CmaCh03G003240 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh03G003240 vs. ExPASy Swiss-Prot
Match: Q84K71 (Protein DETOXIFICATION 44, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX44 PE=2 SV=1)

HSP 1 Score: 569.7 bits (1467), Expect = 3.6e-161
Identity = 302/450 (67.11%), Postives = 371/450 (82.44%), Query Frame = 0

Query: 103 KLALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPL 162
           K+ ++I++IALPAALALAADPI SL+DTAFVGHIGS ELAAVGVS SVFNLVSKLFNVPL
Sbjct: 75  KIGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVPL 134

Query: 163 LNITTSFVAEEQALIHKNEKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVML 222
           LN+TTSFVAEEQA+  K++ ++++ +         KK+L SVSTSL LAAG+GIAEA+ L
Sbjct: 135 LNVTTSFVAEEQAIAAKDDNDSIETS---------KKVLPSVSTSLVLAAGVGIAEAIAL 194

Query: 223 SLGSGSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYAT 282
           SLGS  LMD+M IP  S MR PAEQFL LRA+GAPPIV+ALAAQG FRGFKDT TPLYA 
Sbjct: 195 SLGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYGAPPIVVALAAQGAFRGFKDTTTPLYAV 254

Query: 283 AAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRI 342
            AGN+LNAVLDP+LIF  GFGI GAA ATVISEYLIAF+LLW+LN  + L    I  GR 
Sbjct: 255 VAGNVLNAVLDPILIFVLGFGISGAAAATVISEYLIAFILLWKLNENVVLLSPQIKVGRA 314

Query: 343 ARYLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAG 402
            +YLKSGGLL+GRT+A+LV  TLATS+AA+ G   MAG+QI ++IWLA+SLLTDALA+A 
Sbjct: 315 NQYLKSGGLLIGRTVALLVPFTLATSLAAQNGPTQMAGHQIVLEIWLAVSLLTDALAIAA 374

Query: 403 QALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIAR 462
           Q+LLA++Y+  +Y+ +R+V++  LQ+GL +G  LA +LF+ F  FS LF+ D+EVL+IA 
Sbjct: 375 QSLLATTYSQGEYKQAREVLFGVLQVGLATGTGLAAVLFITFEPFSSLFTTDSEVLKIAL 434

Query: 463 SGLWFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVW 522
           SG  FVAGSQPV+ALAFV+DGLYYGVSDFG+AAYSMV+VG ISSL++LV  P FGL G+W
Sbjct: 435 SGTLFVAGSQPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFISSLFMLVAAPTFGLAGIW 494

Query: 523 SGLFLFMMLRLIAGIWRLGTKSGPWEMVFN 553
           +GLFLFM LRL+AG WRLGT++GPW+M+++
Sbjct: 495 TGLFLFMALRLVAGAWRLGTRTGPWKMLWS 515

BLAST of CmaCh03G003240 vs. ExPASy Swiss-Prot
Match: Q9SVE7 (Protein DETOXIFICATION 45, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX45 PE=2 SV=2)

HSP 1 Score: 409.8 bits (1052), Expect = 4.8e-113
Identity = 235/469 (50.11%), Postives = 321/469 (68.44%), Query Frame = 0

Query: 83  STFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALAADPIASLIDTAFVGHIGSTELA 142
           S+   +P +N     V +   +  +++ ++LPA    A DP+  L++TA++G +GS EL 
Sbjct: 84  SSLDKLPEVNGVHTGVARPVDIKRELVMLSLPAIAGQAIDPLTLLMETAYIGRLGSVELG 143

Query: 143 AVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH----KNEKNTVQINIDGIEENQGK 202
           + GVS ++FN +SKLFN+PLL++ TSFVAE+ A I      +E +   I   G+ E   +
Sbjct: 144 SAGVSMAIFNTISKLFNIPLLSVATSFVAEDIAKIAAQDLASEDSQSDIPSQGLPE---R 203

Query: 203 KLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPP 262
           K LSSVST+L LA GIGI EA+ LSL SG  + +MGI   S M  PA QFL LRA GAP 
Sbjct: 204 KQLSSVSTALVLAIGIGIFEALALSLASGPFLRLMGIQSMSEMFIPARQFLVLRALGAPA 263

Query: 263 IVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLI 322
            V++LA QG FRGFKDTKTP+Y    GN L   L PL I+    G+ GAAI++VIS+Y +
Sbjct: 264 YVVSLALQGIFRGFKDTKTPVYCLGIGNFLAVFLFPLFIYKFRMGVAGAAISSVISQYTV 323

Query: 323 AFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPM 382
           A ++L  LN  + L    I   +   YLKSGG ++GRTL+VLVT+T+ATSMAAR+G   M
Sbjct: 324 AILMLILLNKRVILLPPKIGSLKFGDYLKSGGFVLGRTLSVLVTMTVATSMAARQGVFAM 383

Query: 383 AGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAI 442
           A +QIC+Q+WLA+SLLTDALA +GQAL+ASS + +D+E  ++V    L+IG+++GI+LAI
Sbjct: 384 AAHQICMQVWLAVSLLTDALASSGQALIASSASKRDFEGVKEVTTFVLKIGVVTGIALAI 443

Query: 443 ILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSM 502
           +L + F + +GLFS D EVL I R G+ FVA +QP++ALAF+ DGL+YG+SDF YAA SM
Sbjct: 444 VLGMSFSSIAGLFSKDPEVLRIVRKGVLFVAATQPITALAFIFDGLHYGMSDFPYAACSM 503

Query: 503 VLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRLGTKSGPW 548
           ++VG ISS ++L      GL GVW GL +FM LR++AG  RL  + GPW
Sbjct: 504 MVVGGISSAFMLYAPAGLGLSGVWVGLSMFMGLRMVAGFSRLMWRKGPW 549

BLAST of CmaCh03G003240 vs. ExPASy Swiss-Prot
Match: Q9SYD6 (Protein DETOXIFICATION 42 OS=Arabidopsis thaliana OX=3702 GN=DTX42 PE=2 SV=2)

HSP 1 Score: 381.7 bits (979), Expect = 1.4e-104
Identity = 224/511 (43.84%), Postives = 321/511 (62.82%), Query Frame = 0

Query: 77  SSNSLLSTFVSVPLLNRFRD--AVFKFDKLALDILAIALPAALALAADPIASLIDTAFVG 136
           S +   + F   PL   F D  +V KFD+L L+I  IALPAALAL ADPIASL+DTAF+G
Sbjct: 3   SEDGYNTDFPRNPLYIFFSDFRSVLKFDELGLEIARIALPAALALTADPIASLVDTAFIG 62

Query: 137 HIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH-----KNEKNTVQINI 196
            IG  ELAAVGVS ++FN VS++   PL++ITTSFVAEE A        ++ K  ++I I
Sbjct: 63  QIGPVELAAVGVSIALFNQVSRIAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGI 122

Query: 197 DGIEEN------------------------------QGKKLLSSVSTSLALAAGIGIAEA 256
           +   E                                 K+ + S S++L +   +G+ +A
Sbjct: 123 NNPTEETIELIPEKHKDSLSDEFKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQA 182

Query: 257 VMLSLGSGSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPL 316
           V L   +  L+  MG+   S M  P++++LSLR+ GAP ++++LAAQG FRGFKDT TPL
Sbjct: 183 VFLISAAKPLLSFMGVKHDSPMMRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPL 242

Query: 317 YATAAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDG 376
           +AT  G++ N +LDP+ IF    G+ GAA A VIS+YL+  +LLW+L G++ +   S   
Sbjct: 243 FATVIGDVTNIILDPIFIFVFRLGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKH 302

Query: 377 GRIARYLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALA 436
            +  R++K+G LL+ R +AV   +TL+ S+AAREG+  MA +Q+C+Q+WLA SLL D  A
Sbjct: 303 LQFCRFMKNGFLLLMRVIAVTFCVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYA 362

Query: 437 LAGQALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLE 496
           +AGQA+LAS++  +DY+ +     R LQ+GL+ G  LA+IL  G    + +F+ D +VL 
Sbjct: 363 VAGQAILASAFAKKDYKRAAATASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLH 422

Query: 497 IARSGLWFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLP 551
           +   GL FVAG+QP++ALAFV DG+ +G SDFGYAA S+V+V ++S L LL ++   G  
Sbjct: 423 LISIGLPFVAGTQPINALAFVFDGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFI 482

BLAST of CmaCh03G003240 vs. ExPASy Swiss-Prot
Match: Q9SFB0 (Protein DETOXIFICATION 43 OS=Arabidopsis thaliana OX=3702 GN=DTX43 PE=1 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 1.4e-96
Identity = 216/515 (41.94%), Postives = 309/515 (60.00%), Query Frame = 0

Query: 76  KSSNSLLSTFVSVPLLNRFRDA--VFKFDKLALDILAIALPAALALAADPIASLIDTAFV 135
           ++ + L +    +P L  F+D   VF  D    +IL IA PAALALAADPIASLIDTAFV
Sbjct: 3   ETGDDLATVKKPIPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFV 62

Query: 136 GHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQA------------LIH--- 195
           G +G+ +LAAVGVS ++FN  S++   PL+++TTSFVAEE              L+H   
Sbjct: 63  GRLGAVQLAAVGVSIAIFNQASRITIFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAET 122

Query: 196 ---------------KNEKNTVQ--------INIDGIEENQGKKLLSSVSTSLALAAGIG 255
                           N+ N  Q         N       + K+ + + ST++ L   +G
Sbjct: 123 ILVQDSLEKGISSPTSNDTNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILG 182

Query: 256 IAEAVMLSLGSGSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDT 315
           + +A+ L   S  L+ +MG+   S M +PA ++LS+RA GAP ++++LA QG FRGFKDT
Sbjct: 183 LVQAIFLIFSSKLLLGVMGVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDT 242

Query: 316 KTPLYATAAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLS 375
           KTPL+AT   +++N VLDP+ IF    GI GAAIA VIS+Y +  +L   L  +++L   
Sbjct: 243 KTPLFATVVADVINIVLDPIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPP 302

Query: 376 SIDGGRIARYLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPMAGYQICVQIWLAISLLT 435
           +    +  R+LK+G LL+ RT+AV    TLA +MAAR GT PMA +QIC+Q+WL  SLL 
Sbjct: 303 NFGDLQFGRFLKNGLLLLARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLN 362

Query: 436 DALALAGQALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADA 495
           D LA+AGQA+LA S+  +DY     V  R LQ+G + G+ L++ + LG    +G+FS D 
Sbjct: 363 DGLAVAGQAILACSFAEKDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDP 422

Query: 496 EVLEIARSGLWFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPA 551
            V+ +   G+ F+A +QP+++LAFV+DG+ +G SDF Y AYSMV V  IS   ++ +   
Sbjct: 423 AVIHLMAIGIPFIAATQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKT 482

BLAST of CmaCh03G003240 vs. ExPASy Swiss-Prot
Match: Q8W4G3 (Protein DETOXIFICATION 46, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=DTX46 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 3.0e-14
Identity = 110/446 (24.66%), Postives = 196/446 (43.95%), Query Frame = 0

Query: 107 DILAIALPAALALAADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNIT 166
           +I+    PAA      P+ SLIDTA +G   S ELAA+G +  + + +   F    L++ 
Sbjct: 118 EIVMFTGPAAGLWLCGPLMSLIDTAVIGQGSSLELAALGPATVICDYLCYTF--MFLSVA 177

Query: 167 TSFVAEEQALIHKNEKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGS 226
           TS +      + + +K+ VQ  I             S+   + LA G+ +     L  GS
Sbjct: 178 TSNLVATS--LARQDKDEVQHQI-------------SILLFIGLACGVTMMVLTRL-FGS 237

Query: 227 GSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGN 286
            +L    G+     + A A +++ +R    P ++I   AQ    G KD+  PL A A  +
Sbjct: 238 WALTAFTGVKNADIVPA-ANKYVQIRGLAWPAVLIGWVAQSASLGMKDSWGPLKALAVAS 297

Query: 287 LLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLN--GEISLTLSSIDGGRIAR 346
            +N V D +L  F G+GI GAA AT++S+ + A++++  LN  G  + +        +  
Sbjct: 298 AINGVGDVVLCTFLGYGIAGAAWATMVSQVVAAYMMMDALNKKGYSAFSFCVPSPSELLT 357

Query: 347 YLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQA 406
                  +    ++ ++  TL    A   GT  +A +Q+ +QI+   ++  + L+   Q+
Sbjct: 358 IFGLAAPVFITMMSKVLFYTLLVYFATSMGTNIIAAHQVMLQIYTMSTVWGEPLSQTAQS 417

Query: 407 LLASSY--TLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIAR 466
            +        ++   +R ++   + IG   GI +  I       F G+F+ D  V     
Sbjct: 418 FMPELLFGINRNLPKARVLLKSLVIIGATLGIVVGTIGTAVPWLFPGIFTRDKVVTSEMH 477

Query: 467 SGL--WFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSMV-LVGLISSLYLLVVTPAFGLP 526
             +  +F+A S  ++     ++G      D  Y + SM   + +   L +L+    FGL 
Sbjct: 478 KVIIPYFLALS--ITPSTHSLEGTLLAGRDLRYISLSMTGCLAVAGLLLMLLSNGGFGLR 537

Query: 527 GVWSGLFLFMMLRLIAGIWRLGTKSG 546
           G W  L  F   R    ++RL ++ G
Sbjct: 538 GCWYALVGFQWARFSLSLFRLLSRDG 542

BLAST of CmaCh03G003240 vs. ExPASy TrEMBL
Match: A0A6J1IP82 (Protein DETOXIFICATION OS=Cucurbita maxima OX=3661 GN=LOC111478206 PE=3 SV=1)

HSP 1 Score: 1038.1 bits (2683), Expect = 1.3e-299
Identity = 559/559 (100.00%), Postives = 559/559 (100.00%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS
Sbjct: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480
           VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV
Sbjct: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540
           VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

Query: 541 GTKSGPWEMVFNELDEKSD 560
           GTKSGPWEMVFNELDEKSD
Sbjct: 541 GTKSGPWEMVFNELDEKSD 559

BLAST of CmaCh03G003240 vs. ExPASy TrEMBL
Match: A0A6J1GD47 (Protein DETOXIFICATION OS=Cucurbita moschata OX=3662 GN=LOC111453089 PE=3 SV=1)

HSP 1 Score: 1020.0 bits (2636), Expect = 3.8e-294
Identity = 548/559 (98.03%), Postives = 553/559 (98.93%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASSEQLRRNGPGKKSSNSLLS  VSVPLLNRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH N
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKNT QINIDGIEENQGKKLLSSVSTSLALAAG+GIAEAVMLSLGSGSLMDIMGIPVGSS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEIS TLSSIDGGR+ARYLKSGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540
           VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

Query: 541 GTKSGPWEMVFNELDEKSD 560
           GTKSGPWEMVFNELDEKSD
Sbjct: 541 GTKSGPWEMVFNELDEKSD 559

BLAST of CmaCh03G003240 vs. ExPASy TrEMBL
Match: A0A6J1CS71 (Protein DETOXIFICATION OS=Momordica charantia OX=3673 GN=LOC111013829 PE=3 SV=1)

HSP 1 Score: 904.0 bits (2335), Expect = 3.0e-259
Identity = 493/560 (88.04%), Postives = 523/560 (93.39%), Query Frame = 0

Query: 1   MATGLS-IYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEP 60
           MA  LS IYVPCF+T TNLSSK Q+LRRNANC+VRFR   KA FQKN TT SLKSPPEEP
Sbjct: 1   MAAALSAIYVPCFSTDTNLSSKIQMLRRNANCKVRFRSLPKALFQKNFTT-SLKSPPEEP 60

Query: 61  KSTASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALAL 120
           KS+ASSEQ+RR+ P  KSSNSLLS  VSVPLLNR RDAVFKFD+LALD+LAIALPAALAL
Sbjct: 61  KSSASSEQVRRDKPA-KSSNSLLSAPVSVPLLNRVRDAVFKFDQLALDVLAIALPAALAL 120

Query: 121 AADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHK 180
           AADPIASL+DTAFVGHIGSTELAAVGVSAS+FNLVSKLFNVPLLNITTSFVAEEQAL+  
Sbjct: 121 AADPIASLVDTAFVGHIGSTELAAVGVSASIFNLVSKLFNVPLLNITTSFVAEEQALVST 180

Query: 181 NEKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGS 240
            EK+ VQ NIDGI+ENQ KKLLSSVSTSLALAAG+GIAEAVML LGSG+LMDIMGIP  S
Sbjct: 181 KEKSIVQTNIDGIDENQEKKLLSSVSTSLALAAGLGIAEAVMLCLGSGALMDIMGIPAES 240

Query: 241 SMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFF 300
           SMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNA LDPLLIF 
Sbjct: 241 SMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAALDPLLIFL 300

Query: 301 CGFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAV 360
           CGFGIGGAAIATVISEYLIAFVLLW+LNGEIS TLSS+DG RIARYL+SGGLLMGRTLAV
Sbjct: 301 CGFGIGGAAIATVISEYLIAFVLLWKLNGEISFTLSSMDGERIARYLQSGGLLMGRTLAV 360

Query: 361 LVTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSR 420
           LVTLTLATSMAAREG VPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSR
Sbjct: 361 LVTLTLATSMAAREGPVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSR 420

Query: 421 QVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAF 480
           +VIYR LQIGLI+GISLAIILFLGFGAF+GLFSADAEVLEIARSGL+FVAGSQPV+A+AF
Sbjct: 421 EVIYRVLQIGLITGISLAIILFLGFGAFAGLFSADAEVLEIARSGLFFVAGSQPVNAMAF 480

Query: 481 VVDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWR 540
           VVDGLYYGVSDFGYAAYSMV VGL+SSL+L+V  PAFGLPGVWSGLFLFMMLRL+AGIWR
Sbjct: 481 VVDGLYYGVSDFGYAAYSMVAVGLVSSLFLIVAAPAFGLPGVWSGLFLFMMLRLVAGIWR 540

Query: 541 LGTKSGPWEMVFNELDEKSD 560
           LGTK GPWEMVFNE++ KSD
Sbjct: 541 LGTKGGPWEMVFNEVEGKSD 558

BLAST of CmaCh03G003240 vs. ExPASy TrEMBL
Match: A0A1S3CL06 (Protein DETOXIFICATION OS=Cucumis melo OX=3656 GN=LOC103502163 PE=3 SV=1)

HSP 1 Score: 891.7 bits (2303), Expect = 1.5e-255
Identity = 489/559 (87.48%), Postives = 513/559 (91.77%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIY P FNT TNLS KCQ+LRRNANC + F Y  K SF KNLTTSSLKSP EE K
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASS+Q+RRN   K SSNS LS  V     NRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSASV-----NRFRDAAFKFDELALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALI+ +
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTD 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKN VQ +ID IE+ Q KKLLSSVSTSLALA  +GIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNIVQTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGN LNA+LDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNFLNAILDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAF+LLWRLNGEIS TLSSIDGGRIARYL+SGGLLM RTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG VPMAGYQI VQIW+AISLLTDALALAGQALLA S+TLQDY+HSRQ
Sbjct: 361 VTLTLATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQALLAGSFTLQDYKHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480
           VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLE ARSG  FVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540
           VDGLYYGVSDFGYAAYSMVLVGL+SS++LLVVTP FGLPGVWSGLFLFMMLRL+AGIWRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWRL 540

Query: 541 GTKSGPWEMVFNELDEKSD 560
           GTKSGPWE+VFNE+D KSD
Sbjct: 541 GTKSGPWELVFNEVDGKSD 554

BLAST of CmaCh03G003240 vs. ExPASy TrEMBL
Match: A0A5D3CIE7 (Protein DETOXIFICATION OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold107G00260 PE=3 SV=1)

HSP 1 Score: 853.6 bits (2204), Expect = 4.7e-244
Identity = 473/542 (87.27%), Postives = 495/542 (91.33%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIY P FNT TNLS KCQ+LRRNANC + F Y  K SF KNLTTSSLKSP EE K
Sbjct: 1   MATGLSIYAPSFNTHTNLSFKCQMLRRNANCGLLFPYLPKPSFHKNLTTSSLKSPLEESK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASS+Q+RRN   K SSNS LS  V     NRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSDQVRRNRLDKNSSNSFLSASV-----NRFRDAAFKFDELALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALI+ +
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALINTD 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKN VQ +ID IE+ Q KKLLSSVSTSLALA  +GIAEAVMLSLGSG+LMDIMGIPV SS
Sbjct: 181 EKNIVQTDIDDIEDTQEKKLLSSVSTSLALATCLGIAEAVMLSLGSGTLMDIMGIPVDSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNA+LDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAILDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAF+LLWRLNGEIS TLSSIDGGRIARYL+SGGLLM RTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFILLWRLNGEISFTLSSIDGGRIARYLQSGGLLMARTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREG VPMAGYQI VQIW+AISLLTDALALAGQALLA S+TLQDY+HSRQ
Sbjct: 361 VTLTLATSMAAREGPVPMAGYQISVQIWMAISLLTDALALAGQALLAGSFTLQDYKHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480
           VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLE ARSG  FVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLETARSGSLFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540
           VDGLYYGVSDFGYAAYSMVLVGL+SS++LLVVTP FGLPGVWSGLFLFMMLRL+AGIW  
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLVSSIFLLVVTPTFGLPGVWSGLFLFMMLRLVAGIWSE 537

Query: 541 GT 543
            T
Sbjct: 541 NT 537

BLAST of CmaCh03G003240 vs. NCBI nr
Match: XP_022978135.1 (protein DETOXIFICATION 44, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1038.1 bits (2683), Expect = 2.8e-299
Identity = 559/559 (100.00%), Postives = 559/559 (100.00%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS
Sbjct: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480
           VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV
Sbjct: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540
           VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

Query: 541 GTKSGPWEMVFNELDEKSD 560
           GTKSGPWEMVFNELDEKSD
Sbjct: 541 GTKSGPWEMVFNELDEKSD 559

BLAST of CmaCh03G003240 vs. NCBI nr
Match: XP_022949803.1 (protein DETOXIFICATION 44, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1020.0 bits (2636), Expect = 7.8e-294
Identity = 548/559 (98.03%), Postives = 553/559 (98.93%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASSEQLRRNGPGKKSSNSLLS  VSVPLLNRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH N
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKNT QINIDGIEENQGKKLLSSVSTSLALAAG+GIAEAVMLSLGSGSLMDIMGIPVGSS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEIS TLSSIDGGR+ARYLKSGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540
           VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

Query: 541 GTKSGPWEMVFNELDEKSD 560
           GTKSGPWEMVFNELDEKSD
Sbjct: 541 GTKSGPWEMVFNELDEKSD 559

BLAST of CmaCh03G003240 vs. NCBI nr
Match: XP_023543949.1 (protein DETOXIFICATION 44, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023543950.1 protein DETOXIFICATION 44, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023543951.1 protein DETOXIFICATION 44, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1016.5 bits (2627), Expect = 8.6e-293
Identity = 548/559 (98.03%), Postives = 552/559 (98.75%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIYVP FNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK
Sbjct: 1   MATGLSIYVPSFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASSEQLRRNGPGKKSSNSLLS  VSVPLLNRFRDAVFKFDKLALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH N
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKNT QINIDGIEENQGKKLLSSVSTSLALAAG+GIAEAVMLSLGSGSLMDIMGIPVGSS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEIS TLSSIDGGRIARYLKSGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRIARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREGTV MAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVSMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540
           VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

Query: 541 GTKSGPWEMVFNELDEKSD 560
           GTKSGPWEMVFN+LDEKSD
Sbjct: 541 GTKSGPWEMVFNDLDEKSD 559

BLAST of CmaCh03G003240 vs. NCBI nr
Match: KAG6603561.1 (Protein DETOXIFICATION 44, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 981.9 bits (2537), Expect = 2.3e-282
Identity = 532/544 (97.79%), Postives = 537/544 (98.71%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIYVPCFNTGTNLSSK QLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKRQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASSEQLRRNGPGKKSSNSLLS  VSVPLLNRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH N
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKNT QINIDGIEENQGKKLLSSVSTSLALAAG+GIAEAVMLSLGSGSLMDIMGIPVGSS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEIS TLSSIDGGR+ARYLKSGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFV 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPV+ALAFV
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVNALAFV 480

Query: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540
           VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL
Sbjct: 481 VDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRL 540

Query: 541 GTKS 545
           GTKS
Sbjct: 541 GTKS 544

BLAST of CmaCh03G003240 vs. NCBI nr
Match: KAG7033751.1 (Protein DETOXIFICATION 44, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 964.1 bits (2491), Expect = 5.1e-277
Identity = 530/574 (92.33%), Postives = 535/574 (93.21%), Query Frame = 0

Query: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60
           MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK
Sbjct: 1   MATGLSIYVPCFNTGTNLSSKCQLLRRNANCQVRFRYFRKASFQKNLTTSSLKSPPEEPK 60

Query: 61  STASSEQLRRNGPGKKSSNSLLSTFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALA 120
           STASSEQLRRNGPGKKSSNSLLS  VSVPLLNRFRDA FKFD+LALDILAIALPAALALA
Sbjct: 61  STASSEQLRRNGPGKKSSNSLLSASVSVPLLNRFRDADFKFDQLALDILAIALPAALALA 120

Query: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHKN 180
           ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH N
Sbjct: 121 ADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIHTN 180

Query: 181 EKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSS 240
           EKNT QINIDGIEENQGKKLLSSVSTSLALAAG+GIAEAVMLSLGSGSLMDIMGIPVGSS
Sbjct: 181 EKNTAQINIDGIEENQGKKLLSSVSTSLALAAGLGIAEAVMLSLGSGSLMDIMGIPVGSS 240

Query: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300
           MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC
Sbjct: 241 MRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFC 300

Query: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVL 360
           GFGIGGAAIATVISEYLIAFVLLWRLNGEIS TLSSIDGGR+ARYLKSGGLLMGRTLAVL
Sbjct: 301 GFGIGGAAIATVISEYLIAFVLLWRLNGEISFTLSSIDGGRVARYLKSGGLLMGRTLAVL 360

Query: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420
           VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ
Sbjct: 361 VTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQ 420

Query: 421 VIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLW-------------- 480
           VIYRTLQIGLISGISL+IILFLGFGAFSGLFSADAEVLEIARSGLW              
Sbjct: 421 VIYRTLQIGLISGISLSIILFLGFGAFSGLFSADAEVLEIARSGLWEDPVISHDATYTHY 480

Query: 481 ----------------FVAGSQPVSALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLL 540
                           FVAGSQPV+ALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLL
Sbjct: 481 KAIVIGCPDILAMFLQFVAGSQPVNALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLL 540

Query: 541 VVTPAFGLPGVWSGLFLFMMLRLIAGIWRLGTKS 545
           VVTPAFGLPGVWSGLFLFMMLRLIAGIWR G  S
Sbjct: 541 VVTPAFGLPGVWSGLFLFMMLRLIAGIWRAGPPS 574

BLAST of CmaCh03G003240 vs. TAIR 10
Match: AT2G38330.1 (MATE efflux family protein )

HSP 1 Score: 569.7 bits (1467), Expect = 2.6e-162
Identity = 302/450 (67.11%), Postives = 371/450 (82.44%), Query Frame = 0

Query: 103 KLALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSKLFNVPL 162
           K+ ++I++IALPAALALAADPI SL+DTAFVGHIGS ELAAVGVS SVFNLVSKLFNVPL
Sbjct: 75  KIGMEIMSIALPAALALAADPITSLVDTAFVGHIGSAELAAVGVSVSVFNLVSKLFNVPL 134

Query: 163 LNITTSFVAEEQALIHKNEKNTVQINIDGIEENQGKKLLSSVSTSLALAAGIGIAEAVML 222
           LN+TTSFVAEEQA+  K++ ++++ +         KK+L SVSTSL LAAG+GIAEA+ L
Sbjct: 135 LNVTTSFVAEEQAIAAKDDNDSIETS---------KKVLPSVSTSLVLAAGVGIAEAIAL 194

Query: 223 SLGSGSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYAT 282
           SLGS  LMD+M IP  S MR PAEQFL LRA+GAPPIV+ALAAQG FRGFKDT TPLYA 
Sbjct: 195 SLGSDFLMDVMAIPFDSPMRIPAEQFLRLRAYGAPPIVVALAAQGAFRGFKDTTTPLYAV 254

Query: 283 AAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRI 342
            AGN+LNAVLDP+LIF  GFGI GAA ATVISEYLIAF+LLW+LN  + L    I  GR 
Sbjct: 255 VAGNVLNAVLDPILIFVLGFGISGAAAATVISEYLIAFILLWKLNENVVLLSPQIKVGRA 314

Query: 343 ARYLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAG 402
            +YLKSGGLL+GRT+A+LV  TLATS+AA+ G   MAG+QI ++IWLA+SLLTDALA+A 
Sbjct: 315 NQYLKSGGLLIGRTVALLVPFTLATSLAAQNGPTQMAGHQIVLEIWLAVSLLTDALAIAA 374

Query: 403 QALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIAR 462
           Q+LLA++Y+  +Y+ +R+V++  LQ+GL +G  LA +LF+ F  FS LF+ D+EVL+IA 
Sbjct: 375 QSLLATTYSQGEYKQAREVLFGVLQVGLATGTGLAAVLFITFEPFSSLFTTDSEVLKIAL 434

Query: 463 SGLWFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVW 522
           SG  FVAGSQPV+ALAFV+DGLYYGVSDFG+AAYSMV+VG ISSL++LV  P FGL G+W
Sbjct: 435 SGTLFVAGSQPVNALAFVLDGLYYGVSDFGFAAYSMVIVGFISSLFMLVAAPTFGLAGIW 494

Query: 523 SGLFLFMMLRLIAGIWRLGTKSGPWEMVFN 553
           +GLFLFM LRL+AG WRLGT++GPW+M+++
Sbjct: 495 TGLFLFMALRLVAGAWRLGTRTGPWKMLWS 515

BLAST of CmaCh03G003240 vs. TAIR 10
Match: AT4G38380.1 (MATE efflux family protein )

HSP 1 Score: 409.8 bits (1052), Expect = 3.4e-114
Identity = 235/469 (50.11%), Postives = 321/469 (68.44%), Query Frame = 0

Query: 83  STFVSVPLLNRFRDAVFKFDKLALDILAIALPAALALAADPIASLIDTAFVGHIGSTELA 142
           S+   +P +N     V +   +  +++ ++LPA    A DP+  L++TA++G +GS EL 
Sbjct: 84  SSLDKLPEVNGVHTGVARPVDIKRELVMLSLPAIAGQAIDPLTLLMETAYIGRLGSVELG 143

Query: 143 AVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH----KNEKNTVQINIDGIEENQGK 202
           + GVS ++FN +SKLFN+PLL++ TSFVAE+ A I      +E +   I   G+ E   +
Sbjct: 144 SAGVSMAIFNTISKLFNIPLLSVATSFVAEDIAKIAAQDLASEDSQSDIPSQGLPE---R 203

Query: 203 KLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPP 262
           K LSSVST+L LA GIGI EA+ LSL SG  + +MGI   S M  PA QFL LRA GAP 
Sbjct: 204 KQLSSVSTALVLAIGIGIFEALALSLASGPFLRLMGIQSMSEMFIPARQFLVLRALGAPA 263

Query: 263 IVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLI 322
            V++LA QG FRGFKDTKTP+Y    GN L   L PL I+    G+ GAAI++VIS+Y +
Sbjct: 264 YVVSLALQGIFRGFKDTKTPVYCLGIGNFLAVFLFPLFIYKFRMGVAGAAISSVISQYTV 323

Query: 323 AFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPM 382
           A ++L  LN  + L    I   +   YLKSGG ++GRTL+VLVT+T+ATSMAAR+G   M
Sbjct: 324 AILMLILLNKRVILLPPKIGSLKFGDYLKSGGFVLGRTLSVLVTMTVATSMAARQGVFAM 383

Query: 383 AGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAI 442
           A +QIC+Q+WLA+SLLTDALA +GQAL+ASS + +D+E  ++V    L+IG+++GI+LAI
Sbjct: 384 AAHQICMQVWLAVSLLTDALASSGQALIASSASKRDFEGVKEVTTFVLKIGVVTGIALAI 443

Query: 443 ILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSM 502
           +L + F + +GLFS D EVL I R G+ FVA +QP++ALAF+ DGL+YG+SDF YAA SM
Sbjct: 444 VLGMSFSSIAGLFSKDPEVLRIVRKGVLFVAATQPITALAFIFDGLHYGMSDFPYAACSM 503

Query: 503 VLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRLGTKSGPW 548
           ++VG ISS ++L      GL GVW GL +FM LR++AG  RL  + GPW
Sbjct: 504 MVVGGISSAFMLYAPAGLGLSGVWVGLSMFMGLRMVAGFSRLMWRKGPW 549

BLAST of CmaCh03G003240 vs. TAIR 10
Match: AT1G51340.2 (MATE efflux family protein )

HSP 1 Score: 381.7 bits (979), Expect = 1.0e-105
Identity = 224/511 (43.84%), Postives = 321/511 (62.82%), Query Frame = 0

Query: 77  SSNSLLSTFVSVPLLNRFRD--AVFKFDKLALDILAIALPAALALAADPIASLIDTAFVG 136
           S +   + F   PL   F D  +V KFD+L L+I  IALPAALAL ADPIASL+DTAF+G
Sbjct: 3   SEDGYNTDFPRNPLYIFFSDFRSVLKFDELGLEIARIALPAALALTADPIASLVDTAFIG 62

Query: 137 HIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQALIH-----KNEKNTVQINI 196
            IG  ELAAVGVS ++FN VS++   PL++ITTSFVAEE A        ++ K  ++I I
Sbjct: 63  QIGPVELAAVGVSIALFNQVSRIAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGI 122

Query: 197 DGIEEN------------------------------QGKKLLSSVSTSLALAAGIGIAEA 256
           +   E                                 K+ + S S++L +   +G+ +A
Sbjct: 123 NNPTEETIELIPEKHKDSLSDEFKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQA 182

Query: 257 VMLSLGSGSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPL 316
           V L   +  L+  MG+   S M  P++++LSLR+ GAP ++++LAAQG FRGFKDT TPL
Sbjct: 183 VFLISAAKPLLSFMGVKHDSPMMRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPL 242

Query: 317 YATAAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDG 376
           +AT  G++ N +LDP+ IF    G+ GAA A VIS+YL+  +LLW+L G++ +   S   
Sbjct: 243 FATVIGDVTNIILDPIFIFVFRLGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKH 302

Query: 377 GRIARYLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALA 436
            +  R++K+G LL+ R +AV   +TL+ S+AAREG+  MA +Q+C+Q+WLA SLL D  A
Sbjct: 303 LQFCRFMKNGFLLLMRVIAVTFCVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYA 362

Query: 437 LAGQALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLE 496
           +AGQA+LAS++  +DY+ +     R LQ+GL+ G  LA+IL  G    + +F+ D +VL 
Sbjct: 363 VAGQAILASAFAKKDYKRAAATASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLH 422

Query: 497 IARSGLWFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLP 551
           +   GL FVAG+QP++ALAFV DG+ +G SDFGYAA S+V+V ++S L LL ++   G  
Sbjct: 423 LISIGLPFVAGTQPINALAFVFDGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFI 482

BLAST of CmaCh03G003240 vs. TAIR 10
Match: AT1G51340.1 (MATE efflux family protein )

HSP 1 Score: 380.6 bits (976), Expect = 2.2e-105
Identity = 218/489 (44.58%), Postives = 313/489 (64.01%), Query Frame = 0

Query: 97  AVFKFDKLALDILAIALPAALALAADPIASLIDTAFVGHIGSTELAAVGVSASVFNLVSK 156
           +V KFD+L L+I  IALPAALAL ADPIASL+DTAF+G IG  ELAAVGVS ++FN VS+
Sbjct: 19  SVLKFDELGLEIARIALPAALALTADPIASLVDTAFIGQIGPVELAAVGVSIALFNQVSR 78

Query: 157 LFNVPLLNITTSFVAEEQALIH-----KNEKNTVQINIDGIEEN---------------- 216
           +   PL++ITTSFVAEE A        ++ K  ++I I+   E                 
Sbjct: 79  IAIFPLVSITTSFVAEEDACSSQQDTVRDHKECIEIGINNPTEETIELIPEKHKDSLSDE 138

Query: 217 --------------QGKKLLSSVSTSLALAAGIGIAEAVMLSLGSGSLMDIMGIPVGSSM 276
                           K+ + S S++L +   +G+ +AV L   +  L+  MG+   S M
Sbjct: 139 FKTSSSIFSISKPPAKKRNIPSASSALIIGGVLGLFQAVFLISAAKPLLSFMGVKHDSPM 198

Query: 277 RAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDTKTPLYATAAGNLLNAVLDPLLIFFCG 336
             P++++LSLR+ GAP ++++LAAQG FRGFKDT TPL+AT  G++ N +LDP+ IF   
Sbjct: 199 MRPSQRYLSLRSLGAPAVLLSLAAQGVFRGFKDTTTPLFATVIGDVTNIILDPIFIFVFR 258

Query: 337 FGIGGAAIATVISEYLIAFVLLWRLNGEISLTLSSIDGGRIARYLKSGGLLMGRTLAVLV 396
            G+ GAA A VIS+YL+  +LLW+L G++ +   S    +  R++K+G LL+ R +AV  
Sbjct: 259 LGVTGAATAHVISQYLMCGILLWKLMGQVDIFNMSTKHLQFCRFMKNGFLLLMRVIAVTF 318

Query: 397 TLTLATSMAAREGTVPMAGYQICVQIWLAISLLTDALALAGQALLASSYTLQDYEHSRQV 456
            +TL+ S+AAREG+  MA +Q+C+Q+WLA SLL D  A+AGQA+LAS++  +DY+ +   
Sbjct: 319 CVTLSASLAAREGSTSMAAFQVCLQVWLATSLLADGYAVAGQAILASAFAKKDYKRAAAT 378

Query: 457 IYRTLQIGLISGISLAIILFLGFGAFSGLFSADAEVLEIARSGLWFVAGSQPVSALAFVV 516
             R LQ+GL+ G  LA+IL  G    + +F+ D +VL +   GL FVAG+QP++ALAFV 
Sbjct: 379 ASRVLQLGLVLGFVLAVILGAGLHFGARVFTKDDKVLHLISIGLPFVAGTQPINALAFVF 438

Query: 517 DGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPAFGLPGVWSGLFLFMMLRLIAGIWRLG 551
           DG+ +G SDFGYAA S+V+V ++S L LL ++   G  G+W GL ++M LR   G WR+G
Sbjct: 439 DGVNFGASDFGYAAASLVMVAIVSILCLLFLSSTHGFIGLWFGLTIYMSLRAAVGFWRIG 498

BLAST of CmaCh03G003240 vs. TAIR 10
Match: AT3G08040.1 (MATE efflux family protein )

HSP 1 Score: 355.1 bits (910), Expect = 1.0e-97
Identity = 216/515 (41.94%), Postives = 309/515 (60.00%), Query Frame = 0

Query: 76  KSSNSLLSTFVSVPLLNRFRDA--VFKFDKLALDILAIALPAALALAADPIASLIDTAFV 135
           ++ + L +    +P L  F+D   VF  D    +IL IA PAALALAADPIASLIDTAFV
Sbjct: 3   ETGDDLATVKKPIPFLVIFKDLRHVFSRDTTGREILGIAFPAALALAADPIASLIDTAFV 62

Query: 136 GHIGSTELAAVGVSASVFNLVSKLFNVPLLNITTSFVAEEQA------------LIH--- 195
           G +G+ +LAAVGVS ++FN  S++   PL+++TTSFVAEE              L+H   
Sbjct: 63  GRLGAVQLAAVGVSIAIFNQASRITIFPLVSLTTSFVAEEDTMEKMKEEANKANLVHAET 122

Query: 196 ---------------KNEKNTVQ--------INIDGIEENQGKKLLSSVSTSLALAAGIG 255
                           N+ N  Q         N       + K+ + + ST++ L   +G
Sbjct: 123 ILVQDSLEKGISSPTSNDTNQPQQPPAPDTKSNSGNKSNKKEKRTIRTASTAMILGLILG 182

Query: 256 IAEAVMLSLGSGSLMDIMGIPVGSSMRAPAEQFLSLRAFGAPPIVIALAAQGTFRGFKDT 315
           + +A+ L   S  L+ +MG+   S M +PA ++LS+RA GAP ++++LA QG FRGFKDT
Sbjct: 183 LVQAIFLIFSSKLLLGVMGVKPNSPMLSPAHKYLSIRALGAPALLLSLAMQGIFRGFKDT 242

Query: 316 KTPLYATAAGNLLNAVLDPLLIFFCGFGIGGAAIATVISEYLIAFVLLWRLNGEISLTLS 375
           KTPL+AT   +++N VLDP+ IF    GI GAAIA VIS+Y +  +L   L  +++L   
Sbjct: 243 KTPLFATVVADVINIVLDPIFIFVLRLGIIGAAIAHVISQYFMTLILFVFLAKKVNLIPP 302

Query: 376 SIDGGRIARYLKSGGLLMGRTLAVLVTLTLATSMAAREGTVPMAGYQICVQIWLAISLLT 435
           +    +  R+LK+G LL+ RT+AV    TLA +MAAR GT PMA +QIC+Q+WL  SLL 
Sbjct: 303 NFGDLQFGRFLKNGLLLLARTIAVTFCQTLAAAMAARLGTTPMAAFQICLQVWLTSSLLN 362

Query: 436 DALALAGQALLASSYTLQDYEHSRQVIYRTLQIGLISGISLAIILFLGFGAFSGLFSADA 495
           D LA+AGQA+LA S+  +DY     V  R LQ+G + G+ L++ + LG    +G+FS D 
Sbjct: 363 DGLAVAGQAILACSFAEKDYNKVTAVASRVLQMGFVLGLGLSVFVGLGLYFGAGVFSKDP 422

Query: 496 EVLEIARSGLWFVAGSQPVSALAFVVDGLYYGVSDFGYAAYSMVLVGLISSLYLLVVTPA 551
            V+ +   G+ F+A +QP+++LAFV+DG+ +G SDF Y AYSMV V  IS   ++ +   
Sbjct: 423 AVIHLMAIGIPFIAATQPINSLAFVLDGVNFGASDFAYTAYSMVGVAAISIAAVIYMAKT 482