Cp4.1LG09g06560 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

BLAST of Cp4.1LG09g06560 vs. ExPASy Swiss-Prot
Match: Q8LBL1 (Two-pore potassium channel 1 OS=Arabidopsis thaliana OX=3702 GN=TPK1 PE=1 SV=2)

HSP 1 Score: 337.4 bits (864), Expect = 2.1e-91
Identity = 170/279 (60.93%), Postives = 222/279 (79.57%), Query Frame = 0

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           + R+V + LA YL  GTLCF+LV DQI+G KT+G+VDA+YFC+VTMTTVGYGDLVPNS  
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           ++LLACA+VF+G+ L G LLSRAADY+VEKQE  LV+A  + +     ++L+E   +KL+
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 194

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YK   T +VL VL +VGT+FL++VEK     AFYCVCST+TTLGYGDKSF++  GR+FAV
Sbjct: 195 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 254

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
            WI++S+ICLAQFFLY+AEL  E +Q++LV  VL+R +T +DLE ADLD D VV AAEF+
Sbjct: 255 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 314

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLV 385
           VYKLKEMGKI+++D+S I+D F +LD D+SG LT +D+V
Sbjct: 315 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of Cp4.1LG09g06560 vs. ExPASy Swiss-Prot
Match: Q850M0 (Two pore potassium channel a OS=Oryza sativa subsp. japonica OX=39947 GN=TPKA PE=1 SV=1)

HSP 1 Score: 305.8 bits (782), Expect = 6.8e-82
Identity = 174/345 (50.43%), Postives = 230/345 (66.67%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLE-HNNQPRNGDEVSYQRVVVKQ 105
           M+   ++QSLL     +   N LQR+ S     F R     +  P  G       V  K+
Sbjct: 1   MDDNSIQQSLL-----ADNPNVLQRKPSEGVNRFRRCRSTPSTDPLQGPPEKGSSVKAKE 60

Query: 106 V------SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGD 165
           +      SFR V ++L  YL  G L F+ V D+I+GK+TN ++DA+YFCVVTMTTVGYGD
Sbjct: 61  LFKEMRPSFRLVGLLLFIYLLVGVLAFYAVMDEISGKRTNRVLDALYFCVVTMTTVGYGD 120

Query: 166 LVPNSIGAKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRE 225
           LVPN+   KLLACA+VF G+ +  + +S+ ADY+VEKQE+   KA+  + K    ++LR 
Sbjct: 121 LVPNNDTTKLLACAFVFMGMAVVALFVSKVADYLVEKQEVLFFKALHTNLKGGETKMLRA 180

Query: 226 SVAHKLKYKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTT 285
              +++KYK     ++L + ++ GT+FL  VEK    D+FYCVC+TITTLGYGDKSFS+ 
Sbjct: 181 IETNRIKYKFYTNALLLVLSIISGTVFLWKVEKLSLVDSFYCVCATITTLGYGDKSFSSK 240

Query: 286 TGRVFAVIWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKV 345
            GRVFAV WI++STI +AQFF+YLAE+  ERRQK L N VL+R +T  DLE ADLD D+ 
Sbjct: 241 LGRVFAVFWIITSTIIMAQFFMYLAEIYTERRQKMLANWVLTRKMTKMDLEAADLDDDRQ 300

Query: 346 VSAAEFVVYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADL 384
           V AAEFVVYKLKE+GKINQE++S  L+ F KLD+D SG L+  DL
Sbjct: 301 VGAAEFVVYKLKELGKINQEEISSFLEEFEKLDVDHSGTLSPYDL 340

BLAST of Cp4.1LG09g06560 vs. ExPASy Swiss-Prot
Match: Q8LIN5 (Two pore potassium channel b OS=Oryza sativa subsp. japonica OX=39947 GN=TPKB PE=1 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 1.3e-72
Identity = 152/281 (54.09%), Postives = 191/281 (67.97%), Query Frame = 0

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFR V ++L  YL  GT+ F+L  D ++G +T   +DA+YFCVVTMTTVGYGDLVP S  
Sbjct: 64  SFRLVGLLLVAYLLLGTIAFYLAMDHMSGTRTTRALDALYFCVVTMTTVGYGDLVPASDA 123

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACA+VF G+ + G  LS+AADY+VEKQE  L +A      + S  ++R    +K++
Sbjct: 124 AKLLACAFVFAGVAVVGTFLSKAADYLVEKQEALLFRA------LHSHTMVRAMEMNKVR 183

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YK+   G++L   V  GT+ L  VE     DAFYCVC+T+TTLGYGD+SFS+  GR FAV
Sbjct: 184 YKLYTAGLLLVAAVASGTVVLWKVEGMRAVDAFYCVCATVTTLGYGDRSFSSEGGRAFAV 243

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
            WI  ST+ +A FFLY AEL  ERRQ+ L   VL R  T  DLE ADLD D  V AA+FV
Sbjct: 244 AWITVSTVVVALFFLYAAELYTERRQRELARWVLRRRTTNMDLEAADLDGDHRVGAADFV 303

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 387
           +YKLKE+GKI+QED+S  LD F  LD D SG L+ ADL  +
Sbjct: 304 LYKLKELGKISQEDISEFLDEFDNLDADHSGTLSPADLAAA 338

BLAST of Cp4.1LG09g06560 vs. ExPASy Swiss-Prot
Match: Q9FL25 (Two-pore potassium channel 2 OS=Arabidopsis thaliana OX=3702 GN=TPK2 PE=2 SV=1)

HSP 1 Score: 186.8 bits (473), Expect = 4.6e-46
Identity = 116/332 (34.94%), Postives = 191/332 (57.53%), Query Frame = 0

Query: 62  SKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQVSFRKVFVVLATYLGGG 121
           SK   +  R K+        ++ H N P+  D+ S  + +V Q        +L  YL  G
Sbjct: 107 SKPTINFHRSKTAPAMAAINNISHPNDPKT-DQQSDSKTIVNQ-----AVALLVVYLSLG 166

Query: 122 TLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIGAKLLACAYVFTGITLG 181
            L ++L  D  N K+T+ +VDA+YFC+VTM T+GYGD+ P+S+  KL +  +V  G    
Sbjct: 167 VLIYWLNRDSYNVKQTHPVVDALYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFM 226

Query: 182 GMLLSRAADYIVEKQEIHLVK-AIFMSKKISSGELLRESVAHKLKYKI---IMTGIVLWV 241
            +LLS    Y+++ QE ++++ A   S  ++  + +R  +    K ++   +  G+ L V
Sbjct: 227 DILLSGMVTYVLDLQENYMLETARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGV 286

Query: 242 LVV---VGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAVIWIMSSTIC 301
           +V+    G L +  VEK  + D+FY    ++TT+GYGD++F+T  GR+ A +W++ ST+ 
Sbjct: 287 VVLCLGFGVLIMHFVEKIGWLDSFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLA 346

Query: 302 LAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFVVYKLKEMGK 361
           +A+  L+LAE R ++R +    +VL   ++ S   +AD+D +  VS AEFV+YKLK+M K
Sbjct: 347 VARAILFLAESRVDKRNRERAKKVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDK 406

Query: 362 INQEDVSPILDTFYKLDIDQSGNLTEADLVTS 387
           I ++D++PI   F KLD   SG +T  DL+ S
Sbjct: 407 ITEKDINPIGFQFDKLDRTNSGRITLLDLLES 432

BLAST of Cp4.1LG09g06560 vs. ExPASy Swiss-Prot
Match: Q9SVV6 (Two-pore potassium channel 3 OS=Arabidopsis thaliana OX=3702 GN=TPK3 PE=2 SV=1)

HSP 1 Score: 182.2 bits (461), Expect = 1.1e-44
Identity = 115/339 (33.92%), Postives = 188/339 (55.46%), Query Frame = 0

Query: 52  EQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQVSFRKVF 111
           E  L  + S   + ++L R K+         L H  + ++  E S  R VV+Q      F
Sbjct: 102 EPWLSDQTSSHCQGHALHRSKTAPAMAVINDLHHPIRQKDPTETS--RSVVRQ-----AF 161

Query: 112 VVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIGAKLLAC 171
            +L  YL  G L ++L  D     +T+ +VD +YFC+VTM T+GYGD+ PNS+  KL + 
Sbjct: 162 ALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTKLFSI 221

Query: 172 AYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAH------KLK 231
            +V  G     +LLS    Y+++ QE +++ +   +K+    E  R  +        +++
Sbjct: 222 MFVLVGFGFIDILLSGMVSYVLDLQESYMLDS---AKRRDEPEKRRSYIIDVKKGRMRIR 281

Query: 232 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 291
            K+ +   V+ + + VG   +  +E+  + D+FY    ++TT+GYGD++F T  GR+FA 
Sbjct: 282 LKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRLFAA 341

Query: 292 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 351
           IW++ ST+ +A+ FLYLAE R ++R +    +VL   ++ S    AD+D++  VS AE+V
Sbjct: 342 IWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKAEYV 401

Query: 352 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLV 385
           +YKLKEM KI  +D+ PI   F KLD   +G +T  DL+
Sbjct: 402 IYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDLL 430

BLAST of Cp4.1LG09g06560 vs. NCBI nr
Match: KAG7012636.1 (Two-pore potassium channel 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 714 bits (1844), Expect = 6.41e-259
Identity = 369/376 (98.14%), Postives = 374/376 (99.47%), Query Frame = 0

Query: 11  MPSGEDLSRWKPESECWGKIIKLTAELMKIFVDFEMESGDVEQSLLPKLSDSKKRNSLQR 70
           MPSGEDLSRWKPESECWG+IIKLT+ELMKIFVDFEMESGDVEQSLLPKLSDSKKRNSLQR
Sbjct: 1   MPSGEDLSRWKPESECWGEIIKLTSELMKIFVDFEMESGDVEQSLLPKLSDSKKRNSLQR 60

Query: 71  RKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQVSFRKVFVVLATYLGGGTLCFFLVGD 130
           RKSIHHEIFSRSLEHNNQPRNGDE S+QRV+VKQVSFRKVFVVLA YLGGGTLCFFLVGD
Sbjct: 61  RKSIHHEIFSRSLEHNNQPRNGDETSHQRVIVKQVSFRKVFVVLAIYLGGGTLCFFLVGD 120

Query: 131 QINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIGAKLLACAYVFTGITLGGMLLSRAAD 190
           QINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIGAKLLACAYVFTGITLGGMLLSRAAD
Sbjct: 121 QINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIGAKLLACAYVFTGITLGGMLLSRAAD 180

Query: 191 YIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLKYKIIMTGIVLWVLVVVGTLFLILVE 250
           YIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLKYKIIMTGIVLWVLVVVGTLFLILVE
Sbjct: 181 YIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLKYKIIMTGIVLWVLVVVGTLFLILVE 240

Query: 251 KFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAVIWIMSSTICLAQFFLYLAELRAERR 310
           KFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAVIWIMSSTICLAQFFLYLAELRAERR
Sbjct: 241 KFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAVIWIMSSTICLAQFFLYLAELRAERR 300

Query: 311 QKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFVVYKLKEMGKINQEDVSPILDTFYKL 370
           QKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFVVYKLKEMGKINQED+SPILDTFYKL
Sbjct: 301 QKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFVVYKLKEMGKINQEDISPILDTFYKL 360

Query: 371 DIDQSGNLTEADLVTS 386
           DIDQSGNLTEADLVTS
Sbjct: 361 DIDQSGNLTEADLVTS 376

BLAST of Cp4.1LG09g06560 vs. NCBI nr
Match: XP_023542038.1 (two-pore potassium channel 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 650 bits (1678), Expect = 3.41e-234
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV
Sbjct: 1   MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG
Sbjct: 61  SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK
Sbjct: 121 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV
Sbjct: 181 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 386
           VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS
Sbjct: 301 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 341

BLAST of Cp4.1LG09g06560 vs. NCBI nr
Match: KAG6573519.1 (Two-pore potassium channel 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 644 bits (1661), Expect = 1.33e-231
Identity = 336/341 (98.53%), Postives = 339/341 (99.41%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDE S+QRV+VKQV
Sbjct: 1   MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDETSHQRVIVKQV 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFRKVFVVLA YLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG
Sbjct: 61  SFRKVFVVLAIYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK
Sbjct: 121 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV
Sbjct: 181 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 386
           VYKLKEMGKINQED+SPILDTFYKLDIDQSGNLTEADLVTS
Sbjct: 301 VYKLKEMGKINQEDISPILDTFYKLDIDQSGNLTEADLVTS 341

BLAST of Cp4.1LG09g06560 vs. NCBI nr
Match: XP_022925343.1 (two-pore potassium channel 1-like isoform X1 [Cucurbita moschata] >XP_022925344.1 two-pore potassium channel 1-like isoform X1 [Cucurbita moschata] >XP_022925345.1 two-pore potassium channel 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 638 bits (1645), Expect = 3.63e-229
Identity = 334/341 (97.95%), Postives = 337/341 (98.83%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           MESGDVEQSLLPKLSDSKK NSLQRRKSIHHEIFSRSLEHNNQPRNGDE S+QRV+VKQV
Sbjct: 1   MESGDVEQSLLPKLSDSKKSNSLQRRKSIHHEIFSRSLEHNNQPRNGDETSHQRVIVKQV 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFRKVFVVLA YLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG
Sbjct: 61  SFRKVFVVLAIYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACAYVFTGITLGGMLLSRAA+YIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK
Sbjct: 121 AKLLACAYVFTGITLGGMLLSRAANYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YKIIMTGIVLWVLVVVG LFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV
Sbjct: 181 YKIIMTGIVLWVLVVVGILFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 386
           VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS
Sbjct: 301 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 341

BLAST of Cp4.1LG09g06560 vs. NCBI nr
Match: XP_022967090.1 (two-pore potassium channel 1-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 637 bits (1643), Expect = 7.32e-229
Identity = 334/341 (97.95%), Postives = 338/341 (99.12%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           MESGDVEQSLLPKLSDS KRNSLQRRKSIHHEIFSRSLEHN+QPRNGDE S+QRVVVKQV
Sbjct: 1   MESGDVEQSLLPKLSDSNKRNSLQRRKSIHHEIFSRSLEHNDQPRNGDEASHQRVVVKQV 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG
Sbjct: 61  SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACAYVFTGITLGGMLLSRAADYIVEKQEI LVKAIFMSKKISSGELLRESVAHKLK
Sbjct: 121 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEILLVKAIFMSKKISSGELLRESVAHKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YKI+MTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV
Sbjct: 181 YKILMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLT+SDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTYSDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 386
           VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS
Sbjct: 301 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 341

BLAST of Cp4.1LG09g06560 vs. ExPASy TrEMBL
Match: A0A6J1EHP4 (two-pore potassium channel 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111432629 PE=3 SV=1)

HSP 1 Score: 638 bits (1645), Expect = 1.76e-229
Identity = 334/341 (97.95%), Postives = 337/341 (98.83%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           MESGDVEQSLLPKLSDSKK NSLQRRKSIHHEIFSRSLEHNNQPRNGDE S+QRV+VKQV
Sbjct: 1   MESGDVEQSLLPKLSDSKKSNSLQRRKSIHHEIFSRSLEHNNQPRNGDETSHQRVIVKQV 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFRKVFVVLA YLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG
Sbjct: 61  SFRKVFVVLAIYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACAYVFTGITLGGMLLSRAA+YIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK
Sbjct: 121 AKLLACAYVFTGITLGGMLLSRAANYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YKIIMTGIVLWVLVVVG LFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV
Sbjct: 181 YKIIMTGIVLWVLVVVGILFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 386
           VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS
Sbjct: 301 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 341

BLAST of Cp4.1LG09g06560 vs. ExPASy TrEMBL
Match: A0A6J1HTF8 (two-pore potassium channel 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466596 PE=3 SV=1)

HSP 1 Score: 637 bits (1643), Expect = 3.55e-229
Identity = 334/341 (97.95%), Postives = 338/341 (99.12%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           MESGDVEQSLLPKLSDS KRNSLQRRKSIHHEIFSRSLEHN+QPRNGDE S+QRVVVKQV
Sbjct: 1   MESGDVEQSLLPKLSDSNKRNSLQRRKSIHHEIFSRSLEHNDQPRNGDEASHQRVVVKQV 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG
Sbjct: 61  SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACAYVFTGITLGGMLLSRAADYIVEKQEI LVKAIFMSKKISSGELLRESVAHKLK
Sbjct: 121 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEILLVKAIFMSKKISSGELLRESVAHKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YKI+MTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV
Sbjct: 181 YKILMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLT+SDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTYSDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 386
           VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS
Sbjct: 301 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 341

BLAST of Cp4.1LG09g06560 vs. ExPASy TrEMBL
Match: A0A0A0LW31 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G173170 PE=3 SV=1)

HSP 1 Score: 525 bits (1351), Expect = 1.06e-184
Identity = 275/339 (81.12%), Postives = 302/339 (89.09%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           M+S D EQSLLPKLSDS K+NSL RRKS  H  FS S+E+NNQP+N D VS+QR+ V QV
Sbjct: 1   MDSDDFEQSLLPKLSDSDKKNSLLRRKSNRHGSFSHSVENNNQPQNYDVVSHQRIAVSQV 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFRKVFV+LATYLGGGT CFFLV DQI GKKTNG+VD+IYFCVVTMTTVGYGDLVP+S+ 
Sbjct: 61  SFRKVFVLLATYLGGGTFCFFLVRDQITGKKTNGVVDSIYFCVVTMTTVGYGDLVPDSMV 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLAC YVFTG+TLGGM+LS+AADYIVEKQEI LVKA+ M KKISS E+L+ES A+KLK
Sbjct: 121 AKLLACVYVFTGMTLGGMILSKAADYIVEKQEILLVKAMCMRKKISSSEILQESEANKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YK IMTGI+LW L+VVG LFL +VE  EFTDAFYCVCSTITTLGYGD+SFSTT GRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGILFLTVVENLEFTDAFYCVCSTITTLGYGDQSFSTTAGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWIMS TICLAQFFLYLAEL  ERRQ+SLVN VLSR LT+SDLEEADLDHDKVVSAAEFV
Sbjct: 241 IWIMSGTICLAQFFLYLAELYTERRQESLVNWVLSRSLTYSDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLV 384
           +YKLKEMGKINQEDVSPILDTF KLDIDQSG LTEAD+V
Sbjct: 301 IYKLKEMGKINQEDVSPILDTFKKLDIDQSGCLTEADIV 339

BLAST of Cp4.1LG09g06560 vs. ExPASy TrEMBL
Match: A0A6J1CMK6 (two-pore potassium channel 1-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111012651 PE=3 SV=1)

HSP 1 Score: 519 bits (1337), Expect = 1.38e-182
Identity = 270/341 (79.18%), Postives = 305/341 (89.44%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           M+ GDVEQSLLP L+DS KRN+LQRRKS     FS+ +E+NNQP+  DEVS+Q  + +Q+
Sbjct: 1   MDLGDVEQSLLPNLADSGKRNALQRRKSARRGSFSQLVENNNQPQICDEVSHQSTIAQQI 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFR+VFV+LATYLGGGTLCFFLV DQI+GKKTNGIVDA+YFCVVTMTTVGYGDLVP+S+ 
Sbjct: 61  SFRQVFVLLATYLGGGTLCFFLVQDQISGKKTNGIVDALYFCVVTMTTVGYGDLVPSSLV 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACAYVFTG+TLGGM+LS+AADYIVEKQEI LVK ++M   ISS E+++ES AHKLK
Sbjct: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKTMYMRNNISSSEIVQESEAHKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YK IMTGI+LW L+VVGTLFL  VEK EF DAFYCVCSTITTLGYGD+SFST+ GRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGTLFLTAVEKLEFMDAFYCVCSTITTLGYGDESFSTSAGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWI+SSTICLAQFFLYLAELR ERRQKSLVN VL+R+LT  DLEEADLDHDKVVSAAEFV
Sbjct: 241 IWILSSTICLAQFFLYLAELRTERRQKSLVNWVLNRNLTHLDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 386
           +YKLKEMGKI QED+SPILDTF KLDIDQSG+LTEADLVTS
Sbjct: 301 LYKLKEMGKICQEDISPILDTFKKLDIDQSGSLTEADLVTS 341

BLAST of Cp4.1LG09g06560 vs. ExPASy TrEMBL
Match: A0A6J1CLA7 (two-pore potassium channel 1-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111012651 PE=3 SV=1)

HSP 1 Score: 519 bits (1336), Expect = 1.95e-182
Identity = 270/341 (79.18%), Postives = 305/341 (89.44%), Query Frame = 0

Query: 46  MESGDVEQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQV 105
           M+ GDVEQSLLP L+DS KRN+LQRRKS     FS+ +E+NNQP+  DEVS+Q  + +Q+
Sbjct: 1   MDLGDVEQSLLPNLADSGKRNALQRRKSARSGSFSQLVENNNQPQICDEVSHQSTIAQQI 60

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           SFR+VFV+LATYLGGGTLCFFLV DQI+GKKTNGIVDA+YFCVVTMTTVGYGDLVP+S+ 
Sbjct: 61  SFRQVFVLLATYLGGGTLCFFLVQDQISGKKTNGIVDALYFCVVTMTTVGYGDLVPSSLV 120

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           AKLLACAYVFTG+TLGGM+LS+AADYIVEKQEI LVK ++M   ISS E+++ES AHKLK
Sbjct: 121 AKLLACAYVFTGMTLGGMILSKAADYIVEKQEILLVKTMYMRNNISSSEIVQESEAHKLK 180

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YK IMTGI+LW L+VVGTLFL  VEK EF DAFYCVCSTITTLGYGD+SFST+ GRVFAV
Sbjct: 181 YKFIMTGILLWALIVVGTLFLTAVEKLEFMDAFYCVCSTITTLGYGDESFSTSAGRVFAV 240

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
           IWI+SSTICLAQFFLYLAELR ERRQKSLVN VL+R+LT  DLEEADLDHDKVVSAAEFV
Sbjct: 241 IWILSSTICLAQFFLYLAELRTERRQKSLVNWVLNRNLTHLDLEEADLDHDKVVSAAEFV 300

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLVTS 386
           +YKLKEMGKI QED+SPILDTF KLDIDQSG+LTEADLVTS
Sbjct: 301 LYKLKEMGKICQEDISPILDTFKKLDIDQSGSLTEADLVTS 341

BLAST of Cp4.1LG09g06560 vs. TAIR 10
Match: AT5G55630.1 (Outward rectifying potassium channel protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.5e-92
Identity = 170/279 (60.93%), Postives = 222/279 (79.57%), Query Frame = 0

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           + R+V + LA YL  GTLCF+LV DQI+G KT+G+VDA+YFC+VTMTTVGYGDLVPNS  
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           ++LLACA+VF+G+ L G LLSRAADY+VEKQE  LV+A  + +     ++L+E   +KL+
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 194

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YK   T +VL VL +VGT+FL++VEK     AFYCVCST+TTLGYGDKSF++  GR+FAV
Sbjct: 195 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 254

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
            WI++S+ICLAQFFLY+AEL  E +Q++LV  VL+R +T +DLE ADLD D VV AAEF+
Sbjct: 255 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 314

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLV 385
           VYKLKEMGKI+++D+S I+D F +LD D+SG LT +D+V
Sbjct: 315 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of Cp4.1LG09g06560 vs. TAIR 10
Match: AT5G55630.2 (Outward rectifying potassium channel protein )

HSP 1 Score: 337.4 bits (864), Expect = 1.5e-92
Identity = 170/279 (60.93%), Postives = 222/279 (79.57%), Query Frame = 0

Query: 106 SFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIG 165
           + R+V + LA YL  GTLCF+LV DQI+G KT+G+VDA+YFC+VTMTTVGYGDLVPNS  
Sbjct: 75  NLRRVIMFLALYLTIGTLCFYLVRDQISGHKTSGVVDALYFCIVTMTTVGYGDLVPNSSA 134

Query: 166 AKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAHKLK 225
           ++LLACA+VF+G+ L G LLSRAADY+VEKQE  LV+A  + +     ++L+E   +KL+
Sbjct: 135 SRLLACAFVFSGMVLVGHLLSRAADYLVEKQEALLVRAFHLRQSFGPTDILKELHTNKLR 194

Query: 226 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 285
           YK   T +VL VL +VGT+FL++VEK     AFYCVCST+TTLGYGDKSF++  GR+FAV
Sbjct: 195 YKCYATCLVLVVLFIVGTIFLVMVEKMPVISAFYCVCSTVTTLGYGDKSFNSEAGRLFAV 254

Query: 286 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 345
            WI++S+ICLAQFFLY+AEL  E +Q++LV  VL+R +T +DLE ADLD D VV AAEF+
Sbjct: 255 FWILTSSICLAQFFLYVAELNTENKQRALVKWVLTRRITNNDLEAADLDEDGVVGAAEFI 314

Query: 346 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLV 385
           VYKLKEMGKI+++D+S I+D F +LD D+SG LT +D+V
Sbjct: 315 VYKLKEMGKIDEKDISGIMDEFEQLDYDESGTLTTSDIV 353

BLAST of Cp4.1LG09g06560 vs. TAIR 10
Match: AT5G46370.1 (Ca2+ activated outward rectifying K+ channel 2 )

HSP 1 Score: 186.8 bits (473), Expect = 3.3e-47
Identity = 116/332 (34.94%), Postives = 191/332 (57.53%), Query Frame = 0

Query: 62  SKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQVSFRKVFVVLATYLGGG 121
           SK   +  R K+        ++ H N P+  D+ S  + +V Q        +L  YL  G
Sbjct: 107 SKPTINFHRSKTAPAMAAINNISHPNDPKT-DQQSDSKTIVNQ-----AVALLVVYLSLG 166

Query: 122 TLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIGAKLLACAYVFTGITLG 181
            L ++L  D  N K+T+ +VDA+YFC+VTM T+GYGD+ P+S+  KL +  +V  G    
Sbjct: 167 VLIYWLNRDSYNVKQTHPVVDALYFCIVTMCTIGYGDITPDSVVTKLFSIFFVLVGFGFM 226

Query: 182 GMLLSRAADYIVEKQEIHLVK-AIFMSKKISSGELLRESVAHKLKYKI---IMTGIVLWV 241
            +LLS    Y+++ QE ++++ A   S  ++  + +R  +    K ++   +  G+ L V
Sbjct: 227 DILLSGMVTYVLDLQENYMLETARNESLNLNDRDKVRSYIIDVKKGRMRIRLKVGLALGV 286

Query: 242 LVV---VGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAVIWIMSSTIC 301
           +V+    G L +  VEK  + D+FY    ++TT+GYGD++F+T  GR+ A +W++ ST+ 
Sbjct: 287 VVLCLGFGVLIMHFVEKIGWLDSFYFSVMSVTTVGYGDRAFNTLAGRLLAAMWLLVSTLA 346

Query: 302 LAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFVVYKLKEMGK 361
           +A+  L+LAE R ++R +    +VL   ++ S   +AD+D +  VS AEFV+YKLK+M K
Sbjct: 347 VARAILFLAESRVDKRNRERAKKVLGESMSISQFLDADIDCNGCVSKAEFVIYKLKKMDK 406

Query: 362 INQEDVSPILDTFYKLDIDQSGNLTEADLVTS 387
           I ++D++PI   F KLD   SG +T  DL+ S
Sbjct: 407 ITEKDINPIGFQFDKLDRTNSGRITLLDLLES 432

BLAST of Cp4.1LG09g06560 vs. TAIR 10
Match: AT4G18160.1 (Ca2+ activated outward rectifying K+ channel 6 )

HSP 1 Score: 182.2 bits (461), Expect = 8.0e-46
Identity = 115/339 (33.92%), Postives = 188/339 (55.46%), Query Frame = 0

Query: 52  EQSLLPKLSDSKKRNSLQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQVSFRKVF 111
           E  L  + S   + ++L R K+         L H  + ++  E S  R VV+Q      F
Sbjct: 102 EPWLSDQTSSHCQGHALHRSKTAPAMAVINDLHHPIRQKDPTETS--RSVVRQ-----AF 161

Query: 112 VVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSIGAKLLAC 171
            +L  YL  G L ++L  D     +T+ +VD +YFC+VTM T+GYGD+ PNS+  KL + 
Sbjct: 162 ALLVVYLSLGVLIYWLNRDHYVVNQTHPVVDGLYFCIVTMCTIGYGDITPNSVVTKLFSI 221

Query: 172 AYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSKKISSGELLRESVAH------KLK 231
            +V  G     +LLS    Y+++ QE +++ +   +K+    E  R  +        +++
Sbjct: 222 MFVLVGFGFIDILLSGMVSYVLDLQESYMLDS---AKRRDEPEKRRSYIIDVKKGRMRIR 281

Query: 232 YKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKSFSTTTGRVFAV 291
            K+ +   V+ + + VG   +  +E+  + D+FY    ++TT+GYGD++F T  GR+FA 
Sbjct: 282 LKVALALGVVVLCIAVGVGIMHFIEEIGWLDSFYLSVMSVTTVGYGDRAFKTLPGRLFAA 341

Query: 292 IWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLDHDKVVSAAEFV 351
           IW++ ST+ +A+ FLYLAE R ++R +    +VL   ++ S    AD+D++  VS AE+V
Sbjct: 342 IWLLVSTLAVARAFLYLAEARVDKRNRERAKKVLCETMSVSQFFAADIDNNGCVSKAEYV 401

Query: 352 VYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLV 385
           +YKLKEM KI  +D+ PI   F KLD   +G +T  DL+
Sbjct: 402 IYKLKEMEKITDKDILPISKQFDKLDRCSNGKITLLDLL 430

BLAST of Cp4.1LG09g06560 vs. TAIR 10
Match: AT4G01840.1 (Ca2+ activated outward rectifying K+ channel 5 )

HSP 1 Score: 174.5 bits (441), Expect = 1.7e-43
Identity = 114/350 (32.57%), Postives = 192/350 (54.86%), Query Frame = 0

Query: 50  DVEQSLL----PKLSDSKKRNS-LQRRKSIHHEIFSRSLEHNNQPRNGDEVSYQRVVVKQ 109
           D+EQS+      + SDS + N  L + + +H    + ++      R     + +   V +
Sbjct: 55  DLEQSVQDDKEDQDSDSDETNRFLSQTRPLHRSRTAPAMVIIKDLRTKPPETKKPSPVSK 114

Query: 110 VSFRKVFVVLATYLGGGTLCFFLVGDQINGKKTNGIVDAIYFCVVTMTTVGYGDLVPNSI 169
              R+   +L  YL  G   +    D  +G +T+ +VDA+YFC+VTM T+GYGD+ P + 
Sbjct: 115 SIIRQAIFLLIVYLTLGVSIYSFNRDHYSGIETHPVVDALYFCIVTMCTIGYGDIAPLTP 174

Query: 170 GAKLLACAYVFTGITLGGMLLSRAADYIVEKQEIHLVKAIFMSK--------KISSGELL 229
             K+ A  +V  G     +LLS   +Y+++ QE  ++  I   +        + S+ + +
Sbjct: 175 WTKIFAVVFVLFGFGFLDILLSGVVNYVLDLQESMILTGIQTRQHHQHHHHHRFSAKDYI 234

Query: 230 --RESVAHKLKYKIIMTGIVLWVLVVVGTLFLILVEKFEFTDAFYCVCSTITTLGYGDKS 289
              E    +++ K+ +   V+ + + VG L L  VE+  F D+ Y    ++TT+GYGD++
Sbjct: 235 IDFEKGRMRIRMKVCLALCVVVLCIGVGALVLHFVEELGFVDSVYLSVMSVTTVGYGDRA 294

Query: 290 FSTTTGRVFAVIWIMSSTICLAQFFLYLAELRAERRQKSLVNRVLSRDLTFSDLEEADLD 349
           F T  GR+FA +W++ ST+ +A+ FLYLAE R +RR +  V   L+R++T  DL +AD  
Sbjct: 295 FKTLQGRLFAAVWLLVSTLAVARAFLYLAEARIDRRHRKAVKLALNREITVDDLLKADTY 354

Query: 350 HDKVVSAAEFVVYKLKEMGKINQEDVSPILDTFYKLDIDQSGNLTEADLV 385
               +S +E++V KLKEMGKI Q+D+  ++  F KLD +Q G +T  DL+
Sbjct: 355 QHGFISKSEYIVLKLKEMGKITQKDIDQVVIQFEKLDPNQIGKITLPDLL 404