CmaCh03G004020 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G004020 vs. ExPASy Swiss-Prot
Match: Q3E8X3 (Protein NRT1/ PTR FAMILY 2.8 OS=Arabidopsis thaliana OX=3702 GN=NPF2.8 PE=2 SV=2)

HSP 1 Score: 536.6 bits (1381), Expect = 3.4e-151
Identity = 278/533 (52.16%), Postives = 366/533 (68.67%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           + ++  NE+FEKLASMSLI NL +YL T YNL  V    V+ IW G+ N   LAGAF++D
Sbjct: 24  IKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNILTLAGAFVSD 83

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 146
           AYLGRF+TLL GS AS +GMG+  L A  P +RP  C    +   QP  WQLG L+ GLG
Sbjct: 84  AYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQLGVLFSGLG 143

Query: 147 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 206
           LL IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F NWWY  F+VALVIALT VVY+QT+
Sbjct: 144 LLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFNWWYFSFTVALVIALTGVVYIQTN 203

Query: 207 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 266
           ISW +GF IPTAC   SI  F++G+  YIC   +GSVFAD+ KV+ A C+KR V   + I
Sbjct: 204 ISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKVVTAACKKRKVKPGSDI 263

Query: 267 DHLYDPPIDSSK---LAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLK 326
                P  D S    +    R   FDKA+ V  +  EL+E G +K +W+LCSV QV+ LK
Sbjct: 264 TFYIGPSNDGSPTTLVRDKHRLRFFDKASIV-TNPNELNEDGNAKYKWRLCSVQQVKNLK 323

Query: 327 CVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPH-FQIPPAWMSLTPMIALSIW 386
           CV  I+P+W  GI CFI   Q   +GILQA+QM++  GPH FQ+P  WM+L  MI L+IW
Sbjct: 324 CVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQVPAGWMNLVSMITLAIW 383

Query: 387 IYIYE-KYVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFV 446
           I +YE   +  +K+ T + KR ++K RIE  IVM ++CM+VAG  E  RR+SA++N  FV
Sbjct: 384 ISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGFQEKKRRASALKNGSFV 443

Query: 447 SPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVL 506
           SP+ +  L+P+FAL+GLTEAF+A+A+ME LT  +PE +R VAGAIFFLS S+ SY+ ++L
Sbjct: 444 SPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGAIFFLSSSIASYICTLL 503

Query: 507 MAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHF 555
           + ++  + R EG    WLG  DLNKNRL+ +F+++  I   N  YFR FA+ +
Sbjct: 504 INVIDAVTRKEG--KSWLGDKDLNKNRLENYFFIIAGIQVANLLYFRLFASRY 551

BLAST of CmaCh03G004020 vs. ExPASy Swiss-Prot
Match: Q9LFX9 (Protein NRT1/ PTR FAMILY 2.12 OS=Arabidopsis thaliana OX=3702 GN=NPF2.12 PE=1 SV=2)

HSP 1 Score: 405.2 bits (1040), Expect = 1.2e-111
Identity = 216/537 (40.22%), Postives = 343/537 (63.87%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           + F+ GNET EKL S+ + AN +LYL  +++++ V++  V  +W G TNFA L GA ++D
Sbjct: 21  ITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLGALISD 80

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGE-SNCPQPHPWQLGFLYLGL 146
           AY+GRF T+ + S  S+LG+  +TL A  P++ PPPC +     C  P+  QLG L+LGL
Sbjct: 81  AYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGILFLGL 140

Query: 147 GLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQT 206
           G L IG+GGIRPC+I FG DQFD  TE+G   + SF NW+YL  ++ L+ + T+VVYLQT
Sbjct: 141 GFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVVVYLQT 200

Query: 207 SISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQ 266
            +SW +GF+IPT+    ++++F +G RFY+   P+GSVF+ +A+V+VA  +KR++ K + 
Sbjct: 201 -VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL-KISL 260

Query: 267 ID----HLYDPPIDS---SKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQ 326
           +D      Y+PP+     SKL  TD+F   DKAA + D   +L  +G   N+W+LCS+ +
Sbjct: 261 VDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLCSIQE 320

Query: 327 VEQLKCVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIA 386
           VE++KC++ +VP+W+AGI   ++M    +F + QA +M+R +GPHF+IP A +++   I 
Sbjct: 321 VEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVISYIT 380

Query: 387 LSIWIYIYEK-YVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVEN 446
           + IW+ IYE   V F+ +   +  R ++  R+ IGIV ++L M  AG +E VRR+ A E 
Sbjct: 381 IGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTRATE- 440

Query: 447 KLFVSPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYM 506
              ++ + V+ L     L GL E+F  I ++E   +  PE +R++A ++F LS +  +Y+
Sbjct: 441 ---MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAAANYL 500

Query: 507 SSVLMAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHF 555
           SS+L+  V K++  +    +WL   DL++ +LDYF+Y++ V+   N  YF + A  +
Sbjct: 501 SSLLVTTVHKVSGTK-DHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCAHRY 545

BLAST of CmaCh03G004020 vs. ExPASy Swiss-Prot
Match: Q8RX77 (Protein NRT1/ PTR FAMILY 2.13 OS=Arabidopsis thaliana OX=3702 GN=NPF2.13 PE=1 SV=1)

HSP 1 Score: 404.8 bits (1039), Expect = 1.6e-111
Identity = 217/537 (40.41%), Postives = 342/537 (63.69%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           V F+ GNET E+L S+ L+AN ++YL  +++L+ VD+A V+ IWSG TN   L GA+++D
Sbjct: 58  VSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVGAYISD 117

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGES-NCPQPHPWQLGFLYLGL 146
            Y+GRF T+ F SFA+LLG+  +TL A FP++ P  C   +  +C  P+  Q+G L LGL
Sbjct: 118 TYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGL 177

Query: 147 GLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQT 206
             L +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y+ F+V L+I  T+VVY+Q 
Sbjct: 178 CFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVVYIQD 237

Query: 207 SISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQ 266
            +SW +GF+IPT     ++++F  G + Y+   P+GS+F+ +A+V+VA  +KR +    +
Sbjct: 238 QVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAE 297

Query: 267 ID---HLYDPPIDS---SKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQV 326
            D     YDP I S   SKL  +++F   DKAA V +   +L  +G   ++W+LCSV +V
Sbjct: 298 DDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPEGPPADKWRLCSVQEV 357

Query: 327 EQLKCVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIAL 386
           E++KC++ IVPIW+AGI    +M   G+F + QA++M+R +GP F+IP   +S+  ++ +
Sbjct: 358 EEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTI 417

Query: 387 SIWIYIYEK-YVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVE-- 446
            I++  Y++ +V FM++ T      ++  RI  GIV ++  M+VAGI+E +RR  ++   
Sbjct: 418 GIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAG 477

Query: 447 NKLFVSPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSY 506
           +   ++P+ V+ L P+  L GL EAF  I  +E   +  PE +R++A ++F LS +  SY
Sbjct: 478 DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSY 537

Query: 507 MSSVLMAIVSKINRVEGG--KSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFA 552
           +SS L+ +V K +   GG  + +WL  N LN  +LDYF+Y++ V+   N  YF + A
Sbjct: 538 LSSFLVTVVHKFS---GGHDRPDWLNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYCA 588

BLAST of CmaCh03G004020 vs. ExPASy Swiss-Prot
Match: Q9LV10 (Protein NRT1/ PTR FAMILY 2.11 OS=Arabidopsis thaliana OX=3702 GN=NPF2.11 PE=1 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 6.3e-97
Identity = 202/534 (37.83%), Postives = 313/534 (58.61%), Query Frame = 0

Query: 29  FVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLADAY 88
           F+ GNETFEKL  +  ++NL++YL  ++NL S+ +A ++  +SGT NF     AFL D Y
Sbjct: 52  FIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFGTFVAAFLCDTY 111

Query: 89  LGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPC-RHGESNCPQPHPWQLGFLYLGLGL 148
            GR+ TL     A  LG  V+ L A  P++ P  C    +S C  P   Q+ FL +GLG 
Sbjct: 112 FGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFLLMGLGF 171

Query: 149 LVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSI 208
           LV+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++  F+ A +++LTLVVY+Q+++
Sbjct: 172 LVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVVYVQSNV 231

Query: 209 SWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQ-- 268
           SWT+G  IP      + LIF  G + Y+     GS  A +A+V+    +KR +    Q  
Sbjct: 232 SWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLKPAKQPW 291

Query: 269 --IDHLYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLK 328
             + + Y P   +SKL +TD+F   DKAA +     +L   GK  + WKLC++ QVE++K
Sbjct: 292 LNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADPWKLCTMQQVEEVK 351

Query: 329 CVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGP-HFQIPPAWMSLTPMIALSIW 388
           C+V ++PIW A    ++++ Q  ++ + QA+Q +R +G   F IP A   +  M  ++++
Sbjct: 352 CIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAATYVVFLMTGMTVF 411

Query: 389 IYIYEK-YVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAV------ 448
           I +Y++  V  M++ T  +   ++  RI  GI  +   +VVAG +E  RR+ A+      
Sbjct: 412 IVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEERRRTFALTKPTLG 471

Query: 449 --ENKLFVSPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSL 508
               K  +S +    LIP+ +L+G+ EAFAAI  ME      PE++R+ AG+IF++   +
Sbjct: 472 MAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRSFAGSIFYVGGGV 531

Query: 509 GSYMSSVLMAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYF 548
            SY+ S L+A V +  +   G   WL   DLNK RLD F++++  I A NF YF
Sbjct: 532 SSYLGSFLIATVHRTTQNSSG-GNWL-AEDLNKGRLDLFYFMIAGILAVNFAYF 582

BLAST of CmaCh03G004020 vs. ExPASy Swiss-Prot
Match: Q9CAR9 (Putative protein NRT1/ PTR FAMILY 2.14 OS=Arabidopsis thaliana OX=3702 GN=NPF2.14 PE=5 SV=1)

HSP 1 Score: 349.0 bits (894), Expect = 1.0e-94
Identity = 194/533 (36.40%), Postives = 324/533 (60.79%), Query Frame = 0

Query: 29  FVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLADAY 88
           ++ GNET E+LA+  L+AN ++Y+   Y++D V +  ++  WS  TNFA + GAF++D+Y
Sbjct: 27  YIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINTWSALTNFAPIIGAFISDSY 86

Query: 89  LGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGE--SNCPQPHPWQLGFLYLGLG 148
            G+F T++FGS A LLGM VLT  +  P +RPPPC   +    C      QL  L  GL 
Sbjct: 87  TGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQITGQCIPYSYSQLYVLLSGLF 146

Query: 149 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 208
           LL +G GGIR C++ F  DQFD +TE+GR    SF +W+Y   ++  ++++TLV+Y+Q +
Sbjct: 147 LLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYTTHTIVQLVSMTLVLYVQNN 206

Query: 209 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 268
           ISW +GFAIPT    F++L+  +G R+Y+   P+GSVF+ + KVLVA  +KR     + I
Sbjct: 207 ISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGVFKVLVAAYKKRKARFTSGI 266

Query: 269 DH---LYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLK 328
           D+   L +  + S+KL  TD+F   +KA  V ++    DE G    EW+ C+V Q+E +K
Sbjct: 267 DYHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNN----DEAG--NEEWRTCTVRQIEDIK 326

Query: 329 CVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMN-RFIGPHFQIPPAWMSLTPMIALSIW 388
            ++ I+PI+A+ I  F++M Q  +F + QA++M+ +F G  + IPPA +++  ++ + IW
Sbjct: 327 SIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTSYLIPPASITVISLLNIGIW 386

Query: 389 IYIYEK-YVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFV 448
           +  YE   V  ++  T +N   S+  ++ IG + S+  M+++GI+E  RR  ++      
Sbjct: 387 LPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLISGIVERKRRDLSLNG---- 446

Query: 449 SPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVL 508
             + V+ L P+  L G  + F  + + E     +P ++R++  ++ +L LSL SY+SS +
Sbjct: 447 VKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSIGNSLLYLGLSLASYLSSAM 506

Query: 509 MAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHF 555
           ++IV  +    GG+S WL  +D++K++LD F+Y +  ++  NF +F + A  +
Sbjct: 507 VSIVHSVT-ARGGQS-WL-TDDIDKSKLDCFYYFIAALSTLNFIFFFWCARRY 546

BLAST of CmaCh03G004020 vs. ExPASy TrEMBL
Match: A0A6J1IS88 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita maxima OX=3661 GN=LOC111478233 PE=3 SV=1)

HSP 1 Score: 914.4 bits (2362), Expect = 2.2e-262
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0

Query: 106 MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 165
           MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 166 QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 225
           QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 120

Query: 226 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 285
           IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF
Sbjct: 121 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 180

Query: 286 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 345
           LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM
Sbjct: 181 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 240

Query: 346 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 405
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 300

Query: 406 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 465
           KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 360

Query: 466 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 525
           AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 420

Query: 526 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV
Sbjct: 421 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 455

BLAST of CmaCh03G004020 vs. ExPASy TrEMBL
Match: A0A6J1GFB3 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111453646 PE=3 SV=1)

HSP 1 Score: 887.5 bits (2292), Expect = 2.9e-254
Identity = 442/456 (96.93%), Postives = 449/456 (98.46%), Query Frame = 0

Query: 106 MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 165
           MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 166 QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 225
           QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVY+QTSISWTLGFAIPTACFVFSIL
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYVQTSISWTLGFAIPTACFVFSIL 120

Query: 226 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDH-LYDPPIDSSKLAHTDR 285
           IFLLGRRFYICKTPQGSVFAD+AKVLVATCRKRNVPKFTQIDH L+DPP+DSSKL+HTDR
Sbjct: 121 IFLLGRRFYICKTPQGSVFADIAKVLVATCRKRNVPKFTQIDHFLHDPPMDSSKLSHTDR 180

Query: 286 FLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQ 345
           FLVFDKAATV DSSTELDEQGKSKNEWKLCSVHQVEQ KCVVGIVPIWAAGIPCFISMQQ
Sbjct: 181 FLVFDKAATVVDSSTELDEQGKSKNEWKLCSVHQVEQFKCVVGIVPIWAAGIPCFISMQQ 240

Query: 346 MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFS 405
           MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFS
Sbjct: 241 MGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFS 300

Query: 406 MKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAA 465
           +KTRIEIGIVMSVLCMVVAGILEN+RRSSA ENK FVSPLHVW LIPEFALSGLTEAFAA
Sbjct: 301 LKTRIEIGIVMSVLCMVVAGILENIRRSSAEENKSFVSPLHVWMLIPEFALSGLTEAFAA 360

Query: 466 IAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDL 525
           IAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKS WLGGNDL
Sbjct: 361 IAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSGWLGGNDL 420

Query: 526 NKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           NKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV
Sbjct: 421 NKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 456

BLAST of CmaCh03G004020 vs. ExPASy TrEMBL
Match: A0A6J1F0P7 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucurbita moschata OX=3662 GN=LOC111438173 PE=3 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.5e-250
Identity = 437/534 (81.84%), Postives = 478/534 (89.51%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           V F+  NETFEKLASMSLIANL+LYLHTMYNLD+VDSAYV QIW GTTNF+ LAGAFLAD
Sbjct: 24  VRFILANETFEKLASMSLIANLILYLHTMYNLDNVDSAYVFQIWGGTTNFSPLAGAFLAD 83

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 146
           AYLGRFYTLLFGS AS LGMGVLTLGA  PEMRPPPC  G+++C Q   WQL FLYLGLG
Sbjct: 84  AYLGRFYTLLFGSIASFLGMGVLTLGAALPEMRPPPCSSGKTDCIQSKSWQLAFLYLGLG 143

Query: 147 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 206
           LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWY LFS+ALVIALTLVVY+QT+
Sbjct: 144 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSIALVIALTLVVYIQTN 203

Query: 207 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 266
           ISWTLGFAIPTA F+FSI+IFLLGR  YIC+ PQGSVFADMAKVL+AT RKR +P+    
Sbjct: 204 ISWTLGFAIPTAAFIFSIVIFLLGRHLYICRIPQGSVFADMAKVLIATYRKRQIPR-PSP 263

Query: 267 DHLYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVV 326
           +HL+DPP+DSSKL HT+RFLVFDKAA V DSS ELDEQG+SKNEWKLC+VHQVEQLKCVV
Sbjct: 264 NHLHDPPMDSSKLPHTNRFLVFDKAAMVIDSSMELDEQGRSKNEWKLCTVHQVEQLKCVV 323

Query: 327 GIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIY 386
           GIVPIWAAGI CFISMQQMGSFGILQAIQMNRF+GPHF+IPPAWMSL PMIALS+WIYIY
Sbjct: 324 GIVPIWAAGISCFISMQQMGSFGILQAIQMNRFLGPHFEIPPAWMSLIPMIALSLWIYIY 383

Query: 387 EKYVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHV 446
           EKYVEFM++KTH NKRFSMK RIEIGIVMSVLCM+VAGILE +RR SA+EN+ FVSPLHV
Sbjct: 384 EKYVEFMRRKTHHNKRFSMKMRIEIGIVMSVLCMIVAGILEKIRRDSAIENRTFVSPLHV 443

Query: 447 WTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVS 506
             LIPEFALSGLTEAFAAI+VMELLTT LPESLRTVAGAIFFLSLSL SY+SSVLM IV+
Sbjct: 444 SVLIPEFALSGLTEAFAAISVMELLTTQLPESLRTVAGAIFFLSLSLASYLSSVLMGIVN 503

Query: 507 KINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           ++++    K+ WL GNDLNKN LDYFFYVVGVIAA NFFYFRF  T FLP+ +V
Sbjct: 504 RVSKTGDSKTSWLEGNDLNKNHLDYFFYVVGVIAALNFFYFRFVGTRFLPEGDV 556

BLAST of CmaCh03G004020 vs. ExPASy TrEMBL
Match: A0A6J1DBD9 (protein NRT1/ PTR FAMILY 2.8-like OS=Momordica charantia OX=3673 GN=LOC111019435 PE=3 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 6.5e-230
Identity = 405/538 (75.28%), Postives = 457/538 (84.94%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           V F+  NETFEKLASMSL+ANL+LYLHTMYNLD+V SA V+ IW GTTNFA +AGAFLAD
Sbjct: 29  VRFILANETFEKLASMSLVANLILYLHTMYNLDNVASANVINIWGGTTNFAPIAGAFLAD 88

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 146
           AYLGRFYTLLFGS AS  GMGVLTLGAG PE+RPP C H ++ CPQP PWQL FLYLGLG
Sbjct: 89  AYLGRFYTLLFGSIASFFGMGVLTLGAGLPELRPPSCSHSQAKCPQPQPWQLAFLYLGLG 148

Query: 147 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 206
           LLVIGAGGIRPCNISFGADQFDT T KGRAKLESFLNWWY LFS+AL++ALT+VVY+QT+
Sbjct: 149 LLVIGAGGIRPCNISFGADQFDTTTYKGRAKLESFLNWWYFLFSIALIVALTVVVYIQTN 208

Query: 207 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 266
           ISW  GFAIPTACFV SILIFLLGR  YI KTPQGSVFADMAKV++ATCRK  + K    
Sbjct: 209 ISWVTGFAIPTACFVLSILIFLLGRGTYIRKTPQGSVFADMAKVIIATCRKSKLSKPNNQ 268

Query: 267 DHLYDPP---IDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLK 326
           ++L+DPP    DS+KL HT+RFLVFDKAATV D   ELDE G S + W+LCSV QVEQLK
Sbjct: 269 NNLHDPPPTIPDSTKLPHTNRFLVFDKAATVVDPVAELDEHGNSVSHWRLCSVQQVEQLK 328

Query: 327 CVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWI 386
           CV+GIVP+W AGI CFI+MQQMGSFG+LQAIQM+RF+G HF+IPPAWM+LTPMIALSIWI
Sbjct: 329 CVIGIVPVWVAGISCFIAMQQMGSFGVLQAIQMDRFLGRHFEIPPAWMNLTPMIALSIWI 388

Query: 387 YIYEKYVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSP 446
           ++YEKYV  MK+KT +NKR SM+ RIEIGIVMSV+CMVV+GI E +RR  AV N  FV+ 
Sbjct: 389 FVYEKYVAMMKRKTGQNKRISMQRRIEIGIVMSVVCMVVSGITEKLRRDLAVGNGSFVAR 448

Query: 447 LHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMA 506
           LHV  L PEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVL+ 
Sbjct: 449 LHVTALXPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLVG 508

Query: 507 IVSKIN-RVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           I+SKI+  ++G K+ W+GGNDLN NRL+YFFYVV VI   NFFYFRFFA  FLPK +V
Sbjct: 509 IISKISTTIDGKKTAWIGGNDLNHNRLEYFFYVVAVIGTLNFFYFRFFAAGFLPKSDV 566

BLAST of CmaCh03G004020 vs. ExPASy TrEMBL
Match: A0A1S4DX14 (protein NRT1/ PTR FAMILY 2.8-like OS=Cucumis melo OX=3656 GN=LOC103491761 PE=3 SV=1)

HSP 1 Score: 786.6 bits (2030), Expect = 7.0e-224
Identity = 388/455 (85.27%), Postives = 415/455 (91.21%), Query Frame = 0

Query: 106 MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 165
           MGVLTLGAG P+MRPPPC  GESNCPQP PWQL FLYLGLGL+VIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGLPQMRPPPCISGESNCPQPLPWQLSFLYLGLGLIVIGAGGIRPCNISFGAD 60

Query: 166 QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 225
           QFDTNTEKGRAKLESFLNWWY LFSVALVIALT+VVY+QT++SWTLGF IPTACFVFSI 
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYFLFSVALVIALTIVVYVQTNVSWTLGFGIPTACFVFSIS 120

Query: 226 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 285
           IFLLG R+YICK PQGSVFAD+AKV+VATCRKR +PK TQ D L++PP++SSKLAHT+RF
Sbjct: 121 IFLLGHRYYICKAPQGSVFADIAKVVVATCRKRQIPKPTQFDQLHNPPMNSSKLAHTNRF 180

Query: 286 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 345
           L+FDKAATV DSS ELDE GKSKNEW+LCSVHQVE+ KCVVGI+P+W AGIPCF+SMQQM
Sbjct: 181 LIFDKAATVVDSSVELDEDGKSKNEWRLCSVHQVEEFKCVVGIIPVWLAGIPCFMSMQQM 240

Query: 346 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 405
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMI LSIWIYIYEKYVE MKKKT  NKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMITLSIWIYIYEKYVEHMKKKTRSNKRFSM 300

Query: 406 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 465
           KTRIEIGIVMSVLCMVVAGILE +RR +AVENK F+SPLHVW LIPEFALSGLTEAFAAI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILEKLRRDAAVENKSFISPLHVWVLIPEFALSGLTEAFAAI 360

Query: 466 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 525
           AVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVL  IVSK+ R      EWLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLTGIVSKVER------EWLGGNDLN 420

Query: 526 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           KNRLDYFFYVVGVIAA NFFYFRFFA  FLPKP+V
Sbjct: 421 KNRLDYFFYVVGVIAALNFFYFRFFAGAFLPKPDV 449

BLAST of CmaCh03G004020 vs. NCBI nr
Match: XP_023544027.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1028.5 bits (2658), Expect = 2.2e-296
Identity = 515/534 (96.44%), Postives = 523/534 (97.94%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           V F+ GNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYV QIWSGTTNFAALAGAFLAD
Sbjct: 23  VRFILGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVFQIWSGTTNFAALAGAFLAD 82

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 146
           AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG
Sbjct: 83  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 142

Query: 147 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 206
           LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVY+QTS
Sbjct: 143 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYVQTS 202

Query: 207 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 266
           ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI
Sbjct: 203 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 262

Query: 267 DHLYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVV 326
           DHL+DPP+DSSKL+HTDRFLVFDKAATV DSSTELDEQGKSKNEWKLCSVHQVEQ KCVV
Sbjct: 263 DHLHDPPMDSSKLSHTDRFLVFDKAATVVDSSTELDEQGKSKNEWKLCSVHQVEQFKCVV 322

Query: 327 GIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIY 386
           GIVPIW AGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIY
Sbjct: 323 GIVPIWVAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIY 382

Query: 387 EKYVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHV 446
           EK+VEFMKKK HKNKRFSMKTRIEIGIVMSV+CMVVAGILENVRR+SAVENK FVSPLHV
Sbjct: 383 EKFVEFMKKKNHKNKRFSMKTRIEIGIVMSVICMVVAGILENVRRNSAVENKSFVSPLHV 442

Query: 447 WTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVS 506
           W LIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVS
Sbjct: 443 WMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVS 502

Query: 507 KINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           KINRVEGGKS WLGGNDLNKNRLDYFFYVVGVIAAFNF YFRFFATHFLPKPNV
Sbjct: 503 KINRVEGGKSGWLGGNDLNKNRLDYFFYVVGVIAAFNFLYFRFFATHFLPKPNV 556

BLAST of CmaCh03G004020 vs. NCBI nr
Match: KAG6603629.1 (Protein NRT1/ PTR FAMILY 2.8, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1027.3 bits (2655), Expect = 4.9e-296
Identity = 517/535 (96.64%), Postives = 524/535 (97.94%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           V F+ GNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYV QIWSGTTNFAALAGAFLAD
Sbjct: 23  VRFILGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVFQIWSGTTNFAALAGAFLAD 82

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 146
           AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG
Sbjct: 83  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 142

Query: 147 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 206
           LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVY+QTS
Sbjct: 143 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYVQTS 202

Query: 207 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 266
           ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI
Sbjct: 203 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 262

Query: 267 DH-LYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCV 326
           DH L+DPP+DSSKL+HTDRFLVFDKAATV DSSTELDEQGKSKNEWKLCSVHQVEQ KC 
Sbjct: 263 DHFLHDPPMDSSKLSHTDRFLVFDKAATVVDSSTELDEQGKSKNEWKLCSVHQVEQFKCF 322

Query: 327 VGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYI 386
           VGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYI
Sbjct: 323 VGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYI 382

Query: 387 YEKYVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLH 446
           YEKYVEFMKKKTHKNKRFS+KTRIEIGIVMSVLCMVVAGILEN+RRSSA ENK FVSPLH
Sbjct: 383 YEKYVEFMKKKTHKNKRFSLKTRIEIGIVMSVLCMVVAGILENIRRSSAEENKSFVSPLH 442

Query: 447 VWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIV 506
           VW LIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIV
Sbjct: 443 VWMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIV 502

Query: 507 SKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           SKINRVEGGKS WLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV
Sbjct: 503 SKINRVEGGKSGWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 557

BLAST of CmaCh03G004020 vs. NCBI nr
Match: XP_038881205.1 (protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida] >XP_038881206.1 protein NRT1/ PTR FAMILY 2.8-like isoform X1 [Benincasa hispida])

HSP 1 Score: 925.6 bits (2391), Expect = 2.0e-265
Identity = 459/534 (85.96%), Postives = 492/534 (92.13%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           V F+ GNETFEKLASMSLI NLVLYLHTMYNLD+V SAYV QIW GTTNFA LAGAFLAD
Sbjct: 25  VRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTTNFAPLAGAFLAD 84

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 146
           AYLGRFYTLLFGS AS LGMGVL+LGAG P+MRPPPC  GES+CPQP PWQLGFLYLGLG
Sbjct: 85  AYLGRFYTLLFGSIASFLGMGVLSLGAGLPQMRPPPCISGESSCPQPQPWQLGFLYLGLG 144

Query: 147 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 206
           L+V+GAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFS+ALVIALT+VVY+QT+
Sbjct: 145 LIVVGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSIALVIALTIVVYVQTN 204

Query: 207 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 266
           ISWTLGFAIPTACFV SILIFL GRRFY+CK PQGSVF+D+AKV+VA  RKR +PK  QI
Sbjct: 205 ISWTLGFAIPTACFVVSILIFLSGRRFYVCKAPQGSVFSDLAKVVVAMFRKRKIPKPNQI 264

Query: 267 DHLYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVV 326
           DHL++PP++S KLAHTDRFLVFDKAATV DSS ELDE+GKSK+EW+LCSVHQVEQ KCVV
Sbjct: 265 DHLHNPPMNSCKLAHTDRFLVFDKAATVVDSSIELDEEGKSKDEWRLCSVHQVEQFKCVV 324

Query: 327 GIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIY 386
           GI+P+W AGI CFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIY
Sbjct: 325 GIIPVWLAGIACFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIY 384

Query: 387 EKYVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHV 446
           EKYVEFMKKKTH NKRFSMKTRIEIGIVMSVLCMVVAG+LE +RR +AVENK FVSPLHV
Sbjct: 385 EKYVEFMKKKTHSNKRFSMKTRIEIGIVMSVLCMVVAGLLEKLRRDAAVENKSFVSPLHV 444

Query: 447 WTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVS 506
           W LIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSL SY+SSVLM IVS
Sbjct: 445 WMLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLASYLSSVLMGIVS 504

Query: 507 KINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           KI R+E G+ +WLGGNDLNKNRLDYFFYVVG IAA NFFYFRFFA  FLP P+V
Sbjct: 505 KIGRMEAGEMQWLGGNDLNKNRLDYFFYVVGGIAALNFFYFRFFAAGFLPSPDV 558

BLAST of CmaCh03G004020 vs. NCBI nr
Match: XP_022978168.1 (protein NRT1/ PTR FAMILY 2.8-like [Cucurbita maxima])

HSP 1 Score: 914.4 bits (2362), Expect = 4.6e-262
Identity = 455/455 (100.00%), Postives = 455/455 (100.00%), Query Frame = 0

Query: 106 MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 165
           MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD
Sbjct: 1   MGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLGLLVIGAGGIRPCNISFGAD 60

Query: 166 QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 225
           QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL
Sbjct: 61  QFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSISWTLGFAIPTACFVFSIL 120

Query: 226 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 285
           IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF
Sbjct: 121 IFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQIDHLYDPPIDSSKLAHTDRF 180

Query: 286 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 345
           LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM
Sbjct: 181 LVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLKCVVGIVPIWAAGIPCFISMQQM 240

Query: 346 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 405
           GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM
Sbjct: 241 GSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIALSIWIYIYEKYVEFMKKKTHKNKRFSM 300

Query: 406 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 465
           KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI
Sbjct: 301 KTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFVSPLHVWTLIPEFALSGLTEAFAAI 360

Query: 466 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 525
           AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN
Sbjct: 361 AVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVLMAIVSKINRVEGGKSEWLGGNDLN 420

Query: 526 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 561
           KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV
Sbjct: 421 KNRLDYFFYVVGVIAAFNFFYFRFFATHFLPKPNV 455

BLAST of CmaCh03G004020 vs. NCBI nr
Match: XP_004142186.1 (protein NRT1/ PTR FAMILY 2.8 [Cucumis sativus] >KAE8649550.1 hypothetical protein Csa_017951 [Cucumis sativus])

HSP 1 Score: 902.9 bits (2332), Expect = 1.4e-258
Identity = 453/546 (82.97%), Postives = 483/546 (88.46%), Query Frame = 0

Query: 15  SEDGEPSDSYLFVGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTT 74
           S + E    +  V F+ GNETFEKLASMSLI NLVLYLHTMYNLD+V SAYV QIW GTT
Sbjct: 14  SRNKETRGGWRAVRFILGNETFEKLASMSLIGNLVLYLHTMYNLDNVASAYVFQIWGGTT 73

Query: 75  NFAALAGAFLADAYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPH 134
           NFA LAGAFLADAYLGRFYTLLFGS ASLLGMGVLTLGAG P+MRPPPC  GESNCPQP 
Sbjct: 74  NFAPLAGAFLADAYLGRFYTLLFGSVASLLGMGVLTLGAGLPQMRPPPCISGESNCPQPL 133

Query: 135 PWQLGFLYLGLGLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALV 194
           PWQLGFLYLGLGL+VIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWY LFS++LV
Sbjct: 134 PWQLGFLYLGLGLIVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYFLFSISLV 193

Query: 195 IALTLVVYLQTSISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVAT 254
           IALT+VVY+QT+ISWTLGF IPTACFVFSI IFLLG R+YICK PQGSVFADMAKV+VAT
Sbjct: 194 IALTIVVYVQTNISWTLGFGIPTACFVFSIFIFLLGHRYYICKAPQGSVFADMAKVVVAT 253

Query: 255 CRKRNVPKFTQIDHLYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLC 314
           CRK  +PK TQ D L++PP++SSKLAHT+RFL+FDKAATV DSS ELDE+GKSKNEW+LC
Sbjct: 254 CRKWQIPKTTQFDQLHNPPMNSSKLAHTNRFLIFDKAATVVDSSVELDEEGKSKNEWRLC 313

Query: 315 SVHQVEQLKCVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLT 374
           SVHQVEQ KCVVGI P+W AGIPCF+SMQQM SFGILQAIQMNR IGPHFQIPPAWMSLT
Sbjct: 314 SVHQVEQFKCVVGIFPVWLAGIPCFMSMQQMSSFGILQAIQMNRSIGPHFQIPPAWMSLT 373

Query: 375 PMIALSIWIYIYEKYVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSA 434
           PMIALSIWIYIYEKYVE MKKKT  NKRFSMKTRIEIGIVMSVLCMVVAG+LE  RR +A
Sbjct: 374 PMIALSIWIYIYEKYVEHMKKKTQSNKRFSMKTRIEIGIVMSVLCMVVAGVLEKFRRDAA 433

Query: 435 VENKLFVSPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLG 494
           VENK F+SPLHVW LIPEFALSGLTEAF AIAVMELLTTHLPESLRTVAGAIFF SLSL 
Sbjct: 434 VENKSFISPLHVWVLIPEFALSGLTEAFGAIAVMELLTTHLPESLRTVAGAIFFFSLSLA 493

Query: 495 SYMSSVLMAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHF 554
           SY+SSVL  IV  + R      EWLGGNDLNKNRLDYFF VVGVIAA NFFYFRFFA  F
Sbjct: 494 SYLSSVLTGIVRGVER------EWLGGNDLNKNRLDYFFSVVGVIAALNFFYFRFFAVAF 553

Query: 555 LPKPNV 561
           LPKP+V
Sbjct: 554 LPKPDV 553

BLAST of CmaCh03G004020 vs. TAIR 10
Match: AT5G28470.1 (Major facilitator superfamily protein )

HSP 1 Score: 536.6 bits (1381), Expect = 2.4e-152
Identity = 278/533 (52.16%), Postives = 366/533 (68.67%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           + ++  NE+FEKLASMSLI NL +YL T YNL  V    V+ IW G+ N   LAGAF++D
Sbjct: 24  IKYIIANESFEKLASMSLIGNLSVYLMTKYNLGGVFLVNVINIWFGSCNILTLAGAFVSD 83

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGESNCPQPHPWQLGFLYLGLG 146
           AYLGRF+TLL GS AS +GMG+  L A  P +RP  C    +   QP  WQLG L+ GLG
Sbjct: 84  AYLGRFWTLLLGSIASFIGMGIFALTAALPSLRPDACIDPSNCSNQPAKWQLGVLFSGLG 143

Query: 147 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 206
           LL IGAGG+RPCNI+FGADQFDT+T+KG+A LE+F NWWY  F+VALVIALT VVY+QT+
Sbjct: 144 LLAIGAGGVRPCNIAFGADQFDTSTKKGKAHLETFFNWWYFSFTVALVIALTGVVYIQTN 203

Query: 207 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 266
           ISW +GF IPTAC   SI  F++G+  YIC   +GSVFAD+ KV+ A C+KR V   + I
Sbjct: 204 ISWVIGFVIPTACLALSITTFVIGQHTYICAKAEGSVFADIVKVVTAACKKRKVKPGSDI 263

Query: 267 DHLYDPPIDSSK---LAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLK 326
                P  D S    +    R   FDKA+ V  +  EL+E G +K +W+LCSV QV+ LK
Sbjct: 264 TFYIGPSNDGSPTTLVRDKHRLRFFDKASIV-TNPNELNEDGNAKYKWRLCSVQQVKNLK 323

Query: 327 CVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPH-FQIPPAWMSLTPMIALSIW 386
           CV  I+P+W  GI CFI   Q   +GILQA+QM++  GPH FQ+P  WM+L  MI L+IW
Sbjct: 324 CVTAILPVWVTGIACFILTDQQNIYGILQAMQMDKTFGPHNFQVPAGWMNLVSMITLAIW 383

Query: 387 IYIYE-KYVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFV 446
           I +YE   +  +K+ T + KR ++K RIE  IVM ++CM+VAG  E  RR+SA++N  FV
Sbjct: 384 ISLYECVIIPIVKQITGRKKRLTLKHRIE--IVMGIICMIVAGFQEKKRRASALKNGSFV 443

Query: 447 SPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVL 506
           SP+ +  L+P+FAL+GLTEAF+A+A+ME LT  +PE +R VAGAIFFLS S+ SY+ ++L
Sbjct: 444 SPVSIVMLLPQFALAGLTEAFSAVALMEFLTVRMPEHMRAVAGAIFFLSSSIASYICTLL 503

Query: 507 MAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHF 555
           + ++  + R EG    WLG  DLNKNRL+ +F+++  I   N  YFR FA+ +
Sbjct: 504 INVIDAVTRKEG--KSWLGDKDLNKNRLENYFFIIAGIQVANLLYFRLFASRY 551

BLAST of CmaCh03G004020 vs. TAIR 10
Match: AT1G27080.1 (nitrate transporter 1.6 )

HSP 1 Score: 405.2 bits (1040), Expect = 8.5e-113
Identity = 216/537 (40.22%), Postives = 343/537 (63.87%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           + F+ GNET EKL S+ + AN +LYL  +++++ V++  V  +W G TNFA L GA ++D
Sbjct: 21  ITFILGNETLEKLGSIGVSANFMLYLRNVFHMEPVEAFNVYYLWMGLTNFAPLLGALISD 80

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGE-SNCPQPHPWQLGFLYLGL 146
           AY+GRF T+ + S  S+LG+  +TL A  P++ PPPC +     C  P+  QLG L+LGL
Sbjct: 81  AYIGRFKTIAYASLFSILGLMTVTLTACLPQLHPPPCNNPHPDECDDPNKLQLGILFLGL 140

Query: 147 GLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQT 206
           G L IG+GGIRPC+I FG DQFD  TE+G   + SF NW+YL  ++ L+ + T+VVYLQT
Sbjct: 141 GFLSIGSGGIRPCSIPFGVDQFDQRTEQGLKGVASFFNWYYLTLTMVLIFSHTVVVYLQT 200

Query: 207 SISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQ 266
            +SW +GF+IPT+    ++++F +G RFY+   P+GSVF+ +A+V+VA  +KR++ K + 
Sbjct: 201 -VSWVIGFSIPTSLMACAVVLFFVGMRFYVYVKPEGSVFSGIARVIVAARKKRDL-KISL 260

Query: 267 ID----HLYDPPIDS---SKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQ 326
           +D      Y+PP+     SKL  TD+F   DKAA + D   +L  +G   N+W+LCS+ +
Sbjct: 261 VDDGTEEYYEPPVKPGVLSKLPLTDQFKFLDKAAVILDG--DLTSEGVPANKWRLCSIQE 320

Query: 327 VEQLKCVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIA 386
           VE++KC++ +VP+W+AGI   ++M    +F + QA +M+R +GPHF+IP A +++   I 
Sbjct: 321 VEEVKCLIRVVPVWSAGIISIVAMTTQATFMVFQATKMDRHMGPHFEIPAASITVISYIT 380

Query: 387 LSIWIYIYEK-YVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVEN 446
           + IW+ IYE   V F+ +   +  R ++  R+ IGIV ++L M  AG +E VRR+ A E 
Sbjct: 381 IGIWVPIYEHLLVPFLWRM--RKFRVTLLQRMGIGIVFAILSMFTAGFVEGVRRTRATE- 440

Query: 447 KLFVSPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYM 506
              ++ + V+ L     L GL E+F  I ++E   +  PE +R++A ++F LS +  +Y+
Sbjct: 441 ---MTQMSVFWLALPLILMGLCESFNFIGLIEFFNSQFPEHMRSIANSLFPLSFAAANYL 500

Query: 507 SSVLMAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHF 555
           SS+L+  V K++  +    +WL   DL++ +LDYF+Y++ V+   N  YF + A  +
Sbjct: 501 SSLLVTTVHKVSGTK-DHPDWL-NKDLDRGKLDYFYYLIAVLGVVNLVYFWYCAHRY 545

BLAST of CmaCh03G004020 vs. TAIR 10
Match: AT1G69870.1 (nitrate transporter 1.7 )

HSP 1 Score: 404.8 bits (1039), Expect = 1.1e-112
Identity = 217/537 (40.41%), Postives = 342/537 (63.69%), Query Frame = 0

Query: 27  VGFVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLAD 86
           V F+ GNET E+L S+ L+AN ++YL  +++L+ VD+A V+ IWSG TN   L GA+++D
Sbjct: 58  VSFILGNETLERLGSIGLLANFMVYLTKVFHLEQVDAANVINIWSGFTNLTPLVGAYISD 117

Query: 87  AYLGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGES-NCPQPHPWQLGFLYLGL 146
            Y+GRF T+ F SFA+LLG+  +TL A FP++ P  C   +  +C  P+  Q+G L LGL
Sbjct: 118 TYVGRFKTIAFASFATLLGLITITLTASFPQLHPASCNSQDPLSCGGPNKLQIGVLLLGL 177

Query: 147 GLLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQT 206
             L +G+GGIRPC+I FG DQFD  TE+G   + SF NW+Y+ F+V L+I  T+VVY+Q 
Sbjct: 178 CFLSVGSGGIRPCSIPFGVDQFDQRTEEGVKGVASFFNWYYMTFTVVLIITQTVVVYIQD 237

Query: 207 SISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQ 266
            +SW +GF+IPT     ++++F  G + Y+   P+GS+F+ +A+V+VA  +KR +    +
Sbjct: 238 QVSWIIGFSIPTGLMALAVVMFFAGMKRYVYVKPEGSIFSGIAQVIVAARKKRKLKLPAE 297

Query: 267 ID---HLYDPPIDS---SKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQV 326
            D     YDP I S   SKL  +++F   DKAA V +   +L  +G   ++W+LCSV +V
Sbjct: 298 DDGTVTYYDPAIKSSVLSKLHRSNQFRCLDKAAVVIEG--DLTPEGPPADKWRLCSVQEV 357

Query: 327 EQLKCVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGPHFQIPPAWMSLTPMIAL 386
           E++KC++ IVPIW+AGI    +M   G+F + QA++M+R +GP F+IP   +S+  ++ +
Sbjct: 358 EEVKCLIRIVPIWSAGIISLAAMTTQGTFTVSQALKMDRNLGPKFEIPAGSLSVISLLTI 417

Query: 387 SIWIYIYEK-YVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVE-- 446
            I++  Y++ +V FM++ T      ++  RI  GIV ++  M+VAGI+E +RR  ++   
Sbjct: 418 GIFLPFYDRVFVPFMRRITGHKSGITLLQRIGTGIVFAIFSMIVAGIVERMRRIRSINAG 477

Query: 447 NKLFVSPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSY 506
           +   ++P+ V+ L P+  L GL EAF  I  +E   +  PE +R++A ++F LS +  SY
Sbjct: 478 DPTGMTPMSVFWLSPQLILMGLCEAFNIIGQIEFFNSQFPEHMRSIANSLFSLSFAGSSY 537

Query: 507 MSSVLMAIVSKINRVEGG--KSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFA 552
           +SS L+ +V K +   GG  + +WL  N LN  +LDYF+Y++ V+   N  YF + A
Sbjct: 538 LSSFLVTVVHKFS---GGHDRPDWLNKN-LNAGKLDYFYYLIAVLGVVNLVYFWYCA 588

BLAST of CmaCh03G004020 vs. TAIR 10
Match: AT5G62680.1 (Major facilitator superfamily protein )

HSP 1 Score: 356.3 bits (913), Expect = 4.5e-98
Identity = 202/534 (37.83%), Postives = 313/534 (58.61%), Query Frame = 0

Query: 29  FVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLADAY 88
           F+ GNETFEKL  +  ++NL++YL  ++NL S+ +A ++  +SGT NF     AFL D Y
Sbjct: 52  FIIGNETFEKLGIIGTLSNLLVYLTAVFNLKSITAATIINAFSGTINFGTFVAAFLCDTY 111

Query: 89  LGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPC-RHGESNCPQPHPWQLGFLYLGLGL 148
            GR+ TL     A  LG  V+ L A  P++ P  C    +S C  P   Q+ FL +GLG 
Sbjct: 112 FGRYKTLSVAVIACFLGSFVILLTAAVPQLHPAACGTAADSICNGPSGGQIAFLLMGLGF 171

Query: 149 LVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTSI 208
           LV+GAGGIRPCN++FGADQF+  +E G+  ++SF NW++  F+ A +++LTLVVY+Q+++
Sbjct: 172 LVVGAGGIRPCNLAFGADQFNPKSESGKRGIDSFFNWYFFTFTFAQILSLTLVVYVQSNV 231

Query: 209 SWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQ-- 268
           SWT+G  IP      + LIF  G + Y+     GS  A +A+V+    +KR +    Q  
Sbjct: 232 SWTIGLTIPAVLMFLACLIFFAGDKLYVKIKASGSPLAGIAQVIAVAIKKRGLKPAKQPW 291

Query: 269 --IDHLYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLK 328
             + + Y P   +SKL +TD+F   DKAA +     +L   GK  + WKLC++ QVE++K
Sbjct: 292 LNLYNYYPPKYANSKLKYTDQFRFLDKAA-ILTPEDKLQPDGKPADPWKLCTMQQVEEVK 351

Query: 329 CVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMNRFIGP-HFQIPPAWMSLTPMIALSIW 388
           C+V ++PIW A    ++++ Q  ++ + QA+Q +R +G   F IP A   +  M  ++++
Sbjct: 352 CIVRVLPIWFASSIYYLTITQQMTYPVFQALQSDRRLGSGGFVIPAATYVVFLMTGMTVF 411

Query: 389 IYIYEK-YVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAV------ 448
           I +Y++  V  M++ T  +   ++  RI  GI  +   +VVAG +E  RR+ A+      
Sbjct: 412 IVVYDRVLVPTMRRITGLDTGITLLQRIGTGIFFATASLVVAGFVEERRRTFALTKPTLG 471

Query: 449 --ENKLFVSPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSL 508
               K  +S +    LIP+ +L+G+ EAFAAI  ME      PE++R+ AG+IF++   +
Sbjct: 472 MAPRKGEISSMSAMWLIPQLSLAGVAEAFAAIGQMEFYYKQFPENMRSFAGSIFYVGGGV 531

Query: 509 GSYMSSVLMAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYF 548
            SY+ S L+A V +  +   G   WL   DLNK RLD F++++  I A NF YF
Sbjct: 532 SSYLGSFLIATVHRTTQNSSG-GNWL-AEDLNKGRLDLFYFMIAGILAVNFAYF 582

BLAST of CmaCh03G004020 vs. TAIR 10
Match: AT1G69860.1 (Major facilitator superfamily protein )

HSP 1 Score: 349.0 bits (894), Expect = 7.2e-96
Identity = 194/533 (36.40%), Postives = 324/533 (60.79%), Query Frame = 0

Query: 29  FVEGNETFEKLASMSLIANLVLYLHTMYNLDSVDSAYVLQIWSGTTNFAALAGAFLADAY 88
           ++ GNET E+LA+  L+AN ++Y+   Y++D V +  ++  WS  TNFA + GAF++D+Y
Sbjct: 27  YIIGNETLERLATFGLMANFMVYMVREYHMDQVQAVTLINTWSALTNFAPIIGAFISDSY 86

Query: 89  LGRFYTLLFGSFASLLGMGVLTLGAGFPEMRPPPCRHGE--SNCPQPHPWQLGFLYLGLG 148
            G+F T++FGS A LLGM VLT  +  P +RPPPC   +    C      QL  L  GL 
Sbjct: 87  TGKFNTIVFGSIAELLGMLVLTFTSLVPNLRPPPCTADQITGQCIPYSYSQLYVLLSGLF 146

Query: 149 LLVIGAGGIRPCNISFGADQFDTNTEKGRAKLESFLNWWYLLFSVALVIALTLVVYLQTS 208
           LL +G GGIR C++ F  DQFD +TE+GR    SF +W+Y   ++  ++++TLV+Y+Q +
Sbjct: 147 LLSVGTGGIRSCSVPFSLDQFDDSTEEGREGSRSFFSWYYTTHTIVQLVSMTLVLYVQNN 206

Query: 209 ISWTLGFAIPTACFVFSILIFLLGRRFYICKTPQGSVFADMAKVLVATCRKRNVPKFTQI 268
           ISW +GFAIPT    F++L+  +G R+Y+   P+GSVF+ + KVLVA  +KR     + I
Sbjct: 207 ISWGIGFAIPTVLNFFALLLLFVGTRYYVFVKPEGSVFSGVFKVLVAAYKKRKARFTSGI 266

Query: 269 DH---LYDPPIDSSKLAHTDRFLVFDKAATVFDSSTELDEQGKSKNEWKLCSVHQVEQLK 328
           D+   L +  + S+KL  TD+F   +KA  V ++    DE G    EW+ C+V Q+E +K
Sbjct: 267 DYHQPLLETDLQSNKLVLTDQFRFLNKAVIVMNN----DEAG--NEEWRTCTVRQIEDIK 326

Query: 329 CVVGIVPIWAAGIPCFISMQQMGSFGILQAIQMN-RFIGPHFQIPPAWMSLTPMIALSIW 388
            ++ I+PI+A+ I  F++M Q  +F + QA++M+ +F G  + IPPA +++  ++ + IW
Sbjct: 327 SIISIIPIFASSIIGFLAMNQQQTFTVSQALKMDLQFPGTSYLIPPASITVISLLNIGIW 386

Query: 389 IYIYEK-YVEFMKKKTHKNKRFSMKTRIEIGIVMSVLCMVVAGILENVRRSSAVENKLFV 448
           +  YE   V  ++  T +N   S+  ++ IG + S+  M+++GI+E  RR  ++      
Sbjct: 387 LPFYETVLVRHIENITKQNGGISLLQKVGIGNIFSISTMLISGIVERKRRDLSLNG---- 446

Query: 449 SPLHVWTLIPEFALSGLTEAFAAIAVMELLTTHLPESLRTVAGAIFFLSLSLGSYMSSVL 508
             + V+ L P+  L G  + F  + + E     +P ++R++  ++ +L LSL SY+SS +
Sbjct: 447 VKMSVFWLTPQQVLMGFYQVFTIVGLTEFFNKQVPINMRSIGNSLLYLGLSLASYLSSAM 506

Query: 509 MAIVSKINRVEGGKSEWLGGNDLNKNRLDYFFYVVGVIAAFNFFYFRFFATHF 555
           ++IV  +    GG+S WL  +D++K++LD F+Y +  ++  NF +F + A  +
Sbjct: 507 VSIVHSVT-ARGGQS-WL-TDDIDKSKLDCFYYFIAALSTLNFIFFFWCARRY 546