Cp4.1LG03g04950 (gene) Cucurbita pepo (MU‐CU‐16) v4.1

Overview


Name	Cp4.1LG03g04950
Type	gene
Organism	Cucurbita pepo (Cucurbita pepo (MU‐CU‐16) v4.1)
Description	Pectin lyase-like superfamily protein
Location	Cp4.1LG03: 5102548 .. 5104795 (-)
RNA-Seq Expression	Cp4.1LG03g04950
Synteny	Cp4.1LG03g04950

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTCCAAAATGAACTCTTCTTTTTGTTCCTTTGCTTTGTGTATTACACTCATAATCTTCTTCTTTCCTGTTCATGGACGATGGCATAACCATGCAAAGAACCACAAACATTCTCACCATCATCACCATTTCACCATTTCACAACCTCCTTCTCCTTCTCCTTCCTCTTCCCCCGAACCCGCGAATCCTCCCGACGACAACGACGGAGGGTCATCTTTCAACGCCACCGGCTTTTTCGACGTACGTCATTATGGCGCCATTGGAGATGGCACTACAGACGACACGGAGGCCTTCAAGATAGCATGGGACACAGCCTGTCAAAGCGACAACGACGACTATACAGTTATTCTTGTTCCATATGGCTTCTCGTTTATGATCCAATCGACAATTTTCACCGGGCCGTGCAAACATGGCGTCGTGTTTCAAGTTGATGGCACTCTCATAGCTCCAGACGGGCCCGACGCTTGGCCGAGGAGTTACAGTGCTCATCAATGGCTGGTTTTTTATCGAGTTAATAACATGTCGCTTCGAGGAAACGGTGTTATTGACGGGCGAGGACAAAAATGGTGGGATCTTCCCTGCAAACCCCACAGAGTAAGAGACATGACATTGATACTATGATGTTTTTTGAACATAACTATGAGCTTCTAATTATATTCCAATTTTCAGGGAGGAAAGGGAAGAACAAAGGCAGGCCCTTGTGATAGTCCAATTGTAAGTTAATTCAAGTCCAATTCTACAAAATGTTGTTGTTTATGAGCTTAGAATATGAACAATTGAAAGGGTTTTTGCAGGCCATAAGATTCTTCATGAGCACAAACTTGACCGTTGAAGGGCTTAGAATCAAAGACAGCCCACAATTCCACTTCAGGTTCGACAATTGTAGAGATGTTTATATTGATTCTCTTAACATAACAGCTCCTGCTTTGAGTCCAAACACCGATGGAATTCACATTGAGAATACAAATGGGGTTCAGATACTCAATTCAGTTGTTGCCAATGGTTAGTTGTGATCAAGAACATTGTTTTTTTATCATTTTCTTTGCTTTTTTTCCACGAAAATTGTAAATTTGGGTTGAATTTTTTCTTACCCAGGTGATGATTGTGTTTCAATTGGTTCGGGTTCTTATAATGTTGTTATACGAAATATTACGTGTGGACCAGGTCATGGAATTAGGTAACATCGAAAGCTTATTATAATTCCGTCCTCTATTCATTACTAGAAATAATGGATACGATAGCATACAAAAAATGTTATTGTAGACTTTTTATAGCGTTTTATTTGTATGTCGTGACAACCTATGTTATTAAAAGTCTGAGACTTTTTATGTGAAGACCTCACATTGGTTGGAGAAGAGAACGAAACATTTCTTATAAGGGCGTAGAAACCTTTCACCACGCGTTTTAAAAACCTTGAGGGAAAGCCCGGAAGGGAAAGCTCAAATAGGACAATATTTACTAGTGATGGGTTTGGTCGGTTACAAATGATATCAAAGCTAGACACCGAGTGGTGTGCTAGCGAGGATGCTGGCCCCTAAGGTGGGTGAGATTGTGGGTGTGTAAATCTCTCCCTAACATACGCGTTTTAAAACCTTTAGGAGAAGTTCACAAAAGAAAGTCTGAAGAGGACAATATCTGCTAGCGAGTTTGGGTTGTTACATTTTGTTTTTATAGTTATAATAGATGCTATGATAGCGTATAAAACTACTTGTTCATACGCTCAACGTCCATTGATCTTTTCCATCTCCAGCATTGGCAGCCTAGGCAATCACAACTCCCGAGCATGTGTGTCAAACATAACAGTTCGAGACTCAATAATCAAGGTCTCAGACAACGGTGTTCGGATCAAGACATGGCAAGGTGGGTTTGGATCCGTAAGAGGAGTAAGTTTCAATAACATTCATATGGACAACGTTAGGAACCCGATCATCATAGACCAATTCTATTGCCTCAACAAAGGGTGTCTCAATCAAACCTCAGCCGTGGTTGTATCAGACATAAGCTATATTGACATCAAAGGAAGTTATGATATTAGAAACCCACCAATGCACTTTGGGTGCAGTGATTCTATGCCTTGTGTGAACCTAACACTTGCAAATATCGAGCTTTTTCCTTCAGAAGGTGACATCTTTATGGACCCATTTTGTTGGAATGCGTATGGGGACTTGCAAACCCTAACAATCCCTCCACTTGGGTGCTTGCTTGAAGCCTCCTCGAGGTCTATCTTGGAGACTCAAAGGGATTATTGC

mRNA sequence

TTTCCAAAATGAACTCTTCTTTTTGTTCCTTTGCTTTGTGTATTACACTCATAATCTTCTTCTTTCCTGTTCATGGACGATGGCATAACCATGCAAAGAACCACAAACATTCTCACCATCATCACCATTTCACCATTTCACAACCTCCTTCTCCTTCTCCTTCCTCTTCCCCCGAACCCGCGAATCCTCCCGACGACAACGACGGAGGGTCATCTTTCAACGCCACCGGCTTTTTCGACGTACGTCATTATGGCGCCATTGGAGATGGCACTACAGACGACACGGAGGCCTTCAAGATAGCATGGGACACAGCCTGTCAAAGCGACAACGACGACTATACAGTTATTCTTGTTCCATATGGCTTCTCGTTTATGATCCAATCGACAATTTTCACCGGGCCGTGCAAACATGGCGTCGTGTTTCAAGTTGATGGCACTCTCATAGCTCCAGACGGGCCCGACGCTTGGCCGAGGAGTTACAGTGCTCATCAATGGCTGGTTTTTTATCGAGTTAATAACATGTCGCTTCGAGGAAACGGTGTTATTGACGGGCGAGGACAAAAATGGTGGGATCTTCCCTGCAAACCCCACAGAGGAGGAAAGGGAAGAACAAAGGCAGGCCCTTGTGATAGTCCAATTGCCATAAGATTCTTCATGAGCACAAACTTGACCGTTGAAGGGCTTAGAATCAAAGACAGCCCACAATTCCACTTCAGGTTCGACAATTGTAGAGATGTTTATATTGATTCTCTTAACATAACAGCTCCTGCTTTGAGTCCAAACACCGATGGAATTCACATTGAGAATACAAATGGGGTTCAGATACTCAATTCAGTTGTTGCCAATGAAGGTGACATCTTTATGGACCCATTTTGTTGGAATGCGTATGGGGACTTGCAAACCCTAACAATCCCTCCACTTGGGTGCTTGCTTGAAGCCTCCTCGAGGTCTATCTTGGAGACTCAAAGGGATTATTGC

Coding sequence (CDS)

ATGAACTCTTCTTTTTGTTCCTTTGCTTTGTGTATTACACTCATAATCTTCTTCTTTCCTGTTCATGGACGATGGCATAACCATGCAAAGAACCACAAACATTCTCACCATCATCACCATTTCACCATTTCACAACCTCCTTCTCCTTCTCCTTCCTCTTCCCCCGAACCCGCGAATCCTCCCGACGACAACGACGGAGGGTCATCTTTCAACGCCACCGGCTTTTTCGACGTACGTCATTATGGCGCCATTGGAGATGGCACTACAGACGACACGGAGGCCTTCAAGATAGCATGGGACACAGCCTGTCAAAGCGACAACGACGACTATACAGTTATTCTTGTTCCATATGGCTTCTCGTTTATGATCCAATCGACAATTTTCACCGGGCCGTGCAAACATGGCGTCGTGTTTCAAGTTGATGGCACTCTCATAGCTCCAGACGGGCCCGACGCTTGGCCGAGGAGTTACAGTGCTCATCAATGGCTGGTTTTTTATCGAGTTAATAACATGTCGCTTCGAGGAAACGGTGTTATTGACGGGCGAGGACAAAAATGGTGGGATCTTCCCTGCAAACCCCACAGAGGAGGAAAGGGAAGAACAAAGGCAGGCCCTTGTGATAGTCCAATTGCCATAAGATTCTTCATGAGCACAAACTTGACCGTTGAAGGGCTTAGAATCAAAGACAGCCCACAATTCCACTTCAGGTTCGACAATTGTAGAGATGTTTATATTGATTCTCTTAACATAACAGCTCCTGCTTTGAGTCCAAACACCGATGGAATTCACATTGAGAATACAAATGGGGTTCAGATACTCAATTCAGTTGTTGCCAATGAAGGTGACATCTTTATGGACCCATTTTGTTGGAATGCGTATGGGGACTTGCAAACCCTAACAATCCCTCCACTTGGGTGCTTGCTTGAAGCCTCCTCGAGGTCTATCTTGGAGACTCAAAGGGATTATTGC

Protein sequence

MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSPEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANEGDIFMDPFCWNAYGDLQTLTIPPLGCLLEASSRSILETQRDYC

Homology

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy Swiss-Prot
Match: Q949Z1 (Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 5.9e-71
Identity = 142/308 (46.10%), Postives = 187/308 (60.71%), Query Frame = 0


Query: 12  ITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSPEPANPPD--------- 71
           + + I    + G + N A+   H H   H  ++ P  P P    E ANPPD         
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH-GVTHPLPPPPPPPLETANPPDQVPSDPYPS 67

Query: 72  ------DNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVP 131
                 D+D G        FDV  +GA+GDG+ DDT AF+ AW  AC  ++    V+L P
Sbjct: 68  PDPAPGDSDSGC------VFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESG---VVLAP 127

Query: 132 YGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGN 191
            G  F I STIF+GPCK G+VFQ+DG L+ PDGP+ WP   + +QWLVFYR++  +  G 
Sbjct: 128 EGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGK 187

Query: 192 GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFR 251
           G ++G GQKWWDLPCKPHRG  G + +GPC SP  IRFFMS N+ V+GLRI++SPQFH +
Sbjct: 188 GTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMK 247

Query: 252 FDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANEGD-IFMDPFCWNAYGD 304
           FD C+ V I+ + I++P LSPNTDGIH+ NT  V I NSVV+N  D I +   C +   D
Sbjct: 248 FDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDV--D 303

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 169.1 bits (427), Expect = 8.3e-41
Identity = 82/206 (39.81%), Postives = 119/206 (57.77%), Query Frame = 0


Query: 78  VRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFSFMIQSTIFTGPCKHGVV 137
           V ++GA G+G TDDT+AF  AW TAC S     T ILVP  ++ +++    +GPCK  + 
Sbjct: 55  VGNFGAKGNGVTDDTKAFADAWKTACSSKVK--TRILVPENYTCLLRPIDLSGPCKARLT 114

Query: 138 FQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGG 197
            Q+ GT+IAP+ PD W    +  +WL F+ ++ +++ G G ++G GQ+WW+  CK     
Sbjct: 115 LQISGTIIAPNDPDVW-EGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCK----- 174

Query: 198 KGRTKAGPC-DSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNCRDVYIDSLNITAPALS 257
                + PC  +P A+ F    N+ VE L + DS Q H    +CR V I  L + APA S
Sbjct: 175 --HNHSNPCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATS 234

Query: 258 PNTDGIHIENTNGVQILNSVVANEGD 283
           PNTDGIHI  + G+ I N+ V+   D
Sbjct: 235 PNTDGIHISVSRGIVIDNTTVSTGDD 250

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy Swiss-Prot
Match: P43212 (Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.5e-39
Identity = 83/215 (38.60%), Postives = 117/215 (54.42%), Query Frame = 0


Query: 68  SSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFSFMIQSTI 127
           S  +A   F+V  YGA+GDG  D TEAF  AW  AC+  +    ++LVP    F++ +  
Sbjct: 51  SRHDAINIFNVEKYGAVGDGKHDCTEAFSTAWQAACKKPS---AMLLVPGNKKFVVNNLF 110

Query: 128 FTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVIDGRGQKWW 187
           F GPC+    F+VDG + A   P +W  +     WL F ++   +L G GVIDG+G++WW
Sbjct: 111 FNGPCQPHFTFKVDGIIAAYQNPASWKNN---RIWLQFAKLTGFTLMGKGVIDGQGKQWW 170

Query: 188 DLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNCRDVYIDS 247
              CK      GR      D P AI+F  ST L ++GL++ +SP+FH  F NC  V I  
Sbjct: 171 AGQCK---WVNGREICNDRDRPTAIKFDFSTGLIIQGLKLMNSPEFHLVFGNCEGVKIIG 230

Query: 248 LNITAPALSPNTDGIHIENTNGVQILNSVVANEGD 283
           ++ITAP  SPNTDGI I  +    +  + +    D
Sbjct: 231 ISITAPRDSPNTDGIDIFASKNFHLQKNTIGTGDD 256

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy Swiss-Prot
Match: Q02096 (Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1)

HSP 1 Score: 161.8 bits (408), Expect = 1.3e-38
Identity = 84/218 (38.53%), Postives = 121/218 (55.50%), Query Frame = 0


Query: 66  GGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFSFMIQS 125
           GG   +      V  +GA GDG TDDT+AF+ AW  AC S     +V++VP   +++++ 
Sbjct: 75  GGPETSPDTDISVDDFGARGDG-TDDTKAFEKAWKDACSSG----SVLIVPENKNYLLKQ 134

Query: 126 TIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVIDGRGQK 185
             F+GPCK  +  ++ GT+ A      W   ++  +W+ F  ++N++L G G  +G G+ 
Sbjct: 135 ITFSGPCKSDLRVKIRGTIEASSDQSDWV-GHNRKRWIEFEDISNLTLEGGGTSNGNGET 194

Query: 186 WWDLPCKPHRGGKGRTKAGPCDS-PIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNCRDVY 245
           WWD  CK       R K+ PC S P A+ F    NL V  L IKDS + H  FD C+DV 
Sbjct: 195 WWDSSCK-------RKKSLPCKSAPTALTFRSCKNLIVSDLSIKDSQKMHLSFDKCQDVI 254

Query: 246 IDSLNITAPALSPNTDGIHIENTNGVQILNSVVANEGD 283
             +L +TAP  SPNTDGIHI  T  + ++NSV+    D
Sbjct: 255 ASNLMVTAPEHSPNTDGIHITGTQRIHVMNSVIGTGDD 279

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 3.8e-38
Identity = 85/215 (39.53%), Postives = 116/215 (53.95%), Query Frame = 0


Query: 68  SSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFSFMIQSTI 127
           S  +A   F+V  YGA+GDG  D TEAF   W+ AC+  +    V+LVP    F + + +
Sbjct: 51  SRHDAATVFNVEQYGAVGDGKHDSTEAFATTWNAACKKAS---AVLLVPANKKFFVNNLV 110

Query: 128 FTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVIDGRGQKWW 187
           F GPC+  + F+VDGT++A   P  W  S     WL F ++ + +L G GVIDG+GQ+WW
Sbjct: 111 FRGPCQPHLSFKVDGTIVAQPDPARWKNS---KIWLQFAQLTDFNLMGTGVIDGQGQQWW 170

Query: 188 DLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNCRDVYIDS 247
              CK      GRT     + P AI+   S ++TV+ L + +SP+FH  F  C  V I  
Sbjct: 171 AGQCKV---VNGRTVCNDRNRPTAIKIDYSKSVTVKELTLMNSPEFHLVFGECEGVKIQG 230

Query: 248 LNITAPALSPNTDGIHIENTNGVQILNSVVANEGD 283
           L I AP  SPNTDGI I  +    I   V+    D
Sbjct: 231 LKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDD 256

[Close]

BLAST of Cp4.1LG03g04950 vs. NCBI nr
Match: XP_023529142.1 (polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 624 bits (1609), Expect = 1.13e-222
Identity = 323/473 (68.29%), Postives = 323/473 (68.29%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSPEPANP 60
           MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSPEPANP
Sbjct: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSPEPANP 60

Query: 61  PDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFS 120
           PDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFS
Sbjct: 61  PDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFS 120

Query: 121 FMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVID 180
           FMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVID
Sbjct: 121 FMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVID 180

Query: 181 GRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNC 240
           GRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNC
Sbjct: 181 GRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNC 240

Query: 241 RDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN--------------------- 300
           RDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN                     
Sbjct: 241 RDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANGDDCVSIGSGSYNVVIRNITC 300

Query: 301 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 GPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGSVRGVSFNNIHMD 360

[Close]

BLAST of Cp4.1LG03g04950 vs. NCBI nr
Match: XP_022935453.1 (polygalacturonase At1g48100-like [Cucurbita moschata])

HSP 1 Score: 595 bits (1533), Expect = 5.18e-211
Identity = 310/479 (64.72%), Postives = 316/479 (65.97%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSP----- 60
           MNS FCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHH HFTISQPPSPSPS SP     
Sbjct: 1   MNSPFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPSPPLSPS 60

Query: 61  -EPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVIL 120
            EPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFK+AWDTACQSDNDDY VIL
Sbjct: 61  PEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDNDDYAVIL 120

Query: 121 VPYGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLR 180
           VPYGFSFMIQSTIFTGPCKHG+VFQVDGT IAPDGPDAWP+SYSAHQWLVFYRVNNMSLR
Sbjct: 121 VPYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVFYRVNNMSLR 180

Query: 181 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFH 240
           GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMS+NLTVEGLRIKDSPQFH
Sbjct: 181 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH 240

Query: 241 FRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN--------------- 300
           FRFDNCRDVYIDSL ITAPA SPNTDGIHIENTNGVQILNSVVAN               
Sbjct: 241 FRFDNCRDVYIDSLTITAPAWSPNTDGIHIENTNGVQILNSVVANGDDCISIGSGSYNVD 300

Query: 301 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 IRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGAVRGVSF 360

[Close]

BLAST of Cp4.1LG03g04950 vs. NCBI nr
Match: XP_022983334.1 (polygalacturonase At1g48100-like [Cucurbita maxima])

HSP 1 Score: 574 bits (1480), Expect = 7.94e-203
Identity = 305/488 (62.50%), Postives = 315/488 (64.55%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPS---------- 60
           MNS  CSFALCI LIIFFFPVHGRWHNHA NHKHSHHHHHFTISQPPSPS          
Sbjct: 1   MNSPLCSFALCIILIIFFFPVHGRWHNHA-NHKHSHHHHHFTISQPPSPSTSASASTSTS 60

Query: 61  PSSSPEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSD---- 120
           PS SPEPANPPDDNDGGSSFN+TGFFDVRHYGAIGDGTTDDTEAFK+AWDTACQSD    
Sbjct: 61  PSPSPEPANPPDDNDGGSSFNSTGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDDKNN 120

Query: 121 -NDDYTVILVPYGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVF 180
            ND++ VILVPYGFSFMIQSTIF+GPCKHGVVFQVDGTLIAPDGPD WP+SYSAHQWLVF
Sbjct: 121 NNDNHAVILVPYGFSFMIQSTIFSGPCKHGVVFQVDGTLIAPDGPDTWPKSYSAHQWLVF 180

Query: 181 YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGL 240
           YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKG TKAGPCDSPIAIRFFMS+NL+VEGL
Sbjct: 181 YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGSTKAGPCDSPIAIRFFMSSNLSVEGL 240

Query: 241 RIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN------ 300
           RIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN      
Sbjct: 241 RIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANGDDCIS 300

Query: 301 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 IGSGSYNVDIQNITCGPGHGISIGSLGNHNSLACVSNITVRDSIIKVSDNGVRIKTWQGG 360

[Close]

BLAST of Cp4.1LG03g04950 vs. NCBI nr
Match: KAG6581318.1 (Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 548 bits (1411), Expect = 1.39e-193
Identity = 283/406 (69.70%), Postives = 300/406 (73.89%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSS----SPE 60
           MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHH HFTISQPPSPSPS     SPE
Sbjct: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPPLSPSPE 60

Query: 61  PANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVP 120
           PANPPD+NDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFK+AWDTACQSDNDDY VILVP
Sbjct: 61  PANPPDNNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDNDDYAVILVP 120

Query: 121 YGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGN 180
           YGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWP+SYSAHQWLVFYRVNNMSLRGN
Sbjct: 121 YGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPKSYSAHQWLVFYRVNNMSLRGN 180

Query: 181 GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHF- 240
           GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMS+NLTVEGLRIKDSPQFHF 
Sbjct: 181 GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFS 240

Query: 241 --------------------------------------------------RFDNCRD-VY 300
                                                               DN R+ + 
Sbjct: 241 IGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRNPII 300

Query: 301 IDS--------LNITAPALSPNTDGIHIENTNGVQ-------------ILNSVVAN---- 323
           ID         LN T+  +  +   I I+ +  ++              +N  +AN    
Sbjct: 301 IDQFYCLNKGCLNQTSAVVVSDISYIDIKGSYDIRSPPMHFGCSDSMPCVNLTLANIELF 360

[Close]

BLAST of Cp4.1LG03g04950 vs. NCBI nr
Match: XP_038905655.1 (polygalacturonase At1g48100-like [Benincasa hispida])

HSP 1 Score: 479 bits (1232), Expect = 2.73e-165
Identity = 257/476 (53.99%), Postives = 286/476 (60.08%), Query Frame = 0


Query: 5   FCSFALCITLIIFFF----PVHGRWHNHAKNHK-HSHHHHHFTISQPPSPSPSSSPEPAN 64
           F S A CIT IIFFF    PVHGRWHNHAK HK HSHHH+HFTISQPPSP P+S P+   
Sbjct: 6   FSSLAFCITFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHNHFTISQPPSPPPTSPPDD-- 65

Query: 65  PPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDD--YTVILVPY 124
             DDN G SS N+TG FDVR++GAIGDGT DDT AFK+AWDTACQ D+DD  Y VIL+PY
Sbjct: 66  --DDNGGSSSINSTGIFDVRNFGAIGDGTADDTMAFKMAWDTACQRDDDDDNYAVILIPY 125

Query: 125 GFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNG 184
           GFSFMIQSTIFTGPCK+GV FQV GTL+APDGPDAWP+SYSAHQWLVFYRV NMSLRGNG
Sbjct: 126 GFSFMIQSTIFTGPCKNGVEFQVGGTLMAPDGPDAWPKSYSAHQWLVFYRVYNMSLRGNG 185

Query: 185 VIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRF 244
           +IDGRGQKWWDLPCKPH+G KGRT + PCDSPIAIRFFMS+NLTVEGLRIKDSPQFHFRF
Sbjct: 186 LIDGRGQKWWDLPCKPHKGVKGRTMSEPCDSPIAIRFFMSSNLTVEGLRIKDSPQFHFRF 245

Query: 245 DNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN------------------ 304
           DNC+DV ID ++ITAPALSPNTDGIHIENTNGVQI NS VAN                  
Sbjct: 246 DNCKDVIIDGIHITAPALSPNTDGIHIENTNGVQIFNSEVANGDDCISIGSGSYNVDIRN 305

Query: 305 ------------------------------------------------------------ 323
                                                                       
Sbjct: 306 ITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYGAVRGVSFNNI 365

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy TrEMBL
Match: A0A6J1FAK7 (polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 PE=3 SV=1)

HSP 1 Score: 595 bits (1533), Expect = 2.51e-211
Identity = 310/479 (64.72%), Postives = 316/479 (65.97%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSP----- 60
           MNS FCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHH HFTISQPPSPSPS SP     
Sbjct: 1   MNSPFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHPHFTISQPPSPSPSPSPPLSPS 60

Query: 61  -EPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVIL 120
            EPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFK+AWDTACQSDNDDY VIL
Sbjct: 61  PEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDNDDYAVIL 120

Query: 121 VPYGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLR 180
           VPYGFSFMIQSTIFTGPCKHG+VFQVDGT IAPDGPDAWP+SYSAHQWLVFYRVNNMSLR
Sbjct: 121 VPYGFSFMIQSTIFTGPCKHGIVFQVDGTFIAPDGPDAWPKSYSAHQWLVFYRVNNMSLR 180

Query: 181 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFH 240
           GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMS+NLTVEGLRIKDSPQFH
Sbjct: 181 GNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSSNLTVEGLRIKDSPQFH 240

Query: 241 FRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN--------------- 300
           FRFDNCRDVYIDSL ITAPA SPNTDGIHIENTNGVQILNSVVAN               
Sbjct: 241 FRFDNCRDVYIDSLTITAPAWSPNTDGIHIENTNGVQILNSVVANGDDCISIGSGSYNVD 300

Query: 301 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 IRNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGAVRGVSF 360

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy TrEMBL
Match: A0A6J1J721 (polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481948 PE=3 SV=1)

HSP 1 Score: 574 bits (1480), Expect = 3.84e-203
Identity = 305/488 (62.50%), Postives = 315/488 (64.55%), Query Frame = 0


Query: 1   MNSSFCSFALCITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPS---------- 60
           MNS  CSFALCI LIIFFFPVHGRWHNHA NHKHSHHHHHFTISQPPSPS          
Sbjct: 1   MNSPLCSFALCIILIIFFFPVHGRWHNHA-NHKHSHHHHHFTISQPPSPSTSASASTSTS 60

Query: 61  PSSSPEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSD---- 120
           PS SPEPANPPDDNDGGSSFN+TGFFDVRHYGAIGDGTTDDTEAFK+AWDTACQSD    
Sbjct: 61  PSPSPEPANPPDDNDGGSSFNSTGFFDVRHYGAIGDGTTDDTEAFKMAWDTACQSDDKNN 120

Query: 121 -NDDYTVILVPYGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVF 180
            ND++ VILVPYGFSFMIQSTIF+GPCKHGVVFQVDGTLIAPDGPD WP+SYSAHQWLVF
Sbjct: 121 NNDNHAVILVPYGFSFMIQSTIFSGPCKHGVVFQVDGTLIAPDGPDTWPKSYSAHQWLVF 180

Query: 181 YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGL 240
           YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKG TKAGPCDSPIAIRFFMS+NL+VEGL
Sbjct: 181 YRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGSTKAGPCDSPIAIRFFMSSNLSVEGL 240

Query: 241 RIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN------ 300
           RIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN      
Sbjct: 241 RIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANGDDCIS 300

Query: 301 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 IGSGSYNVDIQNITCGPGHGISIGSLGNHNSLACVSNITVRDSIIKVSDNGVRIKTWQGG 360

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy TrEMBL
Match: A0A0A0LEQ6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1)

HSP 1 Score: 471 bits (1211), Expect = 1.55e-162
Identity = 256/473 (54.12%), Postives = 281/473 (59.41%), Query Frame = 0


Query: 12  ITLIIFFF----PVHGRWHNHAKNHK-HSHHHHHFTISQPPSPSPSSSPEPANPPDDNDG 71
           +T IIFFF    PVHGRWHNHAK HK HSHHH HFTISQPPSPSPSS P   +PP+DN G
Sbjct: 1   MTFIIFFFCLLFPVHGRWHNHAKKHKQHSHHHDHFTISQPPSPSPSSEPA-GSPPEDNGG 60

Query: 72  --GSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQ----SDNDDYTVILVPYGFS 131
              SS N+T  FDVRH+GAIGDGT DDT AFK+AWDTACQ     +NDDY VILVPYGFS
Sbjct: 61  PSSSSINSTSIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDNNDDYGVILVPYGFS 120

Query: 132 FMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVID 191
           FMIQSTIFTGPCK+GVVFQVDGTL+APDGPDAWP+ YSAHQWLVFYRV NMSL+GNG+ID
Sbjct: 121 FMIQSTIFTGPCKNGVVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYRVYNMSLQGNGLID 180

Query: 192 GRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNC 251
           GRGQKWWDLPCKPH   KG+T  GPCDSPIAIRFFMS+ L VEG+RIKDSPQFHFRFDNC
Sbjct: 181 GRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSELRVEGVRIKDSPQFHFRFDNC 240

Query: 252 RDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN--------------------- 311
           +DVYIDSL+IT+PALSPNTDGIHIENTNGVQI NS+VAN                     
Sbjct: 241 KDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCVSIGSGSFNVEIRNITC 300

Query: 312 ------------------------------------------------------------ 323
                                                                       
Sbjct: 301 GPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGFGTVRGVSFNNINMD 360

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy TrEMBL
Match: A0A5D3DA93 (Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00750 PE=3 SV=1)

HSP 1 Score: 469 bits (1207), Expect = 1.37e-161
Identity = 256/486 (52.67%), Postives = 287/486 (59.05%), Query Frame = 0


Query: 7   SFALCITLIIFFF----PVHGRWHNHAKNHK-HSHHHHHFTISQPPSPSPSSSP------ 66
           SF  CIT IIFFF    PV+GRWHNHAK HK HSHHH+HFTISQPPSP PS SP      
Sbjct: 10  SFTFCITFIIFFFCLLFPVNGRWHNHAKKHKQHSHHHYHFTISQPPSPFPSPSPSPSPSP 69

Query: 67  ----EPANPPDDNDG-GSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDD- 126
               EP +PP+D+ G  SS N+T  FDVRH+GAIGDGT DDT AFK+AWDTACQ ++DD 
Sbjct: 70  SPSSEPQSPPEDDGGPASSINSTIIFDVRHFGAIGDGTADDTAAFKMAWDTACQRNDDDD 129

Query: 127 --YTVILVPYGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYR 186
             Y VILVPYGFSFMIQSTIFTGPC++G+VFQVDGTL+APDGPDAWP+ YSAHQWLVFYR
Sbjct: 130 NNYGVILVPYGFSFMIQSTIFTGPCRNGIVFQVDGTLMAPDGPDAWPKGYSAHQWLVFYR 189

Query: 187 VNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRI 246
           V NMSL+GNG+IDGRGQKWWDLPCKPH   KG+T  GPCDSPIAIRFFMS+ L VEG+RI
Sbjct: 190 VYNMSLQGNGLIDGRGQKWWDLPCKPH---KGKTMTGPCDSPIAIRFFMSSKLRVEGVRI 249

Query: 247 KDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN-------- 306
           KDSPQFHFRFDNC+DVYIDSL+IT+PALSPNTDGIHIENTNGVQI NS+VAN        
Sbjct: 250 KDSPQFHFRFDNCKDVYIDSLHITSPALSPNTDGIHIENTNGVQIFNSIVANGDDCISIG 309

Query: 307 ------------------------------------------------------------ 323
                                                                       
Sbjct: 310 SGSYNVEITNITCGPGHGISIGSLGNHNSRACVSNITVRDSIIKVSDNGVRIKTWQGGYG 369

[Close]

BLAST of Cp4.1LG03g04950 vs. ExPASy TrEMBL
Match: A0A6J1DNT2 (polygalacturonase At1g48100-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111022378 PE=3 SV=1)

HSP 1 Score: 454 bits (1169), Expect = 4.06e-156
Identity = 245/469 (52.24%), Postives = 278/469 (59.28%), Query Frame = 0


Query: 7   SFALCITLIIFF---FPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSPEPANPPDD 66
           SF  CI + IFF   F VHGR   HAK HKHSHHH H++ISQPPSP PS S   A+PPDD
Sbjct: 7   SFCFCIRIFIFFCLLFSVHGR---HAKKHKHSHHHDHYSISQPPSPPPSPSSGLASPPDD 66

Query: 67  NDGGSSFNATG-FFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYT-VILVPYGFSF 126
               SS N++G  FDVRH+GAIGDG  DDTEAFK+AWDTACQSD+DD + VILVPYGFSF
Sbjct: 67  GGYSSSLNSSGGIFDVRHFGAIGDGMVDDTEAFKMAWDTACQSDDDDSSAVILVPYGFSF 126

Query: 127 MIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVIDG 186
           +IQSTIFTGPCK GVVF +DGTL+APDGPDAWPRSYSAHQWLVFYRV NMSL+G+G+IDG
Sbjct: 127 LIQSTIFTGPCKKGVVFHIDGTLVAPDGPDAWPRSYSAHQWLVFYRVYNMSLQGDGLIDG 186

Query: 187 RGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNCR 246
           RGQKWWDLPCKPH+GG G+T  G CDSPIAIRFFMS+NLTVEGLRI+DSPQFHFRFDNCR
Sbjct: 187 RGQKWWDLPCKPHKGGTGKTTTGRCDSPIAIRFFMSSNLTVEGLRIRDSPQFHFRFDNCR 246

Query: 247 DVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVAN---------------------- 306
           DVYIDSL ITAPA SPNTDGIHIENTNGV+I NS+VAN                      
Sbjct: 247 DVYIDSLQITAPAFSPNTDGIHIENTNGVKIYNSIVANGDDCISIGSGSYNVDIKNITCG 306

Query: 307 ------------------------------------------------------------ 323
                                                                       
Sbjct: 307 PGHGISLGNHNSRACVSNVTVRDSTIKVSDNGVRIKTWQGGFGAVRGVSFNNIHMDNVRN 366

[Close]

BLAST of Cp4.1LG03g04950 vs. TAIR 10
Match: AT1G02460.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 352.8 bits (904), Expect = 2.9e-97
Identity = 174/306 (56.86%), Postives = 224/306 (73.20%), Query Frame = 0


Query: 12  ITLIIFFFPV-HGRWHNHAKNHKHSHHHHH--------FTISQPPS--PSPSSSPEPANP 71
           +TLI+    +   R H+H + HKH++H+HH         +ISQPP+  P P  SP P+ P
Sbjct: 15  MTLILTLMSLSEARNHHHKEKHKHNNHNHHSSKPKPPSSSISQPPTPPPGPPDSPAPSLP 74

Query: 72  PDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFS 131
           P  +D  +  N  G ++VR YGA+GDG TDDTEAFK AWD++C ++N+  +V+LVPYG++
Sbjct: 75  PSPSDDPADDN-NGIYNVRKYGAVGDGETDDTEAFKTAWDSSCNNENNTDSVLLVPYGYT 134

Query: 132 FMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVID 191
           FMIQSTIFTGPC+    FQVDGT++ PDGP++WP + S  QWLVFYRVN M+L+G GVID
Sbjct: 135 FMIQSTIFTGPCRSYQFFQVDGTIVTPDGPESWPSNISKRQWLVFYRVNGMALKGEGVID 194

Query: 192 GRGQKWWDLPCKPHRG-GKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDN 251
           GRGQKWWDLPCKPHR   K     GPCDSPIA+RFFMS+NL VEGL+IK+SPQFHFRFD 
Sbjct: 195 GRGQKWWDLPCKPHRSVNKSAIVTGPCDSPIALRFFMSSNLRVEGLQIKNSPQFHFRFDG 254

Query: 252 CRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANEGDIFMDPFCWNAYGDLQTL 306
           C+ V+++SL+ITAP LSPNTDGIHIEN+N V I NS+++N GD  +     +   D++ L
Sbjct: 255 CQGVHVESLHITAPPLSPNTDGIHIENSNSVTIYNSIISN-GDDCVSIGSGSYDVDIRNL 314

[Close]

BLAST of Cp4.1LG03g04950 vs. TAIR 10
Match: AT4G01890.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 293.9 bits (751), Expect = 1.6e-79
Identity = 142/269 (52.79%), Postives = 188/269 (69.89%), Query Frame = 0


Query: 40  HFTISQPPSPSPSSSPEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAW 99
           H   SQPPSPSP+    P +P          +    +DVR YGA+G+G  DDT +FK AW
Sbjct: 33  HHQASQPPSPSPN----PNDPSKSPSRSQDLDHEVVYDVRKYGAVGNGVADDTVSFKTAW 92

Query: 100 DTACQSD-NDDYTVILVPYGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYS 159
           D+AC ++ N+  +V+ VPYGF+FMI+STIFTGPC+    FQVDGT++  DGP +WP S +
Sbjct: 93  DSACSNNKNNTASVLHVPYGFTFMIRSTIFTGPCRSYQYFQVDGTIVPRDGPKSWPSSLN 152

Query: 160 AHQWLVFYRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTK-AGPCDSPIAIRFFMS 219
             QWL FYR+N M+L+G GVIDGRGQ WWDLPCKPH+    +TK AGPC+SP A+RFFMS
Sbjct: 153 KRQWLAFYRINGMALQGAGVIDGRGQNWWDLPCKPHQQNVNKTKLAGPCESPAALRFFMS 212

Query: 220 TNLTVEGLRIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVV 279
           +N+ V+GL IK+SPQ H + D C  V+I+SL I +P  SPNTDGIHIEN+N V+I NSV+
Sbjct: 213 SNVIVKGLSIKNSPQVHLKLDGCHVVHINSLRIISPPASPNTDGIHIENSNSVEIYNSVI 272

Query: 280 ANEGD-IFMDPFCWNAYGDLQTLTIPPLG 306
           +N  D + + P  ++   D++ +T  P G
Sbjct: 273 SNGDDCVSIGPGAYDI--DIRNITCGPGG 295

[Close]

BLAST of Cp4.1LG03g04950 vs. TAIR 10
Match: AT1G56710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 287.3 bits (734), Expect = 1.5e-77
Identity = 136/262 (51.91%), Postives = 183/262 (69.85%), Query Frame = 0


Query: 43  ISQPPSPSPSSSPEPANPPDDNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTA 102
           I+Q    SPS +P PA   +DN       A   FDV  +GAIGD +TDDT AFK+AWD A
Sbjct: 21  IAQSIYLSPSPAPNPAYNDNDN------IAPTVFDVTSFGAIGDCSTDDTSAFKMAWDAA 80

Query: 103 CQSDNDDYTVILVPYGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQW 162
           C S      ++LVPY F F+++ T F GPC+  +V Q+DG +++PDGP +WP +Y   QW
Sbjct: 81  CMSTGPKSALLLVPYTFCFLVKPTTFNGPCRTNLVLQIDGFIVSPDGPRSWPSNYQ-RQW 140

Query: 163 LVFYRVNNMSLRGNGVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTV 222
           ++FYRVN +S++G+GVI+GRGQKWW+LPCKPH+G  G T+ GPCDSP+AIR F S+ + +
Sbjct: 141 MMFYRVNGLSIQGSGVINGRGQKWWNLPCKPHKGLNGTTQTGPCDSPVAIRLFQSSKVRI 200

Query: 223 EGLRIKDSPQFHFRFDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANEGD 282
           +G+   +S QFH RFDNC DV +DS+ I APA SPNTDGIHIENT+ VQI NS+++N  D
Sbjct: 201 QGINFMNSAQFHVRFDNCSDVVVDSVIIKAPASSPNTDGIHIENTHNVQIRNSMISNGDD 260

Query: 283 -IFMDPFCWNAYGDLQTLTIPP 304
            I +   C+N   D++ +T  P
Sbjct: 261 CISIGAGCFNV--DIKNVTCGP 273

[Close]

BLAST of Cp4.1LG03g04950 vs. TAIR 10
Match: AT1G48100.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 269.2 bits (687), Expect = 4.2e-72
Identity = 142/308 (46.10%), Postives = 187/308 (60.71%), Query Frame = 0


Query: 12  ITLIIFFFPVHGRWHNHAKNHKHSHHHHHFTISQPPSPSPSSSPEPANPPD--------- 71
           + + I    + G + N A+   H H   H  ++ P  P P    E ANPPD         
Sbjct: 8   LVIYIISTVLFGHFTNVARARYHYHKGRH-GVTHPLPPPPPPPLETANPPDQVPSDPYPS 67

Query: 72  ------DNDGGSSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVP 131
                 D+D G        FDV  +GA+GDG+ DDT AF+ AW  AC  ++    V+L P
Sbjct: 68  PDPAPGDSDSGC------VFDVTSFGAVGDGSCDDTAAFQDAWKAACAVESG---VVLAP 127

Query: 132 YGFSFMIQSTIFTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGN 191
            G  F I STIF+GPCK G+VFQ+DG L+ PDGP+ WP   + +QWLVFYR++  +  G 
Sbjct: 128 EGGVFKITSTIFSGPCKPGLVFQLDGVLMPPDGPEEWPEKDNKNQWLVFYRLDGFTFSGK 187

Query: 192 GVIDGRGQKWWDLPCKPHRGGKGRTKAGPCDSPIAIRFFMSTNLTVEGLRIKDSPQFHFR 251
           G ++G GQKWWDLPCKPHRG  G + +GPC SP  IRFFMS N+ V+GLRI++SPQFH +
Sbjct: 188 GTVEGNGQKWWDLPCKPHRGPDGSSSSGPCASPTMIRFFMSNNIEVKGLRIQNSPQFHMK 247

Query: 252 FDNCRDVYIDSLNITAPALSPNTDGIHIENTNGVQILNSVVANEGD-IFMDPFCWNAYGD 304
           FD C+ V I+ + I++P LSPNTDGIH+ NT  V I NSVV+N  D I +   C +   D
Sbjct: 248 FDGCQGVLINEIQISSPKLSPNTDGIHLGNTRSVGIYNSVVSNGDDCISIGTGCSDV--D 303

[Close]

BLAST of Cp4.1LG03g04950 vs. TAIR 10
Match: AT1G70500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 170.2 bits (430), Expect = 2.6e-42
Identity = 83/216 (38.43%), Postives = 123/216 (56.94%), Query Frame = 0


Query: 68  SSFNATGFFDVRHYGAIGDGTTDDTEAFKIAWDTACQSDNDDYTVILVPYGFSFMIQSTI 127
           S ++     +V  + A GDG +DDT+AF  AW  AC + N   +V+LVP G S+++ +T 
Sbjct: 59  SQYSGKNLVNVDSFNASGDGVSDDTQAFIRAWTMACSAPN---SVLLVPQGRSYLVNATK 118

Query: 128 FTGPCKHGVVFQVDGTLIAPDGPDAWPRSYSAHQWLVFYRVNNMSLRGNGVIDGRGQKWW 187
           F GPC+  ++ Q+DGT+IAPD P  W   +    WL F ++  +  +GNGVIDG G KWW
Sbjct: 119 FDGPCQEKLIIQIDGTIIAPDEPSQWDPKF-PRNWLQFSKLQGVVFQGNGVIDGSGTKWW 178

Query: 188 DLPCKPHRGGKGRTKAGPC-DSPIAIRFFMSTNLTVEGLRIKDSPQFHFRFDNCRDVYID 247
              CK       + K+ PC  +P A+  + S+N+ V GL I++S Q H        V I 
Sbjct: 179 AASCK-------KNKSNPCVGAPTALTIYSSSNVYVRGLTIRNSQQMHLIIQRSTTVRIS 238

Query: 248 SLNITAPALSPNTDGIHIENTNGVQILNSVVANEGD 283
            + +T+P  SPNTDGIHI  +  V + +S ++   D
Sbjct: 239 RVMVTSPGDSPNTDGIHITASTDVVVQDSKISTGDD 263

The following BLAST results are available for this feature:

BLAST of Cp4.1LG03g04950 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q949Z1	5.9e-71	46.10	Polygalacturonase At1g48100 OS=Arabidopsis thaliana OX=3702 GN=At1g48100 PE=2 SV...	[more]
Q94AJ5	8.3e-41	39.81	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]
P43212	3.5e-39	38.60	Polygalacturonase OS=Cryptomeria japonica OX=3369 PE=1 SV=1	[more]
Q02096	1.3e-38	38.53	Polygalacturonase OS=Persea americana OX=3435 PE=2 SV=1	[more]
Q7M1E7	3.8e-38	39.53	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]

BLAST of Cp4.1LG03g04950 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023529142.1	1.13e-222	68.29	polygalacturonase At1g48100-like [Cucurbita pepo subsp. pepo]	[more]
XP_022935453.1	5.18e-211	64.72	polygalacturonase At1g48100-like [Cucurbita moschata]	[more]
XP_022983334.1	7.94e-203	62.50	polygalacturonase At1g48100-like [Cucurbita maxima]	[more]
KAG6581318.1	1.39e-193	69.70	Polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038905655.1	2.73e-165	53.99	polygalacturonase At1g48100-like [Benincasa hispida]	[more]

BLAST of Cp4.1LG03g04950 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FAK7	2.51e-211	64.72	polygalacturonase At1g48100-like OS=Cucurbita moschata OX=3662 GN=LOC111442323 P...	[more]
A0A6J1J721	3.84e-203	62.50	polygalacturonase At1g48100-like OS=Cucurbita maxima OX=3661 GN=LOC111481948 PE=...	[more]
A0A0A0LEQ6	1.55e-162	54.12	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G664600 PE=3 SV=1	[more]
A0A5D3DA93	1.37e-161	52.67	Polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold237G00...	[more]
A0A6J1DNT2	4.06e-156	52.24	polygalacturonase At1g48100-like isoform X2 OS=Momordica charantia OX=3673 GN=LO...	[more]

BLAST of Cp4.1LG03g04950 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G02460.1	2.9e-97	56.86	Pectin lyase-like superfamily protein	[more]
AT4G01890.1	1.6e-79	52.79	Pectin lyase-like superfamily protein	[more]
AT1G56710.1	1.5e-77	51.91	Pectin lyase-like superfamily protein	[more]
AT1G48100.1	4.2e-72	46.10	Pectin lyase-like superfamily protein	[more]
AT1G70500.1	2.6e-42	38.43	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita pepo (Zucchini) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 113..282 e-value: 6.9E-32 score: 110.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 67..295 e-value: 6.0E-55 score: 188.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 36..66
None	No IPR available	PANTHER	PTHR31375:SF119	POLYGALACTURONASE/GLYCOSIDE HYDROLASE FAMILY PROTEIN	coord: 32..282
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 32..282
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 72..305

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Cp4.1LG03g04950.1	Cp4.1LG03g04950.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity