Homology
BLAST of CmUC06G122320 vs. NCBI nr
Match:
XP_038879251.1 (isoamylase 1, chloroplastic [Benincasa hispida])
HSP 1 Score: 1550.0 bits (4012), Expect = 0.0e+00
Identity = 753/852 (88.38%), Postives = 765/852 (89.79%), Query Frame = 0
Query: 1 MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
MEL+TRSASLSSSASFLC SKL F ECRRRNSKQNVVR RRRSDIRFDLDVN HSRMNA
Sbjct: 1 MELVTRSASLSSSASFLCASKLNFPECRRRNSKQNVVRDRRRSDIRFDLDVNWFHSRMNA 60
Query: 61 SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
SVTRA NRGA+VGT + MAEEVEVS NRFEVFDGTPTPFGATTR+GG+NFAIYSANAVS
Sbjct: 61 SVTRAWNRGAKVGTSIAMAEEVEVSISNRFEVFDGTPTPFGATTRDGGVNFAIYSANAVS 120
Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
ATLCLINL DL EN VTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKF+GKFSPQEGC
Sbjct: 121 ATLCLINLSDLHENSVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFEGKFSPQEGC 180
Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT+NEEIKNFDWEGDLPLQHA
Sbjct: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTINEEIKNFDWEGDLPLQHA 240
Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF
Sbjct: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
SYNSVLGDYKMNFWGYSTVN+FSPMIRYSSA IGNCGQDAINEFKLLIREAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKMNFWGYSTVNYFSPMIRYSSASIGNCGQDAINEFKLLIREAHKRGIEVIM 360
Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVDC 420
Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNV+GNPIEGDVLTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVFGNPIEGDVLTTGSPLGNPPLIDMISN 480
Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
DPVLQGVK LIAEAWDAGGLY
Sbjct: 481 DPVLQGVK----------------------------------------LIAEAWDAGGLY 540
Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
QVGTFPHWGVWSEWNG+YRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
ICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
FFVCLMVSQ LNYFRWDK+EESSSDFHRFCSLMT
Sbjct: 661 FFVCLMVSQGVPLILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESSSDFHRFCSLMT 720
Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL
Sbjct: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 826
PVTV LPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQY HFLDANLYPMLSYSSI
Sbjct: 781 PVTVTLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYVHFLDANLYPMLSYSSI 812
BLAST of CmUC06G122320 vs. NCBI nr
Match:
XP_008453530.1 (PREDICTED: isoamylase 1, chloroplastic [Cucumis melo] >KAA0058172.1 isoamylase 1 [Cucumis melo var. makuwa])
HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 734/853 (86.05%), Postives = 755/853 (88.51%), Query Frame = 0
Query: 1 MELITRS-ASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMN 60
MEL+TRS ASL SSASFLC S L F ECRRRN KQNVVRGRRR+DI FDLD N IHS +N
Sbjct: 1 MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60
Query: 61 ASVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAV 120
RARNRGAEVGT V +AEE E+SNLNRFEVFDGTPTPFGAT R+ GINFAIYSANAV
Sbjct: 61 ----RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAV 120
Query: 121 SATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
SATLCL++L DLQEN VTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG
Sbjct: 121 SATLCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
Query: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQH 240
CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT NE+IKNFDWEGDLPLQH
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQH 240
Query: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
AQKDLIIYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
Query: 301 FSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
FSYNSVLGDYKMNFWGYSTVN+FSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
Query: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVD 420
MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420
Query: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 480
Query: 481 NDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGL 540
NDPVLQGVK LIAEAWDAGGL
Sbjct: 481 NDPVLQGVK----------------------------------------LIAEAWDAGGL 540
Query: 541 YQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
YQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKP NSIN
Sbjct: 541 YQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPCNSIN 600
Query: 601 FICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
F+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR
Sbjct: 601 FVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
Query: 661 NFFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLM 720
NFFVCLMVSQ LNYFRWDK+EES+SDFHRFCSLM
Sbjct: 661 NFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSLM 720
Query: 721 TTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
TTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH
Sbjct: 721 TTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
Query: 781 LPVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSS 826
LPVTV LPERPGYRWDPLVDTGK SPFDFLT+D+PER++AIQQYAHFLDANLYPMLSYSS
Sbjct: 781 LPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQYAHFLDANLYPMLSYSS 809
BLAST of CmUC06G122320 vs. NCBI nr
Match:
XP_004149676.1 (isoamylase 1, chloroplastic [Cucumis sativus] >KAE8653176.1 hypothetical protein Csa_019536 [Cucumis sativus])
HSP 1 Score: 1503.4 bits (3891), Expect = 0.0e+00
Identity = 735/854 (86.07%), Postives = 756/854 (88.52%), Query Frame = 0
Query: 1 MELITRS-ASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMN 60
MEL+T S ASL SSASFLC S L F ECRRRNSKQNVVRGRRRSDI FDLD N IHS +N
Sbjct: 1 MELVTHSAASLFSSASFLCVSNLSFPECRRRNSKQNVVRGRRRSDICFDLDANWIHSSLN 60
Query: 61 ASVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAV 120
RARNRGAEV T V +AEEVEVSNLNRFEVFDGTPTPFGAT RE GINFAIYSANAV
Sbjct: 61 ----RARNRGAEVRTSVAVAEEVEVSNLNRFEVFDGTPTPFGATAREDGINFAIYSANAV 120
Query: 121 SATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
SATLCLI+L DLQENRVTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG
Sbjct: 121 SATLCLIHLSDLQENRVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
Query: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQH 240
CYFD SRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT NE+IK+FDWEGDLP+QH
Sbjct: 181 CYFDSSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKDFDWEGDLPVQH 240
Query: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELG+NCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGINCIELMPCHEFNELEY 300
Query: 301 FSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
FSYNSVLGDYKMNFWGYSTVN+FSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
Query: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVD 420
MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420
Query: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYG+PIEGD+LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGSPIEGDILTTGSPLGNPPLIDMIS 480
Query: 481 NDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGL 540
NDPVLQGVK LIAEAWDAGGL
Sbjct: 481 NDPVLQGVK----------------------------------------LIAEAWDAGGL 540
Query: 541 YQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
YQVGTFPHWGVWSEWNGKYRD++RQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN
Sbjct: 541 YQVGTFPHWGVWSEWNGKYRDVIRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
Query: 601 FICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
FICAHDGF+LADLVTYNSK NLANGE+NNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR
Sbjct: 601 FICAHDGFTLADLVTYNSKQNLANGEENNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
Query: 661 NFFVCLMVSQ---------------------------LNYFRWDKLEESSS-DFHRFCSL 720
NFFVCLMVSQ LNYFRWDK+EES+S DFHRFCSL
Sbjct: 661 NFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSFDFHRFCSL 720
Query: 721 MTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNAS 780
MTTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNAS
Sbjct: 721 MTTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNAS 780
Query: 781 HLPVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYS 826
HLPVTV LPERPGYRWDPLVDTGK SPFDFLT+D+P+RDVAIQQYAHFLDAN YPMLSYS
Sbjct: 781 HLPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPKRDVAIQQYAHFLDANYYPMLSYS 810
BLAST of CmUC06G122320 vs. NCBI nr
Match:
XP_022134724.1 (isoamylase 1, chloroplastic [Momordica charantia])
HSP 1 Score: 1448.0 bits (3747), Expect = 0.0e+00
Identity = 702/851 (82.49%), Postives = 737/851 (86.60%), Query Frame = 0
Query: 1 MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
MEL+TRS SL SA FLC K RF ECRRR+SK N VR RRRSDI DLD N I+SRM
Sbjct: 1 MELVTRSTSLCWSACFLCAPKSRFPECRRRSSKNNAVRSRRRSDIHMDLDANWINSRMKM 60
Query: 61 SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
S+TRA NRGAEVGT V MAEEVEVSNL+RFEVF+G P P GAT R+GG+NFA+YS NAVS
Sbjct: 61 SLTRASNRGAEVGTSVAMAEEVEVSNLHRFEVFEGDPAPLGATARDGGMNFAVYSGNAVS 120
Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
ATLCL+NLFDLQENRVT+E+ LDPLTNKTGDVWHVFLKG FTEMLYGYKFDG+FSPQ+GC
Sbjct: 121 ATLCLMNLFDLQENRVTKEVTLDPLTNKTGDVWHVFLKGGFTEMLYGYKFDGEFSPQDGC 180
Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
YFDPSRILLDPYAKAVISRGEFGA GP++NCWPQMAGMVPTV +E FDWEGDLPLQ+
Sbjct: 181 YFDPSRILLDPYAKAVISRGEFGAPGPDNNCWPQMAGMVPTVIDE---FDWEGDLPLQYP 240
Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
QKDLIIYEMHVRGFTRHESS SEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF
Sbjct: 241 QKDLIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
SYNSVLGDYKMNFWGYSTV FFSPMIRYSSAGI CGQDAINEFKLLIREAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLIREAHKRGIEVIM 360
Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
DVVFNHTAEGNENGP SFRGVDN+VYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420
Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
DPVLQGVK LIAEAWDAGGLY
Sbjct: 481 DPVLQGVK----------------------------------------LIAEAWDAGGLY 540
Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
QVGTFPHWGVWSEWNG+YRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
ICAHDGF+LADLVTYN+KHNLANGEDNNDGE+HNNSWNCGQEGEFVS+SVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFVSMSVKKLRKRQMRN 660
Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
FFVCLMVSQ +NYFRWDKLEESSSDFHRFCSL+T
Sbjct: 661 FFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWDKLEESSSDFHRFCSLVT 720
Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
TFR+ECESLGLNDFPT ERLQWHG+SPGKPDW+EASRFVAFTLVDSVK E+YIAFNA+HL
Sbjct: 721 TFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFVAFTLVDSVKGEIYIAFNANHL 780
Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 825
PVTV LPERPGYRW+PLVDTGKISPFDFL+SDLPERDVAI+QYAHFLD NLYPML YSSI
Sbjct: 781 PVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQYAHFLDNNLYPMLCYSSI 808
BLAST of CmUC06G122320 vs. NCBI nr
Match:
XP_022932124.1 (isoamylase 1, chloroplastic [Cucurbita moschata])
HSP 1 Score: 1443.7 bits (3736), Expect = 0.0e+00
Identity = 704/852 (82.63%), Postives = 735/852 (86.27%), Query Frame = 0
Query: 1 MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
MEL+TRSASLSSS SFL S F E RRR+SK NV+ RRRSDI DL V+ IHSR+N
Sbjct: 1 MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
Query: 61 SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
S TRA +RGAEV T VT+AEEVE+SN NRFEVF+GTPTPFGAT R+GGINFAIYS NAVS
Sbjct: 61 SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
ATLCLINLFDLQ+ RV QEI LDPL N+TGDVWHVFLKGDFT+MLYGYKF GKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
YFDPSRILLDPYAKAVISR EFGALGP+DNCWPQMAGMVPTV++E FDWEGDLPLQH
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQHP 240
Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
QKDLIIYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY+
Sbjct: 241 QKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYY 300
Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
SYNSVLGDYK+NFWGYSTV F SPMIRYSSAGI NCGQDAINEFKLLI+EAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIM 360
Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
DVVFNHTAEGNENGP SFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420
Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
LRYWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISN 480
Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
DP+LQGVK LIAEAWDAGGLY
Sbjct: 481 DPILQGVK----------------------------------------LIAEAWDAGGLY 540
Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
QVGTFPHW VWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
ICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
FFVCLMVSQ +NYFRWDK+EESSSDFHRFC LMT
Sbjct: 661 FFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEESSSDFHRFCRLMT 720
Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
TFRYECESLGLNDFPT +RLQWHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHL
Sbjct: 721 TFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHL 780
Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 826
PVTV LPERPGY+W+PLVDTGKISPFDFLTSDL ERDVAIQQYAHFLDANLYPMLSYSSI
Sbjct: 781 PVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQYAHFLDANLYPMLSYSSI 809
BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match:
O04196 (Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1)
HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 534/784 (68.11%), Postives = 623/784 (79.46%), Query Frame = 0
Query: 65 ARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVSATLC 124
A++R + + + E+ S +RF + DG P+PFG T R+ G+NF++YS N+VSAT+C
Sbjct: 44 AKDRRSNEAENIAVVEKPLKS--DRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATIC 103
Query: 125 LINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDP 184
LI+L DL++N+VT+EI+LDP N+TG VWHVFL+GDF +MLYGY+FDGKFSP+EG Y+D
Sbjct: 104 LISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDS 163
Query: 185 SRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDL 244
S ILLDPYAKA+ISR EFG LGP+DNCWPQMA MVPT EE FDWEGD+ L+ QKDL
Sbjct: 164 SNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEE---FDWEGDMHLKLPQKDL 223
Query: 245 IIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNS 304
+IYEMHVRGFTRHESS+ EFPGTY G+ EKLD+LKELG+NCIELMPCHEFNELEY+SYN+
Sbjct: 224 VIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNT 283
Query: 305 VLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVF 364
+LGD+++NFWGYST+ FFSPMIRY+SA N AINEFK+L++EAHKRGIEVIMDVV
Sbjct: 284 ILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVL 343
Query: 365 NHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYW 424
NHTAEGNE GP SFRGVDN+VYYMLAP GEFYNYSGCGNTFNCNHP VR FI+DCLRYW
Sbjct: 344 NHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYW 403
Query: 425 VTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVL 484
VTEMHVDGFRFDL SIM+R SSLWDA NVYG +EGD+LTTG+P+ PP+IDMISNDP+L
Sbjct: 404 VTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPIL 463
Query: 485 QGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGT 544
+GVK LIAEAWDAGGLYQVG
Sbjct: 464 RGVK----------------------------------------LIAEAWDAGGLYQVGM 523
Query: 545 FPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAH 604
FPHWG+WSEWNGK+RD+VRQFIKGTDGFSGAFAECLCGSPNLYQ GGRKPW+SINFICAH
Sbjct: 524 FPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAH 583
Query: 605 DGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVC 664
DGF+LADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F SISVK+LRKRQMRNFFV
Sbjct: 584 DGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVS 643
Query: 665 LMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMTTFRY 724
LMVSQ +NYFRWDK EE+ SDF RFC ++ FR
Sbjct: 644 LMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRD 703
Query: 725 ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTV 784
ECESLGLNDFPT +RLQWHG +P P+W+E SRFVAF+LVDSVK+E+Y+AFN SHL V
Sbjct: 704 ECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSVKKEIYVAFNTSHLATLV 763
Query: 785 MLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSIILLL 822
LP RPGYRW+P VDT K SP+D +T DLPER+ A++QY HFLDAN+YPMLSYSSIILLL
Sbjct: 764 SLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIILLL 781
BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match:
D0TZF0 (Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=1 SV=1)
HSP 1 Score: 1091.6 bits (2822), Expect = 0.0e+00
Identity = 527/823 (64.03%), Postives = 604/823 (73.39%), Query Frame = 0
Query: 28 RRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNASVTRARNRGAEVGTK----VTMAEEVE 87
RRRN+ + RR +R + S + V G E + V M E
Sbjct: 35 RRRNAAFSAGNAGRRVGLR-----RSVASAVEVGVGEDEEEGVEEEEEEVEAVVMPERYA 94
Query: 88 VSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVSATLCLINLFDLQENRVTQEIRLD 147
+ R V G P P GAT +GG+NFA+YSA A +A+LCL DL+ + VT+E+ LD
Sbjct: 95 LGGACR--VLAGMPAPLGATALDGGVNFAVYSAGASAASLCLFTPDDLEADEVTEEVPLD 154
Query: 148 PLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFG 207
PL N+TG+VWHVF++G+ MLYGY+FDG F+P G YFD S +++DPYAKAVISRGE+G
Sbjct: 155 PLFNRTGNVWHVFIEGELHNMLYGYRFDGMFAPHCGQYFDVSNVVVDPYAKAVISRGEYG 214
Query: 208 ALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQSE 267
GP +CWPQMAGM+P FDW+GDLPL++ QKDL+IYEMH+RGFT+H SS E
Sbjct: 215 VPGPGGDCWPQMAGMIPL---PYSTFDWQGDLPLRYPQKDLVIYEMHLRGFTKHSSSNVE 274
Query: 268 FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNFFS 327
PGTY+G + KLDYLKELGVNC+ELMPCHEFNELEYFS +S KMNFWGYST+NFFS
Sbjct: 275 HPGTYIGAISKLDYLKELGVNCVELMPCHEFNELEYFSCSS-----KMNFWGYSTINFFS 334
Query: 328 PMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVD 387
PMIRYSS GI NCG+DAINEFK +REAHKRGIEVIMDVVFNHTAEGNE GP +SFRG+D
Sbjct: 335 PMIRYSSGGIRNCGRDAINEFKTFVREAHKRGIEVIMDVVFNHTAEGNEKGPILSFRGID 394
Query: 388 NNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTR 447
N+ YYMLAP GEFYNYSGCGNTFNCNHP VR FIVDCLRYWVTEMHVDGFRFDLASIMTR
Sbjct: 395 NSTYYMLAPKGEFYNYSGCGNTFNCNHPVVREFIVDCLRYWVTEMHVDGFRFDLASIMTR 454
Query: 448 GSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLH 507
G SLWD VNVYG+P+EGD+ TTG+PL PPLIDMISNDP+L VK
Sbjct: 455 GCSLWDPVNVYGSPVEGDMTTTGTPLATPPLIDMISNDPILGDVK--------------- 514
Query: 508 GQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVR 567
LIAEAWDAGGLYQVG FPHW +WSEWNGKYRDIVR
Sbjct: 515 -------------------------LIAEAWDAGGLYQVGQFPHWKIWSEWNGKYRDIVR 574
Query: 568 QFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLAN 627
QFIKGTDGF+G FAECLCGSP+LYQ GGRKPW+SINF+CAHDGF+LADLVTYN K+N +N
Sbjct: 575 QFIKGTDGFAGGFAECLCGSPHLYQAGGRKPWHSINFVCAHDGFTLADLVTYNKKYNSSN 634
Query: 628 GEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQ-------------- 687
GEDN DGENHN SWNCG+EGEF +SVK+LRKRQMRNFFV LMVSQ
Sbjct: 635 GEDNRDGENHNLSWNCGEEGEFAGLSVKRLRKRQMRNFFVSLMVSQGVPMFYMGDEYGHT 694
Query: 688 -------------LNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWH 747
+NYFRWDK EE SSD RFCSLMT FR +CESLGL DFPT +RL WH
Sbjct: 695 KGGNNNTYCHDHYVNYFRWDKKEE-SSDLQRFCSLMTKFRKQCESLGLADFPTAQRLHWH 754
Query: 748 GHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTVMLPERPGYRWDPLVDTGKI 807
GH PGKPDW+E SRFVAF+ D K E+Y+AFNASHLP V LPERPGYRW+PLVDTGK
Sbjct: 755 GHQPGKPDWSETSRFVAFSTKDETKGEIYVAFNASHLPAVVGLPERPGYRWEPLVDTGKP 801
Query: 808 SPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSIILLLSP 820
+P+DFLT DLP+R A+ ++HFL++NLYPMLSYSSIIL L P
Sbjct: 815 APYDFLTDDLPDRAHAVHLFSHFLNSNLYPMLSYSSIILELQP 801
BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match:
Q9M0S5 (Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2)
HSP 1 Score: 542.7 bits (1397), Expect = 7.0e-153
Identity = 321/777 (41.31%), Postives = 425/777 (54.70%), Query Frame = 0
Query: 57 RMNASVTRARNRGAEVGTKVTM----------AEEVEVSNLNR---FEVFDGTPTPFGAT 116
+++ VTRAR ++ + T+ E E S + F+V G +P G +
Sbjct: 37 KLSRRVTRARIFSRKIKDRSTLKVTCRRAHERVVEEEASTMTETKLFKVSSGEVSPLGVS 96
Query: 117 TREGGINFAIYSANAVSATLCLI---NLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKG- 176
+ GINFA++S NA S TLCL + D ++ E+ LDP NKTGD WH+ ++
Sbjct: 97 QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDL 156
Query: 177 DFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMV 236
+LYGY+ DG Q+G FD S +LLDPYAK V FG + + A
Sbjct: 157 PLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFG------DSSQKFAQFY 216
Query: 237 PTVNEEIKNFDWEGDLPLQH-AQKDLIIYEMHVRGFTRHESSQSE--FPGTYLGLVEKLD 296
T + E FDW D + +KDL+IYEM+VR FT ESS + G+YLG +EK+
Sbjct: 217 GTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIP 276
Query: 297 YLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNC 356
+L++LG+N +EL+P EF+ELE S D+ +N WGYSTVNFF+PM RY+S G G+
Sbjct: 277 HLQDLGINAVELLPVFEFDELE-LQRRSNPRDHMVNTWGYSTVNFFAPMSRYAS-GEGD- 336
Query: 357 GQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGP-TISFRGVDNNVYYMLAPMGE 416
A EFK +++ H GIEVI+DVV+NHT E ++ P T SFRG+DN VYYML P +
Sbjct: 337 PIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQ 396
Query: 417 FYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYG 476
N+SGCGNT NCNHP V I+D LR+WVTE HVDGFRFDLAS++ R
Sbjct: 397 LLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRA----------- 456
Query: 477 NPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEAL 536
T GSPL PPLI I+ D VL K+
Sbjct: 457 --------TDGSPLSAPPLIRAIAKDSVLSRCKI-------------------------- 516
Query: 537 CSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGA 596
IAE WD GGLY VG FP+W W+EWNG YRD VR+FIKG G G+
Sbjct: 517 --------------IAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGS 576
Query: 597 FAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNN 656
FA + GS +LYQ RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE NDG N N+
Sbjct: 577 FATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNH 636
Query: 657 SWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQ-------------------------- 716
SWNCG EGE +K LR RQM+NF + LM+SQ
Sbjct: 637 SWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDT 696
Query: 717 -LNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEA 776
LN F+W +L+ + RF S + FR+ L +F T + WH + +W +
Sbjct: 697 SLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNS 740
Query: 777 -SRFVAFTLVDSV-KREVYIAFNASHLPVTVMLPE-RPGYRWDPLVDTGKISPFDFL 783
S+F+AFTL D + R++Y+AFNA V ++P+ PG +W + DT SP DF+
Sbjct: 757 ESKFLAFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFV 740
BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match:
B9G434 (Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=2 SV=1)
HSP 1 Score: 521.9 bits (1343), Expect = 1.3e-146
Identity = 319/798 (39.97%), Postives = 424/798 (53.13%), Query Frame = 0
Query: 63 TRARNRGAEVGTKVTMAEEVEVSNLNR-FEVFDGTPTPFGATTREGGINFAIYSANAVSA 122
T AR + G +T +S+ F+ G P G + EGG+NFA++S +A S
Sbjct: 70 TTARAQNGNAGRSMTEERGCTMSDTEMPFKYSSGKAFPLGVSQVEGGLNFALFSQHASSV 129
Query: 123 TLCLI---NLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKG-DFTEMLYGYKFDGKFSPQ 182
LCL + ++ E LD NKTGD+WHV ++G + +LYGY+ G
Sbjct: 130 ILCLKLPGRGTEDEKGADVVEFVLDQQKNKTGDIWHVIVEGLPASGVLYGYRVGGPQGWD 189
Query: 183 EGCYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPL 242
+G FD S +LLDPYAK V R FG + Q G T + + FDW D L
Sbjct: 190 QGHRFDSSTVLLDPYAKLVSGRKYFGVAEEKSS---QHFG---TYDFDSSPFDWGDDYRL 249
Query: 243 QH-AQKDLIIYEMHVRGFTRHESS--QSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEF 302
+ + DL+IYEM+VR FT ESS S G+YLGL++K+ +L ELGVN +EL+P E+
Sbjct: 250 PNLPEADLVIYEMNVRAFTADESSGLDSTSRGSYLGLIDKIPHLLELGVNAVELLPVFEY 309
Query: 303 NELEYFSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKR 362
+ELE+ Y + D+ +N WGYST+NFF+PM RY+SAG G A E K +++E HK
Sbjct: 310 DELEFKRYPNP-RDHMVNTWGYSTINFFAPMSRYASAGGGPVA--ASKELKQMVKELHKA 369
Query: 363 GIEVIMDVVFNHTAEGNENGPTI-SFRGVDNNVYYM--LAPMGEFYNYSGCGNTFNCNHP 422
GIEVI+DVV+NHT E ++ P + SFRG+DN VYYM L E N+SGCGNT NCNHP
Sbjct: 370 GIEVILDVVYNHTNEADDAHPYMTSFRGIDNKVYYMLDLNKNAELLNFSGCGNTLNCNHP 429
Query: 423 AVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGN 482
V+ I+D LR+WV E H+DGFRFDLAS++ RG G PL
Sbjct: 430 VVKELILDSLRHWVEEYHIDGFRFDLASVLCRGPD-------------------GCPLDA 489
Query: 483 PPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIA 542
PPLI I+ D VL K+ IA
Sbjct: 490 PPLIKEIAKDAVLSRCKI----------------------------------------IA 549
Query: 543 EAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGG 602
E WD GGLY VG FP+W W+EWNGKYRD +R+FIKG G G FA + GS +LYQ
Sbjct: 550 EPWDCGGLYLVGRFPNWDRWAEWNGKYRDDLRRFIKGDPGMKGVFATRVSGSADLYQVNE 609
Query: 603 RKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVK 662
RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE DG N N SWNCG EGE ++V
Sbjct: 610 RKPYHGVNFVIAHDGFTLCDLVSYNLKHNDANGEGGCDGCNDNFSWNCGVEGETNDLNVL 669
Query: 663 KLRKRQMRNFFVCLMVSQ---------------------------LNYFRWDKLEESSSD 722
LR RQM+NF V LM+SQ +N F+W++LE+
Sbjct: 670 SLRSRQMKNFHVALMISQGTPMMLMGDEYGHTRYGNNNSYGHDTCINNFQWEQLEQRRDG 729
Query: 723 FHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDW-TEASRFVAFTLVD-SVKR 782
RF S M FR+ L + F + WH + W + S+F+AFT+ D +
Sbjct: 730 HFRFFSEMIKFRHSNPILRRDRFLNKNDVTWH-----EDCWENQESKFLAFTVHDHNSGG 782
Query: 783 EVYIAFNASHLPVTVMLPERPGYR-WDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLD 820
++Y+AFNA V ++P P ++ W+ +VDT SP D + +P
Sbjct: 790 DIYLAFNAHDYFVDAVIPPPPHHKCWNRVVDTNLESPNDIVPEGVP------------FT 782
BLAST of CmUC06G122320 vs. ExPASy Swiss-Prot
Match:
P0A4Y5 (Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935 / AF2122/97) OX=233413 GN=glgX PE=3 SV=1)
HSP 1 Score: 404.1 bits (1037), Expect = 3.9e-111
Identity = 244/596 (40.94%), Postives = 314/596 (52.68%), Query Frame = 0
Query: 92 VFDGTPTPFGATTREGGINFAIYSANAVSATLCLINLFDLQENRVTQEIRLDPLTNKTGD 151
V+ G P GAT G NF+++S A LCLI+ E+ V I PL G
Sbjct: 19 VWPGNAYPLGATYDGAGTNFSLFSEIAEKVELCLID-----EDGVESRI---PLDEVDGY 78
Query: 152 VWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFG-------- 211
VWH +L YG++ G F P G DPS++LLDPY K+ FG
Sbjct: 79 VWHAYLPNITPGQRYGFRVHGPFDPAAGHRCDPSKLLLDPYGKSFHGDFTFGQALYSYDV 138
Query: 212 -ALGPNDNCWPQMAGMVPTVNEEIKN--FDWEGDLPLQHAQKDLIIYEMHVRGFTR-HES 271
A+ P D+ P + + T+ + N FDW D + + +IYE HV+G T+ H S
Sbjct: 139 NAVDP-DSTPPMVDSLGHTMTSVVINPFFDWAYDRSPRTPYHETVIYEAHVKGMTQTHPS 198
Query: 272 SQSEFPGTYLGLVEK--LDYLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKM-NFWGY 331
E GTY GL +D+L EL V +ELMP H+ F ++S L D + N+WGY
Sbjct: 199 IPPELRGTYAGLAHPVIIDHLNELNVTAVELMPVHQ------FLHDSRLLDLGLRNYWGY 258
Query: 332 STVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPT 391
+T FF+P +Y+S A+ EFK ++R H+ GIEVI+DVV+NHTAEGN GPT
Sbjct: 259 NTFGFFAPHHQYAST---RQAGSAVAEFKTMVRSLHEAGIEVILDVVYNHTAEGNHLGPT 318
Query: 392 ISFRGVDNNVYYMLAPMG-EFY-NYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFR 451
I+FRG+DN YY L FY +++G GN+ N HP I+D LRYWV EMHVDGFR
Sbjct: 319 INFRGIDNTAYYRLMDHDLRFYKDFTGTGNSLNARHPHTLQLIMDSLRYWVIEMHVDGFR 378
Query: 452 FDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNS 511
FDLAS + R D ++ + D++ DPV+ VK
Sbjct: 379 FDLASTLARELHDVDRLSAF--------------------FDLVQQDPVVSQVK------ 438
Query: 512 SLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAG-GLYQVGTFPHWGVWSE 571
LIAE WD G G YQVG FP G+W+E
Sbjct: 439 ----------------------------------LIAEPWDVGEGGYQVGNFP--GLWTE 498
Query: 572 WNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLV 631
WNGKYRD VR + +G G FA L GS +LY+ GR+P SINF+ AHDGF+L DLV
Sbjct: 499 WNGKYRDTVRDYWRGEPATLGEFASRLTGSSDLYEATGRRPSASINFVTAHDGFTLNDLV 534
Query: 632 TYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQ 670
+YN KHN ANGE+N DGE++N SWNCG EG + LR RQMRN + LMVSQ
Sbjct: 559 SYNDKHNEANGENNRDGESYNRSWNCGVEGPTDDPDILALRARQMRNMWATLMVSQ 534
BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match:
A0A5A7UQM5 (Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 PE=4 SV=1)
HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 734/853 (86.05%), Postives = 755/853 (88.51%), Query Frame = 0
Query: 1 MELITRS-ASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMN 60
MEL+TRS ASL SSASFLC S L F ECRRRN KQNVVRGRRR+DI FDLD N IHS +N
Sbjct: 1 MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60
Query: 61 ASVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAV 120
RARNRGAEVGT V +AEE E+SNLNRFEVFDGTPTPFGAT R+ GINFAIYSANAV
Sbjct: 61 ----RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAV 120
Query: 121 SATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
SATLCL++L DLQEN VTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG
Sbjct: 121 SATLCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
Query: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQH 240
CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT NE+IKNFDWEGDLPLQH
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQH 240
Query: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
AQKDLIIYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
Query: 301 FSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
FSYNSVLGDYKMNFWGYSTVN+FSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
Query: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVD 420
MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420
Query: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 480
Query: 481 NDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGL 540
NDPVLQGVK LIAEAWDAGGL
Sbjct: 481 NDPVLQGVK----------------------------------------LIAEAWDAGGL 540
Query: 541 YQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
YQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKP NSIN
Sbjct: 541 YQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPCNSIN 600
Query: 601 FICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
F+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR
Sbjct: 601 FVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
Query: 661 NFFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLM 720
NFFVCLMVSQ LNYFRWDK+EES+SDFHRFCSLM
Sbjct: 661 NFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSLM 720
Query: 721 TTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
TTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH
Sbjct: 721 TTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
Query: 781 LPVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSS 826
LPVTV LPERPGYRWDPLVDTGK SPFDFLT+D+PER++AIQQYAHFLDANLYPMLSYSS
Sbjct: 781 LPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQYAHFLDANLYPMLSYSS 809
BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match:
A0A1S3BVW8 (isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1)
HSP 1 Score: 1506.5 bits (3899), Expect = 0.0e+00
Identity = 734/853 (86.05%), Postives = 755/853 (88.51%), Query Frame = 0
Query: 1 MELITRS-ASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMN 60
MEL+TRS ASL SSASFLC S L F ECRRRN KQNVVRGRRR+DI FDLD N IHS +N
Sbjct: 1 MELVTRSAASLFSSASFLCVSNLSFPECRRRNLKQNVVRGRRRNDICFDLDANWIHSSLN 60
Query: 61 ASVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAV 120
RARNRGAEVGT V +AEE E+SNLNRFEVFDGTPTPFGAT R+ GINFAIYSANAV
Sbjct: 61 ----RARNRGAEVGTSVAVAEEFEISNLNRFEVFDGTPTPFGATARDDGINFAIYSANAV 120
Query: 121 SATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
SATLCL++L DLQEN VTQEI LDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG
Sbjct: 121 SATLCLMHLSDLQENIVTQEITLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEG 180
Query: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQH 240
CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPT NE+IKNFDWEGDLPLQH
Sbjct: 181 CYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTANEKIKNFDWEGDLPLQH 240
Query: 241 AQKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
AQKDLIIYEMHVRGFTRHESSQS FPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY
Sbjct: 241 AQKDLIIYEMHVRGFTRHESSQSAFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEY 300
Query: 301 FSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
FSYNSVLGDYKMNFWGYSTVN+FSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI
Sbjct: 301 FSYNSVLGDYKMNFWGYSTVNYFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVI 360
Query: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVD 420
MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVD
Sbjct: 361 MDVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRTFIVD 420
Query: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMIS 480
CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD+LTTGSPLGNPPLIDMIS
Sbjct: 421 CLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDILTTGSPLGNPPLIDMIS 480
Query: 481 NDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGL 540
NDPVLQGVK LIAEAWDAGGL
Sbjct: 481 NDPVLQGVK----------------------------------------LIAEAWDAGGL 540
Query: 541 YQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSIN 600
YQVGTFPHWGVWSEWNGKYRD+VRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKP NSIN
Sbjct: 541 YQVGTFPHWGVWSEWNGKYRDVVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPCNSIN 600
Query: 601 FICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
F+CAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR
Sbjct: 601 FVCAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMR 660
Query: 661 NFFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLM 720
NFFVCLMVSQ LNYFRWDK+EES+SDFHRFCSLM
Sbjct: 661 NFFVCLMVSQGVPMILMGDEYGHTKGGNNNTYCHDNYLNYFRWDKMEESTSDFHRFCSLM 720
Query: 721 TTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
TTFR+ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH
Sbjct: 721 TTFRHECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASH 780
Query: 781 LPVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSS 826
LPVTV LPERPGYRWDPLVDTGK SPFDFLT+D+PER++AIQQYAHFLDANLYPMLSYSS
Sbjct: 781 LPVTVTLPERPGYRWDPLVDTGKNSPFDFLTNDIPERNLAIQQYAHFLDANLYPMLSYSS 809
BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match:
A0A6J1BZK5 (isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 SV=1)
HSP 1 Score: 1448.0 bits (3747), Expect = 0.0e+00
Identity = 702/851 (82.49%), Postives = 737/851 (86.60%), Query Frame = 0
Query: 1 MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
MEL+TRS SL SA FLC K RF ECRRR+SK N VR RRRSDI DLD N I+SRM
Sbjct: 1 MELVTRSTSLCWSACFLCAPKSRFPECRRRSSKNNAVRSRRRSDIHMDLDANWINSRMKM 60
Query: 61 SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
S+TRA NRGAEVGT V MAEEVEVSNL+RFEVF+G P P GAT R+GG+NFA+YS NAVS
Sbjct: 61 SLTRASNRGAEVGTSVAMAEEVEVSNLHRFEVFEGDPAPLGATARDGGMNFAVYSGNAVS 120
Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
ATLCL+NLFDLQENRVT+E+ LDPLTNKTGDVWHVFLKG FTEMLYGYKFDG+FSPQ+GC
Sbjct: 121 ATLCLMNLFDLQENRVTKEVTLDPLTNKTGDVWHVFLKGGFTEMLYGYKFDGEFSPQDGC 180
Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
YFDPSRILLDPYAKAVISRGEFGA GP++NCWPQMAGMVPTV +E FDWEGDLPLQ+
Sbjct: 181 YFDPSRILLDPYAKAVISRGEFGAPGPDNNCWPQMAGMVPTVIDE---FDWEGDLPLQYP 240
Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
QKDLIIYEMHVRGFTRHESS SEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF
Sbjct: 241 QKDLIIYEMHVRGFTRHESSHSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
SYNSVLGDYKMNFWGYSTV FFSPMIRYSSAGI CGQDAINEFKLLIREAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKMNFWGYSTVGFFSPMIRYSSAGIRKCGQDAINEFKLLIREAHKRGIEVIM 360
Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
DVVFNHTAEGNENGP SFRGVDN+VYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNSVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420
Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
DPVLQGVK LIAEAWDAGGLY
Sbjct: 481 DPVLQGVK----------------------------------------LIAEAWDAGGLY 540
Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
QVGTFPHWGVWSEWNG+YRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWGVWSEWNGQYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
ICAHDGF+LADLVTYN+KHNLANGEDNNDGE+HNNSWNCGQEGEFVS+SVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNNKHNLANGEDNNDGESHNNSWNCGQEGEFVSMSVKKLRKRQMRN 660
Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
FFVCLMVSQ +NYFRWDKLEESSSDFHRFCSL+T
Sbjct: 661 FFVCLMVSQGVPMIHMGDEYGHTKGGNNNTYCHDNYINYFRWDKLEESSSDFHRFCSLVT 720
Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
TFR+ECESLGLNDFPT ERLQWHG+SPGKPDW+EASRFVAFTLVDSVK E+YIAFNA+HL
Sbjct: 721 TFRHECESLGLNDFPTAERLQWHGNSPGKPDWSEASRFVAFTLVDSVKGEIYIAFNANHL 780
Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 825
PVTV LPERPGYRW+PLVDTGKISPFDFL+SDLPERDVAI+QYAHFLD NLYPML YSSI
Sbjct: 781 PVTVTLPERPGYRWEPLVDTGKISPFDFLSSDLPERDVAIRQYAHFLDNNLYPMLCYSSI 808
BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match:
A0A6J1EW54 (isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 SV=1)
HSP 1 Score: 1443.7 bits (3736), Expect = 0.0e+00
Identity = 704/852 (82.63%), Postives = 735/852 (86.27%), Query Frame = 0
Query: 1 MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
MEL+TRSASLSSS SFL S F E RRR+SK NV+ RRRSDI DL V+ IHSR+N
Sbjct: 1 MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKHNVIPIRRRSDIHLDLHVDWIHSRINV 60
Query: 61 SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
S TRA +RGAEV T VT+AEEVE+SN NRFEVF+GTPTPFGAT R+GGINFAIYS NAVS
Sbjct: 61 SETRASSRGAEVRTSVTVAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
ATLCLINLFDLQ+ RV QEI LDPL N+TGDVWHVFLKGDFT+MLYGYKF GKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLINRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
YFDPSRILLDPYAKAVISR EFGALGP+DNCWPQMAGMVPTV++E FDWEGDLPLQH
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQHP 240
Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
QKDLIIYEMHVRGFTRHESSQS FPGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY+
Sbjct: 241 QKDLIIYEMHVRGFTRHESSQSGFPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYY 300
Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
SYNSVLGDYK+NFWGYSTV F SPMIRYSSAGI NCGQDAINEFKLLI+EAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIM 360
Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
DVVFNHTAEGNENGP SFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420
Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
LRYWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISN 480
Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
DP+LQGVK LIAEAWDAGGLY
Sbjct: 481 DPILQGVK----------------------------------------LIAEAWDAGGLY 540
Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
QVGTFPHW VWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
ICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
FFVCLMVSQ +NYFRWDK+EESSSDFHRFC LMT
Sbjct: 661 FFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFRWDKMEESSSDFHRFCRLMT 720
Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
TFRYECESLGLNDFPT +RLQWHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHL
Sbjct: 721 TFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHL 780
Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 826
PVTV LPERPGY+W+PLVDTGKISPFDFLTSDL ERDVAIQQYAHFLDANLYPMLSYSSI
Sbjct: 781 PVTVTLPERPGYQWEPLVDTGKISPFDFLTSDLEERDVAIQQYAHFLDANLYPMLSYSSI 809
BLAST of CmUC06G122320 vs. ExPASy TrEMBL
Match:
A0A6J1ICQ3 (isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=1)
HSP 1 Score: 1440.2 bits (3727), Expect = 0.0e+00
Identity = 704/852 (82.63%), Postives = 734/852 (86.15%), Query Frame = 0
Query: 1 MELITRSASLSSSASFLCDSKLRFLECRRRNSKQNVVRGRRRSDIRFDLDVNLIHSRMNA 60
MEL+TRSASLSSS SFL S F E RRR+SK+NV+ RRRSDI DLDV+ IHSR+N
Sbjct: 1 MELVTRSASLSSSVSFLRTSNSSFPEFRRRSSKRNVIPIRRRSDIHLDLDVDWIHSRINV 60
Query: 61 SVTRARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVS 120
S TRA +RGAEV T VTMAEEVE+SN NRFEVF+GTPTPFGAT R+GGINFAIYS NAVS
Sbjct: 61 SETRASSRGAEVRTSVTMAEEVEISNSNRFEVFEGTPTPFGATARDGGINFAIYSGNAVS 120
Query: 121 ATLCLINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGC 180
ATLCLINLFDLQ+ RV QEI LDPLTN+TGDVWHVFLKGDFT+MLYGYKF GKFSPQEGC
Sbjct: 121 ATLCLINLFDLQKKRVAQEITLDPLTNRTGDVWHVFLKGDFTDMLYGYKFGGKFSPQEGC 180
Query: 181 YFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHA 240
YFDPSRILLDPYAKAVISR EFGALGP+DNCWPQMAGMVPTV++E FDWEGDLPLQH
Sbjct: 181 YFDPSRILLDPYAKAVISREEFGALGPDDNCWPQMAGMVPTVDDE---FDWEGDLPLQHP 240
Query: 241 QKDLIIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYF 300
QKDLIIYEMHVRGFTRHESSQS PGTYLGLV+KLDYLKELGVNCIELMPCHEFNELEY+
Sbjct: 241 QKDLIIYEMHVRGFTRHESSQSGSPGTYLGLVDKLDYLKELGVNCIELMPCHEFNELEYY 300
Query: 301 SYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIM 360
SYNSVLGDYK+NFWGYSTV F SPMIRYSSAGI NCGQDAINEFKLLI+EAHKRGIEVIM
Sbjct: 301 SYNSVLGDYKVNFWGYSTVGFLSPMIRYSSAGIRNCGQDAINEFKLLIKEAHKRGIEVIM 360
Query: 361 DVVFNHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDC 420
DVVFNHTAEGNENGP SFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVR FIVDC
Sbjct: 361 DVVFNHTAEGNENGPIFSFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRRFIVDC 420
Query: 421 LRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISN 480
LRYWV EMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGD LTTGSPLGNPPLIDMISN
Sbjct: 421 LRYWVIEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDTLTTGSPLGNPPLIDMISN 480
Query: 481 DPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLY 540
DP+LQGVK LIAEAWDAGGLY
Sbjct: 481 DPILQGVK----------------------------------------LIAEAWDAGGLY 540
Query: 541 QVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
QVGTFPHW VWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF
Sbjct: 541 QVGTFPHWCVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINF 600
Query: 601 ICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
ICAHDGF+LADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN
Sbjct: 601 ICAHDGFTLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRN 660
Query: 661 FFVCLMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMT 720
FFVCLMVSQ +NYF WDK+EESSSDFHRFC LMT
Sbjct: 661 FFVCLMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYINYFLWDKMEESSSDFHRFCRLMT 720
Query: 721 TFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHL 780
TFRYECESLGLNDFPT +RLQWHGHSPG PDW+EASRFVAFTLVDSVKRE+YIAFNASHL
Sbjct: 721 TFRYECESLGLNDFPTAQRLQWHGHSPGNPDWSEASRFVAFTLVDSVKREIYIAFNASHL 780
Query: 781 PVTVMLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSI 826
PVTV LPER GYRW+PLVDTGKISPFDFLTSDL ERDVAIQQYAHFLDANLYPMLSYSSI
Sbjct: 781 PVTVTLPERLGYRWEPLVDTGKISPFDFLTSDLEERDVAIQQYAHFLDANLYPMLSYSSI 809
BLAST of CmUC06G122320 vs. TAIR 10
Match:
AT2G39930.1 (isoamylase 1 )
HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 534/784 (68.11%), Postives = 623/784 (79.46%), Query Frame = 0
Query: 65 ARNRGAEVGTKVTMAEEVEVSNLNRFEVFDGTPTPFGATTREGGINFAIYSANAVSATLC 124
A++R + + + E+ S +RF + DG P+PFG T R+ G+NF++YS N+VSAT+C
Sbjct: 44 AKDRRSNEAENIAVVEKPLKS--DRFFISDGLPSPFGPTVRDDGVNFSVYSTNSVSATIC 103
Query: 125 LINLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDP 184
LI+L DL++N+VT+EI+LDP N+TG VWHVFL+GDF +MLYGY+FDGKFSP+EG Y+D
Sbjct: 104 LISLSDLRQNKVTEEIQLDPSRNRTGHVWHVFLRGDFKDMLYGYRFDGKFSPEEGHYYDS 163
Query: 185 SRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDL 244
S ILLDPYAKA+ISR EFG LGP+DNCWPQMA MVPT EE FDWEGD+ L+ QKDL
Sbjct: 164 SNILLDPYAKAIISRDEFGVLGPDDNCWPQMACMVPTREEE---FDWEGDMHLKLPQKDL 223
Query: 245 IIYEMHVRGFTRHESSQSEFPGTYLGLVEKLDYLKELGVNCIELMPCHEFNELEYFSYNS 304
+IYEMHVRGFTRHESS+ EFPGTY G+ EKLD+LKELG+NCIELMPCHEFNELEY+SYN+
Sbjct: 224 VIYEMHVRGFTRHESSKIEFPGTYQGVAEKLDHLKELGINCIELMPCHEFNELEYYSYNT 283
Query: 305 VLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVF 364
+LGD+++NFWGYST+ FFSPMIRY+SA N AINEFK+L++EAHKRGIEVIMDVV
Sbjct: 284 ILGDHRVNFWGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVL 343
Query: 365 NHTAEGNENGPTISFRGVDNNVYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYW 424
NHTAEGNE GP SFRGVDN+VYYMLAP GEFYNYSGCGNTFNCNHP VR FI+DCLRYW
Sbjct: 344 NHTAEGNEKGPIFSFRGVDNSVYYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYW 403
Query: 425 VTEMHVDGFRFDLASIMTRGSSLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVL 484
VTEMHVDGFRFDL SIM+R SSLWDA NVYG +EGD+LTTG+P+ PP+IDMISNDP+L
Sbjct: 404 VTEMHVDGFRFDLGSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPIL 463
Query: 485 QGVKVFTLNSSLLLDCLLHGQFFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGT 544
+GVK LIAEAWDAGGLYQVG
Sbjct: 464 RGVK----------------------------------------LIAEAWDAGGLYQVGM 523
Query: 545 FPHWGVWSEWNGKYRDIVRQFIKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAH 604
FPHWG+WSEWNGK+RD+VRQFIKGTDGFSGAFAECLCGSPNLYQ GGRKPW+SINFICAH
Sbjct: 524 FPHWGIWSEWNGKFRDVVRQFIKGTDGFSGAFAECLCGSPNLYQ-GGRKPWHSINFICAH 583
Query: 605 DGFSLADLVTYNSKHNLANGEDNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNFFVC 664
DGF+LADLVTYN+K+NLANGE+NNDGENHN SWNCG+EG+F SISVK+LRKRQMRNFFV
Sbjct: 584 DGFTLADLVTYNNKNNLANGEENNDGENHNYSWNCGEEGDFASISVKRLRKRQMRNFFVS 643
Query: 665 LMVSQ---------------------------LNYFRWDKLEESSSDFHRFCSLMTTFRY 724
LMVSQ +NYFRWDK EE+ SDF RFC ++ FR
Sbjct: 644 LMVSQGVPMIYMGDEYGHTKGGNNNTYCHDNYMNYFRWDKKEEAHSDFFRFCRILIKFRD 703
Query: 725 ECESLGLNDFPTDERLQWHGHSPGKPDWTEASRFVAFTLVDSVKREVYIAFNASHLPVTV 784
ECESLGLNDFPT +RLQWHG +P P+W+E SRFVAF+LVDSVK+E+Y+AFN SHL V
Sbjct: 704 ECESLGLNDFPTAKRLQWHGLAPEIPNWSETSRFVAFSLVDSVKKEIYVAFNTSHLATLV 763
Query: 785 MLPERPGYRWDPLVDTGKISPFDFLTSDLPERDVAIQQYAHFLDANLYPMLSYSSIILLL 822
LP RPGYRW+P VDT K SP+D +T DLPER+ A++QY HFLDAN+YPMLSYSSIILLL
Sbjct: 764 SLPNRPGYRWEPFVDTSKPSPYDCITPDLPERETAMKQYRHFLDANVYPMLSYSSIILLL 781
BLAST of CmUC06G122320 vs. TAIR 10
Match:
AT4G09020.1 (isoamylase 3 )
HSP 1 Score: 542.7 bits (1397), Expect = 5.0e-154
Identity = 321/777 (41.31%), Postives = 425/777 (54.70%), Query Frame = 0
Query: 57 RMNASVTRARNRGAEVGTKVTM----------AEEVEVSNLNR---FEVFDGTPTPFGAT 116
+++ VTRAR ++ + T+ E E S + F+V G +P G +
Sbjct: 37 KLSRRVTRARIFSRKIKDRSTLKVTCRRAHERVVEEEASTMTETKLFKVSSGEVSPLGVS 96
Query: 117 TREGGINFAIYSANAVSATLCLI---NLFDLQENRVTQEIRLDPLTNKTGDVWHVFLKG- 176
+ GINFA++S NA S TLCL + D ++ E+ LDP NKTGD WH+ ++
Sbjct: 97 QVDKGINFALFSQNATSVTLCLSLSQSGKDDTDDDGMIELVLDPSVNKTGDTWHICVEDL 156
Query: 177 DFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFGALGPNDNCWPQMAGMV 236
+LYGY+ DG Q+G FD S +LLDPYAK V FG + + A
Sbjct: 157 PLNNVLYGYRVDGPGEWQQGHRFDRSILLLDPYAKLVKGHSSFG------DSSQKFAQFY 216
Query: 237 PTVNEEIKNFDWEGDLPLQH-AQKDLIIYEMHVRGFTRHESSQSE--FPGTYLGLVEKLD 296
T + E FDW D + +KDL+IYEM+VR FT ESS + G+YLG +EK+
Sbjct: 217 GTYDFESSPFDWGDDYKFPNIPEKDLVIYEMNVRAFTADESSGMDPAIGGSYLGFIEKIP 276
Query: 297 YLKELGVNCIELMPCHEFNELEYFSYNSVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNC 356
+L++LG+N +EL+P EF+ELE S D+ +N WGYSTVNFF+PM RY+S G G+
Sbjct: 277 HLQDLGINAVELLPVFEFDELE-LQRRSNPRDHMVNTWGYSTVNFFAPMSRYAS-GEGD- 336
Query: 357 GQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGP-TISFRGVDNNVYYMLAPMGE 416
A EFK +++ H GIEVI+DVV+NHT E ++ P T SFRG+DN VYYML P +
Sbjct: 337 PIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYPYTTSFRGIDNKVYYMLDPNNQ 396
Query: 417 FYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGSSLWDAVNVYG 476
N+SGCGNT NCNHP V I+D LR+WVTE HVDGFRFDLAS++ R
Sbjct: 397 LLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFRFDLASVLCRA----------- 456
Query: 477 NPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQFFAPVNKEAL 536
T GSPL PPLI I+ D VL K+
Sbjct: 457 --------TDGSPLSAPPLIRAIAKDSVLSRCKI-------------------------- 516
Query: 537 CSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQFIKGTDGFSGA 596
IAE WD GGLY VG FP+W W+EWNG YRD VR+FIKG G G+
Sbjct: 517 --------------IAEPWDCGGLYLVGKFPNWDRWAEWNGMYRDDVRRFIKGDSGMKGS 576
Query: 597 FAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGEDNNDGENHNN 656
FA + GS +LYQ RKP++ +NF+ AHDGF+L DLV+YN KHN ANGE NDG N N+
Sbjct: 577 FATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRDLVSYNFKHNEANGEGGNDGCNDNH 636
Query: 657 SWNCGQEGEFVSISVKKLRKRQMRNFFVCLMVSQ-------------------------- 716
SWNCG EGE +K LR RQM+NF + LM+SQ
Sbjct: 637 SWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGTPMMLMGDEYGHTRYGNNNSYGHDT 696
Query: 717 -LNYFRWDKLEESSSDFHRFCSLMTTFRYECESLGLNDFPTDERLQWHGHSPGKPDWTEA 776
LN F+W +L+ + RF S + FR+ L +F T + WH + +W +
Sbjct: 697 SLNNFQWKELDAKKQNHFRFFSEVIKFRHSHHVLKHENFLTQGEITWH-----EDNWDNS 740
Query: 777 -SRFVAFTLVDSV-KREVYIAFNASHLPVTVMLPE-RPGYRWDPLVDTGKISPFDFL 783
S+F+AFTL D + R++Y+AFNA V ++P+ PG +W + DT SP DF+
Sbjct: 757 ESKFLAFTLHDGIGGRDIYVAFNAHDYFVKALIPQPPPGKQWFRVADTNLESPDDFV 740
BLAST of CmUC06G122320 vs. TAIR 10
Match:
AT1G03310.2 (debranching enzyme 1 )
HSP 1 Score: 285.0 bits (728), Expect = 1.9e-76
Identity = 215/730 (29.45%), Postives = 331/730 (45.34%), Query Frame = 0
Query: 95 GTPTPFGATT--REGGINFAIYSANAVSATLCLINLFDLQENRVTQEIRLDPLTNKTGDV 154
G P P G ++ + NF+ +S ++ + LCL + D ++ E+ LDP N+TGDV
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYD--DSTTDKPALELDLDPYVNRTGDV 289
Query: 155 WHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAV---ISRGEFGALGPND 214
WH + + + YGY+ +E + I+LDPYA V +S+ G+L +
Sbjct: 290 WHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSLSKS- 349
Query: 215 NCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQ--SEFPGT 274
+FDW D+ + L++Y ++V+GFT+H SS+ S GT
Sbjct: 350 -----------------PSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGT 409
Query: 275 YLGLVEKLDYLKELGVNCIELMPCHEFNELE--YFSYNSVLGDYKMNFWGYSTVNFFSPM 334
+ G+ EK+ +LK LG N + L P F+E + YF + +FFSPM
Sbjct: 410 FSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPF-----------------HFFSPM 469
Query: 335 IRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVDNN 394
Y G N + A+N K+++++ H GIEV+++VVF HTA+ + RG+D++
Sbjct: 470 DIY---GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDS 529
Query: 395 VYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGS 454
YY + + S NCN+P V+ +++ LRYWVTE HVDGF F AS + RG
Sbjct: 530 SYYYKGRANDLDSKS----YLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRG- 589
Query: 455 SLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQ 514
G L PPL++ I+ DP+L K L+ DC
Sbjct: 590 ------------------VHGEQLSRPPLVEAIAFDPLLAETK-------LIADC----- 649
Query: 515 FFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQF 574
++ +E+ KE+ FPHW W+E N +Y VR F
Sbjct: 650 -------------WDPLEMMPKEV--------------RFPHWKRWAELNTRYCRNVRNF 709
Query: 575 IKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGE 634
++G G A +CGS +++ GR P S N+I + G SL D+V++ S LA+
Sbjct: 710 LRGR-GVLSDLATRICGSGDVFT-DGRGPAFSFNYISRNSGLSLVDIVSF-SGPELAS-- 769
Query: 635 DNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNF----FVCLMVSQLNY--------- 694
SWNCG+EG +V + R +Q+RNF ++ L V LN
Sbjct: 770 --------ELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTR 829
Query: 695 ----------FRWDKLEES-SSDFHRFCSLMTTFR-YECESLGLNDFPTDERLQWHGHSP 754
F W+ L + + +F S MT+ R + DF E + W+ +
Sbjct: 830 GSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQ 838
Query: 755 GKPDWTE-ASRFVAFTLVDSVKRE---------------VYIAFNASHLPVTVMLPERP- 774
P W + AS+F+A + + E ++I FNAS P +V+LP P
Sbjct: 890 TTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPD 838
BLAST of CmUC06G122320 vs. TAIR 10
Match:
AT1G03310.1 (debranching enzyme 1 )
HSP 1 Score: 285.0 bits (728), Expect = 1.9e-76
Identity = 215/730 (29.45%), Postives = 331/730 (45.34%), Query Frame = 0
Query: 95 GTPTPFGATT--REGGINFAIYSANAVSATLCLINLFDLQENRVTQEIRLDPLTNKTGDV 154
G P P G ++ + NF+ +S ++ + LCL + D ++ E+ LDP N+TGDV
Sbjct: 230 GHPLPLGLSSGPDDDSWNFSFFSRSSTNVVLCLYD--DSTTDKPALELDLDPYVNRTGDV 289
Query: 155 WHVFLKGDFTEMLYGYKFDGKFSPQEGCYFDPSRILLDPYAKAV---ISRGEFGALGPND 214
WH + + + YGY+ +E + I+LDPYA V +S+ G+L +
Sbjct: 290 WHASVDNTWDFVRYGYRCKETAHSKEDVDVEGEPIVLDPYATVVGKSVSQKYLGSLSKS- 349
Query: 215 NCWPQMAGMVPTVNEEIKNFDWEGDLPLQHAQKDLIIYEMHVRGFTRHESSQ--SEFPGT 274
+FDW D+ + L++Y ++V+GFT+H SS+ S GT
Sbjct: 350 -----------------PSFDWGEDVSPNIPLEKLLVYRLNVKGFTQHRSSKLPSNVAGT 409
Query: 275 YLGLVEKLDYLKELGVNCIELMPCHEFNELE--YFSYNSVLGDYKMNFWGYSTVNFFSPM 334
+ G+ EK+ +LK LG N + L P F+E + YF + +FFSPM
Sbjct: 410 FSGVAEKVSHLKTLGTNAVLLEPIFSFSEQKGPYFPF-----------------HFFSPM 469
Query: 335 IRYSSAGIGNCGQDAINEFKLLIREAHKRGIEVIMDVVFNHTAEGNENGPTISFRGVDNN 394
Y G N + A+N K+++++ H GIEV+++VVF HTA+ + RG+D++
Sbjct: 470 DIY---GPSNSLESAVNSMKVMVKKLHSEGIEVLLEVVFTHTADSG------ALRGIDDS 529
Query: 395 VYYMLAPMGEFYNYSGCGNTFNCNHPAVRMFIVDCLRYWVTEMHVDGFRFDLASIMTRGS 454
YY + + S NCN+P V+ +++ LRYWVTE HVDGF F AS + RG
Sbjct: 530 SYYYKGRANDLDSKS----YLNCNYPVVQQLVLESLRYWVTEFHVDGFCFINASSLLRG- 589
Query: 455 SLWDAVNVYGNPIEGDVLTTGSPLGNPPLIDMISNDPVLQGVKVFTLNSSLLLDCLLHGQ 514
G L PPL++ I+ DP+L K L+ DC
Sbjct: 590 ------------------VHGEQLSRPPLVEAIAFDPLLAETK-------LIADC----- 649
Query: 515 FFAPVNKEALCSAFNNVEIGAKELIAEAWDAGGLYQVGTFPHWGVWSEWNGKYRDIVRQF 574
++ +E+ KE+ FPHW W+E N +Y VR F
Sbjct: 650 -------------WDPLEMMPKEV--------------RFPHWKRWAELNTRYCRNVRNF 709
Query: 575 IKGTDGFSGAFAECLCGSPNLYQGGGRKPWNSINFICAHDGFSLADLVTYNSKHNLANGE 634
++G G A +CGS +++ GR P S N+I + G SL D+V++ S LA+
Sbjct: 710 LRGR-GVLSDLATRICGSGDVFT-DGRGPAFSFNYISRNSGLSLVDIVSF-SGPELAS-- 769
Query: 635 DNNDGENHNNSWNCGQEGEFVSISVKKLRKRQMRNF----FVCLMVSQLNY--------- 694
SWNCG+EG +V + R +Q+RNF ++ L V LN
Sbjct: 770 --------ELSWNCGEEGATNKSAVLQRRLKQIRNFLFIQYISLGVPVLNMGDECGISTR 829
Query: 695 ----------FRWDKLEES-SSDFHRFCSLMTTFR-YECESLGLNDFPTDERLQWHGHSP 754
F W+ L + + +F S MT+ R + DF E + W+ +
Sbjct: 830 GSPLLESRKPFDWNLLASAFGTQITQFISFMTSVRARRSDVFQRRDFLKPENIVWYANDQ 838
Query: 755 GKPDWTE-ASRFVAFTLVDSVKRE---------------VYIAFNASHLPVTVMLPERP- 774
P W + AS+F+A + + E ++I FNAS P +V+LP P
Sbjct: 890 TTPKWEDPASKFLALEIKSESEEEETASLAEPNEPKSNDLFIGFNASDHPESVVLPSLPD 838
BLAST of CmUC06G122320 vs. TAIR 10
Match:
AT5G04360.1 (limit dextrinase )
HSP 1 Score: 92.4 bits (228), Expect = 1.8e-18
Identity = 97/386 (25.13%), Postives = 154/386 (39.90%), Query Frame = 0
Query: 93 FDGTPTPFGATTREGGINFAIYSANAVSATLCLI-NLFDLQENRVTQEIRLDPLTNKTGD 152
+DG P GA G++ +++ A + ++C+ N D + + PL G
Sbjct: 204 YDG---PLGAHFTPEGVSLHLWAPTAQAVSVCIYKNPLD------KSPMEICPLKEANG- 263
Query: 153 VWHVFLKGDFTEMLYGYK---FDGKFSPQEGCYFDPSRILLDPYAKAVISRGEFGALGPN 212
VW + Y YK + E CY + DPYA+ + + G
Sbjct: 264 VWSTEGACSWGGCYYVYKVSVYHPSTMKLETCYAN------DPYARGLSADG-------- 323
Query: 213 DNCWPQMAGMVPTVNEEIKNFDWEG---DLPLQHAQKDLIIYEMHVRGFTRH-ESSQSEF 272
+ +V ++++K W+ P + D+ IYE+HVR F+ + E+ + E
Sbjct: 324 -----RKTFLVNLDSDDLKPEGWDNLADKKPCLRSFSDISIYELHVRDFSANDETVEPEN 383
Query: 273 PGTYLGLVEK-------LDYLKELGVNCIELMPCHEFNELEYFSYN-------------- 332
G YL K L L + G+ + L+P +F +++ N
Sbjct: 384 RGGYLAFTSKDSAGVKHLQKLVDAGLTHLHLLPTFQFGDVDDEKENWKSVDTSLLEGLRP 443
Query: 333 ----------SVLGDYKMNFWGYSTVNFFSPMIRYSSAGIGNCGQDAINEFKLLIREAHK 392
+ D N WGY+ V + P Y+S G C I EF+ +++ +
Sbjct: 444 DSTEAQARITEIQNDDGYN-WGYNPVLWGVPKGSYASDPTGPC---RIIEFRKMVQALNC 503
Query: 393 RGIEVIMDVVFNHTAEGNENGPTISFRGVDNNV--YYMLAPMGEFYNYSGCGNTFNCNHP 438
G+ V++DVV+NH + +GP +D V YY+ F S C N H
Sbjct: 504 TGLNVVLDVVYNHL---HASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTASEHY 553
The following BLAST results are available for this feature:
| Match Name | E-value | Identity | Description | |
|---|
| XP_038879251.1 | 0.0e+00 | 88.38 | isoamylase 1, chloroplastic [Benincasa hispida] | [more] |
| XP_008453530.1 | 0.0e+00 | 86.05 | PREDICTED: isoamylase 1, chloroplastic [Cucumis melo] >KAA0058172.1 isoamylase 1... | [more] |
| XP_004149676.1 | 0.0e+00 | 86.07 | isoamylase 1, chloroplastic [Cucumis sativus] >KAE8653176.1 hypothetical protein... | [more] |
| XP_022134724.1 | 0.0e+00 | 82.49 | isoamylase 1, chloroplastic [Momordica charantia] | [more] |
| XP_022932124.1 | 0.0e+00 | 82.63 | isoamylase 1, chloroplastic [Cucurbita moschata] | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| O04196 | 0.0e+00 | 68.11 | Isoamylase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA1 PE=1 SV=1 | [more] |
| D0TZF0 | 0.0e+00 | 64.03 | Isoamylase 1, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA1 PE=... | [more] |
| Q9M0S5 | 7.0e-153 | 41.31 | Isoamylase 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ISA3 PE=1 SV=2 | [more] |
| B9G434 | 1.3e-146 | 39.97 | Isoamylase 3, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=ISA3 PE=... | [more] |
| P0A4Y5 | 3.9e-111 | 40.94 | Glycogen operon protein GlgX homolog OS=Mycobacterium bovis (strain ATCC BAA-935... | [more] |
| Match Name | E-value | Identity | Description | |
|---|
| A0A5A7UQM5 | 0.0e+00 | 86.05 | Isoamylase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold274G004910 ... | [more] |
| A0A1S3BVW8 | 0.0e+00 | 86.05 | isoamylase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103494212 PE=4 SV=1 | [more] |
| A0A6J1BZK5 | 0.0e+00 | 82.49 | isoamylase 1, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111006927 PE=4 ... | [more] |
| A0A6J1EW54 | 0.0e+00 | 82.63 | isoamylase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111438446 PE=4 S... | [more] |
| A0A6J1ICQ3 | 0.0e+00 | 82.63 | isoamylase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111472580 PE=4 SV=... | [more] |
