CmaCh04G003340 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh04G003340
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr04: 1639073 .. 1641126 (+)
RNA-Seq Expression	CmaCh04G003340
Synteny	CmaCh04G003340

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCATCCCAATTTGCTCTTCTTCCCTATATAACTAAGCCTCTGAGAGCTACCTTAGCCCTCATCAATACCTACAACGAATTGGGTCTTTCAATGGAGAGGAGTATTCTTGGGGTGTTGTTTTTTCTTCACTTCTTTTTACCGTCTTCTTCAGCCTTCTCCAAGCATGCCTCTAATGGCATTCATTGGTGGTGCACAAAAACCACGTACCCTGAAACCTGCAAGTATTTCTATGGCAATGCCAACAATCCTCCTCCCAAAGACATGTCTGAGTTCAAGAAAATGGCGATCAAATTCGCCATGGATGGAGCTTTGAGTGTCGAGAGGCACACTAAGAGAGTCGGTTCAAGGTGTCGAAATCATAAGGAAAGGGTTGCTTGGGCTGATTGCTTGAAGCTCTACCAAAACACCATCTTTCAGCTCAAACGCACTCTAGAGTCCTCTACTAAATCTACTGAATTTGATATCCAAACCTGGCTTAGTACCGCCCTTACCAACGTTGAAACGTGCCGCACTGGGTTTGCTGAGCTGAATGTTGTGGACAATGTATTGCCGCTGATCACATCCAAGAATGTCACAAAGCTGATAAGCAATAGCTTGGCGGTTAACAATGCTTCAACTTGGCAGGGGAAAGAGACATATAAACAGGGTTTTCCAAGTTGGCTTTCAGGTGGTGATAGGAAGCTGCTGCAGTCATCTTCAATGATGGTGGACCTTGTGGTGGCACAGGATGGATCCGGTGACCATAAGACAGTTGCAGCAGCACTAGAGGAAGCTGCAAAAAGGAATACAGATGGGAGATTCGTTATACAAGTAAAGAGAGGGGTTTATGAAGAAAACCTCGAGATAGGAAATAAGATGAAGAATATAATGCTAGTCGGAGATGGAATGAGATTTACCTTTATTACAAGTAGTAGAAGTGTTGGAGGAGGCTCCACCACTTTCAATTCTGCAACCGTTGGTAAGTCTTTTTATAAACCACAACTCTCCATAATGATATGACACTTTAAGCCTAAACTCTCGTGATTGTTTATATGATTATATGATTAATCAGAGAGTTGTATTTTGTGATTTCAGCGGTGACCGGAGAAGGATTTATAGCTCAAGGCATCACATTCCGAAACACGGCCGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGGTCCGGTGCAGATCATTCCGTCTTCTACCGCTGTGCCTTTGAAGGGTATCAGGACACCCTCTATGTGCATTCACAGAGACAATTCTATAAAGAGTGCTACATTTATGGGACTGTAGATTTCATATTCGGAAACGCTGCAGTGGTTCTACAGAACTGCATGATTTACGCAAGGAAACCAATGAATGGTCAAAAGAATGCAATTACTGCTCAAGGAAGGACTGATCCAAATCAAAATACAGGCATTTCAATCCACAACTCAAGGGTCATGGCCACAGATGACCTGAAACAAGTGGAGGGCACAGTGAAAACCTTCTTGGGAAGGCCGTGGAAGGAGTACTCTCGAACAGTTTTCATTAAAACTTACCTCGATAGTTTGGTTGATCCAGCAGGTTGGTTGGAATGGAATGGTGATTTTGCATTGAACACTTTATACTATGGAGAGTATAAGAACATTGGTCCTCGTTCATCAACAGACCAGAGAGTGCAGTGGAAAGGTTACCATGTTATAAGCAATTCAACAGAGGCATCGGAGTTCTCTGTCCAAAATTTCATTTCAGGGCAGTCATGGCTGCCAGGCACAGAGGTCCCATTCATTCCTGGAATCTAGAAGAACAAAACTTTCATTCTTCCTCTGAAATAAATGTACCCAAATCATGGAATTGTTTAATCTTTATGCAGAGTTGAGAAATCATCATTCAAACTCAAATACCAAATGATAATTTACATTCATACCTCCATAATCAGCCATCAAAATTCTACTTATGTTTCTTGAACTTTCGATCTCACTTATGTTTCTTGAACTTTCAATCCTAGTTATGTTTCTCGTTCTTGACATAATATCAGAGCTTCTCGTTCTTAACAATAAGCTAAGGATTAGCACACGACAGACCAA

mRNA sequence

ATGTCATCCCAATTTGCTCTTCTTCCCTATATAACTAAGCCTCTGAGAGCTACCTTAGCCCTCATCAATACCTACAACGAATTGGGTCTTTCAATGGAGAGGAGTATTCTTGGGGTGTTGTTTTTTCTTCACTTCTTTTTACCGTCTTCTTCAGCCTTCTCCAAGCATGCCTCTAATGGCATTCATTGGTGGTGCACAAAAACCACGTACCCTGAAACCTGCAAGTATTTCTATGGCAATGCCAACAATCCTCCTCCCAAAGACATGTCTGAGTTCAAGAAAATGGCGATCAAATTCGCCATGGATGGAGCTTTGAGTGTCGAGAGGCACACTAAGAGAGTCGGTTCAAGGTGTCGAAATCATAAGGAAAGGGTTGCTTGGGCTGATTGCTTGAAGCTCTACCAAAACACCATCTTTCAGCTCAAACGCACTCTAGAGTCCTCTACTAAATCTACTGAATTTGATATCCAAACCTGGCTTAGTACCGCCCTTACCAACGTTGAAACGTGCCGCACTGGGTTTGCTGAGCTGAATGTTGTGGACAATGTATTGCCGCTGATCACATCCAAGAATGTCACAAAGCTGATAAGCAATAGCTTGGCGGTTAACAATGCTTCAACTTGGCAGGGGAAAGAGACATATAAACAGGGTTTTCCAAGTTGGCTTTCAGGTGGTGATAGGAAGCTGCTGCAGTCATCTTCAATGATGGTGGACCTTGTGGTGGCACAGGATGGATCCGGTGACCATAAGACAGTTGCAGCAGCACTAGAGGAAGCTGCAAAAAGGAATACAGATGGGAGATTCGTTATACAAGTAAAGAGAGGGGTTTATGAAGAAAACCTCGAGATAGGAAATAAGATGAAGAATATAATGCTAGTCGGAGATGGAATGAGATTTACCTTTATTACAAGTAGTAGAAGTGTTGGAGGAGGCTCCACCACTTTCAATTCTGCAACCGTTGCGGTGACCGGAGAAGGATTTATAGCTCAAGGCATCACATTCCGAAACACGGCCGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGGTCCGGTGCAGATCATTCCGTCTTCTACCGCTGTGCCTTTGAAGGGTATCAGGACACCCTCTATGTGCATTCACAGAGACAATTCTATAAAGAGTGCTACATTTATGGGACTGTAGATTTCATATTCGGAAACGCTGCAGTGGTTCTACAGAACTGCATGATTTACGCAAGGAAACCAATGAATGGTCAAAAGAATGCAATTACTGCTCAAGGAAGGACTGATCCAAATCAAAATACAGGCATTTCAATCCACAACTCAAGGGTCATGGCCACAGATGACCTGAAACAAGTGGAGGGCACAGTGAAAACCTTCTTGGGAAGGCCGTGGAAGGAGTACTCTCGAACAGTTTTCATTAAAACTTACCTCGATAGTTTGGTTGATCCAGCAGGTTGGTTGGAATGGAATGGTGATTTTGCATTGAACACTTTATACTATGGAGAGTATAAGAACATTGGTCCTCGTTCATCAACAGACCAGAGAGTGCAGTGGAAAGGTTACCATGTTATAAGCAATTCAACAGAGGCATCGGAGTTCTCTGTCCAAAATTTCATTTCAGGGCAGTCATGGCTGCCAGGCACAGAGGTCCCATTCATTCCTGGAATCTAGAAGAACAAAACTTTCATTCTTCCTCTGAAATAAATGTACCCAAATCATGGAATTGTTTAATCTTTATGCAGAGTTGAGAAATCATCATTCAAACTCAAATACCAAATGATAATTTACATTCATACCTCCATAATCAGCCATCAAAATTCTACTTATGTTTCTTGAACTTTCGATCTCACTTATGTTTCTTGAACTTTCAATCCTAGTTATGTTTCTCGTTCTTGACATAATATCAGAGCTTCTCGTTCTTAACAATAAGCTAAGGATTAGCACACGACAGACCAA

Coding sequence (CDS)

ATGTCATCCCAATTTGCTCTTCTTCCCTATATAACTAAGCCTCTGAGAGCTACCTTAGCCCTCATCAATACCTACAACGAATTGGGTCTTTCAATGGAGAGGAGTATTCTTGGGGTGTTGTTTTTTCTTCACTTCTTTTTACCGTCTTCTTCAGCCTTCTCCAAGCATGCCTCTAATGGCATTCATTGGTGGTGCACAAAAACCACGTACCCTGAAACCTGCAAGTATTTCTATGGCAATGCCAACAATCCTCCTCCCAAAGACATGTCTGAGTTCAAGAAAATGGCGATCAAATTCGCCATGGATGGAGCTTTGAGTGTCGAGAGGCACACTAAGAGAGTCGGTTCAAGGTGTCGAAATCATAAGGAAAGGGTTGCTTGGGCTGATTGCTTGAAGCTCTACCAAAACACCATCTTTCAGCTCAAACGCACTCTAGAGTCCTCTACTAAATCTACTGAATTTGATATCCAAACCTGGCTTAGTACCGCCCTTACCAACGTTGAAACGTGCCGCACTGGGTTTGCTGAGCTGAATGTTGTGGACAATGTATTGCCGCTGATCACATCCAAGAATGTCACAAAGCTGATAAGCAATAGCTTGGCGGTTAACAATGCTTCAACTTGGCAGGGGAAAGAGACATATAAACAGGGTTTTCCAAGTTGGCTTTCAGGTGGTGATAGGAAGCTGCTGCAGTCATCTTCAATGATGGTGGACCTTGTGGTGGCACAGGATGGATCCGGTGACCATAAGACAGTTGCAGCAGCACTAGAGGAAGCTGCAAAAAGGAATACAGATGGGAGATTCGTTATACAAGTAAAGAGAGGGGTTTATGAAGAAAACCTCGAGATAGGAAATAAGATGAAGAATATAATGCTAGTCGGAGATGGAATGAGATTTACCTTTATTACAAGTAGTAGAAGTGTTGGAGGAGGCTCCACCACTTTCAATTCTGCAACCGTTGCGGTGACCGGAGAAGGATTTATAGCTCAAGGCATCACATTCCGAAACACGGCCGGTCCCGAAAACCATCAAGCAGTTGCTCTCCGGTCCGGTGCAGATCATTCCGTCTTCTACCGCTGTGCCTTTGAAGGGTATCAGGACACCCTCTATGTGCATTCACAGAGACAATTCTATAAAGAGTGCTACATTTATGGGACTGTAGATTTCATATTCGGAAACGCTGCAGTGGTTCTACAGAACTGCATGATTTACGCAAGGAAACCAATGAATGGTCAAAAGAATGCAATTACTGCTCAAGGAAGGACTGATCCAAATCAAAATACAGGCATTTCAATCCACAACTCAAGGGTCATGGCCACAGATGACCTGAAACAAGTGGAGGGCACAGTGAAAACCTTCTTGGGAAGGCCGTGGAAGGAGTACTCTCGAACAGTTTTCATTAAAACTTACCTCGATAGTTTGGTTGATCCAGCAGGTTGGTTGGAATGGAATGGTGATTTTGCATTGAACACTTTATACTATGGAGAGTATAAGAACATTGGTCCTCGTTCATCAACAGACCAGAGAGTGCAGTGGAAAGGTTACCATGTTATAAGCAATTCAACAGAGGCATCGGAGTTCTCTGTCCAAAATTTCATTTCAGGGCAGTCATGGCTGCCAGGCACAGAGGTCCCATTCATTCCTGGAATCTAG

Protein sequence

MSSQFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI

Homology

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BLAST of CmaCh04G003340 vs. ExPASy Swiss-Prot
Match: O04887 (Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 6.3e-166
Identity = 293/488 (60.04%), Postives = 371/488 (76.02%), Query Frame = 0


Query: 64  WCTKTTYPETCKYFYGNANNPPP-KDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHK 123
           WC KT  P+ C+YF     +    K  ++F K++++ A++ A + +  T  +GS+CRN +
Sbjct: 31  WCGKTPNPQPCEYFLTQKTDVTSIKQDTDFYKISLQLALERATTAQSRTYTLGSKCRNER 90

Query: 124 ERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDN 183
           E+ AW DC +LY+ T+ +L +T  SS   T+ D QTWLSTALTN+ETCR    +L V + 
Sbjct: 91  EKAAWEDCRELYELTVLKLNQTSNSSPGCTKVDKQTWLSTALTNLETCRASLEDLGVPEY 150

Query: 184 VLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVA 243
           VLPL+ S NVTKLISN+L++N       + +YK GFP+W+  GDRKLLQ++    ++VVA
Sbjct: 151 VLPLL-SNNVTKLISNTLSLNKVP--YNEPSYKDGFPTWVKPGDRKLLQTTP-RANIVVA 210

Query: 244 QDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFI 303
           QDGSG+ KT+  A+  AA R    R+VI +K G Y EN+E+  K+KNIM VGDG+  T I
Sbjct: 211 QDGSGNVKTIQEAV-AAASRAGGSRYVIYIKAGTYNENIEV--KLKNIMFVGDGIGKTII 270

Query: 304 TSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAF 363
           T S+SVGGG+TTF SATVAV G+ FIA+ IT RNTAGP NHQAVALRSG+D SVFYRC+F
Sbjct: 271 TGSKSVGGGATTFKSATVAVVGDNFIARDITIRNTAGPNNHQAVALRSGSDLSVFYRCSF 330

Query: 364 EGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRT 423
           EGYQDTLYVHSQRQFY+EC IYGTVDFIFGNAAVVLQNC I+ARKP N + N +TAQGRT
Sbjct: 331 EGYQDTLYVHSQRQFYRECDIYGTVDFIFGNAAVVLQNCNIFARKPPN-RTNTLTAQGRT 390

Query: 424 DPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLE 483
           DPNQ+TGI IHN RV A  DLK V+ +VKTFLGRPWK+YSRTV+IKT+LDSL++PAGW+E
Sbjct: 391 DPNQSTGIIIHNCRVTAASDLKPVQSSVKTFLGRPWKQYSRTVYIKTFLDSLINPAGWME 450

Query: 484 WNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGT 543
           W+GDFALNTLYY EY N GP SST  RV+W+GYHV+++ ++ S+F+V NFI+G SWLP T
Sbjct: 451 WSGDFALNTLYYAEYMNTGPGSSTANRVKWRGYHVLTSPSQVSQFTVGNFIAGNSWLPAT 510

Query: 544 EVPFIPGI 551
            VPF  G+
Sbjct: 511 NVPFTSGL 510

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BLAST of CmaCh04G003340 vs. ExPASy Swiss-Prot
Match: O22149 (Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3702 GN=PME17 PE=2 SV=2)

HSP 1 Score: 566.6 bits (1459), Expect = 3.0e-160
Identity = 285/513 (55.56%), Postives = 377/513 (73.49%), Query Frame = 0


Query: 39  VLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFY-GNANNPPPKDMSEFKKMAI 98
           ++ F+   +  +S  S +    +  WC++T  P+ C+YF   N+NN P K  SEF K+++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 99  KFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQ 158
           K  +D A+  + H   +G +CR+ +E+ AW DC+KLY  T+ ++  T++ + K ++ D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 159 TWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQG 218
           TWLSTALTN++TCR GF EL V D VLPL+ S NV+ L+ N+LA+N    +      K G
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVTDIVLPLM-SNNVSNLLCNTLAINKV-PFNYTPPEKDG 187

Query: 219 FPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVY 278
           FPSW+  GDRKLLQSS+   + VVA+DGSG+ KT+  A++ A   +  GRFVI VK+GVY
Sbjct: 188 FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSGRFVIYVKQGVY 247

Query: 279 EENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNT 338
            ENLEI  + KN+ML GDG+  T IT S+SVGGG+TTFNSATVA  G+GFIA+GITFRNT
Sbjct: 248 SENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 307

Query: 339 AGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVV 398
           AG  N QAVALRSG+D SVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA V
Sbjct: 308 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 367

Query: 399 LQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRP 458
           LQNC I+AR+P   + N ITAQGR+DPNQNTGI IHNSRV A  DL+ V G+ KT+LGRP
Sbjct: 368 LQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 427

Query: 459 WKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHV 518
           W++YSRTVF+KT LDSL+DP GWLEW+G+FAL TL+Y E++N GP +ST  RV W G+ V
Sbjct: 428 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 487

Query: 519 ISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           + +++EAS+F+V  F++G SW+P + VPF  G+
Sbjct: 488 LGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

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BLAST of CmaCh04G003340 vs. ExPASy Swiss-Prot
Match: Q9FHN5 (Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3702 GN=PME59 PE=2 SV=1)

HSP 1 Score: 542.3 bits (1396), Expect = 6.1e-153
Identity = 279/533 (52.35%), Postives = 364/533 (68.29%), Query Frame = 0


Query: 40  LFFLHFFL-------PSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANN-PPPKDMSE 99
           + FLH  L       P ++   +++++    WC KT YP+ CK ++ N N    P  +SE
Sbjct: 10  ILFLHLILLVLLCVHPLTTVADRNSTD----WCDKTPYPDPCKCYFKNHNGFQQPTQLSE 69

Query: 100 FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE----- 159
           F+ M ++ AMD A+S        G  C + K++   ADC+ LY +TI QL RTL      
Sbjct: 70  FRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPK 129

Query: 160 --SSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNN 219
             ++   T+FD QTWLSTALTN ETCR G ++LNV D + P++++  ++ LISN LAVN 
Sbjct: 130 AGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNG 189

Query: 220 ASTWQGKE----TYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAA 279
           A    G +      ++GFP+WLS  D++LL+  ++  +LVVA+DGSG   TV AA++ A 
Sbjct: 190 ALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAG 249

Query: 280 KRN-TDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSAT 339
           +R  T GRFVI VKRG+Y+EN+ +     +IMLVGDGMR T IT  RSV GG TT+NSAT
Sbjct: 250 RRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSAT 309

Query: 340 VAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYK 399
             + G  FIA+GITFRNTAGP   QAVALRS +D S+FY+C+ EGYQDTL VHSQRQFY+
Sbjct: 310 AGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYR 369

Query: 400 ECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMA 459
           ECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ N ITAQGR DP QNTGISIHNSR++ 
Sbjct: 370 ECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILP 429

Query: 460 TDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEY 519
             DLK V GTVKT++GRPW ++SRTV ++TYLD++V P GW  W     F L+TL+Y EY
Sbjct: 430 APDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEY 489

Query: 520 KNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           KN GP SST  RV WKG+HV+  +++AS F+V  FI+G +WLP T +PF  G+
Sbjct: 490 KNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

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BLAST of CmaCh04G003340 vs. ExPASy Swiss-Prot
Match: Q9FHN4 (Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3702 GN=PME60 PE=2 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 1.5e-151
Identity = 281/523 (53.73%), Postives = 357/523 (68.26%), Query Frame = 0


Query: 37  LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCK-YFYGNANNPPPKDMSEFKKM 96
           L +L  L   L   +  +   S  I  WC KT YP  CK YF  ++    P  +SEF+ +
Sbjct: 14  LHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVL 73

Query: 97  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE-----SSTK 156
            ++ AMD A+S           C + K++   ADC+ LY +T+ QL RTL+     +  +
Sbjct: 74  LVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRR 133

Query: 157 STEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQG 216
            T+FD QTWLSTALTN ETCR G ++LNV D   P++++  ++ LISN LAVN A    G
Sbjct: 134 CTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAG 193

Query: 217 KETY----KQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRN-TD 276
           K        +GFP+W+S  +R+LLQ  S+  +LVVA+DGSG  KTV AA++ A +R  T 
Sbjct: 194 KNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTS 253

Query: 277 GRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGE 336
           GRFVI VKRG+Y+ENL +     NIMLVGDGMR+T IT  RSV GG TT++SAT  + G 
Sbjct: 254 GRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGL 313

Query: 337 GFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG 396
            FIA+GI F+NTAGP   QAVALRS +D S+FYRC+ EGYQDTL VHSQRQFY+ECYIYG
Sbjct: 314 HFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYG 373

Query: 397 TVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQ 456
           TVDFIFGNAAVV QNC+I  R P+ GQ N ITAQGRTD  QNTGISIHNS ++   DLK 
Sbjct: 374 TVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKP 433

Query: 457 VEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPR 516
           V  +VKT++GRPW  YSRTV +KTY+DS+V P GW  W     + L+TL+Y EYKNIGP 
Sbjct: 434 VVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPA 493

Query: 517 SSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPF 547
           SST  RV+WKG+HV+S +++AS FSV  FI+G +WLPG+ +PF
Sbjct: 494 SSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

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BLAST of CmaCh04G003340 vs. ExPASy Swiss-Prot
Match: Q9STY3 (Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3702 GN=PME33 PE=2 SV=1)

HSP 1 Score: 537.3 bits (1383), Expect = 2.0e-151
Identity = 274/514 (53.31%), Postives = 355/514 (69.07%), Query Frame = 0


Query: 58  SNGIHWWCTKTTYPETCKYFY-----GNANNPPPKDMSEFKKMAIKFAMDGALSVERHTK 117
           S  + WWC KT + ETC Y++      N N  PP+  SEF +M +K A+D A+     T 
Sbjct: 81  SGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTV 140

Query: 118 RVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE-------SSTKSTEFDIQTWLSTALT 177
           + G  C N++ + AW+DC+ L+QNT+ QL RTL+       S  K T+FD QTWLSTA T
Sbjct: 141 KFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQT 200

Query: 178 NVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKET----YKQGFPSW 237
           N+ETCR+G  +LNV D V+P+I++KN++ LI N LAVN     Q   T    +K+ FPSW
Sbjct: 201 NIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSW 260

Query: 238 LSGGDRKLLQSSSMMVD---LVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYE 297
           +S  +R+LL S+S+      LVVAQD SG  +++ AA+  AA+R    RFVI VK+GVY 
Sbjct: 261 VSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYR 320

Query: 298 ENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTA 357
           EN+++GN   NIMLVGDG R T ITS RSV  G TT+NSAT    G+ F+A+ +TF NTA
Sbjct: 321 ENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTA 380

Query: 358 GPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 417
           GP   QAVA+RS +D SVFYR    G+QDTLY+HSQRQF++ECYI GT+DFIFGNAAVV 
Sbjct: 381 GPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVF 440

Query: 418 QNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPW 477
           QNCMI  R+P++GQ N ITAQGR DP QNTGI+IH+SR++A  DLK V    KT+LGRPW
Sbjct: 441 QNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPW 500

Query: 478 KEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYH 537
           + YSR   +KTY+D+ + P GW  W    +FALNT++YGEYKN GP SST  RV+WKG+H
Sbjct: 501 QAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFH 560

Query: 538 VISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
            I+++  AS F+V + I+G SWLP T VPF  G+
Sbjct: 561 AITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

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BLAST of CmaCh04G003340 vs. ExPASy TrEMBL
Match: A0A6J1JEY1 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111486289 PE=3 SV=1)

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0


Query: 1   MSSQFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNG 60
           MSSQFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNG
Sbjct: 1   MSSQFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNG 60

Query: 61  IHWWCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRN 120
           IHWWCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRN
Sbjct: 61  IHWWCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRN 120

Query: 121 HKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVV 180
           HKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVV
Sbjct: 121 HKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVV 180

Query: 181 DNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLV 240
           DNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLV
Sbjct: 181 DNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLV 240

Query: 241 VAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFT 300
           VAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFT
Sbjct: 241 VAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFT 300

Query: 301 FITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRC 360
           FITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRC
Sbjct: 301 FITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRC 360

Query: 361 AFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQG 420
           AFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQG
Sbjct: 361 AFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQG 420

Query: 421 RTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGW 480
           RTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGW
Sbjct: 421 RTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGW 480

Query: 481 LEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLP 540
           LEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLP
Sbjct: 481 LEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLP 540

Query: 541 GTEVPFIPGI 551
           GTEVPFIPGI
Sbjct: 541 GTEVPFIPGI 550

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BLAST of CmaCh04G003340 vs. ExPASy TrEMBL
Match: A0A6J1FT96 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447927 PE=3 SV=1)

HSP 1 Score: 1021.1 bits (2639), Expect = 1.7e-294
Identity = 501/519 (96.53%), Postives = 508/519 (97.88%), Query Frame = 0


Query: 32  MERSILGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNPPPKDMSE 91
           M RS LGVLF +HFFL SSSAFSKH SNGIHWWCTKTTYPETCKYFYG+ANNPPPKDMSE
Sbjct: 1   MARSTLGVLFLIHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSE 60

Query: 92  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKS 151
           FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQL+RTL+SS KS
Sbjct: 61  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKS 120

Query: 152 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGK 211
           TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITS NVT+LISNSLAVNNASTWQGK
Sbjct: 121 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTQLISNSLAVNNASTWQGK 180

Query: 212 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQ 271
           ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKR TDGRFVIQ
Sbjct: 181 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRRTDGRFVIQ 240

Query: 272 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQG 331
           VKRGVYEENLEIGNKMKNIMLVGDGMRFTFIT SRSVGGGSTTFNSATVAVTGEGFIAQG
Sbjct: 241 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQG 300

Query: 332 ITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 391
           ITFRNTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF
Sbjct: 301 ITFRNTAGPENHQAVALRSGADRSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 360

Query: 392 GNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVK 451
           GNAAVVLQNCMIYARKPMNGQKNA+TAQGRTDPNQNTGISIHNSRVMATDDLKQVE TVK
Sbjct: 361 GNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVK 420

Query: 452 TFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ 511
           TFLGRPWKEYSRTVFIKTYLD+LVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ
Sbjct: 421 TFLGRPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ 480

Query: 512 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI
Sbjct: 481 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 519

[Close]

BLAST of CmaCh04G003340 vs. ExPASy TrEMBL
Match: A0A6J1CH86 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1)

HSP 1 Score: 907.5 bits (2344), Expect = 2.7e-260
Identity = 447/545 (82.02%), Postives = 486/545 (89.17%), Query Frame = 0


Query: 6   ALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHWWC 65
           ALLPYITKP  A LA  ++  E  L ME+S  GVLFFL FF  SS AFSKHASNGI WWC
Sbjct: 16  ALLPYITKPEVACLA--SSKQEPALLMEKS-FGVLFFLLFFSLSSPAFSKHASNGIDWWC 75

Query: 66  TKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERV 125
           +KTTYPETCKYF+ + +N  PKDMSEFKKM  KFAM+ AL  E H K VGS+CRN KE+ 
Sbjct: 76  SKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKA 135

Query: 126 AWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLP 185
           AWADCL+LYQNTI QL  TL+SS+K TEFDIQTWLSTALTN+ETC TGFAE NV D+VLP
Sbjct: 136 AWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLP 195

Query: 186 LITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDG 245
           LI S NVTKLISNSLA+NNAS W GKETYK+GFPSWL GGDR+LLQSSS+  DLVVAQDG
Sbjct: 196 LIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDG 255

Query: 246 SGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSS 305
           SGDH+TV AALE AAKR T GRFVI VKRGVYEENLEIGNKMKNIMLVGDGMRFT IT S
Sbjct: 256 SGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGS 315

Query: 306 RSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGY 365
           RSV GGSTTF+SATVAVTGEGFIA+GITFRNTAGPENHQAVALRSGAD SVFYRC FEGY
Sbjct: 316 RSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGY 375

Query: 366 QDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPN 425
           QDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR+PMNGQKNA+TAQGRTDPN
Sbjct: 376 QDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPN 435

Query: 426 QNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNG 485
           QNTGISIHNSRVMATDDLKQVE TVKT+LGRPWKEYSRTVF++TYLDSLVDPAGWLEW+G
Sbjct: 436 QNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDG 495

Query: 486 DFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVP 545
           +FALNTLYYGEYKN+GP SST QRV+W+GYH+++NSTEAS+F+VQNFI+GQSWLPGTEVP
Sbjct: 496 EFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVP 555

Query: 546 FIPGI 551
           F PG+
Sbjct: 556 FTPGL 557

[Close]

BLAST of CmaCh04G003340 vs. ExPASy TrEMBL
Match: A0A1S3AYJ9 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1)

HSP 1 Score: 893.6 bits (2308), Expect = 4.0e-256
Identity = 438/524 (83.59%), Postives = 473/524 (90.27%), Query Frame = 0


Query: 29  GLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNPPPKD 88
           GLSME S++GV  FLHFFL SSSAFSK ASNGI WWC KTTYPETCKYF+ +    PPKD
Sbjct: 3   GLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKD 62

Query: 89  MSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESS 148
           M++FKKM I+FAM+ ALS E HTK VGS+CRN KE+ AWADCLKLYQNTI QL +TL+SS
Sbjct: 63  MTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSS 122

Query: 149 TKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNAS-- 208
           TK TEFDIQTWLSTALTN+ETCRTGFAELNV+D + PLI S NVTKLISNSLA+NNAS  
Sbjct: 123 TKRTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAA 182

Query: 209 TWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDG 268
              GK TYK+GFPSWLSGGDR+LLQS+S  VDLVVAQDGSG++ TV AALEEAAKR T G
Sbjct: 183 VGNGKVTYKKGFPSWLSGGDRRLLQSASTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSG 242

Query: 269 RFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEG 328
           RFVIQVKRGVY ENLEIG+KMKNIML+GDGMRFTFIT +RSVGGGSTTFNSATVAVTGEG
Sbjct: 243 RFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEG 302

Query: 329 FIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT 388
           FIA+GITF+NTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT
Sbjct: 303 FIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT 362

Query: 389 VDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQV 448
           VDFIFGNAAVVLQNCMIYARKPMNGQKNA+TAQGRTDPNQNTGISIHNSRVMATDDLK V
Sbjct: 363 VDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPV 422

Query: 449 EGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSST 508
           E TVKT+LGRPWKEYSRTVF+KTY+DSLVDP GWLEW+GDFALNTLYYGEY NIGP S  
Sbjct: 423 ESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPI 482

Query: 509 DQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
            QRV+WKGYHVISNSTEASEF+VQNFI+GQSWLP TEVPF PG+
Sbjct: 483 SQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

[Close]

BLAST of CmaCh04G003340 vs. ExPASy TrEMBL
Match: A0A5D3DJL7 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440 PE=3 SV=1)

HSP 1 Score: 893.3 bits (2307), Expect = 5.2e-256
Identity = 438/524 (83.59%), Postives = 472/524 (90.08%), Query Frame = 0


Query: 29  GLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNPPPKD 88
           GLSME S++GV  FLHFFL SSSAFSK ASNGI WWC KTTYPETCKYF+ +    PPKD
Sbjct: 3   GLSMETSLVGVFLFLHFFLLSSSAFSKPASNGIDWWCKKTTYPETCKYFFNHGTKSPPKD 62

Query: 89  MSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESS 148
           M++FKKM I+FAM+ ALS E HTK VGS+CRN KE+ AWADCLKLYQNTI QL +TL+SS
Sbjct: 63  MTDFKKMVIQFAMERALSAESHTKGVGSKCRNGKEKAAWADCLKLYQNTILQLNQTLDSS 122

Query: 149 TKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNAS-- 208
           TK TEFDIQTWLSTALTN+ETCRTGFAELNV+D + PLI S NVTKLISNSLA+NNAS  
Sbjct: 123 TKCTEFDIQTWLSTALTNLETCRTGFAELNVLDYIWPLIMSDNVTKLISNSLAINNASAG 182

Query: 209 TWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDG 268
              GK TYK+GFPSWLSGGDR+LLQS S  VDLVVAQDGSG++ TV AALEEAAKR T G
Sbjct: 183 VGNGKVTYKKGFPSWLSGGDRRLLQSGSTKVDLVVAQDGSGNYTTVGAALEEAAKRKTSG 242

Query: 269 RFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEG 328
           RFVIQVKRGVY ENLEIG+KMKNIML+GDGMRFTFIT +RSVGGGSTTFNSATVAVTGEG
Sbjct: 243 RFVIQVKRGVYRENLEIGSKMKNIMLIGDGMRFTFITGNRSVGGGSTTFNSATVAVTGEG 302

Query: 329 FIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT 388
           FIA+GITF+NTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT
Sbjct: 303 FIARGITFQNTAGPENHQAVALRSGADLSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGT 362

Query: 389 VDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQV 448
           VDFIFGNAAVVLQNCMIYARKPMNGQKNA+TAQGRTDPNQNTGISIHNSRVMATDDLK V
Sbjct: 363 VDFIFGNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKPV 422

Query: 449 EGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSST 508
           E TVKT+LGRPWKEYSRTVF+KTY+DSLVDP GWLEW+GDFALNTLYYGEY NIGP S  
Sbjct: 423 ESTVKTYLGRPWKEYSRTVFMKTYIDSLVDPTGWLEWDGDFALNTLYYGEYNNIGPGSPI 482

Query: 509 DQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
            QRV+WKGYHVISNSTEASEF+VQNFI+GQSWLP TEVPF PG+
Sbjct: 483 SQRVKWKGYHVISNSTEASEFTVQNFIAGQSWLPNTEVPFTPGL 526

[Close]

BLAST of CmaCh04G003340 vs. NCBI nr
Match: XP_022989127.1 (pectinesterase-like [Cucurbita maxima])

HSP 1 Score: 1112.4 bits (2876), Expect = 0.0e+00
Identity = 550/550 (100.00%), Postives = 550/550 (100.00%), Query Frame = 0


Query: 1   MSSQFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNG 60
           MSSQFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNG
Sbjct: 1   MSSQFALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNG 60

Query: 61  IHWWCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRN 120
           IHWWCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRN
Sbjct: 61  IHWWCTKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRN 120

Query: 121 HKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVV 180
           HKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVV
Sbjct: 121 HKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVV 180

Query: 181 DNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLV 240
           DNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLV
Sbjct: 181 DNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLV 240

Query: 241 VAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFT 300
           VAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFT
Sbjct: 241 VAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFT 300

Query: 301 FITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRC 360
           FITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRC
Sbjct: 301 FITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRC 360

Query: 361 AFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQG 420
           AFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQG
Sbjct: 361 AFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQG 420

Query: 421 RTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGW 480
           RTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGW
Sbjct: 421 RTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGW 480

Query: 481 LEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLP 540
           LEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLP
Sbjct: 481 LEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLP 540

Query: 541 GTEVPFIPGI 551
           GTEVPFIPGI
Sbjct: 541 GTEVPFIPGI 550

[Close]

BLAST of CmaCh04G003340 vs. NCBI nr
Match: XP_023551648.1 (pectinesterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1023.1 bits (2644), Expect = 9.0e-295
Identity = 502/519 (96.72%), Postives = 509/519 (98.07%), Query Frame = 0


Query: 32  MERSILGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNPPPKDMSE 91
           M +S LGVLFFLHFFL SSSAFSKH SNGIHWWCTKTTYPETCKYFYG+ANNPPPKDMSE
Sbjct: 1   MAKSTLGVLFFLHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSE 60

Query: 92  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKS 151
           FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQL+RTL+SS KS
Sbjct: 61  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKS 120

Query: 152 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGK 211
           TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITS NVT+LISNSLAVNNASTWQGK
Sbjct: 121 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTQLISNSLAVNNASTWQGK 180

Query: 212 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQ 271
           ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKR TDGRFVIQ
Sbjct: 181 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQ 240

Query: 272 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQG 331
           VKRGVYEENLEIGNKMKNIMLVGDGMRFTFIT SRSVGGGSTTFNSATVAVTGEGFIAQG
Sbjct: 241 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQG 300

Query: 332 ITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 391
           ITFRNTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF
Sbjct: 301 ITFRNTAGPENHQAVALRSGADRSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 360

Query: 392 GNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVK 451
           GNAAVVLQNCMIYARKPMNGQKNA+TAQGRTDPNQNTGISIHNSRVMATDDLKQVE TVK
Sbjct: 361 GNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVK 420

Query: 452 TFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ 511
           TFLGRPWKEYSRTVFIKTYLD+LVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ
Sbjct: 421 TFLGRPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ 480

Query: 512 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI
Sbjct: 481 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 519

[Close]

BLAST of CmaCh04G003340 vs. NCBI nr
Match: KAG6600170.1 (Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1021.9 bits (2641), Expect = 2.0e-294
Identity = 502/519 (96.72%), Postives = 508/519 (97.88%), Query Frame = 0


Query: 32  MERSILGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNPPPKDMSE 91
           M RS LGVLFFLHFFL SSSAFSKH SNGIHWWCTKTTYPETCKYFYG+ANNPPPKDMSE
Sbjct: 1   MARSTLGVLFFLHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSE 60

Query: 92  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKS 151
           FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQL+RTL+SS KS
Sbjct: 61  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKS 120

Query: 152 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGK 211
           TE DIQTWLSTALTNVETCRTGFAELNVVDNVLPLITS NVTKLISNSLAVNNASTWQGK
Sbjct: 121 TEVDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTKLISNSLAVNNASTWQGK 180

Query: 212 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQ 271
           ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKR TDGRFVIQ
Sbjct: 181 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRKTDGRFVIQ 240

Query: 272 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQG 331
           VKRGVYEENLEIGNKMKNIMLVGDGMRFTFIT SRSVGGGSTTFNSATVAVTGEGFIAQG
Sbjct: 241 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQG 300

Query: 332 ITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 391
           ITFRNTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF
Sbjct: 301 ITFRNTAGPENHQAVALRSGADRSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 360

Query: 392 GNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVK 451
           GNAAVVLQNCMIYARKPMNGQKNA+TAQGRTDPNQNTGISIHNSRVMATDDLKQVE TVK
Sbjct: 361 GNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVK 420

Query: 452 TFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ 511
           TFLGRPWKEYSRTVFIKTYLD+LVDPAGWLEWNGDFALNT+YYGEYKNIGPRSSTDQRVQ
Sbjct: 421 TFLGRPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTVYYGEYKNIGPRSSTDQRVQ 480

Query: 512 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI
Sbjct: 481 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 519

[Close]

BLAST of CmaCh04G003340 vs. NCBI nr
Match: XP_022943089.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 1021.1 bits (2639), Expect = 3.4e-294
Identity = 501/519 (96.53%), Postives = 508/519 (97.88%), Query Frame = 0


Query: 32  MERSILGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANNPPPKDMSE 91
           M RS LGVLF +HFFL SSSAFSKH SNGIHWWCTKTTYPETCKYFYG+ANNPPPKDMSE
Sbjct: 1   MARSTLGVLFLIHFFLLSSSAFSKHVSNGIHWWCTKTTYPETCKYFYGHANNPPPKDMSE 60

Query: 92  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKS 151
           FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQL+RTL+SS KS
Sbjct: 61  FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLERTLDSSAKS 120

Query: 152 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGK 211
           TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITS NVT+LISNSLAVNNASTWQGK
Sbjct: 121 TEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSNNVTQLISNSLAVNNASTWQGK 180

Query: 212 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQ 271
           ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKR TDGRFVIQ
Sbjct: 181 ETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRRTDGRFVIQ 240

Query: 272 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQG 331
           VKRGVYEENLEIGNKMKNIMLVGDGMRFTFIT SRSVGGGSTTFNSATVAVTGEGFIAQG
Sbjct: 241 VKRGVYEENLEIGNKMKNIMLVGDGMRFTFITGSRSVGGGSTTFNSATVAVTGEGFIAQG 300

Query: 332 ITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 391
           ITFRNTAGPENHQAVALRSGAD SVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF
Sbjct: 301 ITFRNTAGPENHQAVALRSGADRSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIF 360

Query: 392 GNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVK 451
           GNAAVVLQNCMIYARKPMNGQKNA+TAQGRTDPNQNTGISIHNSRVMATDDLKQVE TVK
Sbjct: 361 GNAAVVLQNCMIYARKPMNGQKNAVTAQGRTDPNQNTGISIHNSRVMATDDLKQVESTVK 420

Query: 452 TFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ 511
           TFLGRPWKEYSRTVFIKTYLD+LVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ
Sbjct: 421 TFLGRPWKEYSRTVFIKTYLDTLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQ 480

Query: 512 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI
Sbjct: 481 WKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 519

[Close]

BLAST of CmaCh04G003340 vs. NCBI nr
Match: XP_022141175.1 (pectinesterase-like [Momordica charantia])

HSP 1 Score: 907.5 bits (2344), Expect = 5.5e-260
Identity = 447/545 (82.02%), Postives = 486/545 (89.17%), Query Frame = 0


Query: 6   ALLPYITKPLRATLALINTYNELGLSMERSILGVLFFLHFFLPSSSAFSKHASNGIHWWC 65
           ALLPYITKP  A LA  ++  E  L ME+S  GVLFFL FF  SS AFSKHASNGI WWC
Sbjct: 16  ALLPYITKPEVACLA--SSKQEPALLMEKS-FGVLFFLLFFSLSSPAFSKHASNGIDWWC 75

Query: 66  TKTTYPETCKYFYGNANNPPPKDMSEFKKMAIKFAMDGALSVERHTKRVGSRCRNHKERV 125
           +KTTYPETCKYF+ + +N  PKDMSEFKKM  KFAM+ AL  E H K VGS+CRN KE+ 
Sbjct: 76  SKTTYPETCKYFFNHGSNAAPKDMSEFKKMVTKFAMERALRAESHAKGVGSKCRNEKEKA 135

Query: 126 AWADCLKLYQNTIFQLKRTLESSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLP 185
           AWADCL+LYQNTI QL  TL+SS+K TEFDIQTWLSTALTN+ETC TGFAE NV D+VLP
Sbjct: 136 AWADCLELYQNTILQLNHTLDSSSKCTEFDIQTWLSTALTNLETCYTGFAEFNVSDHVLP 195

Query: 186 LITSKNVTKLISNSLAVNNASTWQGKETYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDG 245
           LI S NVTKLISNSLA+NNAS W GKETYK+GFPSWL GGDR+LLQSSS+  DLVVAQDG
Sbjct: 196 LIMSNNVTKLISNSLAINNASAWPGKETYKEGFPSWLLGGDRRLLQSSSVKTDLVVAQDG 255

Query: 246 SGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSS 305
           SGDH+TV AALE AAKR T GRFVI VKRGVYEENLEIGNKMKNIMLVGDGMRFT IT S
Sbjct: 256 SGDHRTVGAALEAAAKRKTGGRFVIHVKRGVYEENLEIGNKMKNIMLVGDGMRFTIITGS 315

Query: 306 RSVGGGSTTFNSATVAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGY 365
           RSV GGSTTF+SATVAVTGEGFIA+GITFRNTAGPENHQAVALRSGAD SVFYRC FEGY
Sbjct: 316 RSVRGGSTTFHSATVAVTGEGFIARGITFRNTAGPENHQAVALRSGADFSVFYRCGFEGY 375

Query: 366 QDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPN 425
           QDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYAR+PMNGQKNA+TAQGRTDPN
Sbjct: 376 QDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVLQNCMIYARRPMNGQKNAVTAQGRTDPN 435

Query: 426 QNTGISIHNSRVMATDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNG 485
           QNTGISIHNSRVMATDDLKQVE TVKT+LGRPWKEYSRTVF++TYLDSLVDPAGWLEW+G
Sbjct: 436 QNTGISIHNSRVMATDDLKQVESTVKTYLGRPWKEYSRTVFMQTYLDSLVDPAGWLEWDG 495

Query: 486 DFALNTLYYGEYKNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVP 545
           +FALNTLYYGEYKN+GP SST QRV+W+GYH+++NSTEAS+F+VQNFI+GQSWLPGTEVP
Sbjct: 496 EFALNTLYYGEYKNMGPGSSTSQRVKWRGYHIVTNSTEASKFTVQNFIAGQSWLPGTEVP 555

Query: 546 FIPGI 551
           F PG+
Sbjct: 556 FTPGL 557

[Close]

BLAST of CmaCh04G003340 vs. TAIR 10
Match: AT2G45220.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 566.6 bits (1459), Expect = 2.2e-161
Identity = 285/513 (55.56%), Postives = 377/513 (73.49%), Query Frame = 0


Query: 39  VLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCKYFY-GNANNPPPKDMSEFKKMAI 98
           ++ F+   +  +S  S +    +  WC++T  P+ C+YF   N+NN P K  SEF K+++
Sbjct: 8   IINFVILCILVASTVSGYNQKDVKAWCSQTPNPKPCEYFLTHNSNNEPIKSESEFLKISM 67

Query: 99  KFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLESSTKSTEFDIQ 158
           K  +D A+  + H   +G +CR+ +E+ AW DC+KLY  T+ ++  T++ + K ++ D Q
Sbjct: 68  KLVLDRAILAKTHAFTLGPKCRDTREKAAWEDCIKLYDLTVSKINETMDPNVKCSKLDAQ 127

Query: 159 TWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKETYKQG 218
           TWLSTALTN++TCR GF EL V D VLPL+ S NV+ L+ N+LA+N    +      K G
Sbjct: 128 TWLSTALTNLDTCRAGFLELGVTDIVLPLM-SNNVSNLLCNTLAINKV-PFNYTPPEKDG 187

Query: 219 FPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVY 278
           FPSW+  GDRKLLQSS+   + VVA+DGSG+ KT+  A++ A   +  GRFVI VK+GVY
Sbjct: 188 FPSWVKPGDRKLLQSSTPKDNAVVAKDGSGNFKTIKEAIDAA---SGSGRFVIYVKQGVY 247

Query: 279 EENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNT 338
            ENLEI  + KN+ML GDG+  T IT S+SVGGG+TTFNSATVA  G+GFIA+GITFRNT
Sbjct: 248 SENLEI--RKKNVMLRGDGIGKTIITGSKSVGGGTTTFNSATVAAVGDGFIARGITFRNT 307

Query: 339 AGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVV 398
           AG  N QAVALRSG+D SVFY+C+FE YQDTLYVHS RQFY++C +YGTVDFIFGNAA V
Sbjct: 308 AGASNEQAVALRSGSDLSVFYQCSFEAYQDTLYVHSNRQFYRDCDVYGTVDFIFGNAAAV 367

Query: 399 LQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRP 458
           LQNC I+AR+P   + N ITAQGR+DPNQNTGI IHNSRV A  DL+ V G+ KT+LGRP
Sbjct: 368 LQNCNIFARRP-RSKTNTITAQGRSDPNQNTGIIIHNSRVTAASDLRPVLGSTKTYLGRP 427

Query: 459 WKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYHV 518
           W++YSRTVF+KT LDSL+DP GWLEW+G+FAL TL+Y E++N GP +ST  RV W G+ V
Sbjct: 428 WRQYSRTVFMKTSLDSLIDPRGWLEWDGNFALKTLFYAEFQNTGPGASTSGRVTWPGFRV 487

Query: 519 ISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           + +++EAS+F+V  F++G SW+P + VPF  G+
Sbjct: 488 LGSASEASKFTVGTFLAGGSWIP-SSVPFTSGL 511

[Close]

BLAST of CmaCh04G003340 vs. TAIR 10
Match: AT5G51490.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 542.3 bits (1396), Expect = 4.4e-154
Identity = 279/533 (52.35%), Postives = 364/533 (68.29%), Query Frame = 0


Query: 40  LFFLHFFL-------PSSSAFSKHASNGIHWWCTKTTYPETCKYFYGNANN-PPPKDMSE 99
           + FLH  L       P ++   +++++    WC KT YP+ CK ++ N N    P  +SE
Sbjct: 10  ILFLHLILLVLLCVHPLTTVADRNSTD----WCDKTPYPDPCKCYFKNHNGFQQPTQLSE 69

Query: 100 FKKMAIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE----- 159
           F+ M ++ AMD A+S        G  C + K++   ADC+ LY +TI QL RTL      
Sbjct: 70  FRVMLVEAAMDRAISARAELTNSGKNCTDSKKQAVLADCIDLYGDTIMQLNRTLHGVSPK 129

Query: 160 --SSTKSTEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNN 219
             ++   T+FD QTWLSTALTN ETCR G ++LNV D + P++++  ++ LISN LAVN 
Sbjct: 130 AGAAKSCTDFDAQTWLSTALTNTETCRRGSSDLNVTDFITPIVSNTKISHLISNCLAVNG 189

Query: 220 ASTWQGKE----TYKQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAA 279
           A    G +      ++GFP+WLS  D++LL+  ++  +LVVA+DGSG   TV AA++ A 
Sbjct: 190 ALLTAGNKGNTTANQKGFPTWLSRKDKRLLR--AVRANLVVAKDGSGHFNTVQAAIDVAG 249

Query: 280 KRN-TDGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSAT 339
           +R  T GRFVI VKRG+Y+EN+ +     +IMLVGDGMR T IT  RSV GG TT+NSAT
Sbjct: 250 RRKVTSGRFVIYVKRGIYQENINVRLNNDDIMLVGDGMRSTIITGGRSVQGGYTTYNSAT 309

Query: 340 VAVTGEGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYK 399
             + G  FIA+GITFRNTAGP   QAVALRS +D S+FY+C+ EGYQDTL VHSQRQFY+
Sbjct: 310 AGIEGLHFIAKGITFRNTAGPAKGQAVALRSSSDLSIFYKCSIEGYQDTLMVHSQRQFYR 369

Query: 400 ECYIYGTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMA 459
           ECYIYGTVDFIFGNAA V QNC+I  R+P+ GQ N ITAQGR DP QNTGISIHNSR++ 
Sbjct: 370 ECYIYGTVDFIFGNAAAVFQNCLILPRRPLKGQANVITAQGRADPFQNTGISIHNSRILP 429

Query: 460 TDDLKQVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEY 519
             DLK V GTVKT++GRPW ++SRTV ++TYLD++V P GW  W     F L+TL+Y EY
Sbjct: 430 APDLKPVVGTVKTYMGRPWMKFSRTVVLQTYLDNVVSPVGWSPWIEGSVFGLDTLFYAEY 489

Query: 520 KNIGPRSSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           KN GP SST  RV WKG+HV+  +++AS F+V  FI+G +WLP T +PF  G+
Sbjct: 490 KNTGPASSTRWRVSWKGFHVLGRASDASAFTVGKFIAGTAWLPRTGIPFTSGL 536

[Close]

BLAST of CmaCh04G003340 vs. TAIR 10
Match: AT5G51500.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 537.7 bits (1384), Expect = 1.1e-152
Identity = 281/523 (53.73%), Postives = 357/523 (68.26%), Query Frame = 0


Query: 37  LGVLFFLHFFLPSSSAFSKHASNGIHWWCTKTTYPETCK-YFYGNANNPPPKDMSEFKKM 96
           L +L  L   L   +  +   S  I  WC KT YP  CK YF  ++    P  +SEF+ +
Sbjct: 14  LHLLLILLLCLRPLTTVADGNSTNIDGWCDKTPYPYPCKRYFIKHSGFRLPTQISEFRVL 73

Query: 97  AIKFAMDGALSVERHTKRVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE-----SSTK 156
            ++ AMD A+S           C + K++   ADC+ LY +T+ QL RTL+     +  +
Sbjct: 74  LVEAAMDRAVSAWDKLTNSSKNCTDFKKQAVLADCINLYGDTVMQLNRTLQGVSSKTGRR 133

Query: 157 STEFDIQTWLSTALTNVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQG 216
            T+FD QTWLSTALTN ETCR G ++LNV D   P++++  ++ LISN LAVN A    G
Sbjct: 134 CTDFDAQTWLSTALTNTETCRRGSSDLNVSDFTTPIVSNTKISHLISNCLAVNGALLTAG 193

Query: 217 KETY----KQGFPSWLSGGDRKLLQSSSMMVDLVVAQDGSGDHKTVAAALEEAAKRN-TD 276
           K        +GFP+W+S  +R+LLQ  S+  +LVVA+DGSG  KTV AA++ A +R  T 
Sbjct: 194 KNDSTTGDSKGFPTWVSRKERRLLQLQSVRANLVVAKDGSGHFKTVQAAIDVAGRRKVTS 253

Query: 277 GRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGE 336
           GRFVI VKRG+Y+ENL +     NIMLVGDGMR+T IT  RSV GG TT++SAT  + G 
Sbjct: 254 GRFVIYVKRGIYQENLNVRLNNDNIMLVGDGMRYTIITGGRSVKGGYTTYSSATAGIEGL 313

Query: 337 GFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYG 396
            FIA+GI F+NTAGP   QAVALRS +D S+FYRC+ EGYQDTL VHSQRQFY+ECYIYG
Sbjct: 314 HFIAKGIAFQNTAGPAKGQAVALRSSSDLSIFYRCSIEGYQDTLMVHSQRQFYRECYIYG 373

Query: 397 TVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQ 456
           TVDFIFGNAAVV QNC+I  R P+ GQ N ITAQGRTD  QNTGISIHNS ++   DLK 
Sbjct: 374 TVDFIFGNAAVVFQNCIILPRLPLKGQANVITAQGRTDLFQNTGISIHNSIIIPAPDLKP 433

Query: 457 VEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPR 516
           V  +VKT++GRPW  YSRTV +KTY+DS+V P GW  W     + L+TL+Y EYKNIGP 
Sbjct: 434 VVRSVKTYMGRPWMMYSRTVVLKTYIDSVVSPVGWSPWTKGSTYGLDTLFYAEYKNIGPA 493

Query: 517 SSTDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPF 547
           SST  RV+WKG+HV+S +++AS FSV  FI+G +WLPG+ +PF
Sbjct: 494 SSTRWRVRWKGFHVLSKASDASAFSVGKFIAGTAWLPGSGIPF 536

[Close]

BLAST of CmaCh04G003340 vs. TAIR 10
Match: AT3G47400.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 537.3 bits (1383), Expect = 1.4e-152
Identity = 274/514 (53.31%), Postives = 355/514 (69.07%), Query Frame = 0


Query: 58  SNGIHWWCTKTTYPETCKYFY-----GNANNPPPKDMSEFKKMAIKFAMDGALSVERHTK 117
           S  + WWC KT + ETC Y++      N N  PP+  SEF +M +K A+D A+     T 
Sbjct: 81  SGDMTWWCNKTPHAETCNYYFRKSSQNNINLRPPRFRSEFLRMLVKVALDQAVITHSQTV 140

Query: 118 RVGSRCRNHKERVAWADCLKLYQNTIFQLKRTLE-------SSTKSTEFDIQTWLSTALT 177
           + G  C N++ + AW+DC+ L+QNT+ QL RTL+       S  K T+FD QTWLSTA T
Sbjct: 141 KFGPSCTNNQRKAAWSDCVNLFQNTVAQLNRTLKGLNPAASSDVKCTDFDAQTWLSTAQT 200

Query: 178 NVETCRTGFAELNVVDNVLPLITSKNVTKLISNSLAVNNASTWQGKET----YKQGFPSW 237
           N+ETCR+G  +LNV D V+P+I++KN++ LI N LAVN     Q   T    +K+ FPSW
Sbjct: 201 NIETCRSGSEDLNVSDFVMPVISNKNLSDLIGNCLAVNGVLMKQHDHTTTANHKEYFPSW 260

Query: 238 LSGGDRKLLQSSSMMVD---LVVAQDGSGDHKTVAAALEEAAKRNTDGRFVIQVKRGVYE 297
           +S  +R+LL S+S+      LVVAQD SG  +++ AA+  AA+R    RFVI VK+GVY 
Sbjct: 261 VSRHERRLLVSASLAKSSPHLVVAQDRSGHFRSIQAAINFAARRRFKSRFVIYVKKGVYR 320

Query: 298 ENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTGEGFIAQGITFRNTA 357
           EN+++GN   NIMLVGDG R T ITS RSV  G TT+NSAT    G+ F+A+ +TF NTA
Sbjct: 321 ENIDVGNDNHNIMLVGDGERKTIITSGRSVQHGYTTYNSATGGFGGQRFVAKDMTFINTA 380

Query: 358 GPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIYGTVDFIFGNAAVVL 417
           GP   QAVA+RS +D SVFYR    G+QDTLY+HSQRQF++ECYI GT+DFIFGNAAVV 
Sbjct: 381 GPLRGQAVAVRSSSDLSVFYRVGIHGFQDTLYIHSQRQFFRECYISGTIDFIFGNAAVVF 440

Query: 418 QNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLKQVEGTVKTFLGRPW 477
           QNCMI  R+P++GQ N ITAQGR DP QNTGI+IH+SR++A  DLK V    KT+LGRPW
Sbjct: 441 QNCMILVRRPLHGQANIITAQGRGDPFQNTGITIHSSRIIAASDLKPVIRAYKTYLGRPW 500

Query: 478 KEYSRTVFIKTYLDSLVDPAGWLEW--NGDFALNTLYYGEYKNIGPRSSTDQRVQWKGYH 537
           + YSR   +KTY+D+ + P GW  W    +FALNT++YGEYKN GP SST  RV+WKG+H
Sbjct: 501 QAYSRVTIMKTYIDNSISPLGWSPWLRGSNFALNTVFYGEYKNFGPGSSTRWRVRWKGFH 560

Query: 538 VISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
            I+++  AS F+V + I+G SWLP T VPF  G+
Sbjct: 561 AITSTAVASRFTVGSLIAGGSWLPATGVPFKSGL 594

[Close]

BLAST of CmaCh04G003340 vs. TAIR 10
Match: AT1G23200.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 430.6 bits (1106), Expect = 1.8e-120
Identity = 243/526 (46.20%), Postives = 315/526 (59.89%), Query Frame = 0


Query: 65  CTKTTYPETCKYFYGNANNPPPKDMSE---FKKMAIKFAMDGALSVERHTKRVGSRCRNH 124
           C +T YP  C +   N+      D ++   F  + +   MD A+ + R    +      H
Sbjct: 38  CKQTPYPSVCDHHMSNSPLKTLDDQTDGFTFHDLVVSSTMDQAVQLHRLVSSLKQHHSLH 97

Query: 125 KERV-AWADCLKLYQNTIFQLKRTLES-STKSTEFDIQTWLSTALTNVETCRTGFAELNV 184
           K    A  DCL+LY++TI QL  +  S    S+  D QT LS A+ N +TCR GF +  +
Sbjct: 98  KHATSALFDCLELYEDTIDQLNHSRRSYGQYSSPHDRQTSLSAAIANQDTCRNGFRDFKL 157

Query: 185 VDN---VLPLITSKNVTKLISNSLAVNNAS---------------TWQGKETYKQG---- 244
             +     P+   +N+TK ISNSLAV  A+               T   K+    G    
Sbjct: 158 TSSYSKYFPVQFHRNLTKSISNSLAVTKAAAEAEAVAEKYPSTGFTKFSKQRSSAGGGSH 217

Query: 245 ----------FPSWLSGGDRKLLQSS--SMMVDLVVAQDGSGDHKTVAAALEEAAK-RNT 304
                     FPSW    DRKLL+ S  +   DLVVA+DGSG + ++  A+  AAK    
Sbjct: 218 RRLLLFSDEKFPSWFPLSDRKLLEDSKTTAKADLVVAKDGSGHYTSIQQAVNAAAKLPRR 277

Query: 305 DGRFVIQVKRGVYEENLEIGNKMKNIMLVGDGMRFTFITSSRSVGGGSTTFNSATVAVTG 364
           + R VI VK GVY EN+ I   +KN+M++GDG+  T +T +R+V  G+TTF SAT AV+G
Sbjct: 278 NQRLVIYVKAGVYRENVVIKKSIKNVMVIGDGIDSTIVTGNRNVQDGTTTFRSATFAVSG 337

Query: 365 EGFIAQGITFRNTAGPENHQAVALRSGADHSVFYRCAFEGYQDTLYVHSQRQFYKECYIY 424
            GFIAQGITF NTAGPE HQAVALRS +D SVFY C+F+GYQDTLY+HS RQF + C IY
Sbjct: 338 NGFIAQGITFENTAGPEKHQAVALRSSSDFSVFYACSFKGYQDTLYLHSSRQFLRNCNIY 397

Query: 425 GTVDFIFGNAAVVLQNCMIYARKPMNGQKNAITAQGRTDPNQNTGISIHNSRVMATDDLK 484
           GTVDFIFG+A  +LQNC IYARKPM+GQKN ITAQ R +P++ TG  I +S V    +  
Sbjct: 398 GTVDFIFGDATAILQNCNIYARKPMSGQKNTITAQSRKEPDETTGFVIQSSTVATASE-- 457

Query: 485 QVEGTVKTFLGRPWKEYSRTVFIKTYLDSLVDPAGWLEWNGDFALNTLYYGEYKNIGPRS 544
                  T+LGRPW+ +SRTVF+K  L +LV PAGWL W+G FAL+TLYYGEY N G  +
Sbjct: 458 -------TYLGRPWRSHSRTVFMKCNLGALVSPAGWLPWSGSFALSTLYYGEYGNTGAGA 517

Query: 545 STDQRVQWKGYHVISNSTEASEFSVQNFISGQSWLPGTEVPFIPGI 551
           S   RV+W GYHVI   TEA +F+V+NF+ G  W+  T VP   G+
Sbjct: 518 SVSGRVKWPGYHVIKTVTEAEKFTVENFLDGNYWITATGVPVNDGL 554

The following BLAST results are available for this feature:

BLAST of CmaCh04G003340 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O04887	6.3e-166	60.04	Pectinesterase 2 OS=Citrus sinensis OX=2711 GN=PECS-2.1 PE=2 SV=1	[more]
O22149	3.0e-160	55.56	Probable pectinesterase/pectinesterase inhibitor 17 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN5	6.1e-153	52.35	Probable pectinesterase/pectinesterase inhibitor 59 OS=Arabidopsis thaliana OX=3...	[more]
Q9FHN4	1.5e-151	53.73	Probable pectinesterase/pectinesterase inhibitor 60 OS=Arabidopsis thaliana OX=3...	[more]
Q9STY3	2.0e-151	53.31	Probable pectinesterase/pectinesterase inhibitor 33 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmaCh04G003340 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JEY1	0.0e+00	100.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111486289 PE=3 SV=1	[more]
A0A6J1FT96	1.7e-294	96.53	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111447927 PE=3 SV=1	[more]
A0A6J1CH86	2.7e-260	82.02	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111011631 PE=3 SV=1	[more]
A0A1S3AYJ9	4.0e-256	83.59	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103483986 PE=3 SV=1	[more]
A0A5D3DJL7	5.2e-256	83.59	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold577G00440...	[more]

BLAST of CmaCh04G003340 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022989127.1	0.0e+00	100.00	pectinesterase-like [Cucurbita maxima]	[more]
XP_023551648.1	9.0e-295	96.72	pectinesterase-like [Cucurbita pepo subsp. pepo]	[more]
KAG6600170.1	2.0e-294	96.72	Pectinesterase 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022943089.1	3.4e-294	96.53	pectinesterase-like [Cucurbita moschata]	[more]
XP_022141175.1	5.5e-260	82.02	pectinesterase-like [Momordica charantia]	[more]

BLAST of CmaCh04G003340 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G45220.1	2.2e-161	55.56	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51490.1	4.4e-154	52.35	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G51500.1	1.1e-152	53.73	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G47400.1	1.4e-152	53.31	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT1G23200.1	1.8e-120	46.20	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 56..202 e-value: 9.1E-31 score: 118.2
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 35..204 e-value: 2.6E-25 score: 86.8
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 61..201 e-value: 8.5E-25 score: 87.8
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 238..535 e-value: 2.8E-143 score: 476.1
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 56..205 e-value: 1.3E-29 score: 105.2
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 59..204
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 232..550 e-value: 1.1E-123 score: 414.7
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 38..549
None	No IPR available	PANTHER	PTHR31707:SF181	PECTINESTERASE	coord: 38..549
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 63..205 e-value: 3.53935E-34 score: 124.858
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 383..392
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 238..549

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh04G003340.1	CmaCh04G003340.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity