CmaCh11G006340 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G006340
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionTransposable element protein
LocationCma_Chr11: 3068945 .. 3069769 (+)
RNA-Seq ExpressionCmaCh11G006340
SyntenyCmaCh11G006340
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGTAGAGAATCAGACGGACAAGAAAATCAAAAGGCTGAGGTCAGACAACGGGGGAGAATATACTTATGATCCATTCCTTAAAGTATGCCGAGATGAAGGGATCGTTCGACACTTCACTGTTCCTGGTAAGCCACAACAAAATGGAGTTGCTGAGAGAATGAACCAGACATTAATAGAGAAGGTTCGATGCATATTGTCTCAAGCAGGATTGAGTAAGGCATTTTGGGCTGAGGCTCTCAGTTATGCAGTTCACTTGGTGAATCGTTTACCTGTTTCTGGAAACGGTGGGAGAACTCCGCTTGAGGTATGGTCAGGTAGTCCTGTTAGTGATTATGATAAATTACATGTGTTTGGGTGTCCTGCTTATTATCATGTGACAGACTCAAAGCTGGATCCTAGAGCAAAAAAAGCCAAGTTTATGGGCTTTAGCAAAGGTGTGAAGGGCTATAGGTTATGGTGTCCAGAAACAAGTAAGATTGTTAATAGTCGAGATGTGACATTCGATGAGTCTGGAATGTTTTTGCAGAAAATTGAAAATAATGACGAGGCATTGAAGCAGGTGGAGAAGGTGGTGTTCTCTCCTGATGTGGTTGCTCCTACTGAAGAACCTATTGACCAGGTAAATAATAACTTTGATGTCTTAGAACAGAAAGAGCAAAGTCTGGAGGAGCAAAGCCTTGTAAATGAAAGAGTGGAGGAACCTGAGTCTATCGCCAAGAATAGACCACGAAGGGTAATTCGAAAACCTGCAAGGTTTGATGATACGATAGCATATGCTTTCTCTATAATTGATGGAGTTCCCAACTTACGTATTGAGGCTTGA

mRNA sequence

ATGGTAGAGAATCAGACGGACAAGAAAATCAAAAGGCTGAGGTCAGACAACGGGGGAGAATATACTTATGATCCATTCCTTAAAGTATGCCGAGATGAAGGGATCGTTCGACACTTCACTGTTCCTGGTAAGCCACAACAAAATGGAGTTGCTGAGAGAATGAACCAGACATTAATAGAGAAGGTTCGATGCATATTGTCTCAAGCAGGATTGAGTAAGGCATTTTGGGCTGAGGCTCTCAGTTATGCAGTTCACTTGGTGAATCGTTTACCTGTTTCTGGAAACGGTGGGAGAACTCCGCTTGAGGTATGGTCAGGTAGTCCTGTTAGTGATTATGATAAATTACATGTGTTTGGGTGTCCTGCTTATTATCATGTGACAGACTCAAAGCTGGATCCTAGAGCAAAAAAAGCCAAGTTTATGGGCTTTAGCAAAGGTGTGAAGGGCTATAGGTTATGGTGTCCAGAAACAAGTAAGATTGTTAATAGTCGAGATGTGACATTCGATGAGTCTGGAATGTTTTTGCAGAAAATTGAAAATAATGACGAGGCATTGAAGCAGGTGGAGAAGGTGGTGTTCTCTCCTGATGTGGTTGCTCCTACTGAAGAACCTATTGACCAGGTAAATAATAACTTTGATGTCTTAGAACAGAAAGAGCAAAGTCTGGAGGAGCAAAGCCTTGTAAATGAAAGAGTGGAGGAACCTGAGTCTATCGCCAAGAATAGACCACGAAGGGTAATTCGAAAACCTGCAAGGTTTGATGATACGATAGCATATGCTTTCTCTATAATTGATGGAGTTCCCAACTTACGTATTGAGGCTTGA

Coding sequence (CDS)

ATGGTAGAGAATCAGACGGACAAGAAAATCAAAAGGCTGAGGTCAGACAACGGGGGAGAATATACTTATGATCCATTCCTTAAAGTATGCCGAGATGAAGGGATCGTTCGACACTTCACTGTTCCTGGTAAGCCACAACAAAATGGAGTTGCTGAGAGAATGAACCAGACATTAATAGAGAAGGTTCGATGCATATTGTCTCAAGCAGGATTGAGTAAGGCATTTTGGGCTGAGGCTCTCAGTTATGCAGTTCACTTGGTGAATCGTTTACCTGTTTCTGGAAACGGTGGGAGAACTCCGCTTGAGGTATGGTCAGGTAGTCCTGTTAGTGATTATGATAAATTACATGTGTTTGGGTGTCCTGCTTATTATCATGTGACAGACTCAAAGCTGGATCCTAGAGCAAAAAAAGCCAAGTTTATGGGCTTTAGCAAAGGTGTGAAGGGCTATAGGTTATGGTGTCCAGAAACAAGTAAGATTGTTAATAGTCGAGATGTGACATTCGATGAGTCTGGAATGTTTTTGCAGAAAATTGAAAATAATGACGAGGCATTGAAGCAGGTGGAGAAGGTGGTGTTCTCTCCTGATGTGGTTGCTCCTACTGAAGAACCTATTGACCAGGTAAATAATAACTTTGATGTCTTAGAACAGAAAGAGCAAAGTCTGGAGGAGCAAAGCCTTGTAAATGAAAGAGTGGAGGAACCTGAGTCTATCGCCAAGAATAGACCACGAAGGGTAATTCGAAAACCTGCAAGGTTTGATGATACGATAGCATATGCTTTCTCTATAATTGATGGAGTTCCCAACTTACGTATTGAGGCTTGA

Protein sequence

MVENQTDKKIKRLRSDNGGEYTYDPFLKVCRDEGIVRHFTVPGKPQQNGVAERMNQTLIEKVRCILSQAGLSKAFWAEALSYAVHLVNRLPVSGNGGRTPLEVWSGSPVSDYDKLHVFGCPAYYHVTDSKLDPRAKKAKFMGFSKGVKGYRLWCPETSKIVNSRDVTFDESGMFLQKIENNDEALKQVEKVVFSPDVVAPTEEPIDQVNNNFDVLEQKEQSLEEQSLVNERVEEPESIAKNRPRRVIRKPARFDDTIAYAFSIIDGVPNLRIEA
Homology
BLAST of CmaCh11G006340 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 165.2 bits (417), Expect = 1.0e-39
Identity = 100/244 (40.98%), Postives = 136/244 (55.74%), Query Frame = 0

Query: 1   MVENQTDKKIKRLRSDNGGEYTYDPFLKVCRDEGIVRHFTVPGKPQQNGVAERMNQTLIE 60
           +VE +T +K+KRLRSDNGGEYT   F + C   GI    TVPG PQ NGVAERMN+T++E
Sbjct: 535 LVERETGRKLKRLRSDNGGEYTSREFEEYCSSHGIRHEKTVPGTPQHNGVAERMNRTIVE 594

Query: 61  KVRCILSQAGLSKAFWAEALSYAVHLVNRLPVSGNGGRTPLEVWSGSPVSDYDKLHVFGC 120
           KVR +L  A L K+FW EA+  A +L+NR P        P  VW+   VS Y  L VFGC
Sbjct: 595 KVRSMLRMAKLPKSFWGEAVQTACYLINRSPSVPLAFEIPERVWTNKEVS-YSHLKVFGC 654

Query: 121 PAYYHVTD---SKLDPRAKKAKFMGFSKGVKGYRLWCPETSKIVNSRDVTFDESGMFLQK 180
            A+ HV     +KLD ++    F+G+     GYRLW P   K++ SRDV F ES     +
Sbjct: 655 RAFAHVPKEQRTKLDDKSIPCIFIGYGDEEFGYRLWDPVKKKVIRSRDVVFRES-----E 714

Query: 181 IENNDEALKQVEKVVFSPDVVAP--TEEPIDQVNNNFDVLEQKEQS---LEEQSLVNERV 237
           +    +  ++V+  +    V  P  +  P    +   +V EQ EQ    +E+   ++E V
Sbjct: 715 VRTAADMSEKVKNGIIPNFVTIPSTSNNPTSAESTTDEVSEQGEQPGEVIEQGEQLDEGV 772

BLAST of CmaCh11G006340 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 120.2 bits (300), Expect = 3.7e-26
Identity = 66/170 (38.82%), Postives = 95/170 (55.88%), Query Frame = 0

Query: 9   KIKRLRSDNGGEYTYDPFLKVCRDEGIVRHFTVPGKPQQNGVAERMNQTLIEKVRCILSQ 68
           K+  L  DNG EY  +   + C  +GI  H TVP  PQ NGV+ERM +T+ EK R ++S 
Sbjct: 543 KVVYLYIDNGREYLSNEMRQFCVKKGISYHLTVPHTPQLNGVSERMIRTITEKARTMVSG 602

Query: 69  AGLSKAFWAEALSYAVHLVNRLPVSG--NGGRTPLEVWSGSPVSDYDK-LHVFGCPAYYH 128
           A L K+FW EA+  A +L+NR+P     +  +TP E+W       Y K L VFG   Y H
Sbjct: 603 AKLDKSFWGEAVLTATYLINRIPSRALVDSSKTPYEMWHNK--KPYLKHLRVFGATVYVH 662

Query: 129 VTD--SKLDPRAKKAKFMGFSKGVKGYRLWCPETSKIVNSRDVTFDESGM 174
           + +   K D ++ K+ F+G+     G++LW     K + +RDV  DE+ M
Sbjct: 663 IKNKQGKFDDKSFKSIFVGYEP--NGFKLWDAVNEKFIVARDVVVDETNM 708

BLAST of CmaCh11G006340 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.6e-21
Identity = 62/174 (35.63%), Postives = 94/174 (54.02%), Query Frame = 0

Query: 1   MVENQTDKKIKRLRSDNGGEYTYDPFLKVCRDEGIVRHFT-VPGKPQQNGVAERMNQTLI 60
           +VEN+   +I  L SDNGGE+     L+    +  + HFT  P  P+ NG++ER ++ ++
Sbjct: 556 LVENRFQTRIGTLYSDNGGEFV---VLRDYLSQHGISHFTSPPHTPEHNGLSERKHRHIV 615

Query: 61  EKVRCILSQAGLSKAFWAEALSYAVHLVNRLPVSGNGGRTPLEVWSGSPVSDYDKLHVFG 120
           E    +LS A + K +W  A S AV+L+NRLP      ++P +   G P  +Y+KL VFG
Sbjct: 616 EMGLTLLSHASVPKTYWPYAFSVAVYLINRLPTPLLQLQSPFQKLFGQP-PNYEKLKVFG 675

Query: 121 CPAYYHV---TDSKLDPRAKKAKFMGFSKGVKGYRLWCPETSKIVNSRDVTFDE 171
           C  Y  +      KL+ ++K+  FMG+S     Y      T ++  SR V FDE
Sbjct: 676 CACYPWLRPYNRHKLEDKSKQCAFMGYSLTQSAYLCLHIPTGRLYTSRHVQFDE 725

BLAST of CmaCh11G006340 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 3.6e-21
Identity = 65/212 (30.66%), Postives = 106/212 (50.00%), Query Frame = 0

Query: 1   MVENQTDKKIKRLRSDNGGEYTYDPFLKVCRDEGIVRHFTVPGKPQQNGVAERMNQTLIE 60
           ++EN+   +I    SDNGGE+      +     GI    + P  P+ NG++ER ++ ++E
Sbjct: 577 LLENRFQTRIGTFYSDNGGEFV--ALWEYFSQHGISHLTSPPHTPEHNGLSERKHRHIVE 636

Query: 61  KVRCILSQAGLSKAFWAEALSYAVHLVNRLPVSGNGGRTPLEVWSGSPVSDYDKLHVFGC 120
               +LS A + K +W  A + AV+L+NRLP       +P +   G+   +YDKL VFGC
Sbjct: 637 TGLTLLSHASIPKTYWPYAFAVAVYLINRLPTPLLQLESPFQKLFGTS-PNYDKLRVFGC 696

Query: 121 PAYYHV---TDSKLDPRAKKAKFMGFSKGVKGYRLWCPETSKIVNSRDVTFDES----GM 180
             Y  +      KLD ++++  F+G+S     Y     +TS++  SR V FDE+      
Sbjct: 697 ACYPWLRPYNQHKLDDKSRQCVFLGYSLTQSAYLCLHLQTSRLYISRHVRFDENCFPFSN 756

Query: 181 FLQKIENNDEALKQVEKVVFSPDVVAPTEEPI 206
           +L  +    E  ++    V+SP    PT  P+
Sbjct: 757 YLATLSPVQEQRRE-SSCVWSPHTTLPTRTPV 784

BLAST of CmaCh11G006340 vs. ExPASy Swiss-Prot
Match: P92512 (Uncharacterized mitochondrial protein AtMg00710 OS=Arabidopsis thaliana OX=3702 GN=AtMg00710 PE=4 SV=1)

HSP 1 Score: 82.8 bits (203), Expect = 6.6e-15
Identity = 42/89 (47.19%), Postives = 54/89 (60.67%), Query Frame = 0

Query: 54  MNQTLIEKVRCILSQAGLSKAFWAEALSYAVHLVNRLPVSGNGGRTPLEVWSGSPVSDYD 113
           MN+T+IEKVR +L + GL K F A+A + AVH++N+ P +      P EVW  S V  Y 
Sbjct: 1   MNRTIIEKVRSMLCECGLPKTFRADAANTAVHIINKYPSTAINFHVPDEVWFQS-VPTYS 60

Query: 114 KLHVFGCPAYYHVTDSKLDPRAKKAKFMG 143
            L  FGC AY H  + KL PRAKK +  G
Sbjct: 61  YLRRFGCVAYIHCDEGKLKPRAKKGEEKG 88

BLAST of CmaCh11G006340 vs. TAIR 10
Match: ATMG00710.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 82.8 bits (203), Expect = 4.7e-16
Identity = 42/89 (47.19%), Postives = 54/89 (60.67%), Query Frame = 0

Query: 54  MNQTLIEKVRCILSQAGLSKAFWAEALSYAVHLVNRLPVSGNGGRTPLEVWSGSPVSDYD 113
           MN+T+IEKVR +L + GL K F A+A + AVH++N+ P +      P EVW  S V  Y 
Sbjct: 1   MNRTIIEKVRSMLCECGLPKTFRADAANTAVHIINKYPSTAINFHVPDEVWFQS-VPTYS 60

Query: 114 KLHVFGCPAYYHVTDSKLDPRAKKAKFMG 143
            L  FGC AY H  + KL PRAKK +  G
Sbjct: 61  YLRRFGCVAYIHCDEGKLKPRAKKGEEKG 88

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P109781.0e-3940.98Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P041463.7e-2638.82Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
Q9ZT941.6e-2135.63Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Q94HW23.6e-2130.66Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
P925126.6e-1547.19Uncharacterized mitochondrial protein AtMg00710 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
ATMG00710.14.7e-1647.19Polynucleotidyl transferase, ribonuclease H-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 205..225
NoneNo IPR availablePANTHERPTHR45895FAMILY NOT NAMEDcoord: 2..178
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 2..111
e-value: 4.1E-26
score: 93.7
IPR001584Integrase, catalytic corePROSITEPS50994INTEGRASEcoord: 1..108
score: 19.191778
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 8..108

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G006340.1CmaCh11G006340.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0015074 DNA integration
molecular_function GO:0016788 hydrolase activity, acting on ester bonds
molecular_function GO:0046872 metal ion binding
molecular_function GO:0003676 nucleic acid binding