CmoCh04G024620 (gene) Cucurbita moschata (Rifu) v1

Overview


Name	CmoCh04G024620
Type	gene
Organism	Cucurbita moschata (Cucurbita moschata (Rifu) v1)
Description	aminoacylase-1
Location	Cmo_Chr04: 18205007 .. 18208061 (-)
RNA-Seq Expression	CmoCh04G024620
Synteny	CmoCh04G024620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAATAATTATGTACCGGAGCAGCACCGGTCTTCTGTCGCCGGCGAGTTGAAGAGGCAAAAAGCTAAGTTTTGACAATCCATTCATCGGCGGTTGTCTTCACCATTCGGCAGCTCTCCGGCTTACTCAACTTCAGAAGTTCCGACTTGCTCAACTGCTTGACCGCGGCACCAACGGAGGCGCCCATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTCATTTCCTCTCTCTTTCAGTCATTCGCAGTACGATGATGACGAATCCGATCAAAACTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCTGTCTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTCGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGACTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCGTCCGGACCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCCATTCGGAGCCTCCGAAGTCGGAATTTCATCCCTGACCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGAAAATTCGGGCTGAAGGAACCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGTGAGTAGTCCTTGCGAAATGAGTTGCAGGGTTTAAGCCCATCTGCCTATGTCTCTTTTCGCCATTAATATTGTGATTTGTCTCAGGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCGACCGCCGACCCAGATGCCATTAGAAGAAGAATTAGGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAGGTAAGTGGCTAGCTGCCTGATTTGATTCCCAACACCATCTGGTTCCTGTTTTATTAGCTCTCATCATTAGTATTCTGTTCTTGTTCATGGCTTGCTTATGGTTCTGCAATCTAAAGAAAAAAGCTTGGAGAAGTCCTCATATGACTTGTCATTTTATCTTGCTGAAAACAGAGTAAATCTGTAAGATTATGATCTTAAACCAATTTTGATGTGGAAAGAATTTGAATTCTGATGGAGGATGTCTTTGTTTGTTCTATATGTTATATTATGAAACTCGTCTACTCCATCCACAACAAAATGCACCGGGCAATGGCATGCGAATTTTCCCATGTCATGGGAGGAATTGATTAGATTTTCTCATTTCTTGTGAGATCTAATATCGATTGGAGAGGGGAACGAAACATTCTTTATAAGGGTGTGGAAATCTCTCTCTAGCCGATACGTTTTGAAAACCTCGAGGAGAAACTCGAAAGGTAAAGCCCAAAGAGGACAATATTTGTTAACGGTGGCTTTGGGCCGTTACAAATGGTATTAGAGCCTGACATTAGGCGATGTGCCAATGCAGAGGCTGAGTCCCGAAAGGGGTGGACACGAGGCAGTGTGCCAGCAAGGATGTTGGGCCTCGAAGGGGGTGGACTGTAAGATCCCACATCAGCTGGAGGGGGAACGAAGCATTCTTTATAAGGGTGTGAAAACCTCTCCCTAACAGACGCGTTTTCGTCTTAAAATCTTGAGAGGAAACCGAAAGGGAAAGCCCAAAGAGGACAAGGATTTGGGCCACTTCTGCTCTACGTACCCAAGATTGAATGCTTGTTTTGTGCATGTCTAGCCTTTAGCAATTAGATATTCAAGTATTTGCATCATATAGCAGTCTCTTACGAATTACACACTATCTATTTGTACGTTATCTTAGCAATGTCTGATAGCTTAAACATAATGGATCTGATACCTGCAGATTATTGAGAAAGGACCCATAAGAGACTATTTGGGACGTCCATTATTGACGATGACTAACGATTCGAATCCGTGGTGGTCTGTTTTCAAGACAGCCATCTCAGACGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTAGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCACGAACACAACGAGGTTATACTAGACTCCATTGTGTCTCTCTTTTTTATCCATGTTCTTGCTCGATTTGCGTTATGTTCGTATTAATCGGTCATCTCCTTTCCTCGTACCTTGTTGCAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAGATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCAGTAAGTGCTCCCATGGATGAACACTGCTATTGTAAGTACTGAAGTTCTCTTTTCCACACAACGTCACAATTCACTTGTAAACATTCTCTTTCGTTTTATTACCTTTTCATGAGATTCGAATAGGGTAAATAGTAAACAAAAAATGATCTTTTGTAGTATATTGAGATTGTCTCCTCTAAGCGCATCCTTCCTAAATTAAATGGGTATCAAATAACGAGATATATTCTATAGGTTGTATAAATGCGTTCTTGAATTCTAAGAAGTTATTTAGCTATCGACAATGAAATGTATATTACTCGAAATAGAAGTCATGCATCGTACACCTTACTCGACATACTAGGACAATTTGAGGGGCTCGACATACTAGGACAATTTGAGGGGCT

mRNA sequence

AAATAATTATGTACCGGAGCAGCACCGGTCTTCTGTCGCCGGCGAGTTGAAGAGGCAAAAAGCTAAGTTTTGACAATCCATTCATCGGCGGTTGTCTTCACCATTCGGCAGCTCTCCGGCTTACTCAACTTCAGAAGTTCCGACTTGCTCAACTGCTTGACCGCGGCACCAACGGAGGCGCCCATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTCATTTCCTCTCTCTTTCAGTCATTCGCAGTACGATGATGACGAATCCGATCAAAACTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCTGTCTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTCGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGACTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCGTCCGGACCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCCATTCGGAGCCTCCGAAGTCGGAATTTCATCCCTGACCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGAAAATTCGGGCTGAAGGAACCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCGACCGCCGACCCAGATGCCATTAGAAGAAGAATTAGGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAGATTATTGAGAAAGGACCCATAAGAGACTATTTGGGACGTCCATTATTGACGATGACTAACGATTCGAATCCGTGGTGGTCTGTTTTCAAGACAGCCATCTCAGACGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTAGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCACGAACACAACGAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAGATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCAGTAAGTGCTCCCATGGATGAACACTGCTATTGTAAGTACTGAAGTTCTCTTTTCCACACAACGTCACAATTCACTTGTAAACATTCTCTTTCGTTTTATTACCTTTTCATGAGATTCGAATAGGGTAAATAGTAAACAAAAAATGATCTTTTGTAGTATATTGAGATTGTCTCCTCTAAGCGCATCCTTCCTAAATTAAATGGGTATCAAATAACGAGATATATTCTATAGGTTGTATAAATGCGTTCTTGAATTCTAAGAAGTTATTTAGCTATCGACAATGAAATGTATATTACTCGAAATAGAAGTCATGCATCGTACACCTTACTCGACATACTAGGACAATTTGAGGGGCTCGACATACTAGGACAATTTGAGGGGCT

Coding sequence (CDS)

ATGGCGAACTTCCGTCTCCACTGTCGGCTCTTTTCTCTTGCAGTTTTTCTTTTTCTCTTCTCATTTCCTCTCTCTTTCAGTCATTCGCAGTACGATGATGACGAATCCGATCAAAACTCTCCAATCTCTCGCTTTCAACACTACCTCAGAATCAAAACCGCTCATCCGGATCCGGATTACGCCTCCGCTGTCTCCTTTCTCGAATCTCAAGCACTGGAAATTGGTCTCCATACGAAGATTCTTGAGTTTGTTCCAGGTAAACCTCTTCTCCTCGTGACCTGGAATGGATTGAATCCTTCTTTGCCCTCGATTTTACTCAACTCTCACATGGACTCTGTCCCCGCTGAGCCCTCTAAATGGCTCTATCCGCCGTTCTCCGCCGTCCGGACCTCCGATGGCAAGATCTTCGCCCGTGGTTCTCAAGATGATAAATGTATAGCTATCCAGTATCTCGAAGCCATTCGGAGCCTCCGAAGTCGGAATTTCATCCCTGACCGTACGATTCACATCTCGTATGTGCCGGATGAGGAGATTGGGGGTTTTGATGGAGCTGCGAAGTTCGTCTTGTCGAAGGAGTTTAAGGAGTTGAATGTTGGATTCATGATGGACGAAGGACAGGCGTCGCCGGGAGATGAGTTTAGGGTTTTTTACGCCGATAGATCGCCGTGGGGGCTGAAAATTCGGGCTGAAGGAACCCCTGGACATGGTTCTAGAATGTACGATAACAGTGCTATGGAGAATTTGATGAAGAGCGTTGAGATTATCAGTAAGTTTAGAGAGAGCCAATTCGATATGGTGAAGGCCGGTGAAGCTGCTAATTCAGAAGTCATCTCTGTCAATCCTGTTTTCGTCAACGCCGGAATTCCCTCCCCCACTGGATTTGTGATGAACATGCAACCTTCGGAGGCGGAAGCCGGTTTCGATCTCAGAATACCGCCGACCGCCGACCCAGATGCCATTAGAAGAAGAATTAGGGAGGAATGGGCTCCAGCTCGACGGAACTTGACGTCTCAGATTATTGAGAAAGGACCCATAAGAGACTATTTGGGACGTCCATTATTGACGATGACTAACGATTCGAATCCGTGGTGGTCTGTTTTCAAGACAGCCATCTCAGACGCTGGAGGTAAGCTTTCTAGGCCCGAAATCTTGGCTTCGACGACTGATGCACGCTTCATGAGACAGTTAGGCATTCCTGTTCTTGGTTTCTCCCCAATGAGTAACACTCCTATCTTATTGCACGAACACAACGAGTTCTTGAAGGACACCGTCTTCTTAAGGGGCATAAAGATTTATGAGTCCATAATCAGTGCCTTGAGTTCCTTTGTAGCGGATGCATCCCAGTAA

Protein sequence

MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASQ

Homology

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BLAST of CmoCh04G024620 vs. ExPASy Swiss-Prot
Match: Q99JW2 (Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.5e-93
Identity = 174/409 (42.54%), Postives = 248/409 (60.64%), Query Frame = 0


Query: 34  DESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVT 93
           D   ++  ++ F+ YLRI T  P+PDY  A++FLE +A ++GL  + +E VPG  + ++T
Sbjct: 5   DPESEHPSVTLFRQYLRICTVQPNPDYGGAITFLEERARQLGLSCQKIEVVPGFVITVLT 64

Query: 94  WNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEA 153
           W G NPSLPSILLNSH D VP     W + PF A + S+G I+ARGSQD K ++IQYLEA
Sbjct: 65  WPGTNPSLPSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGSQDMKSVSIQYLEA 124

Query: 154 IRSLRSRNFIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEF 213
           +R L+S      RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F
Sbjct: 125 VRRLKSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAF 184

Query: 214 RVFYADRSPWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-EAA 273
            VFY++RSPW +++ + G PGH SR  +++A E L K +  I  FRE +   ++A     
Sbjct: 185 TVFYSERSPWWVRVTSTGKPGHASRFIEDTAAEKLHKVISSILAFREKERQRLQANPHLK 244

Query: 274 NSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIREEWAPAR 333
              V SVN   +  G+        N+ P+   A FD R+ P  D  A  ++++     A 
Sbjct: 245 EGAVTSVNLTKLEGGV------AYNVVPATMSASFDFRVAPDVDMKAFEKQLQRWCQEAG 304

Query: 334 RNLTSQIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARF 393
             +T +  +K         P +T T+DS+PWW+ F  A   A      PEI  + TD+R+
Sbjct: 305 EGVTFEFAQK------FTEPRMTPTDDSDPWWAAFSGACK-AMNLTLEPEIFPAATDSRY 364

Query: 394 MRQLGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSS 442
           +R +GIP LGFSPM+ TP+LLH+HNE L + +FLRG+ IY  ++SAL+S
Sbjct: 365 IRAVGIPALGFSPMNRTPVLLHDHNERLHEDIFLRGVDIYTGLLSALAS 400

[Close]

BLAST of CmoCh04G024620 vs. ExPASy Swiss-Prot
Match: P37111 (Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2)

HSP 1 Score: 342.0 bits (876), Expect = 9.9e-93
Identity = 171/411 (41.61%), Postives = 250/411 (60.83%), Query Frame = 0


Query: 38  QNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGL 97
           ++  ++ F+ YLRI+T  P+PDY +AV+FLE +A ++GL  + +E VPG  + ++TW G 
Sbjct: 9   EHPSVTLFRQYLRIRTVQPEPDYGAAVAFLEERARQLGLGCQKVEVVPGHVVTVLTWPGT 68

Query: 98  NPSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSL 157
           NP+L SILLNSH D VP     W + PF   + +DG I+ RG+QD KC++IQYLEA+R L
Sbjct: 69  NPTLSSILLNSHTDVVPVFKEHWSHDPFEGFKDADGYIYGRGAQDMKCVSIQYLEAVRRL 128

Query: 158 RSRNFIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFY 217
           +       RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG ASP D F VFY
Sbjct: 129 KVEGHHFPRTIHMTFVPDEEVGGHQGMELFVKRPEFQALRAGFALDEGLASPTDAFTVFY 188

Query: 218 ADRSPWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVI 277
           ++RSPW L++ + G PGHGSR  +++A E L K +  I  FRE +   +++ +     V 
Sbjct: 189 SERSPWWLRVTSTGKPGHGSRFIEDTAAEKLHKVINSILAFREKEKQRLQSNQLKPGAVT 248

Query: 278 SVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTS 337
           SVN   +  G+        N+ P+   A FD R+ P  D  A   +++     A   +T 
Sbjct: 249 SVNLTMLEGGV------AYNVVPATMSACFDFRVAPDVDLKAFEEQLQSWCQAAGEGVTF 308

Query: 338 QIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLG 397
           + ++K           +T T+DS+PWW+ F     D    L   EI  ++TDAR++R  G
Sbjct: 309 EFVQKWM------ETQVTSTDDSDPWWAAFSGVFKDMKLALEL-EICPASTDARYIRAAG 368

Query: 398 IPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASQ 449
           +P LGFSPM++TP+LLH+H+E L + VFLRG+ IY  ++SAL+S  A  S+
Sbjct: 369 VPALGFSPMNHTPVLLHDHDERLHEAVFLRGVDIYTQLLSALASVPALPSE 406

[Close]

BLAST of CmoCh04G024620 vs. ExPASy Swiss-Prot
Match: Q6AYS7 (Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 4.2e-91
Identity = 172/408 (42.16%), Postives = 247/408 (60.54%), Query Frame = 0


Query: 38  QNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGL 97
           ++  ++ F+ YLRI T  P+PDY SAV+FLE +A ++GL  + +E  PG  + ++TW G 
Sbjct: 9   EHPSVTLFRQYLRICTVQPNPDYGSAVTFLEERARQLGLSCQKIEVAPGYVITVLTWPGT 68

Query: 98  NPSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSL 157
           NP L SILLNSH D VP     W + PF A + S+G I+ARG+QD K ++IQYLEA+R L
Sbjct: 69  NPLLHSILLNSHTDVVPVFKEHWHHDPFEAFKDSEGYIYARGAQDMKSVSIQYLEAVRRL 128

Query: 158 RSRNFIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFY 217
           +S      RTIH+++VPDEE+GG  G   FV   EF+ L  GF +DEG A+P D F VFY
Sbjct: 129 KSEGHRFPRTIHMTFVPDEEVGGHKGMELFVKRPEFQALRAGFALDEGLANPTDAFTVFY 188

Query: 218 ADRSPWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-EAANSEV 277
           ++RSPW +++ + G PGH SR  +++A E L K V  I  FRE +   ++A        V
Sbjct: 189 SERSPWWIRVTSTGKPGHASRFIEDTAAEKLHKVVNSILAFREKERQRLQANPHLKEGAV 248

Query: 278 ISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLT 337
            SVN   +  G+        N+ P+   A FD R+ P  D  A  ++++     A   +T
Sbjct: 249 TSVNLTKLEGGV------AYNVVPATMSACFDFRVAPDVDMKAFEKQLQSWCQEAGEGVT 308

Query: 338 SQIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL 397
            +  +K         P +T T+D++PWW+ F  A  +    L  PEI  + TD+R++R +
Sbjct: 309 FEFAQK------FTEPRMTPTDDTDPWWAAFSGACKEMNLTL-EPEIFPAATDSRYIRAV 368

Query: 398 GIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           GIP LGFSPM+ TP+LLH+HNE L + VFLRG+ IY  +++AL+S  A
Sbjct: 369 GIPALGFSPMNRTPVLLHDHNERLHEAVFLRGVDIYTRLVAALASVPA 403

[Close]

BLAST of CmoCh04G024620 vs. ExPASy Swiss-Prot
Match: Q5RFB0 (Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2)

HSP 1 Score: 335.9 bits (860), Expect = 7.1e-91
Identity = 172/413 (41.65%), Postives = 247/413 (59.81%), Query Frame = 0


Query: 37  DQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNG 96
           +++  ++ F+ YLRI+T  P PDY +AV+F E +A ++GL  + +E  PG  + ++TW G
Sbjct: 8   EEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEERARQLGLGCQKVEVAPGYVVTVLTWPG 67

Query: 97  LNPSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRS 156
            NP+L SILLNSH D VP     W + PF A + S+G I+ARG+QD KCI+IQYLEA+R 
Sbjct: 68  TNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCISIQYLEAVRR 127

Query: 157 LRSRNFIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVF 216
           L+       RTIH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VF
Sbjct: 128 LKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVF 187

Query: 217 YADRSPWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-EAANSE 276
           Y++RSPW +++ + G PGH SR  +++A E L K V  I  FRE ++  +++        
Sbjct: 188 YSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVSSILAFREKEWQRLQSNPHLKEGS 247

Query: 277 VISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNL 336
           V SVN   +  G+        N+ P+   A FD R+ P  D  A   +++     A   +
Sbjct: 248 VTSVNLTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGV 307

Query: 337 TSQIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQ 396
           T +  +K         P +T T+DSNPWW+ F     D    L  PEI+ + TD R++R 
Sbjct: 308 TLEFAQKWM------HPQVTPTDDSNPWWAAFSRVCKDMKLTL-EPEIMPAATDNRYIRA 367

Query: 397 LGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVADASQ 449
           +GIP LGFSPM+ TP+LLH+H+E L + VFLRG+ IY  ++ AL+S  A  S+
Sbjct: 368 VGIPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPALPSE 407

[Close]

BLAST of CmoCh04G024620 vs. ExPASy Swiss-Prot
Match: Q03154 (Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 2.1e-90
Identity = 169/409 (41.32%), Postives = 244/409 (59.66%), Query Frame = 0


Query: 37  DQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNG 96
           +++  ++ F+ YLRI+T  P PDY +AV+F E  A ++GL  + +E  PG  + ++TW G
Sbjct: 8   EEHPSVTLFRQYLRIRTVQPKPDYGAAVAFFEETARQLGLGCQKVEVAPGYVVTVLTWPG 67

Query: 97  LNPSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRS 156
            NP+L SILLNSH D VP     W + PF A + S+G I+ARG+QD KC++IQYLEA+R 
Sbjct: 68  TNPTLSSILLNSHTDVVPVFKEHWSHDPFEAFKDSEGYIYARGAQDMKCVSIQYLEAVRR 127

Query: 157 LRSRNFIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVF 216
           L+       RTIH+++VPDEE+GG  G   FV   EF  L  GF +DEG A+P D F VF
Sbjct: 128 LKVEGHRFPRTIHMTFVPDEEVGGHQGMELFVQRPEFHALRAGFALDEGIANPTDAFTVF 187

Query: 217 YADRSPWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAG-EAANSE 276
           Y++RSPW +++ + G PGH SR  +++A E L K V  I  FRE ++  +++        
Sbjct: 188 YSERSPWWVRVTSTGRPGHASRFMEDTAAEKLHKVVNSILAFREKEWQRLQSNPHLKEGS 247

Query: 277 VISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNL 336
           V SVN   +  G+        N+ P+   A FD R+ P  D  A   +++     A   +
Sbjct: 248 VTSVNLTKLEGGV------AYNVIPATMSASFDFRVAPDVDFKAFEEQLQSWCQAAGEGV 307

Query: 337 TSQIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQ 396
           T +  +K         P +T T+DSNPWW+ F     D    L  PEI+ + TD R++R 
Sbjct: 308 TLEFAQKWM------HPQVTPTDDSNPWWAAFSRVCKDMNLTL-EPEIMPAATDNRYIRA 367

Query: 397 LGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSFVA 445
           +G+P LGFSPM+ TP+LLH+H+E L + VFLRG+ IY  ++ AL+S  A
Sbjct: 368 VGVPALGFSPMNRTPVLLHDHDERLHEAVFLRGVDIYTRLLPALASVPA 403

[Close]

BLAST of CmoCh04G024620 vs. ExPASy TrEMBL
Match: A0A6J1HLT6 (aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.1e-259
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQ 449
           KDTVFLRGIKIYESIISALSSFVADASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of CmoCh04G024620 vs. ExPASy TrEMBL
Match: A0A6J1JRT3 (aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.3e-254
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLF FPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIR+LRSRNFIPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRAEG PGHGSRMY
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRI EEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQ 449
           KDTVFLRGIKIYESIISALSSFVADASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of CmoCh04G024620 vs. ExPASy TrEMBL
Match: A0A5A7URV3 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G001820 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.9e-225
Identity = 381/447 (85.23%), Postives = 418/447 (93.51%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           M+NFRLH R FS+ ++L L SFPLSFSHSQY DDESDQ+SP+SRFQ YLRIKTAHPDPDY
Sbjct: 85  MSNFRLHHRFFSVTLYLLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDPDY 144

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL SQA EIGLHT++LEF PGKPLLLVTW G NPSLPS+LLNSHMDSVPAEPSKW
Sbjct: 145 ASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPSKW 204

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           L+PPFSAVRTSDGKIFARGSQDDKCIAIQY+EAIR+LR+R+F+P RT+HISYVPDEEIGG
Sbjct: 205 LHPPFSAVRTSDGKIFARGSQDDKCIAIQYIEAIRNLRNRDFVPVRTVHISYVPDEEIGG 264

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
            DG AKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW L I+A+GTPGHGSRMY
Sbjct: 265 SDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSRMY 324

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIIS+FRESQF++VKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 325 DNSAMENLMKSVEIISRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 384

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLR+PPTADPDA+RRRI EEWAPA RN+T QIIEKGP+RDYLG PL+TMTNDS
Sbjct: 385 SEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTNDS 444

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNEFL
Sbjct: 445 NPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFL 504

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
            D+VF+RGIK+YES+IS+LSSF  D S
Sbjct: 505 SDSVFIRGIKVYESLISSLSSFQEDVS 531

[Close]

BLAST of CmoCh04G024620 vs. ExPASy TrEMBL
Match: A0A5D3BIQ8 (Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G001910 PE=4 SV=1)

HSP 1 Score: 790.4 bits (2040), Expect = 3.9e-225
Identity = 381/447 (85.23%), Postives = 417/447 (93.29%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           M+NFRLH R FS+ ++L L SFPLSFSHSQY DDESDQ+SP+SRFQ YLRIKTAHPDPDY
Sbjct: 1   MSNFRLHHRFFSVTLYLLLLSFPLSFSHSQYVDDESDQDSPVSRFQRYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAV+FL SQA EIGLHT++LEF PGKPLLLVTW G NPSLPS+LLNSHMDSVPAEPSKW
Sbjct: 61  ASAVAFLRSQAQEIGLHTEVLEFGPGKPLLLVTWYGSNPSLPSVLLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           L+PPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIR+LR+R+F+P RT+HISYVPDEEIGG
Sbjct: 121 LHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTVHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
            DG AKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW L I+A+GTPGHGSRMY
Sbjct: 181 SDGVAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEI+S+FRESQF++VKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIVSRFRESQFEIVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLR+PPTADPDA+RRRI EEWAPA RN+T QIIEKGP+RDYLG PL+TMTNDS
Sbjct: 301 SEAEAGFDLRLPPTADPDAMRRRIAEEWAPAWRNMTYQIIEKGPLRDYLGSPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNEFL
Sbjct: 361 NPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
            D+VF+RGIK+YES+IS LSSF  D S
Sbjct: 421 SDSVFIRGIKVYESLISTLSSFQEDVS 447

[Close]

BLAST of CmoCh04G024620 vs. ExPASy TrEMBL
Match: A0A0A0LRI1 (M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005600 PE=4 SV=1)

HSP 1 Score: 778.1 bits (2008), Expect = 2.0e-221
Identity = 380/449 (84.63%), Postives = 417/449 (92.87%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVF--LFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDP 60
           M+NF LH RLFS+ ++  L L SFPLSFS SQY DDE DQ+SP+SRFQ YLRIKTAHPDP
Sbjct: 1   MSNFHLHYRLFSVTLYLLLLLLSFPLSFSQSQYVDDEPDQDSPVSRFQRYLRIKTAHPDP 60

Query: 61  DYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPS 120
           DYASAV+FL SQA EIGLHT+ILEFV GKPLLLVTW GL+PSLPSILLNSHMDSVPAEPS
Sbjct: 61  DYASAVAFLRSQAQEIGLHTQILEFVTGKPLLLVTWYGLDPSLPSILLNSHMDSVPAEPS 120

Query: 121 KWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEI 180
           KW++PPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIR+LR+R+F+P RTIHISYVPDEEI
Sbjct: 121 KWVHPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRNRDFVPVRTIHISYVPDEEI 180

Query: 181 GGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSR 240
           GG DGAAKFV SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPW L I+A+G+PGHGSR
Sbjct: 181 GGSDGAAKFVQSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWSLIIKAKGSPGHGSR 240

Query: 241 MYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNM 300
           MYDNSAMENLMKSVEI+++FRESQF++VKAGEAANSEVISVNPVFVNAG PSPTGFVMNM
Sbjct: 241 MYDNSAMENLMKSVEIMTRFRESQFEIVKAGEAANSEVISVNPVFVNAGTPSPTGFVMNM 300

Query: 301 QPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTN 360
           QPSEAEAGFDLR+PPTADPDA+RRRI EEWAPARRN+T QI+EKGPIRDYLG PL+TMTN
Sbjct: 301 QPSEAEAGFDLRLPPTADPDAMRRRIAEEWAPARRNMTYQILEKGPIRDYLGSPLMTMTN 360

Query: 361 DSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNE 420
            SNPWW+VFK AISDAGGKLS+PEILA+TTDARFMRQ+ IPVLGFSPM NTPILLH+HNE
Sbjct: 361 ASNPWWAVFKAAISDAGGKLSKPEILATTTDARFMRQMRIPVLGFSPMINTPILLHDHNE 420

Query: 421 FLKDTVFLRGIKIYESIISALSSFVADAS 448
           FL D+VF+RGIK+YES+ISALSSF  D S
Sbjct: 421 FLSDSVFIRGIKVYESLISALSSFQEDVS 449

[Close]

BLAST of CmoCh04G024620 vs. NCBI nr
Match: XP_022964785.1 (aminoacylase-1 [Cucurbita moschata])

HSP 1 Score: 903.7 bits (2334), Expect = 6.5e-259
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQ 449
           KDTVFLRGIKIYESIISALSSFVADASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of CmoCh04G024620 vs. NCBI nr
Match: KAG6602086.1 (Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 888.6 bits (2295), Expect = 2.2e-254
Identity = 441/447 (98.66%), Postives = 443/447 (99.11%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLFSF LSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIR+LRSRNF+PDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRAEGTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRI EEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
           KDTVFLRGIKIYESIISALSSFVADAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of CmoCh04G024620 vs. NCBI nr
Match: KAG7032791.1 (Aminoacylase-1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 888.6 bits (2295), Expect = 2.2e-254
Identity = 441/447 (98.66%), Postives = 443/447 (99.11%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLFSF LSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFSFSLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIR+LRSRNF+PDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRAEGTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRI EEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQL IPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLAIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
           KDTVFLRGIKIYESIISALSSFVADAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of CmoCh04G024620 vs. NCBI nr
Match: XP_022991055.1 (aminoacylase-1 [Cucurbita maxima])

HSP 1 Score: 887.5 bits (2292), Expect = 4.8e-254
Identity = 439/448 (97.99%), Postives = 443/448 (98.88%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAVFLFLF FPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVFLFLFLFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSA+RTSDGKIFARGSQDDKCIAIQYLEAIR+LRSRNFIPDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSALRTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFIPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
           FDGA+KFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRAEG PGHGSRMY
Sbjct: 181 FDGASKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGAPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGE ANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGETANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRI EEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADASQ 449
           KDTVFLRGIKIYESIISALSSFVADASQ
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADASQ 448

[Close]

BLAST of CmoCh04G024620 vs. NCBI nr
Match: XP_023553812.1 (aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553821.1 aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553828.1 aminoacylase-1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 876.7 bits (2264), Expect = 8.5e-251
Identity = 436/447 (97.54%), Postives = 440/447 (98.43%), Query Frame = 0


Query: 1   MANFRLHCRLFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDY 60
           MANFRLHCRLFSLAV LFLFSFPLSFS S+Y DDESDQNS ISRFQHYLRIKTAHPDPDY
Sbjct: 1   MANFRLHCRLFSLAVILFLFSFPLSFSDSKYVDDESDQNSSISRFQHYLRIKTAHPDPDY 60

Query: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120
           ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW
Sbjct: 61  ASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKW 120

Query: 121 LYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGG 180
           LYPPFSAV TSDGKIFARGSQDDKCIAIQYLEAIR+LRSRNF+PDRTIHISYVPDEEIGG
Sbjct: 121 LYPPFSAVLTSDGKIFARGSQDDKCIAIQYLEAIRNLRSRNFVPDRTIHISYVPDEEIGG 180

Query: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMY 240
           FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGL IRAEGTPGHGSRMY
Sbjct: 181 FDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLIIRAEGTPGHGSRMY 240

Query: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300
           DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP
Sbjct: 241 DNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQP 300

Query: 301 SEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDS 360
           SEAEAGFDLRIPPTADPDAIRRRI EEWAPARRNLTSQIIEKGPIRDYLGRPL+TMTNDS
Sbjct: 301 SEAEAGFDLRIPPTADPDAIRRRIVEEWAPARRNLTSQIIEKGPIRDYLGRPLMTMTNDS 360

Query: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420
           NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL
Sbjct: 361 NPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFL 420

Query: 421 KDTVFLRGIKIYESIISALSSFVADAS 448
           KDTVFLRGIKIYESIISALSSFVADAS
Sbjct: 421 KDTVFLRGIKIYESIISALSSFVADAS 447

[Close]

BLAST of CmoCh04G024620 vs. TAIR 10
Match: AT1G44820.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 631.7 bits (1628), Expect = 4.4e-181
Identity = 296/439 (67.43%), Postives = 364/439 (82.92%), Query Frame = 0


Query: 10  LFSLAVFLFLFSFPLSFSHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLES 69
           L++L +F  +FS   S          S++++PI+RFQ YLR  TAHP+P+Y + +SFL +
Sbjct: 7   LWTLIIFSIIFSLQSS---------SSEEDTPITRFQQYLRFNTAHPNPNYTAPISFLIN 66

Query: 70  QALEIGLHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSAVR 129
           QA  IGL TK +EF+ GKP+LL+TW G NP+LPSIL NSH+DSVPAE  KW YPPFSA +
Sbjct: 67  QAQSIGLTTKTIEFISGKPILLITWLGSNPNLPSILFNSHLDSVPAESEKWTYPPFSAHK 126

Query: 130 TSDGKIFARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGGFDGAAKFVL 189
           T DG I+ARG+QDDKCI +QYLE+IR+L+SR F P RTIHISYVP+EEIGGFDG  KF  
Sbjct: 127 TIDGHIYARGAQDDKCIGVQYLESIRNLKSRGFSPLRTIHISYVPEEEIGGFDGMMKFAA 186

Query: 190 SKEFKELNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMYDNSAMENLM 249
           S EFK+LN+GF MDEGQA+PGDEFRVFYADR PW   I+AEG PGHG+++YDNSAMENLM
Sbjct: 187 SSEFKDLNLGFAMDEGQANPGDEFRVFYADRVPWHFVIKAEGIPGHGAKLYDNSAMENLM 246

Query: 250 KSVEIISKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDL 309
           KSVE+IS+FRESQFD VKAG+AA SEVISVNPV++ AG P+ TGFVMNMQPSEAEAG+DL
Sbjct: 247 KSVELISRFRESQFDFVKAGKAAYSEVISVNPVYLKAGTPTTTGFVMNMQPSEAEAGYDL 306

Query: 310 RIPPTADPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKT 369
           R+PP ADPD +++RI EEWAP+ RN+T  I EKG +RD+LGRP++T  NDSNPWWS+FK 
Sbjct: 307 RLPPMADPDVMKKRIAEEWAPSIRNMTYSIQEKGKLRDHLGRPIMTPVNDSNPWWSIFKQ 366

Query: 370 AISDAGGKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGI 429
           A+   GGKL++PEILASTTD+RF+R LGIP  GFSPM+NTPILLH+HNEFLKDTVF++GI
Sbjct: 367 AVEAMGGKLAKPEILASTTDSRFIRTLGIPTFGFSPMTNTPILLHDHNEFLKDTVFMKGI 426

Query: 430 KIYESIISALSSFVADASQ 449
           ++YES+ISALSSF  +++Q
Sbjct: 427 EVYESVISALSSFEGESAQ 436

[Close]

BLAST of CmoCh04G024620 vs. TAIR 10
Match: AT1G44180.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 623.6 bits (1607), Expect = 1.2e-178
Identity = 296/429 (69.00%), Postives = 362/429 (84.38%), Query Frame = 0


Query: 17  LFLFSFPLSF-SHSQYDDDESDQNSPISRFQHYLRIKTAHPDPDYASAVSFLESQALEIG 76
           LFLFSF  S  SHS+    E ++++PI+RFQ YLR  TAHP+P+Y + +SFL  QA  IG
Sbjct: 10  LFLFSFIFSLQSHSK----EEEEDTPITRFQQYLRFNTAHPNPNYTAPISFLLDQARSIG 69

Query: 77  LHTKILEFVPGKPLLLVTWNGLNPSLPSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKI 136
           L ++ +EFVPGKP+LL+TW G N +L SIL NSH+DSVPAE  KW++PPFSA RT DG I
Sbjct: 70  LTSRTIEFVPGKPVLLLTWLGSNLNLSSILFNSHLDSVPAESEKWIHPPFSAHRTIDGHI 129

Query: 137 FARGSQDDKCIAIQYLEAIRSLRSRNFIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKE 196
           +ARG+QDDKCI +QYLEAIR+L+SR+F P RTIHISYVP+EEIGGF G  KF  S EFK+
Sbjct: 130 YARGAQDDKCIGVQYLEAIRNLKSRSFSPLRTIHISYVPEEEIGGFGGMMKFAASSEFKD 189

Query: 197 LNVGFMMDEGQASPGDEFRVFYADRSPWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEII 256
           LN+GF+MDEGQASPGDEFRVFYA+R+PW L IRA G PGHG+++YDNSAMENLMKSVE+I
Sbjct: 190 LNLGFVMDEGQASPGDEFRVFYAERTPWHLAIRANGMPGHGAKLYDNSAMENLMKSVELI 249

Query: 257 SKFRESQFDMVKAGEAANSEVISVNPVFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTA 316
           S+FRESQFD VKAG+AANSEVISVNPV++ AG PS TGFVMNMQPSEAE G+DLR+PP A
Sbjct: 250 SRFRESQFDFVKAGKAANSEVISVNPVYLKAGTPSTTGFVMNMQPSEAEVGYDLRLPPMA 309

Query: 317 DPDAIRRRIREEWAPARRNLTSQIIEKGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAG 376
           DP A+++RI EEWAP+ RN+T  + ++G + D+LGRP++T TND+NPWWS+FK A+   G
Sbjct: 310 DPVALKKRIAEEWAPSIRNMTYTLKQQGKLTDHLGRPIMTTTNDTNPWWSIFKQAVEATG 369

Query: 377 GKLSRPEILASTTDARFMRQLGIPVLGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESI 436
           GKL++PEIL STTD+RF+R LGIPVLGFSPM NTPIL+H+HNEFLKDTVF++GI +YES+
Sbjct: 370 GKLAKPEILISTTDSRFIRTLGIPVLGFSPMINTPILVHDHNEFLKDTVFMKGIGVYESV 429

Query: 437 ISALSSFVA 445
           ISALSSF A
Sbjct: 430 ISALSSFEA 434

[Close]

BLAST of CmoCh04G024620 vs. TAIR 10
Match: AT4G38220.2 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 481.1 bits (1237), Expect = 9.7e-136
Identity = 223/402 (55.47%), Postives = 298/402 (74.13%), Query Frame = 0


Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           +SRFQ YLRI T  P+P+Y  AV F+ SQA  + L ++ +EFV GKPLLL+ W G +P+L
Sbjct: 25  VSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSDPTL 84

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRN 161
           P+ LLNSH D VP E SKW + P  A     G I+ARGSQD KC+ +QYLEAIR L++  
Sbjct: 85  PAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQASG 144

Query: 162 FIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
           F P R++++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RVFY +RS
Sbjct: 145 FKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERS 204

Query: 222 PWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNP 281
           PW L I+A+G PGHG+++YDNSAMENL+KS+E I +FR SQFD++KAG  A  +V+SVN 
Sbjct: 205 PWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVSVNM 264

Query: 282 VFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIE 341
            F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+ EEWAPA RN++    E
Sbjct: 265 AFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMS---FE 324

Query: 342 KGPIRDYL-GRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPV 401
            G  +  L G+  LT  +DSNPWW + + A+ +AGG+ S+PEI  ++TDAR+ R+ G+P 
Sbjct: 325 LGQFKQKLTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGVPA 384

Query: 402 LGFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSF 443
            GFSP+SNTP LLH+HNE+L    +L+GI++Y SII A +S+
Sbjct: 385 FGFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASY 423

[Close]

BLAST of CmoCh04G024620 vs. TAIR 10
Match: AT4G38220.1 (Peptidase M20/M25/M40 family protein )

HSP 1 Score: 480.7 bits (1236), Expect = 1.3e-135
Identity = 221/401 (55.11%), Postives = 297/401 (74.06%), Query Frame = 0


Query: 42  ISRFQHYLRIKTAHPDPDYASAVSFLESQALEIGLHTKILEFVPGKPLLLVTWNGLNPSL 101
           +SRFQ YLRI T  P+P+Y  AV F+ SQA  + L ++ +EFV GKPLLL+ W G +P+L
Sbjct: 25  VSRFQEYLRINTVQPNPEYYKAVDFIISQAKPLSLESQTIEFVKGKPLLLLKWVGSDPTL 84

Query: 102 PSILLNSHMDSVPAEPSKWLYPPFSAVRTSDGKIFARGSQDDKCIAIQYLEAIRSLRSRN 161
           P+ LLNSH D VP E SKW + P  A     G I+ARGSQD KC+ +QYLEAIR L++  
Sbjct: 85  PAFLLNSHTDVVPFEDSKWTHHPLQAHMDHHGDIYARGSQDMKCVGMQYLEAIRKLQASG 144

Query: 162 FIPDRTIHISYVPDEEIGGFDGAAKFVLSKEFKELNVGFMMDEGQASPGDEFRVFYADRS 221
           F P R++++S+VPDEEIGG DGA KF  S+ FK LN+  ++DEG  SP + +RVFY +RS
Sbjct: 145 FKPLRSVYLSFVPDEEIGGHDGAEKFAESQLFKSLNIAIVLDEGLPSPTESYRVFYGERS 204

Query: 222 PWGLKIRAEGTPGHGSRMYDNSAMENLMKSVEIISKFRESQFDMVKAGEAANSEVISVNP 281
           PW L I+A+G PGHG+++YDNSAMENL+KS+E I +FR SQFD++KAG  A  +V+SVN 
Sbjct: 205 PWWLVIKAKGPPGHGAKLYDNSAMENLLKSIESIRRFRASQFDLLKAGGIAEGDVVSVNM 264

Query: 282 VFVNAGIPSPTGFVMNMQPSEAEAGFDLRIPPTADPDAIRRRIREEWAPARRNLTSQIIE 341
            F+ AG PSPTGFVMN+QPSEAEAGFD+R+PP+ D +A+ RR+ EEWAPA RN++ +  +
Sbjct: 265 AFLKAGTPSPTGFVMNLQPSEAEAGFDIRVPPSVDAEALERRLVEEWAPAARNMSFEFKQ 324

Query: 342 KGPIRDYLGRPLLTMTNDSNPWWSVFKTAISDAGGKLSRPEILASTTDARFMRQLGIPVL 401
           K       G+  LT  +DSNPWW + + A+ +AGG+ S+PEI  ++TDAR+ R+ G+P  
Sbjct: 325 K-----LTGKQFLTAADDSNPWWGLLENAVKEAGGRTSKPEIFPASTDARYFRKAGVPAF 384

Query: 402 GFSPMSNTPILLHEHNEFLKDTVFLRGIKIYESIISALSSF 443
           GFSP+SNTP LLH+HNE+L    +L+GI++Y SII A +S+
Sbjct: 385 GFSPISNTPSLLHDHNEYLGKAEYLKGIEVYVSIIKAYASY 420

The following BLAST results are available for this feature:

BLAST of CmoCh04G024620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q99JW2	1.5e-93	42.54	Aminoacylase-1 OS=Mus musculus OX=10090 GN=Acy1 PE=1 SV=1	[more]
P37111	9.9e-93	41.61	Aminoacylase-1 OS=Sus scrofa OX=9823 GN=ACY1 PE=1 SV=2	[more]
Q6AYS7	4.2e-91	42.16	Aminoacylase-1A OS=Rattus norvegicus OX=10116 GN=Acy1a PE=1 SV=1	[more]
Q5RFB0	7.1e-91	41.65	Aminoacylase-1 OS=Pongo abelii OX=9601 GN=ACY1 PE=2 SV=2	[more]
Q03154	2.1e-90	41.32	Aminoacylase-1 OS=Homo sapiens OX=9606 GN=ACY1 PE=1 SV=1	[more]

BLAST of CmoCh04G024620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HLT6	3.1e-259	100.00	aminoacylase-1 OS=Cucurbita moschata OX=3662 GN=LOC111464784 PE=4 SV=1	[more]
A0A6J1JRT3	2.3e-254	97.99	aminoacylase-1 OS=Cucurbita maxima OX=3661 GN=LOC111487760 PE=4 SV=1	[more]
A0A5A7URV3	3.9e-225	85.23	Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G00182...	[more]
A0A5D3BIQ8	3.9e-225	85.23	Aminoacylase-1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00191...	[more]
A0A0A0LRI1	2.0e-221	84.63	M20_dimer domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G005600 P...	[more]

BLAST of CmoCh04G024620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022964785.1	6.5e-259	100.00	aminoacylase-1 [Cucurbita moschata]	[more]
KAG6602086.1	2.2e-254	98.66	Aminoacylase-1, partial [Cucurbita argyrosperma subsp. sororia]	[more]
KAG7032791.1	2.2e-254	98.66	Aminoacylase-1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022991055.1	4.8e-254	97.99	aminoacylase-1 [Cucurbita maxima]	[more]
XP_023553812.1	8.5e-251	97.54	aminoacylase-1 [Cucurbita pepo subsp. pepo] >XP_023553821.1 aminoacylase-1 [Cucu...	[more]

BLAST of CmoCh04G024620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G44820.1	4.4e-181	67.43	Peptidase M20/M25/M40 family protein	[more]
AT1G44180.1	1.2e-178	69.00	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.2	9.7e-136	55.47	Peptidase M20/M25/M40 family protein	[more]
AT4G38220.1	1.3e-135	55.11	Peptidase M20/M25/M40 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002933	Peptidase M20	PFAM	PF01546	Peptidase_M20	coord: 105..437 e-value: 1.4E-32 score: 113.7
None	No IPR available	GENE3D	3.30.70.360		coord: 225..333 e-value: 1.9E-10 score: 42.5
None	No IPR available	GENE3D	3.40.630.10	Zn peptidases	coord: 32..224 e-value: 1.2E-53 score: 183.6
None	No IPR available	GENE3D	1.10.150.900		coord: 361..442 e-value: 1.9E-28 score: 100.0
None	No IPR available	PIRSF	PIRSF036696	ACY-1	coord: 31..444 e-value: 4.1E-168 score: 557.0
None	No IPR available	PANTHER	PTHR45892:SF3	PUTATIVE-RELATED	coord: 29..445
None	No IPR available	PANTHER	PTHR45892	AMINOACYLASE-1	coord: 29..445
None	No IPR available	CDD	cd05646	M20_AcylaseI_like	coord: 39..440 e-value: 0.0 score: 633.155
None	No IPR available	SUPERFAMILY	53187	Zn-dependent exopeptidases	coord: 37..440
IPR011650	Peptidase M20, dimerisation domain	PFAM	PF07687	M20_dimer	coord: 220..326 e-value: 3.7E-8 score: 33.2
IPR010159	N-acyl-L-amino-acid amidohydrolase	TIGRFAM	TIGR01880	TIGR01880	coord: 36..442 e-value: 2.9E-179 score: 593.9
IPR036264	Bacterial exopeptidase dimerisation domain	SUPERFAMILY	55031	Bacterial exopeptidase dimerisation domain	coord: 224..330

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmoCh04G024620.1	CmoCh04G024620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006520	cellular amino acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004046	aminoacylase activity
molecular_function	GO:0016787	hydrolase activity