Cp4.1LG09g06850 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG09g06850
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	DNA-directed RNA polymerases II and IV subunit 5A-like
Location	Cp4.1LG09: 5989122 .. 5990882 (-)
RNA-Seq Expression	Cp4.1LG09g06850
Synteny	Cp4.1LG09g06850

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGTTATCAGAGGAAGAAATCACGAGACTCTTTAGAGTCCGAAAAACATTGATGCAAATGTTGAAAGATCGAGGCTACTATGTTGGAGATTTGGACATTGAGATGTCAAGAAATCAATTTAGGAATAAGTATGGTGATAACATGAAGAGAGAAGATCTTGTTATCAATAAAGCCATGAGAAATAATAGCTCTGATCAGGTAGAGTTTCATGCTATACAATGATAATATGAAGCGTTAGCGATCCTTTTATTTGTTCCTCTCTTGTTTATGGCTCGTTTCTATAGATATATATATTTTTTCCTGAAGAGCCGAAGGTCGGTGTCAAGACGATGAAAACATATACAAATCGTATGAAATCGGAGAATGTTTTTCGAGCTATATTGGTTGTTCAACAGAATTTGACCCCGTTTGCTCGTACTTGTATAAGCGAAATATCGTCTAAATTCCATTTGGAGGTTTTTCAGGTATTGTTTGAGACTAAGCTGCAAAAGTTTTGTGATTTTCATATCATTGCGCTTATAATTACCCTTTTCTACTTGTTGTTGAAGGAAGCTGAATTGATGGTTAATGTTAAAGATCATGTTCTTATTCCTGAGCATCAGCTGTTGACAAATGAAGAGAAGAAGACGTTGTTGGAGAGGTATACGGTGAAAGAAACGCAGGTGAGTATCGATTTTTTTCCCGAAAGGCACCGAAGTGAACTCATTAAAAAGTCTCTTGAAATGTATATTGTTTTTGTGAGATCCCACGTTCGTTGGGGAGGAGAATAAAACATTTTTTTTATAAGGGTGTGGAAACCTTTCCCTAGTAGACGTATTATAAAAACATTAAGAGGAAGCCCAAAACGGAAAAACTCAAAAAAGACAATACTGTTTGCGATGAGTTTGAGTTGTTACAAATGGTAGTAAGCAAGATTGAGTTGTTACAAATGGTAGTAGAGCAAGATACTGGGCGATGTGCCAGCGAGGAGGCTGAGCCCTGAAGGGAGTTGACACGAGGCGGTGTGTCAGCAAGGACACTCGGCCCTGAAGGAGGTGGACACGAGGCGGTGTGTCAGCAAGGACACTCGGCCCTGAAGGAGGTGGACACGAGGCGGTGTGTCAGCAAGGACACTCGGCCCTGAAGGAGGTGGACACGAGGCGGTGTGTCAGCAAGGACACTGGGTCCTGAAGGGGGGGGGGGTGGATTGGGGGTCCCACATCGATGGGAGAAGGGAACGAGTGTCAGTGAGGACTGTGCCCTAAAGGGAGGTAGATTGTGAGATCCCACATCAGTTGAGGAGGAAAACGAAGCATTTTTTATAAGGGTCATTCCTTAGAAGGGTCTTGAAACCTCTCCTTAGTTTTAAAAACTTTAAGGAGAAGCCCGAAACGAAAAAAAATCCAAAAAGGATAATATCTGCTAGTAGTGAGCTTGAACCGTTACAATTTTGTTCTAACATAATGTTGTGTTTGTTTATCTCAGCTACCTCGCATTCAGGTCACTGATCCAATTGCTCGATATTACGGGTTGAAGCGTGGACAAGTCTTGAAGATCATCCGACCAAGTGAGACGGCAGGAAGGTATGTAACTTATAGATATGTTGTTTGAGATTGCCCTAACTCGAAGTTGAAATCATGGTATGGTACATGTAAATGTGTGAGCACTTTGAGCAGCCATGCTCATCAAATAACTTTTGTTGCTTCCACATCTTTGTTCTTAGCTGCCAAAGCTTTTTGTTGGTTCCATTGTGATGACAACGTTAATTTGCTAGCTTAA

mRNA sequence

ATGGTGTTATCAGAGGAAGAAATCACGAGACTCTTTAGAGTCCGAAAAACATTGATGCAAATGTTGAAAGATCGAGGCTACTATGTTGGAGATTTGGACATTGAGATGTCAAGAAATCAATTTAGGAATAAGTATGGTGATAACATGAAGAGAGAAGATCTTGTTATCAATAAAGCCATGAGAAATAATAGCTCTGATCAGATATATATATTTTTTCCTGAAGAGCCGAAGGTCGGTGTCAAGACGATGAAAACATATACAAATCGTATGAAATCGGAGAATGTTTTTCGAGCTATATTGGTTGTTCAACAGAATTTGACCCCGTTTGCTCGTACTTGTATAAGCGAAATATCGTCTAAATTCCATTTGGAGGTTTTTCAGGAAGCTGAATTGATGGTTAATGTTAAAGATCATGTTCTTATTCCTGAGCATCAGCTGTTGACAAATGAAGAGAAGAAGACGTTGTTGGAGAGGTATACGGTGAAAGAAACGCAGGTGACTGCCAAAGCTTTTTGTTGGTTCCATTGTGATGACAACGTTAATTTGCTAGCTTAA

Coding sequence (CDS)

ATGGTGTTATCAGAGGAAGAAATCACGAGACTCTTTAGAGTCCGAAAAACATTGATGCAAATGTTGAAAGATCGAGGCTACTATGTTGGAGATTTGGACATTGAGATGTCAAGAAATCAATTTAGGAATAAGTATGGTGATAACATGAAGAGAGAAGATCTTGTTATCAATAAAGCCATGAGAAATAATAGCTCTGATCAGATATATATATTTTTTCCTGAAGAGCCGAAGGTCGGTGTCAAGACGATGAAAACATATACAAATCGTATGAAATCGGAGAATGTTTTTCGAGCTATATTGGTTGTTCAACAGAATTTGACCCCGTTTGCTCGTACTTGTATAAGCGAAATATCGTCTAAATTCCATTTGGAGGTTTTTCAGGAAGCTGAATTGATGGTTAATGTTAAAGATCATGTTCTTATTCCTGAGCATCAGCTGTTGACAAATGAAGAGAAGAAGACGTTGTTGGAGAGGTATACGGTGAAAGAAACGCAGGTGACTGCCAAAGCTTTTTGTTGGTTCCATTGTGATGACAACGTTAATTTGCTAGCTTAA

Protein sequence

MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAMRNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKFHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQVTAKAFCWFHCDDNVNLLA

Homology

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy Swiss-Prot
Match: O81098 (DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=3702 GN=NRPB5A PE=1 SV=1)

HSP 1 Score: 262.3 bits (669), Expect = 4.1e-69
Identity = 127/165 (76.97%), Postives = 152/165 (92.12%), Query Frame = 0


Query: 2   VLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAMR 61
           +L+EEE+ RL+R++KTLMQML+DRGY++ D ++ M++ QF  K+GDNMKREDLV  KA R
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 62  NNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 121
           N++SDQ+YIFFP+E KVGVKTMK YTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 122 HLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
           HLEVFQEAE++VN+K+HVL+PEHQ+LT EEKKTLLERYTVKETQ+
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQL 165

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy Swiss-Prot
Match: Q5R587 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=9601 GN=POLR2E PE=2 SV=1)

HSP 1 Score: 150.6 bits (379), Expect = 1.7e-35
Identity = 72/167 (43.11%), Postives = 116/167 (69.46%), Query Frame = 0


Query: 5   EEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGD-----NMKREDLVINKA 64
           EEE  RL+++RKT+MQ+  DRGY V   +++ +  +FR ++GD       +R DL +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFRAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  MRNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISS 124
             ++ +DQ+++FFPEEPKVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
           K+ LE F + EL++N+ +H L+PEH ++T EE   LL RY ++E Q+
Sbjct: 124 KYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQL 170

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy Swiss-Prot
Match: Q80UW8 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10090 GN=Polr2e PE=1 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.0e-35
Identity = 71/167 (42.51%), Postives = 117/167 (70.06%), Query Frame = 0


Query: 5   EEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGD-----NMKREDLVINKA 64
           EEE  RL+++RKT+MQ+  DRGY V   +++ +  +F+ ++GD       +R DL +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  MRNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISS 124
             ++ +DQ+++FFPEEPKVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
           K+ LE F + EL++N+ +H L+PEH ++T EE   LL RY ++E+Q+
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQL 170

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy Swiss-Prot
Match: B0BNE2 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus OX=10116 GN=Polr2e PE=2 SV=1)

HSP 1 Score: 149.8 bits (377), Expect = 3.0e-35
Identity = 71/167 (42.51%), Postives = 117/167 (70.06%), Query Frame = 0


Query: 5   EEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGD-----NMKREDLVINKA 64
           EEE  RL+++RKT+MQ+  DRGY V   +++ +  +F+ ++GD       +R DL +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQFGDKPSEGRPRRTDLTVLVA 63

Query: 65  MRNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISS 124
             ++ +DQ+++FFPEEPKVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
           K+ LE F + EL++N+ +H L+PEH ++T EE   LL RY ++E+Q+
Sbjct: 124 KYVLEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRESQL 170

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy Swiss-Prot
Match: P19388 (DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Homo sapiens OX=9606 GN=POLR2E PE=1 SV=4)

HSP 1 Score: 147.5 bits (371), Expect = 1.5e-34
Identity = 71/167 (42.51%), Postives = 115/167 (68.86%), Query Frame = 0


Query: 5   EEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGD-----NMKREDLVINKA 64
           EEE  RL+++RKT+MQ+  DRGY V   +++ +  +F+ + GD       +R DL +  A
Sbjct: 4   EEETYRLWKIRKTIMQLCHDRGYLVTQDELDQTLEEFKAQSGDKPSEGRPRRTDLTVLVA 63

Query: 65  MRNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISS 124
             ++ +DQ+++FFPEEPKVG+KT+K Y  RM+ EN+ RA++VVQQ +TP A+  + +++ 
Sbjct: 64  HNDDPTDQMFVFFPEEPKVGIKTIKVYCQRMQEENITRALIVVQQGMTPSAKQSLVDMAP 123

Query: 125 KFHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
           K+ LE F + EL++N+ +H L+PEH ++T EE   LL RY ++E Q+
Sbjct: 124 KYILEQFLQQELLINITEHELVPEHVVMTKEEVTELLARYKLRENQL 170

[Close]

BLAST of Cp4.1LG09g06850 vs. NCBI nr
Match: XP_023541037.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 325 bits (832), Expect = 6.31e-111
Identity = 165/166 (99.40%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM
Sbjct: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK
Sbjct: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. NCBI nr
Match: XP_022955185.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022994897.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima])

HSP 1 Score: 323 bits (828), Expect = 2.57e-110
Identity = 163/166 (98.19%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVLSEEEITRLFR+RKTLMQMLKDRGYYVGDLDI+MSRNQFRNKYGDNMKREDLVINKAM
Sbjct: 1   MVLSEEEITRLFRIRKTLMQMLKDRGYYVGDLDIDMSRNQFRNKYGDNMKREDLVINKAM 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK
Sbjct: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. NCBI nr
Match: XP_022959553.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959554.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_022959555.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_023004795.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004796.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023004797.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023513561.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo] >XP_023513563.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 298 bits (764), Expect = 1.44e-100
Identity = 145/166 (87.35%), Postives = 163/166 (98.19%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVL+EEEITRLFRVRKT++QMLKDRGY+VGD +++MSR QF+NKYG++MKRED+VINKA 
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RN+SSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEIS+K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAEL+VN+KDHVL+PEHQLL+NEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. NCBI nr
Match: XP_022143609.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Momordica charantia])

HSP 1 Score: 296 bits (759), Expect = 8.33e-100
Identity = 145/166 (87.35%), Postives = 161/166 (96.99%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVLSEEEITRLFR+RKT++QMLKDR Y+VGD +I+MSR QF++KYG+NMKRED+VINKA 
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKAR 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RN+SSDQIY+FFPEE KVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAEL+VN+KDHVL+PEHQLLTNEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. NCBI nr
Match: XP_022991826.1 (DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_023547519.1 DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 296 bits (758), Expect = 1.18e-99
Identity = 145/166 (87.35%), Postives = 162/166 (97.59%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVLSEEEITRLFR+RKT++QMLKDRGY+VGD +I+MSR QF++KYG++MKRED+VINKA 
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRGYFVGDFEIDMSREQFKHKYGESMKREDIVINKAK 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RN+SSDQIY+FFPEE KVGVKTMKTYTNRMK+ENVFRAILVVQQNLTPFARTCISEISSK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKTENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAEL+VNVKDHVL+PEHQLLTNEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELLVNVKDHVLVPEHQLLTNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy TrEMBL
Match: A0A6J1JX71 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111490485 PE=3 SV=1)

HSP 1 Score: 323 bits (828), Expect = 1.24e-110
Identity = 163/166 (98.19%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVLSEEEITRLFR+RKTLMQMLKDRGYYVGDLDI+MSRNQFRNKYGDNMKREDLVINKAM
Sbjct: 1   MVLSEEEITRLFRIRKTLMQMLKDRGYYVGDLDIDMSRNQFRNKYGDNMKREDLVINKAM 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK
Sbjct: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy TrEMBL
Match: A0A6J1GUG5 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=3662 GN=LOC111457227 PE=3 SV=1)

HSP 1 Score: 323 bits (828), Expect = 1.24e-110
Identity = 163/166 (98.19%), Postives = 166/166 (100.00%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVLSEEEITRLFR+RKTLMQMLKDRGYYVGDLDI+MSRNQFRNKYGDNMKREDLVINKAM
Sbjct: 1   MVLSEEEITRLFRIRKTLMQMLKDRGYYVGDLDIDMSRNQFRNKYGDNMKREDLVINKAM 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK
Sbjct: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy TrEMBL
Match: A0A6J1KT45 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=3661 GN=LOC111497988 PE=3 SV=1)

HSP 1 Score: 298 bits (764), Expect = 6.98e-101
Identity = 145/166 (87.35%), Postives = 163/166 (98.19%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVL+EEEITRLFRVRKT++QMLKDRGY+VGD +++MSR QF+NKYG++MKRED+VINKA 
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RN+SSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEIS+K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAEL+VN+KDHVL+PEHQLL+NEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy TrEMBL
Match: A0A6J1H699 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=3662 GN=LOC111460591 PE=3 SV=1)

HSP 1 Score: 298 bits (764), Expect = 6.98e-101
Identity = 145/166 (87.35%), Postives = 163/166 (98.19%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVL+EEEITRLFRVRKT++QMLKDRGY+VGD +++MSR QF+NKYG++MKRED+VINKA 
Sbjct: 1   MVLAEEEITRLFRVRKTVLQMLKDRGYFVGDFEVDMSREQFKNKYGESMKREDIVINKAK 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RN+SSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEIS+K
Sbjct: 61  RNDSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISTK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAEL+VN+KDHVL+PEHQLL+NEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLSNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. ExPASy TrEMBL
Match: A0A6J1CPU1 (DNA-directed RNA polymerases II and IV subunit 5A-like OS=Momordica charantia OX=3673 GN=LOC111013465 PE=3 SV=1)

HSP 1 Score: 296 bits (759), Expect = 4.03e-100
Identity = 145/166 (87.35%), Postives = 161/166 (96.99%), Query Frame = 0


Query: 1   MVLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAM 60
           MVLSEEEITRLFR+RKT++QMLKDR Y+VGD +I+MSR QF++KYG+NMKRED+VINKA 
Sbjct: 1   MVLSEEEITRLFRIRKTVLQMLKDRSYFVGDFEIDMSREQFKHKYGENMKREDIVINKAR 60

Query: 61  RNNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120
           RN+SSDQIY+FFPEE KVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK
Sbjct: 61  RNDSSDQIYVFFPEEQKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 120

Query: 121 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 166
           FHLEVFQEAEL+VN+KDHVL+PEHQLLTNEEKKTLLERYTVKETQ+
Sbjct: 121 FHLEVFQEAELLVNIKDHVLVPEHQLLTNEEKKTLLERYTVKETQL 166

[Close]

BLAST of Cp4.1LG09g06850 vs. TAIR 10
Match: AT3G22320.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 262.3 bits (669), Expect = 2.9e-70
Identity = 127/165 (76.97%), Postives = 152/165 (92.12%), Query Frame = 0


Query: 2   VLSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAMR 61
           +L+EEE+ RL+R++KTLMQML+DRGY++ D ++ M++ QF  K+GDNMKREDLV  KA R
Sbjct: 1   MLTEEELKRLYRIQKTLMQMLRDRGYFIADSELTMTKQQFIRKHGDNMKREDLVTLKAKR 60

Query: 62  NNSSDQIYIFFPEEPKVGVKTMKTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 121
           N++SDQ+YIFFP+E KVGVKTMK YTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF
Sbjct: 61  NDNSDQLYIFFPDEAKVGVKTMKMYTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 120

Query: 122 HLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
           HLEVFQEAE++VN+K+HVL+PEHQ+LT EEKKTLLERYTVKETQ+
Sbjct: 121 HLEVFQEAEMLVNIKEHVLVPEHQVLTTEEKKTLLERYTVKETQL 165

[Close]

BLAST of Cp4.1LG09g06850 vs. TAIR 10
Match: AT5G57980.1 (RNA polymerase II fifth largest subunit, C )

HSP 1 Score: 127.1 bits (318), Expect = 1.5e-29
Identity = 65/166 (39.16%), Postives = 110/166 (66.27%), Query Frame = 0


Query: 5   EEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNM---KREDLVINKAMR 64
           ++EITR+F+VR+T++QML+DRGY + + D+ + R +F  ++   M    +E L ++    
Sbjct: 5   DDEITRIFKVRRTVLQMLRDRGYTIEESDLNLKREEFVQRFCKTMNKVNKEALFVSANKG 64

Query: 65  NNSSDQIYIFFPEEPKVGVKTM-KTYTNRMKSENVFRAILVVQQNLTPFARTCISEISSK 124
            N +D+IY+F+PE PKVGV  + K    +M+ + V R I+VV   +T  AR  +SE++  
Sbjct: 65  PNPADKIYVFYPEGPKVGVPVIKKEVAIKMRDDKVHRGIVVVPMAITAPARMAVSELNKM 124

Query: 125 FHLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
             +EVF+EAEL+ N+ +H L+ ++ +L ++ KK LL  YTV++TQ+
Sbjct: 125 LTIEVFEEAELVTNITEHKLVNKYYVLDDQAKKKLLNTYTVQDTQL 170

[Close]

BLAST of Cp4.1LG09g06850 vs. TAIR 10
Match: AT3G57080.1 (Eukaryotic rpb5 RNA polymerase subunit family protein )

HSP 1 Score: 97.4 bits (241), Expect = 1.2e-20
Identity = 60/165 (36.36%), Postives = 98/165 (59.39%), Query Frame = 0


Query: 3   LSEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAMRN 62
           LS EE  R +  R+  +QML+DRGY V D DI +S + FR  YG+    + L I+   R+
Sbjct: 20  LSSEESHRYYLARRNGLQMLRDRGYEVSDEDINLSLHDFRTVYGERPDVDRLRISALHRS 79

Query: 63  NSSDQIYIFFPEEPKVGVKTMKT-YTNRMKSENVFRAILVVQQNLTPFARTCISEISSKF 122
           +S+ ++ I F     V V  +++   + +  E +   ILV+Q ++T  A   I   S  F
Sbjct: 80  DSTKKVKIVFFGTSMVKVNAIRSVVADILSQETITGLILVLQNHVTNQALKAIELFS--F 139

Query: 123 HLEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
            +E+FQ  +L+VN+  H L P+HQ+L +EEK TLL++++++E Q+
Sbjct: 140 KVEIFQITDLLVNITKHSLKPQHQVLNDEEKTTLLKKFSIEEKQL 182

[Close]

BLAST of Cp4.1LG09g06850 vs. TAIR 10
Match: AT2G41340.1 (RNA polymerase II fifth largest subunit, D )

HSP 1 Score: 95.9 bits (237), Expect = 3.6e-20
Identity = 60/164 (36.59%), Postives = 99/164 (60.37%), Query Frame = 0


Query: 4   SEEEITRLFRVRKTLMQMLKDRGYYVGDLDIEMSRNQFRNKYGDNMKREDLVINKAMRNN 63
           S  E  + +  R+T M+ML+DRGY V D DI +S  QFR  YG++   + L I+   R +
Sbjct: 17  SSVECHKYYLARRTTMEMLRDRGYDVSDEDINLSLQQFRALYGEHPDVDLLRISAKHRFD 76

Query: 64  SSDQIYIFFPEEPKVGVKTMKTY-TNRMKSENVFRAILVVQQNLTPFARTCISEISSKFH 123
           SS +I + F     V V  M+    + +  EN+   ILV+Q ++T  A   +   S  F 
Sbjct: 77  SSKKISVVFCGTGIVKVNAMRVIAADVLSRENITGLILVLQSHITNQALKAVELFS--FK 136

Query: 124 LEVFQEAELMVNVKDHVLIPEHQLLTNEEKKTLLERYTVKETQV 167
           +E+F+  +L+VNV  HVL P+HQ+L ++EK++LL++++++E Q+
Sbjct: 137 VELFEITDLLVNVSKHVLRPKHQVLNDKEKESLLKKFSIEEKQL 178

[Close]

BLAST of Cp4.1LG09g06850 vs. TAIR 10
Match: AT3G16680.1 (DNA binding;DNA-directed RNA polymerases )

HSP 1 Score: 81.3 bits (199), Expect = 9.2e-16
Identity = 44/79 (55.70%), Postives = 57/79 (72.15%), Query Frame = 0


Query: 83  MKTYTNRMKSENVFRAILVVQQNLTPFARTC---ISEISSKFHLEVFQEAELMVNVKDHV 142
           MK Y +++KS NVFRAILVV Q++  F+R     +  +   FH+EVFQE EL+VNVK+HV
Sbjct: 1   MKKYIDQLKSANVFRAILVV-QDIKAFSRQALVFLGAVYPIFHIEVFQEKELIVNVKEHV 60

Query: 143 LIPEHQLLTNEEKKTLLER 159
            +PEHQ LT EEK+  LER
Sbjct: 61  FVPEHQALTTEEKQKFLER 78

The following BLAST results are available for this feature:

BLAST of Cp4.1LG09g06850 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81098	4.1e-69	76.97	DNA-directed RNA polymerases II and IV subunit 5A OS=Arabidopsis thaliana OX=370...	[more]
Q5R587	1.7e-35	43.11	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Pongo abelii OX=96...	[more]
Q80UW8	3.0e-35	42.51	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Mus musculus OX=10...	[more]
B0BNE2	3.0e-35	42.51	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Rattus norvegicus ...	[more]
P19388	1.5e-34	42.51	DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Homo sapiens OX=96...	[more]

BLAST of Cp4.1LG09g06850 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023541037.1	6.31e-111	99.40	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita pepo subsp. pe...	[more]
XP_022955185.1	2.57e-110	98.19	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_...	[more]
XP_022959553.1	1.44e-100	87.35	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita moschata] >XP_...	[more]
XP_022143609.1	8.33e-100	87.35	DNA-directed RNA polymerases II and IV subunit 5A-like [Momordica charantia]	[more]
XP_022991826.1	1.18e-99	87.35	DNA-directed RNA polymerases II and IV subunit 5A-like [Cucurbita maxima] >XP_02...	[more]

BLAST of Cp4.1LG09g06850 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JX71	1.24e-110	98.19	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1GUG5	1.24e-110	98.19	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=...	[more]
A0A6J1KT45	6.98e-101	87.35	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita maxima OX=36...	[more]
A0A6J1H699	6.98e-101	87.35	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Cucurbita moschata OX=...	[more]
A0A6J1CPU1	4.03e-100	87.35	DNA-directed RNA polymerases II and IV subunit 5A-like OS=Momordica charantia OX...	[more]

BLAST of Cp4.1LG09g06850 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G22320.1	2.9e-70	76.97	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT5G57980.1	1.5e-29	39.16	RNA polymerase II fifth largest subunit, C	[more]
AT3G57080.1	1.2e-20	36.36	Eukaryotic rpb5 RNA polymerase subunit family protein	[more]
AT2G41340.1	3.6e-20	36.59	RNA polymerase II fifth largest subunit, D	[more]
AT3G16680.1	9.2e-16	55.70	DNA binding;DNA-directed RNA polymerases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	GENE3D	3.40.1340.10		coord: 3..130 e-value: 4.1E-47 score: 161.7
IPR036710	RNA polymerase Rpb5, N-terminal domain superfamily	SUPERFAMILY	53036	Eukaryotic RPB5 N-terminal domain	coord: 5..134
IPR005571	RNA polymerase, Rpb5, N-terminal	PFAM	PF03871	RNA_pol_Rpb5_N	coord: 5..90 e-value: 6.1E-32 score: 110.1
IPR000783	RNA polymerase, subunit H/Rpb5 C-terminal	PFAM	PF01191	RNA_pol_Rpb5_C	coord: 133..165 e-value: 4.6E-8 score: 32.8
IPR035913	RPB5-like RNA polymerase subunit superfamily	GENE3D	3.90.940.20		coord: 131..168 e-value: 3.5E-7 score: 31.8
IPR035913	RPB5-like RNA polymerase subunit superfamily	SUPERFAMILY	55287	RPB5-like RNA polymerase subunit	coord: 131..166
IPR014381	DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus	PIRSF	PIRSF000747	RPB5	coord: 1..167 e-value: 2.0E-60 score: 201.8
IPR039531	DNA-directed RNA polymerase subunit Rpb5-like	PANTHER	PTHR10535	DNA-DIRECTED RNA POLYMERASES I, II, AND III SUBUNIT RPABC1	coord: 1..167
None	No IPR available	PANTHER	PTHR10535:SF17	DNA-DIRECTED RNA POLYMERASES II AND IV SUBUNIT 5A-LIKE	coord: 1..167

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG09g06850.1	Cp4.1LG09g06850.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006351	transcription, DNA-templated
cellular_component	GO:0005736	RNA polymerase I complex
cellular_component	GO:0005665	RNA polymerase II, core complex
cellular_component	GO:0005666	RNA polymerase III complex
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003899	DNA-directed 5'-3' RNA polymerase activity