CmaCh17G010180 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh17G010180
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	nifU-like protein 1, chloroplastic
Location	Cma_Chr17: 7396472 .. 7398685 (-)
RNA-Seq Expression	CmaCh17G010180
Synteny	CmaCh17G010180

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGTATAAAACTTGGATCGGCGGACGTAAAACAAGTTCGTCATCATCGGCTAGATTCATTTTCATTTCTTCTTCATCAATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCGCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCACTGAATAGGGCGTTGCCCAGACAATCCATTAGAGCTTCCGCTTCGAATTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTTGAACTGACCATTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGACGTCGTGTCCGTTGAAGATGGAATCGTCTCTCTCAAACTCGTTGGTGGGTGAATTTCTGATCTCATTTCATTGAATGGGTTTGTTTTAATCTTGTTTGTTAATTTAGCTTTGTGTATTGAAATTTTTGTAATTGAATGATTGATTGCTATTGCTTATGTGCGCGAAATGTTGATGAACAATCGAAAAGGTTAGGGGTTTATGCCTCAGAAAATTCTAAATTCTTCATTTGGGTGTTTCTTAGACCAAGAGTTATAGGATCCTTATAAGTTGAAAACATCAAAGAACTCTTAGTTCCATGGCCTTGGTACTTAGAAGCTCAAATACCACTGAAAAGGTTCATGAAGATGCAATATCCTTTTGCCGTCCAGGTTCACAATTCTTATATGTTTAGGTTTTCATCATATTTTTTGCTCTCTGCCCCATTGCTAAGAGTTTTCAGGAGAATTTTGTTGGGAGTTCTGGTATGTTTGGAATATGGATGTTTCCTAGGACATGTGGGATTTTCATCCGGTCTAAACTCACGGACTGAAGATTGATGTAGCTCTTTCGCTTGTAACAACCCAAGCTCACCGCTAACAAATATTGTCCACTTTGGTCCGTTGCATATTGCCATCCGCTTCATGGTTTTGAAACGTGTCTGTTAAGGATAAGTTTTCTCACCCTTTTAAGGAGCGCTTTGTTCTTCTCTCCAACCGATGTGGGATCTCACAATTCACCCCCTTGGGAGCCCAGTATCCTCGTTAGCATACCGCCTGGTGTTTGGCTCGGATGCCATTTGTAACAGCCCAAACCCACCGCTAACATATATTGTCCGCTTTAGCCCGTTACGTATCGCTGTCAGCCTCACAGTTCTAAAACGTGTCTGCTAGGGAGAGGTTTTCATACCCATATATATAAGGAATGTTTCGTTCCCCTCTCCAACCGAGTGGGATCTCACACAACTAGTTTTAAAACTTTTCCTTATTGACTAGTTGAAGAGCATTTATTTGTTATCTCGGTCCAGGCTGGGTTTGGACCTCTAGTACCTGCTCGACACGTGCCTGGTGAGTTTATCTGTTTCGTGTTCTGTAGGGGAAAATGACTCCTTGAAGTAGGTGGGCAAAAGGTAGTGAGCAGATAATCCTCACTATCCTGTTAGACATCTTGCAACCGATACCTGAGTTTATCTTTGAGTTTTGAGCTTAGATTTCATTTATTCCATCAGCTTCAAGATTTTACACTTTTTTTTTTAACTAAAGACTTAGCAAGAATCAAATCGATCCTCAGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGTGAGTTTTCTCTGGACGAACAAATACAAAAGAACTCGAACAAGTTTCGATGTTTTGTGACACTTGCTGATGTTAAATCCAAACAAAAACAGAGAGCTGAATTAGTATTAACGTTGGATGTTGCACCAATGCAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTATGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAGCCTTTTCTCAAGTTCATTGAACAGAGTAGCCATGTAAAGTTCTATTGTTCAATAAATGATACATATATTATTGATGACTAACTTATGCCAATGATATTGCCTGTTTTAGCTCGTTAA

mRNA sequence

AGTATAAAACTTGGATCGGCGGACGTAAAACAAGTTCGTCATCATCGGCTAGATTCATTTTCATTTCTTCTTCATCAATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCGCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCACTGAATAGGGCGTTGCCCAGACAATCCATTAGAGCTTCCGCTTCGAATTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTTGAACTGACCATTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGACGTCGTGTCCGTTGAAGATGGAATCGTCTCTCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTATGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAGCCTTTTCTCAAGTTCATTGAACAGAGTAGCCATGTAAAGTTCTATTGTTCAATAAATGATACATATATTATTGATGACTAACTTATGCCAATGATATTGCCTGTTTTAGCTCGTTAA

Coding sequence (CDS)

ATGGCGTCTTTAACTATCTCCGGCCTCCTCAAAACCCCTCTTTATTATTCGCAAGCTCTTGCAAATCACCGCCAGTACCCACCTTCTGCTTTTGGGCAACATCAAATACCCGTTTCACTGAATAGGGCGTTGCCCAGACAATCCATTAGAGCTTCCGCTTCGAATTCTGGTCCATCCACGAGCTCCTCGCCAGGGCTGTACTCCGCCCAGAAATTTGAACTGACCATTGCAAACGTAGACTTGGTTCTTGAGGACGTTAGGCCCTATCTAATCGCAGATGGAGGAAATGTCGACGTCGTGTCCGTTGAAGATGGAATCGTCTCTCTCAAACTCGTTGGAGCATGCGGTAGCTGCCCGAGCTCAACAACCACCATGAAGATGGGAATCGAACGAGTACTGAAGGAAAAGTTTGGAGATTCAGTGAAGGAAATATGCCAAGTATATGATGAAGAGCCAAAGGAAATAACAACCGAGGCAGTAAATAGTCATCTTGACATATTGAGGCCAGCCATAAGGAACTATGGCGGGAGCGTGGAAGTAATATCGATCGATGGTGGCAATTGCGTCGTTATGTATGAGGGTCCTGAATCTATTGGTACAGGAATTAAAGCAGCCATTAAGGAGAAGTTCCCAGATATTACAAACGTTGTGTTTTCAAGCTAG

Protein sequence

MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPSTSSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS

Homology

[Close]

BLAST of CmaCh17G010180 vs. ExPASy Swiss-Prot
Match: Q93W77 (NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 4.7e-64
Identity = 129/193 (66.84%), Postives = 154/193 (79.79%), Query Frame = 0


Query: 31  FGQHQIPVSLNRALPRQSIRASASN---SGPSTSSSPGLYSAQKFELTIANVDLVLEDVR 90
           F   +  +S      R +I  S+     S  ++  S GLYSAQ F+LT  NVDLVLEDVR
Sbjct: 39  FANKRRHISRTAIFHRSAISGSSQGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVR 98

Query: 91  PYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQV 150
           P+LI+DGGNVDVVSVEDG+VSLKL GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV
Sbjct: 99  PFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQV 158

Query: 151 YDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVMYEGPESIGTGIKAAIK 210
           +DEE K+IT EAVN+HLDILRPAI+NYGGSVEV+S++G +CVV Y GPESIG GI+AAIK
Sbjct: 159 FDEEVKQITVEAVNAHLDILRPAIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIK 218

Query: 211 EKFPDITNVVFSS 221
           EKF DI+NV F+S
Sbjct: 219 EKFKDISNVTFTS 231

[Close]

BLAST of CmaCh17G010180 vs. ExPASy Swiss-Prot
Match: Q93W20 (NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1 SV=1)

HSP 1 Score: 100.1 bits (248), Expect = 3.2e-20
Identity = 58/148 (39.19%), Postives = 85/148 (57.43%), Query Frame = 0


Query: 74  LTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRP-AIRNYGGSVEVISIDGGNCVVMY 193
            EK  + V       +E   E+  E +   L+ +RP  I    GS++++ I+     +  
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGIKAAIKEKFPDITNV 217
            GP     ++   +   ++EK P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

[Close]

BLAST of CmaCh17G010180 vs. ExPASy Swiss-Prot
Match: Q84LK7 (NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=NIFU1 PE=1 SV=1)

HSP 1 Score: 95.9 bits (237), Expect = 6.1e-19
Identity = 62/152 (40.79%), Postives = 86/152 (56.58%), Query Frame = 0


Query: 68  SAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKM 127
           +A +  LT  NV+ VL+ VRPYL ADGG+V +  +   +V LKL GACGSCPSS  T+K 
Sbjct: 69  TAVQLPLTAGNVESVLDQVRPYLTADGGDVALHEIAGNVVRLKLQGACGSCPSSLITIKR 128

Query: 128 GIERVLKEKFGDSVKEICQVYDEEP-KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDG 187
           GIER L EK  D V  +  V D+E   E+  E V   L+ +RP +    GG ++ + I G
Sbjct: 129 GIERRLMEKIPD-VAAVEPVTDKETGLELNEENVEKVLNEIRPYLAGTGGGGLQFLMIKG 188

Query: 188 GNCVVMYEGPESIGTGIKAAI----KEKFPDI 214
               V   GP ++   ++ A+    +EK P I
Sbjct: 189 PIVKVRLTGPAAVVRTVRIAVSKKLREKIPSI 219

[Close]

BLAST of CmaCh17G010180 vs. ExPASy Swiss-Prot
Match: Q84RQ7 (NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 6.7e-18
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 74  LTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  +V LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDGGNCVV 193
           ++K  + +  + Q  + E    E+  E +   L  LRP +    GG +E++ IDG    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 MYEGPESIGTGIKAAIKEK 210
              GP +    ++ A+ +K
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

[Close]

BLAST of CmaCh17G010180 vs. ExPASy Swiss-Prot
Match: O32119 (Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224308 GN=yutI PE=3 SV=1)

HSP 1 Score: 76.3 bits (186), Expect = 5.0e-13
Identity = 38/70 (54.29%), Postives = 53/70 (75.71%), Query Frame = 0


Query: 79  VDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFG 138
           V  VL+ +RP+L+ DGG+ ++V V++GIV L+L+GACGSCPSST T+K GIER L E+  
Sbjct: 43  VQEVLDKLRPFLLRDGGDCELVDVDEGIVKLRLLGACGSCPSSTITLKAGIERALLEEV- 102

Query: 139 DSVKEICQVY 149
             V E+ QV+
Sbjct: 103 PGVVEVEQVF 111

[Close]

BLAST of CmaCh17G010180 vs. ExPASy TrEMBL
Match: A0A6J1JN01 (nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 5.0e-117
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of CmaCh17G010180 vs. ExPASy TrEMBL
Match: A0A6J1GMI1 (nifU-like protein 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455795 PE=3 SV=1)

HSP 1 Score: 421.4 bits (1082), Expect = 2.3e-114
Identity = 215/220 (97.73%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNR LPRQ+IRASAS+SGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT ANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVV YEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of CmaCh17G010180 vs. ExPASy TrEMBL
Match: A0A1S3BQX1 (nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.6e-102
Identity = 193/220 (87.73%), Postives = 206/220 (93.64%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL RA P +++RAS SNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISI+GGNC+V YEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of CmaCh17G010180 vs. ExPASy TrEMBL
Match: A0A6J1CRB9 (nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111014036 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 3.5e-102
Identity = 195/219 (89.04%), Postives = 203/219 (92.69%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLT SGLLKTP  YSQ  AN+RQYPPSA  QHQ P SL R LPR+ I+ASASNSGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFS 220
           ISIDGG+C+V YEGPESIGTGIKAAIKE+FPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

[Close]

BLAST of CmaCh17G010180 vs. ExPASy TrEMBL
Match: A0A5A7TT28 (NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G001900 PE=3 SV=1)

HSP 1 Score: 380.2 bits (975), Expect = 5.9e-102
Identity = 192/220 (87.27%), Postives = 206/220 (93.64%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL RA P +++RAS SNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISI+GG+C+V YEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGDCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of CmaCh17G010180 vs. NCBI nr
Match: XP_022991837.1 (nifU-like protein 1, chloroplastic [Cucurbita maxima])

HSP 1 Score: 430.3 bits (1105), Expect = 1.0e-116
Identity = 220/220 (100.00%), Postives = 220/220 (100.00%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of CmaCh17G010180 vs. NCBI nr
Match: XP_022953177.1 (nifU-like protein 1, chloroplastic [Cucurbita moschata] >KAG6575747.1 NifU-like protein 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7014298.1 NifU-like protein 1, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 421.4 bits (1082), Expect = 4.8e-114
Identity = 215/220 (97.73%), Postives = 217/220 (98.64%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNR LPRQ+IRASAS+SGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRVLPRQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT ANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVV YEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of CmaCh17G010180 vs. NCBI nr
Match: XP_023547622.1 (nifU-like protein 1, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 415.2 bits (1066), Expect = 3.4e-112
Identity = 212/220 (96.36%), Postives = 215/220 (97.73%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLTISGLLKTPLYYSQALANHRQYPP +FGQHQIPV LNRALP Q+IRASAS+SGPST
Sbjct: 1   MASLTISGLLKTPLYYSQALANHRQYPPFSFGQHQIPVCLNRALPSQAIRASASSSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELT ANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTTANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISIDGGNCVV YEGPESIGTGIKAAIKEKFPDITNVVFSS
Sbjct: 181 ISIDGGNCVVKYEGPESIGTGIKAAIKEKFPDITNVVFSS 220

[Close]

BLAST of CmaCh17G010180 vs. NCBI nr
Match: XP_008451442.1 (PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo])

HSP 1 Score: 382.1 bits (980), Expect = 3.2e-102
Identity = 193/220 (87.73%), Postives = 206/220 (93.64%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLT SGLLKTPL  SQ+ AN+ QYPP A+GQHQ+PVSL RA P +++RAS SNSGPST
Sbjct: 1   MASLTTSGLLKTPLKNSQSPANYFQYPPFAYGQHQLPVSLKRAFPGRAVRASPSNSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVN+HLDILRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNNHLDILRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFSS 221
           ISI+GGNC+V YEGPESIGTG+KAAIKE+FPDITNVVFSS
Sbjct: 181 ISINGGNCLVKYEGPESIGTGVKAAIKERFPDITNVVFSS 220

[Close]

BLAST of CmaCh17G010180 vs. NCBI nr
Match: XP_022144330.1 (nifU-like protein 1, chloroplastic [Momordica charantia])

HSP 1 Score: 380.9 bits (977), Expect = 7.2e-102
Identity = 195/219 (89.04%), Postives = 203/219 (92.69%), Query Frame = 0


Query: 1   MASLTISGLLKTPLYYSQALANHRQYPPSAFGQHQIPVSLNRALPRQSIRASASNSGPST 60
           MASLT SGLLKTP  YSQ  AN+RQYPPSA  QHQ P SL R LPR+ I+ASASNSGPST
Sbjct: 1   MASLTTSGLLKTPPCYSQIPANYRQYPPSAHRQHQGPNSLKRVLPRRGIKASASNSGPST 60

Query: 61  SSSPGLYSAQKFELTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPS 120
           SSSPGLYSAQKFELTI NVDLVLEDVRPYLIADGGNVDVVSVEDG+VSLKLVGACGSCPS
Sbjct: 61  SSSPGLYSAQKFELTIGNVDLVLEDVRPYLIADGGNVDVVSVEDGVVSLKLVGACGSCPS 120

Query: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRPAIRNYGGSVEV 180
           STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKE T EAVNSHLD+LRPAIRNYGGSVEV
Sbjct: 121 STTTMKMGIERVLKEKFGDSVKEICQVYDEEPKETTPEAVNSHLDVLRPAIRNYGGSVEV 180

Query: 181 ISIDGGNCVVMYEGPESIGTGIKAAIKEKFPDITNVVFS 220
           ISIDGG+C+V YEGPESIGTGIKAAIKE+FPDITNVVFS
Sbjct: 181 ISIDGGDCLVKYEGPESIGTGIKAAIKERFPDITNVVFS 219

[Close]

BLAST of CmaCh17G010180 vs. TAIR 10
Match: AT4G01940.1 (NFU domain protein 1 )

HSP 1 Score: 245.7 bits (626), Expect = 3.4e-65
Identity = 129/193 (66.84%), Postives = 154/193 (79.79%), Query Frame = 0


Query: 31  FGQHQIPVSLNRALPRQSIRASASN---SGPSTSSSPGLYSAQKFELTIANVDLVLEDVR 90
           F   +  +S      R +I  S+     S  ++  S GLYSAQ F+LT  NVDLVLEDVR
Sbjct: 39  FANKRRHISRTAIFHRSAISGSSQGEKISPLASGVSSGLYSAQTFDLTPQNVDLVLEDVR 98

Query: 91  PYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVLKEKFGDSVKEICQV 150
           P+LI+DGGNVDVVSVEDG+VSLKL GAC SCPSS+TTM MGIERVLKEKFGD++K+I QV
Sbjct: 99  PFLISDGGNVDVVSVEDGVVSLKLQGACTSCPSSSTTMTMGIERVLKEKFGDALKDIRQV 158

Query: 151 YDEEPKEITTEAVNSHLDILRPAIRNYGGSVEVISIDGGNCVVMYEGPESIGTGIKAAIK 210
           +DEE K+IT EAVN+HLDILRPAI+NYGGSVEV+S++G +CVV Y GPESIG GI+AAIK
Sbjct: 159 FDEEVKQITVEAVNAHLDILRPAIKNYGGSVEVLSVEGEDCVVKYVGPESIGMGIQAAIK 218

Query: 211 EKFPDITNVVFSS 221
           EKF DI+NV F+S
Sbjct: 219 EKFKDISNVTFTS 231

[Close]

BLAST of CmaCh17G010180 vs. TAIR 10
Match: AT5G49940.1 (NIFU-like protein 2 )

HSP 1 Score: 100.1 bits (248), Expect = 2.3e-21
Identity = 58/148 (39.19%), Postives = 85/148 (57.43%), Query Frame = 0


Query: 74  LTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAVNSHLDILRP-AIRNYGGSVEVISIDGGNCVVMY 193
            EK  + V       +E   E+  E +   L+ +RP  I    GS++++ I+     +  
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENIEKVLEEIRPYLIGTADGSLDLVEIEDPIVKIRI 204

Query: 194 EGPE----SIGTGIKAAIKEKFPDITNV 217
            GP     ++   +   ++EK P I  V
Sbjct: 205 TGPAAGVMTVRVAVTQKLREKIPSIAAV 232

[Close]

BLAST of CmaCh17G010180 vs. TAIR 10
Match: AT4G25910.1 (NFU domain protein 3 )

HSP 1 Score: 92.4 bits (228), Expect = 4.8e-19
Identity = 56/139 (40.29%), Postives = 83/139 (59.71%), Query Frame = 0


Query: 74  LTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL++VRP L+ADGGNV +  ++  +V LKL GACGSCPSS+ T+KMGIE  L
Sbjct: 84  LTEENVERVLDEVRPSLMADGGNVALHEIDGLVVVLKLQGACGSCPSSSMTLKMGIESRL 143

Query: 134 KEKFGDSVKEICQVYDEEP--KEITTEAVNSHLDILRPAIRNY-GGSVEVISIDGGNCVV 193
           ++K  + +  + Q  + E    E+  E +   L  LRP +    GG +E++ IDG    V
Sbjct: 144 RDKIPE-IMSVEQFLESETGGLELNDENIEKVLSELRPYLSGTGGGGLELVEIDGYVVKV 203

Query: 194 MYEGPESIGTGIKAAIKEK 210
              GP +    ++ A+ +K
Sbjct: 204 RLTGPAAGVMTVRVALTQK 221

[Close]

BLAST of CmaCh17G010180 vs. TAIR 10
Match: AT5G49940.2 (NIFU-like protein 2 )

HSP 1 Score: 83.6 bits (205), Expect = 2.2e-16
Identity = 44/87 (50.57%), Postives = 58/87 (66.67%), Query Frame = 0


Query: 74  LTIANVDLVLEDVRPYLIADGGNVDVVSVEDGIVSLKLVGACGSCPSSTTTMKMGIERVL 133
           LT  NV+ VL+++RPYL++DGGNV +  ++  IV +KL GACGSCPSST TMKMGIER L
Sbjct: 85  LTEENVESVLDEIRPYLMSDGGNVALHEIDGNIVRVKLQGACGSCPSSTMTMKMGIERRL 144

Query: 134 KEKFGDSVKEICQVYDEEPKEITTEAV 161
            EK  + V       +E   E+  E +
Sbjct: 145 MEKIPEIVAVEALPDEETGLELNEENI 171

[Close]

BLAST of CmaCh17G010180 vs. TAIR 10
Match: AT3G20970.1 (NFU domain protein 4 )

HSP 1 Score: 56.2 bits (134), Expect = 3.8e-08
Identity = 34/85 (40.00%), Postives = 51/85 (60.00%), Query Frame = 0


Query: 75  TIANVDLVLED-VRPYLIADGGNVDVVSV--EDGIVSLKLVGACGSCPSSTTTMKMGIER 134
           T+A +  +LE  +RP +  DGG+++      E GIV L++ GAC  CPSS+ T+K GIE 
Sbjct: 192 TVAMIKELLETRIRPAVQDDGGDIEYCGFDPESGIVKLRMQGACSGCPSSSVTLKSGIEN 251

Query: 135 VLKEKFGDSVKEICQVYDEEPKEIT 157
           +L   +   VK + Q +D E +E T
Sbjct: 252 MLMH-YVSEVKGVEQEFDGEDEEGT 275

The following BLAST results are available for this feature:

BLAST of CmaCh17G010180 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93W77	4.7e-64	66.84	NifU-like protein 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU1 PE=1...	[more]
Q93W20	3.2e-20	39.19	NifU-like protein 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU2 PE=1...	[more]
Q84LK7	6.1e-19	40.79	NifU-like protein 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=N...	[more]
Q84RQ7	6.7e-18	40.29	NifU-like protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NIFU3 PE=2...	[more]
O32119	5.0e-13	54.29	Putative nitrogen fixation protein YutI OS=Bacillus subtilis (strain 168) OX=224...	[more]

BLAST of CmaCh17G010180 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JN01	5.0e-117	100.00	nifU-like protein 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111488364 P...	[more]
A0A6J1GMI1	2.3e-114	97.73	nifU-like protein 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111455795...	[more]
A0A1S3BQX1	1.6e-102	87.73	nifU-like protein 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103492737 PE=3 ...	[more]
A0A6J1CRB9	3.5e-102	89.04	nifU-like protein 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11101403...	[more]
A0A5A7TT28	5.9e-102	87.27	NifU-like protein 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold411G...	[more]

BLAST of CmaCh17G010180 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022991837.1	1.0e-116	100.00	nifU-like protein 1, chloroplastic [Cucurbita maxima]	[more]
XP_022953177.1	4.8e-114	97.73	nifU-like protein 1, chloroplastic [Cucurbita moschata] >KAG6575747.1 NifU-like ...	[more]
XP_023547622.1	3.4e-112	96.36	nifU-like protein 1, chloroplastic [Cucurbita pepo subsp. pepo]	[more]
XP_008451442.1	3.2e-102	87.73	PREDICTED: nifU-like protein 1, chloroplastic [Cucumis melo]	[more]
XP_022144330.1	7.2e-102	89.04	nifU-like protein 1, chloroplastic [Momordica charantia]	[more]

BLAST of CmaCh17G010180 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G01940.1	3.4e-65	66.84	NFU domain protein 1	[more]
AT5G49940.1	2.3e-21	39.19	NIFU-like protein 2	[more]
AT4G25910.1	4.8e-19	40.29	NFU domain protein 3	[more]
AT5G49940.2	2.2e-16	50.57	NIFU-like protein 2	[more]
AT3G20970.1	3.8e-08	40.00	NFU domain protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001075	NIF system FeS cluster assembly, NifU, C-terminal	PFAM	PF01106	NifU	coord: 162..217 e-value: 1.9E-4 score: 21.5 coord: 80..139 e-value: 1.2E-22 score: 79.8
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 71..154 e-value: 8.2E-27 score: 95.1
IPR034904	Fe-S cluster assembly domain superfamily	GENE3D	3.30.300.130		coord: 155..218 e-value: 9.3E-11 score: 43.7
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 157..217
IPR034904	Fe-S cluster assembly domain superfamily	SUPERFAMILY	117916	Fe-S cluster assembly (FSCA) domain-like	coord: 76..149
None	No IPR available	PANTHER	PTHR11178	IRON-SULFUR CLUSTER SCAFFOLD PROTEIN NFU-RELATED	coord: 40..219
None	No IPR available	PANTHER	PTHR11178:SF15	NIFU-LIKE PROTEIN 1, CHLOROPLASTIC	coord: 40..219

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh17G010180.1	CmaCh17G010180.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016226	iron-sulfur cluster assembly
biological_process	GO:0097428	protein maturation by iron-sulfur cluster transfer
cellular_component	GO:0009507	chloroplast
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0005506	iron ion binding
molecular_function	GO:0051536	iron-sulfur cluster binding