CmaCh02G015420 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh02G015420
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionvesicle-associated protein 1-2-like
LocationCma_Chr02: 8757487 .. 8761017 (+)
RNA-Seq ExpressionCmaCh02G015420
SyntenyCmaCh02G015420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACGTGTTTTCCTCCACCCTCTCTTGTTCGCAACACGTTCGCCATTGTTGCCCCTCTCTTTTCTCGATCTGTTCGAAACTTGCTCTCAAGCAACAAGCTCATTTTCCAGATTCTTTTTTCTTTCTATAACCTCTTCAATTTCGTCTTTTTCCCCTTTTGTTTGTTCTTCACTCTTCCAACAATGAGTACGGGAGAACTCCTCAGCATCGAACCTCTCGAGCTCAAGTTTGTCTGTGAGTAGTTGTTGATTCTAGAGGGACTGATTTTCTTCTTGCGGATCTGTAATATTTTGAGGGATCTTTTTTGTTTTTTTGTTTTCGTTTCTTTGGTGCTTAATTTGTTTCTGTTTGTCTGTTTTTGGTCAGTCGAATTGAAGAAGCAGATCTCTTGTTCCCTTCAATTGTCTAACAAGACCGAGAGCTACGTTGCATTTAAGGTCTGTGTTTCGTTTCTCTCTATTTGAATCTTTTTGGGTTTTGATTCTATTTTTGTGATTTGTGTAATTGATCTTTTTTCGAGTGAAATGGTTTGGTGCTTGTAGTATGGAAGTGTTTTGGATTTTGAATTTAGGCGTGTGCACTTTTTTGTTGTTGTTGTTGTTTCTTTTGCTTTTTTTTCTTGATGTATCTGTCTGGTGCCAGATCACGCCCTGAAACTAGTTAATTTCGTCATTTTTCAATTTGCTTGCGGGTGTTTTGGTGTTTATAATTCATTTTTTATTGGCAAATTGAGCGAAGATTAAAGCTCGTATGATTACTTTGTCGACTTAGTATGAAGTTTATCTCTTTCTAGGTGAAAACTACGAACCCTAAAAAGTACTGCGTTCGCCCCAACACTGGAGTTGTCTCGCCAGGGTCAACATGCGATGTTATAGGTGCCAAATGCCAATATCAAATATTTTCCTTCTTTGATTTTAGTTTTCAGATGAGTTTTGTGGTTATTGAAATGGGTAGTACTTTGTTTTGTAGTCACCATGCAAGCCCAGAAAGAAGCTCCTGCTGATATGCAATGCAAAGACAAGTTCCTCCTTCAGAGTGTTAAAACCTTCGATGGCGCAACCGCAAAGGATATCAATGCAGAAATGGTATTTATACTGTGAATGTTCGAAGTTTGTAGTTAATTTTAGCACCTTACTTTTGTTTATGGGCGTGGGGTTTGCAGTTCAACAAGGAGGCTGGGCATGTGGTTGAGGAATGCAAATTGAAAGTCGTTTATTCTCCTCCAGCTCAGCCACCATCTCCAGTACCAGAAGGTTCTGAAGAAGGATCCTCTCCAAGAGGTTCCATTTCAGAGAATGGAAACTTCAATGATACCGAATTAAACACTGTGAGTAGTCGAGTGTTGGGTTTTCTTTTCCCTTTTCTTTCATCTGCGCATTTAATTTATACATCTTCACCTTCTGTTTGCAGGCTACAAGGGGATATGTTGATCGGTCGGAGCCCCAGGATAAATCTGCAGAGGTAAAAAAGGGTGATTATTGGCTTGTAATAGGCTTAAAGCTTATGTCTGCAGTTTACTGTGATTCTCTCTTTTGTTTTCTGCAACTCTGTGGATGTGTTCAAAAAAATTTCTTCCAAACTGCCACCTCTATTTTAAAATTTTTATAAGATAGAACTCAATGCTAAATTATGCCCTTGTGATTTGAGTGTTACTCTGAGTAGTAACAATATAATTTGCTTTGGGCAGTATAATAATTGATAATACACTCCGTGAAGTTTTGGGTGAGCTTGTTGTTTCCTGTTCTTGTTCTTGGGGCTGTTCACTTTCATTTGGTCTATATTTTAAGCATCTTTTTTGTATTTTTAACCTTTACTGAATGCATTGAAATGCACAATTGGAGTTCTTAGTGTTAACCACTTAACATACACATGGATGTAACTTCCTTGGCATAATATGCGAATGGTTGAGGATTTACTTTCAGAATTTTGTTTAAGACTGCTGAGCACATGTTTTGTATAGATTGTTTTTATAATGGTTAGGTTTCTTATGTGATCTTTGATCATTTTCCTTAACTTTAGTGGAAACCACTTGATAACATGATAGAAGTATTTTCATCACAAAATAATAAATTTAGAGAAGTACTGCTATAGTTAATCTCCGAAAGAACATACACTGTGGAGTAGATATAATCTCTTGTCCCCAAGTATTGCAATACTATCCCACCATGAGTTGACGGTTGGCTTGGCAGGGAAGCGTTTAACTGTAAACTTGGATATCTTTGAGGTCGTAAATTCAAGCCATGAGTGTCACCTACCTTGGGTATTAAATATTTTACCTTTTTTCCAGTGCCAAATAAAGAGTTGTGGTAGGATACATCCACTTGGAGAGAACCTCCCTAAACTGTTAGACTTCAACAATCCCTTAAATATATATATACACGCACGCACACGCACACACAAGGATGATGCTATATTCTTGTCTTGTTAATGTGCATTAAACTCTTATAGGTGTCTGCCAATATATTAGGCTTAAGATATTGGAGTTAATATGAGAATCTCTATCCCTTTTGCTTCGGAACTCTTTGTCAAAATCTAAAAAGTACCATTATTCATAGGTCCAACCACCTTTGGATCCCCTGTTCTCTCTTTTTTTTTAACCCTTCTGTTTGTGCAATGTTCTCAAACCTTTATCGTGGTTCTCTGTGATTTTTAAACCATTCAGCACCGCACAGATGGTTTGATTGACTAAATCATGGCCACTGGAGCTATTTGTGTACATGTGTTTGTGTTTTTCGTTTCTGCGTATGCTGTACTCTCAGCTTGTAGCAGTCTGCTTATTGGCTACTTTAACATGTGCAGGCTAGAGCCCTCATAGCTAAACTTACAGAGGAAAAGAATAGTGCCCTCCAACAGAATAATAAACTTCGTCAGGAATTGGTAAGAGGGGTTTTATTACATGGGAAACATTGTCTTCACCATTGTGTTATACTTGAATAAATCTCCAAGTCTCTTTACCTGAATACCTTTTTGAAGAATTACCCAAATAGAATATTTACTATTCTGAACTTTGTTTGTAAGGTTGCAAATCAGGCTATGCTCAAATCTGATTCTTTTCTTGGAATACATTAGTTTCTGATGTGTTGGAGGTTTGAATTGTGCAGGAACTTTTGAAGCGCGATGGCAGGAAAAATGGCGGTGGTGTCTCCTTCCTGCTTGTCATATTAATTGGATTGATCGGAATCATGTTGGGCTATATTATTAAGAAGACTTGACAAACTCATGGCAGCAAAACTGGATTTATATCATTATATATTCTATGATGAAAGGAAGGGGGAACTACTAATGTTCAGTGGGTGGATCTGTAGCGAGTTTTAATTTGTTGGAAGTAGCAAATTGTTAGATGGTGTTCAAGGATTTGTAGTTACTGAATTTGCAAGGTAGCACACTCTTTCTGTAACTTAAAAGAGTTTTCTTTTTTTCCCTATTTTTTTTTTCTCTATTTTTTCTTCTCTGCTTCTCTTATCAACCAACTTGTTATATTTTGTGAAACTGTTGATTTAATAATTTTACATCCTCAATTGTTCAGCCATTT

mRNA sequence

CACGTGTTTTCCTCCACCCTCTCTTGTTCGCAACACGTTCGCCATTGTTGCCCCTCTCTTTTCTCGATCTGTTCGAAACTTGCTCTCAAGCAACAAGCTCATTTTCCAGATTCTTTTTTCTTTCTATAACCTCTTCAATTTCGTCTTTTTCCCCTTTTGTTTGTTCTTCACTCTTCCAACAATGAGTACGGGAGAACTCCTCAGCATCGAACCTCTCGAGCTCAAGTTTGTCTTCGAATTGAAGAAGCAGATCTCTTGTTCCCTTCAATTGTCTAACAAGACCGAGAGCTACGTTGCATTTAAGGTGAAAACTACGAACCCTAAAAAGTACTGCGTTCGCCCCAACACTGGAGTTGTCTCGCCAGGGTCAACATGCGATGTTATAGTCACCATGCAAGCCCAGAAAGAAGCTCCTGCTGATATGCAATGCAAAGACAAGTTCCTCCTTCAGAGTGTTAAAACCTTCGATGGCGCAACCGCAAAGGATATCAATGCAGAAATGTTCAACAAGGAGGCTGGGCATGTGGTTGAGGAATGCAAATTGAAAGTCGTTTATTCTCCTCCAGCTCAGCCACCATCTCCAGTACCAGAAGGTTCTGAAGAAGGATCCTCTCCAAGAGGTTCCATTTCAGAGAATGGAAACTTCAATGATACCGAATTAAACACTGCTACAAGGGGATATGTTGATCGGTCGGAGCCCCAGGATAAATCTGCAGAGGCTAGAGCCCTCATAGCTAAACTTACAGAGGAAAAGAATAGTGCCCTCCAACAGAATAATAAACTTCGTCAGGAATTGGAACTTTTGAAGCGCGATGGCAGGAAAAATGGCGGTGGTGTCTCCTTCCTGCTTGTCATATTAATTGGATTGATCGGAATCATGTTGGGCTATATTATTAAGAAGACTTGACAAACTCATGGCAGCAAAACTGGATTTATATCATTATATATTCTATGATGAAAGGAAGGGGGAACTACTAATGTTCAGTGGGTGGATCTGTAGCGAGTTTTAATTTGTTGGAAGTAGCAAATTGTTAGATGGTGTTCAAGGATTTGTAGTTACTGAATTTGCAAGGTAGCACACTCTTTCTGTAACTTAAAAGAGTTTTCTTTTTTTCCCTATTTTTTTTTTCTCTATTTTTTCTTCTCTGCTTCTCTTATCAACCAACTTGTTATATTTTGTGAAACTGTTGATTTAATAATTTTACATCCTCAATTGTTCAGCCATTT

Coding sequence (CDS)

ATGAGTACGGGAGAACTCCTCAGCATCGAACCTCTCGAGCTCAAGTTTGTCTTCGAATTGAAGAAGCAGATCTCTTGTTCCCTTCAATTGTCTAACAAGACCGAGAGCTACGTTGCATTTAAGGTGAAAACTACGAACCCTAAAAAGTACTGCGTTCGCCCCAACACTGGAGTTGTCTCGCCAGGGTCAACATGCGATGTTATAGTCACCATGCAAGCCCAGAAAGAAGCTCCTGCTGATATGCAATGCAAAGACAAGTTCCTCCTTCAGAGTGTTAAAACCTTCGATGGCGCAACCGCAAAGGATATCAATGCAGAAATGTTCAACAAGGAGGCTGGGCATGTGGTTGAGGAATGCAAATTGAAAGTCGTTTATTCTCCTCCAGCTCAGCCACCATCTCCAGTACCAGAAGGTTCTGAAGAAGGATCCTCTCCAAGAGGTTCCATTTCAGAGAATGGAAACTTCAATGATACCGAATTAAACACTGCTACAAGGGGATATGTTGATCGGTCGGAGCCCCAGGATAAATCTGCAGAGGCTAGAGCCCTCATAGCTAAACTTACAGAGGAAAAGAATAGTGCCCTCCAACAGAATAATAAACTTCGTCAGGAATTGGAACTTTTGAAGCGCGATGGCAGGAAAAATGGCGGTGGTGTCTCCTTCCTGCTTGTCATATTAATTGGATTGATCGGAATCATGTTGGGCTATATTATTAAGAAGACTTGA

Protein sequence

MSTGELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVSPGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECKLKVVYSPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVDRSEPQDKSAEARALIAKLTEEKNSALQQNNKLRQELELLKRDGRKNGGGVSFLLVILIGLIGIMLGYIIKKT
Homology
BLAST of CmaCh02G015420 vs. ExPASy Swiss-Prot
Match: Q9SHC8 (Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=1)

HSP 1 Score: 301.2 bits (770), Expect = 1.0e-80
Identity = 159/240 (66.25%), Postives = 195/240 (81.25%), Query Frame = 0

Query: 3   TGELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVSPG 62
           + ELL+I+P++L+F FELKKQISCSL L NKT++YVAFKVKTTNPKKYCVRPNTGVV P 
Sbjct: 2   SNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPR 61

Query: 63  STCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECKLK 122
           S+ +V+VTMQAQKEAPAD+QCKDKFLLQ V    GAT KD+  EMF+KEAGH VEE KL+
Sbjct: 62  SSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKLR 121

Query: 123 VVYSPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVDRSEPQDKSAEARA 182
           VVY  P +PPSPV EGSEEGSSPR S+S+NGN +D     A R   DR + QD S+EARA
Sbjct: 122 VVYVAPPRPPSPVREGSEEGSSPRASVSDNGNASD--FTAAPRFSADRVDAQDNSSEARA 181

Query: 183 LIAKLTEEKNSALQQNNKLRQELELLKRDG-RKNGGGVSFLLVILIGLIGIMLGYIIKKT 242
           L+ KLTEEKNSA+Q NN+L+QEL+ L+R+  R   GG+ F+ V+L+GLIG++LGYI+K+T
Sbjct: 182 LVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMKRT 239

BLAST of CmaCh02G015420 vs. ExPASy Swiss-Prot
Match: Q8VZ95 (Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=1)

HSP 1 Score: 290.0 bits (741), Expect = 2.4e-77
Identity = 155/243 (63.79%), Postives = 197/243 (81.07%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVS 60
           MS  ELL++EPL+L+F FELKKQISCSL L+NKT++ VAFKVKTTNPKKYCVRPNTGVV 
Sbjct: 17  MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVL 76

Query: 61  PGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECK 120
           P STC+V+VTMQAQKEAP+DMQCKDKFLLQ V    G TAK++  EMF+KEAGH VEE K
Sbjct: 77  PRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETK 136

Query: 121 LKVVYSPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVD-RSEPQDKSAE 180
           L+V Y  P +PPSPV EGSEEGSSPR S+S+NG+ ++       R  VD ++  Q+ ++E
Sbjct: 137 LRVTYVAPPRPPSPVHEGSEEGSSPRASVSDNGHGSEFSFE---RFIVDNKAGHQENTSE 196

Query: 181 ARALIAKLTEEKNSALQQNNKLRQELELLKRDGRKN-GGGVSFLLVILIGLIGIMLGYII 240
           ARALI KLTEEK SA+Q NN+L++EL+ L+R+ +K+  GG+ F+ V+L+GLIG++LGYI+
Sbjct: 197 ARALITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIM 256

Query: 241 KKT 242
           K+T
Sbjct: 257 KRT 256

BLAST of CmaCh02G015420 vs. ExPASy Swiss-Prot
Match: Q84WW5 (Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 2.8e-70
Identity = 144/242 (59.50%), Postives = 192/242 (79.34%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFVFELKKQISCSLQLSNKTESY-VAFKVKTTNPKKYCVRPNTGVV 60
           M+TG+L++I P ELKF FELKKQ SCS+QL+NKT +  VAFKVKTTNP+KYCVRPNTGVV
Sbjct: 1   MTTGDLVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVV 60

Query: 61  SPGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEEC 120
            PG +C+V VTMQAQKEAP DMQCKDKFL+Q+V   DG T+K++ AEMFNKEAG V+E+ 
Sbjct: 61  LPGDSCNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDF 120

Query: 121 KLKVVYSPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVDRSEPQDKSAE 180
           KL+VVY  PA PPSPVPEGSEEG+SP  S+++  + + +  +  +R + + SE   KS+E
Sbjct: 121 KLRVVYI-PANPPSPVPEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSE---KSSE 180

Query: 181 ARALIAKLTEEKNSALQQNNKLRQELELLKRD-GRKNGGGVSFLLVILIGLIGIMLGYII 240
           A ++I+KLTEEK SA QQ+ KLR ELE+L+++  +K  GG S LL++L+GL+G ++GY++
Sbjct: 181 AWSMISKLTEEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLL 238

BLAST of CmaCh02G015420 vs. ExPASy Swiss-Prot
Match: Q9SYC9 (Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 3.1e-48
Identity = 97/153 (63.40%), Postives = 120/153 (78.43%), Query Frame = 0

Query: 5   ELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVSPGST 64
           ELL I+P++++F  EL K++SCSL L+NKTE+YVAFK KTTN KKY VRPN GVV P S+
Sbjct: 175 ELLIIDPVDVQFPIELNKKVSCSLNLTNKTENYVAFKAKTTNAKKYYVRPNVGVVLPRSS 234

Query: 65  CDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECKLKVV 124
           C+V+V MQA KEAPADMQC+DK L Q        TAKD+ +EMF+KEAGH  EE +LKV+
Sbjct: 235 CEVLVIMQALKEAPADMQCRDKLLFQCKVVEPETTAKDVTSEMFSKEAGHPAEETRLKVM 294

Query: 125 YSPPAQPPSPVPEGSEEGSSPRGSISENGNFND 158
           Y  P QPPSPV EG+EEGSSPR S+S+NGN ++
Sbjct: 295 YVTPPQPPSPVQEGTEEGSSPRASVSDNGNASE 327

BLAST of CmaCh02G015420 vs. ExPASy Swiss-Prot
Match: Q9LVU1 (Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=1)

HSP 1 Score: 173.7 bits (439), Expect = 2.5e-42
Identity = 104/239 (43.51%), Postives = 148/239 (61.92%), Query Frame = 0

Query: 5   ELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVSPGST 64
           +L+SI+P ELKF+FEL+KQ  C L+++NKTE+YVAFKVKTT+PKKY VRPNTGV+ P  +
Sbjct: 8   QLISIQPDELKFLFELEKQSYCDLKVANKTENYVAFKVKTTSPKKYFVRPNTGVIQPWDS 67

Query: 65  CDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECKLKVV 124
           C + VT+QAQ+E P DMQCKDKFLLQS          ++  + F K++G  + ECKLKV 
Sbjct: 68  CIIRVTLQAQREYPPDMQCKDKFLLQSTIVPPHTDVDELPQDTFTKDSGKTLTECKLKVS 127

Query: 125 YSPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVDRSEPQDKSAEARALI 184
           Y      PS     SE G                    AT G         +S+E  + I
Sbjct: 128 YI----TPSTTQRSSESG--------------------ATNG-------DGQSSETISTI 187

Query: 185 AKLTEEKNSALQQNNKLRQELELL--KRDGRKNGGGVSFLLVILIGLIGIMLGYIIKKT 242
            +L EE+++A++Q  +L+ ELE +  +R+ R +G G+S  L  ++GLIG+++G+I+K T
Sbjct: 188 QRLKEERDAAVKQTQQLQHELETVRRRRNQRNSGNGLSLKLAAMVGLIGLIIGFILKLT 215

BLAST of CmaCh02G015420 vs. TAIR 10
Match: AT2G45140.1 (plant VAP homolog 12 )

HSP 1 Score: 301.2 bits (770), Expect = 7.4e-82
Identity = 159/240 (66.25%), Postives = 195/240 (81.25%), Query Frame = 0

Query: 3   TGELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVSPG 62
           + ELL+I+P++L+F FELKKQISCSL L NKT++YVAFKVKTTNPKKYCVRPNTGVV P 
Sbjct: 2   SNELLTIDPVDLQFPFELKKQISCSLYLGNKTDNYVAFKVKTTNPKKYCVRPNTGVVHPR 61

Query: 63  STCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECKLK 122
           S+ +V+VTMQAQKEAPAD+QCKDKFLLQ V    GAT KD+  EMF+KEAGH VEE KL+
Sbjct: 62  SSSEVLVTMQAQKEAPADLQCKDKFLLQCVVASPGATPKDVTHEMFSKEAGHRVEETKLR 121

Query: 123 VVYSPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVDRSEPQDKSAEARA 182
           VVY  P +PPSPV EGSEEGSSPR S+S+NGN +D     A R   DR + QD S+EARA
Sbjct: 122 VVYVAPPRPPSPVREGSEEGSSPRASVSDNGNASD--FTAAPRFSADRVDAQDNSSEARA 181

Query: 183 LIAKLTEEKNSALQQNNKLRQELELLKRDG-RKNGGGVSFLLVILIGLIGIMLGYIIKKT 242
           L+ KLTEEKNSA+Q NN+L+QEL+ L+R+  R   GG+ F+ V+L+GLIG++LGYI+K+T
Sbjct: 182 LVTKLTEEKNSAVQLNNRLQQELDQLRRESKRSKSGGIPFMYVLLVGLIGLILGYIMKRT 239

BLAST of CmaCh02G015420 vs. TAIR 10
Match: AT3G60600.1 (vesicle associated protein )

HSP 1 Score: 290.0 bits (741), Expect = 1.7e-78
Identity = 155/243 (63.79%), Postives = 197/243 (81.07%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVS 60
           MS  ELL++EPL+L+F FELKKQISCSL L+NKT++ VAFKVKTTNPKKYCVRPNTGVV 
Sbjct: 17  MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVL 76

Query: 61  PGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECK 120
           P STC+V+VTMQAQKEAP+DMQCKDKFLLQ V    G TAK++  EMF+KEAGH VEE K
Sbjct: 77  PRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETK 136

Query: 121 LKVVYSPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVD-RSEPQDKSAE 180
           L+V Y  P +PPSPV EGSEEGSSPR S+S+NG+ ++       R  VD ++  Q+ ++E
Sbjct: 137 LRVTYVAPPRPPSPVHEGSEEGSSPRASVSDNGHGSEFSFE---RFIVDNKAGHQENTSE 196

Query: 181 ARALIAKLTEEKNSALQQNNKLRQELELLKRDGRKN-GGGVSFLLVILIGLIGIMLGYII 240
           ARALI KLTEEK SA+Q NN+L++EL+ L+R+ +K+  GG+ F+ V+L+GLIG++LGYI+
Sbjct: 197 ARALITKLTEEKQSAIQLNNRLQRELDQLRRESKKSQSGGIPFMYVLLVGLIGLILGYIM 256

Query: 241 KKT 242
           K+T
Sbjct: 257 KRT 256

BLAST of CmaCh02G015420 vs. TAIR 10
Match: AT4G00170.1 (Plant VAMP (vesicle-associated membrane protein) family protein )

HSP 1 Score: 266.5 bits (680), Expect = 2.0e-71
Identity = 144/242 (59.50%), Postives = 192/242 (79.34%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFVFELKKQISCSLQLSNKTESY-VAFKVKTTNPKKYCVRPNTGVV 60
           M+TG+L++I P ELKF FELKKQ SCS+QL+NKT +  VAFKVKTTNP+KYCVRPNTGVV
Sbjct: 1   MTTGDLVNIHPTELKFPFELKKQSSCSMQLTNKTTTQCVAFKVKTTNPRKYCVRPNTGVV 60

Query: 61  SPGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEEC 120
            PG +C+V VTMQAQKEAP DMQCKDKFL+Q+V   DG T+K++ AEMFNKEAG V+E+ 
Sbjct: 61  LPGDSCNVTVTMQAQKEAPLDMQCKDKFLVQTVVVSDGTTSKEVLAEMFNKEAGRVIEDF 120

Query: 121 KLKVVYSPPAQPPSPVPEGSEEGSSPRGSISENGNFNDTELNTATRGYVDRSEPQDKSAE 180
           KL+VVY  PA PPSPVPEGSEEG+SP  S+++  + + +  +  +R + + SE   KS+E
Sbjct: 121 KLRVVYI-PANPPSPVPEGSEEGNSPMASLNDIASQSASLFDDVSRTFEETSE---KSSE 180

Query: 181 ARALIAKLTEEKNSALQQNNKLRQELELLKRD-GRKNGGGVSFLLVILIGLIGIMLGYII 240
           A ++I+KLTEEK SA QQ+ KLR ELE+L+++  +K  GG S LL++L+GL+G ++GY++
Sbjct: 181 AWSMISKLTEEKTSATQQSQKLRLELEMLRKETSKKQSGGHSLLLMLLVGLLGCVIGYLL 238

BLAST of CmaCh02G015420 vs. TAIR 10
Match: AT3G60600.2 (vesicle associated protein )

HSP 1 Score: 225.7 bits (574), Expect = 3.9e-59
Identity = 113/154 (73.38%), Postives = 131/154 (85.06%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVS 60
           MS  ELL++EPL+L+F FELKKQISCSL L+NKT++ VAFKVKTTNPKKYCVRPNTGVV 
Sbjct: 17  MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVL 76

Query: 61  PGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECK 120
           P STC+V+VTMQAQKEAP+DMQCKDKFLLQ V    G TAK++  EMF+KEAGH VEE K
Sbjct: 77  PRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETK 136

Query: 121 LKVVYSPPAQPPSPVPEGSEEGSSPRGSISENGN 155
           L+V Y  P +PPSPV EGSEEGSSPR S+S+NG+
Sbjct: 137 LRVTYVAPPRPPSPVHEGSEEGSSPRASVSDNGH 170

BLAST of CmaCh02G015420 vs. TAIR 10
Match: AT3G60600.3 (vesicle associated protein )

HSP 1 Score: 224.9 bits (572), Expect = 6.7e-59
Identity = 118/175 (67.43%), Postives = 138/175 (78.86%), Query Frame = 0

Query: 1   MSTGELLSIEPLELKFVFELKKQISCSLQLSNKTESYVAFKVKTTNPKKYCVRPNTGVVS 60
           MS  ELL++EPL+L+F FELKKQISCSL L+NKT++ VAFKVKTTNPKKYCVRPNTGVV 
Sbjct: 17  MSNSELLTVEPLDLQFPFELKKQISCSLYLTNKTDNNVAFKVKTTNPKKYCVRPNTGVVL 76

Query: 61  PGSTCDVIVTMQAQKEAPADMQCKDKFLLQSVKTFDGATAKDINAEMFNKEAGHVVEECK 120
           P STC+V+VTMQAQKEAP+DMQCKDKFLLQ V    G TAK++  EMF+KEAGH VEE K
Sbjct: 77  PRSTCEVLVTMQAQKEAPSDMQCKDKFLLQGVIASPGVTAKEVTPEMFSKEAGHRVEETK 136

Query: 121 LKVVYSPPAQPPSPVPEGSEEGSSPRGSISENG----------NFNDTELNTATR 166
           L+V Y  P +PPSPV EGSEEGSSPR S+S+NG          NF+  +L+  TR
Sbjct: 137 LRVTYVAPPRPPSPVHEGSEEGSSPRASVSDNGHGSEFSIFMFNFSLRDLSWTTR 191

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SHC81.0e-8066.25Vesicle-associated protein 1-2 OS=Arabidopsis thaliana OX=3702 GN=PVA12 PE=1 SV=... [more]
Q8VZ952.4e-7763.79Vesicle-associated protein 1-1 OS=Arabidopsis thaliana OX=3702 GN=PVA11 PE=1 SV=... [more]
Q84WW52.8e-7059.50Vesicle-associated protein 1-3 OS=Arabidopsis thaliana OX=3702 GN=PVA13 PE=2 SV=... [more]
Q9SYC93.1e-4863.40Vesicle-associated protein 1-4 OS=Arabidopsis thaliana OX=3702 GN=PVA14 PE=2 SV=... [more]
Q9LVU12.5e-4243.51Vesicle-associated protein 2-1 OS=Arabidopsis thaliana OX=3702 GN=PVA21 PE=1 SV=... [more]
Match NameE-valueIdentityDescription
AT2G45140.17.4e-8266.25plant VAP homolog 12 [more]
AT3G60600.11.7e-7863.79vesicle associated protein [more]
AT4G00170.12.0e-7159.50Plant VAMP (vesicle-associated membrane protein) family protein [more]
AT3G60600.23.9e-5973.38vesicle associated protein [more]
AT3G60600.36.7e-5967.43vesicle associated protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 177..211
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 143..160
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 127..160
NoneNo IPR availablePANTHERPTHR10809:SF130VESICLE-ASSOCIATED PROTEIN 1-2-LIKEcoord: 1..239
IPR013783Immunoglobulin-like foldGENE3D2.60.40.10Immunoglobulinscoord: 2..130
e-value: 1.1E-45
score: 156.7
IPR000535Major sperm protein (MSP) domainPFAMPF00635Motile_Spermcoord: 7..110
e-value: 9.2E-31
score: 105.9
IPR000535Major sperm protein (MSP) domainPROSITEPS50202MSPcoord: 6..126
score: 30.143114
IPR016763Vesicle-associated membrane-protein-associated proteinPIRSFPIRSF019693VAMP_assoc_protcoord: 1..240
e-value: 9.2E-67
score: 223.9
IPR016763Vesicle-associated membrane-protein-associated proteinPANTHERPTHR10809VESICLE-ASSOCIATED MEMBRANE PROTEIN-ASSOCIATED PROTEINcoord: 1..239
IPR008962PapD-like superfamilySUPERFAMILY49354PapD-likecoord: 2..141

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh02G015420.1CmaCh02G015420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0090158 endoplasmic reticulum membrane organization
biological_process GO:0061817 endoplasmic reticulum-plasma membrane tethering
cellular_component GO:0005789 endoplasmic reticulum membrane
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane