CmoCh04G012350 (gene) Cucurbita moschata (Rifu) v1

Overview
NameCmoCh04G012350
Typegene
OrganismCucurbita moschata (Cucurbita moschata (Rifu) v1)
DescriptionTropinone reductase-like protein
LocationCmo_Chr04: 6278066 .. 6280750 (-)
RNA-Seq ExpressionCmoCh04G012350
SyntenyCmoCh04G012350
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AATCAATACAGCACAGACAAATAAATGTGAGATAAATTGGTTTTGGGATTTACAATTTGTGATTGGAAACAGCAAGATCTAGAGCTAATCCAAATTCAAAGATGGCGGAGGCAGGCGTCAGTTCCGGAAATGCCCGATGGTCTCTCAAGGGCTTCACAGCGCTCGTCACTGGCGGCACTCGTGGAATCGGGTCTGCTAAATTTCTAACTCTATTTCCTCTGAAATCCTCTTTCTGAGTTTGAATTTGGATTCTCTTCTGTTCTGTGTGCGATTTTGTGTGTGTTTTATAGTCGCGCTGTGGTGGAGGAACTGGCCGGTTTAGGCGTGTCGGTCCACACCTGTTCCCGGAACGAACTGGAGCTCAACCAGTGCTTGAAGGAATGGGAGGGCAAAGGCTTTGTGGTCAGTGGTTCGGTTTGCGATGCTTCTTCGAGAACACAGAGAGAGAGGCTCGTACAGGAAGTGGCGTCTGCTTTCAATGGCACCCTCAATATTCTGGTCAGTAATAGTATGCTTCGCCCATTTTAATTTTAGAGAAGCCATTTGCTAACAGTGTTGAAATTGGGTGTTGTTTGGTGCATGGCTTTGGGTTGGGAATTGAAGCCCTTATATCACTTACATTTTTACATTTCAATGCCATTCTAGTTCTGCGTGTACGTTAGCTTGGACGCTATGAAAGAAAACACAGATCTAATATATTAATATGTTGATGGCTTGAACTCCATGAAAGATAACAAAATGGCAGCAAAGTGAGACAAACTTTAGACCTTGACCGAAATGATGAATTGAAACCACTGAAAAGTGATATAAAGTAAGTCATTATAGGCAGGTGCTATAATTTTTCCATACTTGACGTCCACGAAATATTCAGTGCATTGCAATCAAACAATTGAAACTAGGAACAAGAAAGCCTTCTATTGACCTCAAGTGATTCAAACCCTCCATTTTGAAAGCAAGAACATCTCGTTGTTGGCTCCACAATGAAAAACATAATTCTAAGGAAGTTGCCTTCCAGGAATTCTAAACCTACTAGATATTGTTCTCTTTGGACTTTTCTTTTTGGATTTCGCCTCAAGGTTTTTAAAACGCATATGCTAGGGAGAGGTTTCCACACCCTTATAAAGAATGTTTTGTTCTCCTTTCCAACCGATGGGGGATCTTACTGTATTTTAGTGTAGGTAAATAATGTGGGAACAAACATCAGGAAGCCAAGTATTGAGTACAGTCTGGAAGAATTTTCGACATTGATGACTACGAACTTTGAATCAGTATTTCATCTATCCCAACTTGCTCATCCTCTTTTAAAAGCTTCTGGAAATGGAAGTATTGTGTTTGTTTCTTCGGTTGGTGGTCTCGTGAGCATTGGAAGTGGATCCATCTATGCAGCCTCTAAATGTGAGTTGCTATTGTAGAATTGTAGCTTATTTTCCTTCGTACGTTCTGAGAAGCTCAAAACTGTTCTTTTTTATTTTCTACTCTCAGCTGCAATGAATCAACTGACCAAAAATCTTGCTTGTGAATGGGCCAAGGACAATATCAGGAGCAACACTGTTGCACCCTGGTATACCAATACCCCCCTTGTAGAAAAGGTAATATTTTCAACAACTCCTGCCAGATAATTCACTCATATTAGTTTATTTTTTGCTCTCGTATCCTCTTTCTGTCTCCTCGTTTGATTCCTTCTTGTCATTAGACTGACTGACCAAGGCATGAGGCGCTCTGTCATTAATCTCAGTATCTGGGATGCTTTCAATCTGATGCATCTTTCTGTGAAATCTCTATGAATGTCAAATTGATTAATTGAGTCACCTCGGCAAAATATGACTTGGTAACTGTTGTTCAAGCTTGAAAACATATGGTCAATTAATGGCATTGTTCTTTCTGTCTTTCTTGTTTATCACGCTTCTGTTTTTGTGCCTTTTTTTTTCCCCTGATTGAAGGCTGCCCATGTAAAAAAAAAAAAAACATGCCCTTTCGTGCGGGTATGAGGATATGAATCTTAGTGTGCACGAACACGCAATTCCATATTTTTAATAGTGTACATTTTGATGCTGAAATTTATTTGTACCAACATTTTGCAATTCTATATCTTTGAGTCCGACATCTTTCCATCTTTAGAACCGAAGAACACTGTTGTACAATCTATAGAACAAATTCTGTTCAAATAACCTGGTCTGAGATTGTCCATCTTTGTTGCATCTTTTTTCAGTTCAACCCCTAAACCTGTTGAATGTTAACTAATAACAATGGTTATTATCGTCGTAGTTGCTTGATAATAAGACGTTCGTGGACAAGGTAGTCTCTCGAACTCCACTTGGGCGAATAGGGGAATCAAATGAAGTGTCCTCGTTGGTGGCATTCCTTTGCATGCCTGCTGCATCTTACATCACTGGACAGATTATTTCTGTTGATGGAGGGTTCACTGCCAATGGGTTTGAGTCTGGTATGAGACTAGAAAGCTTGTGAAACATGCTTCTGCTTCTTACATCACTGGGCAGACTATTTCCACAATAATCTTTTATGCTTATCATGTTTTTCTCAACTCTTTCAAGTTTTATGTAAACTCTATGTAATAATTCTAAATAATTTAATGTGCACAATCTTGAACTAAAAAAATAGTTTTAAAAGTTTTATTTTAGGGTCTTATGCACCATGGAAATATACTCTTAAAATTTATTGAGAAAA

mRNA sequence

AATCAATACAGCACAGACAAATAAATGTGAGATAAATTGGTTTTGGGATTTACAATTTGTGATTGGAAACAGCAAGATCTAGAGCTAATCCAAATTCAAAGATGGCGGAGGCAGGCGTCAGTTCCGGAAATGCCCGATGGTCTCTCAAGGGCTTCACAGCGCTCGTCACTGGCGGCACTCGTGGAATCGGTCGCGCTGTGGTGGAGGAACTGGCCGGTTTAGGCGTGTCGGTCCACACCTGTTCCCGGAACGAACTGGAGCTCAACCAGTGCTTGAAGGAATGGGAGGGCAAAGGCTTTGTGGTCAGTGGTTCGGTTTGCGATGCTTCTTCGAGAACACAGAGAGAGAGGCTCGTACAGGAAGTGGCGTCTGCTTTCAATGGCACCCTCAATATTCTGGTAAATAATGTGGGAACAAACATCAGGAAGCCAAGTATTGAGTACAGTCTGGAAGAATTTTCGACATTGATGACTACGAACTTTGAATCAGTATTTCATCTATCCCAACTTGCTCATCCTCTTTTAAAAGCTTCTGGAAATGGAAGTATTGTGTTTGTTTCTTCGGTTGGTGGTCTCGTGAGCATTGGAAGTGGATCCATCTATGCAGCCTCTAAATCTGCAATGAATCAACTGACCAAAAATCTTGCTTGTGAATGGGCCAAGGACAATATCAGGAGCAACACTGTTGCACCCTGGTATACCAATACCCCCCTTGTAGAAAAGTTGCTTGATAATAAGACGTTCGTGGACAAGGTAGTCTCTCGAACTCCACTTGGGCGAATAGGGGAATCAAATGAAGTGTCCTCGTTGGTGGCATTCCTTTGCATGCCTGCTGCATCTTACATCACTGGACAGATTATTTCTGTTGATGGAGGGTTCACTGCCAATGGGTTTGAGTCTGGTATGAGACTAGAAAGCTTGTGAAACATGCTTCTGCTTCTTACATCACTGGGCAGACTATTTCCACAATAATCTTTTATGCTTATCATGTTTTTCTCAACTCTTTCAAGTTTTATGTAAACTCTATGTAATAATTCTAAATAATTTAATGTGCACAATCTTGAACTAAAAAAATAGTTTTAAAAGTTTTATTTTAGGGTCTTATGCACCATGGAAATATACTCTTAAAATTTATTGAGAAAA

Coding sequence (CDS)

ATGGCGGAGGCAGGCGTCAGTTCCGGAAATGCCCGATGGTCTCTCAAGGGCTTCACAGCGCTCGTCACTGGCGGCACTCGTGGAATCGGTCGCGCTGTGGTGGAGGAACTGGCCGGTTTAGGCGTGTCGGTCCACACCTGTTCCCGGAACGAACTGGAGCTCAACCAGTGCTTGAAGGAATGGGAGGGCAAAGGCTTTGTGGTCAGTGGTTCGGTTTGCGATGCTTCTTCGAGAACACAGAGAGAGAGGCTCGTACAGGAAGTGGCGTCTGCTTTCAATGGCACCCTCAATATTCTGGTAAATAATGTGGGAACAAACATCAGGAAGCCAAGTATTGAGTACAGTCTGGAAGAATTTTCGACATTGATGACTACGAACTTTGAATCAGTATTTCATCTATCCCAACTTGCTCATCCTCTTTTAAAAGCTTCTGGAAATGGAAGTATTGTGTTTGTTTCTTCGGTTGGTGGTCTCGTGAGCATTGGAAGTGGATCCATCTATGCAGCCTCTAAATCTGCAATGAATCAACTGACCAAAAATCTTGCTTGTGAATGGGCCAAGGACAATATCAGGAGCAACACTGTTGCACCCTGGTATACCAATACCCCCCTTGTAGAAAAGTTGCTTGATAATAAGACGTTCGTGGACAAGGTAGTCTCTCGAACTCCACTTGGGCGAATAGGGGAATCAAATGAAGTGTCCTCGTTGGTGGCATTCCTTTGCATGCCTGCTGCATCTTACATCACTGGACAGATTATTTCTGTTGATGGAGGGTTCACTGCCAATGGGTTTGAGTCTGGTATGAGACTAGAAAGCTTGTGA

Protein sequence

MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKEWEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFSTLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKNLACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFLCMPAASYITGQIISVDGGFTANGFESGMRLESL
Homology
BLAST of CmoCh04G012350 vs. ExPASy Swiss-Prot
Match: Q9LHT0 (Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g06060 PE=2 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 6.2e-106
Identity = 188/258 (72.87%), Postives = 217/258 (84.11%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKEWEGKGFVVSGS 71
           RWSL G TALVTGGTRGIGRAVVEELA  G  VHTCSRN+ ELN CL +W+  G VVSGS
Sbjct: 6   RWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGS 65

Query: 72  VCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFSTLMTTNFESVF 131
           VCDAS R QRE+L+QE +SAF+G LNIL+NNVGTN+RKP++EYS EE++ +M+TN ES F
Sbjct: 66  VCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAF 125

Query: 132 HLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKNLACEWAKDNIR 191
           HLSQ+AHPLLKASG GSIVF+SSV GLV + SGSIY A+K A+NQLT+NLACEWA DNIR
Sbjct: 126 HLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIR 185

Query: 192 SNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFLCMPAASYITGQ 251
           +N VAPWY  T LVE LL+ K FV+ VVSRTPLGR+GE  EVSSLVAFLC+PA+SYITGQ
Sbjct: 186 TNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQ 245

Query: 252 IISVDGGFTANGFESGMR 270
           +ISVDGGFT NGF   M+
Sbjct: 246 VISVDGGFTVNGFSYAMK 263

BLAST of CmoCh04G012350 vs. ExPASy Swiss-Prot
Match: Q9ZW12 (Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=At2g29260 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 9.3e-94
Identity = 167/253 (66.01%), Postives = 206/253 (81.42%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKEWEGKGFVVSGS 71
           RWSL G +ALVTGGTRGIGRA+VEELAGLG  VHTC+RNE EL  CL +W   GF V+GS
Sbjct: 65  RWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAGS 124

Query: 72  VCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFSTLMTTNFESVF 131
           VCD S R+QRE L++ V+S F G L+ILVNNVGTNIRKP +E++  EFSTLM+TNFESVF
Sbjct: 125 VCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESVF 184

Query: 132 HLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKNLACEWAKDNIR 191
           HL QLA+PLL+ S  GS+VF+SSV G VS+ + S+ +++K A+NQLT++LACEWAKDNIR
Sbjct: 185 HLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIR 244

Query: 192 SNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFLCMPAASYITGQ 251
            N VAPWY  T +VE++L NK ++++V S TPLGR+GE  EVSS VAFLC+PA+SYITGQ
Sbjct: 245 INAVAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQ 304

Query: 252 IISVDGGFTANGF 265
           I+ VDGG + NGF
Sbjct: 305 ILCVDGGMSINGF 317

BLAST of CmoCh04G012350 vs. ExPASy Swiss-Prot
Match: Q9ZW19 (Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 9.6e-91
Identity = 168/265 (63.40%), Postives = 206/265 (77.74%), Query Frame = 0

Query: 1   MAEAGVS-SGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLK 60
           MA+ G S     RWSL G TALVTGG++GIG AVVEELA LG  +HTC+R+E +L + L+
Sbjct: 1   MAKTGESLRDKPRWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLR 60

Query: 61  EWEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEF 120
           +W+ KGF V+ SVCD SSR +RE+L++ V++ F G LNILVNNVGT I KP+++++ E+F
Sbjct: 61  KWQAKGFQVTTSVCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDF 120

Query: 121 STLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTK 180
           S  M TN ES FHLSQLAHPLLKASG+GSIV +SSV G+V +   SIY  SK AMNQL +
Sbjct: 121 SFTMATNLESAFHLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGR 180

Query: 181 NLACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAF 240
           NLACEWA DNIR+N+V PW+  TPLV + L N+ F  +V SR P+GR+GE NEVSSLVAF
Sbjct: 181 NLACEWASDNIRTNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAF 240

Query: 241 LCMPAASYITGQIISVDGGFTANGF 265
           LC+PAASYITGQ I VDGGFT NGF
Sbjct: 241 LCLPAASYITGQTICVDGGFTVNGF 265

BLAST of CmoCh04G012350 vs. ExPASy Swiss-Prot
Match: Q9ZW18 (Senescence-associated protein 13 OS=Arabidopsis thaliana OX=3702 GN=SAG13 PE=1 SV=1)

HSP 1 Score: 333.2 bits (853), Expect = 2.8e-90
Identity = 167/264 (63.26%), Postives = 203/264 (76.89%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MA+ G    N+RWSL G TALVTGG++GIG AVVEELA LG  VHTC+R+E +L + L+E
Sbjct: 1   MAKEGGLGENSRWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLRE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           W+ KGF V+ SVCD SSR QR +L++ V+S + G LNILVNNVGT+I KP+ EY+ E+FS
Sbjct: 61  WQAKGFQVTTSVCDVSSRDQRVKLMETVSSLYQGKLNILVNNVGTSIFKPTTEYTAEDFS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
            +M TN ES FHLSQLAHPLLKASG+GSIV +SS  G+V +  GSIY A+K AMNQL +N
Sbjct: 121 FVMATNLESAFHLSQLAHPLLKASGSGSIVLISSAAGVVHVNVGSIYGATKGAMNQLARN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWA DNIR+N+V PWY  TPL     D + F  + V  TP+GR+GE+NEVS LVAFL
Sbjct: 181 LACEWASDNIRTNSVCPWYITTPLSNDFFDEE-FKKEAVRTTPMGRVGEANEVSPLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGF 265
           C+P+ASYITGQ I VDGG T NGF
Sbjct: 241 CLPSASYITGQTICVDGGATVNGF 263

BLAST of CmoCh04G012350 vs. ExPASy Swiss-Prot
Match: Q9ZW13 (Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g29290 PE=2 SV=1)

HSP 1 Score: 331.6 bits (849), Expect = 8.1e-90
Identity = 161/253 (63.64%), Postives = 198/253 (78.26%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKEWEGKGFVVSGS 71
           RWSL+G  ALVTGGT+GIG AVVEEL+ LG  VHTC+R+E +L + L+EW+ KGF V+ S
Sbjct: 4   RWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTS 63

Query: 72  VCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFSTLMTTNFESVF 131
           +CD S R QRE+L++ V+S F G LNILVNNVGT + KP+ EY+ EEFS LM TN +S F
Sbjct: 64  ICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAF 123

Query: 132 HLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKNLACEWAKDNIR 191
           H+SQLAHPLLKASG+GSIV +SS+ G+V +G GSIY A+K AMNQL +NLACEWA DNIR
Sbjct: 124 HISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIR 183

Query: 192 SNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFLCMPAASYITGQ 251
           +N + PW   TPL+  LL  +    +   RTP+GR+GE+NEVS LVAFLC+PAASYITGQ
Sbjct: 184 TNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQ 243

Query: 252 IISVDGGFTANGF 265
           +I VDGG T NGF
Sbjct: 244 VICVDGGLTVNGF 256

BLAST of CmoCh04G012350 vs. ExPASy TrEMBL
Match: A0A6J1GWZ5 (tropinone reductase homolog At5g06060 OS=Cucurbita moschata OX=3662 GN=LOC111458319 PE=4 SV=1)

HSP 1 Score: 530.8 bits (1366), Expect = 3.4e-147
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE
Sbjct: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS
Sbjct: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           CMPAASYITGQIISVDGGFTANGFESGMRLESL
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 273

BLAST of CmoCh04G012350 vs. ExPASy TrEMBL
Match: A0A6J1IKI1 (tropinone reductase homolog At5g06060 OS=Cucurbita maxima OX=3661 GN=LOC111477082 PE=4 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 7.8e-144
Identity = 266/273 (97.44%), Postives = 270/273 (98.90%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEAGVSSGN+RWSLKGFTALVTGGTRGIGRAVVEELAGLG SVHTCSRNELELNQCLKE
Sbjct: 1   MAEAGVSSGNSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WEGKGFVVSGSVCDASSRTQRE+LVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEE+S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQREKLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEYS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIRSNTVAPWYTNT LVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTSLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           CMPAASYITGQIISVDGGFTANGFE GMRL+SL
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFEPGMRLDSL 273

BLAST of CmoCh04G012350 vs. ExPASy TrEMBL
Match: A0A6J1CCC2 (tropinone reductase homolog At5g06060 OS=Momordica charantia OX=3673 GN=LOC111010272 PE=4 SV=1)

HSP 1 Score: 483.4 bits (1243), Expect = 6.2e-133
Identity = 244/273 (89.38%), Postives = 258/273 (94.51%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEAG  SGN+RWSLKG TALVTGGTRGIGRAVVEELA LG SVHTCSRNE ELN+CLKE
Sbjct: 1   MAEAGDGSGNSRWSLKGSTALVTGGTRGIGRAVVEELAALGASVHTCSRNEAELNRCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WEGKGFVV GSVCDASSRT RERL+QEV SAFNG LNILVNNVG NIRKP+IEYSLEEFS
Sbjct: 61  WEGKGFVVRGSVCDASSRTHRERLMQEVESAFNGRLNILVNNVGKNIRKPTIEYSLEEFS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           +LMTTNFES +HLSQL+HPLLKASGNGSIVF+SSVGGL+SIGSGSIYAA+KSA+NQLTKN
Sbjct: 121 SLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLMSIGSGSIYAATKSAINQLTKN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIRSN VAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNCVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           C+P ASYITGQIISVDGGFTANGF+SGMRL+ L
Sbjct: 241 CLPTASYITGQIISVDGGFTANGFDSGMRLDML 273

BLAST of CmoCh04G012350 vs. ExPASy TrEMBL
Match: A0A0A0KS92 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G184270 PE=4 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 2.1e-128
Identity = 234/273 (85.71%), Postives = 253/273 (92.67%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEA  S+GN+RWSL+GFTALVTGGTRGIG AVVEELAGLG SVHTC+RNE +LNQCLKE
Sbjct: 1   MAEASGSAGNSRWSLEGFTALVTGGTRGIGHAVVEELAGLGASVHTCARNESDLNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WE KG+VV+GSVCDASSRTQRE L+QEVAS+FNGTLNILVNNVGTNIRKPS EYSLEE S
Sbjct: 61  WEAKGYVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNVGTNIRKPSAEYSLEEVS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           TLMTTNFES FHLSQL+HPLLKASGNGSIVF+SSVGGLVSIGSGSIYAA+KSA+NQLT+N
Sbjct: 121 TLMTTNFESAFHLSQLSHPLLKASGNGSIVFISSVGGLVSIGSGSIYAATKSAINQLTRN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           L CEWAKDNIR N VAPWY NTPLVEKL+ NKT VD +VSRTPLGRIGES EVSSLVAFL
Sbjct: 181 LTCEWAKDNIRVNCVAPWYINTPLVEKLMKNKTLVDNIVSRTPLGRIGESKEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           C+PAASYITGQI+SVDGGFTANGFE GMRL+ L
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of CmoCh04G012350 vs. ExPASy TrEMBL
Match: A0A5D3BV45 (Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold293G00720 PE=4 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 1.2e-125
Identity = 231/273 (84.62%), Postives = 250/273 (91.58%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEA  S+G  RWSLKGFTALVTGGTRGIG AVVEELAGLG SVHTCSRN+ +LNQCLKE
Sbjct: 1   MAEASDSAGIPRWSLKGFTALVTGGTRGIGHAVVEELAGLGASVHTCSRNQSDLNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WE KGFVV+GSVCDASSRTQRE L+QEVAS+FNGTLNILVNN GTNIRKP+ EYSLE+ S
Sbjct: 61  WEAKGFVVTGSVCDASSRTQREELIQEVASSFNGTLNILVNNAGTNIRKPTAEYSLEDVS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           TLMTTNFES +HLSQL+HPLLKASGNGSIVF+SSVGGLVS+GSGSIYAA+KSA+NQLTK 
Sbjct: 121 TLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLVSVGSGSIYAATKSAINQLTKY 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIR N VAPWY NTPLVEKLL+NKT VD +VSRTPL RIGES EVSSLVAFL
Sbjct: 181 LACEWAKDNIRVNGVAPWYINTPLVEKLLNNKTLVDNIVSRTPLRRIGESKEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           C+PAASYITGQI+SVDGGFTANGFE GMRL+ L
Sbjct: 241 CLPAASYITGQIMSVDGGFTANGFEPGMRLDKL 273

BLAST of CmoCh04G012350 vs. NCBI nr
Match: XP_022956657.1 (tropinone reductase homolog At5g06060 [Cucurbita moschata])

HSP 1 Score: 530.8 bits (1366), Expect = 7.0e-147
Identity = 273/273 (100.00%), Postives = 273/273 (100.00%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE
Sbjct: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS
Sbjct: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           CMPAASYITGQIISVDGGFTANGFESGMRLESL
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 273

BLAST of CmoCh04G012350 vs. NCBI nr
Match: XP_023547195.1 (tropinone reductase homolog At5g06060 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 525.8 bits (1353), Expect = 2.2e-145
Identity = 270/273 (98.90%), Postives = 271/273 (99.27%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLG SVHTCSRNELELNQCLKE
Sbjct: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS
Sbjct: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
            LMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN
Sbjct: 121 ILMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           CMPAASYITGQIISVDGGFTANGFESGMRL+SL
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFESGMRLDSL 273

BLAST of CmoCh04G012350 vs. NCBI nr
Match: KAG6600980.1 (Tropinone reductase-like protein, partial [Cucurbita argyrosperma subsp. sororia] >KAG7031592.1 Tropinone reductase-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 525.4 bits (1352), Expect = 2.9e-145
Identity = 269/273 (98.53%), Postives = 271/273 (99.27%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLG SVHTCSRNELELNQCLKE
Sbjct: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WEGKGFVV GSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS
Sbjct: 61  WEGKGFVVGGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           TLMTTNFESVFHLSQL+HPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN
Sbjct: 121 TLMTTNFESVFHLSQLSHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           CMPAASYITGQIISVDGGFTANGFESGMRL+SL
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFESGMRLDSL 273

BLAST of CmoCh04G012350 vs. NCBI nr
Match: XP_022976820.1 (tropinone reductase homolog At5g06060 [Cucurbita maxima])

HSP 1 Score: 519.6 bits (1337), Expect = 1.6e-143
Identity = 266/273 (97.44%), Postives = 270/273 (98.90%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEAGVSSGN+RWSLKGFTALVTGGTRGIGRAVVEELAGLG SVHTCSRNELELNQCLKE
Sbjct: 1   MAEAGVSSGNSRWSLKGFTALVTGGTRGIGRAVVEELAGLGASVHTCSRNELELNQCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WEGKGFVVSGSVCDASSRTQRE+LVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEE+S
Sbjct: 61  WEGKGFVVSGSVCDASSRTQREKLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEYS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN
Sbjct: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIRSNTVAPWYTNT LVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNTVAPWYTNTSLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           CMPAASYITGQIISVDGGFTANGFE GMRL+SL
Sbjct: 241 CMPAASYITGQIISVDGGFTANGFEPGMRLDSL 273

BLAST of CmoCh04G012350 vs. NCBI nr
Match: XP_022139331.1 (tropinone reductase homolog At5g06060 [Momordica charantia])

HSP 1 Score: 483.4 bits (1243), Expect = 1.3e-132
Identity = 244/273 (89.38%), Postives = 258/273 (94.51%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MAEAG  SGN+RWSLKG TALVTGGTRGIGRAVVEELA LG SVHTCSRNE ELN+CLKE
Sbjct: 1   MAEAGDGSGNSRWSLKGSTALVTGGTRGIGRAVVEELAALGASVHTCSRNEAELNRCLKE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           WEGKGFVV GSVCDASSRT RERL+QEV SAFNG LNILVNNVG NIRKP+IEYSLEEFS
Sbjct: 61  WEGKGFVVRGSVCDASSRTHRERLMQEVESAFNGRLNILVNNVGKNIRKPTIEYSLEEFS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
           +LMTTNFES +HLSQL+HPLLKASGNGSIVF+SSVGGL+SIGSGSIYAA+KSA+NQLTKN
Sbjct: 121 SLMTTNFESAYHLSQLSHPLLKASGNGSIVFISSVGGLMSIGSGSIYAATKSAINQLTKN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWAKDNIRSN VAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL
Sbjct: 181 LACEWAKDNIRSNCVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGFESGMRLESL 274
           C+P ASYITGQIISVDGGFTANGF+SGMRL+ L
Sbjct: 241 CLPTASYITGQIISVDGGFTANGFDSGMRLDML 273

BLAST of CmoCh04G012350 vs. TAIR 10
Match: AT5G06060.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 385.2 bits (988), Expect = 4.4e-107
Identity = 188/258 (72.87%), Postives = 217/258 (84.11%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKEWEGKGFVVSGS 71
           RWSL G TALVTGGTRGIGRAVVEELA  G  VHTCSRN+ ELN CL +W+  G VVSGS
Sbjct: 6   RWSLAGKTALVTGGTRGIGRAVVEELAKFGAKVHTCSRNQEELNACLNDWKANGLVVSGS 65

Query: 72  VCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFSTLMTTNFESVF 131
           VCDAS R QRE+L+QE +SAF+G LNIL+NNVGTN+RKP++EYS EE++ +M+TN ES F
Sbjct: 66  VCDASVRDQREKLIQEASSAFSGKLNILINNVGTNVRKPTVEYSSEEYAKIMSTNLESAF 125

Query: 132 HLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKNLACEWAKDNIR 191
           HLSQ+AHPLLKASG GSIVF+SSV GLV + SGSIY A+K A+NQLT+NLACEWA DNIR
Sbjct: 126 HLSQIAHPLLKASGVGSIVFISSVAGLVHLSSGSIYGATKGALNQLTRNLACEWASDNIR 185

Query: 192 SNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFLCMPAASYITGQ 251
           +N VAPWY  T LVE LL+ K FV+ VVSRTPLGR+GE  EVSSLVAFLC+PA+SYITGQ
Sbjct: 186 TNCVAPWYIKTSLVETLLEKKEFVEAVVSRTPLGRVGEPEEVSSLVAFLCLPASSYITGQ 245

Query: 252 IISVDGGFTANGFESGMR 270
           +ISVDGGFT NGF   M+
Sbjct: 246 VISVDGGFTVNGFSYAMK 263

BLAST of CmoCh04G012350 vs. TAIR 10
Match: AT2G29260.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 344.7 bits (883), Expect = 6.6e-95
Identity = 167/253 (66.01%), Postives = 206/253 (81.42%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKEWEGKGFVVSGS 71
           RWSL G +ALVTGGTRGIGRA+VEELAGLG  VHTC+RNE EL  CL +W   GF V+GS
Sbjct: 65  RWSLNGMSALVTGGTRGIGRAIVEELAGLGAEVHTCARNEYELENCLSDWNRSGFRVAGS 124

Query: 72  VCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFSTLMTTNFESVF 131
           VCD S R+QRE L++ V+S F G L+ILVNNVGTNIRKP +E++  EFSTLM+TNFESVF
Sbjct: 125 VCDVSDRSQREALMETVSSVFEGKLHILVNNVGTNIRKPMVEFTAGEFSTLMSTNFESVF 184

Query: 132 HLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKNLACEWAKDNIR 191
           HL QLA+PLL+ S  GS+VF+SSV G VS+ + S+ +++K A+NQLT++LACEWAKDNIR
Sbjct: 185 HLCQLAYPLLRESKAGSVVFISSVSGFVSLKNMSVQSSTKGAINQLTRSLACEWAKDNIR 244

Query: 192 SNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFLCMPAASYITGQ 251
            N VAPWY  T +VE++L NK ++++V S TPLGR+GE  EVSS VAFLC+PA+SYITGQ
Sbjct: 245 INAVAPWYIKTSMVEQVLSNKEYLEEVYSVTPLGRLGEPREVSSAVAFLCLPASSYITGQ 304

Query: 252 IISVDGGFTANGF 265
           I+ VDGG + NGF
Sbjct: 305 ILCVDGGMSINGF 317

BLAST of CmoCh04G012350 vs. TAIR 10
Match: AT2G29360.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 334.7 bits (857), Expect = 6.8e-92
Identity = 168/265 (63.40%), Postives = 206/265 (77.74%), Query Frame = 0

Query: 1   MAEAGVS-SGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLK 60
           MA+ G S     RWSL G TALVTGG++GIG AVVEELA LG  +HTC+R+E +L + L+
Sbjct: 1   MAKTGESLRDKPRWSLVGMTALVTGGSKGIGEAVVEELATLGARIHTCARDETQLQESLR 60

Query: 61  EWEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEF 120
           +W+ KGF V+ SVCD SSR +RE+L++ V++ F G LNILVNNVGT I KP+++++ E+F
Sbjct: 61  KWQAKGFQVTTSVCDVSSRDKREKLMETVSTIFEGKLNILVNNVGTCIVKPTLQHTAEDF 120

Query: 121 STLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTK 180
           S  M TN ES FHLSQLAHPLLKASG+GSIV +SSV G+V +   SIY  SK AMNQL +
Sbjct: 121 SFTMATNLESAFHLSQLAHPLLKASGSGSIVLISSVSGVVHVNGASIYGVSKGAMNQLGR 180

Query: 181 NLACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAF 240
           NLACEWA DNIR+N+V PW+  TPLV + L N+ F  +V SR P+GR+GE NEVSSLVAF
Sbjct: 181 NLACEWASDNIRTNSVCPWFIETPLVTESLSNEEFRKEVESRPPMGRVGEVNEVSSLVAF 240

Query: 241 LCMPAASYITGQIISVDGGFTANGF 265
           LC+PAASYITGQ I VDGGFT NGF
Sbjct: 241 LCLPAASYITGQTICVDGGFTVNGF 265

BLAST of CmoCh04G012350 vs. TAIR 10
Match: AT2G29350.1 (senescence-associated gene 13 )

HSP 1 Score: 333.2 bits (853), Expect = 2.0e-91
Identity = 167/264 (63.26%), Postives = 203/264 (76.89%), Query Frame = 0

Query: 1   MAEAGVSSGNARWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKE 60
           MA+ G    N+RWSL G TALVTGG++GIG AVVEELA LG  VHTC+R+E +L + L+E
Sbjct: 1   MAKEGGLGENSRWSLGGMTALVTGGSKGIGEAVVEELAMLGAKVHTCARDETQLQERLRE 60

Query: 61  WEGKGFVVSGSVCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFS 120
           W+ KGF V+ SVCD SSR QR +L++ V+S + G LNILVNNVGT+I KP+ EY+ E+FS
Sbjct: 61  WQAKGFQVTTSVCDVSSRDQRVKLMETVSSLYQGKLNILVNNVGTSIFKPTTEYTAEDFS 120

Query: 121 TLMTTNFESVFHLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKN 180
            +M TN ES FHLSQLAHPLLKASG+GSIV +SS  G+V +  GSIY A+K AMNQL +N
Sbjct: 121 FVMATNLESAFHLSQLAHPLLKASGSGSIVLISSAAGVVHVNVGSIYGATKGAMNQLARN 180

Query: 181 LACEWAKDNIRSNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFL 240
           LACEWA DNIR+N+V PWY  TPL     D + F  + V  TP+GR+GE+NEVS LVAFL
Sbjct: 181 LACEWASDNIRTNSVCPWYITTPLSNDFFDEE-FKKEAVRTTPMGRVGEANEVSPLVAFL 240

Query: 241 CMPAASYITGQIISVDGGFTANGF 265
           C+P+ASYITGQ I VDGG T NGF
Sbjct: 241 CLPSASYITGQTICVDGGATVNGF 263

BLAST of CmoCh04G012350 vs. TAIR 10
Match: AT2G29290.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 331.6 bits (849), Expect = 5.8e-91
Identity = 161/253 (63.64%), Postives = 198/253 (78.26%), Query Frame = 0

Query: 12  RWSLKGFTALVTGGTRGIGRAVVEELAGLGVSVHTCSRNELELNQCLKEWEGKGFVVSGS 71
           RWSL+G  ALVTGGT+GIG AVVEEL+ LG  VHTC+R+E +L + L+EW+ KGF V+ S
Sbjct: 4   RWSLQGMNALVTGGTKGIGEAVVEELSILGARVHTCARDETQLQERLREWQEKGFQVTTS 63

Query: 72  VCDASSRTQRERLVQEVASAFNGTLNILVNNVGTNIRKPSIEYSLEEFSTLMTTNFESVF 131
           +CD S R QRE+L++ V+S F G LNILVNNVGT + KP+ EY+ EEFS LM TN +S F
Sbjct: 64  ICDVSLREQREKLMETVSSLFQGKLNILVNNVGTLMLKPTTEYTAEEFSFLMATNLDSAF 123

Query: 132 HLSQLAHPLLKASGNGSIVFVSSVGGLVSIGSGSIYAASKSAMNQLTKNLACEWAKDNIR 191
           H+SQLAHPLLKASG+GSIV +SS+ G+V +G GSIY A+K AMNQL +NLACEWA DNIR
Sbjct: 124 HISQLAHPLLKASGSGSIVLMSSIAGVVHVGVGSIYGATKGAMNQLARNLACEWASDNIR 183

Query: 192 SNTVAPWYTNTPLVEKLLDNKTFVDKVVSRTPLGRIGESNEVSSLVAFLCMPAASYITGQ 251
           +N + PW   TPL+  LL  +    +   RTP+GR+GE+NEVS LVAFLC+PAASYITGQ
Sbjct: 184 TNAICPWLITTPLISDLLSVEEMKKEAEERTPMGRVGEANEVSPLVAFLCLPAASYITGQ 243

Query: 252 IISVDGGFTANGF 265
           +I VDGG T NGF
Sbjct: 244 VICVDGGLTVNGF 256

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LHT06.2e-10672.87Tropinone reductase homolog At5g06060 OS=Arabidopsis thaliana OX=3702 GN=At5g060... [more]
Q9ZW129.3e-9466.01Tropinone reductase homolog At2g29260, chloroplastic OS=Arabidopsis thaliana OX=... [more]
Q9ZW199.6e-9163.40Tropinone reductase homolog At2g29360 OS=Arabidopsis thaliana OX=3702 GN=SDR PE=... [more]
Q9ZW182.8e-9063.26Senescence-associated protein 13 OS=Arabidopsis thaliana OX=3702 GN=SAG13 PE=1 S... [more]
Q9ZW138.1e-9063.64Tropinone reductase homolog At2g29290 OS=Arabidopsis thaliana OX=3702 GN=At2g292... [more]
Match NameE-valueIdentityDescription
A0A6J1GWZ53.4e-147100.00tropinone reductase homolog At5g06060 OS=Cucurbita moschata OX=3662 GN=LOC111458... [more]
A0A6J1IKI17.8e-14497.44tropinone reductase homolog At5g06060 OS=Cucurbita maxima OX=3661 GN=LOC11147708... [more]
A0A6J1CCC26.2e-13389.38tropinone reductase homolog At5g06060 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A0A0KS922.1e-12885.71Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G184270 PE=4 SV=1[more]
A0A5D3BV451.2e-12584.62Tropinone reductase-like protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676... [more]
Match NameE-valueIdentityDescription
XP_022956657.17.0e-147100.00tropinone reductase homolog At5g06060 [Cucurbita moschata][more]
XP_023547195.12.2e-14598.90tropinone reductase homolog At5g06060 [Cucurbita pepo subsp. pepo][more]
KAG6600980.12.9e-14598.53Tropinone reductase-like protein, partial [Cucurbita argyrosperma subsp. sororia... [more]
XP_022976820.11.6e-14397.44tropinone reductase homolog At5g06060 [Cucurbita maxima][more]
XP_022139331.11.3e-13289.38tropinone reductase homolog At5g06060 [Momordica charantia][more]
Match NameE-valueIdentityDescription
AT5G06060.14.4e-10772.87NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29260.16.6e-9566.01NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29360.16.8e-9263.40NAD(P)-binding Rossmann-fold superfamily protein [more]
AT2G29350.12.0e-9163.26senescence-associated gene 13 [more]
AT2G29290.25.8e-9163.64NAD(P)-binding Rossmann-fold superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita moschata (Rifu) v1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00080SDRFAMILYcoord: 167..186
score: 38.79
coord: 94..105
score: 45.16
coord: 147..155
score: 49.39
IPR002347Short-chain dehydrogenase/reductase SDRPRINTSPR00081GDHRDHcoord: 94..105
score: 49.49
coord: 223..243
score: 33.16
coord: 141..157
score: 34.22
coord: 19..36
score: 50.14
coord: 188..205
score: 35.79
coord: 167..186
score: 42.82
NoneNo IPR availablePFAMPF13561adh_short_C2coord: 26..260
e-value: 2.1E-58
score: 197.7
NoneNo IPR availableGENE3D3.40.50.720coord: 8..263
e-value: 5.1E-81
score: 273.6
NoneNo IPR availablePIRSRPIRSR000095-1PIRSR000095-1coord: 21..212
e-value: 5.3E-17
score: 59.8
NoneNo IPR availablePANTHERPTHR42898:SF69NAD(P)-BINDING ROSSMANN-FOLD PROTEINcoord: 7..267
IPR045000Tropinone reductasePANTHERPTHR42898TROPINONE REDUCTASEcoord: 7..267
IPR020904Short-chain dehydrogenase/reductase, conserved sitePROSITEPS00061ADH_SHORTcoord: 154..182
IPR036291NAD(P)-binding domain superfamilySUPERFAMILY51735NAD(P)-binding Rossmann-fold domainscoord: 17..262

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmoCh04G012350.1CmoCh04G012350.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016491 oxidoreductase activity