CmUC11G217210 (gene) Watermelon (USVL531) v1

Overview
NameCmUC11G217210
Typegene
OrganismCitrullus mucosospermus (Watermelon (USVL531) v1)
Description4-coumarate--CoA ligase-like 1
LocationCmU531Chr11: 25567794 .. 25571101 (+)
RNA-Seq ExpressionCmUC11G217210
SyntenyCmUC11G217210
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGTTTGTATCGTGTTCACCTGTAATTTCCTTCGCAAATGGGCTTTTTCAGGTTAAAGCTGTACGAAGAATGTATAGCAGAAGAACGAAGCCTTGACCAGCGTAATTTACAATCAACTTTGGCCGGAATTTGTGAAGAAGATGGGTTCATTCGGTAAGAGTGGAAATTTAAATTTAAATTCAAATTTCTTTTTCAGAAATAGAGTCAATATCTAACGATTTTCCTTTTACGTTAAATGATATAACCCATTTTGAATAATGTTAAAATATTACGTCAAATTTCCATGAATGAAGGATCTCTCTCTGGATAGGGAAAGAAAAGGAAAGGAAAGAACTGGGAAGTATTCTCCAAGAAAAGTCACCTTTTTATGCATGGTAACGCCAGAGTTCAAGAGAGGTACATAAATGAGTCGAGATCACATCCGAAAAAGAGAGATGTGGAAATATGAAATTATGGTTGAAAAAAACTTAAAAGAATTAATAGCTACTACCTATACAAAAAAGGTGTACTTTCCTTTTTAGTAGTTCAATCATAGGAATTCTAAAGTCAAACGTGCTTAACTTAAAGCATGTGAGTGAGGACAAAACATTTTGAAAGTACCTGTGTTAGTTTGTGGGGATAGTTTTCACTCTTAGAAATAGTTTAAGACAAGCAAAGGTTATGTTCTTAACTGTAATCAGTAGATGAGAAATAATATAGATAAAAAAATGTTATAATAAATGGAGTTCTTCTCCAATGAAGTTTTATTTGGTTCAATTAATTTTAGAGCCAAGCTAGCTTCTGTTTAGAAAGAGAAAAACATGTTCCAACGTTGTTTAGAAAGAGAGCTTATAGGCCTGCCTGAATGAATGGCATGAATCAACAACATCCCACCAAAAAAAAAAAAGACTCATGCCAATCTCACCCTCAAGAACTCTACAGATTTCTCATTCACAAAGCATGATACAAGCTTTCACAACTCTTCCTTTAAGAAGAGGGAAGTCGACCCTTATCGACACTTGCAGAAAACAACAACTCCGCCATGGCTACTTGCATTCGAGATTTGGTAGAAGAAGAGGAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGATAATATCACACTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGAAAATGTGGCATTTGTGGAAGCCGTGAGTGGAAAGGCTTACACTTACCGCGAAGTTGTGAGAGACATAAATAGATTTTCCAAGGCCTTGTGCTCTCTGAGGTTAAAGAAGGGGCAGGTAGTTATTGTTGTTCTACCCAATGTTGCAGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCCGCTCATATATCAGAAATCAAAAAGCAGGTGGAGGTAGCAGAAGCCAAACTTGTCGTCACGAACAGCGCAAACTTTGAAAAGGTAAGATAGAAACTGTTGTTTATTAATCGTATTTCTTCCGAATTCAACTCGTCTCGCTTACTATCTATACTGTTGAGAACAGGTGAAGGAATTAAAGCTACCAGTGATCTTATTAGGGGAGGAACTAATTGAAGGTTCCATTAATTGGCACAAACTGCTTGAAGCTGCAGACCGTGCAGGCAACGATTGTGTTAAAGAAGATATTAAGCAGACTGATTTATGTGCCCTCCCTTTCTCATCAGGCACCACAGGGGTTTCCAAAGGCGTTATGCTAACTCACCGAAATCTAATAGCTAATTTGTGTTCGACTCTCTCCGGTGTCCCAAGAGAAATAGAGGGCAAGGTCACGACGTTAGGCCTCATTCCGTTCTTCCATATTTACGGGATCACTGGAATATGTTGTGCTGCACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACCTTCATTAATGCCCTCATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCCATCATCCTGGCTTTGGTTAAGAATCCTATTGTCGAAGAATTTGATCTTAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAAGTTCAGACTGCCTTCGAAAGAAAGTTCCCAGGCGTGGACATTCAAGAGGTAAGTTTAGTTATTACTGTGTGTAGTCAATAGATTGTGACAGATCTCTACTCTTGTTCATTCCTTCAAACTTTTTAGTTATGACATGTAGTACAACTTCCTGTTTTCGGCATATCAGAAGAAAACCGAGCTCCACCGTCACATTGCAGAGCTAATGCATTGAGTCCTAACTTGTATAATATGCTAACTAATTGTCGTTGATCACCGACAGGCATATGGACTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGTATGGGCAAAGATAATCTCACTGCAAAGAAAAACACAGTTGGCCGCATTCTTCCTAATCTAGAAGTCAAATTCATCGATCCAGACAGCGGAAGGTCTCTGCCAAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGTACGGAACGTAGTTCGGTGTAGTGTAGAAAATCTTTCTGGGATCTCTAATTAAATACCGTCGGAACTTTTCCTTCTTGTTTCAGGTTACTACAAAAATGAAGAGGAGACTTCAAGAACGATCGATAACAAAGGCTGGATGCACACTGGGGACATTGGATACATTGATGACGATGGCGACGTGTTTATTGTGGATCGTATTAAGGAATTGATCAAGTACAAAGGCTTTCAAGTAAAGACTTAACATAGCTTATTTCAAGTTGATCCATTGATTGATAAAAAGGAAGCTGAAGATGTGAAAAATGGAAGTTTGTAGATCAAACTTTTTTTTGCTTTTTTGTTTTTTTGTCTAAATGTGAACATGAAATAAGGTGATGGATGTCTTTGATTTCTTTGCAGGTTGCTCCAGCTGAGTTAGAGGCAATCCTCCTTGCTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGTAAGATTCTTCGCCTCACATTCATTTCGTTTCATGTTGTTTCAATTGAAAGATAAATTAGGTTAAAACAATGCTATGCTGTATCAGGCTGCCAGACGAAGAAGCCGGTGAGATCCCAGCAGCAAGTGTCGTGATGGCTCCGAATTCAAAAGAAACTGAAGATGAAATAATCAAACATGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTGGACACTATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGCTCGAAAAGATTCGAGCTGAATCCGCCTCTTAG

mRNA sequence

ATGGAGGGAAGTCGACCCTTATCGACACTTGCAGAAAACAACAACTCCGCCATGGCTACTTGCATTCGAGATTTGGTAGAAGAAGAGGAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGATAATATCACACTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGAAAATGTGGCATTTGTGGAAGCCGTGAGTGGAAAGGCTTACACTTACCGCGAAGTTGTGAGAGACATAAATAGATTTTCCAAGGCCTTGTGCTCTCTGAGGTTAAAGAAGGGGCAGGTAGTTATTGTTGTTCTACCCAATGTTGCAGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCCGCTCATATATCAGAAATCAAAAAGCAGGTGGAGGTAGCAGAAGCCAAACTTGTCGTCACGAACAGCGCAAACTTTGAAAAGGTGAAGGAATTAAAGCTACCAGTGATCTTATTAGGGGAGGAACTAATTGAAGGTTCCATTAATTGGCACAAACTGCTTGAAGCTGCAGACCGTGCAGGCAACGATTGTGTTAAAGAAGATATTAAGCAGACTGATTTATGTGCCCTCCCTTTCTCATCAGGCACCACAGGGGTTTCCAAAGGCGTTATGCTAACTCACCGAAATCTAATAGCTAATTTGTGTTCGACTCTCTCCGGTGTCCCAAGAGAAATAGAGGGCAAGGTCACGACGTTAGGCCTCATTCCGTTCTTCCATATTTACGGGATCACTGGAATATGTTGTGCTGCACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACCTTCATTAATGCCCTCATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCCATCATCCTGGCTTTGGTTAAGAATCCTATTGTCGAAGAATTTGATCTTAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAAGTTCAGACTGCCTTCGAAAGAAAGTTCCCAGGCGTGGACATTCAAGAGTCAATAGATTGTGACAGATCTCTACTCTTGTTCATTCCTTCAAACTTTTTAGTTATGACATGTAGTACAACTTCCTGTTTTCGGCATATCAGAAGAAAACCGAGCTCCACCGCATATGGACTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGTATGGGCAAAGATAATCTCACTGCAAAGAAAAACACAGTTGGCCGCATTCTTCCTAATCTAGAAGTCAAATTCATCGATCCAGACAGCGGAAGGTCTCTGCCAAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGTACGGAACGTTACTACAAAAATGAAGAGGAGACTTCAAGAACGATCGATAACAAAGGCTGGATGCACACTGGGGACATTGGATACATTGATGACGATGGCGACGTGTTTATTGTGGATCGTATTAAGGAATTGATCAAGTACAAAGGCTTTCAAGTTGCTCCAGCTGAGTTAGAGGCAATCCTCCTTGCTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGCTGCCAGACGAAGAAGCCGGTGAGATCCCAGCAGCAAGTGTCGTGATGGCTCCGAATTCAAAAGAAACTGAAGATGAAATAATCAAACATGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTGGACACTATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGCTCGAAAAGATTCGAGCTGAATCCGCCTCTTAG

Coding sequence (CDS)

ATGGAGGGAAGTCGACCCTTATCGACACTTGCAGAAAACAACAACTCCGCCATGGCTACTTGCATTCGAGATTTGGTAGAAGAAGAGGAACACATTTTCCGCAGCCAACTTCCTGAGGTCCAAGTGCCTGATAATATCACACTGCCGGAGTTTGTACTTCAGAATGCTGAATCATATGCTGAAAATGTGGCATTTGTGGAAGCCGTGAGTGGAAAGGCTTACACTTACCGCGAAGTTGTGAGAGACATAAATAGATTTTCCAAGGCCTTGTGCTCTCTGAGGTTAAAGAAGGGGCAGGTAGTTATTGTTGTTCTACCCAATGTTGCAGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCCGCTCATATATCAGAAATCAAAAAGCAGGTGGAGGTAGCAGAAGCCAAACTTGTCGTCACGAACAGCGCAAACTTTGAAAAGGTGAAGGAATTAAAGCTACCAGTGATCTTATTAGGGGAGGAACTAATTGAAGGTTCCATTAATTGGCACAAACTGCTTGAAGCTGCAGACCGTGCAGGCAACGATTGTGTTAAAGAAGATATTAAGCAGACTGATTTATGTGCCCTCCCTTTCTCATCAGGCACCACAGGGGTTTCCAAAGGCGTTATGCTAACTCACCGAAATCTAATAGCTAATTTGTGTTCGACTCTCTCCGGTGTCCCAAGAGAAATAGAGGGCAAGGTCACGACGTTAGGCCTCATTCCGTTCTTCCATATTTACGGGATCACTGGAATATGTTGTGCTGCACTTAGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTCAGGACCTTCATTAATGCCCTCATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCCATCATCCTGGCTTTGGTTAAGAATCCTATTGTCGAAGAATTTGATCTTAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAAGTTCAGACTGCCTTCGAAAGAAAGTTCCCAGGCGTGGACATTCAAGAGTCAATAGATTGTGACAGATCTCTACTCTTGTTCATTCCTTCAAACTTTTTAGTTATGACATGTAGTACAACTTCCTGTTTTCGGCATATCAGAAGAAAACCGAGCTCCACCGCATATGGACTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGTATGGGCAAAGATAATCTCACTGCAAAGAAAAACACAGTTGGCCGCATTCTTCCTAATCTAGAAGTCAAATTCATCGATCCAGACAGCGGAAGGTCTCTGCCAAAGAACACTCCGGGTGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGTACGGAACGTTACTACAAAAATGAAGAGGAGACTTCAAGAACGATCGATAACAAAGGCTGGATGCACACTGGGGACATTGGATACATTGATGACGATGGCGACGTGTTTATTGTGGATCGTATTAAGGAATTGATCAAGTACAAAGGCTTTCAAGTTGCTCCAGCTGAGTTAGAGGCAATCCTCCTTGCTCATCCCTCCATTGAAGATGCAGCTGTGGTGCCGCTGCCAGACGAAGAAGCCGGTGAGATCCCAGCAGCAAGTGTCGTGATGGCTCCGAATTCAAAAGAAACTGAAGATGAAATAATCAAACATGTTGCCTCAAATGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTGGACACTATCCCGAAGTCGCCTTCTGGGAAAGTTATGAGAAGGTTGATCAAAGAGAAGATGCTCGAAAAGATTCGAGCTGAATCCGCCTCTTAG

Protein sequence

MEGSRPLSTLAENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLLEAADRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEGKVTTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIRAESAS
Homology
BLAST of CmUC11G217210 vs. NCBI nr
Match: XP_038893074.1 (4-coumarate--CoA ligase-like 1 [Benincasa hispida])

HSP 1 Score: 1010.4 bits (2611), Expect = 6.7e-291
Identity = 526/590 (89.15%), Postives = 542/590 (91.86%), Query Frame = 0

Query: 18  MATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 77
           MATC RDLVE+EEHIFRSQLPEVQVPD+ITLPEFVLQNAESYAENVAFVEAVSGKAYTYR
Sbjct: 1   MATCNRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYR 60

Query: 78  EVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 137
           EVVRD NRFSKAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI
Sbjct: 61  EVVRDTNRFSKALWSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 120

Query: 138 KKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLLEAADRAGNDCVKE 197
           KKQVEVAEAKLV+TNSANFEKVKELKLPVILLGEELIEGS+NWHKLLEAADRAGN+CVKE
Sbjct: 121 KKQVEVAEAKLVITNSANFEKVKELKLPVILLGEELIEGSMNWHKLLEAADRAGNNCVKE 180

Query: 198 DIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEGKVTTLGLIPFFHI 257
           DIKQ DLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVP+E+EGKVTTLGLIPFFHI
Sbjct: 181 DIKQNDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPQEMEGKVTTLGLIPFFHI 240

Query: 258 YGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 317
           YGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD
Sbjct: 241 YGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFD 300

Query: 318 LSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTS 377
           LSGLKLQAIMTAAAPLAPE+Q+AFERKFPGVD+QE                         
Sbjct: 301 LSGLKLQAIMTAAAPLAPELQSAFERKFPGVDVQE------------------------- 360

Query: 378 CFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRS 437
                       AYGLTEHSCITLNYGS+GK+NL AKKNTVGRILPNLEVKFIDPDSGRS
Sbjct: 361 ------------AYGLTEHSCITLNYGSIGKENLAAKKNTVGRILPNLEVKFIDPDSGRS 420

Query: 438 LPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRI 497
           LPKNTPGEICVRSQCVMQG   YYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRI
Sbjct: 421 LPKNTPGEICVRSQCVMQG---YYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRI 480

Query: 498 KELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEI 557
           KELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETE+EI
Sbjct: 481 KELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEEEI 540

Query: 558 IKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIRAESAS 608
           IKHVASNVA+YKKVRLVHFVD IPKSPSGKVMRRL+KEKM+EKIRAESAS
Sbjct: 541 IKHVASNVANYKKVRLVHFVDAIPKSPSGKVMRRLVKEKMIEKIRAESAS 550

BLAST of CmUC11G217210 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1004.6 bits (2596), Expect = 3.7e-289
Identity = 523/605 (86.45%), Postives = 552/605 (91.24%), Query Frame = 0

Query: 3   GSRPLSTLAENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAEN 62
           G RP S  A NNNSAMATCIRDLVE+EEHIFRSQLPEVQVPD+ITLPEFVLQNAESYA+N
Sbjct: 43  GRRP-SMSAANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADN 102

Query: 63  VAFVEAVSGKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAG 122
           VAFVEAVSGKAYTYREVVRD +RF+KAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAG
Sbjct: 103 VAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAG 162

Query: 123 GVFSGVNPAAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHK 182
           GVFSGVNPAAHISEIKKQVEVA+AKLVVTNS++FEKVKELKLPVI++GEELIEGS+NWHK
Sbjct: 163 GVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHK 222

Query: 183 LLEAADRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI 242
           LLEAADRAGN+ VKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVP+E+
Sbjct: 223 LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM 282

Query: 243 EGKVTTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP 302
            GKVTTLGLIPFFHIYGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP
Sbjct: 283 VGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP 342

Query: 303 IILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLL 362
           IILALVKNPIV+EFDLSGLKLQAIMTAAAPLAPE+QT+FE+KFPGVD+QE          
Sbjct: 343 IILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQE---------- 402

Query: 363 FIPSNFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRIL 422
                                      AYGLTEHSCITLNYGS+GKDNL AKKNTVGRIL
Sbjct: 403 ---------------------------AYGLTEHSCITLNYGSIGKDNLAAKKNTVGRIL 462

Query: 423 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDI 482
           PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG   YY NEEETSRTID+KGWMHTGDI
Sbjct: 463 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG---YYNNEEETSRTIDDKGWMHTGDI 522

Query: 483 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAA 542
           GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPD+EAGEIPAA
Sbjct: 523 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAA 582

Query: 543 SVVMAPNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIR 602
           S+VMAPN+KETEDEIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM+EKIR
Sbjct: 583 SIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIR 606

Query: 603 AESAS 608
           AE+AS
Sbjct: 643 AEAAS 606

BLAST of CmUC11G217210 vs. NCBI nr
Match: KAG7012722.1 (4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 999.2 bits (2582), Expect = 1.5e-287
Identity = 520/605 (85.95%), Postives = 550/605 (90.91%), Query Frame = 0

Query: 3   GSRPLSTLAENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAEN 62
           G RP S  A NNNSAMATCIRD VE+EEHIFRSQLPEVQVPD+ITLPEFVLQNAESYA+N
Sbjct: 43  GRRP-SMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADN 102

Query: 63  VAFVEAVSGKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAG 122
           VAFVEAVSGKAYTYREVVRD +RF+KAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAG
Sbjct: 103 VAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAG 162

Query: 123 GVFSGVNPAAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHK 182
           GVFSGVNPAAHISEIKKQVEVA+AKLVVTNS++FEKVKELKLPVI++GEELIEGS+NWH+
Sbjct: 163 GVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQ 222

Query: 183 LLEAADRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI 242
           LLEAADRAGN+ VKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVP+E+
Sbjct: 223 LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM 282

Query: 243 EGKVTTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP 302
            GKVTTLGLIPFFHIYGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP
Sbjct: 283 VGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP 342

Query: 303 IILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLL 362
           IILALVKNPIV+EFDLSGLKLQAIMTAAAPLAPE+QT+FE+KFPGVD+QE          
Sbjct: 343 IILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQE---------- 402

Query: 363 FIPSNFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRIL 422
                                      AYGLTEHSCITLNYGS+GKDNL AKKNTVGRIL
Sbjct: 403 ---------------------------AYGLTEHSCITLNYGSIGKDNLAAKKNTVGRIL 462

Query: 423 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDI 482
           PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG   YY NEEETSRTID+KGWMHTGDI
Sbjct: 463 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG---YYNNEEETSRTIDDKGWMHTGDI 522

Query: 483 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAA 542
           GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPD+EAGEIPAA
Sbjct: 523 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAA 582

Query: 543 SVVMAPNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIR 602
           S+VM PN+KETEDEIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM+EKIR
Sbjct: 583 SIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIR 606

Query: 603 AESAS 608
           AE+AS
Sbjct: 643 AETAS 606

BLAST of CmUC11G217210 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 996.1 bits (2574), Expect = 1.3e-286
Identity = 519/605 (85.79%), Postives = 549/605 (90.74%), Query Frame = 0

Query: 3   GSRPLSTLAENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAEN 62
           G R L + A  NNSAMATCIRD VE+EEHIFRSQLPEVQVPD+ITLPEFVLQNAESYA+N
Sbjct: 43  GRRSLMS-AATNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADN 102

Query: 63  VAFVEAVSGKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAG 122
           VAFVEAVSG AYTYREVVRD +RF+KAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAG
Sbjct: 103 VAFVEAVSGNAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAG 162

Query: 123 GVFSGVNPAAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHK 182
           GVFSGVNPAAHISEIKKQVEVA+AKLVVTNS++FEKVKELKLPVI++GEELIEGS+NWHK
Sbjct: 163 GVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHK 222

Query: 183 LLEAADRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI 242
           LLEAADRAGN+ VKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVP+E+
Sbjct: 223 LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM 282

Query: 243 EGKVTTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP 302
            GKVTTLGLIPFFHIYGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP
Sbjct: 283 VGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP 342

Query: 303 IILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLL 362
           IILALVKNPIV+EFDLSGLKLQAIMTAAAPLAPE+QT+FE+KFPGVD+QE          
Sbjct: 343 IILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQE---------- 402

Query: 363 FIPSNFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRIL 422
                                      AYGLTEHSCITLNYGS+GKDNL AKKNTVGRIL
Sbjct: 403 ---------------------------AYGLTEHSCITLNYGSIGKDNLAAKKNTVGRIL 462

Query: 423 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDI 482
           PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG   YY NEEETSRTID+KGWMHTGDI
Sbjct: 463 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG---YYNNEEETSRTIDDKGWMHTGDI 522

Query: 483 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAA 542
           GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPD+EAGEIPAA
Sbjct: 523 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAA 582

Query: 543 SVVMAPNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIR 602
           S+VMAPN+KETEDEIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM+EKIR
Sbjct: 583 SIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIR 606

Query: 603 AESAS 608
           AE+AS
Sbjct: 643 AETAS 606

BLAST of CmUC11G217210 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 993.0 bits (2566), Expect = 1.1e-285
Identity = 514/603 (85.24%), Postives = 545/603 (90.38%), Query Frame = 0

Query: 5   RPLSTLAENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVA 64
           R  S  A NNNS MATCIRD  E+EEHIFRSQLPEVQVPD+ITLPEFVLQNAE YA+NVA
Sbjct: 44  RRSSMSAANNNSTMATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVA 103

Query: 65  FVEAVSGKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGV 124
           FVEAVSGKAYTYREVVRD +RF+KAL SLRLKKGQ+VIVVLPNVAEYAIVALGIMAAGGV
Sbjct: 104 FVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGV 163

Query: 125 FSGVNPAAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLL 184
           FSGVNPAAHISEIKKQVEVA+AKLVVTNS++FEKVKELKLPVI++GEELIEGS+NWHKLL
Sbjct: 164 FSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLL 223

Query: 185 EAADRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEG 244
           EAADRAGN+ VKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSG+P+E+ G
Sbjct: 224 EAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVG 283

Query: 245 KVTTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII 304
           KVTTLGLIPFFHIYGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII
Sbjct: 284 KVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII 343

Query: 305 LALVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFI 364
           LALVKNPIV+EFDLS LKLQAIMTAAAPLAPE+QT+FE+KFPGVD+QE            
Sbjct: 344 LALVKNPIVKEFDLSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQE------------ 403

Query: 365 PSNFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPN 424
                                    AYGLTEHSCITLNYG++GKDNL AKKNTVGRILPN
Sbjct: 404 -------------------------AYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPN 463

Query: 425 LEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGY 484
           LEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG   YY NEEETSRTIDNKGWMHTGDIGY
Sbjct: 464 LEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG---YYNNEEETSRTIDNKGWMHTGDIGY 523

Query: 485 IDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASV 544
           IDDDGDVFIVDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPD+EAGEIPAAS+
Sbjct: 524 IDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASI 583

Query: 545 VMAPNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIRAE 604
           VMAPN+KETEDEIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM+EKIRAE
Sbjct: 584 VMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAE 606

Query: 605 SAS 608
           +AS
Sbjct: 644 TAS 606

BLAST of CmUC11G217210 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 757.3 bits (1954), Expect = 1.3e-217
Identity = 389/572 (68.01%), Postives = 452/572 (79.02%), Query Frame = 0

Query: 27  EEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDINRF 86
           E+ E+IFRS  P V +PD +TLPEFVLQ  E Y ENVAFVEAV+GKA TY +VVRD  R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 87  SKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEA 146
           +KAL SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 147 KLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLLEAADRAGNDCVKEDIKQTDLCA 206
           + ++T++ N+EKVK L LPVI+LGEE IEG++NW  LLEA D+ G D   E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCG-DTDNEEILQTDLCA 186

Query: 207 LPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEGKVTTLGLIPFFHIYGITGICCA 266
           LPFSSGTTG+ KGVMLTHRNLIANLCSTL GV  E+ G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 267 ALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 326
            ++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 327 MTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRHIRRKP 386
           MTAAAPLAPE+ TAFE KFP V +QE                                  
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQE---------------------------------- 366

Query: 387 SSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEI 446
              AYGLTEHSCITL +G   K    AK+N+VG ILPNLEVKFIDPD+GRSLPKNT GE+
Sbjct: 367 ---AYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILPNLEVKFIDPDTGRSLPKNTSGEL 426

Query: 447 CVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGF 506
           CVRSQCVMQG   Y+ N+EET +TID +GW+HTGDIGYIDDDGD+FIVDRIKELIKYKGF
Sbjct: 427 CVRSQCVMQG---YFMNKEETDKTIDEQGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGF 486

Query: 507 QVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHVASNVA 566
           QVAPAELEAILL HPS+ED AVVPLPDEEAGEIPAA VV+ P + E E++I+  VA+NVA
Sbjct: 487 QVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVINPKATEKEEDILNFVAANVA 537

Query: 567 HYKKVRLVHFVDTIPKSPSGKVMRRLIKEKML 599
           HYKKVR VHFVD+IPKS SGK+MRRL+++K+L
Sbjct: 547 HYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of CmUC11G217210 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 673.3 bits (1736), Expect = 2.5e-192
Identity = 345/579 (59.59%), Postives = 438/579 (75.65%), Query Frame = 0

Query: 27  EEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVS-GKAYTYREVVRDINR 86
           EE+EH+FRS+ P V VPD +T+PEFVL  AE+YA+ VA VEA + G++YTY EV RD  R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 87  FSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAE 146
           F++AL S+ ++KG VV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE +E
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 147 AKLVVTNSANFEKVKELKLPVILLGE-ELIEGSINWHKLLEAADRAGNDCVKED-IKQTD 206
           AKLVV N   F+KVK+  +PVI +G+ E + G+I+W  LL AADR G   V  D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 207 LCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEGKVTTLGLIPFFHIYGITGI 266
           LCALP+SSGTTGVSKGVML+HRNL++NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 267 CCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKL 326
           CCA LR+KG VVVM RFDLRTF+ AL+   V FAP+VPP++LA+VK+P+ +EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 327 QAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRHIR 386
           +++MTAAAPLAP++  AF+RKFPGV ++E                               
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEE------------------------------- 373

Query: 387 RKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKNTP 446
                 AYGLTEHSCITL + +       AKK++VG ILPNLEVKF+DPD+GRSLP NTP
Sbjct: 374 ------AYGLTEHSCITLTHAAGDGHGHVAKKSSVGFILPNLEVKFVDPDTGRSLPANTP 433

Query: 447 GEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKY 506
           GE+CVRSQ VMQG   YYK +EET RT+D KGW+HTGD+GYID DGDVFIVDRIKELIKY
Sbjct: 434 GELCVRSQSVMQG---YYKRKEETERTVDGKGWLHTGDVGYIDGDGDVFIVDRIKELIKY 493

Query: 507 KGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHVAS 566
           KGFQVAPAELEA+LL+HPS+EDAAV  +PDEEAGE+P A VV    ++E E+EI+ +VA 
Sbjct: 494 KGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVACVVRRHGAEEGEEEIVAYVAE 552

Query: 567 NVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIR 603
            VA YK+VR++H VD IPKS SGK++RR ++++ +++++
Sbjct: 554 RVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

BLAST of CmUC11G217210 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 382.9 bits (982), Expect = 6.9e-105
Identity = 201/571 (35.20%), Postives = 328/571 (57.44%), Query Frame = 0

Query: 32  IFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDINRFSKALC 91
           IFRS+LP++ +P ++ L  +  +N   +      ++  + + YTY EV     + +  L 
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 92  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 151
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AK+V+T
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVIT 133

Query: 152 NSANFEKVKELKLP---VILLGEELIEGSINWHKLLEAADRAGNDCVKEDIKQTDLCALP 211
            +    KVK+  +     ++  + + EG +++ +L+++ +    D     I+  D+ ALP
Sbjct: 134 QACFAGKVKDYAIENDLKVICVDSVPEGCVHFSELIQSDEHEIPDV---KIQPDDVVALP 193

Query: 212 FSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI--EGKVTTLGLIPFFHIYGITGICCA 271
           +SSGTTG+ KGVMLTH+ L+ ++   + G    +        + ++P FHIY +  +   
Sbjct: 194 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLLC 253

Query: 272 ALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 331
           ALR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V+ +DLS ++   +
Sbjct: 254 ALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVR--TV 313

Query: 332 MTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRHIRRKP 391
           M+ AAPL  E++ A   KFP   + +                                  
Sbjct: 314 MSGAAPLGKELEDAVRAKFPNAKLGQ---------------------------------- 373

Query: 392 SSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEI 451
               YG+TE   +     +  K+    K    G ++ N E+K +DPD+G SLP+N PGEI
Sbjct: 374 ---GYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEI 433

Query: 452 CVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGF 511
           C+R   +M+G   Y  + E T+RTI+ +GW+HTGDIG+IDDD ++FIVDR+KELIKYKGF
Sbjct: 434 CIRGDQIMKG---YLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGF 493

Query: 512 QVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHVASNVA 571
           QVAPAELEA+L+ HP I DAAVVP+ DE+AGE+P A VV +  S  TEDE+   ++  V 
Sbjct: 494 QVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVI 539

Query: 572 HYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 598
            YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 554 FYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of CmUC11G217210 vs. ExPASy Swiss-Prot
Match: P31685 (4-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 2.0e-104
Identity = 201/571 (35.20%), Postives = 326/571 (57.09%), Query Frame = 0

Query: 32  IFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDINRFSKALC 91
           IFRS+LP++ +P ++ L  +  +N   +      ++  + + YTY EV     + +  L 
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 92  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 151
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AK+V+T
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVIT 133

Query: 152 NSANFEKVKELKLP---VILLGEELIEGSINWHKLLEAADRAGNDCVKEDIKQTDLCALP 211
            +    KVK+  +     ++  +   EG +++ +L+++ +    D     I+  D+ ALP
Sbjct: 134 QACFAGKVKDYAIENDLKVICVDSAPEGCVHFSELIQSDEHEIPDV---KIQPDDVVALP 193

Query: 212 FSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI--EGKVTTLGLIPFFHIYGITGICCA 271
           +SSGTTG+ KGVMLTH+ L+ ++   + G    +        + ++P FHIY +  +   
Sbjct: 194 YSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLLC 253

Query: 272 ALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 331
           ALR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V  +DLS ++   +
Sbjct: 254 ALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVHNYDLSSVR--TV 313

Query: 332 MTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRHIRRKP 391
           M+ AAPL  E++ A   KFP   + +                                  
Sbjct: 314 MSGAAPLGKELEDAVRAKFPNAKLGQ---------------------------------- 373

Query: 392 SSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEI 451
               YG+TE   +     +  K+    K    G ++ N E+K +DPD+G SLP+N PGEI
Sbjct: 374 ---GYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEI 433

Query: 452 CVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGF 511
           C+R   +M+G   Y  + E T+RTI+ +GW+HTGDIG+IDDD ++FIVDR+KELIKYKGF
Sbjct: 434 CIRGDQIMKG---YLNDPEATARTIEKEGWLHTGDIGFIDDDDELFIVDRLKELIKYKGF 493

Query: 512 QVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHVASNVA 571
           QVAPAELEA+L+ HP I DAAVVP+ DE+AGE+P A VV +  S  TEDE+   ++  V 
Sbjct: 494 QVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVVRSNGSTITEDEVKDFISKQVI 539

Query: 572 HYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 598
            YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 554 FYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

BLAST of CmUC11G217210 vs. ExPASy Swiss-Prot
Match: I3PB37 (4-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 2.6e-104
Identity = 206/572 (36.01%), Postives = 326/572 (56.99%), Query Frame = 0

Query: 32  IFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDINRFSKALC 91
           IFRS+LP++ +P ++ L  +  +N   ++     +   +   YTY +V     + +  L 
Sbjct: 13  IFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANNHIYTYADVELTSRKVAAGLN 72

Query: 92  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 151
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AKL++T
Sbjct: 73  KLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVKQAKASNAKLIIT 132

Query: 152 NSANFEKVKEL----KLPVILLGEELIEGSINWHKLLEAADRAGNDCVKEDIKQTDLCAL 211
            +    KVK+      L VI + +   EG I++ +L +A +   +D     I+  D+ AL
Sbjct: 133 QACFVNKVKDYAFDNNLNVICI-DSAPEGCIHFSELTQADE---HDIPDVKIQSDDVVAL 192

Query: 212 PFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI--EGKVTTLGLIPFFHIYGITGICC 271
           P+SSGTTG+ KGVMLTH+ L+ ++   + G    +    +   + ++P FHIY +  +  
Sbjct: 193 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLFHIYSLNSVLL 252

Query: 272 AALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQA 331
             LR    +++M +FD+  F   +   +VT  P VPPI+LA+ K+P+V+ +DLS ++   
Sbjct: 253 CGLRVGAAILIMQKFDIVQFCELIEKYKVTIGPFVPPIVLAIAKSPVVDNYDLSSVR--T 312

Query: 332 IMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRHIRRK 391
           +M+ AAPL  E++ A   KFP   + +                                 
Sbjct: 313 VMSGAAPLGKELEDAVRIKFPNAKLGQ--------------------------------- 372

Query: 392 PSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGE 451
                YG+TE   +     +  K+    K    G ++ N E+K +DPD+G SLP+N PGE
Sbjct: 373 ----GYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGE 432

Query: 452 ICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKG 511
           IC+R   +M+G   Y  +   T+RTID +GW+HTGDIGYID+D ++FIVDR+KELIKYKG
Sbjct: 433 ICIRGDQIMKG---YLNDPAATTRTIDKEGWLHTGDIGYIDNDDELFIVDRLKELIKYKG 492

Query: 512 FQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHVASNV 571
           FQVAPAELEA+LL HP+I DAAVVP+ DE+AGE+P A VV +  S  TEDE+   V+  V
Sbjct: 493 FQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFVVRSNGSDITEDEVKDFVSKQV 538

Query: 572 AHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 598
             YK+++ V FV+T+PKSPSGK++R+ ++ ++
Sbjct: 553 IFYKRIKRVFFVETVPKSPSGKILRKDLRARL 538

BLAST of CmUC11G217210 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1004.6 bits (2596), Expect = 1.8e-289
Identity = 523/605 (86.45%), Postives = 552/605 (91.24%), Query Frame = 0

Query: 3   GSRPLSTLAENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAEN 62
           G RP S  A NNNSAMATCIRDLVE+EEHIFRSQLPEVQVPD+ITLPEFVLQNAESYA+N
Sbjct: 43  GRRP-SMSAANNNSAMATCIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADN 102

Query: 63  VAFVEAVSGKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAG 122
           VAFVEAVSGKAYTYREVVRD +RF+KAL SLRLKKGQVVIVVLPNVAEYAIVALGIMAAG
Sbjct: 103 VAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAG 162

Query: 123 GVFSGVNPAAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHK 182
           GVFSGVNPAAHISEIKKQVEVA+AKLVVTNS++FEKVKELKLPVI++GEELIEGS+NWHK
Sbjct: 163 GVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHK 222

Query: 183 LLEAADRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI 242
           LLEAADRAGN+ VKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVP+E+
Sbjct: 223 LLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM 282

Query: 243 EGKVTTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP 302
            GKVTTLGLIPFFHIYGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP
Sbjct: 283 VGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPP 342

Query: 303 IILALVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLL 362
           IILALVKNPIV+EFDLSGLKLQAIMTAAAPLAPE+QT+FE+KFPGVD+QE          
Sbjct: 343 IILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQE---------- 402

Query: 363 FIPSNFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRIL 422
                                      AYGLTEHSCITLNYGS+GKDNL AKKNTVGRIL
Sbjct: 403 ---------------------------AYGLTEHSCITLNYGSIGKDNLAAKKNTVGRIL 462

Query: 423 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDI 482
           PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG   YY NEEETSRTID+KGWMHTGDI
Sbjct: 463 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG---YYNNEEETSRTIDDKGWMHTGDI 522

Query: 483 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAA 542
           GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPD+EAGEIPAA
Sbjct: 523 GYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAA 582

Query: 543 SVVMAPNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIR 602
           S+VMAPN+KETEDEIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM+EKIR
Sbjct: 583 SIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIR 606

Query: 603 AESAS 608
           AE+AS
Sbjct: 643 AEAAS 606

BLAST of CmUC11G217210 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 993.0 bits (2566), Expect = 5.3e-286
Identity = 514/603 (85.24%), Postives = 545/603 (90.38%), Query Frame = 0

Query: 5   RPLSTLAENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVA 64
           R  S  A NNNS MATCIRD  E+EEHIFRSQLPEVQVPD+ITLPEFVLQNAE YA+NVA
Sbjct: 44  RRSSMSAANNNSTMATCIRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVA 103

Query: 65  FVEAVSGKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGV 124
           FVEAVSGKAYTYREVVRD +RF+KAL SLRLKKGQ+VIVVLPNVAEYAIVALGIMAAGGV
Sbjct: 104 FVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGV 163

Query: 125 FSGVNPAAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLL 184
           FSGVNPAAHISEIKKQVEVA+AKLVVTNS++FEKVKELKLPVI++GEELIEGS+NWHKLL
Sbjct: 164 FSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLL 223

Query: 185 EAADRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEG 244
           EAADRAGN+ VKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSG+P+E+ G
Sbjct: 224 EAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVG 283

Query: 245 KVTTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII 304
           KVTTLGLIPFFHIYGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII
Sbjct: 284 KVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII 343

Query: 305 LALVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFI 364
           LALVKNPIV+EFDLS LKLQAIMTAAAPLAPE+QT+FE+KFPGVD+QE            
Sbjct: 344 LALVKNPIVKEFDLSSLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQE------------ 403

Query: 365 PSNFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPN 424
                                    AYGLTEHSCITLNYG++GKDNL AKKNTVGRILPN
Sbjct: 404 -------------------------AYGLTEHSCITLNYGNIGKDNLAAKKNTVGRILPN 463

Query: 425 LEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGY 484
           LEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG   YY NEEETSRTIDNKGWMHTGDIGY
Sbjct: 464 LEVKFIDPDSGRSLPKNTPGEICVRSQCVMQG---YYNNEEETSRTIDNKGWMHTGDIGY 523

Query: 485 IDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASV 544
           IDDDGDVFIVDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPD+EAGEIPAAS+
Sbjct: 524 IDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASI 583

Query: 545 VMAPNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIRAE 604
           VMAPN+KETEDEIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKM+EKIRAE
Sbjct: 584 VMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAE 606

Query: 605 SAS 608
           +AS
Sbjct: 644 TAS 606

BLAST of CmUC11G217210 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 982.6 bits (2539), Expect = 7.2e-283
Identity = 511/600 (85.17%), Postives = 543/600 (90.50%), Query Frame = 0

Query: 8   STLAENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVE 67
           S++   N SAM T IRDLVE+EEHIFRSQLPEVQVPD+ITLPEFVLQNAESYAENVAFVE
Sbjct: 50  SSMPVANISAMGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVE 109

Query: 68  AVSGKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSG 127
           AVSGKAYTYREVVRD  RF+KAL SLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSG
Sbjct: 110 AVSGKAYTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSG 169

Query: 128 VNPAAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLLEAA 187
           VNPAAHISEIKKQVE A+AKLVVTNS NFEKVKELKLPVI+LGEELIEGS+NWHKLLEAA
Sbjct: 170 VNPAAHISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAA 229

Query: 188 DRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEGKVT 247
           DRAGN+ VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVPRE EGKVT
Sbjct: 230 DRAGNNHVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVT 289

Query: 248 TLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILAL 307
           TLGLIPFFHIYGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILAL
Sbjct: 290 TLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILAL 349

Query: 308 VKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSN 367
           VKNPIVEEFDLS LKLQAIMTAAAPLAPE+QTAFE+KFPGVD+QE               
Sbjct: 350 VKNPIVEEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQE--------------- 409

Query: 368 FLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEV 427
                                 AYGLTEHSCITLNYGS+GK+NLTAKKNTVGRILPNLEV
Sbjct: 410 ----------------------AYGLTEHSCITLNYGSIGKENLTAKKNTVGRILPNLEV 469

Query: 428 KFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDD 487
           KFIDP+SGRSLPKNTPGEICVRSQCVMQG   YY NEEETSRTIDN+GW+HTGDIGYIDD
Sbjct: 470 KFIDPESGRSLPKNTPGEICVRSQCVMQG---YYNNEEETSRTIDNRGWLHTGDIGYIDD 529

Query: 488 DGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMA 547
           +GDVFIVDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPDEEAGEIPAASVV+A
Sbjct: 530 NGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVIA 589

Query: 548 PNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIRAESAS 607
           PNSKETE+EII++VASNVA+YKKVR+VHFVD+IPKSPSGKVMRRLIKEKM++KIRA++AS
Sbjct: 590 PNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKMIDKIRAKTAS 609

BLAST of CmUC11G217210 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 982.2 bits (2538), Expect = 9.4e-283
Identity = 510/594 (85.86%), Postives = 540/594 (90.91%), Query Frame = 0

Query: 14  NNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKA 73
           N SAM T IRDLVE+EEHIFRSQLPEVQVPD+ITLPEFVLQNAESYAENVAFVEAVSGKA
Sbjct: 5   NISAMGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKA 64

Query: 74  YTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH 133
           YTYREVVRD  RF+KAL SLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH
Sbjct: 65  YTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH 124

Query: 134 ISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLLEAADRAGND 193
           ISEIKKQVE A+AKLVVTNS NFEKVKELKLPVI+LGEELIEGS+NWHKLLEAADRAGN+
Sbjct: 125 ISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNN 184

Query: 194 CVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEGKVTTLGLIP 253
            VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNL+ANLCSTLSGVPRE EGKVTTLGLIP
Sbjct: 185 HVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIP 244

Query: 254 FFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV 313
           FFHIYGITGICCA LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV
Sbjct: 245 FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV 304

Query: 314 EEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTC 373
           EEFDLS LKLQAIMTAAAPLAPE+QTAFE+KFPGVD+QE                     
Sbjct: 305 EEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQE--------------------- 364

Query: 374 STTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPD 433
                           AYGLTEHSCITLNYGS+GK+NLTAKKNTVGRILPNLEVKFIDP+
Sbjct: 365 ----------------AYGLTEHSCITLNYGSIGKENLTAKKNTVGRILPNLEVKFIDPE 424

Query: 434 SGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFI 493
           SGRSLPKNTPGEICVRSQCVMQG   YY NEEETSRTIDN+GW+HTGDIGYIDD+GDVFI
Sbjct: 425 SGRSLPKNTPGEICVRSQCVMQG---YYNNEEETSRTIDNRGWLHTGDIGYIDDNGDVFI 484

Query: 494 VDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKET 553
           VDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPDEEAGEIPAASVV+APNSKET
Sbjct: 485 VDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVIAPNSKET 544

Query: 554 EDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIRAESAS 608
           E+EII++VASNVA+YKKVR+VHFVD+IPKSPSGKVMRRLIKEKM++KIRA++AS
Sbjct: 545 EEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKMIDKIRAKTAS 558

BLAST of CmUC11G217210 vs. ExPASy TrEMBL
Match: A0A5D3CV41 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold35G002330 PE=4 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.8e-279
Identity = 503/597 (84.25%), Postives = 537/597 (89.95%), Query Frame = 0

Query: 11  AENNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVS 70
           AEN  SAM T  +D V++E+HIFRSQLPEVQVP +ITLPEFVLQNAESYAENVAFVEA+S
Sbjct: 20  AENYYSAMTTRNQDSVQDEKHIFRSQLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMS 79

Query: 71  GKAYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNP 130
           GKAYTYREV+RD NRFSKAL SLRLKKG VV+VVLPNVAEYAIVALGIM AGGVFSGVNP
Sbjct: 80  GKAYTYREVLRDTNRFSKALHSLRLKKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNP 139

Query: 131 AAHISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLLEAADRA 190
           AAHISEIKKQVEVAEAKLVVTN+ANFEKV+ELKLPVILL EEL+EG++NWHKLLEAADRA
Sbjct: 140 AAHISEIKKQVEVAEAKLVVTNAANFEKVRELKLPVILLEEELMEGAMNWHKLLEAADRA 199

Query: 191 GNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEGKVTTLG 250
           GN+ VKE+IKQTDLCALPFSSGTTGVSKGVMLTHRNL+AN+CSTLSGVP+E+EGKVTTLG
Sbjct: 200 GNNFVKEEIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLG 259

Query: 251 LIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKN 310
           LIPFFHIYGITGICC+ LRNKGKVVVMGRFDLRTFINALIT E+TFAPIVPPIILALVKN
Sbjct: 260 LIPFFHIYGITGICCSTLRNKGKVVVMGRFDLRTFINALITHEITFAPIVPPIILALVKN 319

Query: 311 PIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLV 370
           PIVEEFDLS LKLQAIMTAAAPLAPE+QTAFERKFPGVD+QE                  
Sbjct: 320 PIVEEFDLSSLKLQAIMTAAAPLAPELQTAFERKFPGVDVQE------------------ 379

Query: 371 MTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFI 430
                              AYGLTEH CITLNYG++GK+NL+AKKNTVGRILPNLEVKFI
Sbjct: 380 -------------------AYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFI 439

Query: 431 DPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGD 490
           DPD+GRSLPKNTPGEICVRSQCVMQG   YYKNEEETSRTID+KGWMHTGDIGYIDDDGD
Sbjct: 440 DPDTGRSLPKNTPGEICVRSQCVMQG---YYKNEEETSRTIDSKGWMHTGDIGYIDDDGD 499

Query: 491 VFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS 550
           VFIVDRIKELIKYKGFQVAPAELEAILL HPSIEDAAVVPLPDEEAGEIPAASVVMAPNS
Sbjct: 500 VFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNS 559

Query: 551 KETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMLEKIRAESAS 608
            ETE+EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM+EKIRA+ ++
Sbjct: 560 NETEEEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 576

BLAST of CmUC11G217210 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 757.3 bits (1954), Expect = 9.5e-219
Identity = 389/572 (68.01%), Postives = 452/572 (79.02%), Query Frame = 0

Query: 27  EEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDINRF 86
           E+ E+IFRS  P V +PD +TLPEFVLQ  E Y ENVAFVEAV+GKA TY +VVRD  R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 87  SKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEA 146
           +KAL SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 147 KLVVTNSANFEKVKELKLPVILLGEELIEGSINWHKLLEAADRAGNDCVKEDIKQTDLCA 206
           + ++T++ N+EKVK L LPVI+LGEE IEG++NW  LLEA D+ G D   E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCG-DTDNEEILQTDLCA 186

Query: 207 LPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIEGKVTTLGLIPFFHIYGITGICCA 266
           LPFSSGTTG+ KGVMLTHRNLIANLCSTL GV  E+ G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 267 ALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAI 326
            ++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV+EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 327 MTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRHIRRKP 386
           MTAAAPLAPE+ TAFE KFP V +QE                                  
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQE---------------------------------- 366

Query: 387 SSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEI 446
              AYGLTEHSCITL +G   K    AK+N+VG ILPNLEVKFIDPD+GRSLPKNT GE+
Sbjct: 367 ---AYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILPNLEVKFIDPDTGRSLPKNTSGEL 426

Query: 447 CVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGF 506
           CVRSQCVMQG   Y+ N+EET +TID +GW+HTGDIGYIDDDGD+FIVDRIKELIKYKGF
Sbjct: 427 CVRSQCVMQG---YFMNKEETDKTIDEQGWLHTGDIGYIDDDGDIFIVDRIKELIKYKGF 486

Query: 507 QVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHVASNVA 566
           QVAPAELEAILL HPS+ED AVVPLPDEEAGEIPAA VV+ P + E E++I+  VA+NVA
Sbjct: 487 QVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVINPKATEKEEDILNFVAANVA 537

Query: 567 HYKKVRLVHFVDTIPKSPSGKVMRRLIKEKML 599
           HYKKVR VHFVD+IPKS SGK+MRRL+++K+L
Sbjct: 547 HYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

BLAST of CmUC11G217210 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 374.8 bits (961), Expect = 1.3e-103
Identity = 205/591 (34.69%), Postives = 332/591 (56.18%), Query Frame = 0

Query: 13  NNNSAMATCIRDLVEEEEHIFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGK 72
           N+ +    C  D+      IFRS+LP++ +P+++ L +++ +N   +A     +   +G+
Sbjct: 9   NDQNDQKQCSNDV------IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGE 68

Query: 73  AYTYREVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAA 132
            YTY +V     + +  L +L +K+  VV+++LPN  E  +  L     G + +  NP  
Sbjct: 69  VYTYADVHVTSRKLAAGLHNLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFF 128

Query: 133 HISEIKKQVEVAEAKLVVTNSANFEKVKELKLPVILL----GEELIEGSINWHKLLEAAD 192
             +EI KQ + + AKL+VT S   +K+K L+   +L+     + + E  + + +L + ++
Sbjct: 129 TPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQ-SE 188

Query: 193 RAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI--EGKV 252
               D + E I   D+ ALPFSSGTTG+ KGVMLTH+ L+ ++   + G    +      
Sbjct: 189 EPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDD 248

Query: 253 TTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILA 312
             L ++P FHIY +  I   +LR    +++M +F++   +  +   +VT A +VPPI+LA
Sbjct: 249 VILCVLPMFHIYALNSIMLCSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLA 308

Query: 313 LVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPS 372
           + K+P  E++DLS +++  + + AAPL  E++ A   KFP   + +              
Sbjct: 309 IAKSPETEKYDLSSVRM--VKSGAAPLGKELEDAISAKFPNAKLGQ-------------- 368

Query: 373 NFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLE 432
                                   YG+TE   +        K+    K    G ++ N E
Sbjct: 369 -----------------------GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAE 428

Query: 433 VKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYID 492
           +K +DPD+G SLP+N PGEIC+R   +M+G   Y  +   T+ TID  GW+HTGD+G+ID
Sbjct: 429 MKILDPDTGDSLPRNKPGEICIRGNQIMKG---YLNDPLATASTIDKDGWLHTGDVGFID 488

Query: 493 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVM 552
           DD ++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +E+AGE+P A VV 
Sbjct: 489 DDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVVR 548

Query: 553 APNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 598
           + +S  +EDEI + V+  V  YK++  V F D+IPK+PSGK++R+ ++ ++
Sbjct: 549 SKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

BLAST of CmUC11G217210 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 360.5 bits (924), Expect = 2.6e-99
Identity = 196/576 (34.03%), Postives = 319/576 (55.38%), Query Frame = 0

Query: 32  IFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDINRFSKALC 91
           IFRS+LP++ +P++++L +++ QN   +A     +   +G  YTY +V     + +    
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 92  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 151
            L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + +  KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 152 NSANFEKVKELK----LPVILLGEE----LIEGSINWHKLLEAADRAGNDCVKEDIKQTD 211
            +   +K+K L+    + ++ + +     + EG + + +L ++   A       +I   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 212 LCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREI--EGKVTTLGLIPFFHIYGIT 271
           + ALP+SSGTTG+ KGVMLTH+ L+ ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 272 GICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGL 331
            I    LR    +++M +F++   +  +   +VT AP+VPPI+LA+ K+   E++DLS +
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 332 KLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRH 391
           ++  + + AAPL  E++ A   KFP   + +                             
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQ----------------------------- 383

Query: 392 IRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKN 451
                    YG+TE   +        K+    K    G ++ N E+K +DPD+G SL +N
Sbjct: 384 --------GYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVVRNAEMKIVDPDTGDSLSRN 443

Query: 452 TPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELI 511
            PGEIC+R   +M+G   Y  N   T+ TID  GW+HTGDIG IDDD ++FIVDR+KELI
Sbjct: 444 QPGEICIRGHQIMKG---YLNNPAATAETIDKDGWLHTGDIGLIDDDDELFIVDRLKELI 503

Query: 512 KYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHV 571
           KYKGFQVAPAELEA+L+ HP I D AVV + +E AGE+P A VV + +S+ +ED++ + V
Sbjct: 504 KYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPVAFVVKSKDSELSEDDVKQFV 557

Query: 572 ASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 598
           +  V  YK++  V F ++IPK+PSGK++R+ ++ K+
Sbjct: 564 SKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

BLAST of CmUC11G217210 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 356.3 bits (913), Expect = 4.9e-98
Identity = 198/593 (33.39%), Postives = 334/593 (56.32%), Query Frame = 0

Query: 24  DLVEEEEH--IFRSQLPEVQVPDNITLPEFVLQ----NAESYAENVAFVEAVSGKAYTYR 83
           D  EE  H  IFRS+LP++ +P+++ L ++V Q    + +  +     ++  +G+  TY 
Sbjct: 18  DEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYA 77

Query: 84  EVVRDINRFSKALCSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 143
           +V  ++ R +  +  L ++ G VV+++LPN  E+A+  L +   G V +  NP     EI
Sbjct: 78  DVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEI 137

Query: 144 KKQVEVAEAKLVVTNSANFEKVKELKLPVILL------GEELI-----EGSINWHKLLEA 203
            KQ + + AK+++T     +K+  LK   +L+      G+  +     +G +++ +L +A
Sbjct: 138 AKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQA 197

Query: 204 ADRAGNDCVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLIANLCSTLSGVPREIE--G 263
            +    + +K  I   D  A+P+SSGTTG+ KGVM+TH+ L+ ++   + G    +    
Sbjct: 198 DE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTA 257

Query: 264 KVTTLGLIPFFHIYGITGICCAALRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII 323
               L  +P FHIY +  +  +A+R    ++++ RF+L   +  +   +VT  P+ PP++
Sbjct: 258 NDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVV 317

Query: 324 LALVKNPIVEEFDLSGLKLQAIMTAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFI 383
           LA +K+P  E +DLS +++  +++ AA L  E++ A   KFP     +            
Sbjct: 318 LAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQ------------ 377

Query: 384 PSNFLVMTCSTTSCFRHIRRKPSSTAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPN 443
                                     YG+TE   +  +  +  K+    K    G ++ N
Sbjct: 378 -------------------------GYGMTESGTVAKSL-AFAKNPFKTKSGACGTVIRN 437

Query: 444 LEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGY 503
            E+K +D ++G SLP+N  GEICVR   +M+G   Y  + E T+RTID  GW+HTGDIG+
Sbjct: 438 AEMKVVDTETGISLPRNKSGEICVRGHQLMKG---YLNDPEATARTIDKDGWLHTGDIGF 497

Query: 504 IDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASV 563
           +DDD ++FIVDR+KELIK+KG+QVAPAELEA+L++HPSI+DAAVV + DE A E+P A V
Sbjct: 498 VDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSIDDAAVVAMKDEVADEVPVAFV 557

Query: 564 VMAPNSKETEDEIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 598
             +  S+ TED++  +V   V HYK++++V F++ IPK+ SGK++R+ ++ K+
Sbjct: 558 ARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAVSGKILRKDLRAKL 564

BLAST of CmUC11G217210 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 352.1 bits (902), Expect = 9.2e-97
Identity = 201/570 (35.26%), Postives = 319/570 (55.96%), Query Frame = 0

Query: 32  IFRSQLPEVQVPDNITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDINRFSKALC 91
           IFRS+LP++ +P+++ L  +  +   S ++    +   +GK+YTY E      R +  L 
Sbjct: 34  IFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLIVGSTGKSYTYGETHLICRRVASGLY 93

Query: 92  SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAEAKLVVT 151
            L ++KG V++++L N AE+    +G    G V +  NP     E+ KQ++ + AKL++T
Sbjct: 94  KLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVSTTANPFYTSQELYKQLKSSGAKLIIT 153

Query: 152 NSANFEKVKEL--KLPVILLGEELIEGSINWHKLLEAADRAGNDCVKEDIKQTDLCALPF 211
           +S   +K+K L   L +I   E   E  + +  L+   D         DI   D  ALPF
Sbjct: 154 HSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI-TDDETNPFQETVDIGGDDAAALPF 213

Query: 212 SSGTTGVSKGVMLTHRNLIANLCSTLSGVPRE--IEGKVTTLGLIPFFHIYGITGICCAA 271
           SSGTTG+ KGV+LTH++LI ++   + G      ++     L ++P FHIY +  +   +
Sbjct: 214 SSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLYLKSNDVILCVLPLFHIYSLNSVLLNS 273

Query: 272 LRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSGLKLQAIM 331
           LR+   V++M +F++   ++ +    VT A +VPP+++AL KNP V  +DLS ++   ++
Sbjct: 274 LRSGATVLLMHKFEIGALLDLIQRHRVTIAALVPPLVIALAKNPTVNSYDLSSVRF--VL 333

Query: 332 TAAAPLAPEVQTAFERKFPGVDIQESIDCDRSLLLFIPSNFLVMTCSTTSCFRHIRRKPS 391
           + AAPL  E+Q +  R+ P   + +                                   
Sbjct: 334 SGAAPLGKELQDSLRRRLPQAILGQ----------------------------------- 393

Query: 392 STAYGLTEHSCITLNYGSMGKDNLTAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEIC 451
              YG+TE   +        K+ +  K  + G ++ N E+K +  ++  SL  N PGEIC
Sbjct: 394 --GYGMTEAGPVLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEIC 453

Query: 452 VRSQCVMQGTERYYKNEEETSRTIDNKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQ 511
           +R Q +M   + Y  + E TS TID +GW+HTGDIGY+D+D ++FIVDR+KE+IK+KGFQ
Sbjct: 454 IRGQQIM---KEYLNDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQ 513

Query: 512 VAPAELEAILLAHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSKETEDEIIKHVASNVAH 571
           V PAELE++L+ H SI DAAVVP  DE AGE+P A VV +  +  TE+++ ++VA  V  
Sbjct: 514 VPPAELESLLINHHSIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVF 560

Query: 572 YKKVRLVHFVDTIPKSPSGKVMRRLIKEKM 598
           YK++  V FV +IPKSPSGK++R+ +K K+
Sbjct: 574 YKRLHKVFFVASIPKSPSGKILRKDLKAKL 560

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038893074.16.7e-29189.154-coumarate--CoA ligase-like 1 [Benincasa hispida][more]
XP_022945246.13.7e-28986.454-coumarate--CoA ligase-like 1 [Cucurbita moschata][more]
KAG7012722.11.5e-28785.954-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_023541569.11.3e-28685.794-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo][more]
XP_022966799.11.1e-28585.244-coumarate--CoA ligase-like 1 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9LQ121.3e-21768.014-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=... [more]
Q7XXL22.5e-19259.594-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9... [more]
P316846.9e-10535.204-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1[more]
P316852.0e-10435.204-coumarate--CoA ligase 2 OS=Solanum tuberosum OX=4113 GN=4CL2 PE=3 SV=1[more]
I3PB372.6e-10436.014-coumarate:CoA ligase 1 OS=Petunia hybrida OX=4102 GN=4CL1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G0B41.8e-28986.454-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=... [more]
A0A6J1HUV55.3e-28685.244-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ... [more]
A0A6J1DG057.2e-28385.174-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A6J1DEE19.4e-28385.864-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1... [more]
A0A5D3CV414.8e-27984.254-coumarate--CoA ligase-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_s... [more]
Match NameE-valueIdentityDescription
AT1G62940.19.5e-21968.01acyl-CoA synthetase 5 [more]
AT3G21240.11.3e-10334.694-coumarate:CoA ligase 2 [more]
AT1G51680.12.6e-9934.034-coumarate:CoA ligase 1 [more]
AT3G21230.14.9e-9833.394-coumarate:CoA ligase 5 [more]
AT1G65060.19.2e-9735.264-coumarate:CoA ligase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Watermelon (USVL531) v1
Date Performed: 2022-01-31
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.30.300.30coord: 499..601
e-value: 1.1E-31
score: 111.5
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 388..600
NoneNo IPR availablePANTHERPTHR24096:SF3894-COUMARATE--COA LIGASE-LIKE 1coord: 24..353
coord: 388..600
NoneNo IPR availablePANTHERPTHR24096LONG-CHAIN-FATTY-ACID--COA LIGASEcoord: 24..353
NoneNo IPR availableCDDcd059044CLcoord: 42..591
e-value: 0.0
score: 654.304
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 34..351
NoneNo IPR availableSUPERFAMILY56801Acetyl-CoA synthetase-likecoord: 384..600
IPR000873AMP-dependent synthetase/ligasePFAMPF00501AMP-bindingcoord: 54..347
e-value: 8.1E-51
score: 172.9
coord: 379..503
e-value: 1.1E-33
score: 116.5
IPR042099ANL, N-terminal domainGENE3D3.40.50.12780coord: 30..358
e-value: 9.9E-82
score: 276.9
coord: 366..498
e-value: 3.9E-37
score: 129.9
IPR025110AMP-binding enzyme, C-terminal domainPFAMPF13193AMP-binding_Ccoord: 512..587
e-value: 7.4E-18
score: 65.3
IPR020845AMP-binding, conserved sitePROSITEPS00455AMP_BINDINGcoord: 207..218

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmUC11G217210.1CmUC11G217210.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046949 fatty-acyl-CoA biosynthetic process
biological_process GO:0001676 long-chain fatty acid metabolic process
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004467 long-chain fatty acid-CoA ligase activity