CmaCh04G009270 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G009270
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	Cma_Chr04: 4780712 .. 4782735 (-)
RNA-Seq Expression	CmaCh04G009270
Synteny	CmaCh04G009270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTCTCTCTAACGAACTCCCATTCTCTCCAAAACAAAAAATGCCTGAAGCTCCAAACGCACCCTCTCTGGTGGAAAAAAACAGCGCCGCTCTCCAATTCATCGAACACATCACCACCAACGCCGCCCATGTGCAGCGCCAAGTCCTCTCCCAAATCCTCTCCCGCAATGCCTCCTCCGAGTACTTAACCCTCCACGCTCGCCCTTCCCCCGACACTTTCAAATCATCCATCCCTTTGATCTCTTACGACCACATCCAACCCTTCATCACCCGCATCGCCAATGGCGACACTTCCCCTATTCTCTGTTCTTCTCCCATCTCCGAGTTCTTGACCAGCTCCGGCACTTCCGGCGGCGAGAGAAAGCTGATGCCCACTATCCATGAAGAGCTCGACCGTCGCTCACTCTTGTACAGTTTGTTGATGCCTGTAATGAACCAGTTCATTCCGGGGTTGGAGAAGGGTAAAGGAATGTACTTTTTGTTCGTCAAAGCCGAGGCTCGAACCCCTGGCGGCCTTCTCGCTCGCCCTGTTTTAACTAGCTACTACAAAAGCTCCCACTTTAAGGACCGCCCCTACGATCCCTACACCAATTACACCAGCCCCAATGAGGCTATACTTTGTACGGACTCATACCAAAGCATGTACGCTCAAGTGCTGTGCGGCCTCTGCCACCGTCTCGATGTGCTACGCGTGGGCGCGGTTTTCGCCTCTGGCTTTATCCGCGCCATACGGTTTCTGGAGAAGCACTGGCAGCTACTCTGCCATGATATCCGGAGTGGGACGCTGAATTCCCAAATCACGGACCAGGCGGTTCGGGAGGCTGTACTGATGTCAGTCCTGCGTAGGCCTGACCCTGAGCTGGGGGACTTTATTGAGGGGGAGTGTTGTAAGGGGTCGTGGCAGGGAATCATTACAAGGCTGTGGCCTAATACCAAGTATGTGGATGTGATTGTAACAGGGGCAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGCCTTCCTTTGGTGTGTAGCATGTATGCTTCGTCCGAGTGCTATTTTGGTCTCAATCTTAACCCTCTTTGTAGCCCAAATGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTATTTTGAGTTTCTTCCTCTCCAAACAAACCACAATCACGAACACCAACTCGTCGATCTTACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTCGTCACAACATATGCTGGTAAGACCATTCTATTCAACTATTGTTTATGGAAGCCATGAAAATGAAATTATTTTGTTTCAATTCACAGGACTGTATCGATATCGAGTTGGGGACATTCTAAGAGTGGCTGGATTCAAAAACAACGCTCCTCAATTCAACTTCGTGTGCCGAAAGAATGTGGTTCTAAGCATCGACTCTGACAAGACCGATGAAGTTGAACTCCAAAATGCGGTGAAAAACTCCGTCAACCATTTGGTGCCATTCGACGCAACGTTAGTGGACTACACAAGCTACGCCAACACATCAACAATTCCGGGGCACTACGTGGTGTATTGGGAGGTGAACCAAAAGGGGTCGACCCGAGTGCCGCCGTCGGTGTTTGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCCATCGGCCCTTTGGAGATCAAGGTTGTCGAAATTGGGACATTTGATAAGCTCATGGATTATGCCATAACCATGGGAGCTTCTATAAATCAGTACAAAACGCCTAGATGTGTGAAGTTTGAGCCCATCGTTCAGCTTTTGAACTCGAGAGTGGTGGGTAGTTACTTCAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTGCCAACAGGATTCAACCTTCACTAATTAGGCAGATTTTTATGCGCTTTTTCTTTTTCTTCTTTTTTTTTTCCCCTTGCAGTCAAATAATATGTATGTTCTTGTGATAGATCATGTTAAAAGCATCATCAATTCACAACCTCCATAATTTTTAT

mRNA sequence

TCTCTCTCTAACGAACTCCCATTCTCTCCAAAACAAAAAATGCCTGAAGCTCCAAACGCACCCTCTCTGGTGGAAAAAAACAGCGCCGCTCTCCAATTCATCGAACACATCACCACCAACGCCGCCCATGTGCAGCGCCAAGTCCTCTCCCAAATCCTCTCCCGCAATGCCTCCTCCGAGTACTTAACCCTCCACGCTCGCCCTTCCCCCGACACTTTCAAATCATCCATCCCTTTGATCTCTTACGACCACATCCAACCCTTCATCACCCGCATCGCCAATGGCGACACTTCCCCTATTCTCTGTTCTTCTCCCATCTCCGAGTTCTTGACCAGCTCCGGCACTTCCGGCGGCGAGAGAAAGCTGATGCCCACTATCCATGAAGAGCTCGACCGTCGCTCACTCTTGTACAGTTTGTTGATGCCTGTAATGAACCAGTTCATTCCGGGGTTGGAGAAGGGTAAAGGAATGTACTTTTTGTTCGTCAAAGCCGAGGCTCGAACCCCTGGCGGCCTTCTCGCTCGCCCTGTTTTAACTAGCTACTACAAAAGCTCCCACTTTAAGGACCGCCCCTACGATCCCTACACCAATTACACCAGCCCCAATGAGGCTATACTTTGTACGGACTCATACCAAAGCATGTACGCTCAAGTGCTGTGCGGCCTCTGCCACCGTCTCGATGTGCTACGCGTGGGCGCGGTTTTCGCCTCTGGCTTTATCCGCGCCATACGGTTTCTGGAGAAGCACTGGCAGCTACTCTGCCATGATATCCGGAGTGGGACGCTGAATTCCCAAATCACGGACCAGGCGGTTCGGGAGGCTGTACTGATGTCAGTCCTGCGTAGGCCTGACCCTGAGCTGGGGGACTTTATTGAGGGGGAGTGTTGTAAGGGGTCGTGGCAGGGAATCATTACAAGGCTGTGGCCTAATACCAAGTATGTGGATGTGATTGTAACAGGGGCAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGCCTTCCTTTGGTGTGTAGCATGTATGCTTCGTCCGAGTGCTATTTTGGTCTCAATCTTAACCCTCTTTGTAGCCCAAATGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTATTTTGAGTTTCTTCCTCTCCAAACAAACCACAATCACGAACACCAACTCGTCGATCTTACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTCGTCACAACATATGCTGGACTGTATCGATATCGAGTTGGGGACATTCTAAGAGTGGCTGGATTCAAAAACAACGCTCCTCAATTCAACTTCGTGTGCCGAAAGAATGTGGTTCTAAGCATCGACTCTGACAAGACCGATGAAGTTGAACTCCAAAATGCGGTGAAAAACTCCGTCAACCATTTGGTGCCATTCGACGCAACGTTAGTGGACTACACAAGCTACGCCAACACATCAACAATTCCGGGGCACTACGTGGTGTATTGGGAGGTGAACCAAAAGGGGTCGACCCGAGTGCCGCCGTCGGTGTTTGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCCATCGGCCCTTTGGAGATCAAGGTTGTCGAAATTGGGACATTTGATAAGCTCATGGATTATGCCATAACCATGGGAGCTTCTATAAATCAGTACAAAACGCCTAGATGTGTGAAGTTTGAGCCCATCGTTCAGCTTTTGAACTCGAGAGTGGTGGGTAGTTACTTCAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTGCCAACAGGATTCAACCTTCACTAATTAGGCAGATTTTTATGCGCTTTTTCTTTTTCTTCTTTTTTTTTTCCCCTTGCAGTCAAATAATATGTATGTTCTTGTGATAGATCATGTTAAAAGCATCATCAATTCACAACCTCCATAATTTTTAT

Coding sequence (CDS)

ATGCCTGAAGCTCCAAACGCACCCTCTCTGGTGGAAAAAAACAGCGCCGCTCTCCAATTCATCGAACACATCACCACCAACGCCGCCCATGTGCAGCGCCAAGTCCTCTCCCAAATCCTCTCCCGCAATGCCTCCTCCGAGTACTTAACCCTCCACGCTCGCCCTTCCCCCGACACTTTCAAATCATCCATCCCTTTGATCTCTTACGACCACATCCAACCCTTCATCACCCGCATCGCCAATGGCGACACTTCCCCTATTCTCTGTTCTTCTCCCATCTCCGAGTTCTTGACCAGCTCCGGCACTTCCGGCGGCGAGAGAAAGCTGATGCCCACTATCCATGAAGAGCTCGACCGTCGCTCACTCTTGTACAGTTTGTTGATGCCTGTAATGAACCAGTTCATTCCGGGGTTGGAGAAGGGTAAAGGAATGTACTTTTTGTTCGTCAAAGCCGAGGCTCGAACCCCTGGCGGCCTTCTCGCTCGCCCTGTTTTAACTAGCTACTACAAAAGCTCCCACTTTAAGGACCGCCCCTACGATCCCTACACCAATTACACCAGCCCCAATGAGGCTATACTTTGTACGGACTCATACCAAAGCATGTACGCTCAAGTGCTGTGCGGCCTCTGCCACCGTCTCGATGTGCTACGCGTGGGCGCGGTTTTCGCCTCTGGCTTTATCCGCGCCATACGGTTTCTGGAGAAGCACTGGCAGCTACTCTGCCATGATATCCGGAGTGGGACGCTGAATTCCCAAATCACGGACCAGGCGGTTCGGGAGGCTGTACTGATGTCAGTCCTGCGTAGGCCTGACCCTGAGCTGGGGGACTTTATTGAGGGGGAGTGTTGTAAGGGGTCGTGGCAGGGAATCATTACAAGGCTGTGGCCTAATACCAAGTATGTGGATGTGATTGTAACAGGGGCAATGTCGCAGTATATTGGGACGCTTGATTATTATAGCAATGGCCTTCCTTTGGTGTGTAGCATGTATGCTTCGTCCGAGTGCTATTTTGGTCTCAATCTTAACCCTCTTTGTAGCCCAAATGAAGTTGCTTACACTCTCATCCCTTCCATGGCCTATTTTGAGTTTCTTCCTCTCCAAACAAACCACAATCACGAACACCAACTCGTCGATCTTACCGATGTCGAACTCGGCAAAGAATATGAGCTCGTCGTCACAACATATGCTGGACTGTATCGATATCGAGTTGGGGACATTCTAAGAGTGGCTGGATTCAAAAACAACGCTCCTCAATTCAACTTCGTGTGCCGAAAGAATGTGGTTCTAAGCATCGACTCTGACAAGACCGATGAAGTTGAACTCCAAAATGCGGTGAAAAACTCCGTCAACCATTTGGTGCCATTCGACGCAACGTTAGTGGACTACACAAGCTACGCCAACACATCAACAATTCCGGGGCACTACGTGGTGTATTGGGAGGTGAACCAAAAGGGGTCGACCCGAGTGCCGCCGTCGGTGTTTGAAGATTGTTGTCTTACGATGGAGGAGTCGCTGAACAGCGTATACCGTCAAGGGCGGGTGTCGGACAAGTCCATCGGCCCTTTGGAGATCAAGGTTGTCGAAATTGGGACATTTGATAAGCTCATGGATTATGCCATAACCATGGGAGCTTCTATAAATCAGTACAAAACGCCTAGATGTGTGAAGTTTGAGCCCATCGTTCAGCTTTTGAACTCGAGAGTGGTGGGTAGTTACTTCAGCCCTAAGTGCCCCAAGTGGGTTGCTGGTCATAAGCAATGGTGCCAACAGGATTCAACCTTCACTAATTAG

Protein sequence

MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYFEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN

Homology

[Close]

BLAST of CmaCh04G009270 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 937.9 bits (2423), Expect = 5.4e-272
Identity = 456/611 (74.63%), Postives = 519/611 (84.94%), Query Frame = 0


Query: 1   MPEAP-------NAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLH- 60
           MPEAP       +  SL EKN   LQFIE +TTNA  VQR+VL +ILSRNA  EYL  H 
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  --ARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 120
              R   +TFK  +P+++Y+ IQP I RIANGD S +LCS+PISEFLTSSGTSGGERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 PTIHEELDRRSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 180
           PTI EELDRRSLLYSLLMPVM+QF+PGL+KGKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFK+RPYDPYTNYTSPN+ ILC+DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 300
           +FLEKHW  L  DIR+GTL+S+ITD +VREAV    + +PDP+L DF+E EC K SWQGI
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAV--GEILKPDPKLADFVESECRKTSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 360
           ITRLWPNTKYVDVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NL PLC P+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPLQTN--------------HNHEHQLVDLTDVELGKEYELVVTTYA 420
           +YTLIP+MAYFEFLP+  N                 + +LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFD 480
           GLYRYRVGD+L VAGFKNNAPQF+F+CRKNVVLSIDSDKTDEVELQNAVKN+V HLVPFD
Sbjct: 421 GLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 ATLVDYTSYANTSTIPGHYVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSD 540
           A+L +YTSYA+TS+IPGHYV++WE+   G+T +PPSVFEDCCLT+EESLNSVYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSD 540

Query: 541 KSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPK 588
           KSIGPLEIK+VE GTFDKLMDYAI++GASINQYKTPRCVKF PI++LLNSRVV SYFSPK
Sbjct: 541 KSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

[Close]

BLAST of CmaCh04G009270 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 921.8 bits (2381), Expect = 4.0e-267
Identity = 451/611 (73.81%), Postives = 513/611 (83.96%), Query Frame = 0


Query: 1   MPEAPNAPSLV-------EKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLH- 60
           MPEAP   SL        +KN   LQ IE +T+NA  VQRQVL +IL+RNA  EYL  H 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  --ARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 120
              R   +TFK+ +P+I+Y+ I+P I RIANGD SPIL S PISEFLTSSGTSGGERKLM
Sbjct: 61  LNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIHEELDRRSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 180
           PTI EELDRRSLLYSLLMPVM+QF+PGLE GKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFK+RPYDPYTNYTSPNE ILC+DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAI 240

Query: 241 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 300
           +FLEKHW  L  DIR+GTL+S ITD +VREAV  + + +P P+L DF+E EC K SWQGI
Sbjct: 241 KFLEKHWIELVRDIRTGTLSSLITDPSVREAV--AKILKPSPKLADFVEFECKKSSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 360
           ITRLWPNTKYVDVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NL PLC P+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPLQTNH--------------NHEHQLVDLTDVELGKEYELVVTTYA 420
           +YTLIPSMAYFEFLP+  N+                + +LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFD 480
           GL RYRVGD+LRV GFKN APQF+F+CRKNVVLSIDSDKTDEVELQNAVKN+V HLVPFD
Sbjct: 421 GLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 ATLVDYTSYANTSTIPGHYVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSD 540
           A+L +YTSYA+TS+IPGHYV++WE+   G+T +PPSVFEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 541 KSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPK 588
           KSIGPLEIK+VE GTFDKLMDYAI++GASINQYKTPRCVKF PI++LLNSRVV SYFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

[Close]

BLAST of CmaCh04G009270 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 832.4 bits (2149), Expect = 3.2e-240
Identity = 407/601 (67.72%), Postives = 483/601 (80.37%), Query Frame = 0


Query: 1   MPEAPNAPSLVE------KNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHAR 60
           MPEAP A +          ++ AL+FIEH+T NA  VQR+VL +IL++NA +EYL  +  
Sbjct: 1   MPEAPTAKTAPAYGYAPGAHAEALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGI 60

Query: 61  P-SP---DTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 120
           P SP   D F+  +PL++Y+ +QP I RIANGDTSPI    PISEFLTSSGTSGGERKLM
Sbjct: 61  PGSPDVVDAFRRLVPLVTYEGLQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIHEELDRRSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 180
           PTI +E++RRSLLYSLLMPVM+Q + GL+KGK MY LFVKAE+RTPGGL ARPVLTSYY+
Sbjct: 121 PTIADEMNRRSLLYSLLMPVMSQSVSGLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYR 180

Query: 181 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 240
           S  F DRP DPYT+YTSP+EAILC DSYQSMYAQ+LCGL HR DVLRVGAVFASGF+RAI
Sbjct: 181 SRQFLDRPRDPYTSYTSPDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAI 240

Query: 241 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 300
            FLEKHW  LCHDIR+G L+ +ITD+ VR+AV    + R DP L D IE EC + SW+GI
Sbjct: 241 HFLEKHWARLCHDIRTGELDPEITDRVVRDAV--GRVLRADPALADAIEDECARASWEGI 300

Query: 301 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 360
           I RLWP TKY+DVIVTG MSQYI TL++Y  GLPL C+MYASSECYFGLNLNP+C P++V
Sbjct: 301 IRRLWPRTKYIDVIVTGTMSQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDV 360

Query: 361 AYTLIPSMAYFEFLPLQTNH----NHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDI 420
           AYTLIP+M Y+EFLP+  N+         LVDL DV+LG EYELVVTTY+GLYRYRVGD+
Sbjct: 361 AYTLIPTMCYYEFLPVNCNNATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDV 420

Query: 421 LRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYA 480
           LRVAGFKN AP F+FV R+NV LS+DSDKTDE EL  AV  +V HL PF A+LV+YTSYA
Sbjct: 421 LRVAGFKNKAPMFSFVRRQNVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYA 480

Query: 481 NTSTIPGHYVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKV 540
           + +TIPGHYV++WE+ + GST VP SVFE+CCL++EE+LNSVYRQGR  D+SIGPLEI+V
Sbjct: 481 DAATIPGHYVLFWEL-RAGSTAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRV 540

Query: 541 VEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQ 588
           V  GTFDKLMDYAI+ GASINQYK PRCV+  P+V+LL++RV G YFSPKCPKW  G+KQ
Sbjct: 541 VAEGTFDKLMDYAISRGASINQYKAPRCVRPGPVVELLDARVQGKYFSPKCPKWSPGNKQ 598

[Close]

BLAST of CmaCh04G009270 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 794.7 bits (2051), Expect = 7.4e-229
Identity = 395/601 (65.72%), Postives = 474/601 (78.87%), Query Frame = 0


Query: 2   PEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARP-----S 61
           P A  A ++  ++   L+ IE +T NA   QR+VL +IL++NA +EYL     P     +
Sbjct: 11  PAAAAATAMCAEHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGA 70

Query: 62  PDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEE 121
            + F+   PL++Y+ I P + RIANGDTSPIL   P+SEFLTSSGTSGGERKLMPTI EE
Sbjct: 71  DEAFRRLAPLVTYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEE 130

Query: 122 LDRRSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKD 181
           ++RRS LYSLLMPVM++ +PGL+KGK MY  FVK+E RTPGGL ARPVLTS+Y+S +F +
Sbjct: 131 MERRSGLYSLLMPVMSRQVPGLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYRSRYFLE 190

Query: 182 RPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKH 241
           RP+DPYT YTSP+EA+LC D+YQSMYAQ++CGL HR DVLRVGAVFASGF+RAIRFLEKH
Sbjct: 191 RPHDPYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKH 250

Query: 242 WQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWP 301
           W  LC DIR+G L+  +TD AVR AV   VLR  DP L D IE EC + SWQGII R+WP
Sbjct: 251 WPSLCRDIRAGELDGGVTDPAVRGAV-GRVLRGADPALADAIEAECARPSWQGIIRRVWP 310

Query: 302 NTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIP 361
           +TKY+DVIVTGAM+QYI TL++Y  GLPL C+MYASSECYFGLNLNP+C P+EVAYTLIP
Sbjct: 311 STKYIDVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIP 370

Query: 362 SMAYFEFLPLQTNHNH------EHQ-LVDLTDVELGKEYELVVTTYAGLYRYRVGDILRV 421
           +M YFEFLP+ +  N       +H+ LVDL DV+LG EYELVVTTY+GLYRYRVGD+LRV
Sbjct: 371 TMCYFEFLPVNSGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRV 430

Query: 422 AGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANT- 481
           AGFKN AP F FV RKNV LSIDSDKTDE EL  AV  +V HL PF A+LV+YTSYA+T 
Sbjct: 431 AGFKNAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTA 490

Query: 482 STIPGHYVVYWEV-NQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVV 541
           +TIPGHYV++WE+ +  G T VP SVFEDCCL +EE LNSVYRQ R +D+SIGPLEI+VV
Sbjct: 491 TTIPGHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVV 550

Query: 542 EIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVA-GHKQ 588
             GTFDKLMDYA++ GASINQYK PRCV+  P+V+LL+ RV  +YFSPKCPKW A G+KQ
Sbjct: 551 ADGTFDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQ 610

[Close]

BLAST of CmaCh04G009270 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 768.1 bits (1982), Expect = 7.4e-221
Identity = 381/582 (65.46%), Postives = 457/582 (78.52%), Query Frame = 0


Query: 4   APNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYL---TLHARPSPDTF 63
           +P  P   EK++ AL+FIE +T NA  VQ  +L++IL+RNA +EYL    L      DTF
Sbjct: 9   SPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTF 68

Query: 64  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 123
           K+ IP+I+Y+ +QP I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI EELDRR
Sbjct: 69  KTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRR 128

Query: 124 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 183
            LLYSLLMPVMN ++PGL+KGKGMYFLFVK+E +TPGGL ARPVLTSYYKS HF+ RPYD
Sbjct: 129 QLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYD 188

Query: 184 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 243
           PY  YTSPNEAILC DS+QSMY Q+LCGL  RL VLRVGAVFASG +RAIRFL+ HW   
Sbjct: 189 PYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRF 248

Query: 244 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 303
            HDI  G L+S+ITD ++R+   MS + +PDP L +FI  EC   +W+ IITR+WPNTKY
Sbjct: 249 AHDIELGCLDSEITDPSIRQC--MSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKY 308

Query: 304 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 363
           +DVIVTGAM+QYI TL+YYS GLP+ C+MYASSECYFGLNLNP+  P+EV+YT++P+MAY
Sbjct: 309 LDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAY 368

Query: 364 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 423
           FEF+PL        + V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N+APQF+
Sbjct: 369 FEFIPLGGT-----KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFH 428

Query: 424 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHL-VPFDATLVDYTSYANTSTIPGHYVVYW 483
           FV RKNV+LSIDSDKTDE ELQ AV+N+ + L     + + +YTSYA+TSTIPGHYV+YW
Sbjct: 429 FVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYW 488

Query: 484 EVNQKGSTRVPP-SVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDY 543
           E+  +   R P       CCL MEESLNSVYRQ RV+D S+GPLEI+VV  GTF++LMDY
Sbjct: 489 ELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDY 548

Query: 544 AITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKW 581
           AI+ GASINQYK PRCV F PIV+LL+SRVV ++FSP  P W
Sbjct: 549 AISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

[Close]

BLAST of CmaCh04G009270 vs. ExPASy TrEMBL
Match: A0A6J1J981 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=LOC111483735 PE=3 SV=1)

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0


Query: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60
           MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180
           SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420

Query: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480
           FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540
           VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 597
           TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

[Close]

BLAST of CmaCh04G009270 vs. ExPASy TrEMBL
Match: A0A6J1FR29 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111447677 PE=3 SV=1)

HSP 1 Score: 1217.6 bits (3149), Expect = 0.0e+00
Identity = 592/596 (99.33%), Postives = 593/596 (99.50%), Query Frame = 0


Query: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60
           MPEAPNAPSL+EKNSAALQFIE ITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180
           SLLYSLLMPVM QFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN APQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 420

Query: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480
           FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540
           VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 597
           TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

[Close]

BLAST of CmaCh04G009270 vs. ExPASy TrEMBL
Match: A0A5D3BYD3 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold68G00850 PE=3 SV=1)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 522/598 (87.29%), Postives = 561/598 (93.81%), Query Frame = 0


Query: 2   PEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTFK 61
           P  PN   LV+KN A LQFIE +TTNAA VQRQVLSQILS+N++SEYLTL+ RPSPDTFK
Sbjct: 7   PSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRPSPDTFK 66

Query: 62  SSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRS 121
           +SIPL+SYD IQPF++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTI EEL+RRS
Sbjct: 67  TSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRS 126

Query: 122 LLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDP 181
           LLYSLLMPVM+QF+PGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSSHFK+RPYDP
Sbjct: 127 LLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDP 186

Query: 182 YTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 241
           YTNYTSPNEAILC DSYQSMYAQ+LCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC
Sbjct: 187 YTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 246

Query: 242 HDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYV 301
           HDIR+GTLNSQITDQAVR+AV++SVLRRPDPEL D+I GECCKGSWQGIITRLWPNTKYV
Sbjct: 247 HDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYV 306

Query: 302 DVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYF 361
           DVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYTLIPSMAYF
Sbjct: 307 DVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYF 366

Query: 362 EFLPLQTNHNH------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 421
           EFLP++ +HN+      E QLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 367 EFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 426

Query: 422 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHY 481
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATL +YTSYANTSTIPGHY
Sbjct: 427 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHY 486

Query: 482 VVYWEVNQK--GSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFD 541
           V+YWEVNQK   +T VPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE GTFD
Sbjct: 487 VLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFD 546

Query: 542 KLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 592
           KLMDYAI+MGASINQYKTPRCVKF+PIV+LLNSRVVGSYFSPKCPKWV GHKQW QQD
Sbjct: 547 KLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

[Close]

BLAST of CmaCh04G009270 vs. ExPASy TrEMBL
Match: A0A1S3BTN3 (indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493400 PE=3 SV=1)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 522/598 (87.29%), Postives = 561/598 (93.81%), Query Frame = 0


Query: 2   PEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTFK 61
           P  PN   LV+KN A LQFIE +TTNAA VQRQVLSQILS+N++SEYLTL+ RPSPDTFK
Sbjct: 7   PSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRPSPDTFK 66

Query: 62  SSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRS 121
           +SIPL+SYD IQPF++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTI EEL+RRS
Sbjct: 67  TSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRS 126

Query: 122 LLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDP 181
           LLYSLLMPVM+QF+PGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSSHFK+RPYDP
Sbjct: 127 LLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDP 186

Query: 182 YTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 241
           YTNYTSPNEAILC DSYQSMYAQ+LCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC
Sbjct: 187 YTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 246

Query: 242 HDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYV 301
           HDIR+GTLNSQITDQAVR+AV++SVLRRPDPEL D+I GECCKGSWQGIITRLWPNTKYV
Sbjct: 247 HDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYV 306

Query: 302 DVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYF 361
           DVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYTLIPSMAYF
Sbjct: 307 DVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYF 366

Query: 362 EFLPLQTNHNH------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 421
           EFLP++ +HN+      E QLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 367 EFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 426

Query: 422 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHY 481
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATL +YTSYANTSTIPGHY
Sbjct: 427 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHY 486

Query: 482 VVYWEVNQK--GSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFD 541
           V+YWEVNQK   +T VPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE GTFD
Sbjct: 487 VLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFD 546

Query: 542 KLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 592
           KLMDYAI+MGASINQYKTPRCVKF+PIV+LLNSRVVGSYFSPKCPKWV GHKQW QQD
Sbjct: 547 KLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

[Close]

BLAST of CmaCh04G009270 vs. ExPASy TrEMBL
Match: A0A6J1EW65 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111438458 PE=3 SV=1)

HSP 1 Score: 1070.5 bits (2767), Expect = 2.7e-309
Identity = 522/605 (86.28%), Postives = 562/605 (92.89%), Query Frame = 0


Query: 1   MPEAPNAP-------SLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHA 60
           MPEAP  P       +LV+KN+A LQFIE +TTNA  VQRQVLSQILSRNA+SEYL+LHA
Sbjct: 1   MPEAPKTPTSVTPNYNLVQKNNAILQFIEDVTTNADLVQRQVLSQILSRNATSEYLSLHA 60

Query: 61  RPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTI 120
           RPSPDTFKSSIPL+SYDHIQPFI+RIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTI
Sbjct: 61  RPSPDTFKSSIPLVSYDHIQPFISRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTI 120

Query: 121 HEELDRRSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSH 180
            EEL+RRSLLYSLLMP+M QF+PGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSH
Sbjct: 121 EEELERRSLLYSLLMPIMTQFVPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSH 180

Query: 181 FKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFL 240
           FK+RPYDPYTNYTSPNEAILC D+YQS+YAQ+LCGLC+RLDVLRVGAVFASGFIRAIRFL
Sbjct: 181 FKNRPYDPYTNYTSPNEAILCPDAYQSIYAQLLCGLCYRLDVLRVGAVFASGFIRAIRFL 240

Query: 241 EKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITR 300
           EKHWQLLCHDIR+GTLNSQITDQAV +AV++ VLRRPDPEL DFI  +C KG W+GIITR
Sbjct: 241 EKHWQLLCHDIRTGTLNSQITDQAVGDAVML-VLRRPDPELADFIHEQCSKGYWKGIITR 300

Query: 301 LWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYT 360
           LWPNTKY+DVIVTG MSQYIGTLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYT
Sbjct: 301 LWPNTKYLDVIVTGTMSQYIGTLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYT 360

Query: 361 LIPSMAYFEFLPLQTNHN-----HEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420
           LIPSMAYFEFLP+Q   N     ++ QLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR
Sbjct: 361 LIPSMAYFEFLPVQRTSNNGESLNDQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILR 420

Query: 421 VAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANT 480
           VAGFKN APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPF+ATL +YTS+ANT
Sbjct: 421 VAGFKNKAPQFNFICRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFEATLAEYTSHANT 480

Query: 481 STIPGHYVVYWEVNQKGS--TRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKV 540
           STIPGHYV+YWE+NQKGS  T VPPSV EDCCLT+EESLNSVYRQGRV+DKSIGPLEIKV
Sbjct: 481 STIPGHYVLYWELNQKGSSTTPVPPSVLEDCCLTIEESLNSVYRQGRVADKSIGPLEIKV 540

Query: 541 VEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQ 592
           VE GTFDKLMDYAI+MGASINQYKTPRCVKF+PIV+LLNSRVVG YFSPKCPKWVAGHKQ
Sbjct: 541 VESGTFDKLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGCYFSPKCPKWVAGHKQ 600

[Close]

BLAST of CmaCh04G009270 vs. NCBI nr
Match: XP_022985796.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima])

HSP 1 Score: 1226.8 bits (3173), Expect = 0.0e+00
Identity = 596/596 (100.00%), Postives = 596/596 (100.00%), Query Frame = 0


Query: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60
           MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180
           SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420

Query: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480
           FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540
           VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 597
           TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

[Close]

BLAST of CmaCh04G009270 vs. NCBI nr
Match: KAG6600750.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1219.5 bits (3154), Expect = 0.0e+00
Identity = 592/596 (99.33%), Postives = 594/596 (99.66%), Query Frame = 0


Query: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60
           MPEAPNAPSL+EKNSAALQFIE ITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180
           SLLYSLLMPVM QFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420

Query: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480
           FVCRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540
           VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 597
           TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

[Close]

BLAST of CmaCh04G009270 vs. NCBI nr
Match: XP_022942727.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 1217.6 bits (3149), Expect = 0.0e+00
Identity = 592/596 (99.33%), Postives = 593/596 (99.50%), Query Frame = 0


Query: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60
           MPEAPNAPSL+EKNSAALQFIE ITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180
           SLLYSLLMPVM QFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN APQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNKAPQFN 420

Query: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480
           FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540
           VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 597
           TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 596

[Close]

BLAST of CmaCh04G009270 vs. NCBI nr
Match: XP_023511762.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1214.1 bits (3140), Expect = 0.0e+00
Identity = 590/596 (98.99%), Postives = 592/596 (99.33%), Query Frame = 0


Query: 1   MPEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60
           MPEAPNAPSL+EKNSAALQFIE ITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF
Sbjct: 1   MPEAPNAPSLLEKNSAALQFIEDITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTF 60

Query: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120
           KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR
Sbjct: 61  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 120

Query: 121 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180
           SLLYSLLMPVM QFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD
Sbjct: 121 SLLYSLLMPVMTQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 180

Query: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240
           PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL
Sbjct: 181 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 240

Query: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300
           CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY
Sbjct: 241 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 300

Query: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360
           VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY
Sbjct: 301 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 360

Query: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420
           FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN
Sbjct: 361 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 420

Query: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480
           FVCRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPFDATLVDYTSYANTSTIPGHYVVYWE
Sbjct: 421 FVCRKNVVLSIDSDKTDEVELQNAVKNSINHLVPFDATLVDYTSYANTSTIPGHYVVYWE 480

Query: 481 VNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540
           VNQKGST VPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI
Sbjct: 481 VNQKGSTGVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDYAI 540

Query: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQDSTFTN 597
           TMGASINQYKTPRCVKFEPIVQLLNSRVV SYFSPKCPKWVAGHKQWCQQDSTFTN
Sbjct: 541 TMGASINQYKTPRCVKFEPIVQLLNSRVVDSYFSPKCPKWVAGHKQWCQQDSTFTN 596

[Close]

BLAST of CmaCh04G009270 vs. NCBI nr
Match: XP_008452341.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA0047007.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa] >TYK04773.1 indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo var. makuwa])

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 522/598 (87.29%), Postives = 561/598 (93.81%), Query Frame = 0


Query: 2   PEAPNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLHARPSPDTFK 61
           P  PN   LV+KN A LQFIE +TTNAA VQRQVLSQILS+N++SEYLTL+ RPSPDTFK
Sbjct: 7   PSTPNYNQLVQKNKAILQFIEDVTTNAALVQRQVLSQILSQNSNSEYLTLYGRPSPDTFK 66

Query: 62  SSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRS 121
           +SIPL+SYD IQPF++RIANGD SPILCSSPISEFLTSSGTSGGERKLMPTI EEL+RRS
Sbjct: 67  TSIPLVSYDQIQPFVSRIANGDFSPILCSSPISEFLTSSGTSGGERKLMPTIEEELERRS 126

Query: 122 LLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDP 181
           LLYSLLMPVM+QF+PGLEKGKGMYFLF+KAEA+TPGGLLARPVLTSYYKSSHFK+RPYDP
Sbjct: 127 LLYSLLMPVMSQFVPGLEKGKGMYFLFIKAEAKTPGGLLARPVLTSYYKSSHFKERPYDP 186

Query: 182 YTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 241
           YTNYTSPNEAILC DSYQSMYAQ+LCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC
Sbjct: 187 YTNYTSPNEAILCPDSYQSMYAQLLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLC 246

Query: 242 HDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYV 301
           HDIR+GTLNSQITDQAVR+AV++SVLRRPDPEL D+I GECCKGSWQGIITRLWPNTKYV
Sbjct: 247 HDIRTGTLNSQITDQAVRDAVMLSVLRRPDPELADYIHGECCKGSWQGIITRLWPNTKYV 306

Query: 302 DVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYF 361
           DVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NLNPLC P+EVAYTLIPSMAYF
Sbjct: 307 DVIVTGTMSQYIATLDYYSNGLPLVCTMYASSECYFGVNLNPLCKPSEVAYTLIPSMAYF 366

Query: 362 EFLPLQTNHNH------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNN 421
           EFLP++ +HN+      E QLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKN 
Sbjct: 367 EFLPIERSHNNDDNSLDEQQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNK 426

Query: 422 APQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHY 481
           APQFNF+CRKNVVLSIDSDKTDEVELQNAVKNS+NHLVPF+ATL +YTSYANTSTIPGHY
Sbjct: 427 APQFNFICRKNVVLSIDSDKTDEVELQNAVKNSMNHLVPFEATLAEYTSYANTSTIPGHY 486

Query: 482 VVYWEVNQK--GSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFD 541
           V+YWEVNQK   +T VPPSV EDCCLT+EESLNSVYRQGRVSDKSIGPLEIKVVE GTFD
Sbjct: 487 VLYWEVNQKEGAATPVPPSVLEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKVVENGTFD 546

Query: 542 KLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKWVAGHKQWCQQD 592
           KLMDYAI+MGASINQYKTPRCVKF+PIV+LLNSRVVGSYFSPKCPKWV GHKQW QQD
Sbjct: 547 KLMDYAISMGASINQYKTPRCVKFQPIVELLNSRVVGSYFSPKCPKWVPGHKQWSQQD 604

[Close]

BLAST of CmaCh04G009270 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 937.9 bits (2423), Expect = 3.9e-273
Identity = 456/611 (74.63%), Postives = 519/611 (84.94%), Query Frame = 0


Query: 1   MPEAP-------NAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLH- 60
           MPEAP       +  SL EKN   LQFIE +TTNA  VQR+VL +ILSRNA  EYL  H 
Sbjct: 1   MPEAPKIAALEVSDESLAEKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHG 60

Query: 61  --ARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 120
              R   +TFK  +P+++Y+ IQP I RIANGD S +LCS+PISEFLTSSGTSGGERKLM
Sbjct: 61  LEGRTDRETFKHIMPVVTYEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLM 120

Query: 121 PTIHEELDRRSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 180
           PTI EELDRRSLLYSLLMPVM+QF+PGL+KGKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMDQFVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFK+RPYDPYTNYTSPN+ ILC+DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKNRPYDPYTNYTSPNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAI 240

Query: 241 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 300
           +FLEKHW  L  DIR+GTL+S+ITD +VREAV    + +PDP+L DF+E EC K SWQGI
Sbjct: 241 KFLEKHWPELARDIRTGTLSSEITDSSVREAV--GEILKPDPKLADFVESECRKTSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 360
           ITRLWPNTKYVDVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NL PLC P+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPLQTN--------------HNHEHQLVDLTDVELGKEYELVVTTYA 420
           +YTLIP+MAYFEFLP+  N                 + +LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPNMAYFEFLPVHRNSGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFD 480
           GLYRYRVGD+L VAGFKNNAPQF+F+CRKNVVLSIDSDKTDEVELQNAVKN+V HLVPFD
Sbjct: 421 GLYRYRVGDVLSVAGFKNNAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 ATLVDYTSYANTSTIPGHYVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSD 540
           A+L +YTSYA+TS+IPGHYV++WE+   G+T +PPSVFEDCCLT+EESLNSVYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSD 540

Query: 541 KSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPK 588
           KSIGPLEIK+VE GTFDKLMDYAI++GASINQYKTPRCVKF PI++LLNSRVV SYFSPK
Sbjct: 541 KSIGPLEIKMVESGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

[Close]

BLAST of CmaCh04G009270 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 921.8 bits (2381), Expect = 2.9e-268
Identity = 451/611 (73.81%), Postives = 513/611 (83.96%), Query Frame = 0


Query: 1   MPEAPNAPSLV-------EKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLTLH- 60
           MPEAP   SL        +KN   LQ IE +T+NA  VQRQVL +IL+RNA  EYL  H 
Sbjct: 1   MPEAPKKESLEVFDLTLDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHD 60

Query: 61  --ARPSPDTFKSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLM 120
              R   +TFK+ +P+I+Y+ I+P I RIANGD SPIL S PISEFLTSSGTSGGERKLM
Sbjct: 61  LNGRTDRETFKNIMPVITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLM 120

Query: 121 PTIHEELDRRSLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYK 180
           PTI EELDRRSLLYSLLMPVM+QF+PGLE GKGMYFLF+K+E++TPGGL ARPVLTSYYK
Sbjct: 121 PTIEEELDRRSLLYSLLMPVMSQFVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYK 180

Query: 181 SSHFKDRPYDPYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAI 240
           SSHFK+RPYDPYTNYTSPNE ILC+DSYQSMY+Q+LCGLC   +VLRVGAVFASGFIRAI
Sbjct: 181 SSHFKERPYDPYTNYTSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAI 240

Query: 241 RFLEKHWQLLCHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGI 300
           +FLEKHW  L  DIR+GTL+S ITD +VREAV  + + +P P+L DF+E EC K SWQGI
Sbjct: 241 KFLEKHWIELVRDIRTGTLSSLITDPSVREAV--AKILKPSPKLADFVEFECKKSSWQGI 300

Query: 301 ITRLWPNTKYVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEV 360
           ITRLWPNTKYVDVIVTG MSQYI TLDYYSNGLPLVC+MYASSECYFG+NL PLC P+EV
Sbjct: 301 ITRLWPNTKYVDVIVTGTMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEV 360

Query: 361 AYTLIPSMAYFEFLPLQTNH--------------NHEHQLVDLTDVELGKEYELVVTTYA 420
           +YTLIPSMAYFEFLP+  N+                + +LVDL DV+LG+EYELVVTTYA
Sbjct: 361 SYTLIPSMAYFEFLPVHRNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYA 420

Query: 421 GLYRYRVGDILRVAGFKNNAPQFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFD 480
           GL RYRVGD+LRV GFKN APQF+F+CRKNVVLSIDSDKTDEVELQNAVKN+V HLVPFD
Sbjct: 421 GLCRYRVGDLLRVTGFKNKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFD 480

Query: 481 ATLVDYTSYANTSTIPGHYVVYWEVNQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSD 540
           A+L +YTSYA+TS+IPGHYV++WE+   G+T +PPSVFEDCCL +EES N+VYRQGRVSD
Sbjct: 481 ASLSEYTSYADTSSIPGHYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSD 540

Query: 541 KSIGPLEIKVVEIGTFDKLMDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPK 588
           KSIGPLEIK+VE GTFDKLMDYAI++GASINQYKTPRCVKF PI++LLNSRVV SYFSPK
Sbjct: 541 KSIGPLEIKIVEPGTFDKLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPK 600

[Close]

BLAST of CmaCh04G009270 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 768.1 bits (1982), Expect = 5.3e-222
Identity = 381/582 (65.46%), Postives = 457/582 (78.52%), Query Frame = 0


Query: 4   APNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYL---TLHARPSPDTF 63
           +P  P   EK++ AL+FIE +T NA  VQ  +L++IL+RNA +EYL    L      DTF
Sbjct: 9   SPLGPPACEKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTF 68

Query: 64  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 123
           K+ IP+I+Y+ +QP I RIA+GD SPIL + PISEFLTSSGTS GERKLMPTI EELDRR
Sbjct: 69  KTKIPVITYEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRR 128

Query: 124 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 183
            LLYSLLMPVMN ++PGL+KGKGMYFLFVK+E +TPGGL ARPVLTSYYKS HF+ RPYD
Sbjct: 129 QLLYSLLMPVMNLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYD 188

Query: 184 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 243
           PY  YTSPNEAILC DS+QSMY Q+LCGL  RL VLRVGAVFASG +RAIRFL+ HW   
Sbjct: 189 PYNVYTSPNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRF 248

Query: 244 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKY 303
            HDI  G L+S+ITD ++R+   MS + +PDP L +FI  EC   +W+ IITR+WPNTKY
Sbjct: 249 AHDIELGCLDSEITDPSIRQC--MSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKY 308

Query: 304 VDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAY 363
           +DVIVTGAM+QYI TL+YYS GLP+ C+MYASSECYFGLNLNP+  P+EV+YT++P+MAY
Sbjct: 309 LDVIVTGAMAQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAY 368

Query: 364 FEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQFN 423
           FEF+PL        + V+L DV +GKEYELVVTTYAGL RYRVGDILRV GF N+APQF+
Sbjct: 369 FEFIPLGGT-----KAVELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFH 428

Query: 424 FVCRKNVVLSIDSDKTDEVELQNAVKNSVNHL-VPFDATLVDYTSYANTSTIPGHYVVYW 483
           FV RKNV+LSIDSDKTDE ELQ AV+N+ + L     + + +YTSYA+TSTIPGHYV+YW
Sbjct: 429 FVRRKNVLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYW 488

Query: 484 EVNQKGSTRVPP-SVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDY 543
           E+  +   R P       CCL MEESLNSVYRQ RV+D S+GPLEI+VV  GTF++LMDY
Sbjct: 489 ELLVRDGARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDY 548

Query: 544 AITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKW 581
           AI+ GASINQYK PRCV F PIV+LL+SRVV ++FSP  P W
Sbjct: 549 AISRGASINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLPHW 583

[Close]

BLAST of CmaCh04G009270 vs. TAIR 10
Match: AT4G37390.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 751.1 bits (1938), Expect = 6.7e-217
Identity = 366/585 (62.56%), Postives = 456/585 (77.95%), Query Frame = 0


Query: 12  EKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYL---TLHARPSPDTFKSSIPLIS 71
           EK+  AL+FIE +T N   VQ +VL +IL+RN+++EYL    L       TFKS +P+++
Sbjct: 18  EKDVKALKFIEEMTRNPDSVQEKVLGEILTRNSNTEYLKRFDLDGVVDRKTFKSKVPVVT 77

Query: 72  YDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRRSLLYSLLM 131
           Y+ ++P I RI+NGD SPIL S PI+EFLTSSGTS GERKLMPTI E+LDRR LLYSLLM
Sbjct: 78  YEDLKPEIQRISNGDCSPILSSHPITEFLTSSGTSAGERKLMPTIEEDLDRRQLLYSLLM 137

Query: 132 PVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYDPYTNYTSP 191
           PVMN ++PGL+KGKG+YFLFVK+E++T GGL ARPVLTSYYKS HFK RPYDPY  YTSP
Sbjct: 138 PVMNLYVPGLDKGKGLYFLFVKSESKTSGGLPARPVLTSYYKSDHFKRRPYDPYNVYTSP 197

Query: 192 NEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLLCHDIRSGT 251
           NEAILC+DS QSMYAQ+LCGL  R +VLR+GAVFASG +RAI FL+ +W+ L  DI +GT
Sbjct: 198 NEAILCSDSSQSMYAQMLCGLLMRHEVLRLGAVFASGLLRAISFLQNNWKELARDISTGT 257

Query: 252 LNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCKGSWQGIITRLWPNTKYVDVIVTGA 311
           L+S+I D A++   +  +L +PD EL +F+ G C + +W+GIIT++WPNTKY+DVIVTGA
Sbjct: 258 LSSRIFDPAIKNR-MSKILTKPDQELAEFLVGVCSQENWEGIITKIWPNTKYLDVIVTGA 317

Query: 312 MSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMAYFEFLPLQT 371
           M+QYI TL+YYS GLP+ C+MYASSE YFG+NL P+C P+EV+YT++P+MAYFEFLP   
Sbjct: 318 MAQYIPTLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP--- 377

Query: 372 NHNH-----------EHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAP 431
            HNH           E  LV+L +VE+GKEYELV+TTYAGLYRYRVGDILRV GF N+AP
Sbjct: 378 -HNHDGDGAAEASLDETSLVELANVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAP 437

Query: 432 QFNFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVV 491
           QF F+ RKNV+LS++SDKTDE ELQ AV+N+          +++YTSYA T TIPGHYV+
Sbjct: 438 QFKFIRRKNVLLSVESDKTDEAELQKAVENASRLFAEQGTRVIEYTSYAETKTIPGHYVI 497

Query: 492 YWEV--NQKGSTRVPPSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKL 551
           YWE+    + +  +   V   CCL MEESLNSVYRQ RV+DKSIGPLEI+VV  GTF++L
Sbjct: 498 YWELLGRDQSNALMSEEVMAKCCLEMEESLNSVYRQSRVADKSIGPLEIRVVRNGTFEEL 557

Query: 552 MDYAITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKW 581
           MDYAI+ GASINQYK PRCV F PI++LL+SRVV ++FSP  P W
Sbjct: 558 MDYAISRGASINQYKVPRCVSFTPIMELLDSRVVSAHFSPSLPHW 597

[Close]

BLAST of CmaCh04G009270 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 736.9 bits (1901), Expect = 1.3e-212
Identity = 366/582 (62.89%), Postives = 452/582 (77.66%), Query Frame = 0


Query: 4   APNAPSLVEKNSAALQFIEHITTNAAHVQRQVLSQILSRNASSEYLT---LHARPSPDTF 63
           +P   S   K+  AL+FIE +T N   VQ++V+ +ILSRN+ +EYL    L       TF
Sbjct: 9   SPMMHSPSTKDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTF 68

Query: 64  KSSIPLISYDHIQPFITRIANGDTSPILCSSPISEFLTSSGTSGGERKLMPTIHEELDRR 123
           K+ +P++ YD ++P I RIANGD S IL S PI+EFLTSSGTS GERKLMPTI E++DRR
Sbjct: 69  KTKVPVVIYDDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRR 128

Query: 124 SLLYSLLMPVMNQFIPGLEKGKGMYFLFVKAEARTPGGLLARPVLTSYYKSSHFKDRPYD 183
            LLYSLLMPVMN ++PGL+KGK +YFLFVK E++TPGGL ARPVLTSYYKS  FK RP D
Sbjct: 129 QLLYSLLMPVMNLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPND 188

Query: 184 PYTNYTSPNEAILCTDSYQSMYAQVLCGLCHRLDVLRVGAVFASGFIRAIRFLEKHWQLL 243
           PY  YTSPNEAILC DS QSMY Q+LCGL  R +VLR+GAVFASG +RAI FL+ +W+ L
Sbjct: 189 PYNVYTSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKEL 248

Query: 244 CHDIRSGTLNSQITDQAVREAVLMSVLRRPDPELGDFIEGECCK-GSWQGIITRLWPNTK 303
             DI +GTL+S+I+D A++E+ +  +L +PD EL DFI   C +  SW+GIIT++WPNTK
Sbjct: 249 ADDISTGTLSSRISDPAIKES-MSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTK 308

Query: 304 YVDVIVTGAMSQYIGTLDYYSNGLPLVCSMYASSECYFGLNLNPLCSPNEVAYTLIPSMA 363
           Y+DVIVTGAM+QYI  L+YYS GLP+ C+MYASSE YFG+NL P+C P+EV+YT++P+MA
Sbjct: 309 YLDVIVTGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMA 368

Query: 364 YFEFLPLQTNHNHEHQLVDLTDVELGKEYELVVTTYAGLYRYRVGDILRVAGFKNNAPQF 423
           YFEFLP       + +LV+L DVE+GKEYELV+TTYAGL RYRVGDIL+V GF N+APQF
Sbjct: 369 YFEFLPHHEVPTEKSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQF 428

Query: 424 NFVCRKNVVLSIDSDKTDEVELQNAVKNSVNHLVPFDATLVDYTSYANTSTIPGHYVVYW 483
            FV RKNV+LSI+SDKTDE ELQ+AV+N+   L      +++YTSYA T TIPGHYV+YW
Sbjct: 429 KFVRRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYW 488

Query: 484 EVNQKGSTRVP-PSVFEDCCLTMEESLNSVYRQGRVSDKSIGPLEIKVVEIGTFDKLMDY 543
           E+  K  T  P   V   CCL MEESLNSVYRQ RV+DKSIGPLEI+VV+ GTF++LMDY
Sbjct: 489 ELLVKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDY 548

Query: 544 AITMGASINQYKTPRCVKFEPIVQLLNSRVVGSYFSPKCPKW 581
           AI+ GASINQYK PRCV F PI++LL+SRVV ++FSP  P W
Sbjct: 549 AISRGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALPHW 589

The following BLAST results are available for this feature:

BLAST of CmaCh04G009270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSQ4	5.4e-272	74.63	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
O81829	4.0e-267	73.81	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	3.2e-240	67.72	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	7.4e-229	65.72	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
O82333	7.4e-221	65.46	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]

BLAST of CmaCh04G009270 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1J981	0.0e+00	100.00	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita maxima OX=3661 GN=...	[more]
A0A6J1FR29	0.0e+00	99.33	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A5D3BYD3	0.0e+00	87.29	Indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo var. makuwa OX=11946...	[more]
A0A1S3BTN3	0.0e+00	87.29	indole-3-acetic acid-amido synthetase GH3.6 OS=Cucumis melo OX=3656 GN=LOC103493...	[more]
A0A6J1EW65	2.7e-309	86.28	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]

BLAST of CmaCh04G009270 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022985796.1	0.0e+00	100.00	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita maxima]	[more]
KAG6600750.1	0.0e+00	99.33	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022942727.1	0.0e+00	99.33	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_023511762.1	0.0e+00	98.99	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita pepo subsp. pepo]	[more]
XP_008452341.1	0.0e+00	87.29	PREDICTED: indole-3-acetic acid-amido synthetase GH3.6 [Cucumis melo] >KAA004700...	[more]

BLAST of CmaCh04G009270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G54510.1	3.9e-273	74.63	Auxin-responsive GH3 family protein	[more]
AT4G27260.1	2.9e-268	73.81	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	5.3e-222	65.46	Auxin-responsive GH3 family protein	[more]
AT4G37390.1	6.7e-217	62.56	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	1.3e-212	62.89	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 21..557 e-value: 9.8E-198 score: 658.3
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 7..588
None	No IPR available	PANTHER	PTHR31901:SF70	BNAC03G14570D PROTEIN	coord: 7..588

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G009270.1	CmaCh04G009270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity