Cp4.1LG04g01570 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG04g01570
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Calcium-binding EF hand family protein
Location	Cp4.1LG04: 514330 .. 518779 (+)
RNA-Seq Expression	Cp4.1LG04g01570
Synteny	Cp4.1LG04g01570

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCCGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTGGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCCAAAGAAAAGGCTTCCCCACAAGGCAATTCCTTAGAATCTGAGGCCACCCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGATGAGGAAATTGATGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCATGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATATGATCATAACAAAGATGGTAAAATTGATTTCAATGAGTTCGTTAAGTTTATGGAAATTGCCTTGTCTTGAATTTTTCAAATATGTTCAAAGGTGTTCCAATTTCAATTTCCATTTCCATTGTCATGTCCGACACTTTCATAGCCCTTTTGATGTCTAGGGGTTCTAATGTGTCGACATAAGAGATAAATTAATGTTTTCTATAACATTAAAAAGATGTGCAAAGTATGAGAAAAGTCAAAAAGATGTCACCAATGTCAACATAATATTAAAAATAAAAATTAATTATTGATATGGAACATTTTATCATGTTTTTAGTAAAGTAATAATTCTTCATGCATTCACATTTAATCTTGACCTGTACAAGTCATTAGTTGTTTGGAGATTTAAAACACACTTGACCTTGAGGAAGAACGTGGTGTTTGAGCAGTTCAATTTTATTAGACGAGACGTATTTGAGCTCTGGGTGAACTAACTTTTTTGTTCTTTAGACTTAGTTAACTTCCTTTTATCTTGTAGTGAAGGATCGATATCCTAGTCTGGAGGTGGGATCTCGTCGTATATCATAGAATAGTATGATGTCATCTTTTTAAGCATCTATGTATAATGTTAATAGGTGACCCTTATGTAAAAAACTATAAAGTCAAAAAGTTTGGGGGAGCGACATCATGTGCCACCCGCTACTGAGTCTACTTGTGGGGACTATTAGCAAGTTGTAATGCTCCATGCCTAGAATTCAAAATCTGGAATCGGTACCTAATGACCATGACATTTCTCTACATCTTCTGTGACATGGTCACGTTACTTACTTCTCGTTTCTAAGAGTGAAAACTATCCCCATAGATCAACACTGAAGTTTCTATGATTGAGCCAGTGAAAAGGAAAGTACGCCTTGTTGGTATAGGTAGTAATTTTAAATTCTTTTAAGTCTTTTTTAGTCATCCTACCCTTAGGGTCGCTCATGTGATTTCTATTTGTTTATATACCTCTCATTTACTTCAGTGTCACATGCCTACCAGTTTCCACCTTGGTTCGTCCTCGAGCCACATTTTACTAAGAGAGGTACTTCTCTAATACCATTTGTCATGGCCCACGCCCCCTGCATTCTCTGTGACATGGTTACATTACTTTTAGCTTTTAAGAGTGAAAACTATCCCTATAGACTTATATGGGTCCTTTCAGCATGTTTCGTGCTAGCTCACATGATTCTCAGAAAAATTTTCTGGAGGTCACCTAACTTAAAATTGCTTCAGGCAAAATACGCTTAACTTTTGAGTTCCTATGATTGAGACACTGAAAAAGAAGGTATCACATGACTATTGCATGCAAGTTAATCATTCACTCGTTGTTAACTATAGTTTGATCAAAAGTTTGTTTCCGTTGTAATAATTTTGTACCTTTAAGTAGTACTCATCTTCTAATGACCAATTTATTTATGATTGTATAGCTACCATATTTTCCTATTAAAAATAAGGTCGCTATTACATACATGACATGTCTAATATGCGTGAAATTTTTTATTTAAAATATTTCATAAACAATATTATGAACTTAAACGTTCCTAAATTCTATTTTAAATAAAACTAAACATCTATGGAATAACAAAAACAAAATAACATAATTTAGCGAGCAAGTCATCAAACCAAGTCAACAGCAAAACCACCCACTTACTTAATTGGCCTAAACCGAAGTCACTATATTCCTTTAGTTCTAGTTTAAAGTTGCTCATTGAATTCTAATTCTATTTAATTTAGAATTCAATTATAGAAATTGACTCATCTAATTGCATAAAATAATCAAAATTTGCATAAAATAATGGAAACTTTAGCTCTAATTACTTTAAATTTAGTTTAAAAAAAAACAGAAAATGCATTTTATAAAATTTATTATTTAAATACTTTGATCAAGGGATTCAAATAGTTTTGAAGTTTAATTTTTAGAAATTAAAGTCAAATCTATGAATTTTTTTTCACAACATAAGGGATAATTTTATATAATTAACATGGAGAGTCACAATCTCATGCCAAATTTTGTTATTTTAGTTTTCCACGTCAAATTGTCAGGATGGCCGAATGGTCTAAGACGCCAGAGGATTCAAATCCCACTTCTGACATTTTTATGTAGGTTTCTTTTATATAACAAATAAAACGTGCTTTTACATACTCTTAAAAACAATTATTCTAAAATTTTTAGGCATGGATGATAGTTTAGATTTGTTAAAAGCTCGATGTCCTTATTGTGAACCCGGAGTAAACAAGTGGCATATGAGTGAATCATGACCATATTTGAATGAAAGCAAATGTTGAATTCATCTAAATTTTGAATCTTAGACCTAGTGTGTTACCGTTATCATCTTTAAAAAAAAAGAAAAAGAAAAAATGATTTAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNCCATCTTTCCCCACTTCTCTCCACTCATCTCTCCTCCCTCCCCAATTTATCTCTCCTTAACCCATTGGCCAGAAAGTACAATTACTGTAAAAAGAAAAAGAAAAACAATAATGGTCCCATTTACTAATATGAGAATGACCTTTACCCAGTTGAGATTCACCCTAGATTTACATATGAAAATAGATCTGATCAATAAGATATATATTAAATCTTCATTTTCTTTTATTTTTTTTTAGAAATCACAACGAATTAGAGTTAGATCTTCATCACCCTAGATTTACATATGAAAATAGATCTGATCAATAAGATATATATTAAATCTTCATTTTCTTTTATTTTTTTTTAGAAATCACAACGAATTAGAGTTAGATCTTCATTGAATTATTACAATGATATAGCTTTGAGTCAGAAACTAAATGTCAATCATTTATACGATTCTAGTCCAATTAGTTAAATAGGAAACACTGAGATAAGGAAAGATTTGGAAACAGAGATCCACTCGAAATTTGGAATGCAACAGATAAAAAAAAATTAGGGAATTAAAAACCACAAACTAAATATAGAGGGAAAGGTAAACAACTGAGGGTAAATACAGAGAGTAGTAAAGCCCACTTGAAATAGCTGTATCTACTTTCTTATACAACAGTAACCATGAAAAATAAGATTTTTAATAAAAATATAATTATAGATATATAACATTTCAGACTCTATAACATTTCAAACTTTTAACATTGGACCAATGTAAATTAGAGAAATAATGTTGTAGAGTTCAAACCTGAAACTAGTGTCATGGGCTCATATAGGTTGACTCGAATTTCAAAAGACGGAATCGAACCTTAATTAAAGATATATTCGTGGCAACGACTTAAGCTAGCCAGGTAAGTGACCTTACTATCAGCATGATTATATTTTGATGTTGACACGTACCGTGTGGTTCTGCACGTGTCATATATATTGATATGTGTGAANCTGCACTGTAGGGAAAACGAAACGAAACGAAACGAAAGATAGGTCCCATCATTTCATTGCATGTGATTGTTGCATTTACCTCTAATAGTGGGGTCACTTACTGAGTATTTCTTTAAATATTCAAGNTTACGAGAAATGACATGAAATATAACCCCGTTAGATTTATGTATGATTTGAGAACGGAAGAATGAGAAACGATATGATATGAATGATGAACGATATCTGAAACGATATGAAAAATGATATGATATGTACTTAATGCTAGTGGTGTTCTATCAAATGATAATGAAATTGTAGAAGTAGTGGGGATCTCATGCATTATGTGTGTTCATGCATCTAGGGGGATACCCCTATCCCATCACGAGGGTACGGACGCGTACATCCAATGGCAGGACAATATGCTTTGCATAGTGCTGCCGTGACGGTTACTAGTTCTAACGGGTCCCGACATAAGGGGCTCCCAGAGTATGCCCACCGGATAAGGAAAGTGGCTCAGCGGTGTGCGAACTAG

mRNA sequence

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCCGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTGGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCCAAAGAAAAGGCTTCCCCACAAGGCAATTCCTTAGAATCTGAGGCCACCCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGATGAGGAAATTGATGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCATGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATATGATCATAACAAAGATGGTAAAATTGATTTCAATGAGTTCGGGGATACCCCTATCCCATCACGAGGGTACGGACGCGTACATCCAATGGCAGGACAATATGCTTTGCATAGTGCTGCCGTGACGGTTACTAGTTCTAACGGGTCCCGACATAAGGGGCTCCCAGAGTATGCCCACCGGATAAGGAAAGTGGCTCAGCGGTGTGCGAACTAG

Coding sequence (CDS)

ATGCCGAGTCAATTCCCCGTTGTCAACTTGAAGCTCGCCCCGACGCTCCTCCTCGTCGATCGGATATCGATTTCCTCGATTCTATTCTCCTTCGTGGATCGAATACATGACCTTATTTCTCAACCTTGGTCACTATTTCATTCACCCCCCAAAGAAAAGGCTTCCCCACAAGGCAATTCCTTAGAATCTGAGGCCACCCAACACAAAAACAGCTCAATCAGCAAAGAAGAAGTGAAATTTGTGATGGAAAAGCTTGAGTTTTTTTGCAGCGATGAGGAAATTGATGAATCGTGGCTTGGAGGTGAGGAAATAGGTGCCATTTTTTATGAGAGTGAGCCAAGCTTGGAGGAGTTGAAGCAGACATTTAATGTGTTCGATAAGAATAGAGATGGGTGTATTGATGCGGATGAGCTGCAGTCCATGCTGGGGTTGCTTGTACCTAATCAAGGCGTGTTCATCCATGATTGCCATAGAATGATTGCAAGATATGATCATAACAAAGATGGTAAAATTGATTTCAATGAGTTCGGGGATACCCCTATCCCATCACGAGGGTACGGACGCGTACATCCAATGGCAGGACAATATGCTTTGCATAGTGCTGCCGTGACGGTTACTAGTTCTAACGGGTCCCGACATAAGGGGCTCCCAGAGTATGCCCACCGGATAAGGAAAGTGGCTCAGCGGTGTGCGAACTAG

Protein sequence

MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNSLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQTFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEFGDTPIPSRGYGRVHPMAGQYALHSAAVTVTSSNGSRHKGLPEYAHRIRKVAQRCAN

Homology

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BLAST of Cp4.1LG04g01570 vs. ExPASy Swiss-Prot
Match: Q9MBG5 (Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 PE=2 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 6.8e-21
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQTFNVFDKNRDGC 132
           IS+EE + VM  L  F +D+++ E +   +E+ ++F E E SLEE+KQ F+VFD+N+DG 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQY-SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGF 142

Query: 133 IDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
           IDA ELQ +L +L   QG ++ +C  MI   D NKDGKIDFNEF
Sbjct: 143 IDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEF 185

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy Swiss-Prot
Match: Q93Z27 (Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 PE=1 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 1.5e-15
Identity = 46/108 (42.59%), Postives = 66/108 (61.11%), Query Frame = 0


Query: 70  NSSISKEEVKFVMEKLEFFCSDE-EIDESWLGGEEIGAIFYESEPSLEELKQTFNVFDKN 129
           +  + +E+V  VM+ L      E E  +     +E+  +F E EPSLEE+KQ F+VFD+N
Sbjct: 88  DDGLCREDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDEN 147

Query: 130 RDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
           RDG ID  +LQ +L +L   QG  + +C RMI  +D +KDG+IDF  F
Sbjct: 148 RDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGF 195

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 72.0 bits (175), Expect = 9.9e-12
Identity = 42/120 (35.00%), Postives = 68/120 (56.67%), Query Frame = 0


Query: 70  NSSISKEEVKFVMEKLEFFCSD-------EEID---ESWLGGEEIGAIF---YESEPSLE 129
           +  I+K+E+   +E L  +  D       E+ID   + ++  EE G ++    E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 130 ELKQTFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
           ++++ FNVFD+NRDG I  +EL+S+L  L   QG  + DC RMI++ D + DG ++F EF
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEF 137

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 65.5 bits (158), Expect = 9.3e-10
Identity = 42/117 (35.90%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 63  SEATQHKNSSISKEEVKFVMEKLEF---FCSDEEIDESWLGGEEIGAIFYESEPSLEELK 122
           SE  +     I  +E+  ++EK++     C D  IDE    GE    I  E +   E++K
Sbjct: 27  SETLRSLGIYIPDKELTQMIEKIDVNGDGCVD--IDEF---GELYKTIMDEEDEEEEDMK 86

Query: 123 QTFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
           + FNVFD+N DG I  DEL+++L  L   QG  + DC +MI + D + DG++++ EF
Sbjct: 87  EAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEF 138

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy Swiss-Prot
Match: Q9SN89 (Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 PE=2 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 2.7e-09
Identity = 49/150 (32.67%), Postives = 78/150 (52.00%), Query Frame = 0


Query: 30  SFVDRIHDLISQPWSLFHSPPKEKASPQGNSLESEATQHK-NSSISKEEVKFVMEKLE-- 89
           SF  R H   S  + LF S    K + +   ++     ++ +   S EEVK +++  E  
Sbjct: 34  SFPFRFHLFFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEAL 93

Query: 90  FFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQTFNVFDKNRDGCIDADELQSMLGLLV 149
           + C  EE +E  L   E+           E +K+ F +FD+N+DG ID +EL+ +L LL 
Sbjct: 94  YECLIEEGEEYLLEKNEM--------MGKEIVKEAFRLFDENQDGFIDENELKHVLSLLG 153

Query: 150 PNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
            ++   + +C +M+  YD N+DGKIDF EF
Sbjct: 154 YDECTKM-ECRKMVKVYDENRDGKIDFYEF 174

[Close]

BLAST of Cp4.1LG04g01570 vs. NCBI nr
Match: XP_023530007.1 (probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352 bits (904), Expect = 2.19e-121
Identity = 176/176 (100.00%), Postives = 176/176 (100.00%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176
           TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF
Sbjct: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176

[Close]

BLAST of Cp4.1LG04g01570 vs. NCBI nr
Match: XP_023003075.1 (probable calcium-binding protein CML30 [Cucurbita maxima])

HSP 1 Score: 346 bits (887), Expect = 8.53e-119
Identity = 171/176 (97.16%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFP+VNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEK SPQGNS
Sbjct: 1   MPSQFPIVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKVSPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEI+ESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176
           TFNVFDKNRDGCIDADELQS+LGLLVPNQGVFIHDCHRMIAR+DHNKDGKIDFNEF
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 176

[Close]

BLAST of Cp4.1LG04g01570 vs. NCBI nr
Match: XP_022927644.1 (probable calcium-binding protein CML30 [Cucurbita moschata])

HSP 1 Score: 344 bits (882), Expect = 4.93e-118
Identity = 172/176 (97.73%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLA TLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEI+ESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176
           TFNVFDKNRDGCIDADELQS+LGLLVPNQGVFIHDCHRMIAR+DHNKDGKIDFNEF
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 176

[Close]

BLAST of Cp4.1LG04g01570 vs. NCBI nr
Match: KAG6588366.1 (putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 342 bits (878), Expect = 2.00e-117
Identity = 171/176 (97.16%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCS+EEI+ESWLGGEEIGAIF ESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176
           TFNVFDKNRDGCIDADELQS+LGLLVPNQGVFIHDCHRMIAR+DHNKDGKIDFNEF
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 176

[Close]

BLAST of Cp4.1LG04g01570 vs. NCBI nr
Match: KAG7022215.1 (putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 340 bits (873), Expect = 1.16e-116
Identity = 170/176 (96.59%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHK+SSISKEEVKFVMEKLEFFCS+EEI+ESWLGGEEIGAIF ESEPSLEELKQ
Sbjct: 61  LESEATQHKSSSISKEEVKFVMEKLEFFCSEEEIEESWLGGEEIGAIFDESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176
           TFNVFDKNRDGCIDADELQS+LGLLVPNQGVFIHDCHRMIAR+DHNKDGKIDFNEF
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 176

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy TrEMBL
Match: A0A6J1KQS0 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111496787 PE=4 SV=1)

HSP 1 Score: 346 bits (887), Expect = 4.13e-119
Identity = 171/176 (97.16%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFP+VNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEK SPQGNS
Sbjct: 1   MPSQFPIVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKVSPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEI+ESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176
           TFNVFDKNRDGCIDADELQS+LGLLVPNQGVFIHDCHRMIAR+DHNKDGKIDFNEF
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 176

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy TrEMBL
Match: A0A6J1EIK8 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111434428 PE=4 SV=1)

HSP 1 Score: 344 bits (882), Expect = 2.38e-118
Identity = 172/176 (97.73%), Postives = 175/176 (99.43%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60
           MPSQFPVVNLKLA TLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS
Sbjct: 1   MPSQFPVVNLKLALTLLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEKASPQGNS 60

Query: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQ 120
           LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEI+ESWLGGEEIGAIFYESEPSLEELKQ
Sbjct: 61  LESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIEESWLGGEEIGAIFYESEPSLEELKQ 120

Query: 121 TFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176
           TFNVFDKNRDGCIDADELQS+LGLLVPNQGVFIHDCHRMIAR+DHNKDGKIDFNEF
Sbjct: 121 TFNVFDKNRDGCIDADELQSVLGLLVPNQGVFIHDCHRMIARFDHNKDGKIDFNEF 176

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy TrEMBL
Match: A0A6J1J5Q6 (probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC111481990 PE=4 SV=1)

HSP 1 Score: 177 bits (450), Expect = 1.13e-52
Identity = 102/187 (54.55%), Postives = 129/187 (68.98%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPT--------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKE 60
           M +  P +NL+L P+        L+ +DR SI      F D IHDL+S  +S F SPPKE
Sbjct: 1   MHNPQPTINLQLIPSTTTMMPPPLVFIDRASI------FADTIHDLVSLAFSFFQSPPKE 60

Query: 61  KASPQGNSLESEATQHKNSSISKEEVKFVMEKLEFFCS---DEEIDESWLGGEEIGAIFY 120
           KA+PQ  + +SE  +  +  +S+EEVK VMEKLE +C+   D+EIDE + G EE+  +F 
Sbjct: 61  KAAPQSRASQSEGKE--DWLMSREEVKSVMEKLELYCNEEGDDEIDECF-GREEVRGMFE 120

Query: 121 ESEPSLEELKQTFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDG 176
           E+EPSLEELKQTFNVFD+NRDG IDA EL ++LGLLV N  +FIHDC  MIAR+D N D 
Sbjct: 121 ENEPSLEELKQTFNVFDRNRDGFIDAHELHTVLGLLVSNNTIFIHDCKSMIARFDRNNDE 178

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy TrEMBL
Match: A0A6J1F854 (probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC111441729 PE=4 SV=1)

HSP 1 Score: 173 bits (439), Expect = 5.01e-51
Identity = 97/185 (52.43%), Postives = 126/185 (68.11%), Query Frame = 0


Query: 1   MPSQFPVVNLKLAPT-------LLLVDRISISSILFSFVDRIHDLISQPWSLFHSPPKEK 60
           M +  P +NL+L P+       L+ +DR SI      F   IHDL+S  +S F SPPK+K
Sbjct: 1   MHNPQPTINLQLIPSTTTTTPPLVFIDRASI------FAGTIHDLVSLAFSFFQSPPKDK 60

Query: 61  ASPQGNSLESEATQHKNSSISKEEVKFVMEKLEFFCSDEEIDE--SWLGGEEIGAIFYES 120
           A+PQ  + +SE+ +  +  +S+EEVK VMEKLE +C++EE DE     G +E+  +F E+
Sbjct: 61  AAPQSRASQSESKE--DWLMSREEVKSVMEKLELYCNEEEDDEIDECFGRDEVRGMFEEN 120

Query: 121 EPSLEELKQTFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKI 176
           EPSLEELKQTFNVFD+NRDG IDA EL ++LGLL  N  +FIHDC  MIAR+D N D KI
Sbjct: 121 EPSLEELKQTFNVFDRNRDGFIDAQELHTVLGLLASNNTIFIHDCKSMIARFDRNNDQKI 177

[Close]

BLAST of Cp4.1LG04g01570 vs. ExPASy TrEMBL
Match: A0A0A0LHQ5 (Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G825010 PE=4 SV=1)

HSP 1 Score: 152 bits (384), Expect = 2.02e-42
Identity = 94/173 (54.34%), Postives = 110/173 (63.58%), Query Frame = 0


Query: 19  VDRISISSILFSFVDRIHDLI-SQPWSLFHSPPKEKASPQGNSLESEA------TQHKNS 78
           VDRISI  ILF+F DRI +L+ S PW +     KEKA  Q N            TQ   S
Sbjct: 32  VDRISILPILFNFADRIQELVRSLPWPILQPHSKEKALSQCNCSSKSGDQIIITTQQNCS 91

Query: 79  SISKEEVKFVMEKLEFFCSDEE--------IDESWLGGEEIGAIFYESEPSLEELKQTFN 138
           S+S+EEV+FVMEKLE F  +E         I ES    E I  +F E+EPSLEELKQTFN
Sbjct: 92  SMSREEVRFVMEKLELFWREERDGGGGDKGIGES---DEMIRGMFEENEPSLEELKQTFN 151

Query: 139 VFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 176
           VFD+NRDG ID  EL  +L LL  N+G+FIHDC  MIAR+D N DGKIDF+EF
Sbjct: 152 VFDRNRDGFIDEHELFIVLSLLESNKGIFIHDCKTMIARFDLNNDGKIDFHEF 201

[Close]

BLAST of Cp4.1LG04g01570 vs. TAIR 10
Match: AT3G29000.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.9e-22
Identity = 52/104 (50.00%), Postives = 72/104 (69.23%), Query Frame = 0


Query: 73  ISKEEVKFVMEKLEFFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQTFNVFDKNRDGC 132
           IS+EE + VM  L  F +D+++ E +   +E+ ++F E E SLEE+KQ F+VFD+N+DG 
Sbjct: 83  ISREEAEMVMRSLGLFYNDDQLQEQY-SAKEVSSLFEEKEASLEEVKQAFDVFDENKDGF 142

Query: 133 IDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
           IDA ELQ +L +L   QG ++ +C  MI   D NKDGKIDFNEF
Sbjct: 143 IDAIELQRVLTILGFKQGSYLDNCLVMIRSLDGNKDGKIDFNEF 185

[Close]

BLAST of Cp4.1LG04g01570 vs. TAIR 10
Match: AT5G39670.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 84.7 bits (208), Expect = 1.0e-16
Identity = 46/108 (42.59%), Postives = 66/108 (61.11%), Query Frame = 0


Query: 70  NSSISKEEVKFVMEKLEFFCSDE-EIDESWLGGEEIGAIFYESEPSLEELKQTFNVFDKN 129
           +  + +E+V  VM+ L      E E  +     +E+  +F E EPSLEE+KQ F+VFD+N
Sbjct: 88  DDGLCREDVGMVMKSLGLSTDQENEGLQKQYSSKEVSNLFEEKEPSLEEVKQAFDVFDEN 147

Query: 130 RDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
           RDG ID  +LQ +L +L   QG  + +C RMI  +D +KDG+IDF  F
Sbjct: 148 RDGFIDPIDLQRVLTILGLKQGSNLENCRRMIRSFDGSKDGRIDFYGF 195

[Close]

BLAST of Cp4.1LG04g01570 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 72.0 bits (175), Expect = 7.0e-13
Identity = 42/120 (35.00%), Postives = 68/120 (56.67%), Query Frame = 0


Query: 70  NSSISKEEVKFVMEKLEFFCSD-------EEID---ESWLGGEEIGAIF---YESEPSLE 129
           +  I+K+E+   +E L  +  D       E+ID   + ++  EE G ++    E     E
Sbjct: 18  DGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEFGGLYQTIMEERDEEE 77

Query: 130 ELKQTFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
           ++++ FNVFD+NRDG I  +EL+S+L  L   QG  + DC RMI++ D + DG ++F EF
Sbjct: 78  DMREAFNVFDQNRDGFITVEELRSVLASLGLKQGRTLEDCKRMISKVDVDGDGMVNFKEF 137

[Close]

BLAST of Cp4.1LG04g01570 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 65.5 bits (158), Expect = 6.6e-11
Identity = 42/117 (35.90%), Postives = 65/117 (55.56%), Query Frame = 0


Query: 63  SEATQHKNSSISKEEVKFVMEKLEF---FCSDEEIDESWLGGEEIGAIFYESEPSLEELK 122
           SE  +     I  +E+  ++EK++     C D  IDE    GE    I  E +   E++K
Sbjct: 27  SETLRSLGIYIPDKELTQMIEKIDVNGDGCVD--IDEF---GELYKTIMDEEDEEEEDMK 86

Query: 123 QTFNVFDKNRDGCIDADELQSMLGLLVPNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
           + FNVFD+N DG I  DEL+++L  L   QG  + DC +MI + D + DG++++ EF
Sbjct: 87  EAFNVFDQNGDGFITVDELKAVLSSLGLKQGKTLDDCKKMIKKVDVDGDGRVNYKEF 138

[Close]

BLAST of Cp4.1LG04g01570 vs. TAIR 10
Match: AT3G47480.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 63.9 bits (154), Expect = 1.9e-10
Identity = 49/150 (32.67%), Postives = 78/150 (52.00%), Query Frame = 0


Query: 30  SFVDRIHDLISQPWSLFHSPPKEKASPQGNSLESEATQHK-NSSISKEEVKFVMEKLE-- 89
           SF  R H   S  + LF S    K + +   ++     ++ +   S EEVK +++  E  
Sbjct: 34  SFPFRFHLFFSNAYILFTSIRNNKQNTELPIIKKVVVPNRADIKTSVEEVKAIIDDSEAL 93

Query: 90  FFCSDEEIDESWLGGEEIGAIFYESEPSLEELKQTFNVFDKNRDGCIDADELQSMLGLLV 149
           + C  EE +E  L   E+           E +K+ F +FD+N+DG ID +EL+ +L LL 
Sbjct: 94  YECLIEEGEEYLLEKNEM--------MGKEIVKEAFRLFDENQDGFIDENELKHVLSLLG 153

Query: 150 PNQGVFIHDCHRMIARYDHNKDGKIDFNEF 177
            ++   + +C +M+  YD N+DGKIDF EF
Sbjct: 154 YDECTKM-ECRKMVKVYDENRDGKIDFYEF 174

The following BLAST results are available for this feature:

BLAST of Cp4.1LG04g01570 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9MBG5	6.8e-21	50.00	Probable calcium-binding protein CML45 OS=Arabidopsis thaliana OX=3702 GN=CML45 ...	[more]
Q93Z27	1.5e-15	42.59	Probable calcium-binding protein CML46 OS=Arabidopsis thaliana OX=3702 GN=CML46 ...	[more]
Q9SRR7	9.9e-12	35.00	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9LNE7	9.3e-10	35.90	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SN89	2.7e-09	32.67	Probable calcium-binding protein CML47 OS=Arabidopsis thaliana OX=3702 GN=CML47 ...	[more]

BLAST of Cp4.1LG04g01570 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023530007.1	2.19e-121	100.00	probable calcium-binding protein CML30 [Cucurbita pepo subsp. pepo]	[more]
XP_023003075.1	8.53e-119	97.16	probable calcium-binding protein CML30 [Cucurbita maxima]	[more]
XP_022927644.1	4.93e-118	97.73	probable calcium-binding protein CML30 [Cucurbita moschata]	[more]
KAG6588366.1	2.00e-117	97.16	putative calcium-binding protein CML45, partial [Cucurbita argyrosperma subsp. s...	[more]
KAG7022215.1	1.16e-116	96.59	putative calcium-binding protein CML30, partial [Cucurbita argyrosperma subsp. a...	[more]

BLAST of Cp4.1LG04g01570 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KQS0	4.13e-119	97.16	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114967...	[more]
A0A6J1EIK8	2.38e-118	97.73	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A6J1J5Q6	1.13e-52	54.55	probable calcium-binding protein CML30 OS=Cucurbita maxima OX=3661 GN=LOC1114819...	[more]
A0A6J1F854	5.01e-51	52.43	probable calcium-binding protein CML30 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A0A0LHQ5	2.02e-42	54.34	Calcium-binding EF hand family protein OS=Cucumis sativus OX=3659 GN=Csa_3G82501...	[more]

BLAST of Cp4.1LG04g01570 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G29000.1	4.9e-22	50.00	Calcium-binding EF-hand family protein	[more]
AT5G39670.1	1.0e-16	42.59	Calcium-binding EF-hand family protein	[more]
AT3G07490.1	7.0e-13	35.00	ARF-GAP domain 11	[more]
AT1G05990.1	6.6e-11	35.90	EF hand calcium-binding protein family	[more]
AT3G47480.1	1.9e-10	32.67	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 117..145 e-value: 7.4E-6 score: 35.5 coord: 155..183 e-value: 2.9 score: 14.0
IPR002048	EF-hand domain	PFAM	PF00036	EF-hand_1	coord: 117..142 e-value: 2.1E-7 score: 30.0
IPR002048	EF-hand domain	PFAM	PF13202	EF-hand_5	coord: 162..176 e-value: 0.0031 score: 16.9
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 151..186 score: 9.715397
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 113..148 score: 15.322372
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 117..176 e-value: 1.51086E-15 score: 66.8025
None	No IPR available	GENE3D	1.10.238.10		coord: 97..179 e-value: 1.6E-17 score: 65.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..68
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 49..70
None	No IPR available	PANTHER	PTHR10891:SF745	CALCIUM-BINDING PROTEIN CML46-RELATED	coord: 23..176
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 23..176
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 126..138
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 164..176
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 68..176

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG04g01570.1	Cp4.1LG04g01570.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0005509	calcium ion binding