CmUC06G122320 (gene) Watermelon (USVL531) v1

BLAST of CmUC06G122320 vs. NCBI nr
Match: XP_038879251.1 (isoamylase 1, chloroplastic [Benincasa hispida])

HSP 1 Score: 1550.0 bits (4012), Expect = 0.0e+00
Identity = 753/852 (88.38%), Postives = 765/852 (89.79%), Query Frame = 0

Query: 1   MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
           MEL+TRSASLSSSASFLC SKL F ECRRRNSKQNVVR RRRSDIRFDLDVN  HSRMNA
Sbjct: 1   MELVTRSASLSSSASFLCASKLNFPECRRRNSKQNVVRDRRRSDIRFDLDVNWFHSRMNA 60

Query: 61  SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
           SVTRA NRGA+VGT + MAEEVEVS  NRFEVFDGTPTPFGATTR+GG+NFAIYSANAVS
Sbjct: 61  SVTRAWNRGAKVGTSIAMAEEVEVSISNRFEVFDGTPTPFGATTRDGGVNFAIYSANAVS 120

Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
           ATLCLINL DL EN VTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKF+GKFSPQEGC
Sbjct: 121 ATLCLINLSDLHENSVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFEGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
           YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT+NEEIKNFDWEGDLPLQHA
Sbjct: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTINEEIKNFDWEGDLPLQHA 240

Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
           QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF
Sbjct: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300

Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
           SYNSVLGDYKMNFWGYSTVN+FSPMIRYSSA IGNCGQDAINEFKLLIREAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKMNFWGYSTVNYFSPMIRYSSASIGNCGQDAINEFKLLIREAHKRGIEVIM 360

Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
           DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDC 420

Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
           LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNV+GNPIEGDVLTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVFGNPIEGDVLTTGSPLGNPPLIDMISN 480

Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
           DPVLQGVK                                        LIAEAWDAGGLY
Sbjct: 481 DPVLQGVK----------------------------------------LIAEAWDAGGLY 540

Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
           QVGTFPHWGVWSEWNG+YRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600

Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
           ICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660

Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
           FFVCLMVSQ                           LNYFRWDK+EESSSDFHRFCSLMT
Sbjct: 661 FFVCLMVSQGVPLILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESSSDFHRFCSLMT 720

Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
           TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL
Sbjct: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780

Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 826
           PVTV LPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQY HFLDANLYPMLSYSSI
Sbjct: 781 PVTVTLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYVHFLDANLYPMLSYSSI 812

BLAST of CmUC06G122320 vs. NCBI nr
Match: XP_008453530.1 (PREDICTED: isoamylase 1, chloroplastic [Cucumis melo] >KAA0058172.1 isoamylase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 734/853 (86.05%), Postives = 755/853 (88.51%), Query Frame = 0

Query: 1   MELITRS-ASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMN 60
           MEL+TRS ASL SSASFLC S L F ECRRRN KQNVVRGRRR+DI FDLD N IHS +N
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60

Query: 61  ASVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAV 120
               RARNRGAEVGT V +AEE E+SNLNRFEVFDGTPTPFGAT R+ GINFAIYSANAV
Sbjct: 61  ----RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAV 120

Query: 121 SATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
           SATLCL++L DLQEN VTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG
Sbjct: 121 SATLCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQH 240
           CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT NE+IKNFDWEGDLPLQH
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQH 240

Query: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
           AQKDLIIYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
           FSYNSVLGDYKMNFWGYSTVN+FSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360

Query: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVD 420
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420

Query: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
           CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGL 540
           NDPVLQGVK                                        LIAEAWDAGGL
Sbjct: 481 NDPVLQGVK----------------------------------------LIAEAWDAGGL 540

Query: 541 YQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
           YQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKP NSIN
Sbjct: 541 YQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPCNSIN 600

Query: 601 FICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
           F+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR
Sbjct: 601 FVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660

Query: 661 NFFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLM 720
           NFFVCLMVSQ                           LNYFRWDK+EES+SDFHRFCSLM
Sbjct: 661 NFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSLM 720

Query: 721 TTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
           TTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH
Sbjct: 721 TTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780

Query: 781 LPVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSS 826
           LPVTV LPERPGYRWDPLVDTGK SPFDFLT+D+PER++AIQQYAHFLDANLYPMLSYSS
Sbjct: 781 LPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQYAHFLDANLYPMLSYSS 809

BLAST of CmUC06G122320 vs. NCBI nr
Match: XP_004149676.1 (isoamylase 1, chloroplastic [Cucumis sativus] >KAE8653176.1 hypothetical protein Csa_019536 [Cucumis sativus])

HSP 1 Score: 1503.4 bits (3891), Expect = 0.0e+00
Identity = 735/854 (86.07%), Postives = 756/854 (88.52%), Query Frame = 0

Query: 1   MELITRS-ASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMN 60
           MEL+T S ASL SSASFLC S L F ECRRRNSKQNVVRGRRRSDI FDLD N IHS +N
Sbjct: 1   MELVTHSAASLFSSASFLCVSNLSFPECRRRNSKQNVVRGRRRSDICFDLDANWIHSSLN 60

Query: 61  ASVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAV 120
               RARNRGAEV T V +AEEVEVSNLNRFEVFDGTPTPFGAT RE GINFAIYSANAV
Sbjct: 61  ----RARNRGAEVRTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAV 120

Query: 121 SATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
           SATLCLI+L DLQENRVTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG
Sbjct: 121 SATLCLIHLSDLQENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQH 240
           CYFD SRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT NE+IK+FDWEGDLP+QH
Sbjct: 181 CYFDSSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQH 240

Query: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
           AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELG+NCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
           FSYNSVLGDYKMNFWGYSTVN+FSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360

Query: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVD 420
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420

Query: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
           CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYG+PIEGD+LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGSPIEGDILTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGL 540
           NDPVLQGVK                                        LIAEAWDAGGL
Sbjct: 481 NDPVLQGVK----------------------------------------LIAEAWDAGGL 540

Query: 541 YQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
           YQVGTFPHWGVWSEWNGKYRD++RQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN
Sbjct: 541 YQVGTFPHWGVWSEWNGKYRDVIRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600

Query: 601 FICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
           FICAHDGF+LADLVTYNSK NLANGE+NNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR
Sbjct: 601 FICAHDGFTLADLVTYNSKQNLANGEENNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660

Query: 661 NFFVCLMVSQ---------------------------LNYFRWDKLEESSS-DFHRFCSL 720
           NFFVCLMVSQ                           LNYFRWDK+EES+S DFHRFCSL
Sbjct: 661 NFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSFDFHRFCSL 720

Query: 721 MTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNAS 780
           MTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNAS
Sbjct: 721 MTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNAS 780

Query: 781 HLPVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYS 826
           HLPVTV LPERPGYRWDPLVDTGK SPFDFLT+D+P+RDVAIQQYAHFLDAN YPMLSYS
Sbjct: 781 HLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPKRDVAIQQYAHFLDANYYPMLSYS 810

BLAST of CmUC06G122320 vs. NCBI nr
Match: XP_022134724.1 (isoamylase 1, chloroplastic [Momordica charantia])

HSP 1 Score: 1448.0 bits (3747), Expect = 0.0e+00
Identity = 702/851 (82.49%), Postives = 737/851 (86.60%), Query Frame = 0

Query: 1   MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
           MEL+TRS SL  SA FLC  K RF ECRRR+SK N VR RRRSDI  DLD N I+SRM  
Sbjct: 1   MELVTRSTSLCWSACFLCAPKSRFPECRRRSSKNNAVRSRRRSDIHMDLDANWINSRMKM 60

Query: 61  SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
           S+TRA NRGAEVGT V MAEEVEVSNL+RFEVF+G P P GAT R+GG+NFA+YS NAVS
Sbjct: 61  SLTRASNRGAEVGTSVAMAEEVEVSNLHRFEVFEGDPAPLGATARDGGMNFAVYSGNAVS 120

Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
           ATLCL+NLFDLQENRVT+E+ LDPLTNKTGDVWHVFLKG FTEMLYGYKFDG+FSPQ+GC
Sbjct: 121 ATLCLMNLFDLQENRVTKEVTLDPLTNKTGDVWHVFLKGGFTEMLYGYKFDGEFSPQDGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
           YFDPSRILLDPYAKAVISRGEFGA GP++NCWPQMAGMVPTV +E   FDWEGDLPLQ+ 
Sbjct: 181 YFDPSRILLDPYAKAVISRGEFGAPGPDNNCWPQMAGMVPTVIDE---FDWEGDLPLQYP 240

Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
           QKDLIIYEMHVRGFTRHESS SEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF
Sbjct: 241 QKDLIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300

Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
           SYNSVLGDYKMNFWGYSTV FFSPMIRYSSAGI  CGQDAINEFKLLIREAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLIREAHKRGIEVIM 360

Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
           DVVFNHTAEGNENGP  SFRGVDN+VYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420

Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
           LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480

Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
           DPVLQGVK                                        LIAEAWDAGGLY
Sbjct: 481 DPVLQGVK----------------------------------------LIAEAWDAGGLY 540

Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
           QVGTFPHWGVWSEWNG+YRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600

Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
           ICAHDGF+LADLVTYN+KHNLANGEDNNDGE+HNNSWNCGQEGEFVS+SVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFVSMSVKKLRKRQMRN 660

Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
           FFVCLMVSQ                           +NYFRWDKLEESSSDFHRFCSL+T
Sbjct: 661 FFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWDKLEESSSDFHRFCSLVT 720

Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
           TFR+ECESLGLNDFPT ERLQWHG+SPGKPDW+EASRFVAFTLVDSVK E+YIAFNA+HL
Sbjct: 721 TFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFVAFTLVDSVKGEIYIAFNANHL 780

Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 825
           PVTV LPERPGYRW+PLVDTGKISPFDFL+SDLPERDVAI+QYAHFLD NLYPML YSSI
Sbjct: 781 PVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQYAHFLDNNLYPMLCYSSI 808

BLAST of CmUC06G122320 vs. NCBI nr
Match: XP_022932124.1 (isoamylase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1443.7 bits (3736), Expect = 0.0e+00
Identity = 704/852 (82.63%), Postives = 735/852 (86.27%), Query Frame = 0

Query: 1   MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
           MEL+TRSASLSSS SFL  S   F E RRR+SK NV+  RRRSDI  DL V+ IHSR+N 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60

Query: 61  SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
           S TRA +RGAEV T VT+AEEVE+SN NRFEVF+GTPTPFGAT R+GGINFAIYS NAVS
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
           ATLCLINLFDLQ+ RV QEI LDPL N+TGDVWHVFLKGDFT+MLYGYKF GKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
           YFDPSRILLDPYAKAVISR EFGALGP+DNCWPQMAGMVPTV++E   FDWEGDLPLQH 
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQHP 240

Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
           QKDLIIYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY+
Sbjct: 241 QKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYY 300

Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
           SYNSVLGDYK+NFWGYSTV F SPMIRYSSAGI NCGQDAINEFKLLI+EAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIM 360

Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
           DVVFNHTAEGNENGP  SFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420

Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
           LRYWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISN 480

Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
           DP+LQGVK                                        LIAEAWDAGGLY
Sbjct: 481 DPILQGVK----------------------------------------LIAEAWDAGGLY 540

Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
           QVGTFPHW VWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600

Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
           ICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660

Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
           FFVCLMVSQ                           +NYFRWDK+EESSSDFHRFC LMT
Sbjct: 661 FFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEESSSDFHRFCRLMT 720

Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
           TFRYECESLGLNDFPT +RLQWHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHL
Sbjct: 721 TFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHL 780

Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 826
           PVTV LPERPGY+W+PLVDTGKISPFDFLTSDL ERDVAIQQYAHFLDANLYPMLSYSSI
Sbjct: 781 PVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQYAHFLDANLYPMLSYSSI 809

BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match: O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 534/784 (68.11%), Postives = 623/784 (79.46%), Query Frame = 0

Query: 65  ARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVSATLC 124
           A++R +     + + E+   S  +RF + DG P+PFG T R+ G+NF++YS N+VSAT+C
Sbjct: 44  AKDRRSNEAENIAVVEKPLKS--DRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATIC 103

Query: 125 LINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDP 184
           LI+L DL++N+VT+EI+LDP  N+TG VWHVFL+GDF +MLYGY+FDGKFSP+EG Y+D 
Sbjct: 104 LISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDS 163

Query: 185 SRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDL 244
           S ILLDPYAKA+ISR EFG LGP+DNCWPQMA MVPT  EE   FDWEGD+ L+  QKDL
Sbjct: 164 SNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEE---FDWEGDMHLKLPQKDL 223

Query: 245 IIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNS 304
           +IYEMHVRGFTRHESS+ EFPGTY G+ EKLD+LKELG+NCIELMPCHEFNELEY+SYN+
Sbjct: 224 VIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNT 283

Query: 305 VLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVF 364
           +LGD+++NFWGYST+ FFSPMIRY+SA   N    AINEFK+L++EAHKRGIEVIMDVV 
Sbjct: 284 ILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVL 343

Query: 365 NHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYW 424
           NHTAEGNE GP  SFRGVDN+VYYMLAP GEFYNYSGCGNTFNCNHP VR FI+DCLRYW
Sbjct: 344 NHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYW 403

Query: 425 VTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVL 484
           VTEMHVDGFRFDL SIM+R SSLWDA NVYG  +EGD+LTTG+P+  PP+IDMISNDP+L
Sbjct: 404 VTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPIL 463

Query: 485 QGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGT 544
           +GVK                                        LIAEAWDAGGLYQVG 
Sbjct: 464 RGVK----------------------------------------LIAEAWDAGGLYQVGM 523

Query: 545 FPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAH 604
           FPHWG+WSEWNGK+RD+VRQFIKGTDGFSGAFAECLCGSPNLYQ GGRKPW+SINFICAH
Sbjct: 524 FPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAH 583

Query: 605 DGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVC 664
           DGF+LADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F SISVK+LRKRQMRNFFV 
Sbjct: 584 DGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVS 643

Query: 665 LMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMTTFRY 724
           LMVSQ                           +NYFRWDK EE+ SDF RFC ++  FR 
Sbjct: 644 LMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRD 703

Query: 725 ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTV 784
           ECESLGLNDFPT +RLQWHG +P  P+W+E SRFVAF+LVDSVK+E+Y+AFN SHL   V
Sbjct: 704 ECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSVKKEIYVAFNTSHLATLV 763

Query: 785 MLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSIILLL 822
            LP RPGYRW+P VDT K SP+D +T DLPER+ A++QY HFLDAN+YPMLSYSSIILLL
Sbjct: 764 SLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIILLL 781

BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match: D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)

HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 527/823 (64.03%), Postives = 604/823 (73.39%), Query Frame = 0

Query: 28  RRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNASVTRARNRGAEVGTK----VTMAEEVE 87
           RRRN+  +     RR  +R       + S +   V      G E   +    V M E   
Sbjct: 35  RRRNAAFSAGNAGRRVGLR-----RSVASAVEVGVGEDEEEGVEEEEEEVEAVVMPERYA 94

Query: 88  VSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVSATLCLINLFDLQENRVTQEIRLD 147
           +    R  V  G P P GAT  +GG+NFA+YSA A +A+LCL    DL+ + VT+E+ LD
Sbjct: 95  LGGACR--VLAGMPAPLGATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLD 154

Query: 148 PLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFG 207
           PL N+TG+VWHVF++G+   MLYGY+FDG F+P  G YFD S +++DPYAKAVISRGE+G
Sbjct: 155 PLFNRTGNVWHVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYG 214

Query: 208 ALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQSE 267
             GP  +CWPQMAGM+P        FDW+GDLPL++ QKDL+IYEMH+RGFT+H SS  E
Sbjct: 215 VPGPGGDCWPQMAGMIPL---PYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNVE 274

Query: 268 FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNFFS 327
            PGTY+G + KLDYLKELGVNC+ELMPCHEFNELEYFS +S     KMNFWGYST+NFFS
Sbjct: 275 HPGTYIGAISKLDYLKELGVNCVELMPCHEFNELEYFSCSS-----KMNFWGYSTINFFS 334

Query: 328 PMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVD 387
           PMIRYSS GI NCG+DAINEFK  +REAHKRGIEVIMDVVFNHTAEGNE GP +SFRG+D
Sbjct: 335 PMIRYSSGGIRNCGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGID 394

Query: 388 NNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTR 447
           N+ YYMLAP GEFYNYSGCGNTFNCNHP VR FIVDCLRYWVTEMHVDGFRFDLASIMTR
Sbjct: 395 NSTYYMLAPKGEFYNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTR 454

Query: 448 GSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLH 507
           G SLWD VNVYG+P+EGD+ TTG+PL  PPLIDMISNDP+L  VK               
Sbjct: 455 GCSLWDPVNVYGSPVEGDMTTTGTPLATPPLIDMISNDPILGDVK--------------- 514

Query: 508 GQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVR 567
                                    LIAEAWDAGGLYQVG FPHW +WSEWNGKYRDIVR
Sbjct: 515 -------------------------LIAEAWDAGGLYQVGQFPHWKIWSEWNGKYRDIVR 574

Query: 568 QFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLAN 627
           QFIKGTDGF+G FAECLCGSP+LYQ GGRKPW+SINF+CAHDGF+LADLVTYN K+N +N
Sbjct: 575 QFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKKYNSSN 634

Query: 628 GEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQ-------------- 687
           GEDN DGENHN SWNCG+EGEF  +SVK+LRKRQMRNFFV LMVSQ              
Sbjct: 635 GEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGDEYGHT 694

Query: 688 -------------LNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWH 747
                        +NYFRWDK EE SSD  RFCSLMT FR +CESLGL DFPT +RL WH
Sbjct: 695 KGGNNNTYCHDHYVNYFRWDKKEE-SSDLQRFCSLMTKFRKQCESLGLADFPTAQRLHWH 754

Query: 748 GHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTGKI 807
           GH PGKPDW+E SRFVAF+  D  K E+Y+AFNASHLP  V LPERPGYRW+PLVDTGK 
Sbjct: 755 GHQPGKPDWSETSRFVAFSTKDETKGEIYVAFNASHLPAVVGLPERPGYRWEPLVDTGKP 801

Query: 808 SPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSIILLLSP 820
           +P+DFLT DLP+R  A+  ++HFL++NLYPMLSYSSIIL L P
Sbjct: 815 APYDFLTDDLPDRAHAVHLFSHFLNSNLYPMLSYSSIILELQP 801

BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match: Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)

HSP 1 Score: 542.7 bits (1397), Expect = 7.0e-153
Identity = 321/777 (41.31%), Postives = 425/777 (54.70%), Query Frame = 0

Query: 57  RMNASVTRARNRGAEVGTKVTM----------AEEVEVSNLNR---FEVFDGTPTPFGAT 116
           +++  VTRAR    ++  + T+            E E S +     F+V  G  +P G +
Sbjct: 37  KLSRRVTRARIFSRKIKDRSTLKVTCRRAHERVVEEEASTMTETKLFKVSSGEVSPLGVS 96

Query: 117 TREGGINFAIYSANAVSATLCLI---NLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKG- 176
             + GINFA++S NA S TLCL    +  D  ++    E+ LDP  NKTGD WH+ ++  
Sbjct: 97  QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDL 156

Query: 177 DFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMV 236
               +LYGY+ DG    Q+G  FD S +LLDPYAK V     FG      +   + A   
Sbjct: 157 PLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFG------DSSQKFAQFY 216

Query: 237 PTVNEEIKNFDWEGDLPLQH-AQKDLIIYEMHVRGFTRHESSQSE--FPGTYLGLVEKLD 296
            T + E   FDW  D    +  +KDL+IYEM+VR FT  ESS  +    G+YLG +EK+ 
Sbjct: 217 GTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIP 276

Query: 297 YLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNC 356
           +L++LG+N +EL+P  EF+ELE     S   D+ +N WGYSTVNFF+PM RY+S G G+ 
Sbjct: 277 HLQDLGINAVELLPVFEFDELE-LQRRSNPRDHMVNTWGYSTVNFFAPMSRYAS-GEGD- 336

Query: 357 GQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGP-TISFRGVDNNVYYMLAPMGE 416
              A  EFK +++  H  GIEVI+DVV+NHT E ++  P T SFRG+DN VYYML P  +
Sbjct: 337 PIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQ 396

Query: 417 FYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYG 476
             N+SGCGNT NCNHP V   I+D LR+WVTE HVDGFRFDLAS++ R            
Sbjct: 397 LLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRA----------- 456

Query: 477 NPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEAL 536
                   T GSPL  PPLI  I+ D VL   K+                          
Sbjct: 457 --------TDGSPLSAPPLIRAIAKDSVLSRCKI-------------------------- 516

Query: 537 CSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGA 596
                         IAE WD GGLY VG FP+W  W+EWNG YRD VR+FIKG  G  G+
Sbjct: 517 --------------IAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGS 576

Query: 597 FAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNN 656
           FA  + GS +LYQ   RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE  NDG N N+
Sbjct: 577 FATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNH 636

Query: 657 SWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQ-------------------------- 716
           SWNCG EGE     +K LR RQM+NF + LM+SQ                          
Sbjct: 637 SWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDT 696

Query: 717 -LNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEA 776
            LN F+W +L+    +  RF S +  FR+    L   +F T   + WH     + +W  +
Sbjct: 697 SLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNS 740

Query: 777 -SRFVAFTLVDSV-KREVYIAFNASHLPVTVMLPE-RPGYRWDPLVDTGKISPFDFL 783
            S+F+AFTL D +  R++Y+AFNA    V  ++P+  PG +W  + DT   SP DF+
Sbjct: 757 ESKFLAFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFV 740

BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match: B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.3e-146
Identity = 319/798 (39.97%), Postives = 424/798 (53.13%), Query Frame = 0

Query: 63  TRARNRGAEVGTKVTMAEEVEVSNLNR-FEVFDGTPTPFGATTREGGINFAIYSANAVSA 122
           T AR +    G  +T      +S+    F+   G   P G +  EGG+NFA++S +A S 
Sbjct: 70  TTARAQNGNAGRSMTEERGCTMSDTEMPFKYSSGKAFPLGVSQVEGGLNFALFSQHASSV 129

Query: 123 TLCLI---NLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKG-DFTEMLYGYKFDGKFSPQ 182
            LCL       + ++     E  LD   NKTGD+WHV ++G   + +LYGY+  G     
Sbjct: 130 ILCLKLPGRGTEDEKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWD 189

Query: 183 EGCYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPL 242
           +G  FD S +LLDPYAK V  R  FG      +   Q  G   T + +   FDW  D  L
Sbjct: 190 QGHRFDSSTVLLDPYAKLVSGRKYFGVAEEKSS---QHFG---TYDFDSSPFDWGDDYRL 249

Query: 243 QH-AQKDLIIYEMHVRGFTRHESS--QSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEF 302
            +  + DL+IYEM+VR FT  ESS   S   G+YLGL++K+ +L ELGVN +EL+P  E+
Sbjct: 250 PNLPEADLVIYEMNVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEY 309

Query: 303 NELEYFSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKR 362
           +ELE+  Y +   D+ +N WGYST+NFF+PM RY+SAG G     A  E K +++E HK 
Sbjct: 310 DELEFKRYPNP-RDHMVNTWGYSTINFFAPMSRYASAGGGPVA--ASKELKQMVKELHKA 369

Query: 363 GIEVIMDVVFNHTAEGNENGPTI-SFRGVDNNVYYM--LAPMGEFYNYSGCGNTFNCNHP 422
           GIEVI+DVV+NHT E ++  P + SFRG+DN VYYM  L    E  N+SGCGNT NCNHP
Sbjct: 370 GIEVILDVVYNHTNEADDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHP 429

Query: 423 AVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGN 482
            V+  I+D LR+WV E H+DGFRFDLAS++ RG                     G PL  
Sbjct: 430 VVKELILDSLRHWVEEYHIDGFRFDLASVLCRGPD-------------------GCPLDA 489

Query: 483 PPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIA 542
           PPLI  I+ D VL   K+                                        IA
Sbjct: 490 PPLIKEIAKDAVLSRCKI----------------------------------------IA 549

Query: 543 EAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGG 602
           E WD GGLY VG FP+W  W+EWNGKYRD +R+FIKG  G  G FA  + GS +LYQ   
Sbjct: 550 EPWDCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNE 609

Query: 603 RKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVK 662
           RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE   DG N N SWNCG EGE   ++V 
Sbjct: 610 RKPYHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVL 669

Query: 663 KLRKRQMRNFFVCLMVSQ---------------------------LNYFRWDKLEESSSD 722
            LR RQM+NF V LM+SQ                           +N F+W++LE+    
Sbjct: 670 SLRSRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDG 729

Query: 723 FHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDW-TEASRFVAFTLVD-SVKR 782
             RF S M  FR+    L  + F     + WH     +  W  + S+F+AFT+ D +   
Sbjct: 730 HFRFFSEMIKFRHSNPILRRDRFLNKNDVTWH-----EDCWENQESKFLAFTVHDHNSGG 782

Query: 783 EVYIAFNASHLPVTVMLPERPGYR-WDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLD 820
           ++Y+AFNA    V  ++P  P ++ W+ +VDT   SP D +   +P              
Sbjct: 790 DIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEGVP------------FT 782

BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match: P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)

HSP 1 Score: 404.1 bits (1037), Expect = 3.9e-111
Identity = 244/596 (40.94%), Postives = 314/596 (52.68%), Query Frame = 0

Query: 92  VFDGTPTPFGATTREGGINFAIYSANAVSATLCLINLFDLQENRVTQEIRLDPLTNKTGD 151
           V+ G   P GAT    G NF+++S  A    LCLI+     E+ V   I   PL    G 
Sbjct: 19  VWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCLID-----EDGVESRI---PLDEVDGY 78

Query: 152 VWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFG-------- 211
           VWH +L        YG++  G F P  G   DPS++LLDPY K+      FG        
Sbjct: 79  VWHAYLPNITPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTFGQALYSYDV 138

Query: 212 -ALGPNDNCWPQMAGMVPTVNEEIKN--FDWEGDLPLQHAQKDLIIYEMHVRGFTR-HES 271
            A+ P D+  P +  +  T+   + N  FDW  D   +    + +IYE HV+G T+ H S
Sbjct: 139 NAVDP-DSTPPMVDSLGHTMTSVVINPFFDWAYDRSPRTPYHETVIYEAHVKGMTQTHPS 198

Query: 272 SQSEFPGTYLGLVEK--LDYLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKM-NFWGY 331
              E  GTY GL     +D+L EL V  +ELMP H+      F ++S L D  + N+WGY
Sbjct: 199 IPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQ------FLHDSRLLDLGLRNYWGY 258

Query: 332 STVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPT 391
           +T  FF+P  +Y+S         A+ EFK ++R  H+ GIEVI+DVV+NHTAEGN  GPT
Sbjct: 259 NTFGFFAPHHQYAST---RQAGSAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNHLGPT 318

Query: 392 ISFRGVDNNVYYMLAPMG-EFY-NYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFR 451
           I+FRG+DN  YY L      FY +++G GN+ N  HP     I+D LRYWV EMHVDGFR
Sbjct: 319 INFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMHVDGFR 378

Query: 452 FDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNS 511
           FDLAS + R     D ++ +                     D++  DPV+  VK      
Sbjct: 379 FDLASTLARELHDVDRLSAF--------------------FDLVQQDPVVSQVK------ 438

Query: 512 SLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAG-GLYQVGTFPHWGVWSE 571
                                             LIAE WD G G YQVG FP  G+W+E
Sbjct: 439 ----------------------------------LIAEPWDVGEGGYQVGNFP--GLWTE 498

Query: 572 WNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLV 631
           WNGKYRD VR + +G     G FA  L GS +LY+  GR+P  SINF+ AHDGF+L DLV
Sbjct: 499 WNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLNDLV 534

Query: 632 TYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQ 670
           +YN KHN ANGE+N DGE++N SWNCG EG      +  LR RQMRN +  LMVSQ
Sbjct: 559 SYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQMRNMWATLMVSQ 534

BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match: A0A5A7UQM5 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 PE=4 SV=1)

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 734/853 (86.05%), Postives = 755/853 (88.51%), Query Frame = 0

Query: 1   MELITRS-ASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMN 60
           MEL+TRS ASL SSASFLC S L F ECRRRN KQNVVRGRRR+DI FDLD N IHS +N
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60

Query: 61  ASVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAV 120
               RARNRGAEVGT V +AEE E+SNLNRFEVFDGTPTPFGAT R+ GINFAIYSANAV
Sbjct: 61  ----RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAV 120

Query: 121 SATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
           SATLCL++L DLQEN VTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG
Sbjct: 121 SATLCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQH 240
           CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT NE+IKNFDWEGDLPLQH
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQH 240

Query: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
           AQKDLIIYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
           FSYNSVLGDYKMNFWGYSTVN+FSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360

Query: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVD 420
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420

Query: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
           CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGL 540
           NDPVLQGVK                                        LIAEAWDAGGL
Sbjct: 481 NDPVLQGVK----------------------------------------LIAEAWDAGGL 540

Query: 541 YQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
           YQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKP NSIN
Sbjct: 541 YQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPCNSIN 600

Query: 601 FICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
           F+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR
Sbjct: 601 FVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660

Query: 661 NFFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLM 720
           NFFVCLMVSQ                           LNYFRWDK+EES+SDFHRFCSLM
Sbjct: 661 NFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSLM 720

Query: 721 TTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
           TTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH
Sbjct: 721 TTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780

Query: 781 LPVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSS 826
           LPVTV LPERPGYRWDPLVDTGK SPFDFLT+D+PER++AIQQYAHFLDANLYPMLSYSS
Sbjct: 781 LPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQYAHFLDANLYPMLSYSS 809

BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match: A0A1S3BVW8 (isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1)

HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 734/853 (86.05%), Postives = 755/853 (88.51%), Query Frame = 0

Query: 1   MELITRS-ASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMN 60
           MEL+TRS ASL SSASFLC S L F ECRRRN KQNVVRGRRR+DI FDLD N IHS +N
Sbjct: 1   MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60

Query: 61  ASVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAV 120
               RARNRGAEVGT V +AEE E+SNLNRFEVFDGTPTPFGAT R+ GINFAIYSANAV
Sbjct: 61  ----RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAV 120

Query: 121 SATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
           SATLCL++L DLQEN VTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG
Sbjct: 121 SATLCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180

Query: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQH 240
           CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT NE+IKNFDWEGDLPLQH
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQH 240

Query: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
           AQKDLIIYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300

Query: 301 FSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
           FSYNSVLGDYKMNFWGYSTVN+FSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360

Query: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVD 420
           MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420

Query: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
           CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 480

Query: 481 NDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGL 540
           NDPVLQGVK                                        LIAEAWDAGGL
Sbjct: 481 NDPVLQGVK----------------------------------------LIAEAWDAGGL 540

Query: 541 YQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
           YQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKP NSIN
Sbjct: 541 YQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPCNSIN 600

Query: 601 FICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
           F+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR
Sbjct: 601 FVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660

Query: 661 NFFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLM 720
           NFFVCLMVSQ                           LNYFRWDK+EES+SDFHRFCSLM
Sbjct: 661 NFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSLM 720

Query: 721 TTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
           TTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH
Sbjct: 721 TTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780

Query: 781 LPVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSS 826
           LPVTV LPERPGYRWDPLVDTGK SPFDFLT+D+PER++AIQQYAHFLDANLYPMLSYSS
Sbjct: 781 LPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQYAHFLDANLYPMLSYSS 809

BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match: A0A6J1BZK5 (isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 SV=1)

HSP 1 Score: 1448.0 bits (3747), Expect = 0.0e+00
Identity = 702/851 (82.49%), Postives = 737/851 (86.60%), Query Frame = 0

Query: 1   MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
           MEL+TRS SL  SA FLC  K RF ECRRR+SK N VR RRRSDI  DLD N I+SRM  
Sbjct: 1   MELVTRSTSLCWSACFLCAPKSRFPECRRRSSKNNAVRSRRRSDIHMDLDANWINSRMKM 60

Query: 61  SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
           S+TRA NRGAEVGT V MAEEVEVSNL+RFEVF+G P P GAT R+GG+NFA+YS NAVS
Sbjct: 61  SLTRASNRGAEVGTSVAMAEEVEVSNLHRFEVFEGDPAPLGATARDGGMNFAVYSGNAVS 120

Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
           ATLCL+NLFDLQENRVT+E+ LDPLTNKTGDVWHVFLKG FTEMLYGYKFDG+FSPQ+GC
Sbjct: 121 ATLCLMNLFDLQENRVTKEVTLDPLTNKTGDVWHVFLKGGFTEMLYGYKFDGEFSPQDGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
           YFDPSRILLDPYAKAVISRGEFGA GP++NCWPQMAGMVPTV +E   FDWEGDLPLQ+ 
Sbjct: 181 YFDPSRILLDPYAKAVISRGEFGAPGPDNNCWPQMAGMVPTVIDE---FDWEGDLPLQYP 240

Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
           QKDLIIYEMHVRGFTRHESS SEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF
Sbjct: 241 QKDLIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300

Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
           SYNSVLGDYKMNFWGYSTV FFSPMIRYSSAGI  CGQDAINEFKLLIREAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLIREAHKRGIEVIM 360

Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
           DVVFNHTAEGNENGP  SFRGVDN+VYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420

Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
           LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480

Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
           DPVLQGVK                                        LIAEAWDAGGLY
Sbjct: 481 DPVLQGVK----------------------------------------LIAEAWDAGGLY 540

Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
           QVGTFPHWGVWSEWNG+YRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600

Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
           ICAHDGF+LADLVTYN+KHNLANGEDNNDGE+HNNSWNCGQEGEFVS+SVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFVSMSVKKLRKRQMRN 660

Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
           FFVCLMVSQ                           +NYFRWDKLEESSSDFHRFCSL+T
Sbjct: 661 FFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWDKLEESSSDFHRFCSLVT 720

Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
           TFR+ECESLGLNDFPT ERLQWHG+SPGKPDW+EASRFVAFTLVDSVK E+YIAFNA+HL
Sbjct: 721 TFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFVAFTLVDSVKGEIYIAFNANHL 780

Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 825
           PVTV LPERPGYRW+PLVDTGKISPFDFL+SDLPERDVAI+QYAHFLD NLYPML YSSI
Sbjct: 781 PVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQYAHFLDNNLYPMLCYSSI 808

BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match: A0A6J1EW54 (isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 SV=1)

HSP 1 Score: 1443.7 bits (3736), Expect = 0.0e+00
Identity = 704/852 (82.63%), Postives = 735/852 (86.27%), Query Frame = 0

Query: 1   MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
           MEL+TRSASLSSS SFL  S   F E RRR+SK NV+  RRRSDI  DL V+ IHSR+N 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60

Query: 61  SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
           S TRA +RGAEV T VT+AEEVE+SN NRFEVF+GTPTPFGAT R+GGINFAIYS NAVS
Sbjct: 61  SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
           ATLCLINLFDLQ+ RV QEI LDPL N+TGDVWHVFLKGDFT+MLYGYKF GKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
           YFDPSRILLDPYAKAVISR EFGALGP+DNCWPQMAGMVPTV++E   FDWEGDLPLQH 
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQHP 240

Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
           QKDLIIYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY+
Sbjct: 241 QKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYY 300

Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
           SYNSVLGDYK+NFWGYSTV F SPMIRYSSAGI NCGQDAINEFKLLI+EAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIM 360

Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
           DVVFNHTAEGNENGP  SFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420

Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
           LRYWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISN 480

Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
           DP+LQGVK                                        LIAEAWDAGGLY
Sbjct: 481 DPILQGVK----------------------------------------LIAEAWDAGGLY 540

Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
           QVGTFPHW VWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600

Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
           ICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660

Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
           FFVCLMVSQ                           +NYFRWDK+EESSSDFHRFC LMT
Sbjct: 661 FFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEESSSDFHRFCRLMT 720

Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
           TFRYECESLGLNDFPT +RLQWHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHL
Sbjct: 721 TFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHL 780

Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 826
           PVTV LPERPGY+W+PLVDTGKISPFDFLTSDL ERDVAIQQYAHFLDANLYPMLSYSSI
Sbjct: 781 PVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQYAHFLDANLYPMLSYSSI 809

BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match: A0A6J1ICQ3 (isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=1)

HSP 1 Score: 1440.2 bits (3727), Expect = 0.0e+00
Identity = 704/852 (82.63%), Postives = 734/852 (86.15%), Query Frame = 0

Query: 1   MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
           MEL+TRSASLSSS SFL  S   F E RRR+SK+NV+  RRRSDI  DLDV+ IHSR+N 
Sbjct: 1   MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKRNVIPIRRRSDIHLDLDVDWIHSRINV 60

Query: 61  SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
           S TRA +RGAEV T VTMAEEVE+SN NRFEVF+GTPTPFGAT R+GGINFAIYS NAVS
Sbjct: 61  SETRASSRGAEVRTSVTMAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120

Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
           ATLCLINLFDLQ+ RV QEI LDPLTN+TGDVWHVFLKGDFT+MLYGYKF GKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180

Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
           YFDPSRILLDPYAKAVISR EFGALGP+DNCWPQMAGMVPTV++E   FDWEGDLPLQH 
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQHP 240

Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
           QKDLIIYEMHVRGFTRHESSQS  PGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY+
Sbjct: 241 QKDLIIYEMHVRGFTRHESSQSGSPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYY 300

Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
           SYNSVLGDYK+NFWGYSTV F SPMIRYSSAGI NCGQDAINEFKLLI+EAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIM 360

Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
           DVVFNHTAEGNENGP  SFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420

Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
           LRYWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISN 480

Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
           DP+LQGVK                                        LIAEAWDAGGLY
Sbjct: 481 DPILQGVK----------------------------------------LIAEAWDAGGLY 540

Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
           QVGTFPHW VWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600

Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
           ICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660

Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
           FFVCLMVSQ                           +NYF WDK+EESSSDFHRFC LMT
Sbjct: 661 FFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFLWDKMEESSSDFHRFCRLMT 720

Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
           TFRYECESLGLNDFPT +RLQWHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHL
Sbjct: 721 TFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHL 780

Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 826
           PVTV LPER GYRW+PLVDTGKISPFDFLTSDL ERDVAIQQYAHFLDANLYPMLSYSSI
Sbjct: 781 PVTVTLPERLGYRWEPLVDTGKISPFDFLTSDLEERDVAIQQYAHFLDANLYPMLSYSSI 809

BLAST of CmUC06G122320 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 534/784 (68.11%), Postives = 623/784 (79.46%), Query Frame = 0

Query: 65  ARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVSATLC 124
           A++R +     + + E+   S  +RF + DG P+PFG T R+ G+NF++YS N+VSAT+C
Sbjct: 44  AKDRRSNEAENIAVVEKPLKS--DRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATIC 103

Query: 125 LINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDP 184
           LI+L DL++N+VT+EI+LDP  N+TG VWHVFL+GDF +MLYGY+FDGKFSP+EG Y+D 
Sbjct: 104 LISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDS 163

Query: 185 SRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDL 244
           S ILLDPYAKA+ISR EFG LGP+DNCWPQMA MVPT  EE   FDWEGD+ L+  QKDL
Sbjct: 164 SNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEE---FDWEGDMHLKLPQKDL 223

Query: 245 IIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNS 304
           +IYEMHVRGFTRHESS+ EFPGTY G+ EKLD+LKELG+NCIELMPCHEFNELEY+SYN+
Sbjct: 224 VIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNT 283

Query: 305 VLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVF 364
           +LGD+++NFWGYST+ FFSPMIRY+SA   N    AINEFK+L++EAHKRGIEVIMDVV 
Sbjct: 284 ILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVL 343

Query: 365 NHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYW 424
           NHTAEGNE GP  SFRGVDN+VYYMLAP GEFYNYSGCGNTFNCNHP VR FI+DCLRYW
Sbjct: 344 NHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYW 403

Query: 425 VTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVL 484
           VTEMHVDGFRFDL SIM+R SSLWDA NVYG  +EGD+LTTG+P+  PP+IDMISNDP+L
Sbjct: 404 VTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPIL 463

Query: 485 QGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGT 544
           +GVK                                        LIAEAWDAGGLYQVG 
Sbjct: 464 RGVK----------------------------------------LIAEAWDAGGLYQVGM 523

Query: 545 FPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAH 604
           FPHWG+WSEWNGK+RD+VRQFIKGTDGFSGAFAECLCGSPNLYQ GGRKPW+SINFICAH
Sbjct: 524 FPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAH 583

Query: 605 DGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVC 664
           DGF+LADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F SISVK+LRKRQMRNFFV 
Sbjct: 584 DGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVS 643

Query: 665 LMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMTTFRY 724
           LMVSQ                           +NYFRWDK EE+ SDF RFC ++  FR 
Sbjct: 644 LMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRD 703

Query: 725 ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTV 784
           ECESLGLNDFPT +RLQWHG +P  P+W+E SRFVAF+LVDSVK+E+Y+AFN SHL   V
Sbjct: 704 ECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSVKKEIYVAFNTSHLATLV 763

Query: 785 MLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSIILLL 822
            LP RPGYRW+P VDT K SP+D +T DLPER+ A++QY HFLDAN+YPMLSYSSIILLL
Sbjct: 764 SLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIILLL 781

BLAST of CmUC06G122320 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 542.7 bits (1397), Expect = 5.0e-154
Identity = 321/777 (41.31%), Postives = 425/777 (54.70%), Query Frame = 0

Query: 57  RMNASVTRARNRGAEVGTKVTM----------AEEVEVSNLNR---FEVFDGTPTPFGAT 116
           +++  VTRAR    ++  + T+            E E S +     F+V  G  +P G +
Sbjct: 37  KLSRRVTRARIFSRKIKDRSTLKVTCRRAHERVVEEEASTMTETKLFKVSSGEVSPLGVS 96

Query: 117 TREGGINFAIYSANAVSATLCLI---NLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKG- 176
             + GINFA++S NA S TLCL    +  D  ++    E+ LDP  NKTGD WH+ ++  
Sbjct: 97  QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDL 156

Query: 177 DFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMV 236
               +LYGY+ DG    Q+G  FD S +LLDPYAK V     FG      +   + A   
Sbjct: 157 PLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFG------DSSQKFAQFY 216

Query: 237 PTVNEEIKNFDWEGDLPLQH-AQKDLIIYEMHVRGFTRHESSQSE--FPGTYLGLVEKLD 296
            T + E   FDW  D    +  +KDL+IYEM+VR FT  ESS  +    G+YLG +EK+ 
Sbjct: 217 GTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIP 276

Query: 297 YLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNC 356
           +L++LG+N +EL+P  EF+ELE     S   D+ +N WGYSTVNFF+PM RY+S G G+ 
Sbjct: 277 HLQDLGINAVELLPVFEFDELE-LQRRSNPRDHMVNTWGYSTVNFFAPMSRYAS-GEGD- 336

Query: 357 GQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGP-TISFRGVDNNVYYMLAPMGE 416
              A  EFK +++  H  GIEVI+DVV+NHT E ++  P T SFRG+DN VYYML P  +
Sbjct: 337 PIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQ 396

Query: 417 FYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYG 476
             N+SGCGNT NCNHP V   I+D LR+WVTE HVDGFRFDLAS++ R            
Sbjct: 397 LLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRA----------- 456

Query: 477 NPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEAL 536
                   T GSPL  PPLI  I+ D VL   K+                          
Sbjct: 457 --------TDGSPLSAPPLIRAIAKDSVLSRCKI-------------------------- 516

Query: 537 CSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGA 596
                         IAE WD GGLY VG FP+W  W+EWNG YRD VR+FIKG  G  G+
Sbjct: 517 --------------IAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGS 576

Query: 597 FAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNN 656
           FA  + GS +LYQ   RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE  NDG N N+
Sbjct: 577 FATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNH 636

Query: 657 SWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQ-------------------------- 716
           SWNCG EGE     +K LR RQM+NF + LM+SQ                          
Sbjct: 637 SWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDT 696

Query: 717 -LNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEA 776
            LN F+W +L+    +  RF S +  FR+    L   +F T   + WH     + +W  +
Sbjct: 697 SLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNS 740

Query: 777 -SRFVAFTLVDSV-KREVYIAFNASHLPVTVMLPE-RPGYRWDPLVDTGKISPFDFL 783
            S+F+AFTL D +  R++Y+AFNA    V  ++P+  PG +W  + DT   SP DF+
Sbjct: 757 ESKFLAFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFV 740

BLAST of CmUC06G122320 vs. TAIR 10
Match: AT1G03310.2 (debranching enzyme 1 )

HSP 1 Score: 285.0 bits (728), Expect = 1.9e-76
Identity = 215/730 (29.45%), Postives = 331/730 (45.34%), Query Frame = 0

Query: 95  GTPTPFGATT--REGGINFAIYSANAVSATLCLINLFDLQENRVTQEIRLDPLTNKTGDV 154
           G P P G ++   +   NF+ +S ++ +  LCL +  D   ++   E+ LDP  N+TGDV
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYD--DSTTDKPALELDLDPYVNRTGDV 289

Query: 155 WHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAV---ISRGEFGALGPND 214
           WH  +   +  + YGY+       +E    +   I+LDPYA  V   +S+   G+L  + 
Sbjct: 290 WHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSLSKS- 349

Query: 215 NCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQ--SEFPGT 274
                             +FDW  D+      + L++Y ++V+GFT+H SS+  S   GT
Sbjct: 350 -----------------PSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGT 409

Query: 275 YLGLVEKLDYLKELGVNCIELMPCHEFNELE--YFSYNSVLGDYKMNFWGYSTVNFFSPM 334
           + G+ EK+ +LK LG N + L P   F+E +  YF +                 +FFSPM
Sbjct: 410 FSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPF-----------------HFFSPM 469

Query: 335 IRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVDNN 394
             Y   G  N  + A+N  K+++++ H  GIEV+++VVF HTA+        + RG+D++
Sbjct: 470 DIY---GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDS 529

Query: 395 VYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGS 454
            YY      +  + S      NCN+P V+  +++ LRYWVTE HVDGF F  AS + RG 
Sbjct: 530 SYYYKGRANDLDSKS----YLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRG- 589

Query: 455 SLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQ 514
                               G  L  PPL++ I+ DP+L   K       L+ DC     
Sbjct: 590 ------------------VHGEQLSRPPLVEAIAFDPLLAETK-------LIADC----- 649

Query: 515 FFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQF 574
                        ++ +E+  KE+               FPHW  W+E N +Y   VR F
Sbjct: 650 -------------WDPLEMMPKEV--------------RFPHWKRWAELNTRYCRNVRNF 709

Query: 575 IKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGE 634
           ++G  G     A  +CGS +++   GR P  S N+I  + G SL D+V++ S   LA+  
Sbjct: 710 LRGR-GVLSDLATRICGSGDVFT-DGRGPAFSFNYISRNSGLSLVDIVSF-SGPELAS-- 769

Query: 635 DNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNF----FVCLMVSQLNY--------- 694
                     SWNCG+EG     +V + R +Q+RNF    ++ L V  LN          
Sbjct: 770 --------ELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTR 829

Query: 695 ----------FRWDKLEES-SSDFHRFCSLMTTFR-YECESLGLNDFPTDERLQWHGHSP 754
                     F W+ L  +  +   +F S MT+ R    +     DF   E + W+ +  
Sbjct: 830 GSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQ 838

Query: 755 GKPDWTE-ASRFVAFTLVDSVKRE---------------VYIAFNASHLPVTVMLPERP- 774
             P W + AS+F+A  +    + E               ++I FNAS  P +V+LP  P 
Sbjct: 890 TTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPD 838

BLAST of CmUC06G122320 vs. TAIR 10
Match: AT1G03310.1 (debranching enzyme 1 )

HSP 1 Score: 285.0 bits (728), Expect = 1.9e-76
Identity = 215/730 (29.45%), Postives = 331/730 (45.34%), Query Frame = 0

Query: 95  GTPTPFGATT--REGGINFAIYSANAVSATLCLINLFDLQENRVTQEIRLDPLTNKTGDV 154
           G P P G ++   +   NF+ +S ++ +  LCL +  D   ++   E+ LDP  N+TGDV
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYD--DSTTDKPALELDLDPYVNRTGDV 289

Query: 155 WHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAV---ISRGEFGALGPND 214
           WH  +   +  + YGY+       +E    +   I+LDPYA  V   +S+   G+L  + 
Sbjct: 290 WHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSLSKS- 349

Query: 215 NCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQ--SEFPGT 274
                             +FDW  D+      + L++Y ++V+GFT+H SS+  S   GT
Sbjct: 350 -----------------PSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGT 409

Query: 275 YLGLVEKLDYLKELGVNCIELMPCHEFNELE--YFSYNSVLGDYKMNFWGYSTVNFFSPM 334
           + G+ EK+ +LK LG N + L P   F+E +  YF +                 +FFSPM
Sbjct: 410 FSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPF-----------------HFFSPM 469

Query: 335 IRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVDNN 394
             Y   G  N  + A+N  K+++++ H  GIEV+++VVF HTA+        + RG+D++
Sbjct: 470 DIY---GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDS 529

Query: 395 VYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGS 454
            YY      +  + S      NCN+P V+  +++ LRYWVTE HVDGF F  AS + RG 
Sbjct: 530 SYYYKGRANDLDSKS----YLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRG- 589

Query: 455 SLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQ 514
                               G  L  PPL++ I+ DP+L   K       L+ DC     
Sbjct: 590 ------------------VHGEQLSRPPLVEAIAFDPLLAETK-------LIADC----- 649

Query: 515 FFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQF 574
                        ++ +E+  KE+               FPHW  W+E N +Y   VR F
Sbjct: 650 -------------WDPLEMMPKEV--------------RFPHWKRWAELNTRYCRNVRNF 709

Query: 575 IKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGE 634
           ++G  G     A  +CGS +++   GR P  S N+I  + G SL D+V++ S   LA+  
Sbjct: 710 LRGR-GVLSDLATRICGSGDVFT-DGRGPAFSFNYISRNSGLSLVDIVSF-SGPELAS-- 769

Query: 635 DNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNF----FVCLMVSQLNY--------- 694
                     SWNCG+EG     +V + R +Q+RNF    ++ L V  LN          
Sbjct: 770 --------ELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTR 829

Query: 695 ----------FRWDKLEES-SSDFHRFCSLMTTFR-YECESLGLNDFPTDERLQWHGHSP 754
                     F W+ L  +  +   +F S MT+ R    +     DF   E + W+ +  
Sbjct: 830 GSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQ 838

Query: 755 GKPDWTE-ASRFVAFTLVDSVKRE---------------VYIAFNASHLPVTVMLPERP- 774
             P W + AS+F+A  +    + E               ++I FNAS  P +V+LP  P 
Sbjct: 890 TTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPD 838

BLAST of CmUC06G122320 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 92.4 bits (228), Expect = 1.8e-18
Identity = 97/386 (25.13%), Postives = 154/386 (39.90%), Query Frame = 0

Query: 93  FDGTPTPFGATTREGGINFAIYSANAVSATLCLI-NLFDLQENRVTQEIRLDPLTNKTGD 152
           +DG   P GA     G++  +++  A + ++C+  N  D         + + PL    G 
Sbjct: 204 YDG---PLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLD------KSPMEICPLKEANG- 263

Query: 153 VWHVFLKGDFTEMLYGYK---FDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFGALGPN 212
           VW       +    Y YK   +       E CY +      DPYA+ + + G        
Sbjct: 264 VWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYAN------DPYARGLSADG-------- 323

Query: 213 DNCWPQMAGMVPTVNEEIKNFDWEG---DLPLQHAQKDLIIYEMHVRGFTRH-ESSQSEF 272
                +   +V   ++++K   W+      P   +  D+ IYE+HVR F+ + E+ + E 
Sbjct: 324 -----RKTFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPEN 383

Query: 273 PGTYLGLVEK-------LDYLKELGVNCIELMPCHEFNELEYFSYN-------------- 332
            G YL    K       L  L + G+  + L+P  +F +++    N              
Sbjct: 384 RGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRP 443

Query: 333 ----------SVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHK 392
                      +  D   N WGY+ V +  P   Y+S   G C    I EF+ +++  + 
Sbjct: 444 DSTEAQARITEIQNDDGYN-WGYNPVLWGVPKGSYASDPTGPC---RIIEFRKMVQALNC 503

Query: 393 RGIEVIMDVVFNHTAEGNENGPTISFRGVDNNV--YYMLAPMGEFYNYSGCGNTFNCNHP 438
            G+ V++DVV+NH    + +GP      +D  V  YY+      F   S C N     H 
Sbjct: 504 TGLNVVLDVVYNHL---HASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHY 553