CmoCh13G002760 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh13G002760
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
Descriptionprotein argonaute 5-like
LocationCmo_Chr13: 2260082 .. 2262225 (-)
RNA-Seq ExpressionCmoCh13G002760
SyntenyCmoCh13G002760
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCTAGTGGGCGCGGTGGTCGTGGTCGTGGTGCCGGAGGTCGTGGAGGGCAGTCATCTCCGGCAATTTCTCGCGGTGGTGGTGGTAGAAGTGGCGGAGGTAGAGGTGGTCAAGGCGGTGGTGGAAGAGGTGGTCAAGGCGGTGGGGGAAGAGGTGGTCGAGGCGGCGGTGGGAGAGATGGAGGTCGGGTGGATCATCCGGCTACACAGCAGTTTCCTTCTCCGACTCCTCAGCCTACTCCGGTTGCTTCCCGTCCTCCACTCGAGACATCTGCGGGAGCATCTTCTTCATCTTCTACAACTGCTGCTCTTTCACAGCCGGAGGCTTCTTCTTCGAGGATCGAGGCACAGTTGGAGAAGACATTGGTACTGCAGAATGTTCCGCCGTCTTCATCTAAGGCTATGACTGTGGCTAAGAGACCGGGATATGGAACCATTGGGCGCAAAGTTGTTGTTCGGGCTAATCATTTTCTGGTACAAGTTGAAGATAAAGATCTCTACCATTACGATGTAAGCCTCTATTTGTTAGTTTCGTCTAATCTTATGTTTTTTAGTGTTGACTCTCTGTCATTTAATTCATTGCTACAGAGAGGATCTTCCATACACTGCAATTAAGGCCCGCTCTTTATATATATATATTTTTTTCATTTCCTCCGAGTCGTGTTACATTCTTGGTTCTTTTTTGGTGTATTGTTTGTATTTGATATACGGACGTAATCTATTCTGGGAATTTTTTGTTCACGTTCGATCTTTGAAATATCCTCTTGAACTTCTTGATAACCATATGCACTGATTTGCGAGTGTTCTTAGTAATTTATTAGCTAGAGCATTCAGATGTATAATTATACTTTTAGGTTTCTATTACGCATGATGGTGGGAAAATTGTTTATGTTGCTGATCATCATCCATTTTCGTCCAAAGAGTTCATGATTAAATTAGTTTTGATATAATCTTTTCTGTTGAACTTTTGTTATCCACATTTGATCTTTTCAAGCGTCATCTTAAAATTTGTTATAACCATTTACTACTGGTTTGGGAGTGTTCTTAGTAACTTAATGGCTATATCATTCATACGTATAATTGTATTTATGGTTTTAGGTTTCTATTACACCTGAGGTTACATCCAAAAAGGTCTGCCGAGACATAATACACCAGCTTGCAAAAATGTACAAAGAATCTCATTTAGGTGGAAGATTTCTAGCATATGATGGTGGGAAAAGTGTTTATGTTGCTGGTGAACTTCCATTTGTGTCCAAAGAGTTTCTTATTAAATTAGTCCGTAATGATGATGCCGGGTCTTCTCAACCAAAAAGGTATGTCTTCAGTTACCTCGCATGTTTCTGTCCATGAATAAATAATCAATATTGAGTTTATCTGTCATTCACTATCTGTCATTCACTCTACAGGAAAGAACGTGAATTCAAAGTAACTATCAAATTTGCTTCCAAGCCAGACCTCAATCATCTACAACAGTTTTTACATGGACGGCAGCGAGATGCTCCTCAAGAAACTATCCAAGTTCTTGATGTAGTTCTGAGAACTACACCATCTATCCAGTTAGTTTATATGAAACTCCAATTACTCTATTTAGTTACACATGGATGGAAGTGATAACTATATATATTTTTTTTTTCCAGTTGCACAGTTGTTGGAAGGTCATTTTTCTCAACCGAGTTCGGAAAACCAGGTGAACTTGGTAATGGTGTTGAGTATTGGAGAGGATATTATCAAAGTCTGCGACCTGTACAAATGGGTCTATCTTTAAATATAGGTAACTCTTTGTTACTATCAATTGACTCTGTCACTGCTGCTCTTTTTAACGTTCATGAGTTCTGTACTGCAGATGTGTCAGCCAGATCATTTTATGAACCAATATATGTGACAGATTATATTGCTAAACATTTCAATTTGAGGAATTTGCGAACAGCAACTGATCAGGATTTCCGGAAGGTTATTGATACTGTCTGCGCATGTGATGTCTAAGAGTTTTGTAGTGGAGATTTCATAATATATAGCAGCTGTTTTTTTTTGCTTAATGTCAGATAAAAAAAGTACTCAGAGGTGTGAAGGTGGCATTAATGTGTGGGGAGCATGCCAGGACGTACAAAATTACTGGGATATCATCAGAGCCTGTAGACAGATTA

mRNA sequence

ATGTCTAGTGGGCGCGGTGGTCGTGGTCGTGGTGCCGGAGGTCGTGGAGGGCAGTCATCTCCGGCAATTTCTCGCGGTGGTGGTGGTAGAAGTGGCGGAGGTAGAGGTGGTCAAGGCGGTGGTGGAAGAGGTGGTCAAGGCGGTGGGGGAAGAGGTGGTCGAGGCGGCGGTGGGAGAGATGGAGGTCGGGTGGATCATCCGGCTACACAGCAGTTTCCTTCTCCGACTCCTCAGCCTACTCCGGTTGCTTCCCGTCCTCCACTCGAGACATCTGCGGGAGCATCTTCTTCATCTTCTACAACTGCTGCTCTTTCACAGCCGGAGGCTTCTTCTTCGAGGATCGAGGCACAGTTGGAGAAGACATTGGTACTGCAGAATGTTCCGCCGTCTTCATCTAAGGCTATGACTGTGGCTAAGAGACCGGGATATGGAACCATTGGGCGCAAAGTTGTTGTTCGGGCTAATCATTTTCTGGTACAAGTTGAAGATAAAGATCTCTACCATTACGATGTTTCTATTACACCTGAGGTTACATCCAAAAAGGTCTGCCGAGACATAATACACCAGCTTGCAAAAATGTACAAAGAATCTCATTTAGGTGGAAGATTTCTAGCATATGATGGTGGGAAAAGTGTTTATGTTGCTGGTGAACTTCCATTTGTGTCCAAAGAGTTTCTTATTAAATTAGTCCGTAATGATGATGCCGGGTCTTCTCAACCAAAAAGGAAAGAACGTGAATTCAAAGTAACTATCAAATTTGCTTCCAAGCCAGACCTCAATCATCTACAACAGTTTTTACATGGACGGCAGCGAGATGCTCCTCAAGAAACTATCCAAGTTCTTGATGTAGTTCTGAGAACTACACCATCTATCCATTGCACAGTTGTTGGAAGGTCATTTTTCTCAACCGAGTTCGGAAAACCAGGTGAACTTGGTAATGGTGTTGAGTATTGGAGAGGATATTATCAAAGTCTGCGACCTGTACAAATGGGTCTATCTTTAAATATAGATGTGTCAGCCAGATCATTTTATGAACCAATATATGTGACAGATTATATTGCTAAACATTTCAATTTGAGGAATTTGCGAACAGCAACTGATCAGGATTTCCGGAAGATAAAAAAAGTACTCAGAGGTGTGAAGGTGGCATTAATGTGTGGGGAGCATGCCAGGACGTACAAAATTACTGGGATATCATCAGAGCCTGTAGACAGATTA

Coding sequence (CDS)

ATGTCTAGTGGGCGCGGTGGTCGTGGTCGTGGTGCCGGAGGTCGTGGAGGGCAGTCATCTCCGGCAATTTCTCGCGGTGGTGGTGGTAGAAGTGGCGGAGGTAGAGGTGGTCAAGGCGGTGGTGGAAGAGGTGGTCAAGGCGGTGGGGGAAGAGGTGGTCGAGGCGGCGGTGGGAGAGATGGAGGTCGGGTGGATCATCCGGCTACACAGCAGTTTCCTTCTCCGACTCCTCAGCCTACTCCGGTTGCTTCCCGTCCTCCACTCGAGACATCTGCGGGAGCATCTTCTTCATCTTCTACAACTGCTGCTCTTTCACAGCCGGAGGCTTCTTCTTCGAGGATCGAGGCACAGTTGGAGAAGACATTGGTACTGCAGAATGTTCCGCCGTCTTCATCTAAGGCTATGACTGTGGCTAAGAGACCGGGATATGGAACCATTGGGCGCAAAGTTGTTGTTCGGGCTAATCATTTTCTGGTACAAGTTGAAGATAAAGATCTCTACCATTACGATGTTTCTATTACACCTGAGGTTACATCCAAAAAGGTCTGCCGAGACATAATACACCAGCTTGCAAAAATGTACAAAGAATCTCATTTAGGTGGAAGATTTCTAGCATATGATGGTGGGAAAAGTGTTTATGTTGCTGGTGAACTTCCATTTGTGTCCAAAGAGTTTCTTATTAAATTAGTCCGTAATGATGATGCCGGGTCTTCTCAACCAAAAAGGAAAGAACGTGAATTCAAAGTAACTATCAAATTTGCTTCCAAGCCAGACCTCAATCATCTACAACAGTTTTTACATGGACGGCAGCGAGATGCTCCTCAAGAAACTATCCAAGTTCTTGATGTAGTTCTGAGAACTACACCATCTATCCATTGCACAGTTGTTGGAAGGTCATTTTTCTCAACCGAGTTCGGAAAACCAGGTGAACTTGGTAATGGTGTTGAGTATTGGAGAGGATATTATCAAAGTCTGCGACCTGTACAAATGGGTCTATCTTTAAATATAGATGTGTCAGCCAGATCATTTTATGAACCAATATATGTGACAGATTATATTGCTAAACATTTCAATTTGAGGAATTTGCGAACAGCAACTGATCAGGATTTCCGGAAGATAAAAAAAGTACTCAGAGGTGTGAAGGTGGCATTAATGTGTGGGGAGCATGCCAGGACGTACAAAATTACTGGGATATCATCAGAGCCTGTAGACAGATTA

Protein sequence

MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRDGGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEKTLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSFFSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLRNLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Homology
BLAST of CmoCh13G002760 vs. ExPASy Swiss-Prot
Match: Q9SJK3 (Protein argonaute 5 OS=Arabidopsis thaliana OX=3702 GN=AGO5 PE=1 SV=2)

HSP 1 Score: 289.7 bits (740), Expect = 5.3e-77
Identity = 213/429 (49.65%), Postives = 259/429 (60.37%), Query Frame = 0

Query: 4   GRGGRGRGAGG------RGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGG- 63
           G GG  RG GG      R  QSS  ++  G  +S GGRGG    GR G+G  GRG   G 
Sbjct: 8   GHGGASRGRGGGRRSDQRQDQSSGQVAWPGLQQSYGGRGGSVSAGR-GRGNVGRGENTGD 67

Query: 64  -------------GGRDGGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAA 123
                        GGR  G +  P              VAS    +T + ASSS   +  
Sbjct: 68  LTATQVPVASAVSGGRGRGNIGDPTFS-----------VASSS--KTVSVASSSKEESKN 127

Query: 124 LSQPEASSSRIEAQLEKTLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVED 183
               E  S+      E    + ++PP+SSKA+T   RPG GT+G+KV+VRANHFLVQV D
Sbjct: 128 TEVSETMSNLQITSTETKPEMTSLPPASSKAVTFPVRPGRGTLGKKVMVRANHFLVQVAD 187

Query: 184 KDLYHYDVSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSK 243
           +DLYHYDVSI PEV SK V R+++  L K YK+SHLGG+  AYDG KS+Y AG LPF SK
Sbjct: 188 RDLYHYDVSINPEVISKTVNRNVMKLLVKNYKDSHLGGKSPAYDGRKSLYTAGPLPFDSK 247

Query: 244 EFLIKLVRNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDV 303
           EF++ L      GSS    K+R FKV +K  +  DL  LQQFL  +QR+AP +TIQVLDV
Sbjct: 248 EFVVNLAEKRADGSS---GKDRPFKVAVKNVTSTDLYQLQQFLDRKQREAPYDTIQVLDV 307

Query: 304 VLRTTPSIHCTVVGRSFFSTEFGKP-----GELGNGVEYWRGYYQSLRPVQMGLSLNIDV 363
           VLR  PS     VGRSFF T  GK      GELG+G+EYWRGY+QSLR  QMGLSLNIDV
Sbjct: 308 VLRDKPSNDYVSVGRSFFHTSLGKDARDGRGELGDGIEYWRGYFQSLRLTQMGLSLNIDV 367

Query: 364 SARSFYEPIYVTDYIAKHFNLRNL-RTATDQDFRKIKKVLRGVKVALMCGEHARTYKITG 407
           SARSFYEPI VTD+I+K  N+R+L R   D D  K+KKVLR +KV L+     ++ KI+G
Sbjct: 368 SARSFYEPIVVTDFISKFLNIRDLNRPLRDSDRLKVKKVLRTLKVKLLHWNGTKSAKISG 419

BLAST of CmoCh13G002760 vs. ExPASy Swiss-Prot
Match: Q6Z4F1 (Protein argonaute 14 OS=Oryza sativa subsp. japonica OX=39947 GN=AGO14 PE=2 SV=1)

HSP 1 Score: 269.2 bits (687), Expect = 7.4e-71
Identity = 205/454 (45.15%), Postives = 281/454 (61.89%), Query Frame = 0

Query: 4   GRGGRGRG-AGGRGG-----------QSSPAISRG-GGGR-----SGGGRG-GQGGGGRG 63
           GR GRGRG AGGRGG           Q  P   RG GGGR     +GG  G G GGGG G
Sbjct: 19  GRDGRGRGPAGGRGGGRGGGHPQQQQQQQPGYGRGDGGGRGPAPAAGGVVGRGTGGGGGG 78

Query: 64  GQGGGGRG-GRGGGGRDGGRVDHPATQQFP-SPTPQPTPVASR------PPLETSAGASS 123
           G+G GGRG GRGGGG DG R   PA    P + TP P  VA+R      P ++  A ASS
Sbjct: 79  GRGDGGRGRGRGGGGGDGVR---PAMAAAPAASTPGPVAVAARSTPPPTPAVQIPAVASS 138

Query: 124 SSSTTAALSQPEASSSRIEA---QLEKTLVL-----QNVPPS--------SSKAMTVAKR 183
           SS+  AA +QP  +++ + A    + + L +     +  PP+        SSK +    R
Sbjct: 139 SSAQPAAAAQPPPAAAAVSALARDVGRQLAVVAGGGRPAPPAAPPAPIPVSSKGVAPPSR 198

Query: 184 PGYGTIGRKVVVRANHFLVQVEDKD-LYHYDVSITPEVTSKKVCRDIIHQLAKMYKESHL 243
           PG+GT+G ++VVRANHFLV+V D D +Y YDVS++P   ++++ R ++ +LA++++ESHL
Sbjct: 199 PGFGTVGERIVVRANHFLVRVSDNDMIYLYDVSLSPPPKTRRINRVVMSELARLHRESHL 258

Query: 244 GGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQPKRKEREFKVTIKFASKPDL 303
           GG   AYDG K++Y AG+LPF S +F IKL +         + +E E+KVTI+ A + DL
Sbjct: 259 GGISFAYDGSKALYTAGKLPFDSMDFKIKLGK---------ELREIEYKVTIRRAGQADL 318

Query: 304 NHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSFFSTEFGKPGELGNGVEYWR 363
           +HL +F+ GRQRD+ Q+TIQ LDVVLR +PS++  +V RSF+ST FG+  ++G+G+E W+
Sbjct: 319 HHLHEFIAGRQRDSQQQTIQALDVVLRESPSLNYVIVSRSFYSTMFGRQ-DIGDGLECWK 378

Query: 364 GYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLRN-------LRTATDQDFRK 407
           GYYQSLRP QMGLSLNID+S+  F++PI V +Y+                R  +D D  K
Sbjct: 379 GYYQSLRPTQMGLSLNIDISSTPFFKPISVVEYVKNCLGTPTNANGPDPRRPLSDIDRLK 438

BLAST of CmoCh13G002760 vs. ExPASy Swiss-Prot
Match: Q851R2 (Protein argonaute MEL1 OS=Oryza sativa subsp. japonica OX=39947 GN=MEL1 PE=2 SV=1)

HSP 1 Score: 257.7 bits (657), Expect = 2.2e-67
Identity = 194/468 (41.45%), Postives = 258/468 (55.13%), Query Frame = 0

Query: 8   RGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGG-------------------------- 67
           RG G GGRGG+  P  S G G   G G GG GGG                          
Sbjct: 4   RGGGRGGRGGEQRPPYS-GRGDVPGRGGGGGGGGAPPYRPASGFVWPPPGMTPRPGPPQP 63

Query: 68  ----------------------GRGGQGGGGRGGRGGGGRDGGRV-DHPATQQFPSPTPQ 127
                                 G    GG  R    G    GG     P T + P P+  
Sbjct: 64  QYPRPGPPAVVYGAPMPAAHHQGAYQPGGVYRAPSPGVPVIGGYARSTPVTIRAPPPSHS 123

Query: 128 PTPVASRPPLETSAGASSSSSTTA----------------ALSQPEASSSRIEAQLEKTL 187
             P   +P     A +SSS++ +A                AL+ P A +S   A   +  
Sbjct: 124 SAPAPYQPAAAAPAPSSSSTAPSATALAKEFEQKLFVSETALAPPAAVASAAAAPAGEAS 183

Query: 188 VL--QNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 247
           V   +++ P S K +    RPG+G  G+KV++RANHFLV V D +L+HYDVSI PE  S+
Sbjct: 184 VESDKDLAPVSKKGLAHPARPGFGAAGKKVMIRANHFLVNVADNNLFHYDVSINPESKSR 243

Query: 248 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 307
              R+++++L K++ ++ LGG+  AYDG KS+Y AG LPF S+EF++KL+   D      
Sbjct: 244 ATNREVLNELIKLHGKTSLGGKLPAYDGRKSLYTAGSLPFESEEFVVKLI---DPEKKDK 303

Query: 308 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 367
           +R ERE+K+TI+ A + DL HLQQFL GRQRD PQETIQVLDVVLR +PS +   V RSF
Sbjct: 304 ERAEREYKITIRIAGRTDLYHLQQFLLGRQRDMPQETIQVLDVVLRESPSWNYVTVSRSF 363

Query: 368 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 407
           FST+FG  G++G G+E WRGYYQSLRP QMGLSLNID+SA SF++P+ V  ++ +  N+R
Sbjct: 364 FSTQFGHRGDIGEGLECWRGYYQSLRPTQMGLSLNIDISATSFFKPVTVIQFVEEFLNIR 423

BLAST of CmoCh13G002760 vs. ExPASy Swiss-Prot
Match: Q7Y001 (Protein argonaute 12 OS=Oryza sativa subsp. japonica OX=39947 GN=AGO12 PE=2 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 1.6e-65
Identity = 207/477 (43.40%), Postives = 263/477 (55.14%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQG-----------GGGRG-GQGG 60
           MSS  GG G   GGRGG        GGGG  GGGRGGQG           GGGRG G+ G
Sbjct: 1   MSSRGGGGGGRRGGRGGGGGR--EGGGGGGGGGGRGGQGRGDLGVVGERQGGGRGAGERG 60

Query: 61  G------GRGG---------------------RGGGGRDGGRVDHPATQQFP-------S 120
           G      GRGG                      GGGGR G +V   A  + P        
Sbjct: 61  GRHDAPRGRGGVAVGAGAGRQQQQPFHAPAPPSGGGGRGGVQVQPNAAARRPVGGGRGGV 120

Query: 121 PTPQPTPVAS-----------------RPPLETSAGASSSSSTTAALSQPEASSSRIEAQ 180
             P P P  +                  PP    +  +++  T     +P   ++   A 
Sbjct: 121 GVPAPAPAVAVGALCGEMKGKMVVSGGAPPAGQGSSLAAAQGTDNVKREPSQVAAPAPAP 180

Query: 181 LEKTLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEV 240
              TL     PPSSSKA+T   RP  GTIGR+  VRANHFLVQV DKD+YHYDV ITPE 
Sbjct: 181 PPATL-----PPSSSKAVTFPARPDVGTIGRRCRVRANHFLVQVADKDIYHYDVVITPES 240

Query: 241 TSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGS 300
           T ++  R II++L  ++K+  L GR   YDG KS+Y AG LPF +K+F++K +       
Sbjct: 241 TYRERNRSIINKLVALHKQ-FLDGRLPVYDGRKSIYTAGPLPFKTKDFVVKHI-----NP 300

Query: 301 SQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTP-SIHCTV- 360
            +  ++E E+KVTIK ASK DL  L+QFL GRQR+ PQ+TIQ LD+ LR  P S++ T  
Sbjct: 301 LRGNQREEEYKVTIKQASKTDLYSLKQFLVGRQRELPQDTIQALDIALRECPTSVNFTCD 360

Query: 361 ----VGRSFFSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTD 407
               + RSFFS  FG  GE+G+G E WRGYYQSLRP QMGLSLNID+SA +FY+   V D
Sbjct: 361 RYVSISRSFFSQSFGHGGEIGSGTECWRGYYQSLRPTQMGLSLNIDISATAFYKAQPVMD 420

BLAST of CmoCh13G002760 vs. ExPASy Swiss-Prot
Match: Q7XSA2 (Protein argonaute 1B OS=Oryza sativa subsp. japonica OX=39947 GN=AGO1B PE=2 SV=3)

HSP 1 Score: 251.1 bits (640), Expect = 2.1e-65
Identity = 195/454 (42.95%), Postives = 255/454 (56.17%), Query Frame = 0

Query: 4   GRGGRGRGAGGRG------------GQSSPAISRGGGGRSG-GGRGGQG----GGG---- 63
           GRGG  +G GG+G            G+  P     GGG     GRGG G    GGG    
Sbjct: 75  GRGGHYQGRGGQGSHHPGGGPPEYQGRGGPGSHHPGGGPPDYQGRGGSGSHHPGGGPPEY 134

Query: 64  --RGGQGGGG---RGG---------RGGGGR---DGGRVDHPATQQFP---------SPT 123
             R  QG GG   RGG         RG GGR    G     P   Q P         SPT
Sbjct: 135 QPRDYQGRGGPRPRGGMPQPYYGGPRGSGGRSVPSGSSRTVPELHQAPHVQYQAPMVSPT 194

Query: 124 PQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEKTLVLQNVPPSSSKAMT 183
           P     +S+P  E S+G         A     ++S  I+           + P SSK++ 
Sbjct: 195 PSGAGSSSQPAAEVSSGQVQQQFQQLATRDQSSTSQAIQ-----------IAPPSSKSVR 254

Query: 184 VAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSKKVCRDIIHQLAKMYKE 243
              RPG GT G + +V+ANHF  ++ DKDL+ YDVSITPEVTS+ V R ++ +L  +Y+ 
Sbjct: 255 FPLRPGKGTYGDRCIVKANHFFAELPDKDLHQYDVSITPEVTSRGVNRAVMFELVTLYRY 314

Query: 244 SHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDA--GSSQPKRKEREFKVTIKFA 303
           SHLGGR  AYDG KS+Y AG LPF S+ F I L   +D+  G    +R+ER F+V IKFA
Sbjct: 315 SHLGGRLPAYDGRKSLYTAGPLPFASRTFEITLQDEEDSLGGGQGTQRRERLFRVVIKFA 374

Query: 304 SKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSFFSTEFGKPGELGNG 363
           ++ DL+HL  FL GRQ DAPQE +QVLD+VLR  P+   + VGRSF+S   G+  +LG G
Sbjct: 375 ARADLHHLAMFLAGRQADAPQEALQVLDIVLRELPTTRYSPVGRSFYSPNLGRRQQLGEG 434

Query: 364 VEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR-NLRTATDQDFRKI 407
           +E WRG+YQS+RP QMGLSLNID+S+ +F EP+ V D++A+  N   ++R  +D D  KI
Sbjct: 435 LESWRGFYQSIRPTQMGLSLNIDMSSTAFIEPLPVIDFVAQLLNRDISVRPLSDSDRVKI 494

BLAST of CmoCh13G002760 vs. ExPASy TrEMBL
Match: A0A6J1HIR7 (protein argonaute 5-like OS=Cucurbita moschata OX=3662 GN=LOC111464815 PE=4 SV=1)

HSP 1 Score: 756.9 bits (1953), Expect = 4.3e-215
Identity = 406/406 (100.00%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60
           MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD
Sbjct: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60

Query: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120
           GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK
Sbjct: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120

Query: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180
           TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK
Sbjct: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180

Query: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240
           KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP
Sbjct: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240

Query: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300
           KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF
Sbjct: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300

Query: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360
           FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR
Sbjct: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360

Query: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Sbjct: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 406

BLAST of CmoCh13G002760 vs. ExPASy TrEMBL
Match: A0A6J1HLM7 (protein argonaute 5 OS=Cucurbita moschata OX=3662 GN=LOC111464716 PE=3 SV=1)

HSP 1 Score: 754.2 bits (1946), Expect = 2.8e-214
Identity = 404/406 (99.51%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60
           MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD
Sbjct: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60

Query: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120
           GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK
Sbjct: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120

Query: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180
           TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK
Sbjct: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180

Query: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240
           KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP
Sbjct: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240

Query: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300
           +RKEREFKVTIKFASKPDLNHL+QFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF
Sbjct: 241 RRKEREFKVTIKFASKPDLNHLRQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300

Query: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360
           FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR
Sbjct: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360

Query: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Sbjct: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 406

BLAST of CmoCh13G002760 vs. ExPASy TrEMBL
Match: A0A6J1I537 (protein argonaute 5 OS=Cucurbita maxima OX=3661 GN=LOC111469299 PE=3 SV=1)

HSP 1 Score: 745.7 bits (1924), Expect = 9.9e-212
Identity = 399/406 (98.28%), Postives = 402/406 (99.01%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60
           MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD
Sbjct: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60

Query: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120
           GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK
Sbjct: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120

Query: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180
           TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSI+PEVTSK
Sbjct: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSISPEVTSK 180

Query: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240
           KVCRDIIHQLA MYKESHLGGRFLAYDGGKSVYVAGELPF SKEF+I LVRNDD GSSQP
Sbjct: 181 KVCRDIIHQLANMYKESHLGGRFLAYDGGKSVYVAGELPFSSKEFIINLVRNDDTGSSQP 240

Query: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300
           +RKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF
Sbjct: 241 RRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300

Query: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360
           FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR
Sbjct: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360

Query: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Sbjct: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 406

BLAST of CmoCh13G002760 vs. ExPASy TrEMBL
Match: A0A1S3CGI6 (protein argonaute 5 OS=Cucumis melo OX=3656 GN=LOC103500648 PE=3 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 4.7e-161
Identity = 323/417 (77.46%), Postives = 350/417 (83.93%), Query Frame = 0

Query: 3   SGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRDGG 62
           S RGG GRG+GGRGGQ SPA+SRGGGG S             G+GGGGRGGRGGGGRDGG
Sbjct: 2   SRRGGGGRGSGGRGGQPSPAVSRGGGGGS-------------GRGGGGRGGRGGGGRDGG 61

Query: 63  RVDHPATQQFPSPT----------PQPTPVASRPPLETSAGASS-SSSTTAALSQPEASS 122
           RV HP TQQF SP+          PQPTPVAS PP +TS G SS SSS TAALSQPE   
Sbjct: 62  RVGHPTTQQFHSPSPQPTPVVSAPPQPTPVASPPPPQTSTGTSSASSSGTAALSQPEV-- 121

Query: 123 SRIEAQLEK-TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYD 182
           S+IEAQ+EK TL  QNVPPSSSK +TVAKRPGYGT GRKVVVRANHFLVQV DKDLYHYD
Sbjct: 122 SKIEAQVEKMTLTPQNVPPSSSKDLTVAKRPGYGTAGRKVVVRANHFLVQVADKDLYHYD 181

Query: 183 VSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLV 242
           VSITPEV SK+VCRDII+QLA  Y+ESHLGGR+LAYDGGKSVYVAG+LPF SKEF+IKLV
Sbjct: 182 VSITPEVASKRVCRDIINQLANTYRESHLGGRYLAYDGGKSVYVAGQLPFSSKEFMIKLV 241

Query: 243 RNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPS 302
           R D AGSSQP R+EREFKV+IKFASKPDL+HLQQF+HGRQRDAPQETIQVLDVVLRT PS
Sbjct: 242 RKDGAGSSQPTRREREFKVSIKFASKPDLHHLQQFIHGRQRDAPQETIQVLDVVLRTKPS 301

Query: 303 IHCTVVGRSFFSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVT 362
           +  TVVGRSFF+ E G+PG+LGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPI VT
Sbjct: 302 VEYTVVGRSFFALELGEPGDLGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPICVT 361

Query: 363 DYIAKHFNLRNL-RTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           +Y+ KHFNLRN  R  +DQD RKI+KVLRGVKV L C EHARTYKITGISSEPV+RL
Sbjct: 362 EYVVKHFNLRNFSRPMSDQDCRKIRKVLRGVKVGLTCREHARTYKITGISSEPVNRL 403

BLAST of CmoCh13G002760 vs. ExPASy TrEMBL
Match: A0A5A7UF40 (Protein argonaute 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold578G00660 PE=3 SV=1)

HSP 1 Score: 573.5 bits (1477), Expect = 6.8e-160
Identity = 321/417 (76.98%), Postives = 348/417 (83.45%), Query Frame = 0

Query: 3   SGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRDGG 62
           S RGG GRG+GGRGGQ SPA+SRGGGG S             G+GGGGRGGRGGGGRDGG
Sbjct: 2   SRRGGGGRGSGGRGGQPSPAVSRGGGGGS-------------GRGGGGRGGRGGGGRDGG 61

Query: 63  RVDHPATQQFPSPT----------PQPTPVASRPPLETSAGASS-SSSTTAALSQPEASS 122
           RV HP TQQF SP+          PQPTPVAS PP +TS G SS SSS TAALSQPE   
Sbjct: 62  RVGHPTTQQFHSPSPQPTPVVSAPPQPTPVASPPPPQTSTGTSSASSSGTAALSQPEV-- 121

Query: 123 SRIEAQLEK-TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYD 182
           S+IEAQ+EK TL  QNVPPSSSK +TVAKRPGYGT GRKVVVRANHFLVQV DKDLYHYD
Sbjct: 122 SKIEAQVEKMTLTPQNVPPSSSKDLTVAKRPGYGTAGRKVVVRANHFLVQVADKDLYHYD 181

Query: 183 VSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLV 242
           VSITPEV SK+VCRDII+QLA  Y+ESHLGGR+LAYDGGKSVYVAG+LPF SKEF+IKLV
Sbjct: 182 VSITPEVASKRVCRDIINQLANTYRESHLGGRYLAYDGGKSVYVAGQLPFSSKEFMIKLV 241

Query: 243 RNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPS 302
           R D AGSSQP R+EREFKV+IKFASKPDL+HLQQF+HGRQRDAPQETIQVLDVVLRT PS
Sbjct: 242 RKDGAGSSQPTRREREFKVSIKFASKPDLHHLQQFIHGRQRDAPQETIQVLDVVLRTKPS 301

Query: 303 IHCTVVGRSFFSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVT 362
           +  TVVGRSFF+ E G+PG+LGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPI VT
Sbjct: 302 VEYTVVGRSFFALELGEPGDLGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPICVT 361

Query: 363 DYIAKHFNLRNL-RTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           +Y+ KHFNLRN  R  +DQD RKI+KVLRGVKV L C EHARTYKITGISSEP  R+
Sbjct: 362 EYVVKHFNLRNFSRPMSDQDCRKIRKVLRGVKVGLTCREHARTYKITGISSEPKTRI 403

BLAST of CmoCh13G002760 vs. NCBI nr
Match: XP_022964827.1 (protein argonaute 5-like [Cucurbita moschata])

HSP 1 Score: 756.9 bits (1953), Expect = 8.9e-215
Identity = 406/406 (100.00%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60
           MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD
Sbjct: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60

Query: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120
           GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK
Sbjct: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120

Query: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180
           TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK
Sbjct: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180

Query: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240
           KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP
Sbjct: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240

Query: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300
           KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF
Sbjct: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300

Query: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360
           FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR
Sbjct: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360

Query: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Sbjct: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 406

BLAST of CmoCh13G002760 vs. NCBI nr
Match: XP_023519443.1 (protein argonaute 5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755.7 bits (1950), Expect = 2.0e-214
Identity = 405/406 (99.75%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60
           MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD
Sbjct: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60

Query: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120
           GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK
Sbjct: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120

Query: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180
           TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK
Sbjct: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180

Query: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240
           KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP
Sbjct: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240

Query: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300
           KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF
Sbjct: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300

Query: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360
           FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYI+KHFNLR
Sbjct: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYISKHFNLR 360

Query: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Sbjct: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 406

BLAST of CmoCh13G002760 vs. NCBI nr
Match: XP_022964725.1 (protein argonaute 5 [Cucurbita moschata])

HSP 1 Score: 754.2 bits (1946), Expect = 5.8e-214
Identity = 404/406 (99.51%), Postives = 406/406 (100.00%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60
           MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD
Sbjct: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60

Query: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120
           GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK
Sbjct: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120

Query: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180
           TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK
Sbjct: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180

Query: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240
           KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP
Sbjct: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240

Query: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300
           +RKEREFKVTIKFASKPDLNHL+QFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF
Sbjct: 241 RRKEREFKVTIKFASKPDLNHLRQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300

Query: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360
           FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR
Sbjct: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360

Query: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Sbjct: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 406

BLAST of CmoCh13G002760 vs. NCBI nr
Match: KAG6583598.1 (Protein argonaute 5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 750.7 bits (1937), Expect = 6.4e-213
Identity = 403/406 (99.26%), Postives = 405/406 (99.75%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60
           MSS RGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD
Sbjct: 1   MSSVRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60

Query: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120
           GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK
Sbjct: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120

Query: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180
           TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK
Sbjct: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180

Query: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240
           KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP
Sbjct: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240

Query: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300
           +RKEREFKVTIKFASKPDLNHL+QFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF
Sbjct: 241 RRKEREFKVTIKFASKPDLNHLRQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300

Query: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360
           FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR
Sbjct: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360

Query: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Sbjct: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 406

BLAST of CmoCh13G002760 vs. NCBI nr
Match: XP_022970289.1 (protein argonaute 5 [Cucurbita maxima])

HSP 1 Score: 745.7 bits (1924), Expect = 2.1e-211
Identity = 399/406 (98.28%), Postives = 402/406 (99.01%), Query Frame = 0

Query: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60
           MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD
Sbjct: 1   MSSGRGGRGRGAGGRGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGGGGRD 60

Query: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120
           GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK
Sbjct: 61  GGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRIEAQLEK 120

Query: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSITPEVTSK 180
           TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSI+PEVTSK
Sbjct: 121 TLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYDVSISPEVTSK 180

Query: 181 KVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLVRNDDAGSSQP 240
           KVCRDIIHQLA MYKESHLGGRFLAYDGGKSVYVAGELPF SKEF+I LVRNDD GSSQP
Sbjct: 181 KVCRDIIHQLANMYKESHLGGRFLAYDGGKSVYVAGELPFSSKEFIINLVRNDDTGSSQP 240

Query: 241 KRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300
           +RKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF
Sbjct: 241 RRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPSIHCTVVGRSF 300

Query: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360
           FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR
Sbjct: 301 FSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVTDYIAKHFNLR 360

Query: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 407
           NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL
Sbjct: 361 NLRTATDQDFRKIKKVLRGVKVALMCGEHARTYKITGISSEPVDRL 406

BLAST of CmoCh13G002760 vs. TAIR 10
Match: AT2G27880.1 (Argonaute family protein )

HSP 1 Score: 289.7 bits (740), Expect = 3.8e-78
Identity = 213/429 (49.65%), Postives = 259/429 (60.37%), Query Frame = 0

Query: 4   GRGGRGRGAGG------RGGQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGG- 63
           G GG  RG GG      R  QSS  ++  G  +S GGRGG    GR G+G  GRG   G 
Sbjct: 8   GHGGASRGRGGGRRSDQRQDQSSGQVAWPGLQQSYGGRGGSVSAGR-GRGNVGRGENTGD 67

Query: 64  -------------GGRDGGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAA 123
                        GGR  G +  P              VAS    +T + ASSS   +  
Sbjct: 68  LTATQVPVASAVSGGRGRGNIGDPTFS-----------VASSS--KTVSVASSSKEESKN 127

Query: 124 LSQPEASSSRIEAQLEKTLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVED 183
               E  S+      E    + ++PP+SSKA+T   RPG GT+G+KV+VRANHFLVQV D
Sbjct: 128 TEVSETMSNLQITSTETKPEMTSLPPASSKAVTFPVRPGRGTLGKKVMVRANHFLVQVAD 187

Query: 184 KDLYHYDVSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSK 243
           +DLYHYDVSI PEV SK V R+++  L K YK+SHLGG+  AYDG KS+Y AG LPF SK
Sbjct: 188 RDLYHYDVSINPEVISKTVNRNVMKLLVKNYKDSHLGGKSPAYDGRKSLYTAGPLPFDSK 247

Query: 244 EFLIKLVRNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDV 303
           EF++ L      GSS    K+R FKV +K  +  DL  LQQFL  +QR+AP +TIQVLDV
Sbjct: 248 EFVVNLAEKRADGSS---GKDRPFKVAVKNVTSTDLYQLQQFLDRKQREAPYDTIQVLDV 307

Query: 304 VLRTTPSIHCTVVGRSFFSTEFGKP-----GELGNGVEYWRGYYQSLRPVQMGLSLNIDV 363
           VLR  PS     VGRSFF T  GK      GELG+G+EYWRGY+QSLR  QMGLSLNIDV
Sbjct: 308 VLRDKPSNDYVSVGRSFFHTSLGKDARDGRGELGDGIEYWRGYFQSLRLTQMGLSLNIDV 367

Query: 364 SARSFYEPIYVTDYIAKHFNLRNL-RTATDQDFRKIKKVLRGVKVALMCGEHARTYKITG 407
           SARSFYEPI VTD+I+K  N+R+L R   D D  K+KKVLR +KV L+     ++ KI+G
Sbjct: 368 SARSFYEPIVVTDFISKFLNIRDLNRPLRDSDRLKVKKVLRTLKVKLLHWNGTKSAKISG 419

BLAST of CmoCh13G002760 vs. TAIR 10
Match: AT1G48410.1 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 247.3 bits (630), Expect = 2.1e-65
Identity = 190/415 (45.78%), Postives = 246/415 (59.28%), Query Frame = 0

Query: 5   RGGRG-------RGAGGRG-GQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGG 64
           +GGRG        G GGRG GQ      + GG +   GRG  G   +GG+GG G GGRGG
Sbjct: 45  QGGRGYTPQPQQGGRGGRGYGQPPQQQQQYGGPQEYQGRGRGGPPHQGGRGGYG-GGRGG 104

Query: 65  GGRDG--GRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRI 124
           G   G   R   P   Q  SPT Q   V+S+P L          S  +    PE +   +
Sbjct: 105 GPSSGPPQRQSVPELHQATSPTYQ--AVSSQPTL----------SEVSPTQVPEPTV--L 164

Query: 125 EAQLEKTLVLQNVP-------PSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLY 184
             Q E+  V Q  P       PSSSKA     RPG G  G++ +V+ANHF  ++ DKDL+
Sbjct: 165 AQQFEQLSVEQGAPSQAIQPIPSSSKAFKFPMRPGKGQSGKRCIVKANHFFAELPDKDLH 224

Query: 185 HYDVSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLI 244
           HYDV+ITPEVTS+ V R ++ QL   Y++SHLG R  AYDG KS+Y AG LPF SKEF I
Sbjct: 225 HYDVTITPEVTSRGVNRAVMKQLVDNYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRI 284

Query: 245 KLVRNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRT 304
            L+  D+   +  +R+EREFKV IK  ++ DL+HL  FL G+Q DAPQE +QVLD+VLR 
Sbjct: 285 NLL--DEEVGAGGQRREREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRE 344

Query: 305 TPSIHCTVVGRSFFSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPI 364
            P+     VGRSF+S + GK   LG+G+E WRG+YQS+RP QMGLSLNID+S+ +F E  
Sbjct: 345 LPTSRYIPVGRSFYSPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIEAN 404

Query: 365 YVTDYIAKHFNLR-NLRTATDQDFRKIKKVLRGVKVALM-CGEHARTYKITGISS 401
            V  ++    N   + R  +D D  KIKK LRGVKV +   G   R Y+I+G+++
Sbjct: 405 PVIQFVCDLLNRDISSRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTA 442

BLAST of CmoCh13G002760 vs. TAIR 10
Match: AT5G43810.1 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 246.1 bits (627), Expect = 4.8e-65
Identity = 154/358 (43.02%), Postives = 214/358 (59.78%), Query Frame = 0

Query: 51  RGGRGGGGRDGGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEAS 110
           R  RGG   D G V        PS  P+  P    PP +T    +SS+ + A   +  A 
Sbjct: 68  RRNRGGRKSDQGDV-----CMRPSSRPRKPP----PPSQT----TSSAVSVATAGEIVAV 127

Query: 111 SSRIEAQLEKTLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYD 170
           + +++  + K                 A RPG+GT+G K +V+ANHFL  +  KDL  YD
Sbjct: 128 NHQMQMGVRKN-------------SNFAPRPGFGTLGTKCIVKANHFLADLPTKDLNQYD 187

Query: 171 VSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLV 230
           V+ITPEV+SK V R II +L ++YKES LG R  AYDG KS+Y AGELPF  KEF +K+V
Sbjct: 188 VTITPEVSSKSVNRAIIAELVRLYKESDLGRRLPAYDGRKSLYTAGELPFTWKEFSVKIV 247

Query: 231 RNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPS 290
             DD   + PKR ER +KV IKF ++ +++HL +FL G++ D PQE +Q+LD+VLR    
Sbjct: 248 DEDDGIINGPKR-ERSYKVAIKFVARANMHHLGEFLAGKRADCPQEAVQILDIVLRELSV 307

Query: 291 IHCTVVGRSFFSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVT 350
                VGRSFFS +   P  LG G+E W G+YQS+RP QMGLSLNID+++ +F EP+ V 
Sbjct: 308 KRFCPVGRSFFSPDIKTPQRLGEGLESWCGFYQSIRPTQMGLSLNIDMASAAFIEPLPVI 367

Query: 351 DYIAKHFNLRNL-RTATDQDFRKIKKVLRGVKVALMCGEHA-RTYKITGISSEPVDRL 407
           +++A+      L +  +D D  KIKK LRGVKV +    +  R Y++ G++++P   L
Sbjct: 368 EFVAQLLGKDVLSKPLSDSDRVKIKKGLRGVKVEVTHRANVRRKYRVAGLTTQPTREL 398

BLAST of CmoCh13G002760 vs. TAIR 10
Match: AT5G43810.2 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 246.1 bits (627), Expect = 4.8e-65
Identity = 154/358 (43.02%), Postives = 214/358 (59.78%), Query Frame = 0

Query: 51  RGGRGGGGRDGGRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEAS 110
           R  RGG   D G V        PS  P+  P    PP +T    +SS+ + A   +  A 
Sbjct: 68  RRNRGGRKSDQGDV-----CMRPSSRPRKPP----PPSQT----TSSAVSVATAGEIVAV 127

Query: 111 SSRIEAQLEKTLVLQNVPPSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLYHYD 170
           + +++  + K                 A RPG+GT+G K +V+ANHFL  +  KDL  YD
Sbjct: 128 NHQMQMGVRKN-------------SNFAPRPGFGTLGTKCIVKANHFLADLPTKDLNQYD 187

Query: 171 VSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLIKLV 230
           V+ITPEV+SK V R II +L ++YKES LG R  AYDG KS+Y AGELPF  KEF +K+V
Sbjct: 188 VTITPEVSSKSVNRAIIAELVRLYKESDLGRRLPAYDGRKSLYTAGELPFTWKEFSVKIV 247

Query: 231 RNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRTTPS 290
             DD   + PKR ER +KV IKF ++ +++HL +FL G++ D PQE +Q+LD+VLR    
Sbjct: 248 DEDDGIINGPKR-ERSYKVAIKFVARANMHHLGEFLAGKRADCPQEAVQILDIVLRELSV 307

Query: 291 IHCTVVGRSFFSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYEPIYVT 350
                VGRSFFS +   P  LG G+E W G+YQS+RP QMGLSLNID+++ +F EP+ V 
Sbjct: 308 KRFCPVGRSFFSPDIKTPQRLGEGLESWCGFYQSIRPTQMGLSLNIDMASAAFIEPLPVI 367

Query: 351 DYIAKHFNLRNL-RTATDQDFRKIKKVLRGVKVALMCGEHA-RTYKITGISSEPVDRL 407
           +++A+      L +  +D D  KIKK LRGVKV +    +  R Y++ G++++P   L
Sbjct: 368 EFVAQLLGKDVLSKPLSDSDRVKIKKGLRGVKVEVTHRANVRRKYRVAGLTTQPTREL 398

BLAST of CmoCh13G002760 vs. TAIR 10
Match: AT1G48410.2 (Stabilizer of iron transporter SufD / Polynucleotidyl transferase )

HSP 1 Score: 244.6 bits (623), Expect = 1.4e-64
Identity = 191/417 (45.80%), Postives = 247/417 (59.23%), Query Frame = 0

Query: 5   RGGRG-------RGAGGRG-GQSSPAISRGGGGRSGGGRGGQGGGGRGGQGGGGRGGRGG 64
           +GGRG        G GGRG GQ      + GG +   GRG  G   +GG+GG G GGRGG
Sbjct: 45  QGGRGYTPQPQQGGRGGRGYGQPPQQQQQYGGPQEYQGRGRGGPPHQGGRGGYG-GGRGG 104

Query: 65  GGRDG--GRVDHPATQQFPSPTPQPTPVASRPPLETSAGASSSSSTTAALSQPEASSSRI 124
           G   G   R   P   Q  SPT Q   V+S+P L          S  +    PE +   +
Sbjct: 105 GPSSGPPQRQSVPELHQATSPTYQ--AVSSQPTL----------SEVSPTQVPEPTV--L 164

Query: 125 EAQLEKTLVLQNVP-------PSSSKAMTVAKRPGYGTIGRKVVVRANHFLVQVEDKDLY 184
             Q E+  V Q  P       PSSSKA     RPG G  G++ +V+ANHF  ++ DKDL+
Sbjct: 165 AQQFEQLSVEQGAPSQAIQPIPSSSKAFKFPMRPGKGQSGKRCIVKANHFFAELPDKDLH 224

Query: 185 HYDVSITPEVTSKKVCRDIIHQLAKMYKESHLGGRFLAYDGGKSVYVAGELPFVSKEFLI 244
           HYDV+ITPEVTS+ V R ++ QL   Y++SHLG R  AYDG KS+Y AG LPF SKEF I
Sbjct: 225 HYDVTITPEVTSRGVNRAVMKQLVDNYRDSHLGSRLPAYDGRKSLYTAGPLPFNSKEFRI 284

Query: 245 KLVRNDDAGSSQPKRKEREFKVTIKFASKPDLNHLQQFLHGRQRDAPQETIQVLDVVLRT 304
            L+  D+   +  +R+EREFKV IK  ++ DL+HL  FL G+Q DAPQE +QVLD+VLR 
Sbjct: 285 NLL--DEEVGAGGQRREREFKVVIKLVARADLHHLGMFLEGKQSDAPQEALQVLDIVLRE 344

Query: 305 TPS--IHCTVVGRSFFSTEFGKPGELGNGVEYWRGYYQSLRPVQMGLSLNIDVSARSFYE 364
            P+  I    VGRSF+S + GK   LG+G+E WRG+YQS+RP QMGLSLNID+S+ +F E
Sbjct: 345 LPTSRIRYIPVGRSFYSPDIGKKQSLGDGLESWRGFYQSIRPTQMGLSLNIDMSSTAFIE 404

Query: 365 PIYVTDYIAKHFNLR-NLRTATDQDFRKIKKVLRGVKVALM-CGEHARTYKITGISS 401
              V  ++    N   + R  +D D  KIKK LRGVKV +   G   R Y+I+G+++
Sbjct: 405 ANPVIQFVCDLLNRDISSRPLSDADRVKIKKALRGVKVEVTHRGNMRRKYRISGLTA 444

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SJK35.3e-7749.65Protein argonaute 5 OS=Arabidopsis thaliana OX=3702 GN=AGO5 PE=1 SV=2[more]
Q6Z4F17.4e-7145.15Protein argonaute 14 OS=Oryza sativa subsp. japonica OX=39947 GN=AGO14 PE=2 SV=1[more]
Q851R22.2e-6741.45Protein argonaute MEL1 OS=Oryza sativa subsp. japonica OX=39947 GN=MEL1 PE=2 SV=... [more]
Q7Y0011.6e-6543.40Protein argonaute 12 OS=Oryza sativa subsp. japonica OX=39947 GN=AGO12 PE=2 SV=2[more]
Q7XSA22.1e-6542.95Protein argonaute 1B OS=Oryza sativa subsp. japonica OX=39947 GN=AGO1B PE=2 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1HIR74.3e-215100.00protein argonaute 5-like OS=Cucurbita moschata OX=3662 GN=LOC111464815 PE=4 SV=1[more]
A0A6J1HLM72.8e-21499.51protein argonaute 5 OS=Cucurbita moschata OX=3662 GN=LOC111464716 PE=3 SV=1[more]
A0A6J1I5379.9e-21298.28protein argonaute 5 OS=Cucurbita maxima OX=3661 GN=LOC111469299 PE=3 SV=1[more]
A0A1S3CGI64.7e-16177.46protein argonaute 5 OS=Cucumis melo OX=3656 GN=LOC103500648 PE=3 SV=1[more]
A0A5A7UF406.8e-16076.98Protein argonaute 5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold578G... [more]
Match NameE-valueIdentityDescription
XP_022964827.18.9e-215100.00protein argonaute 5-like [Cucurbita moschata][more]
XP_023519443.12.0e-21499.75protein argonaute 5 [Cucurbita pepo subsp. pepo][more]
XP_022964725.15.8e-21499.51protein argonaute 5 [Cucurbita moschata][more]
KAG6583598.16.4e-21399.26Protein argonaute 5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022970289.12.1e-21198.28protein argonaute 5 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
AT2G27880.13.8e-7849.65Argonaute family protein [more]
AT1G48410.12.1e-6545.78Stabilizer of iron transporter SufD / Polynucleotidyl transferase [more]
AT5G43810.14.8e-6543.02Stabilizer of iron transporter SufD / Polynucleotidyl transferase [more]
AT5G43810.24.8e-6543.02Stabilizer of iron transporter SufD / Polynucleotidyl transferase [more]
AT1G48410.21.4e-6445.80Stabilizer of iron transporter SufD / Polynucleotidyl transferase [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR014811Argonaute, linker 1 domainSMARTSM01163DUF1785_2coord: 294..346
e-value: 2.5E-25
score: 100.2
IPR014811Argonaute, linker 1 domainPFAMPF08699ArgoL1coord: 296..345
e-value: 8.0E-20
score: 70.2
IPR032474Protein argonaute, N-terminalPFAMPF16486ArgoNcoord: 148..285
e-value: 2.0E-33
score: 115.8
NoneNo IPR availableGENE3D2.170.260.10paz domaincoord: 341..406
e-value: 3.6E-7
score: 32.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..112
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..112
NoneNo IPR availablePANTHERPTHR22891:SF139PROTEIN ARGONAUTE 1Acoord: 47..401
NoneNo IPR availablePANTHERPTHR22891EUKARYOTIC TRANSLATION INITIATION FACTOR 2Ccoord: 47..401
IPR036085PAZ domain superfamilySUPERFAMILY101690PAZ domaincoord: 149..402

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh13G002760.1CmoCh13G002760.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051607 defense response to virus
biological_process GO:0031047 gene silencing by RNA
biological_process GO:0006955 immune response
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0005515 protein binding