CmaCh03G012000 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G012000 vs. ExPASy TrEMBL
Match: A0A6J1IVJ0 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita maxima OX=3661 GN=LOC111478776 PE=4 SV=1)

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60
           MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD
Sbjct: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60

Query: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120
           YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK
Sbjct: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120

Query: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180
           PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA
Sbjct: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180

Query: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240
           FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM
Sbjct: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240

Query: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300
           ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF
Sbjct: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300

Query: 301 PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360
           PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG
Sbjct: 301 PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360

Query: 361 QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420
           QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG
Sbjct: 361 QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420

Query: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480
           HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI
Sbjct: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480

Query: 481 FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540
           FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL
Sbjct: 481 FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540

Query: 541 VLRTWEFYKGDIYKKFREERQETE 565
           VLRTWEFYKGDIYKKFREERQETE
Sbjct: 541 VLRTWEFYKGDIYKKFREERQETE 564

BLAST of CmaCh03G012000 vs. ExPASy TrEMBL
Match: A0A6J1ECZ3 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Cucurbita moschata OX=3662 GN=LOC111433047 PE=4 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.2e-303
Identity = 546/564 (96.81%), Postives = 554/564 (98.23%), Query Frame = 0

Query: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60
           MGGVRFSIRT RSSVPMRNFV QVLLGRWFTVFASLLIMSVSGATYM ALYSADIKSSLD
Sbjct: 1   MGGVRFSIRTLRSSVPMRNFVWQVLLGRWFTVFASLLIMSVSGATYMFALYSADIKSSLD 60

Query: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120
           YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK
Sbjct: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120

Query: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180
           PKLPAMCL ICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKG+VGLSGAILTQIYHA
Sbjct: 121 PKLPAMCLYICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHA 180

Query: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240
           FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEV+RIS S+DLKNFYYMLYTSLVLAGFLM
Sbjct: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVNRISKSSDLKNFYYMLYTSLVLAGFLM 240

Query: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300
           IL+IIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMD+ISV PLDP 
Sbjct: 241 ILIIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDVISVLPLDPS 300

Query: 301 PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360
           PVELSSSRPP SCFKNVF+PPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG
Sbjct: 301 PVELSSSRPPSSCFKNVFKPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360

Query: 361 QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420
           QIGSSLGYSAHSISTFTSL+SIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG
Sbjct: 361 QIGSSLGYSAHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420

Query: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480
           HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI
Sbjct: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480

Query: 481 FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540
           FNVKVAGFLYDQEARKQMAASGL +VAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL
Sbjct: 481 FNVKVAGFLYDQEARKQMAASGLVTVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540

Query: 541 VLRTWEFYKGDIYKKFREERQETE 565
           VLRTWEFYKGDIYKKFREER ETE
Sbjct: 541 VLRTWEFYKGDIYKKFREERTETE 564

BLAST of CmaCh03G012000 vs. ExPASy TrEMBL
Match: A0A6J1CR80 (protein NUCLEAR FUSION DEFECTIVE 4-like OS=Momordica charantia OX=3673 GN=LOC111013589 PE=4 SV=1)

HSP 1 Score: 903.3 bits (2333), Expect = 5.2e-259
Identity = 475/576 (82.47%), Postives = 510/576 (88.54%), Query Frame = 0

Query: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60
           MGG R  I +      MRNF LQVLLGRWFTVFASLLIM+VSGATYM  LYSADIKSSL 
Sbjct: 1   MGGARLRIWSGSGRRQMRNFALQVLLGRWFTVFASLLIMAVSGATYMFGLYSADIKSSLG 60

Query: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120
           YDQTTLNL+GFFKDLGANVGV SGLINEITPPWVVL IGGVMNFFGYFMIWLSVT RI K
Sbjct: 61  YDQTTLNLLGFFKDLGANVGVLSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTQRIAK 120

Query: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180
           PK+PAMCL ICLGANSQT+ANTGALIPSVKNFP+ RGNVLGLLKG+VGLSGAILTQ+YHA
Sbjct: 121 PKVPAMCLYICLGANSQTYANTGALIPSVKNFPEYRGNVLGLLKGFVGLSGAILTQLYHA 180

Query: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240
           FYGDDSKDFILLIAWLPTAVSFLLLR+VRVVEVD+ S SNDLK FY++LY SL LA FLM
Sbjct: 181 FYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVDQRSKSNDLKIFYHLLYISLGLACFLM 240

Query: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300
           IL+IIQNEL+FTRIQYLGCA ILLVLLFLPL VVI EEF ++KRK Q +++ S    DP 
Sbjct: 241 ILIIIQNELVFTRIQYLGCALILLVLLFLPLAVVITEEFSIKKRKLQALNVTSWLS-DPS 300

Query: 301 PVELSSSRP------------PISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICG 360
           P EL SSRP            P SCF+NVFRPPERG+DYTILQAIFS+DMLILFVATICG
Sbjct: 301 PAELPSSRPPHLPTAVVAVANPSSCFENVFRPPERGEDYTILQAIFSIDMLILFVATICG 360

Query: 361 AGGALTAMDNLGQIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPL 420
            GG LTA+DNLGQIGSSLGYS HSISTFTSL+SIWGFLGRAFSGYASEFLWTKY+FSRPL
Sbjct: 361 TGGTLTAIDNLGQIGSSLGYSTHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPL 420

Query: 421 FFTLVLLFSCVGHLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYS 480
           F TLVLL SC+GHLLIAFGVPTSLYF+SVIIGFCFGAQWPLIFAIVSELFGLKYYATLYS
Sbjct: 421 FLTLVLLLSCIGHLLIAFGVPTSLYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYS 480

Query: 481 ISGIASPVGSYIFNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITA 540
           ISGIASPVG+YIF+VKVAG+LYD+EAR+QMAA GL  VAG DLACKGV CYRLAFLII+A
Sbjct: 481 ISGIASPVGTYIFSVKVAGYLYDEEARRQMAAIGLRRVAG-DLACKGVQCYRLAFLIISA 540

Query: 541 ATVLGCVVSFILVLRTWEFYKGDIYKKFREERQETE 565
           ATV GC+VSFILVLRTWEFYKGDIYKKFREE +E E
Sbjct: 541 ATVFGCIVSFILVLRTWEFYKGDIYKKFREETKEAE 574

BLAST of CmaCh03G012000 vs. ExPASy TrEMBL
Match: A0A5A7V9Y5 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold827G00110 PE=4 SV=1)

HSP 1 Score: 901.0 bits (2327), Expect = 2.6e-258
Identity = 474/575 (82.43%), Postives = 510/575 (88.70%), Query Frame = 0

Query: 5   RFSIRT-SRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLDYDQ 64
           RFSI T  R    M NF +QVLLGRWFT FASLLIMSVSGA+YM ALYS+DIKSSL+YDQ
Sbjct: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72

Query: 65  TTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPKPKL 124
           TTLNL+GFFKDLG+NVGVFSGLINEITPPWVVL IGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132

Query: 125 PAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHAFYG 184
           PAMCL I LGANSQTFANTGALIPSVKNFPQNRGNVLGLLKG+VGLSGAILTQIYHAFYG
Sbjct: 133 PAMCLFIFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192

Query: 185 DDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLMILV 244
           DDSKDFILLIAWLPTAVS LLLR+VRVVEV+ I  SNDLKNFY MLY SL LAGFLMIL+
Sbjct: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252

Query: 245 IIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLIS-VP-PLDPFP 304
           IIQNEL+FTRIQYL C F+LL  LFLPLVVVIREEF +RKRK Q +D+ S +P P D  P
Sbjct: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312

Query: 305 VELSSSRP------------PISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGA 364
            EL   RP            P SCF+NVFRPPERG+DYTILQAIFSVDMLILF  TICGA
Sbjct: 313 AELPLPRPSSLRMTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372

Query: 365 GGALTAMDNLGQIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLF 424
           GG LTA+DNLGQIGSSLGYS H+ISTFTSL+SIWGFLGRAFSGYASEFLWTKY+FSRPLF
Sbjct: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432

Query: 425 FTLVLLFSCVGHLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 484
            T VLLFSC GHLLIA GVPTS+YF+SVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI
Sbjct: 433 LTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492

Query: 485 SGIASPVGSYIFNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAA 544
           SGIASP+GSYIFNVKVAG+LYDQEARKQ+ A GL +VAGRDLACKGVHCYRLAFLII+A+
Sbjct: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISAS 552

Query: 545 TVLGCVVSFILVLRTWEFYKGDIYKKFREERQETE 565
           T+ GC VSFILVLRTW+FYKGDIYKKFR+ER+E++
Sbjct: 553 TMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 587

BLAST of CmaCh03G012000 vs. ExPASy TrEMBL
Match: A0A5D3C849 (Protein NUCLEAR FUSION DEFECTIVE 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold202G00430 PE=4 SV=1)

HSP 1 Score: 900.6 bits (2326), Expect = 3.4e-258
Identity = 473/575 (82.26%), Postives = 510/575 (88.70%), Query Frame = 0

Query: 5   RFSIRT-SRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLDYDQ 64
           RFSI T  R    M NF +QVLLGRWFT FASLLIMSVSGA+YM ALYS+DIKSSL+YDQ
Sbjct: 13  RFSIGTGQRRRSQMSNFAVQVLLGRWFTAFASLLIMSVSGASYMFALYSSDIKSSLNYDQ 72

Query: 65  TTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPKPKL 124
           TTLNL+GFFKDLG+NVGVFSGLINEITPPWVVL IGGVMNFFGYFMIWLSVTHRI KPKL
Sbjct: 73  TTLNLVGFFKDLGSNVGVFSGLINEITPPWVVLFIGGVMNFFGYFMIWLSVTHRISKPKL 132

Query: 125 PAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHAFYG 184
           PAMCL + LGANSQTFANTGALIPSVKNFPQNRGNVLGLLKG+VGLSGAILTQIYHAFYG
Sbjct: 133 PAMCLFVFLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHAFYG 192

Query: 185 DDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLMILV 244
           DDSKDFILLIAWLPTAVS LLLR+VRVVEV+ I  SNDLKNFY MLY SL LAGFLMIL+
Sbjct: 193 DDSKDFILLIAWLPTAVSLLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFLMILI 252

Query: 245 IIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLIS-VP-PLDPFP 304
           IIQNEL+FTRIQYL C F+LL  LFLPLVVVIREEF +RKRK Q +D+ S +P P D  P
Sbjct: 253 IIQNELMFTRIQYLVCVFVLLAFLFLPLVVVIREEFSIRKRKLQGVDVTSWLPVPSDQSP 312

Query: 305 VELSSSRP------------PISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGA 364
            EL   RP            P SCF+NVFRPPERG+DYTILQAIFSVDMLILF  TICGA
Sbjct: 313 AELPLPRPSSLRTTDIALTNPSSCFENVFRPPERGEDYTILQAIFSVDMLILFFTTICGA 372

Query: 365 GGALTAMDNLGQIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLF 424
           GG LTA+DNLGQIGSSLGYS H+ISTFTSL+SIWGFLGRAFSGYASEFLWTKY+FSRPLF
Sbjct: 373 GGTLTAIDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLF 432

Query: 425 FTLVLLFSCVGHLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 484
            T VLLFSC GHLLIA GVPTS+YF+SVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI
Sbjct: 433 LTFVLLFSCFGHLLIASGVPTSIYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSI 492

Query: 485 SGIASPVGSYIFNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAA 544
           SGIASP+GSYIFNVKVAG+LYDQEARKQ+ A GL +VAGRDLACKGVHCYRLAFLII+A+
Sbjct: 493 SGIASPIGSYIFNVKVAGYLYDQEARKQIGAIGLRNVAGRDLACKGVHCYRLAFLIISAS 552

Query: 545 TVLGCVVSFILVLRTWEFYKGDIYKKFREERQETE 565
           T+ GC VSFILVLRTW+FYKGDIYKKFR+ER+E++
Sbjct: 553 TMFGCFVSFILVLRTWKFYKGDIYKKFRDERKESK 587

BLAST of CmaCh03G012000 vs. NCBI nr
Match: XP_022978994.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita maxima])

HSP 1 Score: 1088.6 bits (2814), Expect = 0.0e+00
Identity = 564/564 (100.00%), Postives = 564/564 (100.00%), Query Frame = 0

Query: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60
           MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD
Sbjct: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60

Query: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120
           YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK
Sbjct: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120

Query: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180
           PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA
Sbjct: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180

Query: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240
           FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM
Sbjct: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240

Query: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300
           ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF
Sbjct: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300

Query: 301 PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360
           PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG
Sbjct: 301 PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360

Query: 361 QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420
           QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG
Sbjct: 361 QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420

Query: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480
           HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI
Sbjct: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480

Query: 481 FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540
           FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL
Sbjct: 481 FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540

Query: 541 VLRTWEFYKGDIYKKFREERQETE 565
           VLRTWEFYKGDIYKKFREERQETE
Sbjct: 541 VLRTWEFYKGDIYKKFREERQETE 564

BLAST of CmaCh03G012000 vs. NCBI nr
Match: KAG6604391.1 (Protein NUCLEAR FUSION DEFECTIVE 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1054.7 bits (2726), Expect = 2.9e-304
Identity = 547/564 (96.99%), Postives = 556/564 (98.58%), Query Frame = 0

Query: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60
           MGGVRFSIRT RSSVPMRNFVLQVLLGRWFTVFASL+IMSVSGATYM ALYSADIKSSLD
Sbjct: 46  MGGVRFSIRTLRSSVPMRNFVLQVLLGRWFTVFASLVIMSVSGATYMFALYSADIKSSLD 105

Query: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120
           YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK
Sbjct: 106 YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 165

Query: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180
           PKLPAMCL ICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKG+VGLSGAILTQIYHA
Sbjct: 166 PKLPAMCLYICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHA 225

Query: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240
           FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEV+RIS S+DLKNFYYMLYTSLVLAGFLM
Sbjct: 226 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVNRISKSSDLKNFYYMLYTSLVLAGFLM 285

Query: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300
           IL+IIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMD+ISV PLDP 
Sbjct: 286 ILIIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDVISVLPLDPS 345

Query: 301 PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360
           PVELSSSRPP SCFKNVF+PPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG
Sbjct: 346 PVELSSSRPPSSCFKNVFKPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 405

Query: 361 QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420
           QIGSSLGYSAHSISTFTSL+SIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG
Sbjct: 406 QIGSSLGYSAHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 465

Query: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480
           HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI
Sbjct: 466 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 525

Query: 481 FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540
           FNVKVAGFLYDQEARKQMAASGL SVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL
Sbjct: 526 FNVKVAGFLYDQEARKQMAASGLVSVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 585

Query: 541 VLRTWEFYKGDIYKKFREERQETE 565
           VLRTWEFYKGDIYKKFREER+ETE
Sbjct: 586 VLRTWEFYKGDIYKKFREERKETE 609

BLAST of CmaCh03G012000 vs. NCBI nr
Match: XP_023544231.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1053.1 bits (2722), Expect = 8.4e-304
Identity = 547/564 (96.99%), Postives = 554/564 (98.23%), Query Frame = 0

Query: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60
           MGGVRFSIRT RSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYM ALYSADIKSSLD
Sbjct: 1   MGGVRFSIRTLRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMFALYSADIKSSLD 60

Query: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120
           YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVT RIPK
Sbjct: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTQRIPK 120

Query: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180
           PKLPAMCL ICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKG+VGLSGAILTQIYHA
Sbjct: 121 PKLPAMCLYICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHA 180

Query: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240
           FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEV+RIS SNDLKNFYYMLYTSLVLAGFLM
Sbjct: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVNRISKSNDLKNFYYMLYTSLVLAGFLM 240

Query: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300
           IL+IIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEF+MRKRKSQDMD+ISV PLDP 
Sbjct: 241 ILIIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFIMRKRKSQDMDVISVLPLDPS 300

Query: 301 PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360
           PVELSSSRPP SCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG
Sbjct: 301 PVELSSSRPPSSCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360

Query: 361 QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420
           QIGSSLGYSAHSISTFTSL+SIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG
Sbjct: 361 QIGSSLGYSAHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420

Query: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480
           HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI
Sbjct: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480

Query: 481 FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540
           FNVKVAGFLYDQEARKQMAASGL SVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL
Sbjct: 481 FNVKVAGFLYDQEARKQMAASGLVSVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540

Query: 541 VLRTWEFYKGDIYKKFREERQETE 565
           VLRTWEFYKGDIYKKFREER+E E
Sbjct: 541 VLRTWEFYKGDIYKKFREERKEAE 564

BLAST of CmaCh03G012000 vs. NCBI nr
Match: XP_022925716.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Cucurbita moschata])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.4e-303
Identity = 546/564 (96.81%), Postives = 554/564 (98.23%), Query Frame = 0

Query: 1   MGGVRFSIRTSRSSVPMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLD 60
           MGGVRFSIRT RSSVPMRNFV QVLLGRWFTVFASLLIMSVSGATYM ALYSADIKSSLD
Sbjct: 1   MGGVRFSIRTLRSSVPMRNFVWQVLLGRWFTVFASLLIMSVSGATYMFALYSADIKSSLD 60

Query: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120
           YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK
Sbjct: 61  YDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPK 120

Query: 121 PKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHA 180
           PKLPAMCL ICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKG+VGLSGAILTQIYHA
Sbjct: 121 PKLPAMCLYICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYHA 180

Query: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLM 240
           FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEV+RIS S+DLKNFYYMLYTSLVLAGFLM
Sbjct: 181 FYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVNRISKSSDLKNFYYMLYTSLVLAGFLM 240

Query: 241 ILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPF 300
           IL+IIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMD+ISV PLDP 
Sbjct: 241 ILIIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDVISVLPLDPS 300

Query: 301 PVELSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360
           PVELSSSRPP SCFKNVF+PPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG
Sbjct: 301 PVELSSSRPPSSCFKNVFKPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLG 360

Query: 361 QIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420
           QIGSSLGYSAHSISTFTSL+SIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG
Sbjct: 361 QIGSSLGYSAHSISTFTSLVSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVG 420

Query: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480
           HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI
Sbjct: 421 HLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYI 480

Query: 481 FNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540
           FNVKVAGFLYDQEARKQMAASGL +VAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL
Sbjct: 481 FNVKVAGFLYDQEARKQMAASGLVTVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFIL 540

Query: 541 VLRTWEFYKGDIYKKFREERQETE 565
           VLRTWEFYKGDIYKKFREER ETE
Sbjct: 541 VLRTWEFYKGDIYKKFREERTETE 564

BLAST of CmaCh03G012000 vs. NCBI nr
Match: XP_038882767.1 (protein NUCLEAR FUSION DEFECTIVE 4-like [Benincasa hispida])

HSP 1 Score: 948.7 bits (2451), Expect = 2.2e-272
Identity = 500/571 (87.57%), Postives = 523/571 (91.59%), Query Frame = 0

Query: 1   MGGVRFSIRTSRSSV-PMRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSL 60
           M   RFSIRT R  + PM NFV+QVLLGRWFTVFASLLIMSVSGATYM ALYSADIKSSL
Sbjct: 1   MKSARFSIRTGRRRLSPMTNFVVQVLLGRWFTVFASLLIMSVSGATYMFALYSADIKSSL 60

Query: 61  DYDQTTLNLIGFFKDLGANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIP 120
           +YDQTTLNLIGFFKDLG+NVGVFSGLI EITPPWVVL IGGVMNFFGYFMIWLSVTHRIP
Sbjct: 61  NYDQTTLNLIGFFKDLGSNVGVFSGLITEITPPWVVLFIGGVMNFFGYFMIWLSVTHRIP 120

Query: 121 KPKLPAMCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYH 180
           KPKLPAMCL ICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKG+VGLSGAILTQIYH
Sbjct: 121 KPKLPAMCLFICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGFVGLSGAILTQIYH 180

Query: 181 AFYGDDSKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFL 240
           AFYGDDSKDFILLIAWLPTAVSFLLLR+VRVVEV+ I  SNDLKNFY MLY SL LAGFL
Sbjct: 181 AFYGDDSKDFILLIAWLPTAVSFLLLRIVRVVEVNPIFKSNDLKNFYSMLYISLGLAGFL 240

Query: 241 MILVIIQNELIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLIS---VPP 300
           MIL+IIQNEL+FTRIQYLGCAF+LL  LFLPLVVVIREEF  RKRK Q +DL S   VPP
Sbjct: 241 MILIIIQNELMFTRIQYLGCAFVLLAFLFLPLVVVIREEFSSRKRKLQGVDLTSWLPVPP 300

Query: 301 LDPFPVELSSSRP---PISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGAL 360
            DP P EL   RP     SCF+NVFRPPERG+DYTILQAIFSVDMLILFVATICGAGG L
Sbjct: 301 -DPSPAELPLPRPSNSSSSCFENVFRPPERGEDYTILQAIFSVDMLILFVATICGAGGTL 360

Query: 361 TAMDNLGQIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLV 420
           TAMDNLGQIGSSLGYS H+ISTFTSL+SIWGFLGRAFSGYASEFLWTKY+FSRPLF TL+
Sbjct: 361 TAMDNLGQIGSSLGYSTHTISTFTSLVSIWGFLGRAFSGYASEFLWTKYNFSRPLFLTLL 420

Query: 421 LLFSCVGHLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIA 480
           LL SCVGHLLI+FGVPTSLYF+SVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIA
Sbjct: 421 LLLSCVGHLLISFGVPTSLYFASVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIA 480

Query: 481 SPVGSYIFNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLG 540
           SPVGSYIFNVKVAG+LYDQEARKQMAA GL SVAGRDLAC GVHCYRLAFLII+AATV G
Sbjct: 481 SPVGSYIFNVKVAGYLYDQEARKQMAAIGLKSVAGRDLACVGVHCYRLAFLIISAATVFG 540

Query: 541 CVVSFILVLRTWEFYKGDIYKKFREERQETE 565
           CVVSFILVLRTW+FYKGDIYKKFREER+E E
Sbjct: 541 CVVSFILVLRTWKFYKGDIYKKFREERKEAE 570

BLAST of CmaCh03G012000 vs. TAIR 10
Match: AT2G39210.1 (Major facilitator superfamily protein )

HSP 1 Score: 645.2 bits (1663), Expect = 4.9e-185
Identity = 333/571 (58.32%), Postives = 419/571 (73.38%), Query Frame = 0

Query: 17  MRNFVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLDYDQTTLNLIGFFKDLG 76
           M++  +Q+L GRWF  F SLLIMS +GATYM  +YS DIK +L YDQTTLNL+ FFKDLG
Sbjct: 10  MKSLTIQILTGRWFMFFGSLLIMSTAGATYMFGIYSGDIKETLGYDQTTLNLLSFFKDLG 69

Query: 77  ANVGVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPKPKLPAMCLCICLGANS 136
           ANVGV +GL+NE+TPPW +LLIG ++NFFGYFMIWL+VT RI KP++  MCL IC+GANS
Sbjct: 70  ANVGVLAGLLNEVTPPWFILLIGAILNFFGYFMIWLAVTERISKPQVWHMCLYICVGANS 129

Query: 137 QTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHAFYGDDSKDFILLIAWL 196
           Q+FANTG+L+  VKNFP++RG VLG+LKGYVGLSGAI+TQ+Y AFYG+D+K+ IL+I WL
Sbjct: 130 QSFANTGSLVTCVKNFPESRGVVLGILKGYVGLSGAIITQLYRAFYGEDTKELILMIGWL 189

Query: 197 PTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLMILVIIQNELIFTRIQY 256
           P  VSF  LR +R+++V R   +N+LK FY  LY SL LA FLM+++II     FT+ ++
Sbjct: 190 PAIVSFAFLRTIRIMKVKR--QTNELKVFYNFLYISLGLATFLMVVIIINKLSGFTQSEF 249

Query: 257 LGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPFPVELSSSRPPI----- 316
            G A +++VLL LP++VVI EE  + K K   ++       DP P+ + + +P +     
Sbjct: 250 GGSAAVVIVLLLLPIIVVILEEKKLWKEKQVALN-------DPAPINVVTEKPKLDSSEF 309

Query: 317 ------------------SCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGAL 376
                             SC+  VF PPERGDDYTILQA+FSVDMLILF+ATICG GG L
Sbjct: 310 KDDDGEESKEVVEKVKTPSCWTTVFNPPERGDDYTILQALFSVDMLILFLATICGVGGTL 369

Query: 377 TAMDNLGQIGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLV 436
           TA+DNLGQIG+SLGY   S+STF SL+SIW + GR  SG  SE    KY F RPL  T+V
Sbjct: 370 TAIDNLGQIGNSLGYPKRSVSTFVSLVSIWNYYGRVVSGVVSEIFLIKYKFPRPLMLTMV 429

Query: 437 LLFSCVGHLLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIA 496
           LL SC GHLLIAF VP  LY +SVIIGFCFGAQWPL+FAI+SE+FGLKYY+TLY+   +A
Sbjct: 430 LLLSCAGHLLIAFNVPGGLYVASVIIGFCFGAQWPLLFAIISEIFGLKYYSTLYNFGSVA 489

Query: 497 SPVGSYIFNVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLG 556
           SP+GSY+ NV+VAG+LYD EA KQ  A G   V G+DL C G  C++L+F+II A T+ G
Sbjct: 490 SPIGSYLLNVRVAGYLYDVEAGKQYKALGKTRVEGQDLNCIGTSCFKLSFIIIAAVTLFG 549

Query: 557 CVVSFILVLRTWEFYKGDIYKKFREERQETE 565
            +VS +LV+RT +FYK DIYKKFRE+    E
Sbjct: 550 VLVSMVLVIRTKKFYKSDIYKKFREKALAAE 571

BLAST of CmaCh03G012000 vs. TAIR 10
Match: AT2G28120.1 (Major facilitator superfamily protein )

HSP 1 Score: 588.2 bits (1515), Expect = 7.1e-168
Identity = 313/557 (56.19%), Postives = 391/557 (70.20%), Query Frame = 0

Query: 20  FVLQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLDYDQTTLNLIGFFKDLGANV 79
           F++    GRWF VFAS LIM+ +GATY+   YS DIKS+L YDQTTLNL+GFFKDLGANV
Sbjct: 9   FLIHFFNGRWFMVFASFLIMACAGATYLFGTYSKDIKSTLGYDQTTLNLLGFFKDLGANV 68

Query: 80  GVFSGLINEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPKPKLPAMCLCICLGANSQTF 139
           GV SGLI E+TP W VL IG  MNF GYFMIWL+VT ++ KPK+  MCL IC+GANSQ F
Sbjct: 69  GVLSGLIAEVTPTWFVLTIGSAMNFVGYFMIWLTVTGKVAKPKVWQMCLYICIGANSQNF 128

Query: 140 ANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHAFYGDDSKDFILLIAWLPTA 199
           ANTGAL+  VKNFP++RG +LGLLKGYVGLSGAI TQ+Y A YG DSK  ILLIAWLP A
Sbjct: 129 ANTGALVTCVKNFPESRGVMLGLLKGYVGLSGAIFTQLYFAIYGHDSKSLILLIAWLPAA 188

Query: 200 VSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLMILVIIQNELIFTRIQYLGC 259
           VS + + ++R  +V  +   N+L  FY  LY S+ LA FLM + I + ++ F++  Y   
Sbjct: 189 VSLVFVYLIREKKV--VRQRNELSVFYQFLYISIFLALFLMAMNIAEKQVHFSKAAYAAS 248

Query: 260 AFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPFPVELSSSRPPI-------- 319
           A I   LLF+PL V +++E        +  +++ +P  +P  V++   +  +        
Sbjct: 249 ATICCALLFVPLTVSVKQEL-------EVWNMMKLPIEEPSEVKVEKPKKELDLDQDKAA 308

Query: 320 ----------SCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLGQ 379
                     SCF  VF PP RG+DYTILQA+ S DM+ILFVAT CG G +LTA+DNLGQ
Sbjct: 309 KVNGEEKETKSCFSTVFSPPPRGEDYTILQALLSTDMIILFVATFCGLGSSLTAVDNLGQ 368

Query: 380 IGSSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVGH 439
           IG SLGY  H++S+F SL+SIW + GR FSG+ SE+L  KY   RPL  TLVLL SC GH
Sbjct: 369 IGESLGYPNHTVSSFVSLVSIWNYFGRVFSGFVSEYLLAKYKLPRPLMMTLVLLLSCAGH 428

Query: 440 LLIAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYIF 499
           LLIAF VP S+Y +S+++GF FGAQ PL+FAI+SELFGLKYY+TL++   +ASP+GSYI 
Sbjct: 429 LLIAFPVPGSVYIASILMGFSFGAQLPLLFAIISELFGLKYYSTLFNCGQLASPLGSYIL 488

Query: 500 NVKVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFILV 559
           NV+V G LYD+EA KQ+ A GL     +DL C G  CY+L FLI+ A T  G +VS  L 
Sbjct: 489 NVRVTGMLYDKEALKQLTARGLTRKDVKDLTCLGSQCYKLPFLILAAVTFFGALVSLGLA 548

BLAST of CmaCh03G012000 vs. TAIR 10
Match: AT1G74780.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 345.1 bits (884), Expect = 1.0e-94
Identity = 210/552 (38.04%), Postives = 315/552 (57.07%), Query Frame = 0

Query: 22  LQVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLDYDQTTLNLIGFFKDLGANVGV 81
           +++L  +W  + AS+ I   SGA+Y   +YSA +KS+  YDQ+TL+ +  FKD+GAN GV
Sbjct: 1   MEILRTKWVAMTASIWIQCASGASYTFGIYSAVLKSTQSYDQSTLDTVSVFKDIGANAGV 60

Query: 82  FSGL----------------INEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPKPKLPA 141
           FSGL                I     PWVVL +G +  F GYF+IW SVT  I KP +P 
Sbjct: 61  FSGLLYTYATSNRLRGRGGGIGGAGGPWVVLAVGAIQCFAGYFLIWASVTGLIRKPPVPL 120

Query: 142 MCLCICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHAFYGDD 201
           MCL + L A SQTF NT  ++ +V+NF    G  +G++KG++GLSGAIL Q+Y      D
Sbjct: 121 MCLFMFLAAQSQTFFNTANVVSAVENFADYGGTAVGIMKGFLGLSGAILIQLYETLCAGD 180

Query: 202 SKDFILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLMILVII 261
              FILL+A  PT +S L++ +VR+ E    S+++D K+   +   SL++A +LMI++I+
Sbjct: 181 PASFILLLAVTPTVLSLLVMPLVRIYET---SVADDKKHLNGLSAVSLIIAAYLMIIIIL 240

Query: 262 QNELIFTRIQYLGCAFILLVLLFLPLVVVIR-EEFLMRKRKSQDMD-LISVPPLDPFPVE 321
           +N    +    +     LLV+L LPL++  R +   M K    D   LIS P       +
Sbjct: 241 KNTFGLSSWANIVTLVCLLVMLALPLLIARRAQRDGMEKTVPHDYSPLISSPKATTSGNQ 300

Query: 322 LSSSRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLGQIG 381
            S     +             ++  +LQA+  +   +LF+A ICG G  L+ ++N+ QIG
Sbjct: 301 SSEGDSKVEA--------GLSENLNLLQAMKKLSFWLLFLAMICGMGSGLSTINNIRQIG 360

Query: 382 SSLGYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVGHLL 441
            SL YS+  I++  SL SIW FLGR  +GYAS+ L  K  + RPL     L    +GHL+
Sbjct: 361 ESLRYSSVEINSLVSLWSIWNFLGRFGAGYASDALLHKKGWPRPLLMAATLGTMSIGHLI 420

Query: 442 IAFGVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYIFNV 501
           IA G   +LY  SVI+G C+G+QW L+  I SELFG+++  T+++   +ASP+GSYIF+V
Sbjct: 421 IASGFQGNLYVGSVIVGVCYGSQWSLMPTITSELFGIRHMGTIFNTISVASPIGSYIFSV 480

Query: 502 KVAGFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFILVLR 556
           ++ G++YD+ A            +G    C G HC+RL+F+I+ +    G +V+ +L  R
Sbjct: 481 RLIGYIYDKTA------------SGEGNTCYGSHCFRLSFIIMASVAFFGFLVAIVLFFR 529

BLAST of CmaCh03G012000 vs. TAIR 10
Match: AT1G18940.1 (Nodulin-like / Major Facilitator Superfamily protein )

HSP 1 Score: 334.7 bits (857), Expect = 1.4e-91
Identity = 205/554 (37.00%), Postives = 319/554 (57.58%), Query Frame = 0

Query: 23  QVLLGRWFTVFASLLIMSVSGATYMLALYSADIKSSLDYDQTTLNLIGFFKDLGANVGVF 82
           ++L  +W  + AS+ I   +G +Y   +YSA +KS+  YDQ+TL+ +  FKD+G NVGV 
Sbjct: 4   EILRTKWMAMTASIWIQCSAGGSYTFGIYSAILKSTQSYDQSTLDTVSVFKDIGGNVGVL 63

Query: 83  SGLINEITP--------------PWVVLLIGGVMNFFGYFMIWLSVTHRIPKPKLPAMCL 142
           SGL+                   PWVV+LIG ++NF GYF++W SVT  I +P +P MCL
Sbjct: 64  SGLVYTAATFNRRRRDGRERRGGPWVVILIGAILNFTGYFLMWASVTGLIKRPPVPVMCL 123

Query: 143 CICLGANSQTFANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHAFYGDDSKD 202
            + + A S TF NT  ++ S++NF    G  +G++KG+VGLSGA+L Q+Y      D K 
Sbjct: 124 FMFIAAQSLTFLNTANVVSSLENFADYGGTAVGIMKGFVGLSGAMLIQLYEVVCPGDPKT 183

Query: 203 FILLIAWLPTAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLMILVIIQNE 262
           FILL+A +P+ +S L++ +VRV +   +   ++ K+   +   SL++A +LMI +I+++ 
Sbjct: 184 FILLLAIVPSLLSVLVMPLVRVYKTSTV---DEKKHLDGLSTLSLIIAAYLMITIILKST 243

Query: 263 LIFTRIQYLGCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISV--PPLDPFPVELSS 322
           L            +LLVLL  PL+V +R       R S +  L SV  P +D      S 
Sbjct: 244 LSLPSWANAVTLAVLLVLLSSPLLVAVRAH-----RDSIEKPLSSVYSPLVDNLEATTSG 303

Query: 323 SRPPISCFKNVFRPPERGDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLGQIGSSL 382
               +   K++           +LQA+ +VD  +LF+A ICG G  ++ ++N+ QIG SL
Sbjct: 304 EILMLDEDKSL----------NLLQAMCNVDFWLLFLAMICGMGSGISTINNIRQIGESL 363

Query: 383 GYSAHSISTFTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVGHLLIAF 442
            Y++  I++  +L +IW F+GR   GY S++L  +  + RPL     L    +GHL+IA 
Sbjct: 364 RYTSVEINSLLALWNIWNFIGRFGGGYVSDWLLHRKGWPRPLLMATTLGTMTIGHLIIAS 423

Query: 443 GVPTSLYFSSVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYIFNVKVA 502
           G   +LY  S+I+G C+G+QW L+  I SELFG+K+  T+Y+   IASP+GSYIF+V++ 
Sbjct: 424 GFQGNLYPGSIIVGICYGSQWSLMPTITSELFGVKHMGTIYNTISIASPMGSYIFSVRLI 483

Query: 503 GFLYDQEARKQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFILVLRTWE 561
           G++YD+            ++ G    C G HC+RLA+++I +   LG +VS +LV RT  
Sbjct: 484 GYIYDR------------TIIGEGNTCYGPHCFRLAYVVIASVAFLGFLVSCVLVFRTKT 526

BLAST of CmaCh03G012000 vs. TAIR 10
Match: AT2G34355.1 (Major facilitator superfamily protein )

HSP 1 Score: 334.0 bits (855), Expect = 2.4e-91
Identity = 202/540 (37.41%), Postives = 310/540 (57.41%), Query Frame = 0

Query: 28  RWFTVFASLLIMSVSGATYMLALYSADIKSSLDYDQTTLNLIGFFKDLGANVGVFSGLI- 87
           +W    AS+ I S SGATY  A+YS+ +KSS  YDQ+TL+ +  FKD+G   G+ SG + 
Sbjct: 7   KWVAAAASIWIQSFSGATYTFAIYSSILKSSQSYDQSTLDFVSVFKDIGGTFGIISGFLY 66

Query: 88  -------NEITPPWVVLLIGGVMNFFGYFMIWLSVTHRIPKPKLPAMCLCICLGANSQTF 147
                       PWVV+ +G V  F G+F IW SV   I  P +P MCL + L  +S  F
Sbjct: 67  TAMTSKSRGFGGPWVVVFVGLVQWFVGFFFIWASVVGLIAPPPVPLMCLFVFLAGHSLPF 126

Query: 148 ANTGALIPSVKNFPQNRGNVLGLLKGYVGLSGAILTQIYHAFYGDDSK--DFILLIAWLP 207
            NT  ++ + +NF Q  G  +G+++G++GLSGAIL Q+YHA  G +     FILL+A +P
Sbjct: 127 FNTANVVTAARNFSQYGGTAVGIMQGFLGLSGAILIQLYHAVCGGEGNPATFILLLAIVP 186

Query: 208 TAVSFLLLRVVRVVEVDRISISNDLKNFYYMLYTSLVLAGFLMILVIIQNELIFTRIQYL 267
           T V FL +  VRV E   IS   D K+   +   S+++A +LM+++ ++N L  +R   +
Sbjct: 187 TLVMFLAMPFVRVYETVTIS---DKKHLDGLSAISMIIAAYLMVVITVENVLGLSRSMQI 246

Query: 268 GCAFILLVLLFLPLVVVIREEFLMRKRKSQDMDLISVPPLDPFPVELSSSRPPISCFKNV 327
               ++L+LL  PL+V +R      + K Q +  +  P LD   +      PP S   N+
Sbjct: 247 FSFILVLLLLASPLLVAVR----ALREKRQTLSSLDGPVLDTSAL----LDPPSS---NI 306

Query: 328 FRPPER--GDDYTILQAIFSVDMLILFVATICGAGGALTAMDNLGQIGSSLGYSAHSIST 387
           F   +    +D  IL+A+ +V+  +LF+A +CG G     ++N+ QIG SL YS+  +++
Sbjct: 307 FPDGDHLVAEDSNILEAMSTVNFWLLFLAMLCGMGSGFATVNNMRQIGESLRYSSVQLNS 366

Query: 388 FTSLMSIWGFLGRAFSGYASEFLWTKYSFSRPLFFTLVLLFSCVGHLLIAFGVPTSLYFS 447
             SL SIW FLGR  +GY S+    K+S+ RP+F  + L    +GH+++A GV  SLY  
Sbjct: 367 LVSLWSIWNFLGRFGAGYVSDTFLHKHSWPRPIFMAITLGVMAIGHIIVASGVQGSLYAG 426

Query: 448 SVIIGFCFGAQWPLIFAIVSELFGLKYYATLYSISGIASPVGSYIFNVKVAGFLYDQEAR 507
           SV+IG  +G+QW L+  I SE+FG+++  T+Y    IA P+GSYI +VKV G+ YD+ A 
Sbjct: 427 SVLIGMAYGSQWSLMPTITSEIFGIRHMGTIYFTISIAGPIGSYILSVKVIGYFYDKVAS 486

Query: 508 KQMAASGLASVAGRDLACKGVHCYRLAFLIITAATVLGCVVSFILVLRTWEFYKGDIYKK 556
           +             D +C G  C+R +F+I+ +  + G +V+ +L  RT +FYK  + K+
Sbjct: 487 ED------------DNSCFGSQCFRTSFMIMASVALFGSLVASVLFFRTHKFYKNLVAKR 520