Clc10G11470 (gene) Watermelon (cordophanus) v2

Overview


Name	Clc10G11470
Type	gene
Organism	Citrullus lanatus subsp. cordophanus cv. cordophanus (Watermelon (cordophanus) v2)
Description	RNA/RNP complex-1-interacting phosphatase, putative
Location	ClcChr10: 24902850 .. 24903616 (+)
RNA-Seq Expression	Clc10G11470
Synteny	Clc10G11470

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AAAAAACCATTGAAAAATCCTTTGCAAAAGTATGCCAATTCCAAACTTTATTATTGTCTATACATAACTAAAATTATCCATTCCTTTTTCCAATTTATTCAAATTCCCTCTCATCCCCACAAAAATATCTCCCAACTCCCAGCCCATTCCTCCTGCCATTTCCATGGCAGCCCTCTCCGCCGGCCACCGCCATTTCTTCCAACCACAAAGCTGCAGCGCCACCGTGCTGTTCGGAAACAGAAGCATCAGCCCCAGATCCATCGCCCTCCTCTGCTGCCATCCTTCAGATCCTTCAGATTCTTCAGAAGAAAAAACGAAGAAGAAAGCCCAAGAAAGAAAACAGATGCTTCCTGGGTTTTTCCAGAGATTTGAGAAGATGGGAATTGAATTGAAGGAGAAATTGAGTCCTCAAATAAAGGGGGATTGGAAGGATGTGACACTGATGAGCCTTTCATTTGCTGTTTATGTATATATATCTCAGAAGATTGTTTGTGCTTATTTTCTTTGGATGTCAATGCCGAAACAACTGTGGTAGAAGATGGTGTATTTCAAAGGGCTCCTTCTTGTTGATGATTGAAATCTGTGTATGATTAAAGTTTACTCTTCCTTGGAATAGAAATGTAATTTGATTACTTACTTGTGAATATATATAGACGCATTTCGTCATATAAACATGTAGATTGGACGGTTAAATTGTAGAGTGAAAGTTTGGATCATCTACCTTTTAATCAGATGCATGCATTTAGACATATTTGTTAAGAAGAGAG

mRNA sequence

AAAAAACCATTGAAAAATCCTTTGCAAAAGTATGCCAATTCCAAACTTTATTATTGTCTATACATAACTAAAATTATCCATTCCTTTTTCCAATTTATTCAAATTCCCTCTCATCCCCACAAAAATATCTCCCAACTCCCAGCCCATTCCTCCTGCCATTTCCATGGCAGCCCTCTCCGCCGGCCACCGCCATTTCTTCCAACCACAAAGCTGCAGCGCCACCGTGCTGTTCGGAAACAGAAGCATCAGCCCCAGATCCATCGCCCTCCTCTGCTGCCATCCTTCAGATCCTTCAGATTCTTCAGAAGAAAAAACGAAGAAGAAAGCCCAAGAAAGAAAACAGATGCTTCCTGGGTTTTTCCAGAGATTTGAGAAGATGGGAATTGAATTGAAGGAGAAATTGAGTCCTCAAATAAAGGGGGATTGGAAGGATGTGACACTGATGAGCCTTTCATTTGCTGTTTATGTATATATATCTCAGAAGATTGTTTGTGCTTATTTTCTTTGGATGTCAATGCCGAAACAACTGTGGTAGAAGATGGTGTATTTCAAAGGGCTCCTTCTTGTTGATGATTGAAATCTGTGTATGATTAAAGTTTACTCTTCCTTGGAATAGAAATGTAATTTGATTACTTACTTGTGAATATATATAGACGCATTTCGTCATATAAACATGTAGATTGGACGGTTAAATTGTAGAGTGAAAGTTTGGATCATCTACCTTTTAATCAGATGCATGCATTTAGACATATTTGTTAAGAAGAGAG

Coding sequence (CDS)

ATGGCAGCCCTCTCCGCCGGCCACCGCCATTTCTTCCAACCACAAAGCTGCAGCGCCACCGTGCTGTTCGGAAACAGAAGCATCAGCCCCAGATCCATCGCCCTCCTCTGCTGCCATCCTTCAGATCCTTCAGATTCTTCAGAAGAAAAAACGAAGAAGAAAGCCCAAGAAAGAAAACAGATGCTTCCTGGGTTTTTCCAGAGATTTGAGAAGATGGGAATTGAATTGAAGGAGAAATTGAGTCCTCAAATAAAGGGGGATTGGAAGGATGTGACACTGATGAGCCTTTCATTTGCTGTTTATGTATATATATCTCAGAAGATTGTTTGTGCTTATTTTCTTTGGATGTCAATGCCGAAACAACTGTGGTAG

Protein sequence

MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCHPSDPSDSSEEKTKKKAQERKQMLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSMPKQLW

Homology

[Close]

BLAST of Clc10G11470 vs. NCBI nr
Match: KGN58582.1 (hypothetical protein Csa_001602 [Cucumis sativus])

HSP 1 Score: 202.6 bits (514), Expect = 2.0e-48
Identity = 103/126 (81.75%), Postives = 113/126 (89.68%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCH---PSDPSDSSEEKTKKKAQE 60
           MAALSAGHRHFF  QS SAT+LF NRSISPRSI++LC H   PSDP+D SEEKTKKK+QE
Sbjct: 1   MAALSAGHRHFFYSQSGSATLLFRNRSISPRSISVLCLHNQDPSDPTDCSEEKTKKKSQE 60

Query: 61  RKQMLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMS 120
           RK+M+ GF QRFEKMGI+LKEKLSPQ KGDWKD+TLMSLSFAVYVYISQKIVCAYFLWM+
Sbjct: 61  RKEMVHGFVQRFEKMGIQLKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFLWMT 120

Query: 121 MPKQLW 124
           MPK LW
Sbjct: 121 MPKPLW 126

[Close]

BLAST of Clc10G11470 vs. NCBI nr
Match: KAA0048367.1 (putative RNA/RNP complex-1-interacting phosphatase [Cucumis melo var. makuwa] >TYK04067.1 putative RNA/RNP complex-1-interacting phosphatase [Cucumis melo var. makuwa])

HSP 1 Score: 197.6 bits (501), Expect = 6.3e-47
Identity = 102/126 (80.95%), Postives = 110/126 (87.30%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCH---PSDPSDSSEEKTKKKAQE 60
           MAALSAGHRHFF   S SA V F NRSISPRSIA+LC H   PSDP+D S+EKTKKK+QE
Sbjct: 1   MAALSAGHRHFFYSLSGSAIVQFRNRSISPRSIAVLCLHNQDPSDPTDCSDEKTKKKSQE 60

Query: 61  RKQMLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMS 120
           RK+M+ GF QRFEKMGI+LKEKLSPQ KGDWKD+TLMSLSFAVYVYISQKIVCAYFLWMS
Sbjct: 61  RKEMVHGFVQRFEKMGIQLKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFLWMS 120

Query: 121 MPKQLW 124
           MPK LW
Sbjct: 121 MPKPLW 126

[Close]

BLAST of Clc10G11470 vs. NCBI nr
Match: KAG6581569.1 (hypothetical protein SDJN03_21571, partial [Cucurbita argyrosperma subsp. sororia] >KAG7018076.1 hypothetical protein SDJN02_19942, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 189.1 bits (479), Expect = 2.2e-44
Identity = 101/125 (80.80%), Postives = 105/125 (84.00%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCC--HPSDPSDSSEEKTKKKAQER 60
           MAALSAGHRHFF        V FGNRSISPR I LLCC  H  DPSDSSEEKTKK  QE 
Sbjct: 1   MAALSAGHRHFF------PAVQFGNRSISPRPIVLLCCHNHNQDPSDSSEEKTKKD-QEG 60

Query: 61  KQMLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSM 120
           KQM+PGF QR++KMG+ELKEKLSPQ KGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSM
Sbjct: 61  KQMVPGFIQRYKKMGMELKEKLSPQRKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSM 118

Query: 121 PKQLW 124
           PKQLW
Sbjct: 121 PKQLW 118

[Close]

BLAST of Clc10G11470 vs. NCBI nr
Match: KAG6606884.1 (hypothetical protein SDJN03_00226, partial [Cucurbita argyrosperma subsp. sororia] >KAG7036590.1 hypothetical protein SDJN02_00209, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 185.7 bits (470), Expect = 2.5e-43
Identity = 100/126 (79.37%), Postives = 107/126 (84.92%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCH---PSDPSDSSEEKTKKKAQE 60
           MAALSAGHR+FF     SA V F NRSISPRSI LLC H   PSDPSD SEEK KK+ QE
Sbjct: 1   MAALSAGHRYFFG----SAAVHFRNRSISPRSIVLLCLHNQNPSDPSDPSEEKPKKE-QE 60

Query: 61  RKQMLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMS 120
           +KQ +PGF QRFEKMGIELKEKLSPQ KGDWKD+TLMSLSFAVYVYISQKIVCAYF+WMS
Sbjct: 61  KKQSIPGFVQRFEKMGIELKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFVWMS 120

Query: 121 MPKQLW 124
           MPKQ+W
Sbjct: 121 MPKQVW 121

[Close]

BLAST of Clc10G11470 vs. NCBI nr
Match: XP_022152649.1 (uncharacterized protein LOC111020315 [Momordica charantia])

HSP 1 Score: 153.7 bits (387), Expect = 1.0e-33
Identity = 85/124 (68.55%), Postives = 98/124 (79.03%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCHPSDPSDSSEEKTKKKAQERKQ 60
           MAALSAGHRHFF+ +  +A  L   RS SP+SI LLC H  DP   SEE +K+  + ++Q
Sbjct: 1   MAALSAGHRHFFR-RGAAAQQLRTRRS-SPKSIVLLCRHSQDP---SEENSKEGEERKQQ 60

Query: 61  MLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSMPK 120
            + GF +RFEKMG+ELKEKLSPQ KGDWKDV LMSLSFAVYVYISQKIVCAYF+WMSMPK
Sbjct: 61  KIGGFVERFEKMGMELKEKLSPQRKGDWKDVALMSLSFAVYVYISQKIVCAYFVWMSMPK 119

Query: 121 -QLW 124
            QLW
Sbjct: 121 QQLW 119

[Close]

BLAST of Clc10G11470 vs. ExPASy TrEMBL
Match: A0A0A0LD14 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G690300 PE=4 SV=1)

HSP 1 Score: 202.6 bits (514), Expect = 9.5e-49
Identity = 103/126 (81.75%), Postives = 113/126 (89.68%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCH---PSDPSDSSEEKTKKKAQE 60
           MAALSAGHRHFF  QS SAT+LF NRSISPRSI++LC H   PSDP+D SEEKTKKK+QE
Sbjct: 1   MAALSAGHRHFFYSQSGSATLLFRNRSISPRSISVLCLHNQDPSDPTDCSEEKTKKKSQE 60

Query: 61  RKQMLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMS 120
           RK+M+ GF QRFEKMGI+LKEKLSPQ KGDWKD+TLMSLSFAVYVYISQKIVCAYFLWM+
Sbjct: 61  RKEMVHGFVQRFEKMGIQLKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFLWMT 120

Query: 121 MPKQLW 124
           MPK LW
Sbjct: 121 MPKPLW 126

[Close]

BLAST of Clc10G11470 vs. ExPASy TrEMBL
Match: A0A5D3BX35 (Putative RNA/RNP complex-1-interacting phosphatase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1871G00020 PE=4 SV=1)

HSP 1 Score: 197.6 bits (501), Expect = 3.1e-47
Identity = 102/126 (80.95%), Postives = 110/126 (87.30%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCH---PSDPSDSSEEKTKKKAQE 60
           MAALSAGHRHFF   S SA V F NRSISPRSIA+LC H   PSDP+D S+EKTKKK+QE
Sbjct: 1   MAALSAGHRHFFYSLSGSAIVQFRNRSISPRSIAVLCLHNQDPSDPTDCSDEKTKKKSQE 60

Query: 61  RKQMLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMS 120
           RK+M+ GF QRFEKMGI+LKEKLSPQ KGDWKD+TLMSLSFAVYVYISQKIVCAYFLWMS
Sbjct: 61  RKEMVHGFVQRFEKMGIQLKEKLSPQRKGDWKDLTLMSLSFAVYVYISQKIVCAYFLWMS 120

Query: 121 MPKQLW 124
           MPK LW
Sbjct: 121 MPKPLW 126

[Close]

BLAST of Clc10G11470 vs. ExPASy TrEMBL
Match: A0A6J1DGN1 (uncharacterized protein LOC111020315 OS=Momordica charantia OX=3673 GN=LOC111020315 PE=4 SV=1)

HSP 1 Score: 153.7 bits (387), Expect = 5.1e-34
Identity = 85/124 (68.55%), Postives = 98/124 (79.03%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCHPSDPSDSSEEKTKKKAQERKQ 60
           MAALSAGHRHFF+ +  +A  L   RS SP+SI LLC H  DP   SEE +K+  + ++Q
Sbjct: 1   MAALSAGHRHFFR-RGAAAQQLRTRRS-SPKSIVLLCRHSQDP---SEENSKEGEERKQQ 60

Query: 61  MLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSMPK 120
            + GF +RFEKMG+ELKEKLSPQ KGDWKDV LMSLSFAVYVYISQKIVCAYF+WMSMPK
Sbjct: 61  KIGGFVERFEKMGMELKEKLSPQRKGDWKDVALMSLSFAVYVYISQKIVCAYFVWMSMPK 119

Query: 121 -QLW 124
            QLW
Sbjct: 121 QQLW 119

[Close]

BLAST of Clc10G11470 vs. ExPASy TrEMBL
Match: A0A660KVF0 (Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_010795 PE=4 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 6.6e-26
Identity = 70/123 (56.91%), Postives = 82/123 (66.67%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCHPSDPSDSSEEKTKKKAQERKQ 60
           MAALS GH    +P     T     +S SP ++ L CC    P DSS  +TKK+  +RK 
Sbjct: 1   MAALSIGHSFHREP----ITYQQLRKSSSPTTLILSCCQRHKPVDSSNPRTKKEKSKRKL 60

Query: 61  MLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSMPK 120
           +L      FEK G  L+E LSP+ KGDWKD+TLMSLSFAVYVYISQK+VCAYF WMSMPK
Sbjct: 61  LLQRSSVGFEKFGKILRENLSPKQKGDWKDLTLMSLSFAVYVYISQKLVCAYFAWMSMPK 119

Query: 121 QLW 124
           QLW
Sbjct: 121 QLW 119

[Close]

BLAST of Clc10G11470 vs. ExPASy TrEMBL
Match: A0A6P9E117 (uncharacterized protein LOC118344507 OS=Juglans regia OX=51240 GN=LOC118344507 PE=4 SV=1)

HSP 1 Score: 119.0 bits (297), Expect = 1.4e-23
Identity = 67/123 (54.47%), Postives = 81/123 (65.85%), Query Frame = 0


Query: 1   MAALSAGHRHFFQPQSCSATVLFGNRSISPRSIALLCCHPSDPSDSSEEKTKKKAQERKQ 60
           MAAL  GH  + +      T+    +  SPR+  LL C    P+D+S  +TKK+++ R+ 
Sbjct: 1   MAALLVGHSLYRR----ICTIQEPRKKSSPRTFILLGCQSHKPADTSNPRTKKESR-RES 60

Query: 61  MLPGFFQRFEKMGIELKEKLSPQIKGDWKDVTLMSLSFAVYVYISQKIVCAYFLWMSMPK 120
           +LPG    F K G  LKE +SPQ KGDWKDV LMSLSFAVYVYISQKIVCAY  WMSMPK
Sbjct: 61  LLPGILVGFGKFGKVLKENMSPQQKGDWKDVMLMSLSFAVYVYISQKIVCAYCAWMSMPK 118

Query: 121 QLW 124
           Q W
Sbjct: 121 QPW 118

[Close]

BLAST of Clc10G11470 vs. TAIR 10
Match: AT3G52070.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 23 Blast hits to 23 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 59.7 bits (143), Expect = 1.9e-09
Identity = 39/77 (50.65%), Postives = 45/77 (58.44%), Query Frame = 0


Query: 58  RKQMLP----GFFQRFEKMGIELKEKLSPQI-----KGDWKDVTLMSLSFAVYVYISQKI 117
           RKQ LP    G  QR  +  I    K+   +     KGD KD+ LMSLSFAVYVYISQ +
Sbjct: 38  RKQKLPEENEGVIQRTLRRMISEAGKIGKNLKPEKKKGDVKDLMLMSLSFAVYVYISQLL 97

Query: 118 VCAYFLW--MSMPKQLW 124
           VCAYF W  +S PK  W
Sbjct: 98  VCAYFSWQHLSFPKSSW 114

[Close]

BLAST of Clc10G11470 vs. TAIR 10
Match: AT3G52070.2 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: chloroplast; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 23 Blast hits to 23 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 57.0 bits (136), Expect = 1.2e-08
Identity = 38/74 (51.35%), Postives = 44/74 (59.46%), Query Frame = 0


Query: 58  RKQMLP----GFFQRFEKMGIELKEKLSPQI-----KGDWKDVTLMSLSFAVYVYISQKI 117
           RKQ LP    G  QR  +  I    K+   +     KGD KD+ LMSLSFAVYVYISQ +
Sbjct: 38  RKQKLPEENEGVIQRTLRRMISEAGKIGKNLKPEKKKGDVKDLMLMSLSFAVYVYISQLL 97

Query: 118 VCAYFLW--MSMPK 121
           VCAYF W  +S PK
Sbjct: 98  VCAYFSWQHLSFPK 111

The following BLAST results are available for this feature:

BLAST of Clc10G11470 vs. NCBI nr


Match Name	E-value	Identity	Description
KGN58582.1	2.0e-48	81.75	hypothetical protein Csa_001602 [Cucumis sativus]	[more]
KAA0048367.1	6.3e-47	80.95	putative RNA/RNP complex-1-interacting phosphatase [Cucumis melo var. makuwa] >T...	[more]
KAG6581569.1	2.2e-44	80.80	hypothetical protein SDJN03_21571, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KAG6606884.1	2.5e-43	79.37	hypothetical protein SDJN03_00226, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022152649.1	1.0e-33	68.55	uncharacterized protein LOC111020315 [Momordica charantia]	[more]

BLAST of Clc10G11470 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Clc10G11470 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LD14	9.5e-49	81.75	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G690300 PE=4 SV=1	[more]
A0A5D3BX35	3.1e-47	80.95	Putative RNA/RNP complex-1-interacting phosphatase OS=Cucumis melo var. makuwa O...	[more]
A0A6J1DGN1	5.1e-34	68.55	uncharacterized protein LOC111020315 OS=Momordica charantia OX=3673 GN=LOC111020...	[more]
A0A660KVF0	6.6e-26	56.91	Uncharacterized protein OS=Carpinus fangiana OX=176857 GN=FH972_010795 PE=4 SV=1	[more]
A0A6P9E117	1.4e-23	54.47	uncharacterized protein LOC118344507 OS=Juglans regia OX=51240 GN=LOC118344507 P...	[more]

BLAST of Clc10G11470 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52070.1	1.9e-09	50.65	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT3G52070.2	1.2e-08	51.35	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Watermelon (cordophanus) v2
Date Performed: 2022-01-31


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 43..58
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 39..58

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Clc10G11470.1	Clc10G11470.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane