CmUC06G112180 (gene) Watermelon (USVL531) v1

BLAST of CmUC06G112180 vs. NCBI nr
Match: XP_038875516.1 (tRNA pseudouridine synthase A 1 isoform X1 [Benincasa hispida])

HSP 1 Score: 660.6 bits (1703), Expect = 7.1e-186
Identity = 317/334 (94.91%), Postives = 324/334 (97.01%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITESPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLPLSP+IPFPNGASSSSSRG PN EDSS I   PFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVREISPA PDFHARFSTLSKVYHYKIYN AILDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVVNPVK IFRFDVI+MGPLVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIDMGPLVQ 240

Query: 241 IEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCLV 300
           +EVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILAS+DRRELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLV 300

Query: 301 SVNYKEDHLKLPSDCPGTSCGRSRSVSKCKLPFY 335
           S+NYKEDHLKLPSDCPGTS GR+RSVSKCKLPFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 334

BLAST of CmUC06G112180 vs. NCBI nr
Match: XP_008458279.1 (PREDICTED: tRNA pseudouridine synthase A 1 isoform X1 [Cucumis melo])

HSP 1 Score: 654.8 bits (1688), Expect = 3.9e-184
Identity = 311/334 (93.11%), Postives = 322/334 (96.41%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITESPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFPN EDSSYITE PFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIYN  I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RVVNPVK IFRFDVIEMG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 IEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCLV 300
           +EVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SVNYKEDHLKLPSDCPGTSCGRSRSVSKCKLPFY 335
           S+NYKEDHLKLPSDCPGTS GRSRSV KCK+PFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

BLAST of CmUC06G112180 vs. NCBI nr
Match: XP_004138518.2 (uncharacterized protein LOC101205797 [Cucumis sativus] >KGN45627.2 hypothetical protein Csa_005280 [Cucumis sativus])

HSP 1 Score: 646.0 bits (1665), Expect = 1.8e-181
Identity = 310/335 (92.54%), Postives = 322/335 (96.12%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVE-DSSYITESPFPSFKWRLLI 60
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRGFPN E D+SYI E  FP FKWRLLI
Sbjct: 21  MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 80

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 81  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 140

Query: 121 TPFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQ 180
           TPFNYDSLESVHAALNGLLPVDIRVRE+SPAVPDFHARFSTLSKVYHYKIY+  I+DPFQ
Sbjct: 141 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 200

Query: 181 RCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLV 240
           RCYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVVNPVK IFRFDVIEMG LV
Sbjct: 201 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 260

Query: 241 QIEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCL 300
           Q+EVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRRELAKYASGVPPQGLCL
Sbjct: 261 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 320

Query: 301 VSVNYKEDHLKLPSDCPGTSCGRSRSVSKCKLPFY 335
           VS+NYKEDHLKLPSDCPGTS GR+RSVSKCK+PFY
Sbjct: 321 VSINYKEDHLKLPSDCPGTSFGRTRSVSKCKVPFY 355

BLAST of CmUC06G112180 vs. NCBI nr
Match: XP_038875517.1 (tRNA pseudouridine synthase A 1 isoform X2 [Benincasa hispida])

HSP 1 Score: 637.9 bits (1644), Expect = 5.0e-179
Identity = 309/334 (92.51%), Postives = 315/334 (94.31%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITESPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLPLSP+IPFPNGASSSSSRG PN EDSS I   PFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPLSPAIPFPNGASSSSSRGSPNAEDSSCIIAPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVREISPA PDFHARFSTLSKVYHYKIYN AILDPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAAPDFHARFSTLSKVYHYKIYNGAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVVNPVK          GPLVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVK----------GPLVQ 240

Query: 241 IEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCLV 300
           +EVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILAS+DRRELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASQDRRELAKYASGVPPQGLCLV 300

Query: 301 SVNYKEDHLKLPSDCPGTSCGRSRSVSKCKLPFY 335
           S+NYKEDHLKLPSDCPGTS GR+RSVSKCKLPFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSVGRNRSVSKCKLPFY 324

BLAST of CmUC06G112180 vs. NCBI nr
Match: TYK02989.1 (protein TRIGALACTOSYLDIACYLGLYCEROL 4 [Cucumis melo var. makuwa])

HSP 1 Score: 630.6 bits (1625), Expect = 7.9e-177
Identity = 300/321 (93.46%), Postives = 310/321 (96.57%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITESPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFPN EDSSYITE PFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIYN  I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RVVNPVK IFRFDVIEMG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 IEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCLV 300
           +EVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SVNYKEDHLKLPSDCPGTSCG 322
           S+NYKEDHLKLPSDCPGTS G
Sbjct: 301 SINYKEDHLKLPSDCPGTSLG 321

BLAST of CmUC06G112180 vs. ExPASy Swiss-Prot
Match: Q6MEE7 (tRNA pseudouridine synthase A 1 OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=truA1 PE=3 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.3e-53
Identity = 105/255 (41.18%), Postives = 164/255 (64.31%), Query Frame = 0

Query: 55  WRLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQ 114
           ++L IAYDGT Y+GWQ Q +  +IQ  I+EAL     L+++ + LVG+ RTD GVHA GQ
Sbjct: 4   YKLTIAYDGTNYSGWQIQPNASSIQQKIQEAL--CILLKKEKVVLVGSGRTDAGVHAKGQ 63

Query: 115 VAHFITPFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAI 174
           VAHF    +Y  L  +H +LNGLLP DIR++ + P  P FH+++S + K YHY ++ + +
Sbjct: 64  VAHFHFQ-DYIDLSRLHVSLNGLLPRDIRIKAVEPVSPRFHSQYSAIRKEYHYYLHLNKV 123

Query: 175 LDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVV-NPVKDIFRFDVI 234
           +DPFQR Y++H   K++   +++AA  F G HDF++F N AH      NPV+ ++R D+ 
Sbjct: 124 MDPFQRLYSWHFQRKIDVNILKKAAILFTGTHDFTSFANEAHRGTAAKNPVRTLYRLDIK 183

Query: 235 EMGPLVQIEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVP 294
                +++E EG GFLY+ VRN+VG L+ +       + + +I A+++RR+ +  A   P
Sbjct: 184 PNEGGLRLEFEGDGFLYKMVRNIVGTLMDVASHKRAIEEINQIFAAKNRRQASLAA---P 243

Query: 295 PQGLCLVSVNYKEDH 309
           P+GL L+ V Y+ ++
Sbjct: 244 PEGLFLIQVFYENEN 252

BLAST of CmUC06G112180 vs. ExPASy Swiss-Prot
Match: B1I1B5 (tRNA pseudouridine synthase A OS=Desulforudis audaxviator (strain MP104C) OX=477974 GN=truA PE=3 SV=1)

HSP 1 Score: 192.6 bits (488), Expect = 7.2e-48
Identity = 109/251 (43.43%), Postives = 143/251 (56.97%), Query Frame = 0

Query: 56  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 115
           RL +AYDGT Y G+Q Q     PTIQ  +E  L    +L    L + GASRTD GVHA G
Sbjct: 5   RLTLAYDGTAYHGFQKQSGSGLPTIQETLERCL---AELSGAALKVTGASRTDAGVHARG 64

Query: 116 QVAHFITPFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSA 175
           QV +F+T       E + AALNG+LP DI   +      DFHAR+S ++K Y Y +YN  
Sbjct: 65  QVVNFVTGRWGIPTERIPAALNGVLPGDIAALDAREVPADFHARYSAVAKTYSYTLYNHP 124

Query: 176 ILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVI 235
           +  PFQR Y+ H    L+  AMR AA + +G+HDFS F  A     V + V+ + R D+ 
Sbjct: 125 VRSPFQRLYSLHVPRALDLRAMRLAARHLMGRHDFSTFQAAGR--PVQSAVRTLTRADIE 184

Query: 236 EMGPLVQIEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVP 295
              PLV++     GFLY  VR M G L+++G E + P+ +P I+AS DR   A+    VP
Sbjct: 185 AQAPLVRLVFRADGFLYNMVRIMAGTLLEVGLERLDPEALPDIIASGDR---ARAGPTVP 244

Query: 296 PQGLCLVSVNY 305
           P GLCL  V Y
Sbjct: 245 PHGLCLEEVEY 247

BLAST of CmUC06G112180 vs. ExPASy Swiss-Prot
Match: Q8R7Y7 (tRNA pseudouridine synthase A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=truA PE=3 SV=1)

HSP 1 Score: 188.0 bits (476), Expect = 1.8e-46
Identity = 110/248 (44.35%), Postives = 146/248 (58.87%), Query Frame = 0

Query: 57  LLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVA 116
           L+I YDGT Y GWQYQ++  T+Q ++E+A+    K+  + ++L+G+SRTD GVHA  QVA
Sbjct: 2   LIIEYDGTNYHGWQYQKNAITVQEVLEKAI---EKVTGEKVNLIGSSRTDTGVHALYQVA 61

Query: 117 HFITPFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILD 176
           +F T       E +  ALN +LP D+ V +       FHAR+S  SK Y Y I N     
Sbjct: 62  NFKTKTKIPP-EKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPM 121

Query: 177 PFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMG 236
           P  R Y +H  Y L+  AM++AA +F G HDFSAF   A  ++ VNPVK IF   V + G
Sbjct: 122 PTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAF--KASGNKKVNPVKTIFDLTVEKEG 181

Query: 237 PLVQIEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQG 296
            L++IEVE  GFLY  VR +VG LV +G   I    +P I+ S+DR    K     PPQG
Sbjct: 182 DLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDR---TKAGITAPPQG 240

Query: 297 LCLVSVNY 305
           L LV + Y
Sbjct: 242 LYLVRIVY 240

BLAST of CmUC06G112180 vs. ExPASy Swiss-Prot
Match: A3DJK8 (tRNA pseudouridine synthase A OS=Hungateiclostridium thermocellum (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) OX=203119 GN=truA PE=3 SV=1)

HSP 1 Score: 183.3 bits (464), Expect = 4.4e-45
Identity = 104/249 (41.77%), Postives = 145/249 (58.23%), Query Frame = 0

Query: 56  RLLIAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQV 115
           +L+I YDGT Y GWQ Q++  T+Q  IE A+ G T    + + L+GASRTD GVHA GQV
Sbjct: 5   KLIIEYDGTNYHGWQKQKNAKTVQDTIERAIKGLT---GEKVDLIGASRTDFGVHALGQV 64

Query: 116 AHFITPFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAIL 175
           A+FIT       +    ALN +LP DI ++E      DFHAR+    K Y Y IYNS   
Sbjct: 65  ANFITNSGIPG-DRFSYALNRMLPDDIVIKESQEVDMDFHARYKAKGKRYRYLIYNSQFP 124

Query: 176 DPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEM 235
               R  TYH  +KL++ +M+ AA+YF+G HDFSAF ++  + +    V+ I    +   
Sbjct: 125 SALLRHRTYHVSHKLDFESMQRAASYFLGTHDFSAFRSSGSSAK--TSVRTITDVSLERE 184

Query: 236 GPLVQIEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQ 295
             +V+ E+ G GFLY  VR +VG L+++G   I  D +P+I+ SR R+   + A   P +
Sbjct: 185 DKIVKFEIAGDGFLYNMVRIIVGTLMEVGIGKIRADEIPQIIESRKRKRAGRTA---PAE 244

Query: 296 GLCLVSVNY 305
           GL LV V Y
Sbjct: 245 GLYLVEVYY 244

BLAST of CmUC06G112180 vs. ExPASy Swiss-Prot
Match: A4J151 (tRNA pseudouridine synthase A OS=Desulfotomaculum reducens (strain MI-1) OX=349161 GN=truA PE=3 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 5.7e-45
Identity = 103/251 (41.04%), Postives = 147/251 (58.57%), Query Frame = 0

Query: 56  RLLIAYDGTRYAGWQYQRSP--PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWG 115
           +L +AYDGT Y G+Q QR     TIQ  +E+AL   + + +  + ++GA RTD GVHA G
Sbjct: 5   KLTLAYDGTNYHGFQEQRGTGLATIQEALEKAL---STIAQTPIQVIGAGRTDAGVHAQG 64

Query: 116 QVAHFITPFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSA 175
           QV +F +       E    ALN LLP DI+V +      DFHARFS ++K Y Y IY+  
Sbjct: 65  QVVNFRSEKWPVPAEKAPLALNVLLPGDIKVVKAEEVPMDFHARFSAVAKTYRYSIYHHR 124

Query: 176 ILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVI 235
           ++ PF R Y YH   +L+  AM+E A Y +G +DF +F   A    V + ++ I+R D+I
Sbjct: 125 VMSPFHRYYCYHEPRRLDVNAMQEGAAYLLGTYDFKSF--QAQGTPVKDTIRTIYRADII 184

Query: 236 EMGPLVQIEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVP 295
           E  P++ + + G+GFLY  VR + G L+ IG   I P+ + KI+ S++R      A   P
Sbjct: 185 EDAPVINLYLRGNGFLYNMVRIITGTLLNIGFGKIKPEDMVKIIESKNRTLAGTTA---P 244

Query: 296 PQGLCLVSVNY 305
           PQGLCL+ V Y
Sbjct: 245 PQGLCLMEVEY 247

BLAST of CmUC06G112180 vs. ExPASy TrEMBL
Match: A0A1S3C7L6 (tRNA pseudouridine synthase OS=Cucumis melo OX=3656 GN=LOC103497746 PE=3 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.9e-184
Identity = 311/334 (93.11%), Postives = 322/334 (96.41%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITESPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFPN EDSSYITE PFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIYN  I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RVVNPVK IFRFDVIEMG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 IEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCLV 300
           +EVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SVNYKEDHLKLPSDCPGTSCGRSRSVSKCKLPFY 335
           S+NYKEDHLKLPSDCPGTS GRSRSV KCK+PFY
Sbjct: 301 SINYKEDHLKLPSDCPGTSLGRSRSVGKCKVPFY 334

BLAST of CmUC06G112180 vs. ExPASy TrEMBL
Match: A0A5D3BY40 (Protein TRIGALACTOSYLDIACYLGLYCEROL 4 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold46G00780 PE=3 SV=1)

HSP 1 Score: 630.6 bits (1625), Expect = 3.8e-177
Identity = 300/321 (93.46%), Postives = 310/321 (96.57%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITESPFPSFKWRLLIA 60
           MRCWAVGSPVLPPISLP SP IP PNGASSSSSRGFPN EDSSYITE PFPSFKWRLLIA
Sbjct: 1   MRCWAVGSPVLPPISLPFSPIIPSPNGASSSSSRGFPNAEDSSYITEPPFPSFKWRLLIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+T
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFVT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQR 180
           PFNYDSLESVHAALNGLLPVDIRVRE+SPA PDFHARFSTLSKVYHYKIYN  I+DPFQR
Sbjct: 121 PFNYDSLESVHAALNGLLPVDIRVREMSPAAPDFHARFSTLSKVYHYKIYNGTIMDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHN+RVVNPVK IFRFDVIEMG LVQ
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNNRVVNPVKVIFRFDVIEMGALVQ 240

Query: 241 IEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCLV 300
           +EVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDR+ELAKYASGVPPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRKELAKYASGVPPQGLCLV 300

Query: 301 SVNYKEDHLKLPSDCPGTSCG 322
           S+NYKEDHLKLPSDCPGTS G
Sbjct: 301 SINYKEDHLKLPSDCPGTSLG 321

BLAST of CmUC06G112180 vs. ExPASy TrEMBL
Match: A0A0A0KB67 (tRNA pseudouridine synthase OS=Cucumis sativus OX=3659 GN=Csa_6G001730 PE=3 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 2.3e-174
Identity = 299/322 (92.86%), Postives = 309/322 (95.96%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVE-DSSYITESPFPSFKWRLLI 60
           MRCWAVGSPVLPPISLPLSP IP PNGASSSSSRGFPN E D+SYI E  FP FKWRLLI
Sbjct: 1   MRCWAVGSPVLPPISLPLSPIIPSPNGASSSSSRGFPNAEDDASYIKEPSFPCFKWRLLI 60

Query: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFI 120
           AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF+
Sbjct: 61  AYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFV 120

Query: 121 TPFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQ 180
           TPFNYDSLESVHAALNGLLPVDIRVRE+SPAVPDFHARFSTLSKVYHYKIY+  I+DPFQ
Sbjct: 121 TPFNYDSLESVHAALNGLLPVDIRVREMSPAVPDFHARFSTLSKVYHYKIYSGTIMDPFQ 180

Query: 181 RCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLV 240
           RCYTYHCVYKLNYAAM+EAANYFVGKHDFSAFTNAAHNDRVVNPVK IFRFDVIEMG LV
Sbjct: 181 RCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFTNAAHNDRVVNPVKVIFRFDVIEMGALV 240

Query: 241 QIEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCL 300
           Q+EVEGSGFLYRQVRNMVGLLVQIGKEA+PP+VVPKILASRDRRELAKYASGVPPQGLCL
Sbjct: 241 QLEVEGSGFLYRQVRNMVGLLVQIGKEALPPEVVPKILASRDRRELAKYASGVPPQGLCL 300

Query: 301 VSVNYKEDHLKLPSDCPGTSCG 322
           VS+NYKEDHLKLPSDCPGTS G
Sbjct: 301 VSINYKEDHLKLPSDCPGTSFG 322

BLAST of CmUC06G112180 vs. ExPASy TrEMBL
Match: A0A6J1L0L1 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 602.1 bits (1551), Expect = 1.5e-168
Identity = 288/334 (86.23%), Postives = 303/334 (90.72%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFPNVEDSSYITESPFPSFKWRLLIA 60
           M CWAVGS VLP +  PLSPSIP   G  SS+ RGFPN EDSS + E  FPSFKWRL+IA
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIP-SRGTLSSTGRGFPNSEDSSCVPEPDFPSFKWRLVIA 60

Query: 61  YDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFIT 120
           YDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAHFIT
Sbjct: 61  YDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHFIT 120

Query: 121 PFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQR 180
           PFNYDSL+ +HAALNGLLP DIRVREISPAVPDFHARFSTLSKVYHYKIYN AILDPFQR
Sbjct: 121 PFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPFQR 180

Query: 181 CYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLVQ 240
           CYTYHCVYKLNYAAM+EAANYFVGKHDFSAF N AHNDRVVNPVK IFRFDVIEMG L+Q
Sbjct: 181 CYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGALIQ 240

Query: 241 IEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLCLV 300
           +EVEGSGFLYRQVRNMV LL+QIGKEAIPPDVVPKILASRDR+ LAKY + +PPQGLCLV
Sbjct: 241 LEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLCLV 300

Query: 301 SVNYKEDHLKLPSDCPGTSCGRSRSVSKCKLPFY 335
            +NYKEDHLKLPSDCPGTS GRS SVSKCKLPFY
Sbjct: 301 RINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 333

BLAST of CmUC06G112180 vs. ExPASy TrEMBL
Match: A0A6J1L2M8 (tRNA pseudouridine synthase OS=Cucurbita maxima OX=3661 GN=LOC111499282 PE=3 SV=1)

HSP 1 Score: 597.0 bits (1538), Expect = 4.7e-167
Identity = 288/336 (85.71%), Postives = 303/336 (90.18%), Query Frame = 0

Query: 1   MRCWAVGSPVLPPISLPLSPSIPFPNGASSSSSRGFP--NVEDSSYITESPFPSFKWRLL 60
           M CWAVGS VLP +  PLSPSIP   G  SS+ RGFP  N EDSS + E  FPSFKWRL+
Sbjct: 1   MSCWAVGSAVLPSVPRPLSPSIP-SRGTLSSTGRGFPVMNSEDSSCVPEPDFPSFKWRLV 60

Query: 61  IAYDGTRYAGWQYQRSPPTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHF 120
           IAYDGTRYAGWQYQRSPPTIQCLIEEAL GAT+LERKDLHLVGASRTDKGVHAWGQVAHF
Sbjct: 61  IAYDGTRYAGWQYQRSPPTIQCLIEEALTGATRLERKDLHLVGASRTDKGVHAWGQVAHF 120

Query: 121 ITPFNYDSLESVHAALNGLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPF 180
           ITPFNYDSL+ +HAALNGLLP DIRVREISPAVPDFHARFSTLSKVYHYKIYN AILDPF
Sbjct: 121 ITPFNYDSLDRIHAALNGLLPADIRVREISPAVPDFHARFSTLSKVYHYKIYNDAILDPF 180

Query: 181 QRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPL 240
           QRCYTYHCVYKLNYAAM+EAANYFVGKHDFSAF N AHNDRVVNPVK IFRFDVIEMG L
Sbjct: 181 QRCYTYHCVYKLNYAAMKEAANYFVGKHDFSAFANTAHNDRVVNPVKVIFRFDVIEMGAL 240

Query: 241 VQIEVEGSGFLYRQVRNMVGLLVQIGKEAIPPDVVPKILASRDRRELAKYASGVPPQGLC 300
           +Q+EVEGSGFLYRQVRNMV LL+QIGKEAIPPDVVPKILASRDR+ LAKY + +PPQGLC
Sbjct: 241 IQLEVEGSGFLYRQVRNMVALLLQIGKEAIPPDVVPKILASRDRKVLAKYTTCIPPQGLC 300

Query: 301 LVSVNYKEDHLKLPSDCPGTSCGRSRSVSKCKLPFY 335
           LV +NYKEDHLKLPSDCPGTS GRS SVSKCKLPFY
Sbjct: 301 LVRINYKEDHLKLPSDCPGTSFGRSHSVSKCKLPFY 335

BLAST of CmUC06G112180 vs. TAIR 10
Match: AT3G06950.1 (Pseudouridine synthase family protein )

HSP 1 Score: 431.8 bits (1109), Expect = 5.0e-121
Identity = 212/320 (66.25%), Postives = 248/320 (77.50%), Query Frame = 0

Query: 17  PLSPSIPFPNG-ASSSSSRGFPNVEDSSYITESPFPSFKWRLLIAYDGTRYAGWQYQRSP 76
           P  P   F NG  SS  +    N+  S  +++S   ++KWRL+IAYDGTR+AGWQYQ SP
Sbjct: 6   PALPPSLFTNGYLSSPQNISMTNLNSSVKVSDS--GAYKWRLVIAYDGTRFAGWQYQESP 65

Query: 77  PTIQCLIEEALIGATKLERKDLHLVGASRTDKGVHAWGQVAHFITPFNYDSLESVHAALN 136
           PTIQ ++E+ALI  T+L RK+L L+GA RTD GVHAWGQVAHF+TPFNY SL+S HAALN
Sbjct: 66  PTIQSMLEKALIQITELGRKELQLIGAGRTDAGVHAWGQVAHFVTPFNYTSLDSFHAALN 125

Query: 137 GLLPVDIRVREISPAVPDFHARFSTLSKVYHYKIYNSAILDPFQRCYTYHCVYKLNYAAM 196
           GLLP DIRVRE+S AVP+FHARFS  SKVY Y+IYN   +DPFQR + YHC YKLN + M
Sbjct: 126 GLLPKDIRVRELSAAVPEFHARFSASSKVYRYQIYNDTFMDPFQRHWAYHCAYKLNASKM 185

Query: 197 REAANYFVGKHDFSAFTNAAHNDRVVNPVKDIFRFDVIEMGPLVQIEVEGSGFLYRQVRN 256
           REAAN FVGKHDFSAF NA   D V +P+K I RFDVI+MG L+Q+EVEGSGFLYRQVRN
Sbjct: 186 REAANLFVGKHDFSAFANATREDGVPDPLKTISRFDVIQMGSLLQLEVEGSGFLYRQVRN 245

Query: 257 MVGLLVQIGKEAIPPDVVPKILASRDRRELAKYAS-GVPPQGLCLVSVNYKEDHLKLPSD 316
           MV LL+QIGKEA+  D+VP IL ++DRR LAKY S    P GLCLVSV YKEDHLKLP D
Sbjct: 246 MVALLIQIGKEALDSDIVPMILETKDRRVLAKYTSLPASPHGLCLVSVKYKEDHLKLPLD 305

Query: 317 CPGTSCGRSRSVSKCKLPFY 335
           CP TS GR  +++KCKLPFY
Sbjct: 306 CPVTSSGRHHTITKCKLPFY 323

BLAST of CmUC06G112180 vs. TAIR 10
Match: AT1G34150.1 (Pseudouridine synthase family protein )

HSP 1 Score: 82.0 bits (201), Expect = 9.8e-16
Identity = 80/269 (29.74%), Postives = 113/269 (42.01%), Query Frame = 0

Query: 57  LLIAYDGTRYAGWQYQ-RSPPTIQCLIEEALIGATKL--ERKDLHLVGASRTDKGVHAWG 116
           L I Y G R+ G+  + +  P+I+  + +AL     L  ++KD       RTDKGV + G
Sbjct: 94  LKIMYFGKRFYGFSAEAQMEPSIESEVFKALERTRLLVGDKKDSCYSRCGRTDKGVSSTG 153

Query: 117 QVAHFITPFNY-----DSLESVHA------------ALNGLLPVDIRVREISPAVPDFHA 176
           QV              DS   V+              LN  LP DIRV   SPA  DFHA
Sbjct: 154 QVIALFLRSRLKSPPGDSKAQVNGRTGERPEYDYVRVLNRALPDDIRVIGWSPAPIDFHA 213

Query: 177 RFSTLSKVYHYKIYNSAILDPFQRCYTYHCVYKLNYAAMREAANYFVGKHDFSAFTNA-- 236
           RFS  ++ Y Y  +                   LN +AM  A   F+G+HDF  F     
Sbjct: 214 RFSCYAREYKYFFWRQ----------------NLNLSAMDFAGKKFIGEHDFRNFCKMDV 273

Query: 237 ----AHNDRV----VNPVKDIFRFDVIEMGPLVQIEVEGSGFLYRQVRNMVGLLVQIGKE 294
                +  RV    V+P ++    D      L    + GS FL+ Q+R MV +L  IG+ 
Sbjct: 274 ANVHCYTRRVTFFEVSPCQNSHEGD-----QLCTFTMRGSAFLWHQIRCMVAVLFMIGQG 333