CmaCh13G003310 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh13G003310
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	subtilisin-like protease SBT5.4
Location	Cma_Chr13: 3848857 .. 3850545 (+)
RNA-Seq Expression	CmaCh13G003310
Synteny	CmaCh13G003310

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACCTCCGCCAGAGTTAGCGACAACACAATGAACCTCATGCTCGATGGCGACTCCCCTATCTTCGCTCCAGCCACCCCGTTCATATATGGGTCAGGGCACATCCTCCCCACTAGCGCCATCGACCCCGGCCTTGTCTACGACCTTTCCCCCAACAATTTACTTGGAATTCATTTGTGCGAGTGGCTACAAGGAGAAGAGCATTCGAGTATTCGCTGATGGAAATTTCAAATTCCCTATTTCTGATTCTATTTTGAACTTTAATTACCCTTCCATTGAACTTGACTGGGAGTGCCACCGTTACTAGAAGGCTGAAGAATGTTGGCAAGCTGGGGGTTTACCGAGTTAGAGTTCGGCAGCCGGAAGGAGTTAAGGTTTCAGTGAAGCCAAGCGTTTTGAAGTTTGAGAAGATTGGAGAGGAGAAGAGGTTTGAATTGATGATGGCCAGAGCTGTGGCGAAGGGTCAAATTTGTTACGGTAAGCTGGTTTGGACCGACTGCAAGCACTTTGTTAGGAGTCTAATTGTGGTTTCTTCTAGCTTTTTATGAACCCATTTTGTTAGGAGGGATTACTAGATTTCAAGTTTTGTTTTTTGTGCTTGAAATCATATTCGTTTTTTTAGATATGAGCTAGTTTCAAGGAATTCTATCTTTTTATTTGAAACATTTCCCTCTTTTTCCTTTTTAGTTTAATCAATTGTTTTAGTTTAACCCATGGTCGAAACAAACAATCTCTTTTCTTTTCATCTGTCTATTTCACTGAACCTCTCTATGGGATGGTGCCCAGATCTTTTGTCTTTCATGTGGATTGAAATTTACTGGACAAGGGATTTTGCTACCTTAGGACCATTATAATTATGGCCACCATTCACCGGGGCTGGCGGCCCGCTCCCTTGTTACCGGGTCATAAACTTCCTTGACTTTCCAGTAGTAGGCGTCAGCCTACATACATGGCCTTAAGACTTTGCGGGTGGTGATTTTTCTGCTACCACCATGTTAGCCCCTTTGAGCTTATTCGTATTTTAATCGAAGAGAGGTCAGTTCGACCAAGAGATGGATACGGGTGATAGAAAAAAAGAGGCAAGTCCCTGATTCAAATTATAGTGTGAGAGGGGTCGAGCTTGGGTCGAGTTTCTATTTCCATGTACGGGGGCAAGAATCTTTCTATAACAACCCAAAATTTTCTACTTAATTATACATATCATAAACATTGAATGGAGAAATATTCATTTAAATCTTAAAAAACATAACCTTTAGATTAATTAGAATTTTTTTTATTTAATTCTTTTCATTCATTACAAAATTGAATTAGACTTGATATATTATTTTGATATGATAGATTATTTCAATATTATCATTAATATGAGTTGCCCGGGACTCGAACTCGGAACTAGTCAGATGGAGTAGATAACTTACTCTTTAAAAAACTAAGTAAAAAAAATCTCTCCAAACCATGGTAAAGATTTATGATACATGTTTAGGGTGTACTCAATGTGTCCGAGCTTGCCCCACAGATGTATTAGAAATGATACCTTGGGGCGGATGAAAAGCTAAGCAAATAGCTTCGGCTCAATGAACAGAAGACTGTGTTGGTTGTAAGAGATGTGAATCTGCCTGTCCAACGGATTTCTTGAGTTTTCGCGTTTATTTATGGCATGAAACAACTCGAAGCATGGGTCTAGCGTATTGA

mRNA sequence

ATGACCTCCGCCAGAGTTAGCGACAACACAATGAACCTCATGCTCGATGGCGACTCCCCTATCTTCGCTCCAGCCACCCCGTTCATATATGGGTCAGGGCACATCCTCCCCACTAGCGCCATCGACCCCGGCCTTGTCTACGACCTTTCCCCCAACAATTTACTTGGAATTCATTTGTGCGATGCCACCGTTACTAGAAGGCTGAAGAATGTTGGCAAGCTGGGGGTTTACCGAGTTAGAGTTCGGCAGCCGGAAGGAGTTAAGGTTTCAGTGAAGCCAAGCGTTTTGAAGTTTGAGAAGATTGGAGAGGAGAAGAGGTTTGAATTGATGATGGCCAGAGCTGTGGCGAAGGGTCAAATTTGTTACGGTAAGCTGGTTTGGACCGACTGCAAGCACTTTGTTAGGAGTCTAATTGTGAGATGTGAATCTGCCTGTCCAACGGATTTCTTGAGTTTTCGCGTTTATTTATGGCATGAAACAACTCGAAGCATGGGTCTAGCGTATTGA

Coding sequence (CDS)

ATGACCTCCGCCAGAGTTAGCGACAACACAATGAACCTCATGCTCGATGGCGACTCCCCTATCTTCGCTCCAGCCACCCCGTTCATATATGGGTCAGGGCACATCCTCCCCACTAGCGCCATCGACCCCGGCCTTGTCTACGACCTTTCCCCCAACAATTTACTTGGAATTCATTTGTGCGATGCCACCGTTACTAGAAGGCTGAAGAATGTTGGCAAGCTGGGGGTTTACCGAGTTAGAGTTCGGCAGCCGGAAGGAGTTAAGGTTTCAGTGAAGCCAAGCGTTTTGAAGTTTGAGAAGATTGGAGAGGAGAAGAGGTTTGAATTGATGATGGCCAGAGCTGTGGCGAAGGGTCAAATTTGTTACGGTAAGCTGGTTTGGACCGACTGCAAGCACTTTGTTAGGAGTCTAATTGTGAGATGTGAATCTGCCTGTCCAACGGATTTCTTGAGTTTTCGCGTTTATTTATGGCATGAAACAACTCGAAGCATGGGTCTAGCGTATTGA

Protein sequence

MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLCDATVTRRLKNVGKLGVYRVRVRQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIVRCESACPTDFLSFRVYLWHETTRSMGLAY

Homology

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BLAST of CmaCh13G003310 vs. ExPASy Swiss-Prot
Match: F4JXC5 (Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 SV=1)

HSP 1 Score: 98.2 bits (243), Expect = 9.3e-20
Identity = 66/180 (36.67%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MT++R  +N    M+D     F  A PF YGSGH+ P  A  PGLVYDL+  + L     
Sbjct: 597 MTTSRTRNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCA 656

Query: 61  ---------------------GIHLCD--------------ATVTRRLKNVGKLGVYRVR 120
                                G +L D               TVTR+LKNVG    Y  R
Sbjct: 657 VGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNAR 716

Query: 121 VRQPEGVKVSVKPSVLKFEKIGEEKRFEL-MMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
            R+P GV+VSV+P  L F K GE K F++ +    V      +G+L WTD  H+VRS IV
Sbjct: 717 FREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIV 773

[Close]

BLAST of CmaCh13G003310 vs. ExPASy Swiss-Prot
Match: Q9ZSP5 (Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 2.7e-19
Identity = 63/184 (34.24%), Postives = 95/184 (51.63%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLC 60
           MT+A + D+    + +  +     ATPF +G+GH+ P  A++PGLVYDL   + L   LC
Sbjct: 591 MTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNF-LC 650

Query: 61  D------------------------------------------ATVTRRLKNVGKLGVYR 120
                                                       TV+R +KNVG+  +Y 
Sbjct: 651 SLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYT 710

Query: 121 VRVRQPEGVKVSVKPSVLKFEKIGEEKRFELMMARA---VAKGQICYGKLVWTDCKHFVR 140
           V+V  P+GV V+VKP+ L F K+GE+K F++++ ++   VAKG + +G+LVW+D KH VR
Sbjct: 711 VKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV-FGELVWSDKKHRVR 769

[Close]

BLAST of CmaCh13G003310 vs. ExPASy Swiss-Prot
Match: I1N462 (Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 PE=1 SV=3)

HSP 1 Score: 84.0 bits (206), Expect = 1.8e-15
Identity = 60/181 (33.15%), Postives = 78/181 (43.09%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MT+A   DNT   + D      A A  F YGSGH+ P  AI+PGLVYDLS  + L     
Sbjct: 606 MTTATTLDNTNRPIQDAFDKTLADA--FAYGSGHVRPDLAIEPGLVYDLSLTDYLNFLCA 665

Query: 61  ------------------------------------GIHLCDATVTRRLKNVGKLGVYRV 120
                                                + L   T+ R + NVG    Y V
Sbjct: 666 SGYDQQLISALNFNRTFICSGSHSVNDLNYPSITLPNLRLKPVTIARTVTNVGPPSTYTV 725

Query: 121 RVRQPEGVKVSVKPSVLKFEKIGEEKRFELMM--ARAVAKGQICYGKLVWTDCKHFVRSL 139
             R P G  ++V P  L F KIGE K F++++  + A  + +  +G L WTD KH VRS 
Sbjct: 726 STRSPNGYSIAVVPPSLTFTKIGERKTFKVIVQASSAATRRKYEFGDLRWTDGKHIVRSP 784

[Close]

BLAST of CmaCh13G003310 vs. ExPASy Swiss-Prot
Match: A9JQS7 (Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 5.9e-14
Identity = 60/180 (33.33%), Postives = 79/180 (43.89%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLC 60
           MTSA V DNT +L+  G  P    ATPF YGSGH+ P ++++PGLVYD S  ++L     
Sbjct: 575 MTSATVMDNTHSLI--GRDPNGTQATPFDYGSGHVNPVASLNPGLVYDFSSQDVLNFLCS 634

Query: 61  DATVTRRLKNV----------------------------GKLGVYR-------------V 120
           +     +LKN+                            G L VYR              
Sbjct: 635 NGASPAQLKNLTGELTQCQKSPTASYNFNYPSIGVSNLNGSLSVYRTVTYYGQEPTEYFA 694

Query: 121 RVRQPEGVKVSVKPSVLKFEKIGEEKRFEL-MMARAVAKGQICYGKLVWTDCKHFVRSLI 139
            V +P GV V V P+ LKF K GE+  F +       + G   +G L W + K  VRS I
Sbjct: 695 SVERPSGVIVRVTPAKLKFWKAGEKITFRIDFTPFKNSNGNFVFGALTWNNGKQRVRSPI 752

[Close]

BLAST of CmaCh13G003310 vs. ExPASy Swiss-Prot
Match: Q9FK76 (Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.9e-12
Identity = 52/160 (32.50%), Postives = 68/160 (42.50%), Query Frame = 0


Query: 24  PATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLCD---------------------- 83
           PA PF  GSGH  PT A DPGLVYD S    L ++ C                       
Sbjct: 628 PANPFALGSGHFRPTKAADPGLVYDASYRAYL-LYGCSVNITNIDPTFKCPSKIPPGYNH 687

Query: 84  -------------ATVTRRLKNVG---KLGVYRVRVRQPEGVKVSVKPSVLKFEKIGEEK 139
                         TV R + NVG       Y   V+ P G+ V   P++L F +IG+++
Sbjct: 688 NYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQ 747

[Close]

BLAST of CmaCh13G003310 vs. ExPASy TrEMBL
Match: A0A6J1EG66 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434032 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 4.9e-48
Identity = 113/178 (63.48%), Postives = 120/178 (67.42%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MTSARVSDNTMNLMLDG SPIFAPATPF+YGSGHI PT AIDPGLVYDLSPN+ L     
Sbjct: 593 MTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 652

Query: 61  -------------GIHLC---------------------DATVTRRLKNVGKLGVYRVRV 120
                        G   C                       TV+RRLKNVG  GVYRVRV
Sbjct: 653 SGYTEKNLRVFTDGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRV 712

Query: 121 RQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           +QPEGVKVSVKP+VLKFEKIGEEK FEL M  AVA+GQI YG L+WTD KHFVRS IV
Sbjct: 713 QQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIV 770

[Close]

BLAST of CmaCh13G003310 vs. ExPASy TrEMBL
Match: A0A6J1EK22 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434033 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 4.9e-48
Identity = 113/178 (63.48%), Postives = 120/178 (67.42%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MTSARVSDNTMNLMLDG SPIFAPATPF+YGSGHI PT AIDPGLVYDLSPN+ L     
Sbjct: 451 MTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 510

Query: 61  -------------GIHLC---------------------DATVTRRLKNVGKLGVYRVRV 120
                        G   C                       TV+RRLKNVG  GVYRVRV
Sbjct: 511 SGYTEKNLRVFTDGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRV 570

Query: 121 RQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           +QPEGVKVSVKP+VLKFEKIGEEK FEL M  AVA+GQI YG L+WTD KHFVRS IV
Sbjct: 571 QQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIV 628

[Close]

BLAST of CmaCh13G003310 vs. ExPASy TrEMBL
Match: A0A6J1F4N6 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111440718 PE=3 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 4.9e-48
Identity = 113/178 (63.48%), Postives = 120/178 (67.42%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MTSARVSDNTMNLMLDG SPIFAPATPF+YGSGHI PT AIDPGLVYDLSPN+ L     
Sbjct: 69  MTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 128

Query: 61  -------------GIHLC---------------------DATVTRRLKNVGKLGVYRVRV 120
                        G   C                       TV+RRLKNVG  GVYRVRV
Sbjct: 129 SGYTEKNLRVFTDGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRV 188

Query: 121 RQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           +QPEGVKVSVKP+VLKFEKIGEEK FEL M  AVA+GQI YG L+WTD KHFVRS IV
Sbjct: 189 QQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIV 246

[Close]

BLAST of CmaCh13G003310 vs. ExPASy TrEMBL
Match: A0A6J1IAG7 (subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111471620 PE=3 SV=1)

HSP 1 Score: 194.1 bits (492), Expect = 4.6e-46
Identity = 110/179 (61.45%), Postives = 118/179 (65.92%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLC 60
           MTSA +SDNTMNLMLDG SPIFAPATPFIYGSGHI PT AIDPGLVYDLSPN+ L   LC
Sbjct: 410 MTSATISDNTMNLMLDGGSPIFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEF-LC 469

Query: 61  ----------------------------------------DATVTRRLKNVGKLGVYRVR 120
                                                     T+TRRLKNVG+ GVYRV 
Sbjct: 470 ASGYKEKNIRVFADENFKCPVFCSILNFNYPSIGVQNLTGSVTLTRRLKNVGRPGVYRVI 529

Query: 121 VRQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           VR+PEGVKVSVKP VLKF KIGEEKRFEL M  AVA+GQI YG L+WTD KHFV+S IV
Sbjct: 530 VRRPEGVKVSVKPRVLKFWKIGEEKRFELTMTGAVAEGQIGYGTLIWTDDKHFVKSPIV 587

[Close]

BLAST of CmaCh13G003310 vs. ExPASy TrEMBL
Match: A0A6J1EG71 (subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434037 PE=3 SV=1)

HSP 1 Score: 193.0 bits (489), Expect = 1.0e-45
Identity = 109/179 (60.89%), Postives = 118/179 (65.92%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLC 60
           MTSA +SDNTMNL+LDG SP+FAPATPFIYGSGHI PT AIDPGLVYDLSPN+ L   LC
Sbjct: 819 MTSAIISDNTMNLILDGGSPMFAPATPFIYGSGHIHPTGAIDPGLVYDLSPNDYLEF-LC 878

Query: 61  ----------------------------------------DATVTRRLKNVGKLGVYRVR 120
                                                     T+ RRLKNVG+ GVYRVR
Sbjct: 879 ASGYKEKNIRVFADENFKCPISCSIFNFNYPSIGVQNLTGSVTLKRRLKNVGRPGVYRVR 938

Query: 121 VRQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           VRQPEGVKVSVKP VLKF KIGEEKRFEL M  A+A+GQI YG L+WTD KHFVRS IV
Sbjct: 939 VRQPEGVKVSVKPRVLKFWKIGEEKRFELTMTGAMAEGQIGYGTLIWTDGKHFVRSPIV 996

[Close]

BLAST of CmaCh13G003310 vs. NCBI nr
Match: KAG6583681.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 200.7 bits (509), Expect = 1.0e-47
Identity = 113/179 (63.13%), Postives = 120/179 (67.04%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLC 60
           MTSARVSDNTMNLMLDG SPIFAPATPF+YGSGHI PT AIDPGLVYDLSPN+ L   LC
Sbjct: 584 MTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEF-LC 643

Query: 61  ----------------------------------------DATVTRRLKNVGKLGVYRVR 120
                                                     TV+RRLKNVG  GVYRVR
Sbjct: 644 ASGYTEKNLRVFTDANFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVR 703

Query: 121 VRQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           V+QPEGVKVSVKP+VLKFEKIGEEK FEL M  AVA+GQI YG L+WTD KHFVRS IV
Sbjct: 704 VQQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIV 761

[Close]

BLAST of CmaCh13G003310 vs. NCBI nr
Match: XP_022927097.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 200.7 bits (509), Expect = 1.0e-47
Identity = 113/178 (63.48%), Postives = 120/178 (67.42%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MTSARVSDNTMNLMLDG SPIFAPATPF+YGSGHI PT AIDPGLVYDLSPN+ L     
Sbjct: 593 MTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 652

Query: 61  -------------GIHLC---------------------DATVTRRLKNVGKLGVYRVRV 120
                        G   C                       TV+RRLKNVG  GVYRVRV
Sbjct: 653 SGYTEKNLRVFTDGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRV 712

Query: 121 RQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           +QPEGVKVSVKP+VLKFEKIGEEK FEL M  AVA+GQI YG L+WTD KHFVRS IV
Sbjct: 713 QQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIV 770

[Close]

BLAST of CmaCh13G003310 vs. NCBI nr
Match: XP_022933360.1 (subtilisin-like protease SBT5.4, partial [Cucurbita moschata])

HSP 1 Score: 200.7 bits (509), Expect = 1.0e-47
Identity = 113/178 (63.48%), Postives = 120/178 (67.42%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MTSARVSDNTMNLMLDG SPIFAPATPF+YGSGHI PT AIDPGLVYDLSPN+ L     
Sbjct: 69  MTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 128

Query: 61  -------------GIHLC---------------------DATVTRRLKNVGKLGVYRVRV 120
                        G   C                       TV+RRLKNVG  GVYRVRV
Sbjct: 129 SGYTEKNLRVFTDGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRV 188

Query: 121 RQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           +QPEGVKVSVKP+VLKFEKIGEEK FEL M  AVA+GQI YG L+WTD KHFVRS IV
Sbjct: 189 QQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIV 246

[Close]

BLAST of CmaCh13G003310 vs. NCBI nr
Match: XP_022927098.1 (subtilisin-like protease SBT5.4 [Cucurbita moschata])

HSP 1 Score: 200.7 bits (509), Expect = 1.0e-47
Identity = 113/178 (63.48%), Postives = 120/178 (67.42%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MTSARVSDNTMNLMLDG SPIFAPATPF+YGSGHI PT AIDPGLVYDLSPN+ L     
Sbjct: 451 MTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEFLCA 510

Query: 61  -------------GIHLC---------------------DATVTRRLKNVGKLGVYRVRV 120
                        G   C                       TV+RRLKNVG  GVYRVRV
Sbjct: 511 SGYTEKNLRVFTDGNFKCPVSGSILNFNYPSIGVQNLTGSVTVSRRLKNVGMPGVYRVRV 570

Query: 121 RQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           +QPEGVKVSVKP+VLKFEKIGEEK FEL M  AVA+GQI YG L+WTD KHFVRS IV
Sbjct: 571 QQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIV 628

[Close]

BLAST of CmaCh13G003310 vs. NCBI nr
Match: KAG6583683.1 (Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 200.3 bits (508), Expect = 1.3e-47
Identity = 113/180 (62.78%), Postives = 120/180 (66.67%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLC 60
           MTSARVSDNTMNLMLDG SPIFAPATPF+YGSGHI PT AIDPGLVYDLSPN+ L   LC
Sbjct: 317 MTSARVSDNTMNLMLDGGSPIFAPATPFVYGSGHIRPTGAIDPGLVYDLSPNDYLEF-LC 376

Query: 61  -----------------------------------------DATVTRRLKNVGKLGVYRV 120
                                                      TV+RRLKNVG  GVYRV
Sbjct: 377 ASGYTEKNLRVFTDANFKCPVSGSILNFNYPSIGVVQNLTGSVTVSRRLKNVGMPGVYRV 436

Query: 121 RVRQPEGVKVSVKPSVLKFEKIGEEKRFELMMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
           RV+QPEGVKVSVKP+VLKFEKIGEEK FEL M  AVA+GQI YG L+WTD KHFVRS IV
Sbjct: 437 RVQQPEGVKVSVKPNVLKFEKIGEEKGFELTMTGAVAEGQIGYGTLIWTDDKHFVRSPIV 495

[Close]

BLAST of CmaCh13G003310 vs. TAIR 10
Match: AT5G59810.1 (Subtilase family protein )

HSP 1 Score: 98.2 bits (243), Expect = 6.6e-21
Identity = 66/180 (36.67%), Postives = 84/180 (46.67%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLL----- 60
           MT++R  +N    M+D     F  A PF YGSGH+ P  A  PGLVYDL+  + L     
Sbjct: 597 MTTSRTRNNRRKPMVDES---FKKANPFSYGSGHVQPNKAAHPGLVYDLTTGDYLDFLCA 656

Query: 61  ---------------------GIHLCD--------------ATVTRRLKNVGKLGVYRVR 120
                                G +L D               TVTR+LKNVG    Y  R
Sbjct: 657 VGYNNTVVQLFAEDPQYTCRQGANLLDFNYPSITVPNLTGSITVTRKLKNVGPPATYNAR 716

Query: 121 VRQPEGVKVSVKPSVLKFEKIGEEKRFEL-MMARAVAKGQICYGKLVWTDCKHFVRSLIV 140
            R+P GV+VSV+P  L F K GE K F++ +    V      +G+L WTD  H+VRS IV
Sbjct: 717 FREPLGVRVSVEPKQLTFNKTGEVKIFQMTLRPLPVTPSGYVFGELTWTDSHHYVRSPIV 773

[Close]

BLAST of CmaCh13G003310 vs. TAIR 10
Match: AT2G04160.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 96.7 bits (239), Expect = 1.9e-20
Identity = 63/184 (34.24%), Postives = 95/184 (51.63%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLC 60
           MT+A + D+    + +  +     ATPF +G+GH+ P  A++PGLVYDL   + L   LC
Sbjct: 591 MTTATIMDDIPGPIQNATN---MKATPFSFGAGHVQPNLAVNPGLVYDLGIKDYLNF-LC 650

Query: 61  D------------------------------------------ATVTRRLKNVGKLGVYR 120
                                                       TV+R +KNVG+  +Y 
Sbjct: 651 SLGYNASQISVFSGNNFTCSSPKISLVNLNYPSITVPNLTSSKVTVSRTVKNVGRPSMYT 710

Query: 121 VRVRQPEGVKVSVKPSVLKFEKIGEEKRFELMMARA---VAKGQICYGKLVWTDCKHFVR 140
           V+V  P+GV V+VKP+ L F K+GE+K F++++ ++   VAKG + +G+LVW+D KH VR
Sbjct: 711 VKVNNPQGVYVAVKPTSLNFTKVGEQKTFKVILVKSKGNVAKGYV-FGELVWSDKKHRVR 769

[Close]

BLAST of CmaCh13G003310 vs. TAIR 10
Match: AT5G45650.1 (subtilase family protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-13
Identity = 52/160 (32.50%), Postives = 68/160 (42.50%), Query Frame = 0


Query: 24  PATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLCD---------------------- 83
           PA PF  GSGH  PT A DPGLVYD S    L ++ C                       
Sbjct: 628 PANPFALGSGHFRPTKAADPGLVYDASYRAYL-LYGCSVNITNIDPTFKCPSKIPPGYNH 687

Query: 84  -------------ATVTRRLKNVG---KLGVYRVRVRQPEGVKVSVKPSVLKFEKIGEEK 139
                         TV R + NVG       Y   V+ P G+ V   P++L F +IG+++
Sbjct: 688 NYPSIAVPNLKKTVTVKRTVTNVGTGNSTSTYLFSVKPPSGISVKAIPNILSFNRIGQKQ 747

[Close]

BLAST of CmaCh13G003310 vs. TAIR 10
Match: AT5G67360.1 (Subtilase family protein )

HSP 1 Score: 73.9 bits (180), Expect = 1.3e-13
Identity = 48/158 (30.38%), Postives = 75/158 (47.47%), Query Frame = 0


Query: 24  PATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGI------------------HLCDAT-- 83
           P+TPF +G+GH+ PT+A +PGL+YDL+  + LG                   + CD +  
Sbjct: 593 PSTPFDHGAGHVSPTTATNPGLIYDLTTEDYLGFLCALNYTSPQIRSVSRRNYTCDPSKS 652

Query: 84  ----------------------VTRRLKNVGKLGVYRVRV-RQPEGVKVSVKPSVLKFEK 137
                                  TR + +VG  G Y V+V  +  GVK+SV+P+VL F++
Sbjct: 653 YSVADLNYPSFAVNVDGVGAYKYTRTVTSVGGAGTYSVKVTSETTGVKISVEPAVLNFKE 712

[Close]

BLAST of CmaCh13G003310 vs. TAIR 10
Match: AT5G45640.1 (Subtilisin-like serine endopeptidase family protein )

HSP 1 Score: 71.2 bits (173), Expect = 8.7e-13
Identity = 56/184 (30.43%), Postives = 78/184 (42.39%), Query Frame = 0


Query: 1   MTSARVSDNTMNLMLDGDSPIFAPATPFIYGSGHILPTSAIDPGLVYDLSPNNLLGIHLC 60
           MT+A +++     + D D    +PA PF  GS H  PT A  PGLVYD S  + L ++ C
Sbjct: 570 MTTASMTNEDNEPIQDYDG---SPANPFALGSRHFRPTKAASPGLVYDASYQSYL-LYCC 629

Query: 61  D-----------------------------------ATVTRRLKNVGKLG----VYRVRV 120
                                                TVTR +  VG+ G    VY    
Sbjct: 630 SVGLTNLDPTFKCPSRIPPGYNLNYPSISIPYLSGTVTVTRTVTCVGRTGNSTSVYVFNA 689

Query: 121 RQPEGVKVSVKPSVLKFEKIGEEKRFELMMAR-------AVAKGQICYGKLVWTDCKHFV 139
           + P GV V  +P+VL F+KIG++KRF ++             + +  +G   WTD  H V
Sbjct: 690 QPPNGVLVKAEPNVLVFDKIGQKKRFNIIFTTQRYEFTGEARRDRYRFGWFSWTDGHHVV 749

The following BLAST results are available for this feature:

BLAST of CmaCh13G003310 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JXC5	9.3e-20	36.67	Subtilisin-like protease SBT5.4 OS=Arabidopsis thaliana OX=3702 GN=SBT5.4 PE=1 S...	[more]
Q9ZSP5	2.7e-19	34.24	Subtilisin-like protease SBT5.3 OS=Arabidopsis thaliana OX=3702 GN=AIR3 PE=2 SV=...	[more]
I1N462	1.8e-15	33.15	Subtilisin-like protease Glyma18g48580 OS=Glycine max OX=3847 GN=Glyma18g48580 P...	[more]
A9JQS7	5.9e-14	33.33	Subtilisin-like serine-protease S OS=Lotus japonicus OX=34305 GN=SbtS PE=2 SV=1	[more]
Q9FK76	1.9e-12	32.50	Subtilisin-like protease SBT5.6 OS=Arabidopsis thaliana OX=3702 GN=SBT5.6 PE=2 S...	[more]

BLAST of CmaCh13G003310 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EG66	4.9e-48	63.48	subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434032 PE...	[more]
A0A6J1EK22	4.9e-48	63.48	subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434033 PE...	[more]
A0A6J1F4N6	4.9e-48	63.48	subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111440718 PE...	[more]
A0A6J1IAG7	4.6e-46	61.45	subtilisin-like protease SBT5.4 OS=Cucurbita maxima OX=3661 GN=LOC111471620 PE=3...	[more]
A0A6J1EG71	1.0e-45	60.89	subtilisin-like protease SBT5.4 OS=Cucurbita moschata OX=3662 GN=LOC111434037 PE...	[more]

BLAST of CmaCh13G003310 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6583681.1	1.0e-47	63.13	Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022927097.1	1.0e-47	63.48	subtilisin-like protease SBT5.4 [Cucurbita moschata]	[more]
XP_022933360.1	1.0e-47	63.48	subtilisin-like protease SBT5.4, partial [Cucurbita moschata]	[more]
XP_022927098.1	1.0e-47	63.48	subtilisin-like protease SBT5.4 [Cucurbita moschata]	[more]
KAG6583683.1	1.3e-47	62.78	Subtilisin-like protease 5.4, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of CmaCh13G003310 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G59810.1	6.6e-21	36.67	Subtilase family protein	[more]
AT2G04160.1	1.9e-20	34.24	Subtilisin-like serine endopeptidase family protein	[more]
AT5G45650.1	1.3e-13	32.50	subtilase family protein	[more]
AT5G67360.1	1.3e-13	30.38	Subtilase family protein	[more]
AT5G45640.1	8.7e-13	30.43	Subtilisin-like serine endopeptidase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	2.60.40.2310		coord: 52..141 e-value: 7.8E-21 score: 76.2
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 61..139
None	No IPR available	PANTHER	PTHR43806:SF40	BNAC02G33520D PROTEIN	coord: 61..139
None	No IPR available	PANTHER	PTHR43806	PEPTIDASE S8	coord: 1..56
None	No IPR available	PANTHER	PTHR43806:SF40	BNAC02G33520D PROTEIN	coord: 1..56
IPR041469	Subtilisin-like protease, fibronectin type-III domain	PFAM	PF17766	fn3_6	coord: 62..139 e-value: 2.8E-18 score: 66.0
IPR036852	Peptidase S8/S53 domain superfamily	GENE3D	3.40.50.200	Peptidase S8/S53 domain	coord: 1..51 e-value: 1.1E-6 score: 29.7

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh13G003310.1	CmaCh13G003310.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0004252	serine-type endopeptidase activity