CmoCh10G002550 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh10G002550
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
Location	Cmo_Chr10: 1131405 .. 1132147 (-)
RNA-Seq Expression	CmoCh10G002550
Synteny	CmoCh10G002550

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCATCTCTTCCATATCTCCCATGCCTAACCTTGCTCTTTCTCCTGCACGTTCTTCTACCGATTGCTGTTTCACAAGATCCGACCCTCTCGAGCCCCACCATAGCTCAATGCACCGCTAGTCTTCTTCCATTGGCATCGTGTGCGCCTTTCATGCAAGGTATGGCGTCAATGCCACCAATGACATGCTGTGGTAACCTGAAACAACTCTACACCAGTGTCCCCAGCTGCCTTTGCCTCTTGCTGAATGGCACTACCTTGAGTTCTTTCCCTATCAACACTACTAGAGCTACCCAGCTGCCTGATCTTTGCAGCCTTCAAGTTAACATCTCTACATGTTCAGGTATCACTATAATCCTGTCATCCTAAAGCCTCTTAAGTATCTTCTTCTACTTCGTTCTAACATTTATAACTCTCTGAATGGTCATTTGAAGCTCTCCTTGAGCCACCAAGTCCTCCAAGTTCTCATGTTTCTCCTAGAGTAGACACCAACACCACAATTACCAATGCTACGGTTCTAGGTATAGTTTTTGGTTCAGCTCTACTACAAGCACGACATGTTATACATTGTGACACTACTTTTATGGATTTCAGCTACTCCAGTGGGTCAACCAACGCCAAAACCTAGCATGGTGGGGTTAGGATTGGGCAGCATCAGTGCTAGTTTCAAGCCAAGGGCGGGAATTCAGCTCACAGTAGTGATTGCTATGAAGACTTTTCTGTTTCTTCTAGTTCTTCATTGA

mRNA sequence

ATGGCATCTCTTCCATATCTCCCATGCCTAACCTTGCTCTTTCTCCTGCACGTTCTTCTACCGATTGCTGTTTCACAAGATCCGACCCTCTCGAGCCCCACCATAGCTCAATGCACCGCTAGTCTTCTTCCATTGGCATCGTGTGCGCCTTTCATGCAAGGTATGGCGTCAATGCCACCAATGACATGCTGTGGTAACCTGAAACAACTCTACACCAGTGTCCCCAGCTGCCTTTGCCTCTTGCTGAATGGCACTACCTTGAGTTCTTTCCCTATCAACACTACTAGAGCTACCCAGCTGCCTGATCTTTGCAGCCTTCAAGTTAACATCTCTACATGTTCAGCTCTCCTTGAGCCACCAAGTCCTCCAAGTTCTCATGTTTCTCCTAGAGTAGACACCAACACCACAATTACCAATGCTACGGTTCTAGCTACTCCAGTGGGTCAACCAACGCCAAAACCTAGCATGGTGGGGTTAGGATTGGGCAGCATCAGTGCTAGTTTCAAGCCAAGGGCGGGAATTCAGCTCACAGTAGTGATTGCTATGAAGACTTTTCTGTTTCTTCTAGTTCTTCATTGA

Coding sequence (CDS)

ATGGCATCTCTTCCATATCTCCCATGCCTAACCTTGCTCTTTCTCCTGCACGTTCTTCTACCGATTGCTGTTTCACAAGATCCGACCCTCTCGAGCCCCACCATAGCTCAATGCACCGCTAGTCTTCTTCCATTGGCATCGTGTGCGCCTTTCATGCAAGGTATGGCGTCAATGCCACCAATGACATGCTGTGGTAACCTGAAACAACTCTACACCAGTGTCCCCAGCTGCCTTTGCCTCTTGCTGAATGGCACTACCTTGAGTTCTTTCCCTATCAACACTACTAGAGCTACCCAGCTGCCTGATCTTTGCAGCCTTCAAGTTAACATCTCTACATGTTCAGCTCTCCTTGAGCCACCAAGTCCTCCAAGTTCTCATGTTTCTCCTAGAGTAGACACCAACACCACAATTACCAATGCTACGGTTCTAGCTACTCCAGTGGGTCAACCAACGCCAAAACCTAGCATGGTGGGGTTAGGATTGGGCAGCATCAGTGCTAGTTTCAAGCCAAGGGCGGGAATTCAGCTCACAGTAGTGATTGCTATGAAGACTTTTCTGTTTCTTCTAGTTCTTCATTGA

Protein sequence

MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPPSPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVIAMKTFLFLLVLH

Homology

[Close]

BLAST of CmoCh10G002550 vs. ExPASy Swiss-Prot
Match: Q9C9B1 (Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3702 GN=LTPG10 PE=3 SV=1)

HSP 1 Score: 103.2 bits (256), Expect = 3.3e-21
Identity = 71/155 (45.81%), Postives = 90/155 (58.06%), Query Frame = 0


Query: 36  AQCTASLLPLASCAPFMQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNGT-TLS-SFPIN 95
           A C + LL LA C PF+QG A +P   CC +L Q+Y+   +CLCL LN T TLS +FPIN
Sbjct: 28  ATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQEATCLCLFLNNTSTLSPAFPIN 87

Query: 96  TTRATQLPDLCSLQVNISTCSALL--EPPS-------PPSSHVSPRVDTNTTITNATVLA 155
            T A QLP LC++  N STCS+    E PS       PPSS    ++       N+ V A
Sbjct: 88  QTLALQLPPLCNIPANSSTCSSSFPGEAPSDSSSVAPPPSSSTGSQISQGAK-NNSRVAA 147

Query: 156 TPVGQPTPKP-SMVGLGLGSISASFKPRAGIQLTV 179
           TPV Q  P+P S +GLG G  S+  K  + IQLT+
Sbjct: 148 TPVAQMAPRPTSFMGLGYGLKSSGSK--SEIQLTI 179

[Close]

BLAST of CmoCh10G002550 vs. ExPASy Swiss-Prot
Match: O64865 (Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3702 GN=LTPG14 PE=2 SV=1)

HSP 1 Score: 59.3 bits (142), Expect = 5.5e-08
Identity = 40/121 (33.06%), Postives = 62/121 (51.24%), Query Frame = 0


Query: 11  TLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPPMTCCGNLKQL 70
           T + L+ V + +A + D T       +CT  L+ +A+C P++QG A  P   CC  LKQ+
Sbjct: 11  TAIALIVVAMVVAAADDKTKDK---EECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQV 70

Query: 71  YTSVPSCLCLLL---NGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLE-PPSPPSSH 128
             S   CLC+++   N   L    IN + A  LP +C    +++ C ALL   P+ P + 
Sbjct: 71  LNSNKKCLCVIIQDRNDPDL-GLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQ 127

[Close]

BLAST of CmoCh10G002550 vs. ExPASy Swiss-Prot
Match: O64864 (Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3702 GN=LTPG13 PE=2 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 7.2e-08
Identity = 44/135 (32.59%), Postives = 68/135 (50.37%), Query Frame = 0


Query: 37  QCTASLLPLASCAPFMQGMASMPPMTCCGNLKQLYTSVPSCLCLLL---NGTTLSSFPIN 96
           +CTA L+ +A+C P++QG A  P   CC  LKQ+  S   CLC+++   N   L    +N
Sbjct: 35  ECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSDMKCLCMIIQERNDPDL-GLQVN 94

Query: 97  TTRATQLPDLCSLQVNISTCSALLE-PPSPPSSHVSPRV--DTNTTITNATVLATPVGQP 156
            + A  LP +C    +I+ C ALL   P+ P + V  ++    N T+ +A+       +P
Sbjct: 95  VSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQLAKGLNETV-SASAPTGSASEP 154

Query: 157 TPKPSMVGLGLGSIS 166
           T   S  G   G+ S
Sbjct: 155 TSMSSTPGSSAGNNS 167

[Close]

BLAST of CmoCh10G002550 vs. ExPASy Swiss-Prot
Match: Q9LJ85 (Non-specific lipid transfer protein GPI-anchored 20 OS=Arabidopsis thaliana OX=3702 GN=LTPG20 PE=2 SV=1)

HSP 1 Score: 51.2 bits (121), Expect = 1.5e-05
Identity = 45/155 (29.03%), Postives = 68/155 (43.87%), Query Frame = 0


Query: 10  LTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLAS-CAPFMQGMAS---MPPMTCCG 69
           L +  +  + LPI   Q P      ++QCT S++   S C  F+   +S    P   CC 
Sbjct: 7   LVVAMIAVLALPIRGQQQP------LSQCTPSMMTTVSPCMGFITNSSSNGTSPSSDCCN 66

Query: 70  NLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPPSPP-- 129
           +L+ L T    CLCL++ GT   + PIN T A  LP  C++      C A + P + P  
Sbjct: 67  SLRSLTTGGMGCLCLIVTGTVPFNIPINRTTAVSLPRACNMPRVPLQCQANIAPAAAPGP 126

Query: 130 ----SSHVSPRVDTNTTITNATVLATPVGQPTPKP 155
                  +SP  +T+  +   T  A      T +P
Sbjct: 127 AATFGPSMSPGPETDPIVPEPTPAAQTPQSDTTRP 155

[Close]

BLAST of CmoCh10G002550 vs. ExPASy Swiss-Prot
Match: Q1G2Y5 (Non-specific lipid transfer protein GPI-anchored 21 OS=Arabidopsis thaliana OX=3702 GN=LTPG21 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 7.4e-05
Identity = 43/143 (30.07%), Postives = 58/143 (40.56%), Query Frame = 0


Query: 38  CTASLL-PLASCAPFMQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTR 97
           C+ S+L  +  C  F+ G  S P   CCG LK L  +   CLCL++      S PIN T 
Sbjct: 33  CSPSMLSSVTGCTSFLTGGGSFPTSDCCGALKSLTGTGMDCLCLIVTAGVPISIPINRTL 92

Query: 98  ATQLPDLCSLQVNISTCSALLEP-PSPPSSHVSPR---VDTNTTITNATVLATPVGQPTP 157
           A  LP  C +      C A   P P+P  +   P     D+ T+    +    P   PT 
Sbjct: 93  AISLPRACGIPGVPVQCKASAAPLPTPGPASFGPTTSPTDSQTSDPEGSASFRPPTSPTT 152

Query: 158 KPS-----MVGLGLGSISASFKP 171
             +     + G G G     F P
Sbjct: 153 SQTPNDKDLSGSGNGGDPMGFAP 175

[Close]

BLAST of CmoCh10G002550 vs. ExPASy TrEMBL
Match: A0A6J1E230 (protein YLS3 OS=Cucurbita moschata OX=3662 GN=LOC111430130 PE=4 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 1.3e-97
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60
           MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP
Sbjct: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120

Query: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180
           SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI
Sbjct: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180

Query: 181 AMKTFLFLLVLH 193
           AMKTFLFLLVLH
Sbjct: 181 AMKTFLFLLVLH 192

[Close]

BLAST of CmoCh10G002550 vs. ExPASy TrEMBL
Match: A0A6J1JIC4 (protein YLS3 OS=Cucurbita maxima OX=3661 GN=LOC111484860 PE=4 SV=1)

HSP 1 Score: 342.0 bits (876), Expect = 1.6e-90
Identity = 180/192 (93.75%), Postives = 184/192 (95.83%), Query Frame = 0


Query: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60
           MASLPYLPCLTLL LLHV LPIAVSQDPT SSPTIAQCT SLL LASCAPF+QGMAS PP
Sbjct: 1   MASLPYLPCLTLLLLLHVTLPIAVSQDPTPSSPTIAQCTTSLLQLASCAPFVQGMASTPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNIS CSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISACSALLEPP 120

Query: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180
           SPPSSHVSPRV+TN+TITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTV I
Sbjct: 121 SPPSSHVSPRVNTNSTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAI 180

Query: 181 AMKTFLFLLVLH 193
           AMKTFLFL+VLH
Sbjct: 181 AMKTFLFLVVLH 192

[Close]

BLAST of CmoCh10G002550 vs. ExPASy TrEMBL
Match: A0A5A7TH96 (Protein YLS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00390 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 4.0e-70
Identity = 151/199 (75.88%), Postives = 166/199 (83.42%), Query Frame = 0


Query: 1   MASLPYLPC-------LTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQ 60
           MASLPYLP        L+LLFLLHV LPI +SQ PT+S+PTIAQCT S+LPLASCAPF+Q
Sbjct: 1   MASLPYLPLPQLAISYLSLLFLLHV-LPITISQGPTISTPTIAQCTTSILPLASCAPFVQ 60

Query: 61  GMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTC 120
           G+   PPM CC NLKQLY  VP+CLCLLLN T LSSFPINTTRA QLPDLCSLQVNISTC
Sbjct: 61  GVTPTPPMGCCDNLKQLYNIVPNCLCLLLNDTNLSSFPINTTRALQLPDLCSLQVNISTC 120

Query: 121 SALLEPPSPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAG 180
           SALL  PSPPSSHV PRV  N+TI+NATVLA+PV QPTPKPS+VGLGLG ISASFK R G
Sbjct: 121 SALLGSPSPPSSHVPPRVHINSTISNATVLASPVTQPTPKPSIVGLGLGKISASFKLRGG 180

Query: 181 IQLTVVIAMKTFLFLLVLH 193
           IQLT VIA+ TFLFL+VL+
Sbjct: 181 IQLTAVIAITTFLFLVVLY 198

[Close]

BLAST of CmoCh10G002550 vs. ExPASy TrEMBL
Match: A0A1S3BVJ6 (protein YLS3 OS=Cucumis melo OX=3656 GN=LOC103494119 PE=4 SV=1)

HSP 1 Score: 274.2 bits (700), Expect = 4.0e-70
Identity = 151/199 (75.88%), Postives = 166/199 (83.42%), Query Frame = 0


Query: 1   MASLPYLPC-------LTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQ 60
           MASLPYLP        L+LLFLLHV LPI +SQ PT+S+PTIAQCT S+LPLASCAPF+Q
Sbjct: 1   MASLPYLPLPQLAISYLSLLFLLHV-LPITISQGPTISTPTIAQCTTSILPLASCAPFVQ 60

Query: 61  GMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTC 120
           G+   PPM CC NLKQLY  VP+CLCLLLN T LSSFPINTTRA QLPDLCSLQVNISTC
Sbjct: 61  GVTPTPPMGCCDNLKQLYNIVPNCLCLLLNDTNLSSFPINTTRALQLPDLCSLQVNISTC 120

Query: 121 SALLEPPSPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAG 180
           SALL  PSPPSSHV PRV  N+TI+NATVLA+PV QPTPKPS+VGLGLG ISASFK R G
Sbjct: 121 SALLGSPSPPSSHVPPRVHINSTISNATVLASPVTQPTPKPSIVGLGLGKISASFKLRGG 180

Query: 181 IQLTVVIAMKTFLFLLVLH 193
           IQLT VIA+ TFLFL+VL+
Sbjct: 181 IQLTAVIAITTFLFLVVLY 198

[Close]

BLAST of CmoCh10G002550 vs. ExPASy TrEMBL
Match: A0A0A0LSN1 (AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G022510 PE=4 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.5e-66
Identity = 143/197 (72.59%), Postives = 162/197 (82.23%), Query Frame = 0


Query: 1   MASLPYLPC-------LTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQ 60
           MASLP+LP        L+LLFLLHV LPI +SQ PT+S+PTIAQCT S+LPLASCAPF+Q
Sbjct: 1   MASLPHLPLPQQAISYLSLLFLLHV-LPITISQGPTISTPTIAQCTTSILPLASCAPFVQ 60

Query: 61  GMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTC 120
           G+   PPM CC NLKQLY  VP+CLCLLLNGT LSSFPINTTRA QLPD+CSLQVNISTC
Sbjct: 61  GVTPTPPMGCCDNLKQLYNVVPNCLCLLLNGTNLSSFPINTTRALQLPDICSLQVNISTC 120

Query: 121 SALLEPPSPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAG 180
           SALL PPSPPSSHV PRV  N+TI+NATVLA+P+ QPTPKPS+VGLGLG ISASFK + G
Sbjct: 121 SALLGPPSPPSSHVPPRVHINSTISNATVLASPMTQPTPKPSIVGLGLGKISASFKLKGG 180

Query: 181 IQLTVVIAMKTFLFLLV 191
           IQLT +  M  F+ LL+
Sbjct: 181 IQLTQLAVMFFFIRLLL 196

[Close]

BLAST of CmoCh10G002550 vs. NCBI nr
Match: XP_022922067.1 (protein YLS3 [Cucurbita moschata])

HSP 1 Score: 365.5 bits (937), Expect = 2.7e-97
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0


Query: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60
           MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP
Sbjct: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120

Query: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180
           SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI
Sbjct: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180

Query: 181 AMKTFLFLLVLH 193
           AMKTFLFLLVLH
Sbjct: 181 AMKTFLFLLVLH 192

[Close]

BLAST of CmoCh10G002550 vs. NCBI nr
Match: KAG6589554.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 362.5 bits (929), Expect = 2.3e-96
Identity = 190/192 (98.96%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60
           MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPF+QGMASMPP
Sbjct: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120

Query: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180
           SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTV I
Sbjct: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAI 180

Query: 181 AMKTFLFLLVLH 193
           AMKTFLFLLVLH
Sbjct: 181 AMKTFLFLLVLH 192

[Close]

BLAST of CmoCh10G002550 vs. NCBI nr
Match: KAG7023240.1 (Protein YLS3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 362.5 bits (929), Expect = 2.3e-96
Identity = 190/192 (98.96%), Postives = 191/192 (99.48%), Query Frame = 0


Query: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60
           MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPF+QGMASMPP
Sbjct: 30  MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 89

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP
Sbjct: 90  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 149

Query: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180
           SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTV I
Sbjct: 150 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAI 209

Query: 181 AMKTFLFLLVLH 193
           AMKTFLFLLVLH
Sbjct: 210 AMKTFLFLLVLH 221

[Close]

BLAST of CmoCh10G002550 vs. NCBI nr
Match: XP_023515373.1 (protein YLS3 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 357.1 bits (915), Expect = 9.7e-95
Identity = 187/192 (97.40%), Postives = 190/192 (98.96%), Query Frame = 0


Query: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60
           MASLPYLPCLTLLFLLHV LPIAVSQDPTLSSPTIAQCTASLLPLASCAPF+QGMASMPP
Sbjct: 1   MASLPYLPCLTLLFLLHVTLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFVQGMASMPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPD+CSLQVNISTCSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDICSLQVNISTCSALLEPP 120

Query: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180
           SPPSSHVSPRV+TNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQL VVI
Sbjct: 121 SPPSSHVSPRVNTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLKVVI 180

Query: 181 AMKTFLFLLVLH 193
           AMKTFLFLLVLH
Sbjct: 181 AMKTFLFLLVLH 192

[Close]

BLAST of CmoCh10G002550 vs. NCBI nr
Match: XP_022987249.1 (protein YLS3 [Cucurbita maxima])

HSP 1 Score: 342.0 bits (876), Expect = 3.2e-90
Identity = 180/192 (93.75%), Postives = 184/192 (95.83%), Query Frame = 0


Query: 1   MASLPYLPCLTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPP 60
           MASLPYLPCLTLL LLHV LPIAVSQDPT SSPTIAQCT SLL LASCAPF+QGMAS PP
Sbjct: 1   MASLPYLPCLTLLLLLHVTLPIAVSQDPTPSSPTIAQCTTSLLQLASCAPFVQGMASTPP 60

Query: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPP 120
           MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNIS CSALLEPP
Sbjct: 61  MTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISACSALLEPP 120

Query: 121 SPPSSHVSPRVDTNTTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVVI 180
           SPPSSHVSPRV+TN+TITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTV I
Sbjct: 121 SPPSSHVSPRVNTNSTITNATVLATPVGQPTPKPSMVGLGLGSISASFKPRAGIQLTVAI 180

Query: 181 AMKTFLFLLVLH 193
           AMKTFLFL+VLH
Sbjct: 181 AMKTFLFLVVLH 192

[Close]

BLAST of CmoCh10G002550 vs. TAIR 10
Match: AT1G73890.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 103.2 bits (256), Expect = 2.4e-22
Identity = 71/155 (45.81%), Postives = 90/155 (58.06%), Query Frame = 0


Query: 36  AQCTASLLPLASCAPFMQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNGT-TLS-SFPIN 95
           A C + LL LA C PF+QG A +P   CC +L Q+Y+   +CLCL LN T TLS +FPIN
Sbjct: 28  ATCASRLLSLAPCGPFVQGFAQLPAQPCCDSLNQIYSQEATCLCLFLNNTSTLSPAFPIN 87

Query: 96  TTRATQLPDLCSLQVNISTCSALL--EPPS-------PPSSHVSPRVDTNTTITNATVLA 155
            T A QLP LC++  N STCS+    E PS       PPSS    ++       N+ V A
Sbjct: 88  QTLALQLPPLCNIPANSSTCSSSFPGEAPSDSSSVAPPPSSSTGSQISQGAK-NNSRVAA 147

Query: 156 TPVGQPTPKP-SMVGLGLGSISASFKPRAGIQLTV 179
           TPV Q  P+P S +GLG G  S+  K  + IQLT+
Sbjct: 148 TPVAQMAPRPTSFMGLGYGLKSSGSK--SEIQLTI 179

[Close]

BLAST of CmoCh10G002550 vs. TAIR 10
Match: AT2G44300.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 59.3 bits (142), Expect = 3.9e-09
Identity = 40/121 (33.06%), Postives = 62/121 (51.24%), Query Frame = 0


Query: 11  TLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLASCAPFMQGMASMPPMTCCGNLKQL 70
           T + L+ V + +A + D T       +CT  L+ +A+C P++QG A  P   CC  LKQ+
Sbjct: 11  TAIALIVVAMVVAAADDKTKDK---EECTEQLVGMATCLPYVQGQAKSPTPDCCSGLKQV 70

Query: 71  YTSVPSCLCLLL---NGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLE-PPSPPSSH 128
             S   CLC+++   N   L    IN + A  LP +C    +++ C ALL   P+ P + 
Sbjct: 71  LNSNKKCLCVIIQDRNDPDL-GLQINVSLALALPSVCHAAADVTKCPALLHLDPNSPDAQ 127

[Close]

BLAST of CmoCh10G002550 vs. TAIR 10
Match: AT2G44290.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 58.9 bits (141), Expect = 5.1e-09
Identity = 44/135 (32.59%), Postives = 68/135 (50.37%), Query Frame = 0


Query: 37  QCTASLLPLASCAPFMQGMASMPPMTCCGNLKQLYTSVPSCLCLLL---NGTTLSSFPIN 96
           +CTA L+ +A+C P++QG A  P   CC  LKQ+  S   CLC+++   N   L    +N
Sbjct: 35  ECTAQLVGMATCLPYVQGKAKSPTPDCCSGLKQVINSDMKCLCMIIQERNDPDL-GLQVN 94

Query: 97  TTRATQLPDLCSLQVNISTCSALLE-PPSPPSSHVSPRV--DTNTTITNATVLATPVGQP 156
            + A  LP +C    +I+ C ALL   P+ P + V  ++    N T+ +A+       +P
Sbjct: 95  VSLALALPSVCHATADITKCPALLHLDPNSPDAQVFYQLAKGLNETV-SASAPTGSASEP 154

Query: 157 TPKPSMVGLGLGSIS 166
           T   S  G   G+ S
Sbjct: 155 TSMSSTPGSSAGNNS 167

[Close]

BLAST of CmoCh10G002550 vs. TAIR 10
Match: AT3G22620.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 51.2 bits (121), Expect = 1.1e-06
Identity = 45/155 (29.03%), Postives = 68/155 (43.87%), Query Frame = 0


Query: 10  LTLLFLLHVLLPIAVSQDPTLSSPTIAQCTASLLPLAS-CAPFMQGMAS---MPPMTCCG 69
           L +  +  + LPI   Q P      ++QCT S++   S C  F+   +S    P   CC 
Sbjct: 7   LVVAMIAVLALPIRGQQQP------LSQCTPSMMTTVSPCMGFITNSSSNGTSPSSDCCN 66

Query: 70  NLKQLYTSVPSCLCLLLNGTTLSSFPINTTRATQLPDLCSLQVNISTCSALLEPPSPP-- 129
           +L+ L T    CLCL++ GT   + PIN T A  LP  C++      C A + P + P  
Sbjct: 67  SLRSLTTGGMGCLCLIVTGTVPFNIPINRTTAVSLPRACNMPRVPLQCQANIAPAAAPGP 126

Query: 130 ----SSHVSPRVDTNTTITNATVLATPVGQPTPKP 155
                  +SP  +T+  +   T  A      T +P
Sbjct: 127 AATFGPSMSPGPETDPIVPEPTPAAQTPQSDTTRP 155

[Close]

BLAST of CmoCh10G002550 vs. TAIR 10
Match: AT1G05450.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 5.3e-06
Identity = 43/143 (30.07%), Postives = 58/143 (40.56%), Query Frame = 0


Query: 38  CTASLL-PLASCAPFMQGMASMPPMTCCGNLKQLYTSVPSCLCLLLNGTTLSSFPINTTR 97
           C+ S+L  +  C  F+ G  S P   CCG LK L  +   CLCL++      S PIN T 
Sbjct: 33  CSPSMLSSVTGCTSFLTGGGSFPTSDCCGALKSLTGTGMDCLCLIVTAGVPISIPINRTL 92

Query: 98  ATQLPDLCSLQVNISTCSALLEP-PSPPSSHVSPR---VDTNTTITNATVLATPVGQPTP 157
           A  LP  C +      C A   P P+P  +   P     D+ T+    +    P   PT 
Sbjct: 93  AISLPRACGIPGVPVQCKASAAPLPTPGPASFGPTTSPTDSQTSDPEGSASFRPPTSPTT 152

Query: 158 KPS-----MVGLGLGSISASFKP 171
             +     + G G G     F P
Sbjct: 153 SQTPNDKDLSGSGNGGDPMGFAP 175

The following BLAST results are available for this feature:

BLAST of CmoCh10G002550 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C9B1	3.3e-21	45.81	Non-specific lipid transfer protein GPI-anchored 10 OS=Arabidopsis thaliana OX=3...	[more]
O64865	5.5e-08	33.06	Non-specific lipid transfer protein GPI-anchored 14 OS=Arabidopsis thaliana OX=3...	[more]
O64864	7.2e-08	32.59	Non-specific lipid transfer protein GPI-anchored 13 OS=Arabidopsis thaliana OX=3...	[more]
Q9LJ85	1.5e-05	29.03	Non-specific lipid transfer protein GPI-anchored 20 OS=Arabidopsis thaliana OX=3...	[more]
Q1G2Y5	7.4e-05	30.07	Non-specific lipid transfer protein GPI-anchored 21 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmoCh10G002550 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E230	1.3e-97	100.00	protein YLS3 OS=Cucurbita moschata OX=3662 GN=LOC111430130 PE=4 SV=1	[more]
A0A6J1JIC4	1.6e-90	93.75	protein YLS3 OS=Cucurbita maxima OX=3661 GN=LOC111484860 PE=4 SV=1	[more]
A0A5A7TH96	4.0e-70	75.88	Protein YLS3 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold629G00390 P...	[more]
A0A1S3BVJ6	4.0e-70	75.88	protein YLS3 OS=Cucumis melo OX=3656 GN=LOC103494119 PE=4 SV=1	[more]
A0A0A0LSN1	3.5e-66	72.59	AAI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G022510 PE=4 SV...	[more]

BLAST of CmoCh10G002550 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022922067.1	2.7e-97	100.00	protein YLS3 [Cucurbita moschata]	[more]
KAG6589554.1	2.3e-96	98.96	Protein YLS3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7023240.1	2.3e-96	98.96	Protein YLS3, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023515373.1	9.7e-95	97.40	protein YLS3 isoform X1 [Cucurbita pepo subsp. pepo]	[more]
XP_022987249.1	3.2e-90	93.75	protein YLS3 [Cucurbita maxima]	[more]

BLAST of CmoCh10G002550 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73890.1	2.4e-22	45.81	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44300.1	3.9e-09	33.06	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT2G44290.1	5.1e-09	32.59	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT3G22620.1	1.1e-06	29.03	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]
AT1G05450.2	5.3e-06	30.07	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	SMART	SM00499	aai_6	coord: 38..113 e-value: 0.002 score: 27.4
IPR016140	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain	PFAM	PF14368	LTP_2	coord: 22..113 e-value: 1.6E-14 score: 53.8
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	GENE3D	1.10.110.10		coord: 34..113 e-value: 4.9E-14 score: 53.9
IPR036312	Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamily	SUPERFAMILY	47699	Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin	coord: 37..113
IPR043325	Alpha-Amylase Inhibitors (AAI), Lipid Transfer (LT) and Seed Storage (SS) Protein	PANTHER	PTHR33044	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN-RELATED	coord: 14..155
None	No IPR available	PANTHER	PTHR33044:SF11	BIFUNCTIONAL INHIBITOR/LIPID-TRANSFER PROTEIN/SEED STORAGE 2S ALBUMIN SUPERFAMILY PROTEIN	coord: 14..155
None	No IPR available	CDD	cd00010	AAI_LTSS	coord: 45..106 e-value: 2.55115E-13 score: 60.1331

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh10G002550.1	CmoCh10G002550.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0031225	anchored component of membrane
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane