CmoCh06G002200 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh06G002200
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein
Location	Cmo_Chr06: 1188722 .. 1190846 (+)
RNA-Seq Expression	CmoCh06G002200
Synteny	CmoCh06G002200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAAAATCCACAAGAGATATTATAAGAAAATGAGCTGCCTTGGGAGTTGGCCGGAGCCGGTGGTGTGTGTGCAGTCGCTGGCGGAGAGTGGAATACGGACGATTCCAGTGAGGTACGTGAAGCCACCGTCACAGAGGCCGAGTGGCGCCGCCGTGGAGAGAAATGTTCCGAGGAATATTCCGGTGGTGGACATGGAAAAGCTGCAGAGTGCGGCGGAGGTGAAGGCGATGGCGGAAGCTTGTAAAGAGTGGGGATTTTTTCATGTAATAAACCATGGAATCAACGGTGAGATGATGGAGTGTTTGAAGGATAAGTGGACGCAGTTTTTTCATCAGCCGTTGGATTTGAAGAGACAATATGCCAATTCGCCGACCACTTATGAAGGATATGGCAGCCGGTTGGGGATTGAAAAGGCGGCGATTTTGGATTGGAGTGATTACTTTTTCATTAATTATTTGCCTGTTTCCCTCAGAAACACAGCCAAGTGGCCTGCTTTTCCTCCATCCAAGTACGTTTTTACTCATTCTTCTAGTTTTAAGACACTAAAAATGCACCGGTCGGAATGTTGGTGAGAGCTCTACCGATCGATTCGATATCGATCGGTAAATGAGCTCGTACTACCTCTTTGGTGAAACATGTTATCTATTATAAATGGTGTTTAGTTTCCTAAAGAGAAAAATGAATAATTTGTATTATTATTATTTGGGTTCGTACATCTTGTAATGTGATGTCCCACATTGATTGGAGAGTGGAAAACTTCTCCTATCAGACATGTTTTAAAGCCTTGAGGGGAATCCTGAAAGAGAAAGTCCAAAGAGGACAATATCTGCTGCTAACGGTAGATCTTGGTCATTACAAATGGTATTAGAGCCAGACACCAGATGATGTGTCAGCCTTCTGGATGTTCCCTGAAGGGGAGTAGACATGAGGCGATGTGCCAGTAAGGGCTCTGGGCCCCAAAGGGGTGGATTTGGTGGTAGTCCCACATCGATTGGAGAAAGGAATGAGTGCCAGCAAGGACGCTGGGTCCCGAAGGGGGTAGATTGTGATGTCCCACGTTGGTTAGGGAGTTGAAACTTTCCCCTAGCAGACGCATTTTAAAGCCTTGAGTGGAATCTCCAGAGGGGGAAAGCCCAAAAAGAACAATATATGCTAGCGGTAGGCTTAGGTTGTTGCATGTAATTGCATCGTTTCAATTTGACCAACTTTGTGGTCAAACAAACTCAAAATTTATCACACTTTATCTGGTCTTTTTCCAATGTTCTTCGATCCACCTTGTTATGTTTTTATAAACATTTTCTTCTAGACCAATTTAAACATAGCTCAATTGATTAAATTTTGACAGAAACAACACAAATTCAAATTTTCGTATTTTTATTGTCTGATGTGATGAATGATTAACATGCGGAAGACAAACTCGATACGTTTACGACGTTTTAGGGCTTTATTTGTGTACAGGGAATTGATAGAAGAATACGGAAAGGAGATGGTGAAGCTATGTGAGAAAGTGATGAAGGGATTATCTATGGGATTAGGGCTTGAAGAAGAGTACTTGTTAAAGGCATTTGGAGGAGAAGAAGGAATAGGAGCTTGCATGAGGGCCAATATGTATCCAAAGTGCCCTCAACCTGACCTGACTTTAGGCCTTTCGCCTCACTCCGACCCGGGCGGCATCACCATTCTTTTGCCTGACCACAACGTCCCTGGCCTCCAAGTTCGTAAAGGAGACGATTGGATCACTATCGACCCTATCCCTAATGCTTTGATTGTAAACGTTGCCGATCAAATTCAGGTTTGAATCTTTCTTTACTCAGGCTCGTAACATTGTATCTAGAAACGTTCATTATGTTACTCGTAAAAAAGAGTAATGGTTGTATGTTATTAGGTGTTGAGCAACGGGATATACAAAAGTGTGAATCATAGAGTGATCGTGAATTCAAACAAGGATCGAGTGTCATTGGCGTTCTTCTACAACCCGAGAAGTGATTTGATAATTGAACCAGCTAAAGTGCTTGTGACTGAAGAAAGGCCAGCCCTTTTTCCACCTATGACCTTTGATGAATATAGACTTCAAATTAGGAGGAGGGGTATTTGTGGAACTTCACAACAAGAACAGTCTATTTAA

mRNA sequence

TTAAAATCCACAAGAGATATTATAAGAAAATGAGCTGCCTTGGGAGTTGGCCGGAGCCGGTGGTGTGTGTGCAGTCGCTGGCGGAGAGTGGAATACGGACGATTCCAGTGAGGTACGTGAAGCCACCGTCACAGAGGCCGAGTGGCGCCGCCGTGGAGAGAAATGTTCCGAGGAATATTCCGGTGGTGGACATGGAAAAGCTGCAGAGTGCGGCGGAGGTGAAGGCGATGGCGGAAGCTTGTAAAGAGTGGGGATTTTTTCATGTAATAAACCATGGAATCAACGGTGAGATGATGGAGTGTTTGAAGGATAAGTGGACGCAGTTTTTTCATCAGCCGTTGGATTTGAAGAGACAATATGCCAATTCGCCGACCACTTATGAAGGATATGGCAGCCGGTTGGGGATTGAAAAGGCGGCGATTTTGGATTGGAGTGATTACTTTTTCATTAATTATTTGCCTGTTTCCCTCAGAAACACAGCCAAGTGGCCTGCTTTTCCTCCATCCAAGGAATTGATAGAAGAATACGGAAAGGAGATGGTGAAGCTATGTGAGAAAGTGATGAAGGGATTATCTATGGGATTAGGGCTTGAAGAAGAGTACTTGTTAAAGGCATTTGGAGGAGAAGAAGGAATAGGAGCTTGCATGAGGGCCAATATGTATCCAAAGTGCCCTCAACCTGACCTGACTTTAGGCCTTTCGCCTCACTCCGACCCGGGCGGCATCACCATTCTTTTGCCTGACCACAACGTCCCTGGCCTCCAAGTTCGTAAAGGAGACGATTGGATCACTATCGACCCTATCCCTAATGCTTTGATTGTAAACGTTGCCGATCAAATTCAGGTGTTGAGCAACGGGATATACAAAAGTGTGAATCATAGAGTGATCGTGAATTCAAACAAGGATCGAGTGTCATTGGCGTTCTTCTACAACCCGAGAAGTGATTTGATAATTGAACCAGCTAAAGTGCTTGTGACTGAAGAAAGGCCAGCCCTTTTTCCACCTATGACCTTTGATGAATATAGACTTCAAATTAGGAGGAGGGGTATTTGTGGAACTTCACAACAAGAACAGTCTATTTAA

Coding sequence (CDS)

ATGAGCTGCCTTGGGAGTTGGCCGGAGCCGGTGGTGTGTGTGCAGTCGCTGGCGGAGAGTGGAATACGGACGATTCCAGTGAGGTACGTGAAGCCACCGTCACAGAGGCCGAGTGGCGCCGCCGTGGAGAGAAATGTTCCGAGGAATATTCCGGTGGTGGACATGGAAAAGCTGCAGAGTGCGGCGGAGGTGAAGGCGATGGCGGAAGCTTGTAAAGAGTGGGGATTTTTTCATGTAATAAACCATGGAATCAACGGTGAGATGATGGAGTGTTTGAAGGATAAGTGGACGCAGTTTTTTCATCAGCCGTTGGATTTGAAGAGACAATATGCCAATTCGCCGACCACTTATGAAGGATATGGCAGCCGGTTGGGGATTGAAAAGGCGGCGATTTTGGATTGGAGTGATTACTTTTTCATTAATTATTTGCCTGTTTCCCTCAGAAACACAGCCAAGTGGCCTGCTTTTCCTCCATCCAAGGAATTGATAGAAGAATACGGAAAGGAGATGGTGAAGCTATGTGAGAAAGTGATGAAGGGATTATCTATGGGATTAGGGCTTGAAGAAGAGTACTTGTTAAAGGCATTTGGAGGAGAAGAAGGAATAGGAGCTTGCATGAGGGCCAATATGTATCCAAAGTGCCCTCAACCTGACCTGACTTTAGGCCTTTCGCCTCACTCCGACCCGGGCGGCATCACCATTCTTTTGCCTGACCACAACGTCCCTGGCCTCCAAGTTCGTAAAGGAGACGATTGGATCACTATCGACCCTATCCCTAATGCTTTGATTGTAAACGTTGCCGATCAAATTCAGGTGTTGAGCAACGGGATATACAAAAGTGTGAATCATAGAGTGATCGTGAATTCAAACAAGGATCGAGTGTCATTGGCGTTCTTCTACAACCCGAGAAGTGATTTGATAATTGAACCAGCTAAAGTGCTTGTGACTGAAGAAAGGCCAGCCCTTTTTCCACCTATGACCTTTGATGAATATAGACTTCAAATTAGGAGGAGGGGTATTTGTGGAACTTCACAACAAGAACAGTCTATTTAA

Protein sequence

MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI

Homology

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BLAST of CmoCh06G002200 vs. ExPASy Swiss-Prot
Match: O80449 (Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 3.3e-120
Identity = 201/341 (58.94%), Postives = 258/341 (75.66%), Query Frame = 0


Query: 7   WPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAE-VK 66
           WPEP+V VQSL+++G+ T+P RYVKP  QRP     + +    IPV+DM  +    E ++
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLR 64

Query: 67  AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRLG 126
            +  AC+EWGFF ++NHG+   +ME ++  W +FF  PL+ KR+YANSP TYEGYGSRLG
Sbjct: 65  LVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLG 124

Query: 127 IEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKGLSMG 186
           + K A LDWSDYFF+NYLP S+RN +KWP+ PP  +ELIE+YG+E+ KLCE++ + LS  
Sbjct: 125 VVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSES 184

Query: 187 LGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 246
           LGL+   L++A GG + +GA +R N YPKCPQP LTLGLS HSDPGGITILLPD  V GL
Sbjct: 185 LGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGL 244

Query: 247 QVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPRS 306
           QVR+GD W+TI  +PNALIVN+ DQ+Q+LSNGIYKSV H+VIVNS  +RVSLAFFYNPRS
Sbjct: 245 QVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRS 304

Query: 307 DLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQ 346
           D+ + P + LVT  RPAL+ P+ FDEYR  IR++G CG +Q
Sbjct: 305 DIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQ 345

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BLAST of CmoCh06G002200 vs. ExPASy Swiss-Prot
Match: Q9SRM3 (Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.7e-119
Identity = 202/350 (57.71%), Postives = 264/350 (75.43%), Query Frame = 0


Query: 7   WPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERN---VPRNIPVVDMEKLQSAAE 66
           WPEP+V VQSLAES + ++P RY+KPPSQRP    ++        NIP++D++ L S  E
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 67  --VKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYG 126
              K ++EAC+EWGFF VINHG+  E+M+  ++ W  FF+ P++ K  Y+NSP TYEGYG
Sbjct: 109 DDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYG 168

Query: 127 SRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKG 186
           SRLG+EK AILDW+DY+++++LP++L++  KWP+ P + +E+ +EYGKE+VKL  ++M  
Sbjct: 169 SRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTI 228

Query: 187 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 246
           LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD  
Sbjct: 229 LSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQ 288

Query: 247 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 306
           V GLQVR GD WIT++P+ +A IVN+ DQIQ+LSN  YKSV HRVIVNS K+RVSLAFFY
Sbjct: 289 VVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFY 348

Query: 307 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 351
           NP+SD+ I+P + LVT   P L+PPMTFD+YRL IR +G  G S  E  I
Sbjct: 349 NPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKSHVESHI 397

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BLAST of CmoCh06G002200 vs. ExPASy Swiss-Prot
Match: Q9FFF6 (Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 2.0e-117
Identity = 200/354 (56.50%), Postives = 262/354 (74.01%), Query Frame = 0


Query: 4   LGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAE 63
           +  WPEP+V VQSLAES + ++P RY+KP S RP+    +     NIP++D+E L S   
Sbjct: 17  MDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPT-TTEDAPTATNIPIIDLEGLFSEEG 76

Query: 64  VK------AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTY 123
           +        ++EAC+ WGFF V+NHG+  E+M+  ++ W +FFH P++ K  Y+NSP TY
Sbjct: 77  LSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTY 136

Query: 124 EGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEK 183
           EGYGSRLG+EK A LDWSDY+F++ LP  L++  KWP+FPP+ +E+I+EYG+E+VKL  +
Sbjct: 137 EGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGR 196

Query: 184 VMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILL 243
           +M+ LS  LGL+E+   +AFGG E IGAC+R N YPKCP+P+L LGLSPHSDPGG+TILL
Sbjct: 197 IMRVLSTNLGLKEDKFQEAFGG-ENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILL 256

Query: 244 PDHNVPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSL 303
           PD  V GLQVRK D WIT+ P P+A IVN+ DQIQ+LSN  YKSV HRVIVNS+K+RVSL
Sbjct: 257 PDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSL 316

Query: 304 AFFYNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 351
           AFFYNP+SD+ I+P + LV+   P L+PPMTFD+YRL IR +G  G S  E  I
Sbjct: 317 AFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKSHVESHI 368

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BLAST of CmoCh06G002200 vs. ExPASy Swiss-Prot
Match: Q9LY48 (Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 4.8e-111
Identity = 193/349 (55.30%), Postives = 256/349 (73.35%), Query Frame = 0


Query: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPR--NIPVVDMEKL 60
           M+    WPEP+V VQSL+ES +  IP RYVKP SQRP+     ++ P+   IP++D+ +L
Sbjct: 1   MNIFQDWPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRL 60

Query: 61  QS------AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYAN 120
            +      A  +  +++AC+E GFF V+NHG++ ++M+  K  W +FF+ P++LK  +AN
Sbjct: 61  YTDDLTLQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHAN 120

Query: 121 SPTTYEGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFP-PSKELIEEYGKEMV 180
           SP TYEGYGSRLG+EK AILDWSDY++++Y P SL++  KWP+ P   +E++E+Y KEMV
Sbjct: 121 SPKTYEGYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMV 180

Query: 181 KLCEKVMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG 240
           KLCE +MK LS  LGL+E+ L  AFGG+E  G C+R N YPKCPQP+LTLG+SPHSDPGG
Sbjct: 181 KLCENLMKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGG 240

Query: 241 ITILLPDHNVPGLQVRKGDD-WITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSN 300
           +TILLPD  V  LQVR  DD WIT++P P+A IVN+ DQIQ+LSN IYKSV HRVIVN  
Sbjct: 241 LTILLPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPE 300

Query: 301 KDRVSLAFFYNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRG 340
            +R+SLAFFYNP+ ++ IEP K LVT + PAL+   T+D YR  IR +G
Sbjct: 301 NERLSLAFFYNPKGNVPIEPLKELVTVDSPALYSSTTYDRYRQFIRTQG 349

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BLAST of CmoCh06G002200 vs. ExPASy Swiss-Prot
Match: P51091 (Leucoanthocyanidin dioxygenase OS=Malus domestica OX=3750 GN=ANS PE=2 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 1.9e-62
Identity = 128/333 (38.44%), Postives = 200/333 (60.06%), Query Frame = 0


Query: 14  VQSLAESGIRTIPVRYVKPPSQRPSGAAV----ERNVPRNIPVVDMEKLQSAAE-VKA-- 73
           V++LA SGI TIP  Y++P  +  +   +    + N    +P +D+++++S  E V+A  
Sbjct: 11  VETLAGSGISTIPKEYIRPKDELVNIGDIFEQEKNNEGPQVPTIDLKEIESDNEKVRAKC 70

Query: 74  ---MAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTT--YEGYG 133
              + +A  +WG  H++NHGI+ E+M+ ++     FF  P++ K +YAN   +   +GYG
Sbjct: 71  REKLKKAAVDWGVMHLVNHGISDELMDKVRKAGKAFFDLPIEQKEKYANDQASGKIQGYG 130

Query: 134 SRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSK-ELIEEYGKEMVKLCEKVMKG 193
           S+L    +  L+W DYFF    P   R+ + WP  P    E   EY K++ +L  KV+K 
Sbjct: 131 SKLANNASGQLEWEDYFFHCVYPEDKRDLSIWPQTPADYIEATAEYAKQLRELATKVLKV 190

Query: 194 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 253
           LS+GLGL+E  L K  GG E +   M+ N YPKCPQP+L LG+  H+D   +T +L  HN
Sbjct: 191 LSLGLGLDEGRLEKEVGGLEELLLQMKINYYPKCPQPELALGVEAHTDVSALTFIL--HN 250

Query: 254 -VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFF 313
            VPGLQ+     W+T   +PN++++++ D +++LSNG YKS+ HR +VN  K R+S A F
Sbjct: 251 MVPGLQLFYEGKWVTAKCVPNSIVMHIGDTLEILSNGKYKSILHRGMVNKEKVRISWAVF 310

Query: 314 YN-PRSDLIIEPAKVLVTEERPALFPPMTFDEY 332
              P+  +I++P    V+E+ PA+FPP TF E+
Sbjct: 311 CEPPKEKIILKPLPETVSEDEPAMFPPRTFAEH 341

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BLAST of CmoCh06G002200 vs. ExPASy TrEMBL
Match: A0A6J1G9Z8 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita moschata OX=3662 GN=LOC111452304 PE=3 SV=1)

HSP 1 Score: 727.6 bits (1877), Expect = 2.4e-206
Identity = 350/350 (100.00%), Postives = 350/350 (100.00%), Query Frame = 0


Query: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60
           MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60

Query: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120
           AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY
Sbjct: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180
           GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG
Sbjct: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180

Query: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240
           LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240

Query: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300
           VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY
Sbjct: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300

Query: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 351
           NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI
Sbjct: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 350

[Close]

BLAST of CmoCh06G002200 vs. ExPASy TrEMBL
Match: A0A6J1I649 (probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN=LOC111470253 PE=3 SV=1)

HSP 1 Score: 714.5 bits (1843), Expect = 2.1e-202
Identity = 344/350 (98.29%), Postives = 346/350 (98.86%), Query Frame = 0


Query: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQS 60
           MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAV+RNV RNIPVVDMEKLQS
Sbjct: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVKRNVWRNIPVVDMEKLQS 60

Query: 61  AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120
           AAEV AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY
Sbjct: 61  AAEVTAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGY 120

Query: 121 GSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180
           GSRLGIEK AILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG
Sbjct: 121 GSRLGIEKGAILDWSDYFFINYLPVSLRNTAKWPAFPPSKELIEEYGKEMVKLCEKVMKG 180

Query: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240
           LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN
Sbjct: 181 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 240

Query: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300
           VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY
Sbjct: 241 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 300

Query: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 351
           NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIR+RGICGT QQEQSI
Sbjct: 301 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRKRGICGTLQQEQSI 350

[Close]

BLAST of CmoCh06G002200 vs. ExPASy TrEMBL
Match: A0A6J1J756 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3661 GN=LOC111481976 PE=3 SV=1)

HSP 1 Score: 616.7 bits (1589), Expect = 6.0e-173
Identity = 294/347 (84.73%), Postives = 318/347 (91.64%), Query Frame = 0


Query: 6   SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVER-NVPRNIPVVDMEKLQSAAEV 65
           SWPEPVVCVQSLAESGI+TIPVRYVKPP QRP GAA+E   V RNIP+VDM KL+SAA V
Sbjct: 2   SWPEPVVCVQSLAESGIKTIPVRYVKPPCQRPGGAAMESVEVQRNIPMVDMAKLESAAAV 61

Query: 66  KAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRL 125
           + MAEAC+EWGFFHVINHGI+GE+MEC+K+ W QFF QPLDLK+QYANSP TYEGYGSRL
Sbjct: 62  RDMAEACQEWGFFHVINHGISGELMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 121

Query: 126 GIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPP-SKELIEEYGKEMVKLCEKVMKGLSM 185
           GIEK AILDWSDYFF+NY+P SLRN A WPAFPP SK+LIEEYG E+VKLC K+MK LS+
Sbjct: 122 GIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSSKKLIEEYGSEVVKLCGKLMKRLSV 181

Query: 186 GLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 245
           GLGL+EEYLL AFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVPG
Sbjct: 182 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVPG 241

Query: 246 LQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPR 305
           LQVRKGD+WITIDPIPNALIVN+ DQIQVLSN IYKSV HRVIVNSNKDRVSLAFFYNP+
Sbjct: 242 LQVRKGDNWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 301

Query: 306 SDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 351
           SDLIIEPAK LVTEERPALFP MTFDEYRL IR+RGICGTSQ EQS+
Sbjct: 302 SDLIIEPAKELVTEERPALFPRMTFDEYRLHIRKRGICGTSQHEQSL 348

[Close]

BLAST of CmoCh06G002200 vs. ExPASy TrEMBL
Match: A0A6J1F503 (probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX=3662 GN=LOC111442217 PE=3 SV=1)

HSP 1 Score: 614.4 bits (1583), Expect = 3.0e-172
Identity = 294/347 (84.73%), Postives = 316/347 (91.07%), Query Frame = 0


Query: 6   SWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVER-NVPRNIPVVDMEKLQSAAEV 65
           SWPEPVVCVQSLAESGIRTIPVRYVKPP QRP GAA+E   V  NIPVVDM KL+SAA V
Sbjct: 2   SWPEPVVCVQSLAESGIRTIPVRYVKPPCQRPGGAAMESVEVQMNIPVVDMAKLESAAAV 61

Query: 66  KAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRL 125
           + MAEAC+EWGFFHVINHGI+GE+MEC+K+ W QFF QPLDLK+QYANSP TYEGYGSRL
Sbjct: 62  RDMAEACREWGFFHVINHGISGEVMECVKESWKQFFDQPLDLKKQYANSPMTYEGYGSRL 121

Query: 126 GIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKGLSM 185
           GIEK AILDWSDYFF+NY+P SLRN A WPAFPPS K+LIEEYG E+VKLC K+MK LS+
Sbjct: 122 GIEKGAILDWSDYFFLNYMPTSLRNPAMWPAFPPSTKKLIEEYGNEVVKLCGKLMKRLSV 181

Query: 186 GLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPG 245
           GLGL+EEYLL AFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG+TILLPDHNVPG
Sbjct: 182 GLGLKEEYLLNAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGMTILLPDHNVPG 241

Query: 246 LQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPR 305
           LQVRKGD WITIDPIPNALIVN+ DQIQVLSN IYKSV HRVIVNSNKDRVSLAFFYNP+
Sbjct: 242 LQVRKGDHWITIDPIPNALIVNIGDQIQVLSNAIYKSVKHRVIVNSNKDRVSLAFFYNPK 301

Query: 306 SDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 351
           SDLIIEPAK LV+EERPALFP MTFDEYRL IR+RGICGTSQ EQS+
Sbjct: 302 SDLIIEPAKELVSEERPALFPRMTFDEYRLDIRKRGICGTSQHEQSL 348

[Close]

BLAST of CmoCh06G002200 vs. ExPASy TrEMBL
Match: A0A0A0LCS8 (Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G731160 PE=3 SV=1)

HSP 1 Score: 584.3 bits (1505), Expect = 3.3e-163
Identity = 274/348 (78.74%), Postives = 310/348 (89.08%), Query Frame = 0


Query: 2   SCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSA 61
           SCL SWPEPVVCVQSLAESGI  IP RYVKPPSQRP G  V++   RNIPVVDMEK++S 
Sbjct: 4   SCLQSWPEPVVCVQSLAESGISKIPGRYVKPPSQRPDGGTVKK--MRNIPVVDMEKVESG 63

Query: 62  AEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYG 121
           A VK MAEAC+EWGFF +INHGI+GEM+EC+K+ W +FF+QPLDLK QYAN+P TYEGYG
Sbjct: 64  AAVKEMAEACREWGFFQIINHGISGEMIECVKESWKEFFNQPLDLKNQYANTPATYEGYG 123

Query: 122 SRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKG 181
           SRLGIEK AILDWSDYFF+N+LP+SLRN  KWPAFP S K++IEEYG E+VK+C K+MKG
Sbjct: 124 SRLGIEKGAILDWSDYFFLNFLPLSLRNPTKWPAFPSSFKKVIEEYGDEVVKVCAKLMKG 183

Query: 182 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 241
           LSMGLGLEEEYLL AFGGE+GIG  MRANMYPKCPQPDLTLGLS HSDPGGITILL DHN
Sbjct: 184 LSMGLGLEEEYLLNAFGGEKGIGGSMRANMYPKCPQPDLTLGLSSHSDPGGITILLADHN 243

Query: 242 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 301
           VPGLQV KG+DWIT+DPIPNALIVN+ DQIQVLSNGIYKSV HRV+VN NK+RVSLAFFY
Sbjct: 244 VPGLQVLKGNDWITVDPIPNALIVNIGDQIQVLSNGIYKSVKHRVMVNPNKERVSLAFFY 303

Query: 302 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQ 349
           NP+ DLIIEP+K L+T+ERP+LFPPMTFDEYRL IR++GICGTS+ +Q
Sbjct: 304 NPKGDLIIEPSKELLTKERPSLFPPMTFDEYRLYIRKKGICGTSKHQQ 349

[Close]

BLAST of CmoCh06G002200 vs. TAIR 10
Match: AT2G38240.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 433.0 bits (1112), Expect = 2.4e-121
Identity = 201/341 (58.94%), Postives = 258/341 (75.66%), Query Frame = 0


Query: 7   WPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAE-VK 66
           WPEP+V VQSL+++G+ T+P RYVKP  QRP     + +    IPV+DM  +    E ++
Sbjct: 5   WPEPIVSVQSLSQTGVPTVPNRYVKPAHQRPVFNTTQSDAGIEIPVLDMNDVWGKPEGLR 64

Query: 67  AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYGSRLG 126
            +  AC+EWGFF ++NHG+   +ME ++  W +FF  PL+ KR+YANSP TYEGYGSRLG
Sbjct: 65  LVRSACEEWGFFQMVNHGVTHSLMERVRGAWREFFELPLEEKRKYANSPDTYEGYGSRLG 124

Query: 127 IEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKGLSMG 186
           + K A LDWSDYFF+NYLP S+RN +KWP+ PP  +ELIE+YG+E+ KLCE++ + LS  
Sbjct: 125 VVKDAKLDWSDYFFLNYLPSSIRNPSKWPSQPPKIRELIEKYGEEVRKLCERLTETLSES 184

Query: 187 LGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHNVPGL 246
           LGL+   L++A GG + +GA +R N YPKCPQP LTLGLS HSDPGGITILLPD  V GL
Sbjct: 185 LGLKPNKLMQALGGGDKVGASLRTNFYPKCPQPQLTLGLSSHSDPGGITILLPDEKVAGL 244

Query: 247 QVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFYNPRS 306
           QVR+GD W+TI  +PNALIVN+ DQ+Q+LSNGIYKSV H+VIVNS  +RVSLAFFYNPRS
Sbjct: 245 QVRRGDGWVTIKSVPNALIVNIGDQLQILSNGIYKSVEHQVIVNSGMERVSLAFFYNPRS 304

Query: 307 DLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQ 346
           D+ + P + LVT  RPAL+ P+ FDEYR  IR++G CG +Q
Sbjct: 305 DIPVGPIEELVTANRPALYKPIRFDEYRSLIRQKGPCGKNQ 345

[Close]

BLAST of CmoCh06G002200 vs. TAIR 10
Match: AT3G11180.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 429.5 bits (1103), Expect = 2.6e-120
Identity = 202/350 (57.71%), Postives = 264/350 (75.43%), Query Frame = 0


Query: 7   WPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERN---VPRNIPVVDMEKLQSAAE 66
           WPEP+V VQSLAES + ++P RY+KPPSQRP    ++        NIP++D++ L S  E
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 67  --VKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYG 126
              K ++EAC+EWGFF VINHG+  E+M+  ++ W  FF+ P++ K  Y+NSP TYEGYG
Sbjct: 109 DDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYG 168

Query: 127 SRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKG 186
           SRLG+EK AILDW+DY+++++LP++L++  KWP+ P + +E+ +EYGKE+VKL  ++M  
Sbjct: 169 SRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTI 228

Query: 187 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 246
           LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD  
Sbjct: 229 LSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQ 288

Query: 247 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 306
           V GLQVR GD WIT++P+ +A IVN+ DQIQ+LSN  YKSV HRVIVNS K+RVSLAFFY
Sbjct: 289 VVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFY 348

Query: 307 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 351
           NP+SD+ I+P + LVT   P L+PPMTFD+YRL IR +G  G S  E  I
Sbjct: 349 NPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQGPRGKSHVESHI 397

[Close]

BLAST of CmoCh06G002200 vs. TAIR 10
Match: AT3G11180.2 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 425.6 bits (1093), Expect = 3.8e-119
Identity = 198/339 (58.41%), Postives = 260/339 (76.70%), Query Frame = 0


Query: 7   WPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERN---VPRNIPVVDMEKLQSAAE 66
           WPEP+V VQSLAES + ++P RY+KPPSQRP    ++        NIP++D++ L S  E
Sbjct: 49  WPEPIVRVQSLAESNLTSLPDRYIKPPSQRPQTTIIDHQPEVADINIPIIDLDSLFSGNE 108

Query: 67  --VKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTYEGYG 126
              K ++EAC+EWGFF VINHG+  E+M+  ++ W  FF+ P++ K  Y+NSP TYEGYG
Sbjct: 109 DDKKRISEACREWGFFQVINHGVKPELMDAARETWKSFFNLPVEAKEVYSNSPRTYEGYG 168

Query: 127 SRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEKVMKG 186
           SRLG+EK AILDW+DY+++++LP++L++  KWP+ P + +E+ +EYGKE+VKL  ++M  
Sbjct: 169 SRLGVEKGAILDWNDYYYLHFLPLALKDFNKWPSLPSNIREMNDEYGKELVKLGGRLMTI 228

Query: 187 LSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILLPDHN 246
           LS  LGL  E L +AFGGE+ +GAC+R N YPKCPQP+L LGLSPHSDPGG+TILLPD  
Sbjct: 229 LSSNLGLRAEQLQEAFGGED-VGACLRVNYYPKCPQPELALGLSPHSDPGGMTILLPDDQ 288

Query: 247 VPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSLAFFY 306
           V GLQVR GD WIT++P+ +A IVN+ DQIQ+LSN  YKSV HRVIVNS K+RVSLAFFY
Sbjct: 289 VVGLQVRHGDTWITVNPLRHAFIVNIGDQIQILSNSKYKSVEHRVIVNSEKERVSLAFFY 348

Query: 307 NPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRG 340
           NP+SD+ I+P + LVT   P L+PPMTFD+YRL IR +G
Sbjct: 349 NPKSDIPIQPMQQLVTSTMPPLYPPMTFDQYRLFIRTQG 386

[Close]

BLAST of CmoCh06G002200 vs. TAIR 10
Match: AT5G05600.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 423.7 bits (1088), Expect = 1.4e-118
Identity = 200/354 (56.50%), Postives = 262/354 (74.01%), Query Frame = 0


Query: 4   LGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPRNIPVVDMEKLQSAAE 63
           +  WPEP+V VQSLAES + ++P RY+KP S RP+    +     NIP++D+E L S   
Sbjct: 17  MDEWPEPIVRVQSLAESNLSSLPDRYIKPASLRPT-TTEDAPTATNIPIIDLEGLFSEEG 76

Query: 64  VK------AMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYANSPTTY 123
           +        ++EAC+ WGFF V+NHG+  E+M+  ++ W +FFH P++ K  Y+NSP TY
Sbjct: 77  LSDDVIMARISEACRGWGFFQVVNHGVKPELMDAARENWREFFHMPVNAKETYSNSPRTY 136

Query: 124 EGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFPPS-KELIEEYGKEMVKLCEK 183
           EGYGSRLG+EK A LDWSDY+F++ LP  L++  KWP+FPP+ +E+I+EYG+E+VKL  +
Sbjct: 137 EGYGSRLGVEKGASLDWSDYYFLHLLPHHLKDFNKWPSFPPTIREVIDEYGEELVKLSGR 196

Query: 184 VMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGGITILL 243
           +M+ LS  LGL+E+   +AFGG E IGAC+R N YPKCP+P+L LGLSPHSDPGG+TILL
Sbjct: 197 IMRVLSTNLGLKEDKFQEAFGG-ENIGACLRVNYYPKCPRPELALGLSPHSDPGGMTILL 256

Query: 244 PDHNVPGLQVRKGDDWITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSNKDRVSL 303
           PD  V GLQVRK D WIT+ P P+A IVN+ DQIQ+LSN  YKSV HRVIVNS+K+RVSL
Sbjct: 257 PDDQVFGLQVRKDDTWITVKPHPHAFIVNIGDQIQILSNSTYKSVEHRVIVNSDKERVSL 316

Query: 304 AFFYNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRGICGTSQQEQSI 351
           AFFYNP+SD+ I+P + LV+   P L+PPMTFD+YRL IR +G  G S  E  I
Sbjct: 317 AFFYNPKSDIPIQPLQELVSTHNPPLYPPMTFDQYRLFIRTQGPQGKSHVESHI 368

[Close]

BLAST of CmoCh06G002200 vs. TAIR 10
Match: AT3G55970.1 (jasmonate-regulated gene 21 )

HSP 1 Score: 402.5 bits (1033), Expect = 3.4e-112
Identity = 193/349 (55.30%), Postives = 256/349 (73.35%), Query Frame = 0


Query: 1   MSCLGSWPEPVVCVQSLAESGIRTIPVRYVKPPSQRPSGAAVERNVPR--NIPVVDMEKL 60
           M+    WPEP+V VQSL+ES +  IP RYVKP SQRP+     ++ P+   IP++D+ +L
Sbjct: 1   MNIFQDWPEPIVRVQSLSESNLGAIPNRYVKPLSQRPNITPHNKHNPQTTTIPIIDLGRL 60

Query: 61  QS------AAEVKAMAEACKEWGFFHVINHGINGEMMECLKDKWTQFFHQPLDLKRQYAN 120
            +      A  +  +++AC+E GFF V+NHG++ ++M+  K  W +FF+ P++LK  +AN
Sbjct: 61  YTDDLTLQAKTLDEISKACRELGFFQVVNHGMSPQLMDQAKATWREFFNLPMELKNMHAN 120

Query: 121 SPTTYEGYGSRLGIEKAAILDWSDYFFINYLPVSLRNTAKWPAFP-PSKELIEEYGKEMV 180
           SP TYEGYGSRLG+EK AILDWSDY++++Y P SL++  KWP+ P   +E++E+Y KEMV
Sbjct: 121 SPKTYEGYGSRLGVEKGAILDWSDYYYLHYQPSSLKDYTKWPSLPLHCREILEDYCKEMV 180

Query: 181 KLCEKVMKGLSMGLGLEEEYLLKAFGGEEGIGACMRANMYPKCPQPDLTLGLSPHSDPGG 240
           KLCE +MK LS  LGL+E+ L  AFGG+E  G C+R N YPKCPQP+LTLG+SPHSDPGG
Sbjct: 181 KLCENLMKILSKNLGLQEDRLQNAFGGKEESGGCLRVNYYPKCPQPELTLGISPHSDPGG 240

Query: 241 ITILLPDHNVPGLQVRKGDD-WITIDPIPNALIVNVADQIQVLSNGIYKSVNHRVIVNSN 300
           +TILLPD  V  LQVR  DD WIT++P P+A IVN+ DQIQ+LSN IYKSV HRVIVN  
Sbjct: 241 LTILLPDEQVASLQVRGSDDAWITVEPAPHAFIVNMGDQIQMLSNSIYKSVEHRVIVNPE 300

Query: 301 KDRVSLAFFYNPRSDLIIEPAKVLVTEERPALFPPMTFDEYRLQIRRRG 340
            +R+SLAFFYNP+ ++ IEP K LVT + PAL+   T+D YR  IR +G
Sbjct: 301 NERLSLAFFYNPKGNVPIEPLKELVTVDSPALYSSTTYDRYRQFIRTQG 349

The following BLAST results are available for this feature:

BLAST of CmoCh06G002200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O80449	3.3e-120	58.94	Jasmonate-induced oxygenase 4 OS=Arabidopsis thaliana OX=3702 GN=JOX4 PE=1 SV=1	[more]
Q9SRM3	3.7e-119	57.71	Jasmonate-induced oxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=JOX1 PE=1 SV=1	[more]
Q9FFF6	2.0e-117	56.50	Jasmonate-induced oxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=JOX2 PE=1 SV=1	[more]
Q9LY48	4.8e-111	55.30	Jasmonate-induced oxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=JOX3 PE=1 SV=1	[more]
P51091	1.9e-62	38.44	Leucoanthocyanidin dioxygenase OS=Malus domestica OX=3750 GN=ANS PE=2 SV=1	[more]

BLAST of CmoCh06G002200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G9Z8	2.4e-206	100.00	probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita moschata OX=3662 ...	[more]
A0A6J1I649	2.1e-202	98.29	probable 2-oxoglutarate-dependent dioxygenase ANS OS=Cucurbita maxima OX=3661 GN...	[more]
A0A6J1J756	6.0e-173	84.73	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita maxima OX=3...	[more]
A0A6J1F503	3.0e-172	84.73	probable 2-oxoglutarate-dependent dioxygenase At5g05600 OS=Cucurbita moschata OX...	[more]
A0A0A0LCS8	3.3e-163	78.74	Fe2OG dioxygenase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G...	[more]

BLAST of CmoCh06G002200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G38240.1	2.4e-121	58.94	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G11180.1	2.6e-120	57.71	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G11180.2	3.8e-119	58.41	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT5G05600.1	1.4e-118	56.50	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein	[more]
AT3G55970.1	3.4e-112	55.30	jasmonate-regulated gene 21	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PRINTS	PR00682	IPNSYNTHASE	coord: 67..84 score: 43.43 coord: 290..308 score: 38.76
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 8..347
None	No IPR available	PANTHER	PTHR47990:SF62	LEUCOANTHOCYANIDIN DIOXYGENASE-LIKE PROTEIN	coord: 8..347
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 11..336
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 9..338 e-value: 7.8E-105 score: 352.9
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 50..154 e-value: 9.5E-24 score: 84.4
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 204..302 e-value: 5.2E-27 score: 94.3
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 201..302 score: 13.860356

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh06G002200.1	CmoCh06G002200.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:1900366	negative regulation of defense response to insect
biological_process	GO:1900150	regulation of defense response to fungus
biological_process	GO:2000022	regulation of jasmonic acid mediated signaling pathway
cellular_component	GO:0005829	cytosol
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0120091	jasmonic acid hydrolase
molecular_function	GO:0046872	metal ion binding