CmaCh06G007780 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh06G007780
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: (RGF1), (RGF2), (RGF3), (RGF4), (RGF5), (RGF6), (RGF7), (RGF8) and (RGF9).
Location	Cma_Chr06: 4021984 .. 4022943 (-)
RNA-Seq Expression	CmaCh06G007780
Synteny	CmaCh06G007780

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TATTTCTCTTTCCATTTCCATGCCCATGCCAACTTCTTTGTTTCTCGCTCTCCTATTCTTGTCAACTTTTTTGCATGCATGCGATGCTCGTCTTCTTAAATTATGCCACAGCTCGAGGCTCGAATCGTGTATGATCTCTCCAGGGTTTCGAATGGATCAACTCAAGAGAAGTTTGGATAAAAGCAAAAGTAACGAGGTTTCTTCCGTGGAGAATGTTGGGATTGAAATATTGAAGGTATATCAACTTGTTTAGCTCTTAACCCTGATAATATTCGTCTTTCTCATGGAGGCTCATGGGTCTAACTAACCCCGAGCTGATAGTAGTCTCTTTTACCTATTTGCTACTATAAGAACCTAAAAATAATGATCAAGAACTTGTTCGACTTTAAAATAATCAAATCATTCCAATGTGAAAATTATATAATAGAATCGTTTTCATGGTTTTTGGTCGAGATATTCATCGACATATTATCATTTTTTTTATTATTTTTGGTTTACACGTGAAATCCAATCGTTTCTATCAACATTTCCTTCGACAACAACATTTAATTTTTAGGTCAAGACAAGGCATTAATATAGACAAAAGCATATGAATGATAAATGCATGGAAAAGAGATATTAAAATAACAATCACCATTAATGGACCTTGGAAATTTAGCAGGGCATGAAGAAGCTTAGAAGGTTGGCAATGGAGCTTCCAGTGAGAGACAGAGACAGAGACAAAGACAAAGACATAGACAAAGTGGTGAACTCCGAGGAAAACGAGGTTGGAGAAGACATGGTCGTGATGGATTATGCACAGCCCCACAGGAAACCTCCTATTCACAATCGAAAACCCTAGCTCACAACTTCATATCTCTTCGGAATTTCCTATGATCTTCAAAATATGTTCCAAAAATCAATGTATTGTATCTAAGGGTCTACTATATTCAAGATTTTTGTGTTAAATAAAAGCATGCA

mRNA sequence

TATTTCTCTTTCCATTTCCATGCCCATGCCAACTTCTTTGTTTCTCGCTCTCCTATTCTTGTCAACTTTTTTGCATGCATGCGATGCTCGTCTTCTTAAATTATGCCACAGCTCGAGGCTCGAATCGTGTATGATCTCTCCAGGGTTTCGAATGGATCAACTCAAGAGAAGTTTGGATAAAAGCAAAAGTAACGAGGTTTCTTCCGTGGAGAATGTTGGGATTGAAATATTGAAGGGCATGAAGAAGCTTAGAAGGTTGGCAATGGAGCTTCCAGTGAGAGACAGAGACAGAGACAAAGACAAAGACATAGACAAAGTGGTGAACTCCGAGGAAAACGAGGTTGGAGAAGACATGGTCGTGATGGATTATGCACAGCCCCACAGGAAACCTCCTATTCACAATCGAAAACCCTAGCTCACAACTTCATATCTCTTCGGAATTTCCTATGATCTTCAAAATATGTTCCAAAAATCAATGTATTGTATCTAAGGGTCTACTATATTCAAGATTTTTGTGTTAAATAAAAGCATGCA

Coding sequence (CDS)

ATGCCCATGCCAACTTCTTTGTTTCTCGCTCTCCTATTCTTGTCAACTTTTTTGCATGCATGCGATGCTCGTCTTCTTAAATTATGCCACAGCTCGAGGCTCGAATCGTGTATGATCTCTCCAGGGTTTCGAATGGATCAACTCAAGAGAAGTTTGGATAAAAGCAAAAGTAACGAGGTTTCTTCCGTGGAGAATGTTGGGATTGAAATATTGAAGGGCATGAAGAAGCTTAGAAGGTTGGCAATGGAGCTTCCAGTGAGAGACAGAGACAGAGACAAAGACAAAGACATAGACAAAGTGGTGAACTCCGAGGAAAACGAGGTTGGAGAAGACATGGTCGTGATGGATTATGCACAGCCCCACAGGAAACCTCCTATTCACAATCGAAAACCCTAG

Protein sequence

MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEVSSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQPHRKPPIHNRKP

Homology

[Close]

BLAST of CmaCh06G007780 vs. ExPASy Swiss-Prot
Match: Q93VK8 (Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1)

HSP 1 Score: 46.2 bits (108), Expect = 3.3e-04
Identity = 19/24 (79.17%), Postives = 21/24 (87.50%), Query Frame = 0


Query: 107 EVGEDMVVMDYAQPHRKPPIHNRK 131
           E GE++VVMDY QPHRKPPIHN K
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

[Close]

BLAST of CmaCh06G007780 vs. ExPASy TrEMBL
Match: A0A6J1KZM9 (uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521 PE=4 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 3.8e-64
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV 60
           MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV
Sbjct: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV 60

Query: 61  SSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP 120
           SSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP
Sbjct: 61  SSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP 120

Query: 121 HRKPPIHNRKP 132
           HRKPPIHNRKP
Sbjct: 121 HRKPPIHNRKP 131

[Close]

BLAST of CmaCh06G007780 vs. ExPASy TrEMBL
Match: A0A6J1DKL1 (uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021396 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 7.6e-20
Identity = 71/135 (52.59%), Postives = 90/135 (66.67%), Query Frame = 0


Query: 3   MPTSLFLALLFLSTFLHACDA-RLLKLCHSSRLESCM-----ISPGFRMDQLKRSLDKSK 62
           M T   L +L LST LHACDA   L+L   S   S +     I   FR DQ +RSL+K+ 
Sbjct: 1   MATPSLLFILLLST-LHACDACHRLQLFEYSNPVSRLGLLAPIPQEFRTDQPERSLNKNI 60

Query: 63  SNEVSSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMD 122
            ++VSS   +GI ILKGMKK +RLAME  ++++        +K+++ EENEVGEDMVVMD
Sbjct: 61  IHKVSSSVEIGIGILKGMKKHKRLAMEFSMKEK--------EKLMSYEENEVGEDMVVMD 120

Query: 123 YAQPHRKPPIHNRKP 132
           YAQPHRKPPIHN+KP
Sbjct: 121 YAQPHRKPPIHNKKP 126

[Close]

BLAST of CmaCh06G007780 vs. ExPASy TrEMBL
Match: A0A0A0L298 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 7.8e-17
Identity = 72/142 (50.70%), Postives = 89/142 (62.68%), Query Frame = 0


Query: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCM-----ISPG---FRMDQLKRSL 60
           M MP   FL LLF S+ LHACDA  L+   SS   S +     +S G      DQ + S 
Sbjct: 1   MAMP-YFFLILLFFSSTLHACDASRLRFFQSSNFVSRINELHSMSQGIWSINHDQ-EGSF 60

Query: 61  DKSKS-NEVSSVEN-VGIEILKGMKK-LRRLAMELPVRDRDRDKDKDIDKVVNSEENEVG 120
           DK+ + N + + EN +GIE+LKGMK+   RL ME P++          +KV+NS+ENEVG
Sbjct: 61  DKNNNDNILHNKENIIGIEMLKGMKRDGSRLVMEFPMK----------EKVMNSKENEVG 120

Query: 121 EDMVVMDYAQPHRKPPIHNRKP 132
           EDMVVMDYAQPHRKPPIHN KP
Sbjct: 121 EDMVVMDYAQPHRKPPIHNEKP 130

[Close]

BLAST of CmaCh06G007780 vs. ExPASy TrEMBL
Match: A0A438K320 (Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_009086 PE=4 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.8e-06
Identity = 49/129 (37.98%), Postives = 70/129 (54.26%), Query Frame = 0


Query: 6   SLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGF-RMDQL-KRSLDKSKSNEVSSV 65
           S  L LL LS  +HAC+AR L +      E  ++S      DQ+ K SL       + + 
Sbjct: 2   SSLLLLLLLSLSMHACNARHLGVIGKENREVHLLSKVVEEADQVHKISLPPRLIPSMETA 61

Query: 66  ENV-GIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQPHR 125
           E +   +IL G K+  RL +             D+++ V+S+E ++ ED+VVMDYAQPHR
Sbjct: 62  EKLQPQQILPGWKRHSRLML--------GSAPHDVEETVDSKEGDIVEDIVVMDYAQPHR 121

Query: 126 KPPIHNRKP 132
           KPPIHN+ P
Sbjct: 122 KPPIHNKDP 122

[Close]

BLAST of CmaCh06G007780 vs. ExPASy TrEMBL
Match: U5GMJ2 (Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_001G122600 PE=4 SV=1)

HSP 1 Score: 60.1 bits (144), Expect = 8.1e-06
Identity = 25/36 (69.44%), Postives = 31/36 (86.11%), Query Frame = 0


Query: 96  DIDKVVNSEENEVGEDMVVMDYAQPHRKPPIHNRKP 132
           D ++ V SEEN++ ED+ VMDYAQPHRKPPIHN+KP
Sbjct: 148 DTEEAVRSEENDIAEDVAVMDYAQPHRKPPIHNKKP 183

[Close]

BLAST of CmaCh06G007780 vs. NCBI nr
Match: XP_023005579.1 (uncharacterized protein LOC111498521 [Cucurbita maxima])

HSP 1 Score: 253.8 bits (647), Expect = 7.9e-64
Identity = 131/131 (100.00%), Postives = 131/131 (100.00%), Query Frame = 0


Query: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV 60
           MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV
Sbjct: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV 60

Query: 61  SSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP 120
           SSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP
Sbjct: 61  SSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP 120

Query: 121 HRKPPIHNRKP 132
           HRKPPIHNRKP
Sbjct: 121 HRKPPIHNRKP 131

[Close]

BLAST of CmaCh06G007780 vs. NCBI nr
Match: XP_023540680.1 (uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 231.5 bits (589), Expect = 4.2e-57
Identity = 120/131 (91.60%), Postives = 127/131 (96.95%), Query Frame = 0


Query: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV 60
           MPMPTS FLALLFLSTFLHACDARLLKLCHSSRLESC+ISPGFR +QL+RSLD+SKSN+V
Sbjct: 1   MPMPTSFFLALLFLSTFLHACDARLLKLCHSSRLESCLISPGFRTNQLERSLDESKSNDV 60

Query: 61  SSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP 120
           SS+ENVGIEILKGMKKLRRLAMELPVRDRDR  DKDIDKVVNSEENEVGEDMVVMDYAQP
Sbjct: 61  SSMENVGIEILKGMKKLRRLAMELPVRDRDR--DKDIDKVVNSEENEVGEDMVVMDYAQP 120

Query: 121 HRKPPIHNRKP 132
           HRKPPIHN+KP
Sbjct: 121 HRKPPIHNQKP 129

[Close]

BLAST of CmaCh06G007780 vs. NCBI nr
Match: KAG7028383.1 (Root meristem growth factor 6 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 225.7 bits (574), Expect = 2.3e-55
Identity = 117/131 (89.31%), Postives = 126/131 (96.18%), Query Frame = 0


Query: 1   MPMPTSLFLALLFLSTFLHACDARLLKLCHSSRLESCMISPGFRMDQLKRSLDKSKSNEV 60
           MPMPTSLFL+LLFLSTFLHACDARLLKLCHSSRLE CMISPGFR +QL++SLD+S+SN+V
Sbjct: 1   MPMPTSLFLSLLFLSTFLHACDARLLKLCHSSRLEPCMISPGFRTNQLEKSLDESRSNDV 60

Query: 61  SSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP 120
           SS+ENVGIEILKGMKKLRRLAMELPVRD DR  DKDIDKVVNSEENEVGEDMVVMDYAQP
Sbjct: 61  SSMENVGIEILKGMKKLRRLAMELPVRDIDR--DKDIDKVVNSEENEVGEDMVVMDYAQP 120

Query: 121 HRKPPIHNRKP 132
           HRKPPIHN+KP
Sbjct: 121 HRKPPIHNQKP 129

[Close]

BLAST of CmaCh06G007780 vs. NCBI nr
Match: KAG6596855.1 (Root meristem growth factor 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 158.3 bits (399), Expect = 4.5e-35
Identity = 82/94 (87.23%), Postives = 90/94 (95.74%), Query Frame = 0


Query: 38  MISPGFRMDQLKRSLDKSKSNEVSSVENVGIEILKGMKKLRRLAMELPVRDRDRDKDKDI 97
           MISPGFR +QL++SLD+S+SN+VSS+ENVGIEILKGMKKLRRLAMELPVRD DR  DKDI
Sbjct: 1   MISPGFRTNQLEKSLDESRSNDVSSMENVGIEILKGMKKLRRLAMELPVRDIDR--DKDI 60

Query: 98  DKVVNSEENEVGEDMVVMDYAQPHRKPPIHNRKP 132
           DKVVNSEENEVGEDMVVMDYAQPHRKPPIHN+KP
Sbjct: 61  DKVVNSEENEVGEDMVVMDYAQPHRKPPIHNQKP 92

[Close]

BLAST of CmaCh06G007780 vs. NCBI nr
Match: XP_038905997.1 (uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida])

HSP 1 Score: 114.4 bits (285), Expect = 7.5e-22
Identity = 73/131 (55.73%), Postives = 89/131 (67.94%), Query Frame = 0


Query: 7   LFLALLFLSTFLHACDARLLKLCHS----SRLESCMISPGFRMDQLKRSLDKSKSNEVSS 66
           LFL LL  S+ LHAC+AR L+   S    SR+E   IS G   DQ +RS ++S S+++S 
Sbjct: 4   LFLILLIFSSTLHACEARCLRFFESSSFVSRIEPHSISQGIWADQPERSFEESNSDKLSY 63

Query: 67  VE-NVGIEILKGMKKL-RRLAMELPVRDRDRDKDKDIDKVVNSEENEVGEDMVVMDYAQP 126
            E  VGIE+LKGMKK   RL ME P++          +KV+NS+ENEVGEDMVVMDYAQP
Sbjct: 64  KEIIVGIEMLKGMKKHGSRLVMEFPMK----------EKVLNSKENEVGEDMVVMDYAQP 123

Query: 127 HRKPPIHNRKP 132
           HRKPPIHN KP
Sbjct: 124 HRKPPIHNEKP 124

[Close]

BLAST of CmaCh06G007780 vs. TAIR 10
Match: AT4G16515.1 (Encodes a root meristem growth factor (RGF). Belongs to a family of functionally redundant homologous peptides that are secreted, tyrosine-sulfated, and expressed mainly in the stem cell area and the innermost layer of central columella cells. RGFs are required for maintenance of the root stem cell niche and transit amplifying cell proliferation. Members of this family include: At5g60810 (RGF1), At1g13620 (RGF2), At2g04025 (RGF3), At3g30350 (RGF4), At5g51451 (RGF5), At4g16515 (RGF6), At3g02240 (RGF7), At2g03830 (RGF8) and At5g64770 (RGF9). )

HSP 1 Score: 46.2 bits (108), Expect = 2.3e-05
Identity = 19/24 (79.17%), Postives = 21/24 (87.50%), Query Frame = 0


Query: 107 EVGEDMVVMDYAQPHRKPPIHNRK 131
           E GE++VVMDY QPHRKPPIHN K
Sbjct: 62  ETGEEVVVMDYPQPHRKPPIHNEK 85

The following BLAST results are available for this feature:

BLAST of CmaCh06G007780 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VK8	3.3e-04	79.17	Protein GOLVEN 1 OS=Arabidopsis thaliana OX=3702 GN=GLV1 PE=1 SV=1	[more]

BLAST of CmaCh06G007780 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KZM9	3.8e-64	100.00	uncharacterized protein LOC111498521 OS=Cucurbita maxima OX=3661 GN=LOC111498521...	[more]
A0A6J1DKL1	7.6e-20	52.59	uncharacterized protein LOC111021396 OS=Momordica charantia OX=3673 GN=LOC111021...	[more]
A0A0A0L298	7.8e-17	50.70	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G020610 PE=4 SV=1	[more]
A0A438K320	2.8e-06	37.98	Uncharacterized protein OS=Vitis vinifera OX=29760 GN=CK203_009086 PE=4 SV=1	[more]
U5GMJ2	8.1e-06	69.44	Uncharacterized protein OS=Populus trichocarpa OX=3694 GN=POPTR_001G122600 PE=4 ...	[more]

BLAST of CmaCh06G007780 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023005579.1	7.9e-64	100.00	uncharacterized protein LOC111498521 [Cucurbita maxima]	[more]
XP_023540680.1	4.2e-57	91.60	uncharacterized protein LOC111800973 [Cucurbita pepo subsp. pepo]	[more]
KAG7028383.1	2.3e-55	89.31	Root meristem growth factor 6 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6596855.1	4.5e-35	87.23	Root meristem growth factor 6, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_038905997.1	7.5e-22	55.73	uncharacterized protein LOC120091906 isoform X2 [Benincasa hispida]	[more]

BLAST of CmaCh06G007780 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G16515.1	2.3e-05	79.17	Encodes a root meristem growth factor (RGF). Belongs to a family of functionall...	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 110..131
None	No IPR available	PANTHER	PTHR34961	TRANSMEMBRANE PROTEIN	coord: 66..131
None	No IPR available	PANTHER	PTHR34961:SF7	SUBFAMILY NOT NAMED	coord: 66..131

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh06G007780.1	CmaCh06G007780.1	mRNA