Clc11G08700 (gene) Watermelon (cordophanus) v2

BLAST of Clc11G08700 vs. NCBI nr
Match: XP_038884332.1 (glucuronoxylan 4-O-methyltransferase 3-like [Benincasa hispida])

HSP 1 Score: 474.9 bits (1221), Expect = 5.0e-130
Identity = 244/295 (82.71%), Postives = 261/295 (88.47%), Query Frame = 0

Query: 16  MNMR-SRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQP---QTQV-----EPECP 75
           M+MR SR PLSINLKLILLLFLFLSFLLL+IRSGFQS  N  P   QT +     EPECP
Sbjct: 1   MSMRSSRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNSPPPATQTHLSNLTEEPECP 60

Query: 76  SSFPITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHD 135
           S  P T ICTKTP S+ANALIHYATTN+TPQQT  EISVSAT+L+ KSPCNFLVFGLGHD
Sbjct: 61  SP-PTTSICTKTPASVANALIHYATTNVTPQQTLKEISVSATILEAKSPCNFLVFGLGHD 120

Query: 136 SLMWTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEEC 195
           SLMWTALNHGGRTVFLEEDKAWI+QIQE +PNLEAYHVQYDTKV++ADELMKMGM EEEC
Sbjct: 121 SLMWTALNHGGRTVFLEEDKAWIEQIQERLPNLEAYHVQYDTKVHQADELMKMGM-EEEC 180

Query: 196 KSVGDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRG 255
           KSVGDPRFS+C+LALKGFP EIYE+EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR 
Sbjct: 181 KSVGDPRFSECQLALKGFPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRR 240

Query: 256 EGETDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           EGETDVFVHDVDRVVED+FSMAFLC+GYLRKQEGRIRHFTIP+HR    T PFCP
Sbjct: 241 EGETDVFVHDVDRVVEDSFSMAFLCQGYLRKQEGRIRHFTIPSHRPRPDT-PFCP 292

BLAST of Clc11G08700 vs. NCBI nr
Match: XP_004138670.1 (glucuronoxylan 4-O-methyltransferase 3 [Cucumis sativus] >KGN63058.1 hypothetical protein Csa_022287 [Cucumis sativus])

HSP 1 Score: 469.9 bits (1208), Expect = 1.6e-128
Identity = 243/293 (82.94%), Postives = 257/293 (87.71%), Query Frame = 0

Query: 16  MNMRS-RSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQP---QTQV---EPECPSS 75
           M+MRS R PLSINLKLILLLFLFLSFLLL+IRSGFQS  N  P   QT V   EPECPS 
Sbjct: 1   MSMRSTRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNNPPPVTQTHVLTDEPECPS- 60

Query: 76  FPITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSL 135
               PICTKTPPS+ANALIHYATTNITPQQT  EISVSA +L+TKSPCNFLVFGLGHDSL
Sbjct: 61  ---LPICTKTPPSVANALIHYATTNITPQQTLKEISVSARILETKSPCNFLVFGLGHDSL 120

Query: 136 MWTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKS 195
           MWTALNHGGRTVFLEEDKAWI QIQE +PNLEAYHVQYDTKV++ADELMK+GM  EECK 
Sbjct: 121 MWTALNHGGRTVFLEEDKAWIDQIQERLPNLEAYHVQYDTKVHQADELMKIGM-GEECKI 180

Query: 196 VGDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEG 255
           VGDPRF KC+LALKGFP EIYE+EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR EG
Sbjct: 181 VGDPRFCKCELALKGFPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQEG 240

Query: 256 ETDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           ETDVFVHDVDRVVEDNFSMAFLC+GYL KQEGRIRHF+IP+HRT   T PFCP
Sbjct: 241 ETDVFVHDVDRVVEDNFSMAFLCKGYLTKQEGRIRHFSIPSHRTRPDT-PFCP 287

BLAST of Clc11G08700 vs. NCBI nr
Match: XP_008441152.1 (PREDICTED: glucuronoxylan 4-O-methyltransferase 3-like [Cucumis melo])

HSP 1 Score: 469.2 bits (1206), Expect = 2.7e-128
Identity = 241/293 (82.25%), Postives = 258/293 (88.05%), Query Frame = 0

Query: 16  MNMR-SRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQP---QTQV---EPECPSS 75
           M+MR +R PLSINLKLILLLFLFLSFLLL+IRSGFQS  N  P   QT +   EPECPS 
Sbjct: 1   MSMRATRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNNPPPVTQTHILTDEPECPS- 60

Query: 76  FPITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSL 135
               PICTKTPPS+ANALIHYAT+NITPQQT  EISVSA +L+TKSPCNFLVFGLGHDSL
Sbjct: 61  ---LPICTKTPPSVANALIHYATSNITPQQTLKEISVSARILETKSPCNFLVFGLGHDSL 120

Query: 136 MWTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKS 195
           MWTALNHGGRTVFLEEDKAWI QIQE +PNLEAYHVQYDTKV++ADELMK+GM  EECKS
Sbjct: 121 MWTALNHGGRTVFLEEDKAWIDQIQERLPNLEAYHVQYDTKVHQADELMKIGM-GEECKS 180

Query: 196 VGDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEG 255
           VGDPRF KC+LALKG P EIYE+EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR EG
Sbjct: 181 VGDPRFCKCELALKGLPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQEG 240

Query: 256 ETDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           ETDVFVHDVDRVVEDNFSMAFLC+GYLRKQEGRIRHF+IP+HRT   T PFCP
Sbjct: 241 ETDVFVHDVDRVVEDNFSMAFLCKGYLRKQEGRIRHFSIPSHRTRPDT-PFCP 287

BLAST of Clc11G08700 vs. NCBI nr
Match: KAA0037074.1 (glucuronoxylan 4-O-methyltransferase 3-like [Cucumis melo var. makuwa] >TYK06575.1 glucuronoxylan 4-O-methyltransferase 3-like [Cucumis melo var. makuwa])

HSP 1 Score: 467.6 bits (1202), Expect = 8.0e-128
Identity = 238/288 (82.64%), Postives = 254/288 (88.19%), Query Frame = 0

Query: 20  SRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQP---QTQV---EPECPSSFPITP 79
           +R PLSINLKLILLLFLFLSFLLL+IRSGFQS  N  P   QT +   EPECPS     P
Sbjct: 4   TRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNNPPPVTQTHILTDEPECPS----LP 63

Query: 80  ICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTAL 139
           ICTKTPPS+ANALIHYAT+NITPQQT  EISVSA +L+TKSPCNFLVFGLGHDSLMWTAL
Sbjct: 64  ICTKTPPSVANALIHYATSNITPQQTLKEISVSARILETKSPCNFLVFGLGHDSLMWTAL 123

Query: 140 NHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSVGDPR 199
           NHGGRTVFLEEDKAWI QIQE +PNLEAYHVQYDTKV++ADELMK+GM  EECKSVGDPR
Sbjct: 124 NHGGRTVFLEEDKAWIDQIQERLPNLEAYHVQYDTKVHQADELMKIGM-GEECKSVGDPR 183

Query: 200 FSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEGETDVF 259
           F KC+LALKG P EIYE+EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR EGETDVF
Sbjct: 184 FCKCELALKGLPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQEGETDVF 243

Query: 260 VHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           VHDVDRVVEDNFSMAFLC+GYLRKQEGRIRHF+IP+HRT   T PFCP
Sbjct: 244 VHDVDRVVEDNFSMAFLCKGYLRKQEGRIRHFSIPSHRTRPDT-PFCP 285

BLAST of Clc11G08700 vs. NCBI nr
Match: XP_023550528.1 (glucuronoxylan 4-O-methyltransferase 3-like [Cucurbita pepo subsp. pepo] >KAG6578305.1 Glucuronoxylan 4-O-methyltransferase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 463.4 bits (1191), Expect = 1.5e-126
Identity = 237/294 (80.61%), Postives = 254/294 (86.39%), Query Frame = 0

Query: 16  MNMRSRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQ---PQTQV-----EPECPS 75
           M+MRSR PLSINLKLILLLF FLS  +L+IRSG QS  N      QT V     EP CP 
Sbjct: 1   MSMRSRPPLSINLKLILLLFFFLSIFMLVIRSGLQSSQNRPSPVAQTHVSNLTEEPACP- 60

Query: 76  SFPITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDS 135
           S P TP CTKTPPSLANALIHYATTNITPQQT  EISVSA VL+ KSPCNFLVFGLGHDS
Sbjct: 61  SLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARVLERKSPCNFLVFGLGHDS 120

Query: 136 LMWTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECK 195
           LMWTALNHGGRTVFLEEDKAWI QIQE +P+LEAY+VQYDTKV++A+ELMKMGM EEECK
Sbjct: 121 LMWTALNHGGRTVFLEEDKAWIDQIQERVPSLEAYNVQYDTKVHQAEELMKMGM-EEECK 180

Query: 196 SVGDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGE 255
           +VGDPRFSKC+LALKGFP EIY++EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR E
Sbjct: 181 TVGDPRFSKCQLALKGFPTEIYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQE 240

Query: 256 GETDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           G+TDVFVHDVDRVVED FSMAFLCEGYLRKQEGRIRHFTIP+HRT  +  PFCP
Sbjct: 241 GDTDVFVHDVDRVVEDKFSMAFLCEGYLRKQEGRIRHFTIPSHRTRGSR-PFCP 291

BLAST of Clc11G08700 vs. ExPASy Swiss-Prot
Match: Q9LQ32 (Glucuronoxylan 4-O-methyltransferase 3 OS=Arabidopsis thaliana OX=3702 GN=GXM3 PE=1 SV=1)

HSP 1 Score: 350.9 bits (899), Expect = 1.4e-95
Identity = 174/292 (59.59%), Postives = 217/292 (74.32%), Query Frame = 0

Query: 18  MRSRSPLSINLKLILL-LFLFLSFLLLLIRSGFQSPH-------NIQPQTQVEPECPSSF 77
           MR++SP S+NLK+I +   + +  ++ L RS   S         N+  + +         
Sbjct: 1   MRTKSPSSLNLKVIFIGSSILILIIIYLARSNISSSSSKPISKTNLSQEEEETQHKQEGC 60

Query: 78  PITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLM 137
           P T  CTK P SL++AL+HY T+N+TPQQTF+E+SVS  VL  KSPCNFLVFGLGHDSLM
Sbjct: 61  PTTQQCTKMPLSLSDALVHYVTSNVTPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLM 120

Query: 138 WTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSV 197
           W +LNHGGRT+F+EED+AWI  + +  PNLE+YHV YDTKV ++D+LM++G   EEC+SV
Sbjct: 121 WASLNHGGRTLFIEEDQAWIAIVTKKFPNLESYHVVYDTKVKDSDKLMELG-RSEECRSV 180

Query: 198 GDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEGE 257
            DPR SKC LALK FP + YE +WDLIMVDAPTGY +EAPGRMSAIYTAGL+ RNR +GE
Sbjct: 181 SDPRNSKCDLALKDFPADFYETKWDLIMVDAPTGYHEEAPGRMSAIYTAGLLARNREDGE 240

Query: 258 TDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           TDVFVHDV+R VED FS  FLC+GY+R+Q GR+RHFTIP+HR   A  PFCP
Sbjct: 241 TDVFVHDVNRPVEDEFSATFLCKGYMREQNGRLRHFTIPSHR-ARAGRPFCP 290

BLAST of Clc11G08700 vs. ExPASy Swiss-Prot
Match: Q9T0F7 (Glucuronoxylan 4-O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=GXM2 PE=1 SV=1)

HSP 1 Score: 339.7 bits (870), Expect = 3.3e-92
Identity = 170/288 (59.03%), Postives = 221/288 (76.74%), Query Frame = 0

Query: 18  MRSRSPLSINLKLILL-LFLFLSFLLLLIRSGF--QSPHNIQPQTQVEPECPSSFPITPI 77
           MR++S   I+ KLI +   + + F+L L R+ F   S   I+ +   + +CPS+      
Sbjct: 1   MRNKSQSFISSKLIFICCSILVLFILFLKRASFSSNSTATIRDEYHQKSKCPST---PQQ 60

Query: 78  CTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTALN 137
           CTK P SL++AL+HY T+ ITPQQTF+E+SVS  VL  KSPCNFLVFGLGHDSLMW +LN
Sbjct: 61  CTKLPTSLSDALVHYVTSEITPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLN 120

Query: 138 HGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSVGDPRF 197
           HGGRT+FLEED+AWI+ + +  PNLE+YHV YDTKV ++++LM++    E+CK+V DPR 
Sbjct: 121 HGGRTLFLEEDEAWIETVTKKFPNLESYHVVYDTKVKDSNKLMEL-KRTEDCKAVSDPRD 180

Query: 198 SKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGE-GETDVF 257
           SKC L+LKGFP ++YE +WD+IMVDAPTGY  EAPGRMSAIYTAGL+ RNR + GETDVF
Sbjct: 181 SKCALSLKGFPADVYETQWDVIMVDAPTGYHDEAPGRMSAIYTAGLLARNRYDGGETDVF 240

Query: 258 VHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           VHD++R VED FS+AFLC GY+++Q+GR+RHF IP+HR +  T PFCP
Sbjct: 241 VHDINRPVEDEFSVAFLCGGYMKEQQGRLRHFNIPSHRASFGT-PFCP 283

BLAST of Clc11G08700 vs. ExPASy Swiss-Prot
Match: Q6NMK1 (Glucuronoxylan 4-O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=GXM1 PE=1 SV=1)

HSP 1 Score: 334.7 bits (857), Expect = 1.1e-90
Identity = 171/289 (59.17%), Postives = 214/289 (74.05%), Query Frame = 0

Query: 18  MRSRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQ-PQTQVEPECPSSFPITPICT 77
           MR ++  +  LK++L+  L    L+ ++RS   S    Q PQ      C  +      C 
Sbjct: 1   MRPKANQNHKLKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRSTRCSGA------CN 60

Query: 78  KTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTALNHG 137
           K P SLA ALIHY+T+ ITPQQT  EI+VS+ VL  KSPCNFLVFGLGHDSLMW++LN+G
Sbjct: 61  KLPRSLAQALIHYSTSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSSLNYG 120

Query: 138 GRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSVGDPRFSK 197
           GRTVFLEED+AWI+QI+   P LE+YHV YD+KV +AD L+++G +  EC ++GDPR+S 
Sbjct: 121 GRTVFLEEDEAWIKQIKRRFPMLESYHVTYDSKVNQADNLIEVG-KGPECTAIGDPRYSM 180

Query: 198 CKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGE-GETDVFVH 257
           C+LALKG P EIYE  WDLIMVDAPTGY+ EAPGRM+AIYTAG+M RNR + GETDVFVH
Sbjct: 181 CQLALKGLPAEIYETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDVFVH 240

Query: 258 DVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTS---ATLPFCP 302
           DV+R +ED FS AFLCEGY++KQEGR+RHF IP++R  S   +  PFCP
Sbjct: 241 DVNREIEDKFSKAFLCEGYMKKQEGRLRHFIIPSYRDGSESESNRPFCP 282

BLAST of Clc11G08700 vs. ExPASy Swiss-Prot
Match: Q9C7F9 (Probable methyltransferase At1g27930 OS=Arabidopsis thaliana OX=3702 GN=At1g27930 PE=1 SV=1)

HSP 1 Score: 219.9 bits (559), Expect = 3.8e-56
Identity = 108/212 (50.94%), Postives = 141/212 (66.51%), Query Frame = 0

Query: 80  PSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTALNHGGRT 139
           P    A++HYAT+N+ PQQ   EIS+S  +L+  +P NFLVFGLG DSLMW +LN  G+T
Sbjct: 62  PIQLQAIVHYATSNVVPQQNLAEISISFNILKKLAPANFLVFGLGRDSLMWASLNPRGKT 121

Query: 140 VFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSVGD--PRFSKC 199
           +FLEED  W Q++ +  P L A+HV+Y T++ +AD L++    E +C           KC
Sbjct: 122 LFLEEDLEWFQKVTKDSPFLRAHHVRYRTQLQQADSLLRSYKTEPKCFPAKSYLRGNEKC 181

Query: 200 KLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGE-GETDVFVHD 259
           KLAL G P E Y+ EWDL+MVDAP GYF EAPGRM+AI++A +M RNR + G T VF+HD
Sbjct: 182 KLALTGLPDEFYDTEWDLLMVDAPKGYFAEAPGRMAAIFSAAVMARNRKKPGVTHVFLHD 241

Query: 260 VDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIP 289
           V+R VE  F+  FLC  Y     GR+ HF IP
Sbjct: 242 VNRRVEKTFAEEFLCRKYRVNAAGRLWHFAIP 273

BLAST of Clc11G08700 vs. ExPASy Swiss-Prot
Match: Q9SNE5 (Protein IRREGULAR XYLEM 15 OS=Arabidopsis thaliana OX=3702 GN=IRX15 PE=2 SV=1)

HSP 1 Score: 170.2 bits (430), Expect = 3.5e-41
Identity = 88/215 (40.93%), Postives = 130/215 (60.47%), Query Frame = 0

Query: 79  PPSLANALIHYAT-TNITPQQTFNEI-SVSATVLQTKSPCNFLVFGLGHDSLMWTALNHG 138
           P S  NAL+HYA+ +N +   ++ E+ S+S  + +   PCN LVFGL H++L+W +LNH 
Sbjct: 85  PTSAINALLHYASRSNDSFHMSYGEMKSISDVLRRCAPPCNLLVFGLTHETLLWKSLNHN 144

Query: 139 GRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEE--EECKSVGDPRF 198
           GRTVF+EE++ +    +E  P ++ + VQY TK +EA EL+    E    EC+ V +  F
Sbjct: 145 GRTVFIEENRYYAAYFEEIHPEIDVFDVQYTTKAHEAGELVTAAKEAAGNECRPVQNLLF 204

Query: 199 SKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNR--GEGETDV 258
           S CKL L   P  +Y+++WD+I VD P G   E PGRMS+I+TA ++ R++  G  +T V
Sbjct: 205 SDCKLGLNDLPNHVYDVDWDVIFVDGPRGDAHEGPGRMSSIFTAAVLARSKKGGTPKTHV 264

Query: 259 FVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTI 288
           FVHD  R VE      FLC   L +    + H+ +
Sbjct: 265 FVHDYYRDVERLCGDEFLCRENLVESNDLLAHYVL 299

BLAST of Clc11G08700 vs. ExPASy TrEMBL
Match: A0A0A0LMN7 (Polysacc_synt_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G396200 PE=4 SV=1)

HSP 1 Score: 469.9 bits (1208), Expect = 7.8e-129
Identity = 243/293 (82.94%), Postives = 257/293 (87.71%), Query Frame = 0

Query: 16  MNMRS-RSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQP---QTQV---EPECPSS 75
           M+MRS R PLSINLKLILLLFLFLSFLLL+IRSGFQS  N  P   QT V   EPECPS 
Sbjct: 1   MSMRSTRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNNPPPVTQTHVLTDEPECPS- 60

Query: 76  FPITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSL 135
               PICTKTPPS+ANALIHYATTNITPQQT  EISVSA +L+TKSPCNFLVFGLGHDSL
Sbjct: 61  ---LPICTKTPPSVANALIHYATTNITPQQTLKEISVSARILETKSPCNFLVFGLGHDSL 120

Query: 136 MWTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKS 195
           MWTALNHGGRTVFLEEDKAWI QIQE +PNLEAYHVQYDTKV++ADELMK+GM  EECK 
Sbjct: 121 MWTALNHGGRTVFLEEDKAWIDQIQERLPNLEAYHVQYDTKVHQADELMKIGM-GEECKI 180

Query: 196 VGDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEG 255
           VGDPRF KC+LALKGFP EIYE+EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR EG
Sbjct: 181 VGDPRFCKCELALKGFPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQEG 240

Query: 256 ETDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           ETDVFVHDVDRVVEDNFSMAFLC+GYL KQEGRIRHF+IP+HRT   T PFCP
Sbjct: 241 ETDVFVHDVDRVVEDNFSMAFLCKGYLTKQEGRIRHFSIPSHRTRPDT-PFCP 287

BLAST of Clc11G08700 vs. ExPASy TrEMBL
Match: A0A1S3B2B1 (glucuronoxylan 4-O-methyltransferase 3-like OS=Cucumis melo OX=3656 GN=LOC103485376 PE=4 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 1.3e-128
Identity = 241/293 (82.25%), Postives = 258/293 (88.05%), Query Frame = 0

Query: 16  MNMR-SRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQP---QTQV---EPECPSS 75
           M+MR +R PLSINLKLILLLFLFLSFLLL+IRSGFQS  N  P   QT +   EPECPS 
Sbjct: 1   MSMRATRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNNPPPVTQTHILTDEPECPS- 60

Query: 76  FPITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSL 135
               PICTKTPPS+ANALIHYAT+NITPQQT  EISVSA +L+TKSPCNFLVFGLGHDSL
Sbjct: 61  ---LPICTKTPPSVANALIHYATSNITPQQTLKEISVSARILETKSPCNFLVFGLGHDSL 120

Query: 136 MWTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKS 195
           MWTALNHGGRTVFLEEDKAWI QIQE +PNLEAYHVQYDTKV++ADELMK+GM  EECKS
Sbjct: 121 MWTALNHGGRTVFLEEDKAWIDQIQERLPNLEAYHVQYDTKVHQADELMKIGM-GEECKS 180

Query: 196 VGDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEG 255
           VGDPRF KC+LALKG P EIYE+EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR EG
Sbjct: 181 VGDPRFCKCELALKGLPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQEG 240

Query: 256 ETDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           ETDVFVHDVDRVVEDNFSMAFLC+GYLRKQEGRIRHF+IP+HRT   T PFCP
Sbjct: 241 ETDVFVHDVDRVVEDNFSMAFLCKGYLRKQEGRIRHFSIPSHRTRPDT-PFCP 287

BLAST of Clc11G08700 vs. ExPASy TrEMBL
Match: A0A5A7T0Q8 (Glucuronoxylan 4-O-methyltransferase 3-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold453G00390 PE=4 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 3.9e-128
Identity = 238/288 (82.64%), Postives = 254/288 (88.19%), Query Frame = 0

Query: 20  SRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQP---QTQV---EPECPSSFPITP 79
           +R PLSINLKLILLLFLFLSFLLL+IRSGFQS  N  P   QT +   EPECPS     P
Sbjct: 4   TRPPLSINLKLILLLFLFLSFLLLVIRSGFQSSQNNPPPVTQTHILTDEPECPS----LP 63

Query: 80  ICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTAL 139
           ICTKTPPS+ANALIHYAT+NITPQQT  EISVSA +L+TKSPCNFLVFGLGHDSLMWTAL
Sbjct: 64  ICTKTPPSVANALIHYATSNITPQQTLKEISVSARILETKSPCNFLVFGLGHDSLMWTAL 123

Query: 140 NHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSVGDPR 199
           NHGGRTVFLEEDKAWI QIQE +PNLEAYHVQYDTKV++ADELMK+GM  EECKSVGDPR
Sbjct: 124 NHGGRTVFLEEDKAWIDQIQERLPNLEAYHVQYDTKVHQADELMKIGM-GEECKSVGDPR 183

Query: 200 FSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEGETDVF 259
           F KC+LALKG P EIYE+EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR EGETDVF
Sbjct: 184 FCKCELALKGLPSEIYEMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQEGETDVF 243

Query: 260 VHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           VHDVDRVVEDNFSMAFLC+GYLRKQEGRIRHF+IP+HRT   T PFCP
Sbjct: 244 VHDVDRVVEDNFSMAFLCKGYLRKQEGRIRHFSIPSHRTRPDT-PFCP 285

BLAST of Clc11G08700 vs. ExPASy TrEMBL
Match: A0A6J1FGV0 (glucuronoxylan 4-O-methyltransferase 3-like OS=Cucurbita moschata OX=3662 GN=LOC111445277 PE=4 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.6e-126
Identity = 236/294 (80.27%), Postives = 254/294 (86.39%), Query Frame = 0

Query: 16  MNMRSRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQ---PQTQV-----EPECPS 75
           M+MRSR PLSINLKLILLLF FLS  +L+I+SG QS  N      QT V     EP CP 
Sbjct: 1   MSMRSRPPLSINLKLILLLFFFLSIFMLVIKSGLQSSQNRPSPVAQTHVSNLTEEPACP- 60

Query: 76  SFPITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDS 135
           S P TP CTKTPPSLANALIHYATTNITPQQT  EISVSA VL+ KSPCNFLVFGLGHDS
Sbjct: 61  SLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARVLERKSPCNFLVFGLGHDS 120

Query: 136 LMWTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECK 195
           LMWTALNHGGRTVFLEEDKAWI QIQE +P+LEAY+VQYDTKV++A+ELMKMGM EEECK
Sbjct: 121 LMWTALNHGGRTVFLEEDKAWIDQIQERVPSLEAYNVQYDTKVHQAEELMKMGM-EEECK 180

Query: 196 SVGDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGE 255
           +VGDPRFSKC+LALKGFP EIY++EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR E
Sbjct: 181 TVGDPRFSKCQLALKGFPTEIYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQE 240

Query: 256 GETDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           G+TDVFVHDVDRVVED FSMAFLCEGYLRKQEGRIRHFTIP+HRT  +  PFCP
Sbjct: 241 GDTDVFVHDVDRVVEDKFSMAFLCEGYLRKQEGRIRHFTIPSHRTRGSR-PFCP 291

BLAST of Clc11G08700 vs. ExPASy TrEMBL
Match: A0A6J1K144 (glucuronoxylan 4-O-methyltransferase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111489201 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.8e-126
Identity = 236/294 (80.27%), Postives = 253/294 (86.05%), Query Frame = 0

Query: 16  MNMRSRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQ---PQTQV-----EPECPS 75
           M+MRSR PLSINLKLILLLF FLS  +L+IRSG QS  N      QT V     EP CP 
Sbjct: 1   MSMRSRPPLSINLKLILLLFFFLSIFMLVIRSGLQSSQNRPSPVAQTHVSNLTEEPACP- 60

Query: 76  SFPITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDS 135
           S P TP CTKTPPSLANALIHYATTNITPQQT  EISVSA VL+ KSPCNFLVFGLGHDS
Sbjct: 61  SLPFTPSCTKTPPSLANALIHYATTNITPQQTLKEISVSARVLERKSPCNFLVFGLGHDS 120

Query: 136 LMWTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECK 195
           LMWTALNHGGRTVFLEEDKAWI QIQE +P LEAY+VQYDTKV++A+ELMK+GM EEECK
Sbjct: 121 LMWTALNHGGRTVFLEEDKAWIDQIQERVPTLEAYNVQYDTKVHQAEELMKIGM-EEECK 180

Query: 196 SVGDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGE 255
           +VGDPRFSKC+LALKGFP EIY++EWDLIMVDAPTGYF EAPGRMSAIYTAGLM RNR E
Sbjct: 181 TVGDPRFSKCQLALKGFPTEIYDMEWDLIMVDAPTGYFNEAPGRMSAIYTAGLMARNRQE 240

Query: 256 GETDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           G+TDVFVHDVDRVVED FSMAFLCEGYLRKQEGRIRHFTIP+HRT  +  PFCP
Sbjct: 241 GDTDVFVHDVDRVVEDKFSMAFLCEGYLRKQEGRIRHFTIPSHRTRGSR-PFCP 291

BLAST of Clc11G08700 vs. TAIR 10
Match: AT1G33800.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 350.9 bits (899), Expect = 1.0e-96
Identity = 174/292 (59.59%), Postives = 217/292 (74.32%), Query Frame = 0

Query: 18  MRSRSPLSINLKLILL-LFLFLSFLLLLIRSGFQSPH-------NIQPQTQVEPECPSSF 77
           MR++SP S+NLK+I +   + +  ++ L RS   S         N+  + +         
Sbjct: 1   MRTKSPSSLNLKVIFIGSSILILIIIYLARSNISSSSSKPISKTNLSQEEEETQHKQEGC 60

Query: 78  PITPICTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLM 137
           P T  CTK P SL++AL+HY T+N+TPQQTF+E+SVS  VL  KSPCNFLVFGLGHDSLM
Sbjct: 61  PTTQQCTKMPLSLSDALVHYVTSNVTPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLM 120

Query: 138 WTALNHGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSV 197
           W +LNHGGRT+F+EED+AWI  + +  PNLE+YHV YDTKV ++D+LM++G   EEC+SV
Sbjct: 121 WASLNHGGRTLFIEEDQAWIAIVTKKFPNLESYHVVYDTKVKDSDKLMELG-RSEECRSV 180

Query: 198 GDPRFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEGE 257
            DPR SKC LALK FP + YE +WDLIMVDAPTGY +EAPGRMSAIYTAGL+ RNR +GE
Sbjct: 181 SDPRNSKCDLALKDFPADFYETKWDLIMVDAPTGYHEEAPGRMSAIYTAGLLARNREDGE 240

Query: 258 TDVFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           TDVFVHDV+R VED FS  FLC+GY+R+Q GR+RHFTIP+HR   A  PFCP
Sbjct: 241 TDVFVHDVNRPVEDEFSATFLCKGYMREQNGRLRHFTIPSHR-ARAGRPFCP 290

BLAST of Clc11G08700 vs. TAIR 10
Match: AT4G09990.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 339.7 bits (870), Expect = 2.3e-93
Identity = 170/288 (59.03%), Postives = 221/288 (76.74%), Query Frame = 0

Query: 18  MRSRSPLSINLKLILL-LFLFLSFLLLLIRSGF--QSPHNIQPQTQVEPECPSSFPITPI 77
           MR++S   I+ KLI +   + + F+L L R+ F   S   I+ +   + +CPS+      
Sbjct: 1   MRNKSQSFISSKLIFICCSILVLFILFLKRASFSSNSTATIRDEYHQKSKCPST---PQQ 60

Query: 78  CTKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTALN 137
           CTK P SL++AL+HY T+ ITPQQTF+E+SVS  VL  KSPCNFLVFGLGHDSLMW +LN
Sbjct: 61  CTKLPTSLSDALVHYVTSEITPQQTFDEVSVSKRVLDKKSPCNFLVFGLGHDSLMWASLN 120

Query: 138 HGGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSVGDPRF 197
           HGGRT+FLEED+AWI+ + +  PNLE+YHV YDTKV ++++LM++    E+CK+V DPR 
Sbjct: 121 HGGRTLFLEEDEAWIETVTKKFPNLESYHVVYDTKVKDSNKLMEL-KRTEDCKAVSDPRD 180

Query: 198 SKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGE-GETDVF 257
           SKC L+LKGFP ++YE +WD+IMVDAPTGY  EAPGRMSAIYTAGL+ RNR + GETDVF
Sbjct: 181 SKCALSLKGFPADVYETQWDVIMVDAPTGYHDEAPGRMSAIYTAGLLARNRYDGGETDVF 240

Query: 258 VHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSATLPFCP 302
           VHD++R VED FS+AFLC GY+++Q+GR+RHF IP+HR +  T PFCP
Sbjct: 241 VHDINRPVEDEFSVAFLCGGYMKEQQGRLRHFNIPSHRASFGT-PFCP 283

BLAST of Clc11G08700 vs. TAIR 10
Match: AT1G09610.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 334.7 bits (857), Expect = 7.5e-92
Identity = 171/289 (59.17%), Postives = 214/289 (74.05%), Query Frame = 0

Query: 18  MRSRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQ-PQTQVEPECPSSFPITPICT 77
           MR ++  +  LK++L+  L    L+ ++RS   S    Q PQ      C  +      C 
Sbjct: 1   MRPKANQNHKLKVLLVFLLATLILIFIVRSTLTSSQEHQTPQETRSTRCSGA------CN 60

Query: 78  KTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTALNHG 137
           K P SLA ALIHY+T+ ITPQQT  EI+VS+ VL  KSPCNFLVFGLGHDSLMW++LN+G
Sbjct: 61  KLPRSLAQALIHYSTSVITPQQTLKEIAVSSRVLGKKSPCNFLVFGLGHDSLMWSSLNYG 120

Query: 138 GRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSVGDPRFSK 197
           GRTVFLEED+AWI+QI+   P LE+YHV YD+KV +AD L+++G +  EC ++GDPR+S 
Sbjct: 121 GRTVFLEEDEAWIKQIKRRFPMLESYHVTYDSKVNQADNLIEVG-KGPECTAIGDPRYSM 180

Query: 198 CKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGE-GETDVFVH 257
           C+LALKG P EIYE  WDLIMVDAPTGY+ EAPGRM+AIYTAG+M RNR + GETDVFVH
Sbjct: 181 CQLALKGLPAEIYETGWDLIMVDAPTGYYDEAPGRMTAIYTAGMMARNRKQGGETDVFVH 240

Query: 258 DVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTS---ATLPFCP 302
           DV+R +ED FS AFLCEGY++KQEGR+RHF IP++R  S   +  PFCP
Sbjct: 241 DVNREIEDKFSKAFLCEGYMKKQEGRLRHFIIPSYRDGSESESNRPFCP 282

BLAST of Clc11G08700 vs. TAIR 10
Match: AT1G71690.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 310.1 bits (793), Expect = 2.0e-84
Identity = 160/288 (55.56%), Postives = 199/288 (69.10%), Query Frame = 0

Query: 18  MRSRSPLSINLKLILLLFLFLSFLLLLIRSGFQSPHNIQPQTQVEPECPSSFPITPICTK 77
           M +R  LS  + L++L   F++       S F  P N   +        S       C K
Sbjct: 9   MTTRLILSSFVSLVVLTLFFITRTGFSPSSSFHQPLNNTLRISTS-STGSKLQSPRSCNK 68

Query: 78  TPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTALNHGG 137
            PPSLA+AL+HYA++N+TPQQT +EISV+   L+ KSPCNFLVFGLGHDSLMW  LNHGG
Sbjct: 69  IPPSLADALVHYASSNVTPQQTLSEISVTKKELEKKSPCNFLVFGLGHDSLMWATLNHGG 128

Query: 138 RTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEECKSVG-DPRFSK 197
           RT+FL+ED++WI QI E  P+LE+YHV+Y TKV +A+ LM    + EEC+ V  D R S 
Sbjct: 129 RTIFLDEDESWIHQIAEKFPSLESYHVRYKTKVRDAEALMAATKDREECRRVSTDLRVST 188

Query: 198 CKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNRGEGE--TDVFV 257
           C+LALKG P  +YE EWDLIMVDAPTG+ +EAPGRMSAIYTAG++ R R + E  T VFV
Sbjct: 189 CELALKGLPEVVYETEWDLIMVDAPTGFHEEAPGRMSAIYTAGMIARRRKDEEETTAVFV 248

Query: 258 HDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIPTHRTTSAT-LPFCP 302
           HDVDR VED FSM FLC  Y+ KQEGR+RHFT+P+HR    + + FCP
Sbjct: 249 HDVDRKVEDEFSMEFLCRDYMTKQEGRLRHFTVPSHRNYGVSGVKFCP 295

BLAST of Clc11G08700 vs. TAIR 10
Match: AT1G67330.1 (Protein of unknown function (DUF579) )

HSP 1 Score: 228.4 bits (581), Expect = 7.6e-60
Identity = 116/217 (53.46%), Postives = 146/217 (67.28%), Query Frame = 0

Query: 76  TKTPPSLANALIHYATTNITPQQTFNEISVSATVLQTKSPCNFLVFGLGHDSLMWTALNH 135
           T TP  L  +++HYAT++  PQQ+F EIS+S  VL+ + PCNFLVFGLG DSLMW +LN 
Sbjct: 62  TATPIQL-QSIVHYATSHTVPQQSFEEISISLNVLKERLPCNFLVFGLGRDSLMWASLNP 121

Query: 136 GGRTVFLEEDKAWIQQIQEGMPNLEAYHVQYDTKVYEADELMKMGMEEEEC---KSVGDP 195
           GG TVFLEED  WI+ + +  P+L A+HVQY T + EA  L+     E  C   K+    
Sbjct: 122 GGTTVFLEEDPEWIEAVLKDAPSLRAHHVQYRTHLSEAGRLLSTYKNEPMCLPAKAFPIR 181

Query: 196 RFSKCKLALKGFPREIYEIEWDLIMVDAPTGYFKEAPGRMSAIYTAGLMGRNR-GEGETD 255
              KC LAL   P E Y+ EWDLIMVDAP GYF EAPGRM+AI+++ +M RNR G+G T 
Sbjct: 182 YNEKCPLALTSLPDEFYDTEWDLIMVDAPKGYFPEAPGRMAAIFSSAIMARNRKGDGTTH 241

Query: 256 VFVHDVDRVVEDNFSMAFLCEGYLRKQEGRIRHFTIP 289
           VF+HDV+R VE+ F+  FLCE Y     GR+ HF IP
Sbjct: 242 VFLHDVNRKVENAFANEFLCEKYKVNSVGRLWHFEIP 277