CmaCh10G006780 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G006780
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description(1->3)-beta-glucan endohydrolase
LocationCma_Chr10: 3078604 .. 3080556 (+)
RNA-Seq ExpressionCmaCh10G006780
SyntenyCmaCh10G006780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAATTTGGTTACAGGCCATTGCCAGGGAAGTAAAGTTGGTGTTTGCTATGGAAGGAACGCAGACGACCTCCCAACACCTAATAAAGTGGCTCAGCTGGTCCAACTTCATAACATCAAATATCTTAGAATCTATGATTCAAACATTCAAGTTCTCAAGGCCTTTGCCAACACTGGTGTTGAACTTATGATTGGGGTTCCGAATTCAGATTTGCTGTCTTTCGCTCAGTTCCAGTCTAATGTAGACTCTTGGCTGAAGAACAGCATACTTCCTTATTACCCAGCTTCGAAAATAACGTACATAACGGTTGGTGCCGAGGTCACCGAGAGCCCCAACAATGTGTCTTCCTTGGTGGTGCCTGCCATGAAAAATGTACTCACTGGTCTCAAAAAGGCTGGTTTGCACAAGAAGATAAAGGTTTCTAGCACTCATTCCCTTGGCGTTTTGTCTCGGTCGTTTCCTCCTTCGGCTGGTGCTTTTAGTAGTAACTATGCATTTTTCCTGAAGCCATTGTTGGAGTTTCTTGCTGACAACCAGTCTCCCTTTATGATCAATATCTATCCTTATTATGCTTACAGAGAATCCCCCAGCAACGTGTCGCTAGACTATGCTCTTTTCGAGTCATCATCCGAAGTTATTGATCCGAATACTGGTCTGCTTTACACGAACATGTTTGATGCTCAGATTGATGCTCTTTATTTTGCTTTGATGGCTCTAAATTTTAGAACAATTAGAGTGATGGTCACAGAAACAGGCTGGCCATCTAAAGGGTCGCCAAAGGAGACGTCTGCGACTCCCGACAATGCTCAAACTTACAACACGAATCTTATCCGCCATGTTATCAATAATACAGGGACACCTGCAAGGCCTGGAGAGAAACTAGATGTATATATCTTCTCTTTGTTCAATGAAAACAGGAAGCCGGGGTTGGATTCTGAAAGGAACTGGGGATTGTTCTATCCAGACCAGACCAGTGTCTACAACTTGGACTTCACGGGAAAGGGCGCGGTTGACGCGTCTACACAGGCTAATAGTACCAGCTCGAATAGAACTGCCTGGTGCATAGCTTCAAGCAAGGCTTCTGATATGGACTTGCAAAACGCCTTGGATTGGGCATGTGGTTCAGGTAATGTCGATTGCACAGCCATTCAGCCAAGCCAGCCTTGTTTCGAACCAGATACTCTGGTTTCCCATGCATCTTATGCATTTAACAGCTATTTCCAGCAAAATGGAGCCACTGATGTTGCTTGTGGATTTGGAGGGAATGGAGTTGAAGTTAACAAGGACCCAAGTAAGTCACGATCGCATTTCCGGAGCATACGTCATATACTTCAGTGTTGTACCTCCGTTACACGGTTAACACAATTGAAATCTTCGTTTTGTGTTCTAATCTCTGTTTCGTGTTACTAGTTGAAAGACTTTTTAGATAAAATGTTAAAACAAGTGTTCCTAACTCGAATTTGTACGTTGACTTTGCAGGCTATGATAATTGCTTGTATGCAACTATTGGGTAAGCCTTTACTTTTGTTTCCAAGTTCATAGATTTTGATTTCCAGTTCCTGATGTTCTTTGCCTTTCCCGTGTTTCTTACTGTCAAGCAGGAAGAACAAGACGATCTCTTCCAGTAACACGACGGCAATATCCTCAACTTCCTCATCTTCTACATCAAGGATTGAAGTTTCTGCAGGTTTACCTATTATCATGGCCTTCATATCATATTTTTTGTAAATTTCATGAGAAGGGTCGGCCAGATTTTGCTATGCAAATCGTGTATGTAGTGTCTGTATGATAAGAATGTGTCTATTTATAAGGAGGTCAATGAAGATAACGGTCTGAAGTCGATTGAATTGGTGAAAATCGTACTGAATAAGTATTGCACTTACAAAAATTAAAAAAAAAGAAGATATCTCTTTATAAGTGTAGAAACACTTATAAAACCGTCCAAGAGTATGC

mRNA sequence

ATGAATTTGGTTACAGGCCATTGCCAGGGAAGTAAAGTTGGTGTTTGCTATGGAAGGAACGCAGACGACCTCCCAACACCTAATAAAGTGGCTCAGCTGGTCCAACTTCATAACATCAAATATCTTAGAATCTATGATTCAAACATTCAAGTTCTCAAGGCCTTTGCCAACACTGGTGTTGAACTTATGATTGGGGTTCCGAATTCAGATTTGCTGTCTTTCGCTCAGTTCCAGTCTAATGTAGACTCTTGGCTGAAGAACAGCATACTTCCTTATTACCCAGCTTCGAAAATAACGTACATAACGGTTGGTGCCGAGGTCACCGAGAGCCCCAACAATGTGTCTTCCTTGGTGGTGCCTGCCATGAAAAATGTACTCACTGGTCTCAAAAAGGCTGGTTTGCACAAGAAGATAAAGGTTTCTAGCACTCATTCCCTTGGCGTTTTGTCTCGGTCGTTTCCTCCTTCGGCTGGTGCTTTTAGTAGTAACTATGCATTTTTCCTGAAGCCATTGTTGGAGTTTCTTGCTGACAACCAGTCTCCCTTTATGATCAATATCTATCCTTATTATGCTTACAGAGAATCCCCCAGCAACGTGTCGCTAGACTATGCTCTTTTCGAGTCATCATCCGAAGTTATTGATCCGAATACTGGTCTGCTTTACACGAACATGTTTGATGCTCAGATTGATGCTCTTTATTTTGCTTTGATGGCTCTAAATTTTAGAACAATTAGAGTGATGGTCACAGAAACAGGCTGGCCATCTAAAGGGTCGCCAAAGGAGACGTCTGCGACTCCCGACAATGCTCAAACTTACAACACGAATCTTATCCGCCATGTTATCAATAATACAGGGACACCTGCAAGGCCTGGAGAGAAACTAGATGTATATATCTTCTCTTTGTTCAATGAAAACAGGAAGCCGGGGTTGGATTCTGAAAGGAACTGGGGATTGTTCTATCCAGACCAGACCAGTGTCTACAACTTGGACTTCACGGGAAAGGGCGCGGTTGACGCGTCTACACAGGCTAATAGTACCAGCTCGAATAGAACTGCCTGGTGCATAGCTTCAAGCAAGGCTTCTGATATGGACTTGCAAAACGCCTTGGATTGGGCATGTGGTTCAGGTAATGTCGATTGCACAGCCATTCAGCCAAGCCAGCCTTGTTTCGAACCAGATACTCTGGTTTCCCATGCATCTTATGCATTTAACAGCTATTTCCAGCAAAATGGAGCCACTGATGTTGCTTGTGGATTTGGAGGGAATGGAGTTGAAGTTAACAAGGACCCAAGCTATGATAATTGCTTGTATGCAACTATTGGGAAGAACAAGACGATCTCTTCCAGTAACACGACGGCAATATCCTCAACTTCCTCATCTTCTACATCAAGGATTGAAGTTTCTGCAGGTTTACCTATTATCATGGCCTTCATATCATATTTTTTGTAAATTTCATGAGAAGGGTCGGCCAGATTTTGCTATGCAAATCGTGTATGTAGTGTCTGTATGATAAGAATGTGTCTATTTATAAGGAGGTCAATGAAGATAACGGTCTGAAGTCGATTGAATTGGTGAAAATCGTACTGAATAAGTATTGCACTTACAAAAATTAAAAAAAAAGAAGATATCTCTTTATAAGTGTAGAAACACTTATAAAACCGTCCAAGAGTATGC

Coding sequence (CDS)

ATGAATTTGGTTACAGGCCATTGCCAGGGAAGTAAAGTTGGTGTTTGCTATGGAAGGAACGCAGACGACCTCCCAACACCTAATAAAGTGGCTCAGCTGGTCCAACTTCATAACATCAAATATCTTAGAATCTATGATTCAAACATTCAAGTTCTCAAGGCCTTTGCCAACACTGGTGTTGAACTTATGATTGGGGTTCCGAATTCAGATTTGCTGTCTTTCGCTCAGTTCCAGTCTAATGTAGACTCTTGGCTGAAGAACAGCATACTTCCTTATTACCCAGCTTCGAAAATAACGTACATAACGGTTGGTGCCGAGGTCACCGAGAGCCCCAACAATGTGTCTTCCTTGGTGGTGCCTGCCATGAAAAATGTACTCACTGGTCTCAAAAAGGCTGGTTTGCACAAGAAGATAAAGGTTTCTAGCACTCATTCCCTTGGCGTTTTGTCTCGGTCGTTTCCTCCTTCGGCTGGTGCTTTTAGTAGTAACTATGCATTTTTCCTGAAGCCATTGTTGGAGTTTCTTGCTGACAACCAGTCTCCCTTTATGATCAATATCTATCCTTATTATGCTTACAGAGAATCCCCCAGCAACGTGTCGCTAGACTATGCTCTTTTCGAGTCATCATCCGAAGTTATTGATCCGAATACTGGTCTGCTTTACACGAACATGTTTGATGCTCAGATTGATGCTCTTTATTTTGCTTTGATGGCTCTAAATTTTAGAACAATTAGAGTGATGGTCACAGAAACAGGCTGGCCATCTAAAGGGTCGCCAAAGGAGACGTCTGCGACTCCCGACAATGCTCAAACTTACAACACGAATCTTATCCGCCATGTTATCAATAATACAGGGACACCTGCAAGGCCTGGAGAGAAACTAGATGTATATATCTTCTCTTTGTTCAATGAAAACAGGAAGCCGGGGTTGGATTCTGAAAGGAACTGGGGATTGTTCTATCCAGACCAGACCAGTGTCTACAACTTGGACTTCACGGGAAAGGGCGCGGTTGACGCGTCTACACAGGCTAATAGTACCAGCTCGAATAGAACTGCCTGGTGCATAGCTTCAAGCAAGGCTTCTGATATGGACTTGCAAAACGCCTTGGATTGGGCATGTGGTTCAGGTAATGTCGATTGCACAGCCATTCAGCCAAGCCAGCCTTGTTTCGAACCAGATACTCTGGTTTCCCATGCATCTTATGCATTTAACAGCTATTTCCAGCAAAATGGAGCCACTGATGTTGCTTGTGGATTTGGAGGGAATGGAGTTGAAGTTAACAAGGACCCAAGCTATGATAATTGCTTGTATGCAACTATTGGGAAGAACAAGACGATCTCTTCCAGTAACACGACGGCAATATCCTCAACTTCCTCATCTTCTACATCAAGGATTGAAGTTTCTGCAGGTTTACCTATTATCATGGCCTTCATATCATATTTTTTGTAA

Protein sequence

MNLVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASDMDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL
Homology
BLAST of CmaCh10G006780 vs. ExPASy Swiss-Prot
Match: Q9FJU9 (Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590 PE=2 SV=1)

HSP 1 Score: 694.9 bits (1792), Expect = 6.5e-199
Identity = 341/460 (74.13%), Postives = 401/460 (87.17%), Query Frame = 0

Query: 8   CQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVP 67
           C G KVGVCYGR+ADDLPTP+KV QL+Q HNIKY+RIYD N QVLKAF NT +ELMIGVP
Sbjct: 21  CYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVP 80

Query: 68  NSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPN-NVSSLVVPAMKNVL 127
           NSDL +F+Q QSNVD+WLKNS+LPYYP +KITYITVGAE T+ P+ N SS VVPAM+NVL
Sbjct: 81  NSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVL 140

Query: 128 TGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINI 187
           T L+K GL ++IKVS+T SLG+LSRSFPPSAGAF+S+YA+FL+P+LEFLA+N+SPFMI++
Sbjct: 141 TALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDL 200

Query: 188 YPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRV 247
           YPYYAYR+SP+NVSLDY LFESSSEVIDPNTGLLY NMFDAQ+DALY+AL ALNFRTI++
Sbjct: 201 YPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKI 260

Query: 248 MVTETGWPSKGSPKE-TSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNEN 307
           MVTETGWP+KGSPKE  +A+ DNA+TYN+N+IRHV+ N GTPA+PGE ++VYIFSLFNEN
Sbjct: 261 MVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNEN 320

Query: 308 RKPGLDSERNWGLFYPDQTSVYNLDFTGK-GAVDASTQANSTSSNRTAWCIASSKASDMD 367
           RK GLDSERNWGLFYPDQTSVY LDFTGK     +++   ++S +  +WCIASSKAS+ D
Sbjct: 321 RKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSSGSSNSWCIASSKASERD 380

Query: 368 LQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGV 427
           L+ ALDWACG GNVDCTAIQPSQPCF+PDTLVSHAS+ FNSYFQQN ATDVAC FGG GV
Sbjct: 381 LKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGV 440

Query: 428 EVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSR 465
           +VNKDPSYD C+Y T G NKT  ++N TA+  TSS+ST R
Sbjct: 441 KVNKDPSYDKCIYITAGGNKT-KATNATAL--TSSASTPR 477

BLAST of CmaCh10G006780 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 629.8 bits (1623), Expect = 2.6e-179
Identity = 306/487 (62.83%), Postives = 393/487 (80.70%), Query Frame = 0

Query: 11  SKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPNSD 70
           SK+G+CYGRNAD+LP+PN+V++L+Q  NIK++RIYD+NI VLKAFANTG+ELMIGVPN+D
Sbjct: 25  SKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNAD 84

Query: 71  LLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVLTGLK 130
           LL+FAQFQSNVD+WL N+ILPYYP++KIT I+VG EVTE+P+N + LV+PAM+N+ T LK
Sbjct: 85  LLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTALK 144

Query: 131 KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINIYPYY 190
           K+GL KKIK+SS+HSL +LSRSFPPS+ +FS  ++ FLKP+LEFL +N+SPFMI++YPYY
Sbjct: 145 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYY 204

Query: 191 AYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRVMVTE 250
           AYR+S   V L+YALFESSS+V+DP TGLLY+NMFDAQ+DA+YFAL A++F+T++VMVTE
Sbjct: 205 AYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTE 264

Query: 251 TGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNENRKPGL 310
           +GWPSKGSPKET+ATP+NA  YNTNLIRHVI + GTPA+PGE++DVY+FSLFNENRKPG+
Sbjct: 265 SGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPGI 324

Query: 311 DSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRT------------------- 370
           +SERNWG+FY + T+VY LDFTG+     S   ++T ++ +                   
Sbjct: 325 ESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGG 384

Query: 371 -----AWCIASSKASDMDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSY 430
                 WCIASS+AS  +LQ ALDWACG GNVDC+A+QP QPCFEPDT++SHASYAFN+Y
Sbjct: 385 GGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTY 444

Query: 431 FQQNGATDVACGFGGNGVEVNKDPSYDNCLY----ATIGKNKTIS---SSNTTAISSTSS 467
           +QQ+GA+ + C F G  VEV+KDPSY NCLY    AT G N+T++   + N TAI S  +
Sbjct: 445 YQQSGASSIDCSFNGASVEVDKDPSYGNCLYMIAPATDGFNRTMAGNITGNITAIDSPLA 504

BLAST of CmaCh10G006780 vs. ExPASy Swiss-Prot
Match: Q9ZU91 (Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 PE=2 SV=2)

HSP 1 Score: 395.2 bits (1014), Expect = 1.1e-108
Identity = 201/458 (43.89%), Postives = 291/458 (63.54%), Query Frame = 0

Query: 9   QGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPN 68
           Q S +GV  G    ++P+P +V  L++  NI  +R+YD++  +L AFA+TGV+++I VPN
Sbjct: 19  QDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFAHTGVQVIISVPN 78

Query: 69  SDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVLTG 128
             LL  +Q  +   +W+  ++  YYPA+ IT I VG+EV  S  N +S++V A+K +   
Sbjct: 79  DQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAASVLVSALKYIQAA 138

Query: 129 LKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINIYP 188
           L  A L ++IKVS+ HS  ++  SFPPS   F+  +   + PLL+FL    SP ++N+YP
Sbjct: 139 LVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLLKFLQSTGSPLLLNVYP 198

Query: 189 YYAYRESPSNVSLDYALF---ESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIR 248
           Y+ Y +S   + LDYALF   +++ E +D NT L YTN+FDA +DA YFA+  LNF  I 
Sbjct: 199 YFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAAYFAMSYLNFTNIP 258

Query: 249 VMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNEN 308
           ++VTE+GWPSKG P E  AT +NA TYN+NLI+HVIN TGTP  PG  +  YI+ L+NE+
Sbjct: 259 IVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGTAVTTYIYELYNED 318

Query: 309 RKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASDMDL 368
            +PG  SE+NWGLFY + T VY L   G GA+ A+   N T      +CIA  K     L
Sbjct: 319 TRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQT------FCIAKEKVDRKML 378

Query: 369 QNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVE 428
           Q ALDWACG G VDC+A+   + C+EPD +V+H++YAFN+Y+Q+ G    +C F G    
Sbjct: 379 QAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATV 438

Query: 429 VNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTS 464
              DPS   C++    K+     +NT+A++ +++S+TS
Sbjct: 439 TTTDPSRGTCVFPGSAKSNQTLGNNTSALAPSANSTTS 470

BLAST of CmaCh10G006780 vs. ExPASy Swiss-Prot
Match: Q9C7U5 (Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 PE=2 SV=2)

HSP 1 Score: 371.7 bits (953), Expect = 1.3e-101
Identity = 198/458 (43.23%), Postives = 284/458 (62.01%), Query Frame = 0

Query: 9   QGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPN 68
           +GS +GV  G +  D+P P +V  L++   I+++R+Y+++  +L A ANTG++++I +PN
Sbjct: 28  EGSYIGVNIGTDLSDMPHPTQVVALLKAQEIRHIRLYNADPGLLIALANTGIKVIISIPN 87

Query: 69  SDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVLTG 128
             LL   Q  S   +W+K +++ +YPA+ IT ++VG+EV  S +N + ++V A+KNV   
Sbjct: 88  DQLLGIGQSNSTAANWVKRNVIAHYPATMITAVSVGSEVLTSLSNAAPVLVSAIKNVHAA 147

Query: 129 LKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINIYP 188
           L  A L K IKVS+  S  ++   FPPS   F+ +    + PLL FL    S  M+N+YP
Sbjct: 148 LLSANLDKLIKVSTPLSTSLILDPFPPSQAFFNRSLNAVIVPLLSFLQSTNSYLMVNVYP 207

Query: 189 YYAYRESPSNVSLDYALFE---SSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIR 248
           Y  Y +S   + LDYALF+    + E +D NT + Y+N FDA +DA YFA+  LNF  I 
Sbjct: 208 YIDYMQSNGVIPLDYALFKPIPPNKEAVDANTLVRYSNAFDAMVDATYFAMAFLNFTNIP 267

Query: 249 VMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNEN 308
           V+VTE+GWPSKG   E  AT DNA TYN+NLIRHV+N TGTP RPG  +  YI+ L+NE+
Sbjct: 268 VLVTESGWPSKGETNEPDATLDNANTYNSNLIRHVLNKTGTPKRPGIAVSTYIYELYNED 327

Query: 309 RKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASDMDL 368
            K GL SE+NWGLF  +   VY L  T  G+V A+   N T      +C A   A    L
Sbjct: 328 TKAGL-SEKNWGLFNANGEPVYVLRLTNSGSVLANDTTNQT------YCTAREGADTKML 387

Query: 369 QNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVE 428
           Q ALDWACG G +DC+ I+  + C+EPD +V+HA+YAF++Y+ Q G    AC F G    
Sbjct: 388 QAALDWACGPGKIDCSPIKQGETCYEPDNVVAHANYAFDTYYHQTGNNPDACNFNGVASI 447

Query: 429 VNKDPSYDNCLYA-TIGKNKTISSSNTTAISSTSSSST 463
              DPS+  C++A + G  +  +S N TA S+ S++S+
Sbjct: 448 TTTDPSHGTCVFAGSRGNGRNGTSVNITAPSANSTTSS 478

BLAST of CmaCh10G006780 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 365.2 bits (936), Expect = 1.2e-99
Identity = 195/477 (40.88%), Postives = 297/477 (62.26%), Query Frame = 0

Query: 13  VGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPNSDLL 72
           +GV YG+ AD+LP P++  +L+Q  +I+ +R+Y ++  ++KA A TGV ++IG  N D+ 
Sbjct: 26  IGVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVP 85

Query: 73  SFAQFQSNVDSWLKNSILPYYPASKITYITVGAEV--TESPNNVSSLVVPAMKNVLTGLK 132
           S A   +    W+ +++LP+YPASKI  ITVG E+  +  PN V+ L +PAM+NV   L+
Sbjct: 86  SLASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQL-LPAMQNVQKALE 145

Query: 133 KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINIYPYY 192
              L  KIKVS+ +S+ VL  S PPS+G+F++ Y   LK +L+FL+D  SPF IN YP++
Sbjct: 146 AVSLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFF 205

Query: 193 AYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRVMVTE 252
           AY+  P   +L + LFE ++  +D  TG+ YTNMFDAQ+DA++ AL ++ F  + ++V E
Sbjct: 206 AYQSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAE 265

Query: 253 TGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNENRKPGL 312
           TGW S+G   E  A+ DNA+ YN NLI H+ +  GTP  PG+ +D YIF+L++EN KPG 
Sbjct: 266 TGWASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGP 325

Query: 313 DSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASDMDLQNALD 372
            SER +GLF  D + VY++   G     +S+Q  S     + WC+    A++ +LQ +LD
Sbjct: 326 SSERAFGLFKTDLSMVYDV---GLAKSSSSSQTPSGKVTSSGWCVPKKGATNEELQASLD 385

Query: 373 WACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVEVNKDP 432
           WACG G +DC AIQP   CFEP+ +VSHA+YA N YFQ++      C F       +++P
Sbjct: 386 WACGHG-IDCGAIQPGGACFEPNNVVSHAAYAMNMYFQKSPKQPTDCDFSKTATVTSQNP 445

Query: 433 SYDNCLY--------ATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFIS 480
           SY+NC+Y           G +K + +   ++      +  +  +VS+ L  ++ F+S
Sbjct: 446 SYNNCVYPGGGGGGGGGGGGSKAVMNKYVSSDKVEKKNGATEPKVSSSLSFLLIFLS 497

BLAST of CmaCh10G006780 vs. ExPASy TrEMBL
Match: A0A6J1JF22 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485174 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.0e-272
Identity = 477/480 (99.38%), Postives = 479/480 (99.79%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++ GHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL
Sbjct: 24  MLLGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 83

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM
Sbjct: 84  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 143

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
           KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF
Sbjct: 144 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 203

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 204 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 263

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF
Sbjct: 264 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 323

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD
Sbjct: 324 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 383

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 384 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 443

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 482
           GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL
Sbjct: 444 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 503

BLAST of CmaCh10G006780 vs. ExPASy TrEMBL
Match: A0A6J1H8Q8 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461577 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 8.9e-268
Identity = 468/480 (97.50%), Postives = 475/480 (98.96%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++  HCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL
Sbjct: 24  MLLDHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 83

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIGVPNSDLL+FAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVS+LVVPAM
Sbjct: 84  MIGVPNSDLLAFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSALVVPAM 143

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
           KNVLTGL+KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF
Sbjct: 144 KNVLTGLRKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 203

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 204 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 263

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGE+LDVYIFSLF
Sbjct: 264 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLF 323

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVD STQANSTSSN TAWCIASSKASD
Sbjct: 324 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDMSTQANSTSSNGTAWCIASSKASD 383

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 384 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 443

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 482
           GV+VNKDPSYDNCLYATIGKNKTISS NTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL
Sbjct: 444 GVKVNKDPSYDNCLYATIGKNKTISSGNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 503

BLAST of CmaCh10G006780 vs. ExPASy TrEMBL
Match: A0A0A0M1T0 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G575070 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.5e-254
Identity = 442/480 (92.08%), Postives = 462/480 (96.25%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++ GHCQGS VGVCYGRNADDLPTPNKVAQLV+LHNIKY+RIYDSNIQVLKAFANTGVEL
Sbjct: 19  ILLGHCQGSNVGVCYGRNADDLPTPNKVAQLVKLHNIKYIRIYDSNIQVLKAFANTGVEL 78

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIGVPNSDLL FAQFQSNVD+WLKNSILPYYPA+KITYITVGAEVTESPNNVS+LVVPAM
Sbjct: 79  MIGVPNSDLLPFAQFQSNVDTWLKNSILPYYPATKITYITVGAEVTESPNNVSALVVPAM 138

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
            NVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPS GAFSSNYA FLKPLLEFLA+NQSPF
Sbjct: 139 NNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSVGAFSSNYASFLKPLLEFLAENQSPF 198

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESP+NVSLDYALFESS+EVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 199 MINIYPYYAYRESPNNVSLDYALFESSNEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 258

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGE+LDVYIFSLF
Sbjct: 259 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLF 318

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDFTGK  VD + QANST+SN TAWCIAS+KASD
Sbjct: 319 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKSVVDMTAQANSTASNGTAWCIASTKASD 378

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACGSGNVDCT IQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 379 MDLQNALDWACGSGNVDCTPIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 438

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 482
           GV+VN+DPSYD CLYAT GKNKTISSSNTTAISSTSSSS+SRIE SAGL +++ FIS FL
Sbjct: 439 GVKVNQDPSYDTCLYATTGKNKTISSSNTTAISSTSSSSSSRIEFSAGLLVMLIFISCFL 498

BLAST of CmaCh10G006780 vs. ExPASy TrEMBL
Match: A0A1S3BPG3 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492273 PE=3 SV=1)

HSP 1 Score: 885.2 bits (2286), Expect = 1.2e-253
Identity = 440/480 (91.67%), Postives = 461/480 (96.04%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++ GHCQGS VGVCYGRNADDLPTPNKVAQLV+LHNIKY+RIYDSNIQVLKAFANTGVEL
Sbjct: 19  ILLGHCQGSNVGVCYGRNADDLPTPNKVAQLVKLHNIKYIRIYDSNIQVLKAFANTGVEL 78

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIGVPNSDLL FAQFQSNVD+WLKNSILPYYPA+KITYITVGAEVTESPNNVS+LVVPAM
Sbjct: 79  MIGVPNSDLLPFAQFQSNVDTWLKNSILPYYPATKITYITVGAEVTESPNNVSALVVPAM 138

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
            NVL GLKKAGLHKKIKVSSTHSLGVLSRSFPPS GAFSSNYA FLKPLLEFLA+NQSPF
Sbjct: 139 NNVLAGLKKAGLHKKIKVSSTHSLGVLSRSFPPSVGAFSSNYASFLKPLLEFLAENQSPF 198

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESP+NVSLDYALFESS+EVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 199 MINIYPYYAYRESPNNVSLDYALFESSNEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 258

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGE+LDVYIFSLF
Sbjct: 259 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLF 318

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDFTGK  VD + QAN+T+SN TAWCIASSKASD
Sbjct: 319 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKSVVDMTAQANNTASNGTAWCIASSKASD 378

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACGSGNVDCT IQPSQPCFEPDTL+SHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 379 MDLQNALDWACGSGNVDCTPIQPSQPCFEPDTLLSHASYAFNSYFQQNGATDVACGFGGN 438

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 482
           GV VN+DPSYDNCLYAT GKNKTISSSNTTAISSTSSSS+SRI+VS GL +++ FIS FL
Sbjct: 439 GVRVNQDPSYDNCLYATAGKNKTISSSNTTAISSTSSSSSSRIKVSGGLLVMLIFISCFL 498

BLAST of CmaCh10G006780 vs. ExPASy TrEMBL
Match: A0A6J1CTD8 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111014572 PE=3 SV=1)

HSP 1 Score: 884.4 bits (2284), Expect = 2.1e-253
Identity = 443/481 (92.10%), Postives = 463/481 (96.26%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++ GHCQGS VGVCYGRNADDLPTPNKVAQLVQLHNIKYLR+YDSNIQVLKAFANTGVEL
Sbjct: 18  ILLGHCQGSNVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRLYDSNIQVLKAFANTGVEL 77

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIG+PNSDLL F+QFQSNVD+WLKNSILPYYPA+KITYITVGAEVTESPNNVS+LVVPAM
Sbjct: 78  MIGIPNSDLLPFSQFQSNVDTWLKNSILPYYPAAKITYITVGAEVTESPNNVSALVVPAM 137

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
            NVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLA+NQSPF
Sbjct: 138 NNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLAENQSPF 197

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 198 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 257

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKET+ATPDNAQTYNTNLI HVINNTGTPARPGE+LDVYIFSLF
Sbjct: 258 TIRVMVTETGWPSKGSPKETAATPDNAQTYNTNLIHHVINNTGTPARPGEELDVYIFSLF 317

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDF G+GAVD +TQAN T SN TAWCIASSKASD
Sbjct: 318 NENRKPGLDSERNWGLFYPDQTSVYNLDFAGRGAVDMTTQANVTGSNGTAWCIASSKASD 377

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACG GNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 378 MDLQNALDWACGPGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 437

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSR-IEVSAGLPIIMAFISYF 482
           GV+V+KDPSYDNCLY T GKNKT+ SSNTTAISSTSSSS+S  IE SAGL +++AFISYF
Sbjct: 438 GVKVDKDPSYDNCLYETTGKNKTMPSSNTTAISSTSSSSSSTGIEASAGL-LMLAFISYF 497

BLAST of CmaCh10G006780 vs. NCBI nr
Match: XP_022987696.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita maxima])

HSP 1 Score: 947.6 bits (2448), Expect = 4.2e-272
Identity = 477/480 (99.38%), Postives = 479/480 (99.79%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++ GHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL
Sbjct: 24  MLLGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 83

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM
Sbjct: 84  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 143

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
           KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF
Sbjct: 144 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 203

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 204 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 263

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF
Sbjct: 264 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 323

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD
Sbjct: 324 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 383

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 384 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 443

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 482
           GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL
Sbjct: 444 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 503

BLAST of CmaCh10G006780 vs. NCBI nr
Match: XP_023516959.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 937.9 bits (2423), Expect = 3.3e-269
Identity = 470/480 (97.92%), Postives = 477/480 (99.38%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++ GHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL
Sbjct: 24  VLLGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 83

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIG+PNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVS+LVVPAM
Sbjct: 84  MIGIPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSALVVPAM 143

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
           KNVLTGL+KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF
Sbjct: 144 KNVLTGLRKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 203

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 204 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 263

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGE+LDVYIFSLF
Sbjct: 264 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLF 323

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVD STQANSTSSNRTAWCIASSKASD
Sbjct: 324 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDMSTQANSTSSNRTAWCIASSKASD 383

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 384 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 443

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 482
           GV+VNKDPSYDNCLYATIGKNKTISS NTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL
Sbjct: 444 GVKVNKDPSYDNCLYATIGKNKTISSGNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 503

BLAST of CmaCh10G006780 vs. NCBI nr
Match: XP_022960916.1 (glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata] >XP_022960917.1 glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata])

HSP 1 Score: 932.2 bits (2408), Expect = 1.8e-267
Identity = 468/480 (97.50%), Postives = 475/480 (98.96%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++  HCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL
Sbjct: 24  MLLDHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 83

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIGVPNSDLL+FAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVS+LVVPAM
Sbjct: 84  MIGVPNSDLLAFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSALVVPAM 143

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
           KNVLTGL+KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF
Sbjct: 144 KNVLTGLRKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 203

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 204 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 263

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGE+LDVYIFSLF
Sbjct: 264 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLF 323

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVD STQANSTSSN TAWCIASSKASD
Sbjct: 324 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDMSTQANSTSSNGTAWCIASSKASD 383

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 384 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 443

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 482
           GV+VNKDPSYDNCLYATIGKNKTISS NTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL
Sbjct: 444 GVKVNKDPSYDNCLYATIGKNKTISSGNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 503

BLAST of CmaCh10G006780 vs. NCBI nr
Match: KAG7023623.1 (Glucan endo-1,3-beta-glucosidase 13 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 931.8 bits (2407), Expect = 2.4e-267
Identity = 467/480 (97.29%), Postives = 475/480 (98.96%), Query Frame = 0

Query: 3   LVTGHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 62
           ++  HCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL
Sbjct: 24  MLLDHCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVEL 83

Query: 63  MIGVPNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAM 122
           MIGVPNSDLL+FAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVS+LVVPAM
Sbjct: 84  MIGVPNSDLLAFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSALVVPAM 143

Query: 123 KNVLTGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 182
           KNVLTGL+KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF
Sbjct: 144 KNVLTGLRKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPF 203

Query: 183 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 242
           MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR
Sbjct: 204 MINIYPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFR 263

Query: 243 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLF 302
           TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGE+LDVYIFSLF
Sbjct: 264 TIRVMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLF 323

Query: 303 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASD 362
           NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVD STQANSTSSN TAWCIASSKASD
Sbjct: 324 NENRKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDMSTQANSTSSNGTAWCIASSKASD 383

Query: 363 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 422
           MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN
Sbjct: 384 MDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGN 443

Query: 423 GVEVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 482
           GV+VNKDPSYDNCLYAT+GKNKTISS NTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL
Sbjct: 444 GVKVNKDPSYDNCLYATVGKNKTISSGNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 503

BLAST of CmaCh10G006780 vs. NCBI nr
Match: KAG6589961.1 (Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 931.0 bits (2405), Expect = 4.1e-267
Identity = 467/476 (98.11%), Postives = 473/476 (99.37%), Query Frame = 0

Query: 7   HCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGV 66
           HCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGV
Sbjct: 28  HCQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGV 87

Query: 67  PNSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVL 126
           PNSDLL+FAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVS+LVVPAMKNVL
Sbjct: 88  PNSDLLAFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSALVVPAMKNVL 147

Query: 127 TGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINI 186
           TGL+KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINI
Sbjct: 148 TGLRKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINI 207

Query: 187 YPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRV 246
           YPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRV
Sbjct: 208 YPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRV 267

Query: 247 MVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNENR 306
           MVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGE+LDVYIFSLFNENR
Sbjct: 268 MVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEELDVYIFSLFNENR 327

Query: 307 KPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASDMDLQ 366
           KPGLDSERNWGLFYPDQTSVYNLDFTGKGAVD STQANSTSSN TAWCIASSKASDMDLQ
Sbjct: 328 KPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDMSTQANSTSSNGTAWCIASSKASDMDLQ 387

Query: 367 NALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVEV 426
           NALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGV+V
Sbjct: 388 NALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVKV 447

Query: 427 NKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 483
           NKDPSYDNCLYAT+GKNKTISS NTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL
Sbjct: 448 NKDPSYDNCLYATVGKNKTISSGNTTAISSTSSSSTSRIEVSAGLPIIMAFISYFL 503

BLAST of CmaCh10G006780 vs. TAIR 10
Match: AT5G56590.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 694.9 bits (1792), Expect = 4.6e-200
Identity = 341/460 (74.13%), Postives = 401/460 (87.17%), Query Frame = 0

Query: 8   CQGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVP 67
           C G KVGVCYGR+ADDLPTP+KV QL+Q HNIKY+RIYD N QVLKAF NT +ELMIGVP
Sbjct: 21  CYGGKVGVCYGRSADDLPTPSKVVQLIQQHNIKYVRIYDYNSQVLKAFGNTSIELMIGVP 80

Query: 68  NSDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPN-NVSSLVVPAMKNVL 127
           NSDL +F+Q QSNVD+WLKNS+LPYYP +KITYITVGAE T+ P+ N SS VVPAM+NVL
Sbjct: 81  NSDLNAFSQSQSNVDTWLKNSVLPYYPTTKITYITVGAESTDDPHINASSFVVPAMQNVL 140

Query: 128 TGLKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINI 187
           T L+K GL ++IKVS+T SLG+LSRSFPPSAGAF+S+YA+FL+P+LEFLA+N+SPFMI++
Sbjct: 141 TALRKVGLSRRIKVSTTLSLGILSRSFPPSAGAFNSSYAYFLRPMLEFLAENKSPFMIDL 200

Query: 188 YPYYAYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRV 247
           YPYYAYR+SP+NVSLDY LFESSSEVIDPNTGLLY NMFDAQ+DALY+AL ALNFRTI++
Sbjct: 201 YPYYAYRDSPNNVSLDYVLFESSSEVIDPNTGLLYKNMFDAQVDALYYALTALNFRTIKI 260

Query: 248 MVTETGWPSKGSPKE-TSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNEN 307
           MVTETGWP+KGSPKE  +A+ DNA+TYN+N+IRHV+ N GTPA+PGE ++VYIFSLFNEN
Sbjct: 261 MVTETGWPTKGSPKEKAAASSDNAETYNSNIIRHVVTNQGTPAKPGEAMNVYIFSLFNEN 320

Query: 308 RKPGLDSERNWGLFYPDQTSVYNLDFTGK-GAVDASTQANSTSSNRTAWCIASSKASDMD 367
           RK GLDSERNWGLFYPDQTSVY LDFTGK     +++   ++S +  +WCIASSKAS+ D
Sbjct: 321 RKAGLDSERNWGLFYPDQTSVYQLDFTGKSNGFHSNSSGTNSSGSSNSWCIASSKASERD 380

Query: 368 LQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGV 427
           L+ ALDWACG GNVDCTAIQPSQPCF+PDTLVSHAS+ FNSYFQQN ATDVAC FGG GV
Sbjct: 381 LKGALDWACGPGNVDCTAIQPSQPCFQPDTLVSHASFVFNSYFQQNRATDVACSFGGAGV 440

Query: 428 EVNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTSR 465
           +VNKDPSYD C+Y T G NKT  ++N TA+  TSS+ST R
Sbjct: 441 KVNKDPSYDKCIYITAGGNKT-KATNATAL--TSSASTPR 477

BLAST of CmaCh10G006780 vs. TAIR 10
Match: AT4G29360.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 629.8 bits (1623), Expect = 1.8e-180
Identity = 306/487 (62.83%), Postives = 393/487 (80.70%), Query Frame = 0

Query: 11  SKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPNSD 70
           SK+G+CYGRNAD+LP+PN+V++L+Q  NIK++RIYD+NI VLKAFANTG+ELMIGVPN+D
Sbjct: 25  SKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNAD 84

Query: 71  LLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVLTGLK 130
           LL+FAQFQSNVD+WL N+ILPYYP++KIT I+VG EVTE+P+N + LV+PAM+N+ T LK
Sbjct: 85  LLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTALK 144

Query: 131 KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINIYPYY 190
           K+GL KKIK+SS+HSL +LSRSFPPS+ +FS  ++ FLKP+LEFL +N+SPFMI++YPYY
Sbjct: 145 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYY 204

Query: 191 AYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRVMVTE 250
           AYR+S   V L+YALFESSS+V+DP TGLLY+NMFDAQ+DA+YFAL A++F+T++VMVTE
Sbjct: 205 AYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTE 264

Query: 251 TGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNENRKPGL 310
           +GWPSKGSPKET+ATP+NA  YNTNLIRHVI + GTPA+PGE++DVY+FSLFNENRKPG+
Sbjct: 265 SGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPGI 324

Query: 311 DSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRT------------------- 370
           +SERNWG+FY + T+VY LDFTG+     S   ++T ++ +                   
Sbjct: 325 ESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGG 384

Query: 371 -----AWCIASSKASDMDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSY 430
                 WCIASS+AS  +LQ ALDWACG GNVDC+A+QP QPCFEPDT++SHASYAFN+Y
Sbjct: 385 GGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTY 444

Query: 431 FQQNGATDVACGFGGNGVEVNKDPSYDNCLY----ATIGKNKTIS---SSNTTAISSTSS 467
           +QQ+GA+ + C F G  VEV+KDPSY NCLY    AT G N+T++   + N TAI S  +
Sbjct: 445 YQQSGASSIDCSFNGASVEVDKDPSYGNCLYMIAPATDGFNRTMAGNITGNITAIDSPLA 504

BLAST of CmaCh10G006780 vs. TAIR 10
Match: AT4G29360.2 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 614.4 bits (1583), Expect = 7.9e-176
Identity = 293/463 (63.28%), Postives = 377/463 (81.43%), Query Frame = 0

Query: 11  SKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPNSD 70
           SK+G+CYGRNAD+LP+PN+V++L+Q  NIK++RIYD+NI VLKAFANTG+ELMIGVPN+D
Sbjct: 25  SKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDVLKAFANTGIELMIGVPNAD 84

Query: 71  LLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVLTGLK 130
           LL+FAQFQSNVD+WL N+ILPYYP++KIT I+VG EVTE+P+N + LV+PAM+N+ T LK
Sbjct: 85  LLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAPDNATGLVLPAMRNIHTALK 144

Query: 131 KAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINIYPYY 190
           K+GL KKIK+SS+HSL +LSRSFPPS+ +FS  ++ FLKP+LEFL +N+SPFMI++YPYY
Sbjct: 145 KSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKPMLEFLVENESPFMIDLYPYY 204

Query: 191 AYRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRVMVTE 250
           AYR+S   V L+YALFESSS+V+DP TGLLY+NMFDAQ+DA+YFAL A++F+T++VMVTE
Sbjct: 205 AYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLDAIYFALTAMSFKTVKVMVTE 264

Query: 251 TGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNENRKPGL 310
           +GWPSKGSPKET+ATP+NA  YNTNLIRHVI + GTPA+PGE++DVY+FSLFNENRKPG+
Sbjct: 265 SGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKPGEEIDVYLFSLFNENRKPGI 324

Query: 311 DSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRT------------------- 370
           +SERNWG+FY + T+VY LDFTG+     S   ++T ++ +                   
Sbjct: 325 ESERNWGMFYANGTNVYALDFTGENTTPVSPTNSTTGTSPSPSSSPIINGNSTVTIGGGG 384

Query: 371 -----AWCIASSKASDMDLQNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSY 430
                 WCIASS+AS  +LQ ALDWACG GNVDC+A+QP QPCFEPDT++SHASYAFN+Y
Sbjct: 385 GGGTKKWCIASSQASVTELQTALDWACGPGNVDCSAVQPDQPCFEPDTVLSHASYAFNTY 444

Query: 431 FQQNGATDVACGFGGNGVEVNKDPSYDNCLYATIGKNKTISSS 450
           +QQ+GA+ + C F G  VEV+KDPS    LY    +N +I S+
Sbjct: 445 YQQSGASSIDCSFNGASVEVDKDPS--KYLYTYTFQNTSIKSN 485

BLAST of CmaCh10G006780 vs. TAIR 10
Match: AT2G01630.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 395.2 bits (1014), Expect = 7.5e-110
Identity = 201/458 (43.89%), Postives = 291/458 (63.54%), Query Frame = 0

Query: 9   QGSKVGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPN 68
           Q S +GV  G    ++P+P +V  L++  NI  +R+YD++  +L AFA+TGV+++I VPN
Sbjct: 19  QDSLIGVNIGTEVTNMPSPTQVVALLKSQNINRVRLYDADRSMLLAFAHTGVQVIISVPN 78

Query: 69  SDLLSFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVLTG 128
             LL  +Q  +   +W+  ++  YYPA+ IT I VG+EV  S  N +S++V A+K +   
Sbjct: 79  DQLLGISQSNATAANWVTRNVAAYYPATNITTIAVGSEVLTSLTNAASVLVSALKYIQAA 138

Query: 129 LKKAGLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAFFLKPLLEFLADNQSPFMINIYP 188
           L  A L ++IKVS+ HS  ++  SFPPS   F+  +   + PLL+FL    SP ++N+YP
Sbjct: 139 LVTANLDRQIKVSTPHSSTIILDSFPPSQAFFNKTWDPVIVPLLKFLQSTGSPLLLNVYP 198

Query: 189 YYAYRESPSNVSLDYALF---ESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIR 248
           Y+ Y +S   + LDYALF   +++ E +D NT L YTN+FDA +DA YFA+  LNF  I 
Sbjct: 199 YFDYVQSNGVIPLDYALFQPLQANKEAVDANTLLHYTNVFDAIVDAAYFAMSYLNFTNIP 258

Query: 249 VMVTETGWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNEN 308
           ++VTE+GWPSKG P E  AT +NA TYN+NLI+HVIN TGTP  PG  +  YI+ L+NE+
Sbjct: 259 IVVTESGWPSKGGPSEHDATVENANTYNSNLIQHVINKTGTPKHPGTAVTTYIYELYNED 318

Query: 309 RKPGLDSERNWGLFYPDQTSVYNLDFTGKGAVDASTQANSTSSNRTAWCIASSKASDMDL 368
            +PG  SE+NWGLFY + T VY L   G GA+ A+   N T      +CIA  K     L
Sbjct: 319 TRPGPVSEKNWGLFYTNGTPVYTLRLAGAGAILANDTTNQT------FCIAKEKVDRKML 378

Query: 369 QNALDWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVE 428
           Q ALDWACG G VDC+A+   + C+EPD +V+H++YAFN+Y+Q+ G    +C F G    
Sbjct: 379 QAALDWACGPGKVDCSALMQGESCYEPDDVVAHSTYAFNAYYQKMGKASGSCDFKGVATV 438

Query: 429 VNKDPSYDNCLYATIGKNKTISSSNTTAISSTSSSSTS 464
              DPS   C++    K+     +NT+A++ +++S+TS
Sbjct: 439 TTTDPSRGTCVFPGSAKSNQTLGNNTSALAPSANSTTS 470

BLAST of CmaCh10G006780 vs. TAIR 10
Match: AT4G26830.1 (O-Glycosyl hydrolases family 17 protein )

HSP 1 Score: 375.2 bits (962), Expect = 8.1e-104
Identity = 193/430 (44.88%), Postives = 272/430 (63.26%), Query Frame = 0

Query: 13  VGVCYGRNADDLPTPNKVAQLVQLHNIKYLRIYDSNIQVLKAFANTGVELMIGVPNSDLL 72
           VGV YGR A++LP+P KV  L++   I  ++I+D++  VL A AN+ +++++ +PN  L 
Sbjct: 25  VGVNYGRIANNLPSPEKVVNLLKSQGINRIKIFDTDKNVLTALANSKIKVIVALPNELLS 84

Query: 73  SFAQFQSNVDSWLKNSILPYYPASKITYITVGAEVTESPNNVSSLVVPAMKNVLTGLKKA 132
           S A  QS  D+W+K  I+PY+PA++I  I VG EV   P  ++  +V AMKN+ T L K 
Sbjct: 85  SAASHQSFADNWIKTHIMPYFPATEIEAIAVGNEVFVDP-TITPYLVNAMKNIHTSLVKY 144

Query: 133 GLHKKIKVSSTHSLGVLSRSFPPSAGAFSSNYAF-FLKPLLEFLADNQSPFMINIYPYYA 192
            L K IK+SS  +L  L+ S+PPS+G+F        +KP+L  L    S  M+N YP++A
Sbjct: 145 KLDKAIKISSPIALSALANSYPPSSGSFKPELIEPVVKPMLALLQQTSSYLMVNAYPFFA 204

Query: 193 YRESPSNVSLDYALFESSSEVIDPNTGLLYTNMFDAQIDALYFALMALNFRTIRVMVTET 252
           Y  +   +SLDYALF+ ++  ID  TGL Y ++FDAQIDA+Y AL A+ F+ ++VMVTET
Sbjct: 205 YAANADKISLDYALFKENAGNIDSGTGLKYNSLFDAQIDAVYAALSAVGFKGVKVMVTET 264

Query: 253 GWPSKGSPKETSATPDNAQTYNTNLIRHVINNTGTPARPGEKLDVYIFSLFNENRKPGLD 312
           GWPS G   E  A+  NA  YN  L++ V+   GTP RP E L+VY+F+LFNEN+KPG  
Sbjct: 265 GWPSVGDENEIGASESNAAAYNAGLVKRVLTGKGTPLRPTEPLNVYLFALFNENQKPGPT 324

Query: 313 SERNWGLFYPDQTSVYNLDFTGKGA--VDASTQANSTSSNRTAWCIASSKASDMDLQNAL 372
           SERN+GLFYP++  VYN+ FT K    V+ +      +     WC+++ + +   LQ AL
Sbjct: 325 SERNYGLFYPNEGKVYNVPFTKKSTTPVNGNRGKVPVTHEGHTWCVSNGEVAKEKLQEAL 384

Query: 373 DWACGSGNVDCTAIQPSQPCFEPDTLVSHASYAFNSYFQQNGATDVACGFGGNGVEVNKD 432
           D+ACG G  DC  IQP   C+ P++L +HASYAFNSY+Q+N      C FGG    V + 
Sbjct: 385 DYACGEGGADCRPIQPGATCYHPESLEAHASYAFNSYYQKNSRRVGTCFFGGAAHVVTQP 444

Query: 433 PSYDNCLYAT 440
           P Y  C + T
Sbjct: 445 PRYGKCEFPT 453

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FJU96.5e-19974.13Glucan endo-1,3-beta-glucosidase 13 OS=Arabidopsis thaliana OX=3702 GN=At5g56590... [more]
Q8VYE52.6e-17962.83Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Q9ZU911.1e-10843.89Glucan endo-1,3-beta-glucosidase 3 OS=Arabidopsis thaliana OX=3702 GN=At2g01630 ... [more]
Q9C7U51.3e-10143.23Glucan endo-1,3-beta-glucosidase 2 OS=Arabidopsis thaliana OX=3702 GN=At1g66250 ... [more]
Q9M0691.2e-9940.88Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JF222.0e-27299.38(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111485174 PE=... [more]
A0A6J1H8Q88.9e-26897.50(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111461577 P... [more]
A0A0A0M1T02.5e-25492.08(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_1G575070 PE=3... [more]
A0A1S3BPG31.2e-25391.67(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103492273 PE=3 SV... [more]
A0A6J1CTD82.1e-25392.10(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111014572 ... [more]
Match NameE-valueIdentityDescription
XP_022987696.14.2e-27299.38glucan endo-1,3-beta-glucosidase 13-like [Cucurbita maxima][more]
XP_023516959.13.3e-26997.92glucan endo-1,3-beta-glucosidase 13-like [Cucurbita pepo subsp. pepo][more]
XP_022960916.11.8e-26797.50glucan endo-1,3-beta-glucosidase 13-like [Cucurbita moschata] >XP_022960917.1 gl... [more]
KAG7023623.12.4e-26797.29Glucan endo-1,3-beta-glucosidase 13 [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6589961.14.1e-26798.11Glucan endo-1,3-beta-glucosidase 13, partial [Cucurbita argyrosperma subsp. soro... [more]
Match NameE-valueIdentityDescription
AT5G56590.14.6e-20074.13O-Glycosyl hydrolases family 17 protein [more]
AT4G29360.11.8e-18062.83O-Glycosyl hydrolases family 17 protein [more]
AT4G29360.27.9e-17663.28O-Glycosyl hydrolases family 17 protein [more]
AT2G01630.17.5e-11043.89O-Glycosyl hydrolases family 17 protein [more]
AT4G26830.18.1e-10444.88O-Glycosyl hydrolases family 17 protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR012946X8 domainSMARTSM00768X8_clscoord: 352..437
e-value: 4.9E-45
score: 165.6
IPR012946X8 domainPFAMPF07983X8coord: 353..423
e-value: 1.9E-20
score: 73.3
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 13..331
e-value: 4.9E-98
score: 328.6
IPR000490Glycoside hydrolase family 17PROSITEPS00587GLYCOSYL_HYDROL_F17coord: 244..257
NoneNo IPR availableGENE3D1.20.58.1040coord: 345..465
e-value: 1.9E-10
score: 43.0
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 11..338
e-value: 6.7E-128
score: 428.1
NoneNo IPR availablePANTHERPTHR32227:SF88GLUCAN ENDO-1,3-BETA-GLUCOSIDASE 13coord: 6..451
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 6..451
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 13..331

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G006780.1CmaCh10G006780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds