Cmc01g0004571 (gene) Melon (Charmono) v1.1

Overview
NameCmc01g0004571
Typegene
OrganismCucumis melo L. var. cantalupensis cv. Charmono (Melon (Charmono) v1.1)
DescriptionSqualene monooxygenase
LocationCMiso1.1chr01: 1089358 .. 1094414 (-)
RNA-Seq ExpressionCmc01g0004571
SyntenyCmc01g0004571
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAAAAGACCTAAAAATCCTTCATCTCTCTCTCACATTCCTTTCTCAAACAATTCATCTCCGTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCATCTCCAAGACACAAATGGAGCTTCTATCGGCGGCTCTGTTTGGCCTTCTCTTGGCTTCCATCCTTCCTCTCATTTTCTTCTTCGCTTTCAAAGATGGAATCAACAGCTCCTCCCCTCCCAATCCCATCCATTCTAAATCTATCAATTCCCCCTCCGCCGCCGCCCTACAGATTCCCCCCCACTCCGATGACGACCACCCTGACCTCATCGTCGTCGGCGCTGGTGTCGCCGGCGCCGCTCTTGCTTTTACTCTCGCCAAGGTCCGTCGTTTATCTCTCCCGCATTTCCTTCTCTTCGTCTTTAATTTTTTGAATTTCTTTGTTATTATATTAAATTTATTTGAATATATTAACATTTTATTTGTTTTTTAGTATTATATATATATATATATATATATATTTAAATTAATTTTATGATTTATTAAAATTGTGTATGCTAAATAAATAAAAATTTAAATGTTTAAATTATTATTTTGTAGGAAATAAGACCTCAATTTCTTGTCATATAATTTTACTTTTACTCTAAACATATTTATTTAGTTTTTATTTTGTCTCTAAATAAATTAGTACTTTTATTTTAATTACTTTCAATTTACTTTACTAAAATACTTAACTTATAAGGCTCCCTATTTAACATAATTTAATACAAAATTAATGAAAATTTTAATACTCTAAGAATTAAAATTAAAAAAATTTCAAACTAAAATTAAACAAATATAATTCTTCCTGAAAAAAAAATTAAAATTAAGAAAAATAGGACCACTTGAATCTAATGATCTTTTTATTTATTTATTTTTTGGAAGACTGTAATGAGAACTTTGCCTAAAAAACAAATAATAATTTCCAATAATGATAAATAAATTCCACTACTAATCTACTACCCATCTAACAACCAATTTGTGACATTAGAAAAAGAAATAGGGTTCTAACTATTGAAACATAACATCAATGTTACATCACATCAATCTAATTTTTTTTAAAAAAACCAATACATTCAACTTACAATTTTAAATTTCTTTTGTGAAATAATTTAGTTCATACCATATTATATTCATTAAGGTAGAATAACAAATTTGCCCAAAATCTATGATTTTATTTAAATATTACGTGTATATATTTTGGCTATTCAAACAGAAAAAAGGGAAGCGGCAGAATTTGTATATATATATATATGATTTAGGTGTTTGATTTTTTTTTTTTAATTTCAATTTTGAGTATATGTAGCTGTCAAATTTTTGAATATATAAAAAATATTTTTGGCGTAAGTGATAAGTTTAATTAAATTAATTAATTAAGTAAGGATTATGGAAATTGACTTTTCCTTTGATATATTCAAATAATTAGAACTTGTGTTTTTAATTATTAATTATAAGAGGAGGGAGTTGTTGGCATGATTCATTTATTTATTTTAATTTCTTTTTATTGATGAAAAATAATTAGAATCAATAAGGAATGAACTAAAAAGTTAAATTGTATAGTTTAATTTCTCATTTAAATATAGAAGAATGGGGAAGAAAATATTTTGATACCCCAATTATTAATTTGGCTTGGTCTTATCTCTATAGTCTTTCCTTAATTTTTCATTTTTTATTAATAAAATTCAATTTTAATATCAAATTATAACATAATTTCAAAGTCCAATATAACTTGATTTCTATGGTATAAGTTAGAAGTTTTTTAACTTTTTAATAAATCTGGAGAGTTGATTTTTGTTAATTTGTTTTTAGAAAATATTAGTCAATATTAACATTCCGTTTATAAATGGTGAAAATAAATTCGTATAATCTTATTATTCTTCGATGAATTATATCTTTTAAATAATTTTGAAGAACGATAGAGATTAAAATTAGACCATTGGAAATACGAAAAATAGAATAGAGCAAAATAAATACAATTACGAAAAAGGTTAAAAGTATAGTAATTAAATTTATTAATAAATAAAATAATATTTTAAAAAATTATAAAAATAGTAAAATTTATCGAAGTGTATTATTAATAGAAGATTAGAATTCTATAGATTAATTAATTTATTTCTTTATTGACAACGGAGAGTCAAACGTTTTGACTTAGAGATTGACCTCTGGCTGAGAGATTTGACTTTGAGAATGTAGGATGGAAGAAAAGTGCACGTAATCGAGAGGGACTTGACGGAGCCAGATCGAATCGTCGGAGAATTGCTTCAACCCGGCGGCTATTTGAAGCTCATCGAGTTGGGGCTTGAAGGTAACCCCCAAATTCCCATTTCTCATTTCTTCCAATTCCTCAATCTTCCCTTTTATTCCTAAAACTACACTTCTAATCGCTTCCTTCTTCTGCTTTTTGAATCCATTTCCCACAGACAGTCTGAACGGAATCGATGCTCAGCAGGTGTTTGGTTATGCCCTCTACAAGAATGGCAGTCACACCAGATTGGCTTATCCTTTGGAGAAATTTGATTCCAATGTTTCTGGAAGAAGCTTTCACAATGGCCGTTTCATTCAGAGATTGAGGGAAAAGGCTGCTGCTCTTTCAAAGTAACTTACTCAATTTCCCCTCTTCTTTCATGTTTTTCTTCTTGCTATACCCCGTTTTTTGTTTTGGTGTTGTTTTGTTTTTTTGTTCTTGATCTTTTCAACTGACAGTTGTAGTTTCTGGGATTTTAGAAAGTTTTCATTTTAGTCCCTTTTTATTATTTTTTTAATTTTATCCCCTTAAGCTGGTATGTTATTTTACATTTTTTTCATTGATTTAGAATTGGTTTGAAGCATGTTTCGGAGTGATTTTGAACATAGCAAAACTGGGTTTGGTTGTTCTGACCATTTCAAAGATTTATGAACATGCCATTTGCCAATTTCTATATGTCCATGCTAAAGCTACATTATCCATTTGTAAGAAACGGAATACAGAAAAGAACAAAACTGAAAGTTCAGACTAACATTAGATGTTTTAAAAGCTTAGGGAGAAAATTGAATATTAAGTTTCCCAATTCACTTGTGTTTTAGGAAAGCTGATGTTTTTGTTATTACATAAATCATGGGCTCCTGTATGTTACTTGAAATGATCGTTAGTTCTTGGATTTGATTTTAGGAGCAACGTCATATAACAAAACAACTGCTTTGATTTTCGACATTTTTCTTCCTTTCCATTTCACTGATTCTTGGTTTATATCTGTTATTCAATTTTGATATCTTTCCAGTGTGAGAATGGAACAAGGGACTGTAACGTCCATAGTTGAAGAAGATGGGATTGTGAAAGGTGTGCAATATAAAACCAAGAACGGCAAAGAGTTGACAGCTTATGCTCCCCTCACAATTGTTTGTGATGGTGGCTTTTCGAATTTGCGTCGCAAGCTCTGCAAACCTCAGGTAATTCGTATGGAATGTATTGAAGAAGTATTCAAGTTCTGATCGTTGATCAGTATATGCAAGTGATATTACAAGCAGTCATTTTATAGAAGTTCGGATTACTTGATGCTTAAGCCCCTGATGATGTTCTCTGAAAACCTTGGTCATGATCCTTCCTGCAGATTGACATTCCCTCATGCTTTGTTGGTTTGGTCCTGGAGAACTGCAAGCTTCCCCTTGAAAATCATGGACATGTTGTGCTAGCAGATCCATCACCTATCTTGTTATACCCGATTAGCAGCACAGAGGTACGCTGCTTAGTCGATATCCCCGGCCAAAAAGTCCCTTCTGTTGCTAATGGTGAAATGGCACACTACTTGAAAACTGCTGTAGCTCCTCAGGTACCTTTACATATTGGTTTCTGAACTTTGTTCTGTATGTTCTTTTTTCATTGGCACCTAGAAGTGCAAAAATCTGCCCTTTCGGCTCGATGTTGATTTTGAGAATTTCATATTCCAGCTACCGCCTGAGGTGTATCCTGCCTTCATTGCTGCAATTGATAAAGGAAACATAAAAAGCACAACAAATAGAAGTATGCCAGCAGCACCACAATCCACGCCAGGAGCACTTCTCTTGGGTGATGCATTCAACATGCGACATCCTTTAACAGGTGGAGGAATGACGGTTGCTCTCTCTGATATCGTTGTGCTACGAGACCTTCTTCGACCCCTTTCTAATTTAAACGATGCTGATGTCTTGTGCAACTATCTCGAATCCTTCTACACTTTGCGCAAGGTATCACATACATGCTAATAGAGCCACCTCGTTATACTAGTTTTGGCCTACCACCCTCACCTCGAGCTTTCTTAAACTAACAGTGGCTTTCATTCATCCCTTGCAGCCAGTGGCATCTACAATAAACACATTAGCAGGTGCCTTATACAAGGTGTTTTGTGCATCACCAGATCCAGCCAGGAAGGAAATGCGCGAAGCTTGTTTCGATTATTTGAGTTTAGGAGGCGTGTTTTCATCAGGACCAGTGGCCCTTCTCTCTGGTCTTAACCCACGCCCATTAAGCTTGTTCTGTCACTTCTTTGCTGTTGCTATATACGGTGTTGGTCGGTTGCTAATCCCATTCCCCTCACCTAAGCGTATATGGATTGGAGTTCGGTTGATTACGGTACACACATCTATCATCATCCAAATCCTGCTTACATCTGCATATGAACTTACATTATCGATTGAAAGGAGCATTTTGCTAAATTATATCTAAACTTTGAATGTTGTAGGGAGCAGCGGGCATAATTTTTCCCATAATAAAGGATGAAGGAGTGAGACAAATGTTCTTCCCCGCAACAGTGCCTGCATATTACAGAACACCTCCTATGTTGTGAAAGCAAAGGAACAAAGAAAAGAAAAGAAAAGAAAAAAATGAAAATCACACAAAAAATGAAAAAAGAGAATTTCTGTGAAGAAAACCTTATTGTATTTAGTAGTGTATCTCCCAAAGTCCAAAGAATTGAAGGAGGAAAGGCTACTTATAGGAAAATGAACTTAAATGTAGAGTTTATCTGTTGAGCTTTATTTTATTATTATTATTATTAATTATTATTATTTTCATCTTCTTTCTTTCA

mRNA sequence

AAAAAGACCTAAAAATCCTTCATCTCTCTCTCACATTCCTTTCTCAAACAATTCATCTCCGTCTCCCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCTCCATCTCCAAGACACAAATGGAGCTTCTATCGGCGGCTCTGTTTGGCCTTCTCTTGGCTTCCATCCTTCCTCTCATTTTCTTCTTCGCTTTCAAAGATGGAATCAACAGCTCCTCCCCTCCCAATCCCATCCATTCTAAATCTATCAATTCCCCCTCCGCCGCCGCCCTACAGATTCCCCCCCACTCCGATGACGACCACCCTGACCTCATCGTCGTCGGCGCTGGTGTCGCCGGCGCCGCTCTTGCTTTTACTCTCGCCAAGGATGGAAGAAAAGTGCACGTAATCGAGAGGGACTTGACGGAGCCAGATCGAATCGTCGGAGAATTGCTTCAACCCGGCGGCTATTTGAAGCTCATCGAGTTGGGGCTTGAAGACAGTCTGAACGGAATCGATGCTCAGCAGGTGTTTGGTTATGCCCTCTACAAGAATGGCAGTCACACCAGATTGGCTTATCCTTTGGAGAAATTTGATTCCAATGTTTCTGGAAGAAGCTTTCACAATGGCCGTTTCATTCAGAGATTGAGGGAAAAGGCTGCTGCTCTTTCAAATGTGAGAATGGAACAAGGGACTGTAACGTCCATAGTTGAAGAAGATGGGATTGTGAAAGGTGTGCAATATAAAACCAAGAACGGCAAAGAGTTGACAGCTTATGCTCCCCTCACAATTGTTTGTGATGGTGGCTTTTCGAATTTGCGTCGCAAGCTCTGCAAACCTCAGATTGACATTCCCTCATGCTTTGTTGGTTTGGTCCTGGAGAACTGCAAGCTTCCCCTTGAAAATCATGGACATGTTGTGCTAGCAGATCCATCACCTATCTTGTTATACCCGATTAGCAGCACAGAGGTACGCTGCTTAGTCGATATCCCCGGCCAAAAAGTCCCTTCTGTTGCTAATGGTGAAATGGCACACTACTTGAAAACTGCTGTAGCTCCTCAGCTACCGCCTGAGGTGTATCCTGCCTTCATTGCTGCAATTGATAAAGGAAACATAAAAAGCACAACAAATAGAAGTATGCCAGCAGCACCACAATCCACGCCAGGAGCACTTCTCTTGGGTGATGCATTCAACATGCGACATCCTTTAACAGGTGGAGGAATGACGGTTGCTCTCTCTGATATCGTTGTGCTACGAGACCTTCTTCGACCCCTTTCTAATTTAAACGATGCTGATGTCTTGTGCAACTATCTCGAATCCTTCTACACTTTGCGCAAGCCAGTGGCATCTACAATAAACACATTAGCAGGTGCCTTATACAAGGTGTTTTGTGCATCACCAGATCCAGCCAGGAAGGAAATGCGCGAAGCTTGTTTCGATTATTTGAGTTTAGGAGGCGTGTTTTCATCAGGACCAGTGGCCCTTCTCTCTGGTCTTAACCCACGCCCATTAAGCTTGTTCTGTCACTTCTTTGCTGTTGCTATATACGGTGTTGGTCGGTTGCTAATCCCATTCCCCTCACCTAAGCGTATATGGATTGGAGTTCGGTTGATTACGGGAGCAGCGGGCATAATTTTTCCCATAATAAAGGATGAAGGAGTGAGACAAATGTTCTTCCCCGCAACAGTGCCTGCATATTACAGAACACCTCCTATGTTGTGAAAGCAAAGGAACAAAGAAAAGAAAAGAAAAGAAAAAAATGAAAATCACACAAAAAATGAAAAAAGAGAATTTCTGTGAAGAAAACCTTATTGTATTTAGTAGTGTATCTCCCAAAGTCCAAAGAATTGAAGGAGGAAAGGCTACTTATAGGAAAATGAACTTAAATGTAGAGTTTATCTGTTGAGCTTTATTTTATTATTATTATTATTAATTATTATTATTTTCATCTTCTTTCTTTCA

Coding sequence (CDS)

ATGGAGCTTCTATCGGCGGCTCTGTTTGGCCTTCTCTTGGCTTCCATCCTTCCTCTCATTTTCTTCTTCGCTTTCAAAGATGGAATCAACAGCTCCTCCCCTCCCAATCCCATCCATTCTAAATCTATCAATTCCCCCTCCGCCGCCGCCCTACAGATTCCCCCCCACTCCGATGACGACCACCCTGACCTCATCGTCGTCGGCGCTGGTGTCGCCGGCGCCGCTCTTGCTTTTACTCTCGCCAAGGATGGAAGAAAAGTGCACGTAATCGAGAGGGACTTGACGGAGCCAGATCGAATCGTCGGAGAATTGCTTCAACCCGGCGGCTATTTGAAGCTCATCGAGTTGGGGCTTGAAGACAGTCTGAACGGAATCGATGCTCAGCAGGTGTTTGGTTATGCCCTCTACAAGAATGGCAGTCACACCAGATTGGCTTATCCTTTGGAGAAATTTGATTCCAATGTTTCTGGAAGAAGCTTTCACAATGGCCGTTTCATTCAGAGATTGAGGGAAAAGGCTGCTGCTCTTTCAAATGTGAGAATGGAACAAGGGACTGTAACGTCCATAGTTGAAGAAGATGGGATTGTGAAAGGTGTGCAATATAAAACCAAGAACGGCAAAGAGTTGACAGCTTATGCTCCCCTCACAATTGTTTGTGATGGTGGCTTTTCGAATTTGCGTCGCAAGCTCTGCAAACCTCAGATTGACATTCCCTCATGCTTTGTTGGTTTGGTCCTGGAGAACTGCAAGCTTCCCCTTGAAAATCATGGACATGTTGTGCTAGCAGATCCATCACCTATCTTGTTATACCCGATTAGCAGCACAGAGGTACGCTGCTTAGTCGATATCCCCGGCCAAAAAGTCCCTTCTGTTGCTAATGGTGAAATGGCACACTACTTGAAAACTGCTGTAGCTCCTCAGCTACCGCCTGAGGTGTATCCTGCCTTCATTGCTGCAATTGATAAAGGAAACATAAAAAGCACAACAAATAGAAGTATGCCAGCAGCACCACAATCCACGCCAGGAGCACTTCTCTTGGGTGATGCATTCAACATGCGACATCCTTTAACAGGTGGAGGAATGACGGTTGCTCTCTCTGATATCGTTGTGCTACGAGACCTTCTTCGACCCCTTTCTAATTTAAACGATGCTGATGTCTTGTGCAACTATCTCGAATCCTTCTACACTTTGCGCAAGCCAGTGGCATCTACAATAAACACATTAGCAGGTGCCTTATACAAGGTGTTTTGTGCATCACCAGATCCAGCCAGGAAGGAAATGCGCGAAGCTTGTTTCGATTATTTGAGTTTAGGAGGCGTGTTTTCATCAGGACCAGTGGCCCTTCTCTCTGGTCTTAACCCACGCCCATTAAGCTTGTTCTGTCACTTCTTTGCTGTTGCTATATACGGTGTTGGTCGGTTGCTAATCCCATTCCCCTCACCTAAGCGTATATGGATTGGAGTTCGGTTGATTACGGGAGCAGCGGGCATAATTTTTCCCATAATAAAGGATGAAGGAGTGAGACAAATGTTCTTCCCCGCAACAGTGCCTGCATATTACAGAACACCTCCTATGTTGTGA

Protein sequence

MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDDHPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRMEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSCFVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYLKTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGGMTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSPKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPML
Homology
BLAST of Cmc01g0004571 vs. NCBI nr
Match: KAA0031396.1 (squalene monooxygenase-like [Cucumis melo var. makuwa] >TYK06847.1 squalene monooxygenase-like [Cucumis melo var. makuwa])

HSP 1 Score: 1048.9 bits (2711), Expect = 1.5e-302
Identity = 522/526 (99.24%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           MELLSAALFGLLLASILPLIFFFAFKDGIN+SSPPNPIHSKSINSPSAAALQIPPHSDDD
Sbjct: 1   MELLSAALFGLLLASILPLIFFFAFKDGINNSSPPNPIHSKSINSPSAAALQIPPHSDDD 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
           HPD+IVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED
Sbjct: 61  HPDVIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR
Sbjct: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC
Sbjct: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPML 527
           PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPP++
Sbjct: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPIV 526

BLAST of Cmc01g0004571 vs. NCBI nr
Match: XP_004136919.1 (squalene monooxygenase SE2 [Cucumis sativus] >KGN43821.1 hypothetical protein Csa_017228 [Cucumis sativus])

HSP 1 Score: 1002.7 bits (2591), Expect = 1.2e-288
Identity = 502/526 (95.44%), Postives = 515/526 (97.91%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           MELLSAALFGLLLASILPLIFFFAF+DGIN+SS PNPIH KSINSP  A LQIPPHS+DD
Sbjct: 1   MELLSAALFGLLLASILPLIFFFAFRDGINNSSAPNPIHPKSINSP--APLQIPPHSNDD 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
           +PD+IVVGAGVAGAALAFTLAKDGR+VHVIERDLTEPDRIVGELLQPGGYLKLIELGLED
Sbjct: 61  NPDVIVVGAGVAGAALAFTLAKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SLNGI+AQQVFGYALYKNG HTRLAYPLEK+DS+VSGRSFHNGRFIQRLREKAAA SNVR
Sbjct: 121 SLNGIEAQQVFGYALYKNGRHTRLAYPLEKYDSSVSGRSFHNGRFIQRLREKAAAFSNVR 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC
Sbjct: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLYPIS+TEVRCLVDIPGQKVPSVANGEMAHYL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISNTEVRCLVDIPGQKVPSVANGEMAHYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KTAVAPQLPPEVYP+FIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTAVAPQLPPEVYPSFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVALSDIV+LRDLLRPLSNLNDADVL NYLESFYTLRKPVASTINTLAGALY+VF ASP
Sbjct: 361 MTVALSDIVLLRDLLRPLSNLNDADVLSNYLESFYTLRKPVASTINTLAGALYRVFSASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPML 527
           PKRIWIGVRLIT AAGIIFPIIK EGVRQMFFPATVPAYYRTPPML
Sbjct: 481 PKRIWIGVRLITVAAGIIFPIIKAEGVRQMFFPATVPAYYRTPPML 524

BLAST of Cmc01g0004571 vs. NCBI nr
Match: XP_038887697.1 (squalene monooxygenase SE2-like [Benincasa hispida])

HSP 1 Score: 957.6 bits (2474), Expect = 4.5e-275
Identity = 481/526 (91.44%), Postives = 500/526 (95.06%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSD-D 60
           ME LSAALFGLLLASILPL+FFFAF+DGIN+++    + SKS NS    A+QIP  S+  
Sbjct: 1   MEFLSAALFGLLLASILPLLFFFAFRDGINNAAAAPAVDSKSTNS----AVQIPLCSETS 60

Query: 61  DHPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLE 120
           D PD+IVVGAGVAGAALAFTLAKDGR+VHVIERDLTEPDRIVGELLQPGGYLKLIELGLE
Sbjct: 61  DDPDVIVVGAGVAGAALAFTLAKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLE 120

Query: 121 DSLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNV 180
           DSL GIDAQQVFGYALYK+G+HTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNV
Sbjct: 121 DSLKGIDAQQVFGYALYKDGNHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNV 180

Query: 181 RMEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPS 240
           R+EQGTVTS+VEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPS
Sbjct: 181 RLEQGTVTSVVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPS 240

Query: 241 CFVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHY 300
           CFVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMA Y
Sbjct: 241 CFVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAQY 300

Query: 301 LKTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGG 360
           LKT VAPQLPPE+Y AFIAAI+KGNIKSTTNRSMPA P  TPGALLLGDAFNMRHPLTGG
Sbjct: 301 LKTVVAPQLPPELYAAFIAAIEKGNIKSTTNRSMPAIPHPTPGALLLGDAFNMRHPLTGG 360

Query: 361 GMTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCAS 420
           GMTVAL+DIV+LRDLLRPLSNL DAD LCNYLESFYTLRKPVASTINTLAGALY+VFCAS
Sbjct: 361 GMTVALADIVLLRDLLRPLSNLKDADALCNYLESFYTLRKPVASTINTLAGALYRVFCAS 420

Query: 421 PDPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFP 480
           PDPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFP
Sbjct: 421 PDPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFP 480

Query: 481 SPKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPM 526
           SPKRIWIGVRLITGAAGIIFPIIK EGVRQMFFPATVPAYYRTPPM
Sbjct: 481 SPKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATVPAYYRTPPM 522

BLAST of Cmc01g0004571 vs. NCBI nr
Match: XP_023553953.1 (squalene epoxidase 1-like isoform X1 [Cucurbita pepo subsp. pepo] >AXK50228.1 squalene epoxidase 2 [Cucurbita pepo])

HSP 1 Score: 916.0 bits (2366), Expect = 1.5e-262
Identity = 457/525 (87.05%), Postives = 485/525 (92.38%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           ME LSAAL GLLLASILPL FFF+F+DGI      N         PS   ++    SDD 
Sbjct: 1   MEFLSAALLGLLLASILPLFFFFSFRDGIKHDGDNNTTAVIDSKIPSGNDVRRAESSDDF 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
             D+I+VGAGVAGAALA+TLAKDGRKVHVIERDL+EP+RIVGELLQPGGYLKLIELGLED
Sbjct: 61  --DVIIVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SL GIDAQQVFGYAL+K+G+HTRLAYPL+KFDSN+SGRSFHNGRFIQRLR+KAAALSNV+
Sbjct: 121 SLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSNVK 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           +EQGTVTSIVEEDG VKGVQYKTKNG+ELT+YAPLTIVCDGGFSNLRR LCKPQIDIPSC
Sbjct: 181 LEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLY ISSTE+RCLVDIPGQKVPSVANG+MAHYL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAHYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KTAVAPQLPPE+  AFIA++DKGNIKSTTNRSMPAAP  TPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTAVAPQLPPELQTAFIASVDKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVAL+DIVVLRDLLRPLSNLNDAD LCNYLESFYTLRKPVASTINTLAGALY+VFCASP
Sbjct: 361 MTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV RLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPM 526
           PKRIWIGVRLITGAAGIIFPIIK EGVRQMFFPAT+PAYYR PPM
Sbjct: 481 PKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 523

BLAST of Cmc01g0004571 vs. NCBI nr
Match: XP_022952179.1 (squalene epoxidase 1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 913.3 bits (2359), Expect = 9.6e-262
Identity = 455/525 (86.67%), Postives = 484/525 (92.19%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           ME LSAAL GLLLASILPL FFF+F+DGI      N   +  I+S   +   +      D
Sbjct: 1   MEFLSAALLGLLLASILPLFFFFSFRDGIKHDGDNNTT-TAVIDSKIPSGNDVRRADSSD 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
             D+IVVGAGVAGAALA+TLAKDGRKVHVIERDL+EP+RIVGELLQPGGYLKLIELGLED
Sbjct: 61  DLDVIVVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SL GIDAQQVFGYAL+K+G+HTRLAYPL+KFDSN+SGRSFHNGRFIQRLR+KAAALSNV+
Sbjct: 121 SLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSNVK 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           +EQGTVTSIVEEDG VKGVQYKTKNG+ELT+YAPLTIVCDGGFSNLRR LCKPQIDIPSC
Sbjct: 181 LEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLY ISSTE+RCLVDIPGQKVPSVANG+MAHYL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAHYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KT VAPQLPPE+  AFIA++DKGNIKSTTNRSMPAAP  TPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQLPPELQTAFIASVDKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVAL+DIVVLRDLLRPLSNLNDAD LCNYLESFYTLRKPVASTINTLAGALY+VFCASP
Sbjct: 361 MTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV RLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPM 526
           PKRIWIGVRLITGAAGIIFPIIK EGVRQMFFPAT+PAYYR PPM
Sbjct: 481 PKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 524

BLAST of Cmc01g0004571 vs. ExPASy Swiss-Prot
Match: B7TWW5 (Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2)

HSP 1 Score: 786.9 bits (2031), Expect = 1.4e-226
Identity = 374/464 (80.60%), Postives = 427/464 (92.03%), Query Frame = 0

Query: 63  DLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSL 122
           D+I+VGAGVAG+ALA+TLAKDGR+VHVIERDLTE DRIVGELLQPGGYLKL+ELGLED +
Sbjct: 78  DVIIVGAGVAGSALAYTLAKDGRRVHVIERDLTEQDRIVGELLQPGGYLKLVELGLEDCV 137

Query: 123 NGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRME 182
           N IDAQ+VFGYALY +G +TRL+YPLEKF ++V+GRSFHNGRFIQR+REKAA+L NVRME
Sbjct: 138 NEIDAQRVFGYALYMDGKNTRLSYPLEKFHADVAGRSFHNGRFIQRMREKAASLPNVRME 197

Query: 183 QGTVTSIVEEDGIVKGVQYKTKNGKELT-AYAPLTIVCDGGFSNLRRKLCKPQIDIPSCF 242
           QGTVTS+VE+ G VKGV+YKTKNG+E++ AYAPLTIVCDG FSNLR  LC P++D+PSCF
Sbjct: 198 QGTVTSLVEQKGTVKGVRYKTKNGQEMSAAYAPLTIVCDGCFSNLRHSLCNPKVDVPSCF 257

Query: 243 VGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYLK 302
           VGL+LEN  LP  NHGHV+LADPSPIL Y ISSTE+RCLVD+PGQKVPS+ANGE+AHYLK
Sbjct: 258 VGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPSIANGELAHYLK 317

Query: 303 TAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGGM 362
           T+VAPQ+PPE+Y +FIAAIDKG IK+  NRSMPA P STPGALLLGDAFNMRHPLTGGGM
Sbjct: 318 TSVAPQIPPELYKSFIAAIDKGKIKTMPNRSMPADPHSTPGALLLGDAFNMRHPLTGGGM 377

Query: 363 TVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASPD 422
           TVALSDIV++RDLLRPL +L+D+  LC YLESFYTLRKPVASTINTLAGALYKVFCASPD
Sbjct: 378 TVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPD 437

Query: 423 PARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSP 482
            AR+EMR+ACFDYLSLGG+ S GP+ALLSGLNPRP+SLF HFFAVAIYGVGRLLIPFPSP
Sbjct: 438 KARQEMRDACFDYLSLGGICSEGPIALLSGLNPRPMSLFFHFFAVAIYGVGRLLIPFPSP 497

Query: 483 KRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPM 526
           +++W+G RLI+GA+GIIFPIIK EGVRQMFFPATVPAYYR PP+
Sbjct: 498 RKMWLGARLISGASGIIFPIIKSEGVRQMFFPATVPAYYRAPPI 541

BLAST of Cmc01g0004571 vs. ExPASy Swiss-Prot
Match: O48651 (Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1)

HSP 1 Score: 774.6 bits (1999), Expect = 7.0e-223
Identity = 367/463 (79.27%), Postives = 421/463 (90.93%), Query Frame = 0

Query: 63  DLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSL 122
           D+I+VGAGVAG+ALA+TLA DGR+VHVIERDLTE DRIVGELLQPGGYLKLIELGLED +
Sbjct: 76  DVIIVGAGVAGSALAYTLANDGRRVHVIERDLTEQDRIVGELLQPGGYLKLIELGLEDCV 135

Query: 123 NGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRME 182
           N IDAQ+VFGYALY +G +TRL+YPLEKF S+V+GRSFHNGRF+QR+REKAA+L NVRME
Sbjct: 136 NEIDAQRVFGYALYMDGKNTRLSYPLEKFHSDVAGRSFHNGRFVQRMREKAASLPNVRME 195

Query: 183 QGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSCFV 242
           QGTVTS+VE+ G VKGVQYKTK+G+EL+A+APLTIVCDG FSNLRR LC P++++PSCFV
Sbjct: 196 QGTVTSLVEKKGSVKGVQYKTKDGQELSAFAPLTIVCDGCFSNLRRSLCNPKVEVPSCFV 255

Query: 243 GLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYLKT 302
           GL+LEN  LP  NHGHV+LADPSPIL Y ISSTE+RCLVD+PGQKVP ++NGE+A+YLKT
Sbjct: 256 GLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPCISNGELANYLKT 315

Query: 303 AVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGGMT 362
            VAPQ+P ++Y +FIAA+DKGNI++  NRSMPA P  TPGALLLGDAFNMRHPLTGGGMT
Sbjct: 316 VVAPQVPKQLYNSFIAAVDKGNIRTMPNRSMPADPHPTPGALLLGDAFNMRHPLTGGGMT 375

Query: 363 VALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASPDP 422
           VALSDIV++RDLLRPL +L+D+  LC YLESFYTLRKPVASTINTLAGALYKVFCASPD 
Sbjct: 376 VALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFCASPDK 435

Query: 423 ARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSPK 482
           AR+EMR ACFDYLSLGG+ S GP+ALLSGLNPRP+SLF HFFAVAIYGVGRLLIPFPSPK
Sbjct: 436 ARQEMRNACFDYLSLGGICSQGPIALLSGLNPRPISLFLHFFAVAIYGVGRLLIPFPSPK 495

Query: 483 RIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPM 526
           R+W+G RLI GA+GIIFPIIK EG+RQMFFPA VPAYYR PP+
Sbjct: 496 RMWLGARLILGASGIIFPIIKSEGLRQMFFPAIVPAYYRAPPI 538

BLAST of Cmc01g0004571 vs. ExPASy Swiss-Prot
Match: Q8VYH2 (Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.7e-216
Identity = 359/462 (77.71%), Postives = 414/462 (89.61%), Query Frame = 0

Query: 63  DLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSL 122
           D+I+VGAGVAGAALA TL K+GR+VHVIERDLTEPDRIVGELLQPGGYLKLIELGLED +
Sbjct: 56  DIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDCV 115

Query: 123 NGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRME 182
             IDAQ+V GYAL+K+G HT+L+YPL++FDS+V+GRSFHNGRF+QR+REKA+ L NVRME
Sbjct: 116 KDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNVRME 175

Query: 183 QGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSCFV 242
           QGTVTS+VEE+GI+KGVQYKTK+G+EL ++APLTIVCDG FSNLRR LCKP++++PS FV
Sbjct: 176 QGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPSNFV 235

Query: 243 GLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYLKT 302
           GLVLENC+LP  NHGHVVL DPSPIL YPISS+EVRCLVD+PG K+PSVA+GEMAH+LKT
Sbjct: 236 GLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHHLKT 295

Query: 303 AVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGGMT 362
            VAPQ+PP++  AFI+A++KGNI++  NRSMPA P  TPGALLLGDAFNMRHPLTGGGMT
Sbjct: 296 MVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGGGMT 355

Query: 363 VALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASPDP 422
           VALSDIV+LRDLL PL +L + + L  Y+ESFYTLRKPVASTINTLAGALYKVF ASPD 
Sbjct: 356 VALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLASPDD 415

Query: 423 ARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSPK 482
           AR EMR ACFDYLSLGGV SSGPVALLSGLNPRP+SL  HFFAVAI+GVGRLL+P PS K
Sbjct: 416 ARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPSVK 475

Query: 483 RIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPP 525
           R+W+G RLI+ A+GIIFPIIK EGVRQMFFP T+PA YR PP
Sbjct: 476 RLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of Cmc01g0004571 vs. ExPASy Swiss-Prot
Match: Q9SM02 (Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1)

HSP 1 Score: 751.9 bits (1940), Expect = 4.9e-216
Identity = 372/514 (72.37%), Postives = 431/514 (83.85%), Query Frame = 0

Query: 11  LLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDDHPDLIVVGAG 70
           LL++S+  LI F AF             H +   S   + +     + D   D+IVVGAG
Sbjct: 12  LLISSL--LISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDVGSVNITGDTVADVIVVGAG 71

Query: 71  VAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSLNGIDAQQV 130
           VAG+ALA+TL KD R+VHVIERDL+EPDRIVGELLQPGGYLKL+ELG+ED +  IDAQ+V
Sbjct: 72  VAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIEDCVEEIDAQRV 131

Query: 131 FGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRMEQGTVTSIV 190
           +GYAL+KNG   RLAYPLEKF  +VSGRSFHNGRFIQR+REKAA+L NV++EQGTV S++
Sbjct: 132 YGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQLEQGTVLSLL 191

Query: 191 EEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSCFVGLVLENCK 250
           EE+G +KGV+YK K G+E TA+A LTIVCDG FSNLRR LC PQ+++PSCFVGLVLENC 
Sbjct: 192 EENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSCFVGLVLENCN 251

Query: 251 LPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYLKTAVAPQLPP 310
           LP  NHGHVVLADPSPIL+YPISSTEVRCLVD+PGQKVPS+ANGEM +YLKT VAPQ+P 
Sbjct: 252 LPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYLKTVVAPQMPH 311

Query: 311 EVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGGMTVALSDIVV 370
           EVY +FIAA+DKGNIKS  NRSMPA+P  TPGALL+GDAFNMRHPLTGGGMTVAL+DIVV
Sbjct: 312 EVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALADIVV 371

Query: 371 LRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREA 430
           LR+LLRPL +L+D   LC YLESFYTLRKPVA+TINTLA ALY+VFC+S + AR EMREA
Sbjct: 372 LRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSENEARNEMREA 431

Query: 431 CFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSPKRIWIGVRL 490
           CFDYL LGG+ +SGPV+LLSGLNPRPL+L CHFFAVA+YGV RLLIPFPSPKRIW+G +L
Sbjct: 432 CFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPSPKRIWLGAKL 491

Query: 491 ITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPP 525
           I+GA+GIIFPIIK EGVRQMFFPATVPAYY   P
Sbjct: 492 ISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of Cmc01g0004571 vs. ExPASy Swiss-Prot
Match: O81000 (Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 1.8e-210
Identity = 352/466 (75.54%), Postives = 402/466 (86.27%), Query Frame = 0

Query: 59  DDHPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 118
           D   D+I+VGAGVAG+ALA TL K+GR+VHVIERD +E DRIVGELLQPGGYLKLIELGL
Sbjct: 120 DSGTDVIIVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELGL 179

Query: 119 EDSLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSN 178
           ED +  IDAQ+V GY L+K+G HT+LAYPLE FDS+V+GRSFHNGRF+QR+REKA  LSN
Sbjct: 180 EDCVKKIDAQRVLGYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLSN 239

Query: 179 VRMEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIP 238
           VR+EQGTVTS++EE G +KGV+Y+TK G E  ++APLTIVCDG FSNLRR LCKP++D+P
Sbjct: 240 VRLEQGTVTSLLEEHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDVP 299

Query: 239 SCFVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAH 298
           S FVGLVLENC+LP  NHGHVVL DPSPIL+YPISS+EVRCLVD+PGQK+P +ANGEMA 
Sbjct: 300 STFVGLVLENCELPFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMAK 359

Query: 299 YLKTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTG 358
           YLKT VAPQ+P +V  AFI A++KGNI++  NRSMPA P  TPGALLLGDAFNMRHPLTG
Sbjct: 360 YLKTRVAPQVPTKVREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLTG 419

Query: 359 GGMTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCA 418
           GGMTVAL+DIVVLRDLLRP+ NLND + L  Y+ESFYTLRKPVASTINTLA ALYKVF A
Sbjct: 420 GGMTVALADIVVLRDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFLA 479

Query: 419 SPDPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPF 478
           S D AR EMREACFDYLSLGGVFSSGPVALLSGLNPRPLSL  HFFAVAIY V RL++PF
Sbjct: 480 SSDEARTEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPF 539

Query: 479 PSPKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPP 525
           PS +  W+G R+I+ A+ IIFPIIK EGVRQMFFP T+PA YR PP
Sbjct: 540 PSIESFWLGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of Cmc01g0004571 vs. ExPASy TrEMBL
Match: A0A5A7SMZ2 (Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold13G001510 PE=3 SV=1)

HSP 1 Score: 1048.9 bits (2711), Expect = 7.1e-303
Identity = 522/526 (99.24%), Postives = 526/526 (100.00%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           MELLSAALFGLLLASILPLIFFFAFKDGIN+SSPPNPIHSKSINSPSAAALQIPPHSDDD
Sbjct: 1   MELLSAALFGLLLASILPLIFFFAFKDGINNSSPPNPIHSKSINSPSAAALQIPPHSDDD 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
           HPD+IVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED
Sbjct: 61  HPDVIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR
Sbjct: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC
Sbjct: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP
Sbjct: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPML 527
           PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPP++
Sbjct: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPIV 526

BLAST of Cmc01g0004571 vs. ExPASy TrEMBL
Match: A0A0A0K7N0 (Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_7G069710 PE=3 SV=1)

HSP 1 Score: 1002.7 bits (2591), Expect = 5.8e-289
Identity = 502/526 (95.44%), Postives = 515/526 (97.91%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           MELLSAALFGLLLASILPLIFFFAF+DGIN+SS PNPIH KSINSP  A LQIPPHS+DD
Sbjct: 1   MELLSAALFGLLLASILPLIFFFAFRDGINNSSAPNPIHPKSINSP--APLQIPPHSNDD 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
           +PD+IVVGAGVAGAALAFTLAKDGR+VHVIERDLTEPDRIVGELLQPGGYLKLIELGLED
Sbjct: 61  NPDVIVVGAGVAGAALAFTLAKDGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SLNGI+AQQVFGYALYKNG HTRLAYPLEK+DS+VSGRSFHNGRFIQRLREKAAA SNVR
Sbjct: 121 SLNGIEAQQVFGYALYKNGRHTRLAYPLEKYDSSVSGRSFHNGRFIQRLREKAAAFSNVR 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC
Sbjct: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLYPIS+TEVRCLVDIPGQKVPSVANGEMAHYL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISNTEVRCLVDIPGQKVPSVANGEMAHYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KTAVAPQLPPEVYP+FIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTAVAPQLPPEVYPSFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVALSDIV+LRDLLRPLSNLNDADVL NYLESFYTLRKPVASTINTLAGALY+VF ASP
Sbjct: 361 MTVALSDIVLLRDLLRPLSNLNDADVLSNYLESFYTLRKPVASTINTLAGALYRVFSASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPML 527
           PKRIWIGVRLIT AAGIIFPIIK EGVRQMFFPATVPAYYRTPPML
Sbjct: 481 PKRIWIGVRLITVAAGIIFPIIKAEGVRQMFFPATVPAYYRTPPML 524

BLAST of Cmc01g0004571 vs. ExPASy TrEMBL
Match: A0A345Z0U9 (Squalene monooxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 916.0 bits (2366), Expect = 7.2e-263
Identity = 457/525 (87.05%), Postives = 485/525 (92.38%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           ME LSAAL GLLLASILPL FFF+F+DGI      N         PS   ++    SDD 
Sbjct: 1   MEFLSAALLGLLLASILPLFFFFSFRDGIKHDGDNNTTAVIDSKIPSGNDVRRAESSDDF 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
             D+I+VGAGVAGAALA+TLAKDGRKVHVIERDL+EP+RIVGELLQPGGYLKLIELGLED
Sbjct: 61  --DVIIVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SL GIDAQQVFGYAL+K+G+HTRLAYPL+KFDSN+SGRSFHNGRFIQRLR+KAAALSNV+
Sbjct: 121 SLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSNVK 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           +EQGTVTSIVEEDG VKGVQYKTKNG+ELT+YAPLTIVCDGGFSNLRR LCKPQIDIPSC
Sbjct: 181 LEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLY ISSTE+RCLVDIPGQKVPSVANG+MAHYL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAHYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KTAVAPQLPPE+  AFIA++DKGNIKSTTNRSMPAAP  TPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTAVAPQLPPELQTAFIASVDKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVAL+DIVVLRDLLRPLSNLNDAD LCNYLESFYTLRKPVASTINTLAGALY+VFCASP
Sbjct: 361 MTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV RLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPM 526
           PKRIWIGVRLITGAAGIIFPIIK EGVRQMFFPAT+PAYYR PPM
Sbjct: 481 PKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 523

BLAST of Cmc01g0004571 vs. ExPASy TrEMBL
Match: A0A6J1GKV9 (Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111454931 PE=3 SV=1)

HSP 1 Score: 913.3 bits (2359), Expect = 4.7e-262
Identity = 455/525 (86.67%), Postives = 484/525 (92.19%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           ME LSAAL GLLLASILPL FFF+F+DGI      N   +  I+S   +   +      D
Sbjct: 1   MEFLSAALLGLLLASILPLFFFFSFRDGIKHDGDNNTT-TAVIDSKIPSGNDVRRADSSD 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
             D+IVVGAGVAGAALA+TLAKDGRKVHVIERDL+EP+RIVGELLQPGGYLKLIELGLED
Sbjct: 61  DLDVIVVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SL GIDAQQVFGYAL+K+G+HTRLAYPL+KFDSN+SGRSFHNGRFIQRLR+KAAALSNV+
Sbjct: 121 SLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSNVK 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           +EQGTVTSIVEEDG VKGVQYKTKNG+ELT+YAPLTIVCDGGFSNLRR LCKPQIDIPSC
Sbjct: 181 LEQGTVTSIVEEDGTVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLY ISSTE+RCLVDIPGQKVPSVANG+MAHYL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMAHYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KT VAPQLPPE+  AFIA++DKGNIKSTTNRSMPAAP  TPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQLPPELQTAFIASVDKGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVAL+DIVVLRDLLRPLSNLNDAD LCNYLESFYTLRKPVASTINTLAGALY+VFCASP
Sbjct: 361 MTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV RLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPM 526
           PKRIWIGVRLITGAAGIIFPIIK EGVRQMFFPAT+PAYYR PPM
Sbjct: 481 PKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 524

BLAST of Cmc01g0004571 vs. ExPASy TrEMBL
Match: A0A6J1I4X3 (Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111471032 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.6e-260
Identity = 454/525 (86.48%), Postives = 482/525 (91.81%), Query Frame = 0

Query: 1   MELLSAALFGLLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDD 60
           ME LSAAL GLLLASILPL FFF+F+DGI      N         PS   ++    SDD 
Sbjct: 1   MEFLSAALLGLLLASILPLFFFFSFRDGIKHDGDNNTTTVIDSKIPSGNDVRHAESSDDF 60

Query: 61  HPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 120
             D+IVVGAGVAGAALA+TLAKDGRKVHVIERDL+EP+RIVGELLQPGGYLKLIELGLED
Sbjct: 61  --DVIVVGAGVAGAALAYTLAKDGRKVHVIERDLSEPERIVGELLQPGGYLKLIELGLED 120

Query: 121 SLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVR 180
           SL GIDAQQVFGYAL+K+G+HTRLAYPL+KFDSN+SGRSFHNGRFIQRLR+KAAALSNV+
Sbjct: 121 SLKGIDAQQVFGYALFKDGNHTRLAYPLDKFDSNISGRSFHNGRFIQRLRQKAAALSNVK 180

Query: 181 MEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSC 240
           +EQGTVTSIVEEDG VKGVQYKTKNG+ELT+YAPLTIVCDGGFSNLRR LCKPQIDIPSC
Sbjct: 181 LEQGTVTSIVEEDGNVKGVQYKTKNGQELTSYAPLTIVCDGGFSNLRRNLCKPQIDIPSC 240

Query: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYL 300
           FVGLVLENCKLPLENHGHVVLADPSPILLY ISSTE+RCLVDIPGQKVPSVANG+MA YL
Sbjct: 241 FVGLVLENCKLPLENHGHVVLADPSPILLYRISSTEIRCLVDIPGQKVPSVANGDMARYL 300

Query: 301 KTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGG 360
           KT VAPQLPPE+  AFIA+++ GNIKSTTNRSMPAAP  TPGALLLGDAFNMRHPLTGGG
Sbjct: 301 KTVVAPQLPPELQTAFIASVENGNIKSTTNRSMPAAPHPTPGALLLGDAFNMRHPLTGGG 360

Query: 361 MTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASP 420
           MTVAL+DIVVLRDLLRPLSNLNDAD LCNYLESFYTLRKPVASTINTLAGALY+VFCASP
Sbjct: 361 MTVALADIVVLRDLLRPLSNLNDADALCNYLESFYTLRKPVASTINTLAGALYQVFCASP 420

Query: 421 DPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPS 480
           DPARKEMREACFDYLSLGGVFSSGPV+LLSGLNPRPLSLFCHFFAVAIYGV RLLIPFPS
Sbjct: 421 DPARKEMREACFDYLSLGGVFSSGPVSLLSGLNPRPLSLFCHFFAVAIYGVTRLLIPFPS 480

Query: 481 PKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPPM 526
           PKRIWIGVRLITGAAGIIFPIIK EGVRQMFFPAT+PAYYR PPM
Sbjct: 481 PKRIWIGVRLITGAAGIIFPIIKAEGVRQMFFPATMPAYYRAPPM 523

BLAST of Cmc01g0004571 vs. TAIR 10
Match: AT4G37760.1 (squalene epoxidase 3 )

HSP 1 Score: 753.4 bits (1944), Expect = 1.2e-217
Identity = 359/462 (77.71%), Postives = 414/462 (89.61%), Query Frame = 0

Query: 63  DLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSL 122
           D+I+VGAGVAGAALA TL K+GR+VHVIERDLTEPDRIVGELLQPGGYLKLIELGLED +
Sbjct: 56  DIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDCV 115

Query: 123 NGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRME 182
             IDAQ+V GYAL+K+G HT+L+YPL++FDS+V+GRSFHNGRF+QR+REKA+ L NVRME
Sbjct: 116 KDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNVRME 175

Query: 183 QGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSCFV 242
           QGTVTS+VEE+GI+KGVQYKTK+G+EL ++APLTIVCDG FSNLRR LCKP++++PS FV
Sbjct: 176 QGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPSNFV 235

Query: 243 GLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYLKT 302
           GLVLENC+LP  NHGHVVL DPSPIL YPISS+EVRCLVD+PG K+PSVA+GEMAH+LKT
Sbjct: 236 GLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHHLKT 295

Query: 303 AVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGGMT 362
            VAPQ+PP++  AFI+A++KGNI++  NRSMPA P  TPGALLLGDAFNMRHPLTGGGMT
Sbjct: 296 MVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGGGMT 355

Query: 363 VALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASPDP 422
           VALSDIV+LRDLL PL +L + + L  Y+ESFYTLRKPVASTINTLAGALYKVF ASPD 
Sbjct: 356 VALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLASPDD 415

Query: 423 ARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSPK 482
           AR EMR ACFDYLSLGGV SSGPVALLSGLNPRP+SL  HFFAVAI+GVGRLL+P PS K
Sbjct: 416 ARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPSVK 475

Query: 483 RIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPP 525
           R+W+G RLI+ A+GIIFPIIK EGVRQMFFP T+PA YR PP
Sbjct: 476 RLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of Cmc01g0004571 vs. TAIR 10
Match: AT1G58440.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 751.9 bits (1940), Expect = 3.5e-217
Identity = 372/514 (72.37%), Postives = 431/514 (83.85%), Query Frame = 0

Query: 11  LLLASILPLIFFFAFKDGINSSSPPNPIHSKSINSPSAAALQIPPHSDDDHPDLIVVGAG 70
           LL++S+  LI F AF             H +   S   + +     + D   D+IVVGAG
Sbjct: 12  LLISSL--LISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDVGSVNITGDTVADVIVVGAG 71

Query: 71  VAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGLEDSLNGIDAQQV 130
           VAG+ALA+TL KD R+VHVIERDL+EPDRIVGELLQPGGYLKL+ELG+ED +  IDAQ+V
Sbjct: 72  VAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLLELGIEDCVEEIDAQRV 131

Query: 131 FGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSNVRMEQGTVTSIV 190
           +GYAL+KNG   RLAYPLEKF  +VSGRSFHNGRFIQR+REKAA+L NV++EQGTV S++
Sbjct: 132 YGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAASLPNVQLEQGTVLSLL 191

Query: 191 EEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIPSCFVGLVLENCK 250
           EE+G +KGV+YK K G+E TA+A LTIVCDG FSNLRR LC PQ+++PSCFVGLVLENC 
Sbjct: 192 EENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQVEVPSCFVGLVLENCN 251

Query: 251 LPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAHYLKTAVAPQLPP 310
           LP  NHGHVVLADPSPIL+YPISSTEVRCLVD+PGQKVPS+ANGEM +YLKT VAPQ+P 
Sbjct: 252 LPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANGEMKNYLKTVVAPQMPH 311

Query: 311 EVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTGGGMTVALSDIVV 370
           EVY +FIAA+DKGNIKS  NRSMPA+P  TPGALL+GDAFNMRHPLTGGGMTVAL+DIVV
Sbjct: 312 EVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRHPLTGGGMTVALADIVV 371

Query: 371 LRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCASPDPARKEMREA 430
           LR+LLRPL +L+D   LC YLESFYTLRKPVA+TINTLA ALY+VFC+S + AR EMREA
Sbjct: 372 LRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQVFCSSENEARNEMREA 431

Query: 431 CFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPFPSPKRIWIGVRL 490
           CFDYL LGG+ +SGPV+LLSGLNPRPL+L CHFFAVA+YGV RLLIPFPSPKRIW+G +L
Sbjct: 432 CFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRLLIPFPSPKRIWLGAKL 491

Query: 491 ITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPP 525
           I+GA+GIIFPIIK EGVRQMFFPATVPAYY   P
Sbjct: 492 ISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of Cmc01g0004571 vs. TAIR 10
Match: AT2G22830.1 (squalene epoxidase 2 )

HSP 1 Score: 733.4 bits (1892), Expect = 1.3e-211
Identity = 352/466 (75.54%), Postives = 402/466 (86.27%), Query Frame = 0

Query: 59  DDHPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 118
           D   D+I+VGAGVAG+ALA TL K+GR+VHVIERD +E DRIVGELLQPGGYLKLIELGL
Sbjct: 120 DSGTDVIIVGAGVAGSALAHTLGKEGRRVHVIERDFSEQDRIVGELLQPGGYLKLIELGL 179

Query: 119 EDSLNGIDAQQVFGYALYKNGSHTRLAYPLEKFDSNVSGRSFHNGRFIQRLREKAAALSN 178
           ED +  IDAQ+V GY L+K+G HT+LAYPLE FDS+V+GRSFHNGRF+QR+REKA  LSN
Sbjct: 180 EDCVKKIDAQRVLGYVLFKDGKHTKLAYPLETFDSDVAGRSFHNGRFVQRMREKALTLSN 239

Query: 179 VRMEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQIDIP 238
           VR+EQGTVTS++EE G +KGV+Y+TK G E  ++APLTIVCDG FSNLRR LCKP++D+P
Sbjct: 240 VRLEQGTVTSLLEEHGTIKGVRYRTKEGNEFRSFAPLTIVCDGCFSNLRRSLCKPKVDVP 299

Query: 239 SCFVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGEMAH 298
           S FVGLVLENC+LP  NHGHVVL DPSPIL+YPISS+EVRCLVD+PGQK+P +ANGEMA 
Sbjct: 300 STFVGLVLENCELPFANHGHVVLGDPSPILMYPISSSEVRCLVDVPGQKLPPIANGEMAK 359

Query: 299 YLKTAVAPQLPPEVYPAFIAAIDKGNIKSTTNRSMPAAPQSTPGALLLGDAFNMRHPLTG 358
           YLKT VAPQ+P +V  AFI A++KGNI++  NRSMPA P  TPGALLLGDAFNMRHPLTG
Sbjct: 360 YLKTRVAPQVPTKVREAFITAVEKGNIRTMPNRSMPADPIPTPGALLLGDAFNMRHPLTG 419

Query: 359 GGMTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYKVFCA 418
           GGMTVAL+DIVVLRDLLRP+ NLND + L  Y+ESFYTLRKPVASTINTLA ALYKVF A
Sbjct: 420 GGMTVALADIVVLRDLLRPIRNLNDKEALSKYIESFYTLRKPVASTINTLADALYKVFLA 479

Query: 419 SPDPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRLLIPF 478
           S D AR EMREACFDYLSLGGVFSSGPVALLSGLNPRPLSL  HFFAVAIY V RL++PF
Sbjct: 480 SSDEARTEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLVLHFFAVAIYAVCRLMLPF 539

Query: 479 PSPKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRTPP 525
           PS +  W+G R+I+ A+ IIFPIIK EGVRQMFFP T+PA YR PP
Sbjct: 540 PSIESFWLGARIISSASSIIFPIIKAEGVRQMFFPRTIPAIYRAPP 585

BLAST of Cmc01g0004571 vs. TAIR 10
Match: AT5G24150.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 481.9 bits (1239), Expect = 6.7e-136
Identity = 235/468 (50.21%), Postives = 325/468 (69.44%), Query Frame = 0

Query: 58  DDDHPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELG 117
           +D   D+I+VGAGV G+ALA+ LAKDGR+VHVIERDL EP+RI+GE +QPGG L L +LG
Sbjct: 42  EDGATDVIIVGAGVGGSALAYALAKDGRRVHVIERDLREPERIMGEFMQPGGRLMLSKLG 101

Query: 118 LEDSLNGIDAQQVFGYALYKNGSHTRLAYPLE--KFDSNVSGRSFHNGRFIQRLREKAAA 177
           LED L GIDAQ+  G  +YK+G     ++P++   F  + S RSFHNGRF+QRLR+KA++
Sbjct: 102 LEDCLEGIDAQKATGMTVYKDGKEAVASFPVDNNNFPFDPSARSFHNGRFVQRLRQKASS 161

Query: 178 LSNVRMEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKPQI 237
           L NVR+E+GTV S++EE G++KGV YK   G+E TA APLT+VCDG +SNLRR L     
Sbjct: 162 LPNVRLEEGTVKSLIEEKGVIKGVTYKNSAGEETTALAPLTVVCDGCYSNLRRSLNDNNA 221

Query: 238 DIPSCFVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGE 297
           ++ S  VG + +NC+L       ++++ PS  +LY ISST+VRC+ ++    +PS++NGE
Sbjct: 222 EVLSYQVGFISKNCQLEEPEKLKLIMSKPSFTMLYQISSTDVRCVFEVLPNNIPSISNGE 281

Query: 298 MAHYLKTAVAPQLPPEVYPAFIAAIDKG-NIKSTTNRSMPAAPQSTPGALLLGDAFNMRH 357
           MA ++K  +APQ+P ++   F+  ID+G +IK+   + M A      G +LLGDAFNMRH
Sbjct: 282 MATFVKNTIAPQVPLKLRKIFLKGIDEGEHIKAMPTKKMTATLSEKKGVILLGDAFNMRH 341

Query: 358 PLTGGGMTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYK 417
           P    GM V LSDI++LR LL+PLSNL +A  +   ++SFY +RKP+++T+NTL  A  +
Sbjct: 342 PAIASGMMVLLSDILILRRLLQPLSNLGNAQKISQVIKSFYDIRKPMSATVNTLGNAFSQ 401

Query: 418 VFCASPDPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRL 477
           V  AS D A++ MR+ C+DYLS GG  +SG +ALL G+NPRP+SL  H  A+ +  +G L
Sbjct: 402 VLVASTDEAKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIGHL 461

Query: 478 LIPFPSPKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRT 523
           L PFPSP RIW  +RL   A  ++ P +K EGV QM FP    AY ++
Sbjct: 462 LSPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509

BLAST of Cmc01g0004571 vs. TAIR 10
Match: AT5G24160.1 (squalene monoxygenase 6 )

HSP 1 Score: 471.9 bits (1213), Expect = 6.9e-133
Identity = 233/468 (49.79%), Postives = 322/468 (68.80%), Query Frame = 0

Query: 59  DDHPDLIVVGAGVAGAALAFTLAKDGRKVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 118
           D   D+I+VGAGV G+ALA+ LAKDGR+VHVIERD+ EP+R++GE +QPGG L L +LGL
Sbjct: 43  DGAADVIIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGEFMQPGGRLMLSKLGL 102

Query: 119 EDSLNGIDAQQVFGYALYKNGSHTRLAYPLE--KFDSNVSGRSFHNGRFIQRLREKAAAL 178
           +D L  IDAQ+  G A+YK+G      +P++   F    S RSFHNGRF+Q+LR KA +L
Sbjct: 103 QDCLEDIDAQKATGLAVYKDGKEADAPFPVDNNNFSYEPSARSFHNGRFVQQLRRKAFSL 162

Query: 179 SNVRMEQGTVTSIVEEDGIVKGVQYKTKNGKELTAYAPLTIVCDGGFSNLRRKLCKP-QI 238
           SNVR+E+GTV S++EE G+VKGV YK K G+E TA APLT+VCDG +SNLRR L      
Sbjct: 163 SNVRLEEGTVKSLLEEKGVVKGVTYKNKEGEETTALAPLTVVCDGCYSNLRRSLNDDNNA 222

Query: 239 DIPSCFVGLVLENCKLPLENHGHVVLADPSPILLYPISSTEVRCLVDIPGQKVPSVANGE 298
           +I S  VG + +NC+L      H++L+ PS  ++Y ISST+VRC  ++  +  PS+ANGE
Sbjct: 223 EIMSYIVGYISKNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCGFEVLPENFPSIANGE 282

Query: 299 MAHYLKTAVAPQLPPEVYPAFIAAIDKG-NIKSTTNRSMPAAPQSTPGALLLGDAFNMRH 358
           M+ ++K  + PQ+PP++   F+  ID+G +IK    + M +      G ++LGDAFNMRH
Sbjct: 283 MSTFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDAFNMRH 342

Query: 359 PLTGGGMTVALSDIVVLRDLLRPLSNLNDADVLCNYLESFYTLRKPVASTINTLAGALYK 418
           P+   GM V LSDI++LR LL+PLSNL DA+ +   + SFY +RKP+++T+NTL  A  +
Sbjct: 343 PVVASGMMVLLSDILILRRLLQPLSNLGDANKVSEVINSFYDIRKPMSATVNTLGNAFSQ 402

Query: 419 VFCASPDPARKEMREACFDYLSLGGVFSSGPVALLSGLNPRPLSLFCHFFAVAIYGVGRL 478
           V   S D A++ MR+  +DYL  GG  +SG +ALL G+NPRPLSL  H  A+ +  +G+L
Sbjct: 403 VLIGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSIGQL 462

Query: 479 LIPFPSPKRIWIGVRLITGAAGIIFPIIKDEGVRQMFFPATVPAYYRT 523
           L PFPSP RIW  ++L   A  ++ P +K EGV QM FPA   AY+++
Sbjct: 463 LSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0031396.11.5e-30299.24squalene monooxygenase-like [Cucumis melo var. makuwa] >TYK06847.1 squalene mono... [more]
XP_004136919.11.2e-28895.44squalene monooxygenase SE2 [Cucumis sativus] >KGN43821.1 hypothetical protein Cs... [more]
XP_038887697.14.5e-27591.44squalene monooxygenase SE2-like [Benincasa hispida][more]
XP_023553953.11.5e-26287.05squalene epoxidase 1-like isoform X1 [Cucurbita pepo subsp. pepo] >AXK50228.1 sq... [more]
XP_022952179.19.6e-26286.67squalene epoxidase 1-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
B7TWW51.4e-22680.60Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2[more]
O486517.0e-22379.27Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1[more]
Q8VYH21.7e-21677.71Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1[more]
Q9SM024.9e-21672.37Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1[more]
O810001.8e-21075.54Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1... [more]
Match NameE-valueIdentityDescription
A0A5A7SMZ27.1e-30399.24Squalene monooxygenase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1... [more]
A0A0A0K7N05.8e-28995.44Squalene monooxygenase OS=Cucumis sativus OX=3659 GN=Csa_7G069710 PE=3 SV=1[more]
A0A345Z0U97.2e-26387.05Squalene monooxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1[more]
A0A6J1GKV94.7e-26286.67Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111454931 PE=3 SV=1[more]
A0A6J1I4X32.6e-26086.48Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111471032 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G37760.11.2e-21777.71squalene epoxidase 3 [more]
AT1G58440.13.5e-21772.37FAD/NAD(P)-binding oxidoreductase family protein [more]
AT2G22830.11.3e-21175.54squalene epoxidase 2 [more]
AT5G24150.16.7e-13650.21FAD/NAD(P)-binding oxidoreductase family protein [more]
AT5G24160.16.9e-13349.79squalene monoxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Melon (Charmono) v1.1
Date Performed: 2022-10-13
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00420RNGMNOXGNASEcoord: 212..227
score: 32.71
coord: 340..355
score: 30.42
coord: 63..85
score: 37.97
coord: 355..371
score: 32.55
NoneNo IPR availablePFAMPF13450NAD_binding_8coord: 66..92
e-value: 3.8E-5
score: 23.8
NoneNo IPR availablePANTHERPTHR10835:SF17SQUALENE MONOOXYGENASE-RELATEDcoord: 9..525
IPR036188FAD/NAD(P)-binding domain superfamilyGENE3D3.50.50.60coord: 56..434
e-value: 4.1E-34
score: 120.0
IPR036188FAD/NAD(P)-binding domain superfamilySUPERFAMILY51905FAD/NAD(P)-binding domaincoord: 62..437
IPR013698Squalene epoxidasePFAMPF08491SEcoord: 213..485
e-value: 7.3E-118
score: 392.6
IPR040125Squalene monooxygenasePANTHERPTHR10835SQUALENE MONOOXYGENASEcoord: 9..525

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Cmc01g0004571.1Cmc01g0004571.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016126 sterol biosynthetic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0004506 squalene monooxygenase activity