CmaCh09G004610 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh09G004610
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionethylene-responsive transcription factor SHINE 2-like
LocationCma_Chr09: 2028621 .. 2030524 (+)
RNA-Seq ExpressionCmaCh09G004610
SyntenyCmaCh09G004610
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCGAAACAGGCTCTTATTCAATTCAAACACTCCATCTTCCTCACTGGGATCATAAATGGCGCTTTATGATCTAAACCTGCAAATTTTCCTCTTCCCTTTCCCCATTCCCACAAAGCTCAGAAGTAAAATTTAATGAAACCTTCTTTTTGGATAGCAGACAGATTAGAAGTTTTGGGGGTTCCTTTTCGATCTGCCATTGGAGAGAGAGAAGAAAAGTTGCAGAAATGGGAGAGAGATTTGAAATGAGCGGAATCTCATTAAATTTTGGTTGTTAAGAAGCTGCGACTGTGGATCTTTCATGGTGAGCAGGTGAAAGTTTTTAATGGCCAACCCCATTTAAAAAGTACTATTGTGTTGTTGTTGTTGTTCTTCTTCTTCTCTATCAAATAATTTTCAATTTCCACCGACAATTCTATATAAATGGCTGAAATTATTACTCTCTGTACGTTTTTCAACTCTCTCACCTGTTTTTTTTTGTTTTTTTTTTTTTTTTAATTTCTCTATCTCTCTCTTGTTGAAGGCTCTGAAATTCTCTGTGTTTCTCTCTCTAGAGTTATGGTTTATTCAAGGAAGTTCAGAGGAGTCAGGCAGCGGCAGTGGGGCTCTTGGGTGTCAGAAATCCGCCACCCGTTGCTGTAAATTCACTCTTACATTACGTTGGTTGTTCTTTTGCTATAAATATATGCGCCATTGGTTGGGTTTATTGGTTCGATTGTCCTCTGTTTCCTCCATTTTCTGTCTGTTGCCCATAACCTGTTTGATGATTTGCCTGAAAGAAACTGGTCTCCTTTGGTGGCATATGTTTTTGAAAACTCGTATACTGCATTATTGTCGTTATGTTTGTGATCAATTTATTGTGTTTATTGGTTAAAAATGCTGTGAAAACCCCCTTTTTTTTGGTGCCCATAAGGTGTTTGTTGATATGCCTGAAAGAATCTGGGTGGCCATGGCATGCATATTTCTGTAGATTTGTGAACTACAATTTCCTCTGCTGTAAATGTATGCATTGATTGTGTGTGTTGGTTCAGAAAAAAAGCTCTGAAAAATGGAAACCCACCATTGTTGTGCCGTAAACAAAGAAGCTAGCCGGCTATGGCCATTGACCACGCTCATTTCTGCTATACATTACACCTTGAGCCCACATTCTTATACGTTTCATGTGTTAATTTCTTTGTAGGAAGAGAAGGGTGTGGCTGGGGACATTCGACACAGCAGAGGATGCAGCACGAGCGTATGATCAAGCAGCCATCTTAATGAATGGCCAAAACGCCAAGACCAATTTCCCTGCATCAAAAGACCAATCTGCAGAAGCCAGCCATGGCCATGGGCACGGATCTTCTCCAATGACCCCAAAAGCACTCTCAGAGCTACTCAGCACAAAGCTCAGAAAGTGCTGCAAGAACCCATCTCCTTCCTTGACATGCCTGCGGCTCGACAGTGACAACGCCCACATCGGTGTCTGGCAGAAGCGCGCAGGCACACGAGCCACTTCCAATTGGGTCATGAGGATTGAGCTAGGGAAGAAGGAGATCCCGTCCCCATTGCCATCGCCCGAATCGACGAGCCAGGAAGCCGATGGCCATCGGAACGGAATCGATGAAGAAGACAGAATTGCAATGCAAATGATTGAAGAGTTACTCAATTGGAACTGCCCTCTTCCTTCAATTTCAAGCTAAGTACAAGATCTCAACTCTAAAAATAAAGTAACAATAGTAATAAAACCCCATAAGATCAAAACAAATAGGCTAATTATGTGTATATATATATATATATATATCTGTTGTGCTTACTGTTTATGGGACTTTGGGAAGCTCAAAATCTGTATAAAGGGAGAGCTTTTATAAATATTGTCTGTTTTTTTTTTTGTCGATTTTAATCATATATATATATATATAGAAATGAAT

mRNA sequence

GCGAAACAGGCTCTTATTCAATTCAAACACTCCATCTTCCTCACTGGGATCATAAATGGCGCTTTATGATCTAAACCTGCAAATTTTCCTCTTCCCTTTCCCCATTCCCACAAAGCTCAGAAGTAAAATTTAATGAAACCTTCTTTTTGGATAGCAGACAGATTAGAAGTTTTGGGGGTTCCTTTTCGATCTGCCATTGGAGAGAGAGAAGAAAAGTTGCAGAAATGGGAGAGAGATTTGAAATGAGCGGAATCTCATTAAATTTTGGTTGTTAAGAAGCTGCGACTGTGGATCTTTCATGGTGAGCAGAGTTATGGTTTATTCAAGGAAGTTCAGAGGAGTCAGGCAGCGGCAGTGGGGCTCTTGGGTGTCAGAAATCCGCCACCCGTTGCTGAAGAGAAGGGTGTGGCTGGGGACATTCGACACAGCAGAGGATGCAGCACGAGCGTATGATCAAGCAGCCATCTTAATGAATGGCCAAAACGCCAAGACCAATTTCCCTGCATCAAAAGACCAATCTGCAGAAGCCAGCCATGGCCATGGGCACGGATCTTCTCCAATGACCCCAAAAGCACTCTCAGAGCTACTCAGCACAAAGCTCAGAAAGTGCTGCAAGAACCCATCTCCTTCCTTGACATGCCTGCGGCTCGACAGTGACAACGCCCACATCGGTGTCTGGCAGAAGCGCGCAGGCACACGAGCCACTTCCAATTGGGTCATGAGGATTGAGCTAGGGAAGAAGGAGATCCCGTCCCCATTGCCATCGCCCGAATCGACGAGCCAGGAAGCCGATGGCCATCGGAACGGAATCGATGAAGAAGACAGAATTGCAATGCAAATGATTGAAGAGTTACTCAATTGGAACTGCCCTCTTCCTTCAATTTCAAGCTAAGTACAAGATCTCAACTCTAAAAATAAAGTAACAATAGTAATAAAACCCCATAAGATCAAAACAAATAGGCTAATTATGTGTATATATATATATATATATATCTGTTGTGCTTACTGTTTATGGGACTTTGGGAAGCTCAAAATCTGTATAAAGGGAGAGCTTTTATAAATATTGTCTGTTTTTTTTTTTGTCGATTTTAATCATATATATATATATATAGAAATGAAT

Coding sequence (CDS)

ATGGTGAGCAGAGTTATGGTTTATTCAAGGAAGTTCAGAGGAGTCAGGCAGCGGCAGTGGGGCTCTTGGGTGTCAGAAATCCGCCACCCGTTGCTGAAGAGAAGGGTGTGGCTGGGGACATTCGACACAGCAGAGGATGCAGCACGAGCGTATGATCAAGCAGCCATCTTAATGAATGGCCAAAACGCCAAGACCAATTTCCCTGCATCAAAAGACCAATCTGCAGAAGCCAGCCATGGCCATGGGCACGGATCTTCTCCAATGACCCCAAAAGCACTCTCAGAGCTACTCAGCACAAAGCTCAGAAAGTGCTGCAAGAACCCATCTCCTTCCTTGACATGCCTGCGGCTCGACAGTGACAACGCCCACATCGGTGTCTGGCAGAAGCGCGCAGGCACACGAGCCACTTCCAATTGGGTCATGAGGATTGAGCTAGGGAAGAAGGAGATCCCGTCCCCATTGCCATCGCCCGAATCGACGAGCCAGGAAGCCGATGGCCATCGGAACGGAATCGATGAAGAAGACAGAATTGCAATGCAAATGATTGAAGAGTTACTCAATTGGAACTGCCCTCTTCCTTCAATTTCAAGCTAA

Protein sequence

MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEELLNWNCPLPSISS
Homology
BLAST of CmaCh09G004610 vs. ExPASy Swiss-Prot
Match: Q3E958 (Ethylene-responsive transcription factor SHINE 3 OS=Arabidopsis thaliana OX=3702 GN=SHN3 PE=2 SV=1)

HSP 1 Score: 233.0 bits (593), Expect = 2.8e-60
Identity = 117/186 (62.90%), Postives = 142/186 (76.34%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MV+S+KFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAE AARAYDQAA+LMNGQ+AKT
Sbjct: 1   MVHSKKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAETAARAYDQAAVLMNGQSAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFP  K   +  S+     S+  +PK+LSELL+ KLRK CK+ +P LTCLRLD+D++HIG
Sbjct: 61  NFPVIK---SNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 185
           VWQKRAG++ + NWV  +ELG K    P    E+   +       + E+D++AMQMIEEL
Sbjct: 121 VWQKRAGSKTSPNWVKLVELGDKVNARPGGDIETNKMKV--RNEDVQEDDQMAMQMIEEL 180

Query: 186 LNWNCP 192
           LNW CP
Sbjct: 181 LNWTCP 181

BLAST of CmaCh09G004610 vs. ExPASy Swiss-Prot
Match: Q9LFN7 (Ethylene-responsive transcription factor SHINE 2 OS=Arabidopsis thaliana OX=3702 GN=SHN2 PE=2 SV=1)

HSP 1 Score: 232.6 bits (592), Expect = 3.7e-60
Identity = 118/190 (62.11%), Postives = 146/190 (76.84%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MV+SRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTF+TAE AARAYDQAA+LMNGQNAKT
Sbjct: 1   MVHSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFETAEAAARAYDQAALLMNGQNAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFP  K +   + H     S  M+PK+LSELL+ KLRK CK+ +PSLTCLRLD+D++HIG
Sbjct: 61  NFPVVKSEEG-SDHVKDVNSPLMSPKSLSELLNAKLRKSCKDLTPSLTCLRLDTDSSHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLP------SPESTSQEADGHRNGIDEEDRIAM 185
           VWQKRAG++ +  WVMR+ELG     S +       + ++  +E +     I +ED++AM
Sbjct: 121 VWQKRAGSKTSPTWVMRLELGNVVNESAVDLGLTTMNKQNVEKEEEEEEAIISDEDQLAM 180

Query: 186 QMIEELLNWN 190
           +MIEELLNW+
Sbjct: 181 EMIEELLNWS 189

BLAST of CmaCh09G004610 vs. ExPASy Swiss-Prot
Match: Q9XI33 (Ethylene-responsive transcription factor WIN1 OS=Arabidopsis thaliana OX=3702 GN=WIN1 PE=2 SV=1)

HSP 1 Score: 222.2 bits (565), Expect = 5.0e-57
Identity = 114/199 (57.29%), Postives = 144/199 (72.36%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MV ++KFRGVRQR WGSWV+EIRHPLLKRR+WLGTF+TAE+AARAYD+AA+LM+G+NAKT
Sbjct: 1   MVQTKKFRGVRQRHWGSWVAEIRHPLLKRRIWLGTFETAEEAARAYDEAAVLMSGRNAKT 60

Query: 66  NFPASKDQSAEASHG------HGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDS 125
           NFP + + + E S G          SS  +  +LS +LS KLRKCCK+PSPSLTCLRLD+
Sbjct: 61  NFPLNNNNTGETSEGKTDISASSTMSSSTSSSSLSSILSAKLRKCCKSPSPSLTCLRLDT 120

Query: 126 DNAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPES--------TSQEADGHRNGI 185
            ++HIGVWQKRAG+++ S+WVM +ELG         S  S        T  E  G R  +
Sbjct: 121 ASSHIGVWQKRAGSKSDSSWVMTVELGPASSSQETTSKASQDAILAPTTEVEIGGSREEV 180

Query: 186 -DEEDRIAMQMIEELLNWN 190
            DEE+++A+QMIEELLN N
Sbjct: 181 LDEEEKVALQMIEELLNTN 199

BLAST of CmaCh09G004610 vs. ExPASy Swiss-Prot
Match: Q94AW5 (Ethylene-responsive transcription factor ERF003 OS=Arabidopsis thaliana OX=3702 GN=ERF003 PE=1 SV=1)

HSP 1 Score: 108.2 bits (269), Expect = 1.1e-22
Identity = 56/95 (58.95%), Postives = 66/95 (69.47%), Query Frame = 0

Query: 10  RKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKTNFPA 69
           ++FRGVRQR WGSWVSEIRHPLLK R+WLGTF+TAEDAARAYD+AA LM G  A+TNFP 
Sbjct: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65

Query: 70  SKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKC 105
           + +    +S            K LS  L+ KL KC
Sbjct: 66  NPNAIPTSS-----------SKLLSATLTAKLHKC 89

BLAST of CmaCh09G004610 vs. ExPASy Swiss-Prot
Match: P16146 (Protein PPLZ02 OS=Lupinus polyphyllus OX=3874 GN=PPLZ02 PE=2 SV=1)

HSP 1 Score: 97.8 bits (242), Expect = 1.4e-19
Identity = 50/95 (52.63%), Postives = 63/95 (66.32%), Query Frame = 0

Query: 10  RKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKTNFPA 69
           +++RG RQR WGSWVSEIRH +LK R+W GTF++AEDAARAYD+AA LM G  A+TNFP 
Sbjct: 6   QRYRGFRQRHWGSWVSEIRHSILKTRIWQGTFESAEDAARAYDEAARLMCGTRARTNFPY 65

Query: 70  SKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKC 105
           + + S  +S            K LS  L  KL +C
Sbjct: 66  NPNASQSSS-----------SKLLSATLIAKLHRC 89

BLAST of CmaCh09G004610 vs. ExPASy TrEMBL
Match: A0A6J1IGA2 (ethylene-responsive transcription factor SHINE 2 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111476664 PE=4 SV=1)

HSP 1 Score: 404.4 bits (1038), Expect = 2.6e-109
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0

Query: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60
           MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG
Sbjct: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60

Query: 61  QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120
           QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD
Sbjct: 61  QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120

Query: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ 180
           NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ
Sbjct: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ 180

Query: 181 MIEELLNWNCPLPSISS 198
           MIEELLNWNCPLPSISS
Sbjct: 181 MIEELLNWNCPLPSISS 197

BLAST of CmaCh09G004610 vs. ExPASy TrEMBL
Match: A0A6J1IIU8 (ethylene-responsive transcription factor SHINE 2 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111476664 PE=4 SV=1)

HSP 1 Score: 396.0 bits (1016), Expect = 9.4e-107
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT
Sbjct: 1   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG
Sbjct: 61  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 185
           VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL
Sbjct: 121 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 180

Query: 186 LNWNCPLPSISS 198
           LNWNCPLPSISS
Sbjct: 181 LNWNCPLPSISS 192

BLAST of CmaCh09G004610 vs. ExPASy TrEMBL
Match: A0A6J1F8L5 (ethylene-responsive transcription factor SHINE 2 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111443124 PE=4 SV=1)

HSP 1 Score: 392.5 bits (1007), Expect = 1.0e-105
Identity = 194/197 (98.48%), Postives = 194/197 (98.48%), Query Frame = 0

Query: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60
           MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG
Sbjct: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60

Query: 61  QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120
           QNAKTNFPASKDQSAEAS  HGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD
Sbjct: 61  QNAKTNFPASKDQSAEAS--HGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120

Query: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ 180
           NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQE DGHRNGIDEEDRIAMQ
Sbjct: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEPDGHRNGIDEEDRIAMQ 180

Query: 181 MIEELLNWNCPLPSISS 198
           MIEELLNWNCPLPSISS
Sbjct: 181 MIEELLNWNCPLPSISS 195

BLAST of CmaCh09G004610 vs. ExPASy TrEMBL
Match: A0A6J1FE03 (ethylene-responsive transcription factor SHINE 2 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111443124 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 3.7e-103
Identity = 189/192 (98.44%), Postives = 189/192 (98.44%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT
Sbjct: 1   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFPASKDQSAEAS  HGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG
Sbjct: 61  NFPASKDQSAEAS--HGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 185
           VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQE DGHRNGIDEEDRIAMQMIEEL
Sbjct: 121 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEPDGHRNGIDEEDRIAMQMIEEL 180

Query: 186 LNWNCPLPSISS 198
           LNWNCPLPSISS
Sbjct: 181 LNWNCPLPSISS 190

BLAST of CmaCh09G004610 vs. ExPASy TrEMBL
Match: A0A6J1BYX9 (ethylene-responsive transcription factor SHINE 2-like OS=Momordica charantia OX=3673 GN=LOC111006480 PE=4 SV=1)

HSP 1 Score: 351.7 bits (901), Expect = 2.0e-93
Identity = 177/200 (88.50%), Postives = 182/200 (91.00%), Query Frame = 0

Query: 2   VSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQ 61
           +S VMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQ
Sbjct: 78  LSIVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQ 137

Query: 62  NAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDN 121
           NAKTNFPASK+Q+ E    HG GSSP++PKALSELLSTKLRKCCKNPSPSLTCLRLDSDN
Sbjct: 138 NAKTNFPASKNQAEEGR--HGDGSSPLSPKALSELLSTKLRKCCKNPSPSLTCLRLDSDN 197

Query: 122 AHIGVWQKRAGTRATSNWVMRIELGKK----EIPSPLPSPESTSQEADGHRNGIDEEDRI 181
           AHIGVWQKRAGTRA SNWVMRIELGKK    E PSP PS ES S EADGHRNGIDEEDRI
Sbjct: 198 AHIGVWQKRAGTRAASNWVMRIELGKKETETETPSPSPSLESASGEADGHRNGIDEEDRI 257

Query: 182 AMQMIEELLNWNCPLPSISS 198
           AMQMIEELLNWNCPLPS SS
Sbjct: 258 AMQMIEELLNWNCPLPSTSS 275

BLAST of CmaCh09G004610 vs. NCBI nr
Match: XP_022976201.1 (ethylene-responsive transcription factor SHINE 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 404.4 bits (1038), Expect = 5.4e-109
Identity = 197/197 (100.00%), Postives = 197/197 (100.00%), Query Frame = 0

Query: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60
           MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG
Sbjct: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60

Query: 61  QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120
           QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD
Sbjct: 61  QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120

Query: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ 180
           NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ
Sbjct: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ 180

Query: 181 MIEELLNWNCPLPSISS 198
           MIEELLNWNCPLPSISS
Sbjct: 181 MIEELLNWNCPLPSISS 197

BLAST of CmaCh09G004610 vs. NCBI nr
Match: XP_023536493.1 (ethylene-responsive transcription factor SHINE 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 398.7 bits (1023), Expect = 3.0e-107
Identity = 194/197 (98.48%), Postives = 194/197 (98.48%), Query Frame = 0

Query: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60
           MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG
Sbjct: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60

Query: 61  QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120
           QNAKTNFPASKDQS EASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD
Sbjct: 61  QNAKTNFPASKDQSTEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120

Query: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ 180
           NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQE D HRNGIDEEDRIAMQ
Sbjct: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEPDAHRNGIDEEDRIAMQ 180

Query: 181 MIEELLNWNCPLPSISS 198
           MIEELLNWNCPLPSISS
Sbjct: 181 MIEELLNWNCPLPSISS 197

BLAST of CmaCh09G004610 vs. NCBI nr
Match: XP_022976202.1 (ethylene-responsive transcription factor SHINE 2 isoform X2 [Cucurbita maxima] >XP_022976203.1 ethylene-responsive transcription factor SHINE 2 isoform X2 [Cucurbita maxima] >XP_022976204.1 ethylene-responsive transcription factor SHINE 2 isoform X2 [Cucurbita maxima] >XP_022976205.1 ethylene-responsive transcription factor SHINE 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 396.0 bits (1016), Expect = 1.9e-106
Identity = 192/192 (100.00%), Postives = 192/192 (100.00%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT
Sbjct: 1   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG
Sbjct: 61  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 185
           VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL
Sbjct: 121 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 180

Query: 186 LNWNCPLPSISS 198
           LNWNCPLPSISS
Sbjct: 181 LNWNCPLPSISS 192

BLAST of CmaCh09G004610 vs. NCBI nr
Match: KAG7024462.1 (Ethylene-responsive transcription factor SHINE 3, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 392.9 bits (1008), Expect = 1.6e-105
Identity = 190/192 (98.96%), Postives = 191/192 (99.48%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT
Sbjct: 1   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG
Sbjct: 61  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 185
           VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTS+E DGHRNGIDEEDRIAMQMIEEL
Sbjct: 121 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSEEPDGHRNGIDEEDRIAMQMIEEL 180

Query: 186 LNWNCPLPSISS 198
           LNWNCPLPSISS
Sbjct: 181 LNWNCPLPSISS 192

BLAST of CmaCh09G004610 vs. NCBI nr
Match: XP_022936544.1 (ethylene-responsive transcription factor SHINE 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 392.5 bits (1007), Expect = 2.1e-105
Identity = 194/197 (98.48%), Postives = 194/197 (98.48%), Query Frame = 0

Query: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60
           MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG
Sbjct: 1   MVSRVMVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNG 60

Query: 61  QNAKTNFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120
           QNAKTNFPASKDQSAEAS  HGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD
Sbjct: 61  QNAKTNFPASKDQSAEAS--HGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSD 120

Query: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQ 180
           NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQE DGHRNGIDEEDRIAMQ
Sbjct: 121 NAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEPDGHRNGIDEEDRIAMQ 180

Query: 181 MIEELLNWNCPLPSISS 198
           MIEELLNWNCPLPSISS
Sbjct: 181 MIEELLNWNCPLPSISS 195

BLAST of CmaCh09G004610 vs. TAIR 10
Match: AT5G25390.2 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 233.0 bits (593), Expect = 2.0e-61
Identity = 117/186 (62.90%), Postives = 142/186 (76.34%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MV+S+KFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAE AARAYDQAA+LMNGQ+AKT
Sbjct: 1   MVHSKKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAETAARAYDQAAVLMNGQSAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFP  K   +  S+     S+  +PK+LSELL+ KLRK CK+ +P LTCLRLD+D++HIG
Sbjct: 61  NFPVIK---SNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 185
           VWQKRAG++ + NWV  +ELG K    P    E+   +       + E+D++AMQMIEEL
Sbjct: 121 VWQKRAGSKTSPNWVKLVELGDKVNARPGGDIETNKMKV--RNEDVQEDDQMAMQMIEEL 180

Query: 186 LNWNCP 192
           LNW CP
Sbjct: 181 LNWTCP 181

BLAST of CmaCh09G004610 vs. TAIR 10
Match: AT5G11190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 232.6 bits (592), Expect = 2.6e-61
Identity = 118/190 (62.11%), Postives = 146/190 (76.84%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MV+SRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTF+TAE AARAYDQAA+LMNGQNAKT
Sbjct: 1   MVHSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFETAEAAARAYDQAALLMNGQNAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFP  K +   + H     S  M+PK+LSELL+ KLRK CK+ +PSLTCLRLD+D++HIG
Sbjct: 61  NFPVVKSEEG-SDHVKDVNSPLMSPKSLSELLNAKLRKSCKDLTPSLTCLRLDTDSSHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLP------SPESTSQEADGHRNGIDEEDRIAM 185
           VWQKRAG++ +  WVMR+ELG     S +       + ++  +E +     I +ED++AM
Sbjct: 121 VWQKRAGSKTSPTWVMRLELGNVVNESAVDLGLTTMNKQNVEKEEEEEEAIISDEDQLAM 180

Query: 186 QMIEELLNWN 190
           +MIEELLNW+
Sbjct: 181 EMIEELLNWS 189

BLAST of CmaCh09G004610 vs. TAIR 10
Match: AT1G15360.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 3.6e-58
Identity = 114/199 (57.29%), Postives = 144/199 (72.36%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MV ++KFRGVRQR WGSWV+EIRHPLLKRR+WLGTF+TAE+AARAYD+AA+LM+G+NAKT
Sbjct: 1   MVQTKKFRGVRQRHWGSWVAEIRHPLLKRRIWLGTFETAEEAARAYDEAAVLMSGRNAKT 60

Query: 66  NFPASKDQSAEASHG------HGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDS 125
           NFP + + + E S G          SS  +  +LS +LS KLRKCCK+PSPSLTCLRLD+
Sbjct: 61  NFPLNNNNTGETSEGKTDISASSTMSSSTSSSSLSSILSAKLRKCCKSPSPSLTCLRLDT 120

Query: 126 DNAHIGVWQKRAGTRATSNWVMRIELGKKEIPSPLPSPES--------TSQEADGHRNGI 185
            ++HIGVWQKRAG+++ S+WVM +ELG         S  S        T  E  G R  +
Sbjct: 121 ASSHIGVWQKRAGSKSDSSWVMTVELGPASSSQETTSKASQDAILAPTTEVEIGGSREEV 180

Query: 186 -DEEDRIAMQMIEELLNWN 190
            DEE+++A+QMIEELLN N
Sbjct: 181 LDEEEKVALQMIEELLNTN 199

BLAST of CmaCh09G004610 vs. TAIR 10
Match: AT5G25390.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 222.2 bits (565), Expect = 3.6e-58
Identity = 114/186 (61.29%), Postives = 139/186 (74.73%), Query Frame = 0

Query: 6   MVYSRKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKT 65
           MV+S+KFRGVRQRQWGSWVSEIRHPLL   VWLGTFDTAE AARAYDQAA+LMNGQ+AKT
Sbjct: 1   MVHSKKFRGVRQRQWGSWVSEIRHPLL---VWLGTFDTAETAARAYDQAAVLMNGQSAKT 60

Query: 66  NFPASKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKCCKNPSPSLTCLRLDSDNAHIG 125
           NFP  K   +  S+     S+  +PK+LSELL+ KLRK CK+ +P LTCLRLD+D++HIG
Sbjct: 61  NFPVIK---SNGSNSLEINSALRSPKSLSELLNAKLRKNCKDQTPYLTCLRLDNDSSHIG 120

Query: 126 VWQKRAGTRATSNWVMRIELGKKEIPSPLPSPESTSQEADGHRNGIDEEDRIAMQMIEEL 185
           VWQKRAG++ + NWV  +ELG K    P    E+   +       + E+D++AMQMIEEL
Sbjct: 121 VWQKRAGSKTSPNWVKLVELGDKVNARPGGDIETNKMKV--RNEDVQEDDQMAMQMIEEL 178

Query: 186 LNWNCP 192
           LNW CP
Sbjct: 181 LNWTCP 178

BLAST of CmaCh09G004610 vs. TAIR 10
Match: AT5G25190.1 (Integrase-type DNA-binding superfamily protein )

HSP 1 Score: 108.2 bits (269), Expect = 7.5e-24
Identity = 56/95 (58.95%), Postives = 66/95 (69.47%), Query Frame = 0

Query: 10  RKFRGVRQRQWGSWVSEIRHPLLKRRVWLGTFDTAEDAARAYDQAAILMNGQNAKTNFPA 69
           ++FRGVRQR WGSWVSEIRHPLLK R+WLGTF+TAEDAARAYD+AA LM G  A+TNFP 
Sbjct: 6   QRFRGVRQRHWGSWVSEIRHPLLKTRIWLGTFETAEDAARAYDEAARLMCGPRARTNFPY 65

Query: 70  SKDQSAEASHGHGHGSSPMTPKALSELLSTKLRKC 105
           + +    +S            K LS  L+ KL KC
Sbjct: 66  NPNAIPTSS-----------SKLLSATLTAKLHKC 89

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q3E9582.8e-6062.90Ethylene-responsive transcription factor SHINE 3 OS=Arabidopsis thaliana OX=3702... [more]
Q9LFN73.7e-6062.11Ethylene-responsive transcription factor SHINE 2 OS=Arabidopsis thaliana OX=3702... [more]
Q9XI335.0e-5757.29Ethylene-responsive transcription factor WIN1 OS=Arabidopsis thaliana OX=3702 GN... [more]
Q94AW51.1e-2258.95Ethylene-responsive transcription factor ERF003 OS=Arabidopsis thaliana OX=3702 ... [more]
P161461.4e-1952.63Protein PPLZ02 OS=Lupinus polyphyllus OX=3874 GN=PPLZ02 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1IGA22.6e-109100.00ethylene-responsive transcription factor SHINE 2 isoform X1 OS=Cucurbita maxima ... [more]
A0A6J1IIU89.4e-107100.00ethylene-responsive transcription factor SHINE 2 isoform X2 OS=Cucurbita maxima ... [more]
A0A6J1F8L51.0e-10598.48ethylene-responsive transcription factor SHINE 2 isoform X1 OS=Cucurbita moschat... [more]
A0A6J1FE033.7e-10398.44ethylene-responsive transcription factor SHINE 2 isoform X2 OS=Cucurbita moschat... [more]
A0A6J1BYX92.0e-9388.50ethylene-responsive transcription factor SHINE 2-like OS=Momordica charantia OX=... [more]
Match NameE-valueIdentityDescription
XP_022976201.15.4e-109100.00ethylene-responsive transcription factor SHINE 2 isoform X1 [Cucurbita maxima][more]
XP_023536493.13.0e-10798.48ethylene-responsive transcription factor SHINE 2 isoform X1 [Cucurbita pepo subs... [more]
XP_022976202.11.9e-106100.00ethylene-responsive transcription factor SHINE 2 isoform X2 [Cucurbita maxima] >... [more]
KAG7024462.11.6e-10598.96Ethylene-responsive transcription factor SHINE 3, partial [Cucurbita argyrosperm... [more]
XP_022936544.12.1e-10598.48ethylene-responsive transcription factor SHINE 2 isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
AT5G25390.22.0e-6162.90Integrase-type DNA-binding superfamily protein [more]
AT5G11190.12.6e-6162.11Integrase-type DNA-binding superfamily protein [more]
AT1G15360.13.6e-5857.29Integrase-type DNA-binding superfamily protein [more]
AT5G25390.13.6e-5861.29Integrase-type DNA-binding superfamily protein [more]
AT5G25190.17.5e-2458.95Integrase-type DNA-binding superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001471AP2/ERF domainPRINTSPR00367ETHRSPELEMNTcoord: 12..23
score: 60.91
coord: 34..50
score: 72.19
IPR001471AP2/ERF domainSMARTSM00380rav1_2coord: 11..74
e-value: 7.4E-40
score: 148.4
IPR001471AP2/ERF domainPFAMPF00847AP2coord: 11..60
e-value: 4.0E-13
score: 49.4
IPR001471AP2/ERF domainPROSITEPS51032AP2_ERFcoord: 11..68
score: 21.904358
IPR001471AP2/ERF domainCDDcd00018AP2coord: 11..70
e-value: 2.28162E-34
score: 114.291
IPR036955AP2/ERF domain superfamilyGENE3D3.30.730.10AP2/ERF domaincoord: 10..70
e-value: 5.1E-27
score: 95.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 64..91
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 148..168
NoneNo IPR availablePANTHERPTHR31194:SF13ETHYLENE-RESPONSIVE TRANSCRIPTION FACTOR SHINE 2coord: 6..195
NoneNo IPR availablePANTHERPTHR31194SHN SHINE , DNA BINDING / TRANSCRIPTION FACTORcoord: 6..195
IPR016177DNA-binding domain superfamilySUPERFAMILY54171DNA-binding domaincoord: 11..70

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh09G004610.1CmaCh09G004610.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding
molecular_function GO:0003700 DNA-binding transcription factor activity