CmaCh14G009500 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh14G009500
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	GDSL esterase/lipase
Location	Cma_Chr14: 4920375 .. 4922677 (+)
RNA-Seq Expression	CmaCh14G009500
Synteny	CmaCh14G009500

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GTGTGGGTAAGAACAAAAAAGATGACCAAATCAAGATCAAGAACAACAACATTAATGTTTGATCCAAAGCATAATCCATTTTGGGTTTGTGTAATTTCTCTTGCCATATTCATTCCATCAACAAGCTGCACCCCTACACCGCCAACTTTGATCAACTTCGGCGACTCGAACTCTGATACCGGTGGTGTTCTTGCTGGGACCGGACTCCCCATTGGCCTCCCCCACGGCATTACCTTCTTCCATCATGGTACCGGGCGCTTCGGTGACGGTCGCCTCATAATTGACTTCTTCTGTGAGTTCCATGCTATTTAGTAGGGTTTGTTTCAGATTAGGCAGTCAAATGTTTGATGTTTGATGTTTGATGTTTTGGGGTTTTGGGGTTCAGGTGAAGACTTGAAACTGAGGTATTTGAGTCCATATTTGGAGTCCTTAGCTCCAAATTTTACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCAACTACACTTCCACAGTTCGTTCCTTTTGCTCTTGATGTTCAAATTCGCCAATTCATTCACTTCAAAAACCGTTCTCTTCACCTTCAATCATTAGGTATATGTTTTTCCTTTCTATTGTAATTTCTCCCTCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTNGAGAATAACGGAACATTCTTTATGAGGGTGTGAAAATCTCTCCGTAGCATACGCGTTTTAAAAACATTGAGGAGAAGTCCGAAAGGGAAAGCCAAATAGGACAATATTTGCTAGCAGTAGGCTTGAATTGTTACAAATGGTATTAAAGCCCGACATTGGGCAGTGTGCCAACAAGGACCCTGAGCCCTAAATGGGGTGGATTATGAGATTCCACATTGATTAGAGAGGAGAACGAGTGCCAGCGAAGACGCTGGGCCCCGAAGAAGGGTGGATTGTGGATCCCACATCAGTTCAAGAGGAGAACGAAATATTCTTTATACGAGTGTGGAAACCTCTCCCTAGTCAACGTGTTTTAAAAACCTTAAGGAGAACCATAGAATGGAAAGCCTAAAGAGAACAATATCAGCTAGTAGTAGGCTTGGGTTGTTACCAATGGTATCAGAACTAAACATCGAGCGGTGTGTCAGCAAGAATGCTGAGCCTCGAAGGGGGTGGATTGAGAGATCCCACATCGATTTTTTTATAAGGGTGTGAGAAACCTTTCCCTAACAAACATGTTTTTAAAAATCTTGAGAGGAAATTCAAAAGATAAAGCGGGAAGAGAACAATATCTATTAGCAGTGGGATGTGGGCTTAACAGTTACAATAACAATTTCGAATCCCTACTTCTACGACCTATAAATTTCTTTGTTTGGTTATCAAGATTGTAATGTAGATGGGTTTGTGGCAGGTAAAACTAAGAACATAATGGATGAAGAAGGGTTTAAGAATGGAGTATACATGATTGACATTGGTCAGAATGATCTCTTAGTTGCCCTTTATGCATCAAATTTAACCTACAAACCAGTGGCCCAAAAGATCCCATCTTTTCTTGCAGAAATCAAGCTAGCCATTCAGGTGAACCTTCTTCCACTTTTCCCTTCCAAATTCACCACTCTTAAAACCATTAATAATACACCAAGTTGCAGTGTCTTTGAACTATACACCACTTTATGGCAAGGAATCTTTTGGATCATGTTACGTATTTTAATATTTGAAAACACAACTTACAACATTGTGTACAGAATCTATATGCAAATGGTGGAAGAAAATTTTGGATACACAACACAGGGCCATTAGGTTGCTCTCCAAAAGAGCTGGCATTGCATCCCCACACCCACAAGGATGTTGACCAAATTGGGTGCCTTAGGGTTCACAATCAAGTCGCCAAATCCTTCAATAAAGACCTAAAAAATGTGTGCAAACAGCTACGATCTCAATTTAAGGATGCCATCGTTGTCTACGTCGACGTATACACCATCAAATATAATCTCTTCGTCCATCCCGACGTATATGGTATGAAATCTCGAACCAAACTATAAATAAGTTTAAAGACTAATTAAGAGAGAATGTTTTGGTTTTTGTGAAAGGGTTTGAGAAGGCATTAATGGCATGCTGTGGGTACGGAGGGGCGCCAAATAATTACAACGTGAAGGCAACATGTGGGCAACCGGGGTACTCCATCTGCGCCAACCCTTTAAGATCTATCGTTTGTGTGAATGTTTGATTAATCCACCACACCTAAATGGTGTGAAAGTTATCTTATTTATAATCAAATAAATTACAAGGATATGGAAATAGTAATAAATGGAAATAACAATAAAT

mRNA sequence

GTGTGGGTAAGAACAAAAAAGATGACCAAATCAAGATCAAGAACAACAACATTAATGTTTGATCCAAAGCATAATCCATTTTGGGTTTGTGTAATTTCTCTTGCCATATTCATTCCATCAACAAGCTGCACCCCTACACCGCCAACTTTGATCAACTTCGGCGACTCGAACTCTGATACCGGTGGTGTTCTTGCTGGGACCGGACTCCCCATTGGCCTCCCCCACGGCATTACCTTCTTCCATCATGGTACCGGGCGCTTCGGTGACGGTCGCCTCATAATTGACTTCTTCTGTGAAGACTTGAAACTGAGGTATTTGAGTCCATATTTGGAGTCCTTAGCTCCAAATTTTACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCAACTACACTTCCACAGTTCGTTCCTTTTGCTCTTGATGTTCAAATTCGCCAATTCATTCACTTCAAAAACCGTTCTCTTCACCTTCAATCATTAGGTAAAACTAAGAACATAATGGATGAAGAAGGGTTTAAGAATGGAGTATACATGATTGACATTGGTCAGAATGATCTCTTAGTTGCCCTTTATGCATCAAATTTAACCTACAAACCAGTGGCCCAAAAGATCCCATCTTTTCTTGCAGAAATCAAGCTAGCCATTCAGAATCTATATGCAAATGGTGGAAGAAAATTTTGGATACACAACACAGGGCCATTAGGTTGCTCTCCAAAAGAGCTGGCATTGCATCCCCACACCCACAAGGATGTTGACCAAATTGGGTGCCTTAGGGTTCACAATCAAGTCGCCAAATCCTTCAATAAAGACCTAAAAAATGTGTGCAAACAGCTACGATCTCAATTTAAGGATGCCATCGTTGTCTACGTCGACGTATACACCATCAAATATAATCTCTTCGTCCATCCCGACGTATATGGGTTTGAGAAGGCATTAATGGCATGCTGTGGGTACGGAGGGGCGCCAAATAATTACAACGTGAAGGCAACATGTGGGCAACCGGGGTACTCCATCTGCGCCAACCCTTTAAGATCTATCGTTTGTGTGAATGTTTGATTAATCCACCACACCTAAATGGTGTGAAAGTTATCTTATTTATAATCAAATAAATTACAAGGATATGGAAATAGTAATAAATGGAAATAACAATAAAT

Coding sequence (CDS)

ATGACCAAATCAAGATCAAGAACAACAACATTAATGTTTGATCCAAAGCATAATCCATTTTGGGTTTGTGTAATTTCTCTTGCCATATTCATTCCATCAACAAGCTGCACCCCTACACCGCCAACTTTGATCAACTTCGGCGACTCGAACTCTGATACCGGTGGTGTTCTTGCTGGGACCGGACTCCCCATTGGCCTCCCCCACGGCATTACCTTCTTCCATCATGGTACCGGGCGCTTCGGTGACGGTCGCCTCATAATTGACTTCTTCTGTGAAGACTTGAAACTGAGGTATTTGAGTCCATATTTGGAGTCCTTAGCTCCAAATTTTACAAGTGGAGTGAACTTTGCAGTGAGTGGAGCAACTACACTTCCACAGTTCGTTCCTTTTGCTCTTGATGTTCAAATTCGCCAATTCATTCACTTCAAAAACCGTTCTCTTCACCTTCAATCATTAGGTAAAACTAAGAACATAATGGATGAAGAAGGGTTTAAGAATGGAGTATACATGATTGACATTGGTCAGAATGATCTCTTAGTTGCCCTTTATGCATCAAATTTAACCTACAAACCAGTGGCCCAAAAGATCCCATCTTTTCTTGCAGAAATCAAGCTAGCCATTCAGAATCTATATGCAAATGGTGGAAGAAAATTTTGGATACACAACACAGGGCCATTAGGTTGCTCTCCAAAAGAGCTGGCATTGCATCCCCACACCCACAAGGATGTTGACCAAATTGGGTGCCTTAGGGTTCACAATCAAGTCGCCAAATCCTTCAATAAAGACCTAAAAAATGTGTGCAAACAGCTACGATCTCAATTTAAGGATGCCATCGTTGTCTACGTCGACGTATACACCATCAAATATAATCTCTTCGTCCATCCCGACGTATATGGGTTTGAGAAGGCATTAATGGCATGCTGTGGGTACGGAGGGGCGCCAAATAATTACAACGTGAAGGCAACATGTGGGCAACCGGGGTACTCCATCTGCGCCAACCCTTTAAGATCTATCGTTTGTGTGAATGTTTGA

Protein sequence

MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIVCVNV

Homology

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BLAST of CmaCh14G009500 vs. ExPASy Swiss-Prot
Match: Q6NLP7 (GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.6e-122
Identity = 213/320 (66.56%), Postives = 253/320 (79.06%), Query Frame = 0


Query: 24  VISLAIFIPSTSCTP----TPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGR 83
           V+ L++ + S S T       P LINFGDSNSDTGGVLAG GLPIGLPHGITFFH GTGR
Sbjct: 14  VLCLSLLVCSNSETSYKSNKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGR 73

Query: 84  FGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQF 143
            GDGRLI+DF+CE LK+ YLSPYL+SL+PNF  GVNFAVSGAT LP F  F L +QIRQF
Sbjct: 74  LGDGRLIVDFYCEHLKMTYLSPYLDSLSPNFKRGVNFAVSGATALPIF-SFPLAIQIRQF 133

Query: 144 IHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSF 203
           +HFKNRS  L S G+ ++++D+ GF+N +YMIDIGQNDLL+ALY SNLTY PV +KIPS 
Sbjct: 134 VHFKNRSQELISSGR-RDLIDDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEKIPSM 193

Query: 204 LAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSF 263
           L EIK AIQ +Y  GGRKFW+HNTGPLGC+PKELA+H H   D+D IGC RVHN+VAK+F
Sbjct: 194 LLEIKKAIQTVYLYGGRKFWVHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEVAKAF 253

Query: 264 NKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGYGGAPNNYNV 323
           NK L ++C +LRSQFKDA +VYVD+Y+IKY L     +YGF   LMACCGYGG PNNY+ 
Sbjct: 254 NKGLLSLCNELRSQFKDATLVYVDIYSIKYKLSADFKLYGFVDPLMACCGYGGRPNNYDR 313

Query: 324 KATCGQPGYSICANPLRSIV 340
           KATCGQPG +IC +  ++IV
Sbjct: 314 KATCGQPGSTICRDVTKAIV 331

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BLAST of CmaCh14G009500 vs. ExPASy Swiss-Prot
Match: Q9FXB6 (GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.3e-89
Identity = 163/338 (48.22%), Postives = 224/338 (66.27%), Query Frame = 0


Query: 9   TTLMFDPKHNPFWVCVISLAIFI---PS--TSCTPTPPTLINFGDSNSDTGGVLAGTGLP 68
           T  ++    + F++ ++SLA+ I   PS   SCT   P + NFGDSNSDTGG++AG G P
Sbjct: 3   TLFLYSNTFSFFFITLVSLALLILRQPSRAASCT-ARPVIFNFGDSNSDTGGLVAGLGYP 62

Query: 69  IGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGAT 128
           IG P+G  FF   TGR  DGRL+IDF C+ L    L PYL+SL    F +G NFA++G+ 
Sbjct: 63  IGFPNGRLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSP 122

Query: 129 TLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKN--IMDEEGFKNGVYMIDIGQNDLLV 188
           TLP+ VPF+L++Q++QF HFK+RSL L S   +     +   GFKN +YMIDIGQND + 
Sbjct: 123 TLPKNVPFSLNIQVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQND-IA 182

Query: 189 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 248
             +A   +Y    + IP  + EIK +I+ LY  GGR+FWIHNTGPLGC P++L++     
Sbjct: 183 RSFARGNSYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKS 242

Query: 249 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 308
           KD+DQ GCL  +N  A  FN+ L ++C++LR++ +DA ++Y+D+Y IKY+L  + + YGF
Sbjct: 243 KDLDQHGCLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGF 302

Query: 309 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSI 339
           +  LMACCGYGG P NYNVK TCG  G ++C    R I
Sbjct: 303 KSPLMACCGYGGTPYNYNVKITCGHKGSNVCEEGSRFI 336

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BLAST of CmaCh14G009500 vs. ExPASy Swiss-Prot
Match: O80522 (GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2 SV=1)

HSP 1 Score: 330.9 bits (847), Expect = 1.7e-89
Identity = 158/302 (52.32%), Postives = 215/302 (71.19%), Query Frame = 0


Query: 40  PPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYL 99
           PP + NFGDSNSDTGG++AG G  IGLP+G +FF   TGR  DGRL+IDF C+ L    L
Sbjct: 35  PPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRSTGRLSDGRLVIDFLCQSLNTSLL 94

Query: 100 SPYLESL-APNFTSGVNFAVSGATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGK--TK 159
           +PYL+SL    F +G NFA+ G++TLP++VPFAL++Q+ QF+HFK+R+L L S+     +
Sbjct: 95  NPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFALNIQLMQFLHFKSRALELASISDPLKE 154

Query: 160 NIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGR 219
            ++ E GF+N +YMIDIGQND+  + ++  L+Y  V + IP+ ++EIK AI+ LY  GGR
Sbjct: 155 MMIGESGFRNALYMIDIGQNDIADS-FSKGLSYSRVVKLIPNVISEIKSAIKILYDEGGR 214

Query: 220 KFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKD 279
           KFW+HNTGPLGC P++L++  H+ K  D+ GCL  +N  AK FN+ L ++C+ LR++ K+
Sbjct: 215 KFWVHNTGPLGCLPQKLSM-VHS-KGFDKHGCLATYNAAAKLFNEGLDHMCRDLRTELKE 274

Query: 280 AIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPLR 339
           A +VYVD+Y IKY+L  + + YGFEK LMACCGYGG P NYNV  TCG  G   C    R
Sbjct: 275 ANIVYVDIYAIKYDLIANSNNYGFEKPLMACCGYGGPPYNYNVNITCGNGGSKSCDEGSR 333

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BLAST of CmaCh14G009500 vs. ExPASy Swiss-Prot
Match: Q3ECP6 (GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2 SV=1)

HSP 1 Score: 253.4 bits (646), Expect = 3.5e-66
Identity = 144/342 (42.11%), Postives = 194/342 (56.73%), Query Frame = 0


Query: 20  FWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGR 79
           F+V +  ++    S S     P+  NFGDSNSDTG ++AG G+ + LP+G   F   + R
Sbjct: 9   FYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 68

Query: 80  FGDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPFALDV 139
           F DGRL+IDF  +++ L +L+PYL+SL  PNF  G NFA +G+T LP       PF+ D+
Sbjct: 69  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 128

Query: 140 QIRQFIHFKNRSLHLQSLGKT-----KNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTY 199
           QI QFI FK+R++ L  L KT     K +   + +  G+YMIDIGQND+  A Y+   T 
Sbjct: 129 QISQFIRFKSRAIEL--LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK--TL 188

Query: 200 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 259
             V   IPS L   +  ++ LY  GGR  WIHNTGPLGC  + +A        +D+ GC+
Sbjct: 189 DQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCV 248

Query: 260 RVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNL----------FVHP---- 319
             HNQ AK FN  L  +  + ++Q+ DA V YVD+++IK NL          F  P    
Sbjct: 249 SSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGKHFTKPLIDL 308

Query: 320 ------------DVYGFEKALMACCGYGGAPNNYNVKATCGQ 326
                       +V GFEK LMACCG GGAP NY+ + TCGQ
Sbjct: 309 NHLENVGYNKILNVLGFEKPLMACCGVGGAPLNYDSRITCGQ 346

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BLAST of CmaCh14G009500 vs. ExPASy Swiss-Prot
Match: Q9FXE5 (Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1)

HSP 1 Score: 250.4 bits (638), Expect = 3.0e-65
Identity = 133/294 (45.24%), Postives = 179/294 (60.88%), Query Frame = 0


Query: 41  PTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLS 100
           P + NFGDSNSDTGG+ A  G   G PHG +FF    GR+ DGRL+IDF  E L L YLS
Sbjct: 29  PAIFNFGDSNSDTGGLSAAFG-QAGPPHGSSFFGSPAGRYCDGRLVIDFIAESLGLPYLS 88

Query: 101 PYLESLAPNFTSGVNFAVSGA------TTLPQ--FVPFALDVQIRQFIHFKNRSLHLQSL 160
            +L+S+  NF+ G NFA +G+      +TL Q  F PF+LDVQ  QF +F NRS  ++S 
Sbjct: 89  AFLDSVGSNFSHGANFATAGSPIRALNSTLRQSGFSPFSLDVQFVQFYNFHNRSQTVRSR 148

Query: 161 GKTKNIM--DEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFLAEIKLAIQNL 220
           G     M  + + F   +Y  DIGQND L A Y +N T + V  ++P  +++   AI+N+
Sbjct: 149 GGVYKTMLPESDSFSKALYTFDIGQND-LTAGYFANKTVEQVETEVPEIISQFMNAIKNI 208

Query: 221 YANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKQL 280
           Y  GGR FWIHNTGP+GC    +   P+   D D  GC+   N +A+ FN  LK    +L
Sbjct: 209 YGQGGRYFWIHNTGPIGCLAYVIERFPNKASDFDSHGCVSPLNHLAQQFNHALKQAVIEL 268

Query: 281 RSQFKDAIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGYGGAPNNYNVKATCG 325
           RS   +A + YVDVY++K+ LFVH   +GF+ +L++CCG+GG   NYN    CG
Sbjct: 269 RSSLSEAAITYVDVYSLKHELFVHAQGHGFKGSLVSCCGHGG-KYNYNKGIGCG 319

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BLAST of CmaCh14G009500 vs. ExPASy TrEMBL
Match: A0A6J1J5S7 (GDSL esterase/lipase At3g62280-like OS=Cucurbita maxima OX=3661 GN=LOC111482013 PE=3 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.5e-200
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0


Query: 1   MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60
           MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT
Sbjct: 12  MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 71

Query: 61  GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120
           GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG
Sbjct: 72  GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 131

Query: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180
           ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV
Sbjct: 132 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 191

Query: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240
           ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH
Sbjct: 192 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 251

Query: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 300
           KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF
Sbjct: 252 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 311

Query: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 340
           EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV
Sbjct: 312 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 350

[Close]

BLAST of CmaCh14G009500 vs. ExPASy TrEMBL
Match: A0A6J1FA19 (GDSL esterase/lipase At3g62280-like OS=Cucurbita moschata OX=3662 GN=LOC111442195 PE=3 SV=1)

HSP 1 Score: 696.0 bits (1795), Expect = 7.6e-197
Identity = 332/339 (97.94%), Postives = 335/339 (98.82%), Query Frame = 0


Query: 1   MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60
           MTKSRSRTTTLMFDP+HNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT
Sbjct: 1   MTKSRSRTTTLMFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60

Query: 61  GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120
           GLPIGLPHGITFFH GTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG
Sbjct: 61  GLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120

Query: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180
           ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV
Sbjct: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180

Query: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240
           ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH
Sbjct: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240

Query: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 300
           KDVDQIGCLRVHNQVAKSFNKDLKNVCK LRSQFKDAIVVYVDVYTIKYNLF HPDVYGF
Sbjct: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGF 300

Query: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 340
           EKALMACCGYGGAPNNYNVKATCGQPGYSICANP ++IV
Sbjct: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIV 339

[Close]

BLAST of CmaCh14G009500 vs. ExPASy TrEMBL
Match: A0A6J1DWR3 (GDSL esterase/lipase At3g62280 OS=Momordica charantia OX=3673 GN=LOC111023851 PE=3 SV=1)

HSP 1 Score: 595.1 bits (1533), Expect = 1.8e-166
Identity = 291/342 (85.09%), Postives = 307/342 (89.77%), Query Frame = 0


Query: 1   MTKSRSRTTTLMFDPKH-NPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAG 60
           MT SRSR ++  FDPK  NPF VCVISLAIFIPS+    +PPTLINFGDSNSDTGGVLAG
Sbjct: 7   MTISRSRNSS--FDPKKLNPFLVCVISLAIFIPSSCSDLSPPTLINFGDSNSDTGGVLAG 66

Query: 61  TGLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVS 120
           TGLPI LPHGITFFH GTGR GDGRLIIDFFCE+LKL YLSPYLESLAPNFTSGVNFAVS
Sbjct: 67  TGLPISLPHGITFFHRGTGRLGDGRLIIDFFCEELKLTYLSPYLESLAPNFTSGVNFAVS 126

Query: 121 GATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLG-KTKN-IMDEEGFKNGVYMIDIGQND 180
           GATTLPQFVPFALD+Q+RQFIHFKNRSL LQSLG K +N +MDEEGF+NG YMIDIGQND
Sbjct: 127 GATTLPQFVPFALDIQVRQFIHFKNRSLELQSLGYKRENYMMDEEGFRNGAYMIDIGQND 186

Query: 181 LLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHP 240
           LLVALYASNLTYKPV QKIPSF+AEIKLAIQNLY NGGRKFWIHNTGP+GCSPKELALHP
Sbjct: 187 LLVALYASNLTYKPVVQKIPSFVAEIKLAIQNLYGNGGRKFWIHNTGPIGCSPKELALHP 246

Query: 241 HTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDV 300
           H  +DVDQIGCLRVHNQVAKSFNK LKNVCKQLRSQ KDA +VYVDVYTIKYNLF HP  
Sbjct: 247 HNKRDVDQIGCLRVHNQVAKSFNKALKNVCKQLRSQLKDATIVYVDVYTIKYNLFAHPKT 306

Query: 301 YGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 340
           YGFE  LM CCGYGG PNNYNVKATCGQPGYS+CANP +SIV
Sbjct: 307 YGFENPLMGCCGYGGPPNNYNVKATCGQPGYSLCANPSKSIV 346

[Close]

BLAST of CmaCh14G009500 vs. ExPASy TrEMBL
Match: A0A1S3BGX7 (GDSL esterase/lipase At3g62280 OS=Cucumis melo OX=3656 GN=LOC103489899 PE=3 SV=1)

HSP 1 Score: 521.2 bits (1341), Expect = 3.4e-144
Identity = 258/298 (86.58%), Postives = 269/298 (90.27%), Query Frame = 0


Query: 1   MTKSRSRTTTL-MFDP--KHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVL 60
           MTKSRSRTTT   FDP  K N FW  VISLAIFIPSTS   T PTLINFGDSNSDTGGVL
Sbjct: 32  MTKSRSRTTTTSSFDPKKKKNHFWFYVISLAIFIPSTS--STSPTLINFGDSNSDTGGVL 91

Query: 61  AGTGLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFA 120
           AG GLPIGLPHGITFFH GTGR GDGRLIIDFFCE+LKL YLSPYLE+LAPNFTSGVNFA
Sbjct: 92  AGAGLPIGLPHGITFFHRGTGRLGDGRLIIDFFCEELKLSYLSPYLEALAPNFTSGVNFA 151

Query: 121 VSGATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQND 180
           VSGATTLPQFVPFALDVQ+RQFIHFKNRSL LQS GK + +MDEEGFK G+YMIDIGQND
Sbjct: 152 VSGATTLPQFVPFALDVQVRQFIHFKNRSLELQSFGKIEKMMDEEGFKKGIYMIDIGQND 211

Query: 181 LLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHP 240
           +LVALY SNLTYK VAQKIPSFLA+IKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHP
Sbjct: 212 VLVALYQSNLTYKSVAQKIPSFLAQIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHP 271

Query: 241 HTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHP 296
           HTHKDVD+IGCLRVHNQVAK FNK LKNVCK+LRSQ  DAIVVYVD+YTIKYNLF HP
Sbjct: 272 HTHKDVDKIGCLRVHNQVAKFFNKGLKNVCKELRSQLNDAIVVYVDIYTIKYNLFAHP 327

[Close]

BLAST of CmaCh14G009500 vs. ExPASy TrEMBL
Match: A0A2I4G2K2 (GDSL esterase/lipase At3g62280 OS=Juglans regia OX=51240 GN=LOC109004139 PE=3 SV=2)

HSP 1 Score: 499.6 bits (1285), Expect = 1.0e-137
Identity = 236/319 (73.98%), Postives = 269/319 (84.33%), Query Frame = 0


Query: 21  WVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGRF 80
           WVCV  L +F  S   +   P LINFGDSNSDTGGVLAGTGLPIGLPHGITFFH GTGR 
Sbjct: 15  WVCVF-LGMFANSKCSSNIRPILINFGDSNSDTGGVLAGTGLPIGLPHGITFFHSGTGRL 74

Query: 81  GDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQFI 140
           GDGRL+IDF CE L L YLSPYL+SL+PNFTSGVNFAVSGAT LPQFVPFALDVQ+RQFI
Sbjct: 75  GDGRLVIDFVCEHLNLSYLSPYLDSLSPNFTSGVNFAVSGATLLPQFVPFALDVQVRQFI 134

Query: 141 HFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFL 200
            F+ RSL L SLG ++N++DE+G +N +YMIDIG+NDLL+ALYA+NLTY PV +K+PSFL
Sbjct: 135 RFRKRSLELLSLG-SRNLIDEDGIRNALYMIDIGENDLLLALYATNLTYAPVIEKVPSFL 194

Query: 201 AEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFN 260
           AEIKLAIQN+Y  GGR+FWIHNTGPLGC+PKELALHPH + D+D+IGCLR+HN VAK+FN
Sbjct: 195 AEIKLAIQNIYQYGGRRFWIHNTGPLGCAPKELALHPHNNTDLDRIGCLRIHNDVAKAFN 254

Query: 261 KDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGYGGAPNNYNVK 320
           + + NVCK+LRS FKDA +VYVD+YTIKY LF     YGFE   +ACCGYGG PNNYNVK
Sbjct: 255 EGVNNVCKELRSVFKDATIVYVDIYTIKYKLFAKYKKYGFEDPFVACCGYGGPPNNYNVK 314

Query: 321 ATCGQPGYSICANPLRSIV 340
           ATCGQPGYSIC N  RSIV
Sbjct: 315 ATCGQPGYSICRNVSRSIV 331

[Close]

BLAST of CmaCh14G009500 vs. NCBI nr
Match: XP_022983405.1 (GDSL esterase/lipase At3g62280-like [Cucurbita maxima])

HSP 1 Score: 708.4 bits (1827), Expect = 3.1e-200
Identity = 339/339 (100.00%), Postives = 339/339 (100.00%), Query Frame = 0


Query: 1   MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60
           MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT
Sbjct: 12  MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 71

Query: 61  GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120
           GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG
Sbjct: 72  GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 131

Query: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180
           ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV
Sbjct: 132 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 191

Query: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240
           ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH
Sbjct: 192 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 251

Query: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 300
           KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF
Sbjct: 252 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 311

Query: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 340
           EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV
Sbjct: 312 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 350

[Close]

BLAST of CmaCh14G009500 vs. NCBI nr
Match: XP_022935258.1 (GDSL esterase/lipase At3g62280-like [Cucurbita moschata])

HSP 1 Score: 696.0 bits (1795), Expect = 1.6e-196
Identity = 332/339 (97.94%), Postives = 335/339 (98.82%), Query Frame = 0


Query: 1   MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60
           MTKSRSRTTTLMFDP+HNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT
Sbjct: 1   MTKSRSRTTTLMFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60

Query: 61  GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120
           GLPIGLPHGITFFH GTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG
Sbjct: 61  GLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120

Query: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180
           ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV
Sbjct: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180

Query: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240
           ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH
Sbjct: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240

Query: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 300
           KDVDQIGCLRVHNQVAKSFNKDLKNVCK LRSQFKDAIVVYVDVYTIKYNLF HPDVYGF
Sbjct: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGF 300

Query: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 340
           EKALMACCGYGGAPNNYNVKATCGQPGYSICANP ++IV
Sbjct: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIV 339

[Close]

BLAST of CmaCh14G009500 vs. NCBI nr
Match: KAG7018037.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 691.8 bits (1784), Expect = 3.0e-195
Identity = 329/339 (97.05%), Postives = 334/339 (98.53%), Query Frame = 0


Query: 1   MTKSRSRTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60
           MTKSRSRTTTLMFDP+HNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT
Sbjct: 1   MTKSRSRTTTLMFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGT 60

Query: 61  GLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120
           GLPIGLPHGITFFH GTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG
Sbjct: 61  GLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSG 120

Query: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180
           ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV
Sbjct: 121 ATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLV 180

Query: 181 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240
           ALYASNL+YKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH
Sbjct: 181 ALYASNLSYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 240

Query: 241 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 300
           KDVDQIGCL+VHNQVAKSFNKDLKNVCK LRSQFKDAIVVYVDVYTIKYNLF HPD YGF
Sbjct: 241 KDVDQIGCLKVHNQVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDAYGF 300

Query: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 340
           EKALMACCGYGGAPNNYNVKATCGQPGYSICANP ++IV
Sbjct: 301 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIV 339

[Close]

BLAST of CmaCh14G009500 vs. NCBI nr
Match: XP_023529129.1 (GDSL esterase/lipase At3g62280-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 682.2 bits (1759), Expect = 2.4e-192
Identity = 329/342 (96.20%), Postives = 334/342 (97.66%), Query Frame = 0


Query: 1   MTKSRSR-TTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAG 60
           MTKSRSR TTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAG
Sbjct: 1   MTKSRSRTTTTLMFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAG 60

Query: 61  TGLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVS 120
           TGLPIGLPHGITFFH GTGRFGDGRLIIDFFCEDLKL YLSPYLESLAPNFTSGVNFAVS
Sbjct: 61  TGLPIGLPHGITFFHRGTGRFGDGRLIIDFFCEDLKLSYLSPYLESLAPNFTSGVNFAVS 120

Query: 121 GATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLG--KTKNIMDEEGFKNGVYMIDIGQND 180
           GATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLG  KTKNIMDEEGFK GVYMIDIGQND
Sbjct: 121 GATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGICKTKNIMDEEGFKKGVYMIDIGQND 180

Query: 181 LLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHP 240
           LLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHP
Sbjct: 181 LLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHP 240

Query: 241 HTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDV 300
           HTHKD+DQIGCL+VHNQVAKSFNKDLKN+CKQLRSQFKDAIVVYVDVYTIKYNLF HPDV
Sbjct: 241 HTHKDIDQIGCLKVHNQVAKSFNKDLKNMCKQLRSQFKDAIVVYVDVYTIKYNLFAHPDV 300

Query: 301 YGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSIV 340
           YGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANP ++IV
Sbjct: 301 YGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPSKAIV 342

[Close]

BLAST of CmaCh14G009500 vs. NCBI nr
Match: KAG6581315.1 (GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 671.8 bits (1732), Expect = 3.2e-189
Identity = 318/328 (96.95%), Postives = 323/328 (98.48%), Query Frame = 0


Query: 12  MFDPKHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 71
           MFDP+HNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT
Sbjct: 1   MFDPRHNPFWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGIT 60

Query: 72  FFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 131
           FFH GTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA
Sbjct: 61  FFHRGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFA 120

Query: 132 LDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKP 191
           LDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNL+YKP
Sbjct: 121 LDVQIRQFIHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLSYKP 180

Query: 192 VAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRV 251
           VAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL+V
Sbjct: 181 VAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLKV 240

Query: 252 HNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGYG 311
           HN VAKSFNKDLKNVCK LRSQFKDAIVVYVDVYTIKYNLF HPDVYGFEKALMACCGYG
Sbjct: 241 HNHVAKSFNKDLKNVCKDLRSQFKDAIVVYVDVYTIKYNLFAHPDVYGFEKALMACCGYG 300

Query: 312 GAPNNYNVKATCGQPGYSICANPLRSIV 340
           GAPNNYNVKATCGQPGYSICANP ++IV
Sbjct: 301 GAPNNYNVKATCGQPGYSICANPSKAIV 328

[Close]

BLAST of CmaCh14G009500 vs. TAIR 10
Match: AT3G62280.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 440.7 bits (1132), Expect = 1.1e-123
Identity = 213/320 (66.56%), Postives = 253/320 (79.06%), Query Frame = 0


Query: 24  VISLAIFIPSTSCTP----TPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGR 83
           V+ L++ + S S T       P LINFGDSNSDTGGVLAG GLPIGLPHGITFFH GTGR
Sbjct: 14  VLCLSLLVCSNSETSYKSNKKPILINFGDSNSDTGGVLAGVGLPIGLPHGITFFHRGTGR 73

Query: 84  FGDGRLIIDFFCEDLKLRYLSPYLESLAPNFTSGVNFAVSGATTLPQFVPFALDVQIRQF 143
            GDGRLI+DF+CE LK+ YLSPYL+SL+PNF  GVNFAVSGAT LP F  F L +QIRQF
Sbjct: 74  LGDGRLIVDFYCEHLKMTYLSPYLDSLSPNFKRGVNFAVSGATALPIF-SFPLAIQIRQF 133

Query: 144 IHFKNRSLHLQSLGKTKNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSF 203
           +HFKNRS  L S G+ ++++D+ GF+N +YMIDIGQNDLL+ALY SNLTY PV +KIPS 
Sbjct: 134 VHFKNRSQELISSGR-RDLIDDNGFRNALYMIDIGQNDLLLALYDSNLTYAPVVEKIPSM 193

Query: 204 LAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSF 263
           L EIK AIQ +Y  GGRKFW+HNTGPLGC+PKELA+H H   D+D IGC RVHN+VAK+F
Sbjct: 194 LLEIKKAIQTVYLYGGRKFWVHNTGPLGCAPKELAIHLHNDSDLDPIGCFRVHNEVAKAF 253

Query: 264 NKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGYGGAPNNYNV 323
           NK L ++C +LRSQFKDA +VYVD+Y+IKY L     +YGF   LMACCGYGG PNNY+ 
Sbjct: 254 NKGLLSLCNELRSQFKDATLVYVDIYSIKYKLSADFKLYGFVDPLMACCGYGGRPNNYDR 313

Query: 324 KATCGQPGYSICANPLRSIV 340
           KATCGQPG +IC +  ++IV
Sbjct: 314 KATCGQPGSTICRDVTKAIV 331

[Close]

BLAST of CmaCh14G009500 vs. TAIR 10
Match: AT1G56670.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 331.3 bits (848), Expect = 9.5e-91
Identity = 163/338 (48.22%), Postives = 224/338 (66.27%), Query Frame = 0


Query: 9   TTLMFDPKHNPFWVCVISLAIFI---PS--TSCTPTPPTLINFGDSNSDTGGVLAGTGLP 68
           T  ++    + F++ ++SLA+ I   PS   SCT   P + NFGDSNSDTGG++AG G P
Sbjct: 3   TLFLYSNTFSFFFITLVSLALLILRQPSRAASCT-ARPVIFNFGDSNSDTGGLVAGLGYP 62

Query: 69  IGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGAT 128
           IG P+G  FF   TGR  DGRL+IDF C+ L    L PYL+SL    F +G NFA++G+ 
Sbjct: 63  IGFPNGRLFFRRSTGRLSDGRLLIDFLCQSLNTSLLRPYLDSLGRTRFQNGANFAIAGSP 122

Query: 129 TLPQFVPFALDVQIRQFIHFKNRSLHLQSLGKTKN--IMDEEGFKNGVYMIDIGQNDLLV 188
           TLP+ VPF+L++Q++QF HFK+RSL L S   +     +   GFKN +YMIDIGQND + 
Sbjct: 123 TLPKNVPFSLNIQVKQFSHFKSRSLELASSSNSLKGMFISNNGFKNALYMIDIGQND-IA 182

Query: 189 ALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTH 248
             +A   +Y    + IP  + EIK +I+ LY  GGR+FWIHNTGPLGC P++L++     
Sbjct: 183 RSFARGNSYSQTVKLIPQIITEIKSSIKRLYDEGGRRFWIHNTGPLGCLPQKLSM--VKS 242

Query: 249 KDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGF 308
           KD+DQ GCL  +N  A  FN+ L ++C++LR++ +DA ++Y+D+Y IKY+L  + + YGF
Sbjct: 243 KDLDQHGCLVSYNSAATLFNQGLDHMCEELRTELRDATIIYIDIYAIKYSLIANSNQYGF 302

Query: 309 EKALMACCGYGGAPNNYNVKATCGQPGYSICANPLRSI 339
           +  LMACCGYGG P NYNVK TCG  G ++C    R I
Sbjct: 303 KSPLMACCGYGGTPYNYNVKITCGHKGSNVCEEGSRFI 336

[Close]

BLAST of CmaCh14G009500 vs. TAIR 10
Match: AT1G09390.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 330.9 bits (847), Expect = 1.2e-90
Identity = 158/302 (52.32%), Postives = 215/302 (71.19%), Query Frame = 0


Query: 40  PPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGRFGDGRLIIDFFCEDLKLRYL 99
           PP + NFGDSNSDTGG++AG G  IGLP+G +FF   TGR  DGRL+IDF C+ L    L
Sbjct: 35  PPVIFNFGDSNSDTGGLVAGLGYSIGLPNGRSFFQRSTGRLSDGRLVIDFLCQSLNTSLL 94

Query: 100 SPYLESL-APNFTSGVNFAVSGATTLPQFVPFALDVQIRQFIHFKNRSLHLQSLGK--TK 159
           +PYL+SL    F +G NFA+ G++TLP++VPFAL++Q+ QF+HFK+R+L L S+     +
Sbjct: 95  NPYLDSLVGSKFQNGANFAIVGSSTLPRYVPFALNIQLMQFLHFKSRALELASISDPLKE 154

Query: 160 NIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYKPVAQKIPSFLAEIKLAIQNLYANGGR 219
            ++ E GF+N +YMIDIGQND+  + ++  L+Y  V + IP+ ++EIK AI+ LY  GGR
Sbjct: 155 MMIGESGFRNALYMIDIGQNDIADS-FSKGLSYSRVVKLIPNVISEIKSAIKILYDEGGR 214

Query: 220 KFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLRVHNQVAKSFNKDLKNVCKQLRSQFKD 279
           KFW+HNTGPLGC P++L++  H+ K  D+ GCL  +N  AK FN+ L ++C+ LR++ K+
Sbjct: 215 KFWVHNTGPLGCLPQKLSM-VHS-KGFDKHGCLATYNAAAKLFNEGLDHMCRDLRTELKE 274

Query: 280 AIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGYGGAPNNYNVKATCGQPGYSICANPLR 339
           A +VYVD+Y IKY+L  + + YGFEK LMACCGYGG P NYNV  TCG  G   C    R
Sbjct: 275 ANIVYVDIYAIKYDLIANSNNYGFEKPLMACCGYGGPPYNYNVNITCGNGGSKSCDEGSR 333

[Close]

BLAST of CmaCh14G009500 vs. TAIR 10
Match: AT1G54790.3 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 6.4e-71
Identity = 141/315 (44.76%), Postives = 196/315 (62.22%), Query Frame = 0


Query: 22  VCVISLAIFIPST-SCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGRF 81
           V  I +++F+PS+ S     P +INFGDSNSDTG +++     +  P+G T+F+  +GR+
Sbjct: 11  VLFILISLFLPSSFSIILKYPAIINFGDSNSDTGNLISAGIENVNPPYGQTYFNLPSGRY 70

Query: 82  GDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPFALDVQ 141
            DGRLI+DF  +++ L +L+PYL+SL  PNF  G NFA +G+T LP       PF+ D+Q
Sbjct: 71  CDGRLIVDFLLDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDLQ 130

Query: 142 IRQFIHFKNRSLHLQSLGKT-----KNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTYK 201
           I QFI FK+R++ L  L KT     K +   + +  G+YMIDIGQND+  A Y+   T  
Sbjct: 131 ISQFIRFKSRAIEL--LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK--TLD 190

Query: 202 PVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCLR 261
            V   IPS L   +  ++ LY  GGR  WIHNTGPLGC  + +A        +D+ GC+ 
Sbjct: 191 QVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCVS 250

Query: 262 VHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGFEKALMACCGY 321
            HNQ AK FN  L  +  + ++Q+ DA V YVD+++IK NL  +   +GFEK LMACCG 
Sbjct: 251 SHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCGV 310

Query: 322 GGAPNNYNVKATCGQ 326
           GGAP NY+ + TCGQ
Sbjct: 311 GGAPLNYDSRITCGQ 321

[Close]

BLAST of CmaCh14G009500 vs. TAIR 10
Match: AT1G54790.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 265.0 bits (676), Expect = 8.4e-71
Identity = 141/316 (44.62%), Postives = 192/316 (60.76%), Query Frame = 0


Query: 20  FWVCVISLAIFIPSTSCTPTPPTLINFGDSNSDTGGVLAGTGLPIGLPHGITFFHHGTGR 79
           F+V +  ++    S S     P+  NFGDSNSDTG ++AG G+ + LP+G   F   + R
Sbjct: 9   FYVILFFISSLQISNSIDFNYPSAFNFGDSNSDTGDLVAGLGIRLDLPNGQNSFKTSSQR 68

Query: 80  FGDGRLIIDFFCEDLKLRYLSPYLESLA-PNFTSGVNFAVSGATTLP----QFVPFALDV 139
           F DGRL+IDF  +++ L +L+PYL+SL  PNF  G NFA +G+T LP       PF+ D+
Sbjct: 69  FCDGRLVIDFLMDEMDLPFLNPYLDSLGLPNFKKGCNFAAAGSTILPANPTSVSPFSFDL 128

Query: 140 QIRQFIHFKNRSLHLQSLGKT-----KNIMDEEGFKNGVYMIDIGQNDLLVALYASNLTY 199
           QI QFI FK+R++ L  L KT     K +   + +  G+YMIDIGQND+  A Y+   T 
Sbjct: 129 QISQFIRFKSRAIEL--LSKTGRKYEKYLPPIDYYSKGLYMIDIGQNDIAGAFYSK--TL 188

Query: 200 KPVAQKIPSFLAEIKLAIQNLYANGGRKFWIHNTGPLGCSPKELALHPHTHKDVDQIGCL 259
             V   IPS L   +  ++ LY  GGR  WIHNTGPLGC  + +A        +D+ GC+
Sbjct: 189 DQVLASIPSILETFEAGLKRLYEEGGRNIWIHNTGPLGCLAQNIAKFGTDSTKLDEFGCV 248

Query: 260 RVHNQVAKSFNKDLKNVCKQLRSQFKDAIVVYVDVYTIKYNLFVHPDVYGFEKALMACCG 319
             HNQ AK FN  L  +  + ++Q+ DA V YVD+++IK NL  +   +GFEK LMACCG
Sbjct: 249 SSHNQAAKLFNLQLHAMSNKFQAQYPDANVTYVDIFSIKSNLIANYSRFGFEKPLMACCG 308

Query: 320 YGGAPNNYNVKATCGQ 326
            GGAP NY+ + TCGQ
Sbjct: 309 VGGAPLNYDSRITCGQ 320

The following BLAST results are available for this feature:

BLAST of CmaCh14G009500 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q6NLP7	1.6e-122	66.56	GDSL esterase/lipase At3g62280 OS=Arabidopsis thaliana OX=3702 GN=At3g62280 PE=2...	[more]
Q9FXB6	1.3e-89	48.22	GDSL esterase/lipase LIP-4 OS=Arabidopsis thaliana OX=3702 GN=LIP4 PE=2 SV=1	[more]
O80522	1.7e-89	52.32	GDSL esterase/lipase At1g09390 OS=Arabidopsis thaliana OX=3702 GN=At1g09390 PE=2...	[more]
Q3ECP6	3.5e-66	42.11	GDSL esterase/lipase At1g54790 OS=Arabidopsis thaliana OX=3702 GN=At1g54790 PE=2...	[more]
Q9FXE5	3.0e-65	45.24	Alpha-L-fucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=FXG1 PE=2 SV=1	[more]

BLAST of CmaCh14G009500 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J5S7	1.5e-200	100.00	GDSL esterase/lipase At3g62280-like OS=Cucurbita maxima OX=3661 GN=LOC111482013 ...	[more]
A0A6J1FA19	7.6e-197	97.94	GDSL esterase/lipase At3g62280-like OS=Cucurbita moschata OX=3662 GN=LOC11144219...	[more]
A0A6J1DWR3	1.8e-166	85.09	GDSL esterase/lipase At3g62280 OS=Momordica charantia OX=3673 GN=LOC111023851 PE...	[more]
A0A1S3BGX7	3.4e-144	86.58	GDSL esterase/lipase At3g62280 OS=Cucumis melo OX=3656 GN=LOC103489899 PE=3 SV=1	[more]
A0A2I4G2K2	1.0e-137	73.98	GDSL esterase/lipase At3g62280 OS=Juglans regia OX=51240 GN=LOC109004139 PE=3 SV...	[more]

BLAST of CmaCh14G009500 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022983405.1	3.1e-200	100.00	GDSL esterase/lipase At3g62280-like [Cucurbita maxima]	[more]
XP_022935258.1	1.6e-196	97.94	GDSL esterase/lipase At3g62280-like [Cucurbita moschata]	[more]
KAG7018037.1	3.0e-195	97.05	GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023529129.1	2.4e-192	96.20	GDSL esterase/lipase At3g62280-like [Cucurbita pepo subsp. pepo]	[more]
KAG6581315.1	3.2e-189	96.95	GDSL esterase/lipase, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmaCh14G009500 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62280.1	1.1e-123	66.56	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G56670.1	9.5e-91	48.22	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G09390.1	1.2e-90	52.32	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G54790.3	6.4e-71	44.76	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]
AT1G54790.1	8.4e-71	44.62	GDSL-like Lipase/Acylhydrolase superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001087	GDSL lipase/esterase	PFAM	PF00657	Lipase_GDSL	coord: 44..291 e-value: 3.4E-30 score: 106.2
IPR036514	SGNH hydrolase superfamily	GENE3D	3.40.50.1110	SGNH hydrolase	coord: 35..339 e-value: 3.3E-56 score: 193.0
None	No IPR available	PANTHER	PTHR22835	ZINC FINGER FYVE DOMAIN CONTAINING PROTEIN	coord: 20..339
None	No IPR available	PANTHER	PTHR22835:SF507	BNAC06G18430D PROTEIN	coord: 20..339
None	No IPR available	SUPERFAMILY	52266	SGNH hydrolase	coord: 41..284
IPR035669	GDSL lipase/esterase-like, plant	CDD	cd01837	SGNH_plant_lipase_like	coord: 41..334 e-value: 3.66399E-97 score: 288.745

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh14G009500.1	CmaCh14G009500.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042742	defense response to bacterium
biological_process	GO:0002229	defense response to oomycetes
biological_process	GO:0006468	protein phosphorylation
biological_process	GO:0007178	transmembrane receptor protein serine/threonine kinase signaling pathway
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0005886	plasma membrane
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0030246	carbohydrate binding
molecular_function	GO:0016788	hydrolase activity, acting on ester bonds
molecular_function	GO:0004675	transmembrane receptor protein serine/threonine kinase activity