELALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMR+IDFSSSSS+DISTN+VKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRSIDFSSSSSEDISTNIVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGT++HQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTSSHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGE-DTTHSAVE 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGE DTTHSAV+
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEADTTHSAVD 366

Query: 375 SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           SIQQDIEI QT SEEEHTPQKRKKASKEDGEGKKRKGASKENKK KKK HGETESKQR
Sbjct: 367 SIQQDIEISQTFSEEEHTPQKRKKASKEDGEGKKRKGASKENKKCKKKSHGETESKQR 424

BLAST of Cp4.1LG09g09870 vs. ExPASy TrEMBL
Match: A0A6J1GUM0 (WD repeat-containing protein 74-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111457261 PE=4 SV=1)

HSP 1 Score: 675 bits (1742), Expect = 3.82e-242
Identity = 345/358 (96.37%), Postives = 351/358 (98.04%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPKDDAIIGMHLFSKDESALASRRCTLL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTD+SPPKDDAIIGMHLF KDE ALASRRCTLL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDSSPPKDDAIIGMHLFEKDELALASRRCTLL 126

Query: 135 SCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVEV 194
           SCTKKGNASMR+IDFSSSSS+DISTN VKTWKVC SGDV+CSKVDGSETHALFGGKGVEV
Sbjct: 127 SCTKKGNASMRSIDFSSSSSEDISTNPVKTWKVCSSGDVMCSKVDGSETHALFGGKGVEV 186

Query: 195 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDISA 254
           NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGT++HQVRLYDISA
Sbjct: 187 NTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTSSHQVRLYDISA 246

Query: 255 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 314
           QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS
Sbjct: 247 QKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIRS 306

Query: 315 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGE-DTTHSAVE 374
           IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGE D THSAVE
Sbjct: 307 IARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEADITHSAVE 366

Query: 375 SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKLHGETESKQR 431
           SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKK HGETESKQR
Sbjct: 367 SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGASKENKKSKKKSHGETESKQR 424

BLAST of Cp4.1LG09g09870 vs. ExPASy TrEMBL
Match: A0A6J1EA63 (WD repeat-containing protein 74-like OS=Cucurbita moschata OX=3662 GN=LOC111431283 PE=4 SV=1)

HSP 1 Score: 612 bits (1577), Expect = 5.30e-217
Identity = 316/361 (87.53%), Postives = 332/361 (91.97%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPP-KDDAIIGMHLFSKDESALASRRCTL 134
           LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPP KD+AI+GMHL SKDES LASRRCTL
Sbjct: 67  LLAVARKNGLIEVLNPLNGNLHVAISDNTDTSPPPKDEAIVGMHLLSKDESELASRRCTL 126

Query: 135 LSCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVE 194
           LSCT KGNASMR I+FSSSSS+D STNL +TWK+C SGDV+CSKVDGSETHALFGGKGVE
Sbjct: 127 LSCTTKGNASMRTIEFSSSSSEDTSTNLAQTWKICSSGDVMCSKVDGSETHALFGGKGVE 186

Query: 195 VNTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDIS 254
           VN WNLEQCTKIWTAKAPKKNSLGIFTPT FTS TFLSKDDHRKFAAGTN+HQVRLYDIS
Sbjct: 187 VNMWNLEQCTKIWTAKAPKKNSLGIFTPTWFTSATFLSKDDHRKFAAGTNSHQVRLYDIS 246

Query: 255 AQKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIR 314
           AQKRPVISFDF ETPIKALAEDVDGNTIFVGNA+GDLASFDIRNGKLLGCFLGKCSGSIR
Sbjct: 247 AQKRPVISFDFGETPIKALAEDVDGNTIFVGNASGDLASFDIRNGKLLGCFLGKCSGSIR 306

Query: 315 SIARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDTTHSAVE 374
           SIARHPEF +IASCGLDSYVRFWDIKTRQLLSAVFLKQHL GVVFDSHFV ED THSAVE
Sbjct: 307 SIARHPEFSVIASCGLDSYVRFWDIKTRQLLSAVFLKQHLTGVVFDSHFVEEDVTHSAVE 366

Query: 375 SIQQDIEIGQTVSEEEHTPQKRKKASKEDGEGKKRKGAS---KENKKSKKKLHGETESKQ 431
            IQQ+ E+ QTV+EEEH PQKRKKA KEDGEG KRKG+    KENKKS++K H E ESKQ
Sbjct: 367 -IQQETEVTQTVNEEEHVPQKRKKAFKEDGEGGKRKGSKTIDKENKKSRRKSHREIESKQ 426

BLAST of Cp4.1LG09g09870 vs. TAIR 10
Match: AT1G29320.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.1e-107
Identity = 208/378 (55.03%), Postives = 262/378 (69.31%), Query Frame = 0

Query: 75  LLAVARKNGLIEVLNPLNGNLHVAIS-DNTDTSPPKDDAIIGMHLFSKDESALASRRCTL 134
           LLAVARK+G +EV+NP NG+LH + S    D   P+D+ I  +HLF K       R CTL
Sbjct: 67  LLAVARKDGNVEVINPCNGDLHFSYSVFGDDGCSPEDNEISALHLFRKKIDDQTERSCTL 126

Query: 135 LSCTKKGNASMRAIDFSSSSSDDISTNLVKTWKVCGSGDVLCSKVDGSETHALFGGKGVE 194
           L+CTKKG+ S+R++ F  +  +       KTWK CGSG++L  KVDGSE  +LFGGK VE
Sbjct: 127 LTCTKKGDVSLRSVKFPDAHGNSTDDASPKTWKACGSGEILVGKVDGSENFSLFGGKRVE 186

Query: 195 VNTWNLEQCTKIWTAKAPKKNSLGIFTPTCFTSVTFLSKDDHRKFAAGTNNHQVRLYDIS 254
            N W+LEQCTKIW+AK P KN+LGIFTPT FTS TFLSKDDHRKF  GT +HQVRLYDIS
Sbjct: 187 ANIWDLEQCTKIWSAKCPPKNNLGIFTPTWFTSATFLSKDDHRKFVTGTKSHQVRLYDIS 246

Query: 255 AQKRPVISFDFRETPIKALAEDVDGNTIFVGNATGDLASFDIRNGKLLGCFLGKCSGSIR 314
            Q+RPV+SFDFRET I ++AED DG+TI+VGNA+ DLASFDIR GKLLG FLGKCSGSIR
Sbjct: 247 TQRRPVLSFDFRETAITSIAEDPDGHTIYVGNASADLASFDIRTGKLLGSFLGKCSGSIR 306

Query: 315 SIARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFLKQHLNGVVFDSHFVGEDT----TH 374
           S+ RHP+  +IASCGLD Y+R +D+KTRQL+SAVFLKQHL G+VFDS F GE+T    T 
Sbjct: 307 SVVRHPQHQVIASCGLDRYLRVYDVKTRQLISAVFLKQHLTGLVFDSGFSGEETAVANTV 366

Query: 375 SAVESIQQDIEIGQTVSEEEHTPQKRKKASKE----------------DGEGKKRKGASK 432
           +   + ++   + Q   E E  P KRKK+ KE                + E +K    +K
Sbjct: 367 AEAATEEKMTIMDQEDDETEKAPVKRKKSKKEKRSREIVFEGEDDEENEDEIEKAPVKTK 426

BLAST of Cp4.1LG09g09870 vs. TAIR 10
Match: AT5G23430.1 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 3.4e-05
Identity = 31/127 (24.41%), Postives = 56/127 (44.09%), Query Frame = 0

Query: 230 LSKDDHRKFAAGTNNHQVRLYDISAQKRPVISFDFRETPIKALAEDVDGNTIFVGNATGD 289
           + +   R    G  +H+V L+ I  +   ++S     + I ++  D     +  G A+G 
Sbjct: 24  IGRKSSRVLVTGGEDHKVNLWAI-GKPNAILSLYGHSSGIDSVTFDASEVLVAAGAASGT 83

Query: 290 LASFDIRNGKLLGCFLGKCSGSIRSIARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFL 349
           +  +D+   K++    G  S  I S+  HP     AS  LD+ ++ WDI+ +  +     
Sbjct: 84  IKLWDLEEAKIVRTLTGHRSNCI-SVDFHPFGEFFASGSLDTNLKIWDIRKKGCIHT--Y 143

Query: 350 KQHLNGV 357
           K H  GV
Sbjct: 144 KGHTRGV 146

BLAST of Cp4.1LG09g09870 vs. TAIR 10
Match: AT5G23430.2 (Transducin/WD40 repeat-like superfamily protein )

HSP 1 Score: 47.4 bits (111), Expect = 3.4e-05
Identity = 31/127 (24.41%), Postives = 56/127 (44.09%), Query Frame = 0

Query: 230 LSKDDHRKFAAGTNNHQVRLYDISAQKRPVISFDFRETPIKALAEDVDGNTIFVGNATGD 289
           + +   R    G  +H+V L+ I  +   ++S     + I ++  D     +  G A+G 
Sbjct: 24  IGRKSSRVLVTGGEDHKVNLWAI-GKPNAILSLYGHSSGIDSVTFDASEVLVAAGAASGT 83

Query: 290 LASFDIRNGKLLGCFLGKCSGSIRSIARHPEFPIIASCGLDSYVRFWDIKTRQLLSAVFL 349
           +  +D+   K++    G  S  I S+  HP     AS  LD+ ++ WDI+ +  +     
Sbjct: 84  IKLWDLEEAKIVRTLTGHRSNCI-SVDFHPFGEFFASGSLDTNLKIWDIRKKGCIHT--Y 143

Query: 350 KQHLNGV 357
           K H  GV
Sbjct: 144 KGHTRGV 146

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q58D068.2e-2029.57WD repeat-containing protein 74 OS=Bos taurus OX=9913 GN=WDR74 PE=2 SV=1[more]
Q6RFH51.1e-1929.84WD repeat-containing protein 74 OS=Homo sapiens OX=9606 GN=WDR74 PE=1 SV=1[more]
Q8VCG31.1e-1930.77WD repeat-containing protein 74 OS=Mus musculus OX=10090 GN=Wdr74 PE=2 SV=1[more]
Q54FW96.1e-1524.55WD repeat-containing protein DDB_G0290555 OS=Dictyostelium discoideum OX=44689 G... [more]
Q6BUJ21.8e-0625.63Ribosome biogenesis protein NSA1 OS=Debaryomyces hansenii (strain ATCC 36239 / C... [more]
Match NameE-valueIdentityDescription
XP_023542679.11.67e-252100.00WD repeat-containing protein 74-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023542678.11.17e-25099.72WD repeat-containing protein 74-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_022994307.11.96e-24496.64WD repeat-containing protein 74 isoform X2 [Cucurbita maxima][more]
XP_022955242.11.13e-24396.64WD repeat-containing protein 74-like isoform X2 [Cucurbita moschata][more]
XP_022994306.11.37e-24296.37WD repeat-containing protein 74 isoform X1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A0A6J1JVF39.51e-24596.64WD repeat-containing protein 74 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GT095.49e-24496.64WD repeat-containing protein 74-like isoform X2 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1JYR56.62e-24396.37WD repeat-containing protein 74 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1GUM03.82e-24296.37WD repeat-containing protein 74-like isoform X1 OS=Cucurbita moschata OX=3662 GN... [more]
A0A6J1EA635.30e-21787.53WD repeat-containing protein 74-like OS=Cucurbita moschata OX=3662 GN=LOC1114312... [more]
Match NameE-valueIdentityDescription
AT1G29320.11.1e-10755.03Transducin/WD40 repeat-like superfamily protein [more]
AT5G23430.13.4e-0524.41Transducin/WD40 repeat-like superfamily protein [more]
AT5G23430.23.4e-0524.41Transducin/WD40 repeat-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001680WD40 repeatSMARTSM00320WD40_4coord: 297..337
e-value: 0.0086
score: 25.3
coord: 212..251
e-value: 32.0
score: 7.5
IPR001680WD40 repeatPROSITEPS50082WD_REPEATS_2coord: 305..346
score: 10.074014
IPR015943WD40/YVTN repeat-like-containing domain superfamilyGENE3D2.130.10.10coord: 62..419
e-value: 3.1E-21
score: 77.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 381..431
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 383..411
NoneNo IPR availablePANTHERPTHR16038:SF5BNAANNG21410D PROTEINcoord: 75..247
NoneNo IPR availablePANTHERPTHR16038:SF5BNAANNG21410D PROTEINcoord: 246..425
IPR037379WDR74/Nsa1PANTHERPTHR16038NOP SEVEN ASSOCIATED PROTEIN 1coord: 75..247
IPR037379WDR74/Nsa1PANTHERPTHR16038NOP SEVEN ASSOCIATED PROTEIN 1coord: 246..425
IPR019775WD40 repeat, conserved sitePROSITEPS00678WD_REPEATS_1coord: 324..338
IPR036322WD40-repeat-containing domain superfamilySUPERFAMILY50978WD40 repeat-likecoord: 63..361

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cp4.1LG09g09870.1Cp4.1LG09g09870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042273 ribosomal large subunit biogenesis
cellular_component GO:0005730 nucleolus
cellular_component GO:0030687 preribosome, large subunit precursor
molecular_function GO:0005515 protein binding